Search

Your search keyword '"Nakamura, Tatsuo"' showing total 1,037 results
Start Over Author "Nakamura, Tatsuo"
1,037 results on '"Nakamura, Tatsuo"'

209. Drug delivery system of hepatocyte treatment of vocal fold scarring growth factor for the in a canine model

Author

10635519, 00738247, 70227908, 50211371, Ohno, Tsunehisa, Hirano, Shigeru, Kanemaru, Shin-Ichi, Yamashita, Masaru, Umeda, Hiroo, Suehiro, Atsushi, Tamura, Yoshihiro, Nakamura, Tatsuo, Ito, Juichi, Tabata, Yasuhiko, 10635519, 00738247, 70227908, 50211371, Ohno, Tsunehisa, Hirano, Shigeru, Kanemaru, Shin-Ichi, Yamashita, Masaru, Umeda, Hiroo, Suehiro, Atsushi, Tamura, Yoshihiro, Nakamura, Tatsuo, Ito, Juichi, and Tabata, Yasuhiko

Published
2007

211. Comparison of ASCs and BMSCs combined with atelocollagen for vocal fold scar regeneration.

Author

Hiwatashi, Nao, Hirano, Shigeru, Suzuki, Ryo, Kawai, Yoshitaka, Mizuta, Masanobu, Kishimoto, Yo, Tateya, Ichiro, Kanemaru, Shin‐ichi, Nakamura, Tatsuo, Dezawa, Mari, and Ito, Juichi

Abstract

Objectives/hypothesis: Vocal fold scar remains a therapeutic challenge. Mesenchymal stromal cells (MSCs) are promising tools for regenerative medicine. Nevertheless, few in vivo studies have directly compared various sources of MSCs. The aim of this study was to investigate the therapeutic potential of adipose-derived stromal cells (ASCs) in comparison with bone marrow-derived stromal cells (BMSCs) for vocal fold regeneration.Study Design: Prospective animal experiments with controls.Methods: Two months after stripping of the lamina propria, 18 beagles were divided into four implantation groups: atelocollagen alone (collagen group), atelocollagen with BMSCs (BMSC-collagen), atelocollagen with ASCs (ASC-collagen), or a sham-treated group. One or 6 months after implantation, vibratory and histological examinations were performed.Results: Mucosal vibration was significantly improved in both of the MSC-implanted groups compared with the sham-treated group, whereas only the ASC-collagen group showed a significantly smaller glottal gap than the collagen group. Moreover, in the ASC-collagen group, a significant reduction of collagen density was observed compared to the sham-treated group, and there was a trend for better restoration of hyaluronic acid (HA). Implanted MSCs were detected 1 month postimplantation; however, none survived 6 months postimplantation.Conclusions: Although implantation of an atelocollagen sponge and ASCs into vocal fold scars induced vibratory recovery comparable to that of BMSCs, ASCs might have more potential in terms of restoration of HA and suppression of excessive collagen deposition.Level Of Evidence: NA Laryngoscope, 126:1143-1150, 2016. [ABSTRACT FROM AUTHOR]

Published
2016
Full Text
View/download PDF

212. Effect of Structural Differences in Collagen Sponge Scaffolds on Tracheal Epithelium Regeneration.

Author

Nakaegawa, Yuta, Nakamura, Ryosuke, Tada, Yasuhiro, Nomoto, Yukio, Imaizumi, Mitsuyoshi, Suzuki, Ryo, Nakamura, Tatsuo, and Omori, Koichi

Subjects
BRONCHOSCOPY, COLLAGEN, REGENERATION (Biology), SCANNING electron microscopy, TRACHEAL diseases, TISSUE engineering
Abstract

Objective: We developed an in situ regeneration-inducible artificial trachea composed of a porcine collagen sponge and polypropylene framework and used it for tracheal reconstruction. In the present study, collagen sponges with different structures were prepared from various concentrations of collagen solutions, and their effect on the regeneration of tracheal epithelium was examined. Methods: Collagen sponges were prepared from type I and III collagen solutions. The structures of the sponges were analyzed using scanning electron microscopy (SEM). Artificial tracheae, which were formed using the collagen sponges with different structures, were implanted into rabbits, and regeneration of the tracheal epithelium on the artificial tracheae was evaluated by SEM analysis and histological examination. Results: The SEM analysis showed that collagen sponges prepared from 0.5% and 1.0% collagen solutions had a porous structure. However, the sponges prepared from a 1.5% collagen solution had a nonporous structure. After implantation of artificial tracheae prepared from 0.5% and 1.0% collagen solutions, their luminal surfaces were mostly covered with epithelium within 14 days. However, epithelial reorganization occurred later on artificial tracheae prepared from the 1.5% collagen solution. Conclusion: Collagen sponges with a porous structure are suitable for regeneration of the tracheal epithelium in our artificial trachea. [ABSTRACT FROM AUTHOR]

Published
2016
Full Text
View/download PDF

230. Sterilized, freeze-dried amniotic membrane : a useful substrate for ocular surface reconstruction.

Author

Nakamura, Takahiro, Yoshitani, Makoto, Rigby, Helen, Fullwood, Nigel J., Ito, Wakana, Inatomi, Tsutomu, Sotozono, Chie, Nakamura, Tatsuo, Shimizu, Yasuhiko, Kinoshita, Shigeru, Nakamura, Takahiro, Yoshitani, Makoto, Rigby, Helen, Fullwood, Nigel J., Ito, Wakana, Inatomi, Tsutomu, Sotozono, Chie, Nakamura, Tatsuo, Shimizu, Yasuhiko, and Kinoshita, Shigeru

Abstract

PURPOSE. To examine the feasibility of using sterilized, freeze-dried amniotic membrane (FD-AM) as a substrate for cultivating autologous corneal epithelial cells for ocular surface reconstruction. METHODS. Human AM deprived of amniotic epithelial cells by incubation with EDTA was freeze dried, vacuum packed, and sterilized with -irradiation. The resultant FD-AM was characterized for its physical, biological, and morphologic properties by stretch stress tests, immunohistochemistry, electron microscopy, and cell culture. In addition, 3 weeks after an ocular surface injury, the conjunctivalized corneal surfaces of eyes in eight rabbits were surgically reconstructed by transplantation of autologous cultivated corneal epithelial cells on FD-AM. RESULTS. A stretch stress test revealed no significant differences between sterilized FD-AM and cryopreserved AM. Immunohistochemistry for several extracellular matrix molecules and electron microscopic analysis of FD-AM revealed that the process of drying and irradiation did not affect its biological and morphologic properties. The corneal epithelial cells cultivated on FD-AM had four to five stratified, well-differentiated cell layers. Corneas that were grafted with the cultivated corneal epithelial cells on FD-AM were clear and were all epithelialized at 10 days after surgery. CONCLUSIONS. The sterilized, freeze-dried AM retained most of the physical, biological, and morphologic characteristics of cryopreserved AM; consequently, it is a useful biomaterial for ocular surface reconstruction.

Published
2004

Catalog

Books, media, physical & digital resources
See catalog results