654 results on '"Muramatsu, Masamichi"'
Search Results
202. Aid
- Author
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Honjo, Tasuku, primary, Muramatsu, Masamichi, additional, and Fagarasan, Sidonia, additional
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- 2004
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203. AID mutant analyses indicate requirement for class-switch-specific cofactors
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Ta, Van-Thanh, primary, Nagaoka, Hitoshi, additional, Catalan, Nadia, additional, Durandy, Anne, additional, Fischer, Alain, additional, Imai, Kohsuke, additional, Nonoyama, Shigeaki, additional, Tashiro, Junko, additional, Ikegawa, Masaya, additional, Ito, Satomi, additional, Kinoshita, Kazuo, additional, Muramatsu, Masamichi, additional, and Honjo, Tasuku, additional
- Published
- 2003
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204. Unmutated Immunoglobulin M Can Protect Mice from Death by Influenza Virus Infection
- Author
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Harada, Yuichi, primary, Muramatsu, Masamichi, additional, Shibata, Toshikatsu, additional, Honjo, Tasuku, additional, and Kuroda, Kazumichi, additional
- Published
- 2003
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205. Constitutive Expression of AID Leads to Tumorigenesis
- Author
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Okazaki, Il-mi, primary, Hiai, Hiroshi, additional, Kakazu, Naoki, additional, Yamada, Shuichi, additional, Muramatsu, Masamichi, additional, Kinoshita, Kazuo, additional, and Honjo, Tasuku, additional
- Published
- 2003
- Full Text
- View/download PDF
206. DNA Double-Strand Breaks
- Author
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Bross, Linda, primary, Muramatsu, Masamichi, additional, Kinoshita, Kazuo, additional, Honjo, Tasuku, additional, and Jacobs, Heinz, additional
- Published
- 2002
- Full Text
- View/download PDF
207. Molecular Mechanism of Class Switch Recombination: Linkage with Somatic Hypermutation
- Author
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Honjo, Tasuku, primary, Kinoshita, Kazuo, additional, and Muramatsu, Masamichi, additional
- Published
- 2002
- Full Text
- View/download PDF
208. Activation-induced Deaminase (AID)-directed Hypermutation in the Immunoglobulin Sμ Region
- Author
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Nagaoka, Hitoshi, primary, Muramatsu, Masamichi, additional, Yamamura, Namiko, additional, Kinoshita, Kazuo, additional, and Honjo, Tasuku, additional
- Published
- 2002
- Full Text
- View/download PDF
209. Class Switch Recombination and Hypermutation Require Activation-Induced Cytidine Deaminase (AID), a Potential RNA Editing Enzyme
- Author
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Muramatsu, Masamichi, primary, Kinoshita, Kazuo, additional, Fagarasan, Sidonia, additional, Yamada, Shuichi, additional, Shinkai, Yoichi, additional, and Honjo, Tasuku, additional
- Published
- 2000
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210. Isolation, Tissue Distribution, and Chromosomal Localization of the Human Activation-Induced Cytidine Deaminase (AID) Gene
- Author
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Muto, Taro, primary, Muramatsu, Masamichi, additional, Taniwaki, Masafumi, additional, Kinoshita, Kazuo, additional, and Honjo, Tasuku, additional
- Published
- 2000
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- View/download PDF
211. Low-affinity IgM antibodies lacking somatic hypermutations are produced in the secondary response of C57BL/6 mice to (4-hydroxy-3-nitrophenyl)acetyl hapten.
- Author
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Murakami, Akikazu, Moriyama, Hayato, Osako-Kabasawa, Mina, Endo, Kanako, Nishimura, Miyuki, Udaka, Keiko, Muramatsu, Masamichi, Honjo, Tasuku, Azuma, Takachika, and Shimizu, Takeyuki
- Subjects
IMMUNOGLOBULIN M ,SOMATIC mutation ,IMMUNE response ,B cells ,ENZYME-linked immunosorbent assay ,NITROPHENYL compounds ,LABORATORY mice - Abstract
Low-affinity IgM antibodies feature in secondary immune responsesClass-switched memory B cells, which are generated through the processes of somatic hypermutation (SHM) and affinity-based selection in germinal centers, contribute to the production of affinity-matured IgG antibodies in the secondary immune response. However, changes in the affinity of IgM antibodies during the immune response have not yet been studied, although IgM+ memory B cells have been shown to be generated. In order to understand the relationship between IgM affinity and the recall immune response, we prepared hybridomas producing anti-(4-hydroxy-3-nitrophenyl)acetyl (NP) IgM antibodies from C57BL/6 mice and from activation-induced cytidine deaminase (AID)-deficient mice. Binding analysis by ELISA showed that mAbs obtained from the secondary immune response contained IgM mAbs with affinity lower than the affinity of mAbs obtained from the primary response. By analyzing sequences of the IgM genes of hybridomas and plasma cells, we found many unmutated VH genes. VH genes that had neither tyrosine nor glycine at position 95 were frequent. The repertoire change may correlate with the lower affinity of IgM antibodies in the secondary response. The sequence and affinity changes in IgM antibodies were shown to be independent of SHM by analyzing hybridomas from AID-deficient mice. A functional assay revealed a reciprocal relationship between affinity and complement-dependent hemolytic activity toward NP-conjugated sheep RBCs; IgM antibodies with lower affinities had higher hemolytic activity. These findings indicate that lower affinity IgM antibodies with enhanced complement activation function are produced in the secondary immune response. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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- View/download PDF
212. Specific Expression of Activation-induced Cytidine Deaminase (AID), a Novel Member of the RNA-editing Deaminase Family in Germinal Center B Cells
- Author
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Muramatsu, Masamichi, primary, Sankaranand, V.S., additional, Anant, Shrikant, additional, Sugai, Manabu, additional, Kinoshita, Kazuo, additional, Davidson, Nicholas O., additional, and Honjo, Tasuku, additional
- Published
- 1999
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213. Fractalkine and macrophage-derived chemokine: T cell-attracting chemokines expressed in T cell area dendritic cells
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Kanazawa, Nobuo, primary, Nakamura, Tomoyuki, additional, Tashiro, Kei, additional, Muramatsu, Masamichi, additional, Morita, Kazumasa, additional, Yoneda, Kozo, additional, Inaba, Kayo, additional, Imamura, Sadao, additional, and Honjo, Tasuku, additional
- Published
- 1999
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214. Inhibition of Carnitine Palmitoyltransferase I Augments Sphingolipid Synthesis and Palmitate-induced Apoptosis
- Author
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Paumen, Michael B., primary, Ishida, Yasumasa, additional, Muramatsu, Masamichi, additional, Yamamoto, Masaharu, additional, and Honjo, Tasuku, additional
- Published
- 1997
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215. Direct Interaction of the Mitochondrial Membrane Protein Carnitine Palmitoyltransferase I with Bcl-2
- Author
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Paumen, Michael B., primary, Ishida, Yasumasa, additional, Han, Hua, additional, Muramatsu, Masamichi, additional, Eguchi, Yutaka, additional, Tsujimoto, Yoshihide, additional, and Honjo, Tasuku, additional
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- 1997
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216. Experimental Cross-Species Transmission of Rat Hepatitis E Virus to Rhesus and Cynomolgus Monkeys.
- Author
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Yang, Fengmei, Li, Yanyan, Li, Yongjie, Jin, Weihua, Duan, Suqin, Xu, Hongjie, Zhao, Yuan, He, Zhanlong, Ami, Yasushi, Suzaki, Yuriko, Doan, Yen Hai, Takeda, Naokazu, Zhang, Wenjing, Muramatsu, Masamichi, and Li, Tian-Cheng
- Subjects
ALANINE aminotransferase ,HEPATITIS E virus ,KRA ,RHESUS monkeys ,RATS ,SIMIAN viruses ,SPRAGUE Dawley rats - Abstract
Rat hepatitis E virus (rat HEV) was first identified in wild rats and was classified as the species Orthohepevirus C in the genera Orthohepevirus, which is genetically different from the genotypes HEV-1 to HEV-8, which are classified as the species Orthohepevirus A. Although recent reports suggest that rat HEV transmits to humans and causes hepatitis, the infectivity of rat HEV to non-human primates such as cynomolgus and rhesus monkeys remains controversial. To investigate whether rat HEV infects non-human primates, we inoculated one cynomolgus monkey and five rhesus monkeys with a V-105 strain of rat HEV via an intravenous injection. Although no significant elevation of alanine aminotransferase (ALT) was observed, rat HEV RNA was detected in fecal specimens, and seroconversion was observed in all six monkeys. The partial nucleotide sequences of the rat HEV recovered from the rat HEV-infected monkeys were identical to those of the V-105 strain, indicating that the infection was caused by the rat HEV. The rat HEV recovered from the cynomolgus and rhesus monkeys successfully infected both nude and Sprague-Dawley rats. The entire rat HEV genome recovered from nude rats was identical to that of the V-105 strain, suggesting that the rat HEV replicates in monkeys and infectious viruses were released into the fecal specimens. These results demonstrated that cynomolgus and rhesus monkeys are susceptible to rat HEV, and they indicate the possibility of a zoonotic infection of rat HEV. Cynomolgus and rhesus monkeys might be useful as animal models for vaccine development. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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217. RNA editing of hepatitis B virus transcripts by activation-induced cytidine deaminase.
- Author
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Guoxin Liang, Kitamura, Kouichi, Zhe Wang, Guangyan Liu, Chowdhury, Sajeda, Weixin Fu, Koura, Miki, Wakae, Kousho, Honjo, Tasuku, and Muramatsu, Masamichi
- Subjects
RNA editing ,HEPATITIS B virus ,CYTIDINE deaminase ,SOMATIC mutation ,DEAMINATION ,URIDINE ,NUCLEOCAPSIDS ,NUCLEOPROTEINS - Abstract
Activation-induced cytidine deaminase (AID) is essential for the somatic hypermutation (SHM) and class-switch recombination (CSR) of lg genes. The mechanism by which AID triggers SHM and (SR has been explained by two distinct models. In the DNA deamination model, AID converts cytidine bases in DNA into uridine. The uridine is recognized by the DNA repair system, which produces DNA strand breakages and point mutations. In the alternative model, RNA edi- ted by AID is responsible for triggering (SR and SHM. However, RNA deamination by AID has not been demonstrated. Here we found that C-to-T and 6-to-A mutations accumulated in hepatitis B virus (HBV) nudeocapsid DNA when AID was expressed in HBV-replicating hepatic cell lines. AID expression caused C-to-T mutations in the nucleocapsid DNA of RNase H-defective HBV. which does not produce plus-strand viral DNA. Furthermore, the RT-P(R products of nucleocapsid viral RNA from AID-expressing cells exhibited significant C-to-T mutations, whereas viral RNAs outside the nucleocapsid did not accumulate C-to-U mutations. Moreover, AID was packaged within the nucleocapsid by forming a ribonucleo-protein complex with HBV RNA and the HBV polymerase protein. The encapsidation of the AID protein with viral RNA and DNA provides an efficient environment for evaluating AID's RNA and DNA deamination activities. A bona fide RNA-editing enzyme, apolipoprotein B mRNA editing catalytic polypeptide 1, induced a similar level of C-to-U mutations in nucleocapsid RNA as AID. Taken together, the results indicate that AID can deaminate the nucleocapsid RNA of HBV. [ABSTRACT FROM AUTHOR]
- Published
- 2013
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218. Histone3 lysine4 trimethylation regulated by the facilitates chromatin transcription complex is critical for DNA cleavage in class switch recombination.
- Author
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Stanhie, Andre, Aida, Masatoshi, Muramatsu, Masamichi, Honjo, Tasuku, and Begum, Nasim A.
- Subjects
HISTONES ,BASIC proteins ,CHROMATIN ,NUCLEOPROTEINS ,LYSINE - Abstract
Ig class switch recombination (CSR) requires expression of activation-induced cytidine deaminase (AID) and transcription through target switch (S) regions. Here we show that knockdown of the histone chaperone facilitates chromatin transcription (FACT) completely inhibited S region cleavage and CSR in IgA-switch-inducible CH12F3-2A B cells. FACT knockdown did not reduce AID or S region transcripts but did decrease histone3 lysine4 trimethylation (H3K4me3) at both the Sμ and Sα regions. Because knockdown of FACT or H3K4 methyltransferase cofactors inhibited DNA cleavage in H3K4me3-depleted S regions, H3K4me3 may serve as a mark for recruiting CSR recombinase. These findings revealed an un- expected evolutionary conservation between CSR and meiotic recombination. [ABSTRACT FROM AUTHOR]
- Published
- 2010
- Full Text
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219. AID-induced decrease in topoisomerase 1 induces DNA structural alteration and DNA cleavage for class switch recombination.
- Author
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Kobayashi, Maki, Aida, Masatoshi, Nagaoka, Hitoshi, Begum, Nasim A., Kitawaki, Yoko, Nakata, Mikiyo, Stanlie, Andre, Doi, Tomomitsu, Kato, Lucia, Okazaki, Il-mi, Shinkura, Reiko, Muramatsu, Masamichi, Kinoshita, Kazuo, and Honjo, Tasuku
- Subjects
CAMPTOTHECIN ,GENETIC recombination ,DNA topoisomerase I ,ADENOSINE deaminase ,GENETIC transformation - Abstract
To initiate class switch recombination (CSR) activation-induced cytidine deaminase (AID) induces staggered nick cleavage in the S region, which lies 5' to each Ig constant region gene and is rich in palindromic sequences. Topoisomerase 1 (Top1) controls the supercoiling of DNA by nicking, rotating, and religating one strand of DNA. Curiously, Topi reduction or AID overexpression causes the genomic instability. Here, we report that the inactivation of Topi by its specific inhibitor camptothecin drastically blocked both the S region cleavage and CSR, indicating that Topi is responsible for the S region cleavage in CSR. Surprisingly, AID expression suppressed Topi mRNA translation and reduced its protein level. In addition, the decrease in the Topi protein by RNA-mediated knockdown augmented the AID-dependent S region cleavage, as well as CSR. Furthermore, Topi reduction altered DNA structure of the S region. Taken together, AID-induced Topi reduction alters S region DNA structure probably to non-B form, on which Topi can introduce nicks but cannot religate, resulting in S region cleavage. [ABSTRACT FROM AUTHOR]
- Published
- 2009
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220. Carboxy-terminal domain of AID required for its mRNA complex formation in vivo.
- Author
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Nonaka, Taichiro, Doi, Tomomitsu, Toyoshima, Takae, Muramatsu, Masamichi, Honjo, Tasuku, and Kinoshita, Kazuo
- Subjects
IMMUNOGLOBULIN genes ,IR genes ,HOMOLOGY (Biology) ,COMPARATIVE anatomy ,RNA ,NUCLEIC acids - Abstract
Activation-induced cytidine deaminase (AID) is essential for the class switch recombination (CSR) and somatic hypermutation (SHM) of Ig genes. Originally, AID was postulated to be an RNA- editing enzyme, because of its structural homology with a known RNA-editing enzyme, APOBEC1. In support of this idea, AID shares many of the properties of RNA-editing enzymes, including nude- ocytoplasmic shuttling and a dependency on de novo protein synthesis. However, it has not been shown whether AID recognizes a specific mRNA and edits it to generate an enzyme involved in CSR or SHM. Here, we examined the association between AID and polyadenylated [poly(A)
+ ] RNA in vivo, using UV cross-linking coupled with a poly(A) capture method that relies on biotinylated oligo(dT) and streptavidin-conjugated beads. We found that both exogenous AID expressed in transfected CH12 cells and endogenous AID expressed in BL2 cells were associated with poIy(A)+ RNA. Similar protein-poly(A)+ RNA complexes were formed by APOBEC1 and APOBEC3G. However, the interactions of all of these cytidine deaminase family members, including AID, with poly(A)+ RNA were indirect. This was expected for APOBEC1, which is known to act through an RNA-interacting cofactor, APOBEC1 complementation factor (ACF). In addition, the carboxy-terminal region of AID, which is essential for class switching, was also required for its interaction with poly(A)+ RNA. These results suggest that the CSR activity of AID requires an ACF-like cofactor that specifically interacts with the carboxy-terminal domain of AID. [ABSTRACT FROM AUTHOR]- Published
- 2009
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221. A Cross-Species Transmission of a Camel-Derived Genotype 8 Hepatitis E Virus to Rabbits.
- Author
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Zhang, Wenjing, Ami, Yasushi, Suzaki, Yuriko, Kataoka, Michiyo, Takeda, Naokazu, Muramatsu, Masamichi, and Li, Tiancheng
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HEPATITIS E virus ,RABBITS ,WILD boar ,ALANINE aminotransferase ,KRA ,VIRUS-like particles ,IMMUNOGLOBULIN G ,GENOTYPES - Abstract
Novel genotypes of hepatitis E virus (HEV), i.e., HEV-5, HEV-7, and HEV-8, have been identified in wild boar, dromedary camels, and Bactrian camels, respectively, and they transmit to cynomolgus monkeys in a trans-species manner, raising the potential for zoonotic infection. Rabbits are the natural reservoir for rabbit HEV, but they are also susceptible to HEV-3 and HEV-4. It has been unknown whether rabbits are susceptible to HEV-5, HEV-7, and HEV-8. To investigate the infectivity of novel HEVs in rabbits and to assess whether rabbits are appropriate animal models for these HEVs, we inoculated Japanese white rabbits with HEV-5, HEV-7, and HEV-8, respectively. We observed that viral RNA was present in the fecal specimens of the HEV-8-inoculated rabbits and anti-HEV IgG antibodies were present in its sera, although anti-HEV IgM was undetectable and no significant elevation of ALT was observed. These results indicated that HEV-8 crossed species and infected the rabbits. No evidence for replication was observed in HEV-5 and HEV-7, suggesting that rabbits are not susceptible to these genotypes. The antibodies elicited in the HEV-8-infected rabbits did not protect them from the rabbit HEV challenge, suggesting that the antigenicity differs between HEV-8 and rabbit HEV. Antigenic analyses demonstrated that anti-HEV-8 antibodies reacted more strongly with homologous HEV-8 virus-like particles (VLPs) compared to heterologous rabbit HEV VLPs, but anti-rabbit HEV antibody had similar reactivity to the VLPs of rabbit HEV and HEV-8, suggesting that HEV-8 lacks some epitope(s) that exist in rabbit HEV and induced the neutralizing antibodies against rabbit HEV. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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222. AID is required for germinal center–derived lymphomagenesis.
- Author
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Pasqualucci, Laura, Bhagat, Govind, Jankovic, Mila, Compagno, Mara, Smith, Paula, Muramatsu, Masamichi, Honjo, Tasuku, Morse III, Herbert C., Nussenzweig, Michel C., and Dalla-Favera, Riccardo
- Subjects
LYMPHOMAS ,CARCINOGENESIS ,GENETIC recombination ,B cells ,MEDICAL genetics - Abstract
Most human B cell non-Hodgkin's lymphomas (B-NHLs) derive from germinal centers (GCs), the structure in which B cells undergo somatic hypermutation (SHM) and class switch recombination (CSR) before being selected for high-affinity antibody production. The pathogenesis of B-NHL is associated with distinct genetic lesions, including chromosomal translocations and aberrant SHM, which arise from mistakes occurring during CSR and SHM. A direct link between these DNA remodeling events and GC lymphoma development, however, has not been demonstrated. Here we have crossed three mouse models of B cell lymphoma driven by oncogenes (Myc, Bcl6 and Myc/Bcl6; refs. 5,6) with mice lacking activation-induced cytidine deaminase (AID), the enzyme required for both CSR and SHM. We show that AID deficiency prevents Bcl6-dependent, GC-derived B-NHL, but has no impact on Myc-driven, pre-GC lymphomas. Accordingly, abrogation of AID is associated with the disappearance of CSR- and SHM-mediated structural alterations. These results show that AID is required for GC-derived lymphomagenesis, supporting the notion that errors in AID-mediated antigen-receptor gene modification processes are principal contributors to the pathogenesis of human B-NHL. [ABSTRACT FROM AUTHOR]
- Published
- 2008
- Full Text
- View/download PDF
223. Activation-induced cytidine deaminase (AID) promotes B cell lymphomagenesis in Emu-cmyc transgenic mice.
- Author
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Kotani, Ai, Kakazu, Naoki, Tsuruyama, Tatsuaki, Okazaki, Il-mi, Muramatsu, Masamichi, Kinoshita, Kazuo, Nagaoka, Hitoshi, Yabe, Daisuke, and Honjo, Tasuku
- Subjects
B cells ,LYMPHOMAS ,TRANSGENIC mice ,LEUKEMIA ,DNA ,RETICULOENDOTHELIAL granulomas - Abstract
Activation-induced cytidine deaminase (AID), which is essential to both class switch recombination and somatic hypermutation of the Ig gene, is expressed in many types of human B cell lymphoma/ leukemia. AID is a potent mutator because it is involved in DNA breakage not only of Ig but also of other genes, including proto-oncogenes. Recent studies suggest that AID is required for chromosomal translocation involving cmyc and Ig loci. However, it is unclear whether AID plays other roles in tumorigenesis. We examined the effect of AID deficiency on the generation of surface 19-positive B cell lymphomas in Emu-cmyc transgenic mice. Almost all lymphomas that developed in AID-deficient transgenic mice were pre-B cell lymphomas, whereas control transgenic mice had predominantly B cell lymphomas, indicating that AID is required for development of B but not pre-B cell lymphomas from cmyc over-expressing tumor progenitors. Thus, AID may play multiple roles in B cell lymphomagenesis. [ABSTRACT FROM AUTHOR]
- Published
- 2007
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224. Negative regulation of activation-induced cytidine deaminase in B cells.
- Author
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Muto, Taro, Okazaki, Il-mi, Yamada, Shuichi, Tanaka, Yoshimasa, Kinoshita, Kazuo, Muramatsu, Masamichi, Nagaoka, Hitoshi, and Tasuku Honjo
- Subjects
ADENOSINE deaminase ,B cells ,LYMPHOCYTES ,LYMPHOID tissue ,CYTIDINE diphosphate choline ,ANTIGEN presenting cells - Abstract
Both class switch recombination (CSR) and somatic hypermutation (SHM) of the Ig genes require the activity of activation-induced cytidine deaminase (AID). Expression of AID is restricted to B cells in the germinal centers of the lymphoid organs, where activated B cells undergo CSR and SHM. We previously showed that constitutive and systemic expression of AID leads to tumorigenesis in T cells and lung epithelium, but not in B cells. This finding led us to suspect that transgenic AID may be inactivated at least in part in B cells. To address this issue, we generated conditional AID-transgenic mice that constitutively express AID only in B cells. Studies on the cross between the AID-transgenic and AID-deficient mice showed that abundant AID protein accumulated by constitutive expression is inactivated in B cells, possibly providing an explanation for the absence of deregulation of CSR and SHM in AID-transgenic B cells. [ABSTRACT FROM AUTHOR]
- Published
- 2006
- Full Text
- View/download PDF
225. A target selection of somatic hypermutations is regulated similarly between T and B cells upon activation-induced cytidine deaminase expression.
- Author
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Kotani, Ai, Okazaki, Il-mi, Muramatsu, Masamichi, Kinoshita, Kazuo, Begum, Nasim A., Nakajima, Toshiharu, Saito, Hirohisa, and Honjo, Tasuku
- Subjects
B cells ,LYMPHOMAS ,LYMPHOCYTES ,LYMPHOPROLIFERATIVE disorders ,T cells ,CELL membranes ,TUMOR suppressor genes - Abstract
Activation-induced cytidine deaminase (AID) is essential for somatic hypermutations (SMM) and class switch recombination. Overexpression of AID in non-B cells can induce SHM in artificial constructs inserted in various loci in the genome. AID overexpression was thus proposed to introduce mutations in a wide variety of genes with little specificity. We previously showed that AID transgenic mice developed T cell lymphomas in which the variable region β genes of the T cell receptor and c-myc were mutated as frequently as SHM in activated B cells. To understand the target specificity of SHM in AID-expressing T lymphomas, we sequenced six oncogenes (c-myc, pim1, p53, atm, tgfbr-2, and k-ras) and two genes (cd4 and cds) that are actively transcribed in T lymphomas. SHM was found only in c-myc, pim1, cd4, and cds, which share the E47 binding motif in the enhancer/promoter. The rest that are not mutated in B cells were not mutated in AID-induced T lymphomas either, although they are transcribed in T and B cells. Comparison of several features of SHM, including selection of targets and mutation distribution, suggests that the regulatory mechanism of 5MM is similar between T and B cells. 5MM base specificities in the CD4 and CD5 genes were biased to AT, indicating that the preference of target bases of the mutations generated by overexpression of AID is not always GC bases but variable between target genes. [ABSTRACT FROM AUTHOR]
- Published
- 2005
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- View/download PDF
226. DNA cleavage in immunoglobulin somatic hypermutation depends on de novo protein synthesis but not on uracil DNA glycosylase.
- Author
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Nagaoka, Hitoshi, Ito, Satomi, Muramatsu, Masamichi, Nakata, Mikiyo, and Honjo, Tasuku
- Subjects
DNA ,IMMUNOGLOBULINS ,URACIL ,MESSENGER RNA ,PROTEIN synthesis ,NUCLEIC acids - Abstract
Activation-induced cytidine deaminase (AID) is required for the DNA cleavage step of Ig somatic hypermutation (SHM). However, its molecular mechanism is controversial. The RNA editing hypothesis postulates that AID deaminates cytosine in an unknown mRNA to generate a new mRNA encoding SHM endonuclease. On the other hand, the DNA deamination hypothesis explains DNA cleavage by cytosine deamination in DNA, followed by uracil removal by uracil DNA glycosylase (UNG). By using the protein synthesis inhibitor cycloheximide, we showed that SHM requires tie novo protein synthesis in accord with predictions by the RNA editing hypothesis. In addition, we found that cycloheximide but not Ugi (the specific inhibitor of UNG) inhibited AID-dependent DNA cleavage in the Ig gene during SHM, by using histone H2AX focus formation as a marker of DNA cleavage. The results indicate the following order of events: AID expression, protein synthesis, DNA cleavage, and SHM. The requirement of protein synthesis but not of UNG for the DNA cleavage step of SHM forces us to reconsider the DNA deamination hypothesis and strengthens the RNA editing hypothesis. [ABSTRACT FROM AUTHOR]
- Published
- 2005
- Full Text
- View/download PDF
227. IFN-γ‒Induced APOBEC3B Contributes to Merkel Cell Polyomavirus Genome Mutagenesis in Merkel Cell Carcinoma
- Author
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Que, Lusheng, Li, Yingfang, Dainichi, Teruki, Kukimoto, Iwao, Nishiyama, Tomoaki, Nakano, Yuri, Shima, Kaori, Suzuki, Tadaki, Sato, Yuko, Horike, Shinichi, Aizaki, Hideki, Watashi, Koichi, Kato, Takanobu, Aly, Hussein H., Watanabe, Noriyuki, Kabashima, Kenji, Wakae, Kousho, and Muramatsu, Masamichi
- Abstract
Merkel cell polyomavirus (MCPyV) is the causative agent of an aggressive skin tumor, Merkel cell carcinoma. The viral genome is integrated into the tumor genome and harbors nonsense mutations in the helicase domain of large T antigen. However, the molecular mechanisms by which the viral genome gains the tumor-specific mutations remain to be elucidated. Focusing on host cytosine deaminases APOBEC3s, we find that A3A, A3B, or A3G introduces A3-specific mutations into episomal MCPyV genomes in MCPyV-replicating 293-derivative cells. Sequence analysis of MCPyV genomes retrieved from the NCBI database revealed a decrease of TpC dinucleotide, a preferred target for A3A and A3B, in the 3′-region of the large T antigen‒coding sequence. The viral DNA isolated from tumors contained mutated cytosines, with a remarkable bias toward TpC dinucleotide. Analysis of publicly available microarray data showed that expression of IFN-γ and cytotoxic T lymphocyte markers was positively correlated with the A3A, A3B, and A3G levels in MCPyV-positive but not in MCPyV-negative tumors. Finally, IFN-γ treatment induced A3B and A3G expression in the MCPyV-positive Merkel cell carcinoma cell line MS-1. These results suggest that the IFN-γ–A3B axis plays pivotal roles in evolutionally shaping MCPyV genomic sequences and in generating tumor-specific large T antigen mutations during development of Merkel cell carcinoma.
- Published
- 2022
- Full Text
- View/download PDF
228. De novo protein synthesis is required for activation- induced cytidine deaminase-dependent DNA cleavage in immunoglobulin class switch recombination.
- Author
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Begum, Nasim A., Kinoshita, Kazuo, Muramatsu, Masamichi, Nagaoka, Hitoshi, Shinkura, Reiko, and Tasuku Honjo
- Subjects
PROTEIN synthesis ,IMMUNOGLOBULINS ,MESSENGER RNA ,ENDONUCLEASES ,DNA ,CHROMATIN - Abstract
Activation-induced cytidine deaminase is required for the DNA cleavage step of Ig class switch recombination (CSR). However, its molecular mechanism is controversial. RNA-editing hypothesis postulates that activation-induced cytidine deaminase deaminates cytosine in an unknown mRNA to generate a new mRNA encoding an endonuclease for CSR and thus predicts that DNA cleavage depends on de nova protein synthesis. On the other hand, DNA deamination hypothesis proposes that DNA cleavage is initiated by cytosine deamination in DNA, followed by uracil removal by uracil DNA glycosylase. By using the chromatin immunoprecipitation assay to detect γ-H2AX focus formation as a marker for DNA cleavage, we found that cycloheximide inhibited DNA cleavage in the Ig heavy-chain locus during CSR. Requirement of protein synthesis in the DNA cleavage step of CSR strengthens the RNA- editing hypothesis. [ABSTRACT FROM AUTHOR]
- Published
- 2004
- Full Text
- View/download PDF
229. Separate domains of AID are required for somatic hypermutation and class-switch recombination.
- Author
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Shinkura, Reiko, Ito, Satomi, Begum, Nasim A., Nagaoka, Hitoshi, Muramatsu, Masamichi, Kinoshita, Kazuo, Sakakibara, Yoshimasa, Hijikata, Hiroko, and Tasuku Honjo
- Subjects
GENETIC mutation ,B cells ,GENETIC recombination ,BIOLOGICAL variation ,LYMPHOCYTES ,GENETICS - Abstract
Activation-induced cytidine deaminase (AID) is essential for class-switch recombination (CSR) and somatic hypermutation (SHM). Mutants with changes in the C-terminal region of AID retain SHM but lose CSR activity. Here we describe five mutants with alterations in the N-terminal region of AID that caused selective deficiency in SHM but retained CSR, suggesting that the CSR and SHM activities of AID may dissociate via interaction of CSR- or SHM-specific cofactors with different domains of AID. Unlike cells expressing C-terminal AID mutants, B cells expressing N-terminal AID mutants had mutations in the switch µ region, indicating that such mutations are generated by reactions involved in CSR but not SHM. Thus, we propose that separate domains of AID interact with specific cofactors to regulate these two distinct genetic events in a target-specific way. [ABSTRACT FROM AUTHOR]
- Published
- 2004
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230. Unmutated immunoglobulin M can protect mice from death by influenza virus infection
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Harada, Yuichi, Muramatsu, Masamichi, Shibata, Toshikatsu, Honjo, Tasuku, Shimizu, Kazufumi, Matsumura, Eiko, and Kuroda, Kazumichi
- Subjects
- *
INFLUENZA viruses , *MONOCLONAL antibodies , *IMMUNOGLOBULINS , *MICE - Abstract
To elucidate the role of class switch recombination (CSR) and somatic hypermutation (SHM) in virus infection, we have investigated the influence of the infection of influenza virus on mice deficient of activation-induced cytidine deaminase (AID), which is absolutely required for CSR and SHM. In the primary infection, mortality of AID−/− mice was similar to that of AID+/− mice. AID−/− mice, however, showed severer morbidity and delayed elimination of the virus than AID+/− mice, while virus neutralizing antibody titers were similar between two genotypes. In the secondary infection with a lethal dose of influenza virus only AID−/− mice permitted vigorous viral replication and lost their weights severely, although both genotypes of mice survived completely. The survival of AID−/− mice was not affected by depletion of CD8+ T cells brought about by administration of an anti-CD8 monoclonal antibody. These results indicate that unmutated IgM alone is capable of protecting mice from death upon primary and secondary infections, and severer morbidity in AID−/− mice should be due to the absence of either other immunoglobulin classes such as IgG, high affinity antibodies with SHM or both. [Copyright &y& Elsevier]
- Published
- 2004
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231. RNA-editing cytidine deaminase Apobec-1 is unable to induce somatic hypermutation in mammalian cells.
- Author
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Eto, Tomonori, Kinoshita, Kazuo, Yoshikawa, Kiyotsugu, Muramatsu, Masamichi, and Honjo, Tasuku
- Subjects
GENETIC mutation ,ESCHERICHIA coli ,RNA editing ,ANTIBODY diversity - Abstract
Antibody diversification by somatic hypermutation, gene conversion, and class switch recombination is completely dependent on activation-induced cytidine deaminase (AID). A recent report showing induction of DNA mutations in Escherichia coli by overexpression of AID, Apobec-1, and related members of the RNA-editing cytidine deaminase family suggested that they may directly modify deoxycytidine in DNA in mammalian cells (DNA-editing model). We therefore examined whether Apobec-1 bona fide RNA-editing enzyme could show somatic hypermutation and class switching activities in murine B lymphocytes and fibroblasts. Unlike AID, Apobec-1 was unable to induce somatic hypermutation or class switching. The results force a reevaluation of the physiological significance of the DNA deaminase activities of AID and Apobec-1 in E. coli and in vitro. [ABSTRACT FROM AUTHOR]
- Published
- 2003
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232. De novo protein synthesis is required for the activation-indiced cytidine deaminase function in class-switch recombination.
- Author
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Doi, Tomomitsu, Kinoshita, Kazuo, Ikegawa, Masaya, Muramatsu, Masamichi, and Honjo, Tasuku
- Subjects
ADENOSINE deaminase ,IMMUNOGLOBULIN genes - Abstract
Activation-induced cytidine deaminase (AID) is required for classswitch recombination (CSR), somatic hypermutation, and gene conversion of Ig genes. Although AID has sequence similarity to an RNA-editing enzyme Apobec-1, how AID functions in CSR and somatic hypermutation is unknown. Because involvement of RNAediting but not DNA-editing in CSR requires de novo protein synthesis after AID expression, we examined whether protein synthesis inhibitors could block CSR in the presence of the AID activity. For this purpose we constructed AID fused with the hormone-binding domain of the estrogen receptor (AID-ER), which was introduced into AID-deficient spleen B cells. When such transfectants were treated with an estrogen analogue, 4-hydroxytamoxifen (OHT), CSR was induced within 1 h. Cycloheximide or puromycin drastically suppressed OHT-induced CSR in AID-ER expressing AID[sup -/-] B cells when added I h before OHT but not after OHT, suggesting that de novo protein synthesis is required for an event downstream to AID expression in CSR. The results lend the weight to RNA-editing hypothesis for the function of AID. [ABSTRACT FROM AUTHOR]
- Published
- 2003
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233. Critical Roles of Activation-Induced Cytidine Deaminase in the Homeostasis of Gut Flora.
- Author
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Fagarasan, Sidonia, Muramatsu, Masamichi, Suzuki, Keiichiro, Nagaoka, Hitoshi, Hiai, Hiroshi, and Honjo, Tasuku
- Subjects
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ENZYMES , *GENETIC recombination , *GENETIC mutation , *IMMUNOGLOBULINS , *HOMEOSTASIS - Abstract
Activation-induced cytidine deaminase (AID) plays an essential role in class switch recombination (CSR) and somatic hypermutation (SHM) of immunoglobulin genes. We report here that deficiency in AID results in the development of hyperplasia of isolated lymphoid follicles (ILFs) associated with a 100-fold expansion of anaerobic flora in the small intestine. Reduction of bacterial flora by antibiotic treatment of AID[sup -/-] mice abolished ILF hyperplasia as well as the germinal center enlargement seen in secondary lymphoid tissues. Because an inability to switch to immunoglobulin A on its own does not lead to a similar phenotype, these results suggest that SHM of ILF B cells plays a critical role in regulating intestinal microftora. [ABSTRACT FROM AUTHOR]
- Published
- 2002
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234. AID is required to initiate Nbs1/gamma-H2AX focus formation and mutations at sites of class switching.
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Peterson, Simone, Casellas, Rafael, Reina-San-Martin, Bernardo, Hua Tang Chen, Difilippantonio, Michael J., Wilson, Patrick C., Hanitsch, Leif, Celeste, Arkady, Muramatsu, Masamichi, Pilch, Duane R., Redon, Christophe, Ried, Thomas, Bonner, William M., Honjo, Tasuku, Nussenzweig, Michael C., and Nussenzweig, Andre
- Subjects
DNA repair ,ENZYMES - Abstract
Investigates the role of activation-induced cytidine deaminase in facilitating DNA double-strand break repair. Co-localization of Nijmegen breakage syndrome protein and phosphorylated H2A histone family member X foci with genomic loci; Kinetics of immunoglobulin heavy-chain constant region gene.
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- 2001
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235. A hallmark of active class switch recombination: Transcripts directed by I promoters on looped- ....
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Kinoshita, Kazuo, Harigai, Motoko, Fagarasan, Sidonia, Muramatsu, Masamichi, and Honjo, Tasuku
- Subjects
GENETIC transcription ,CIRCULAR DNA - Abstract
Examines transcripts directed by I promoters on looped-out circular DNA. Dependence of circle transcripts on activation-induced cytidine deaminase; Comparision between circle transcripts and circular DNA; Implications of circle transcrips for class switch recombination.
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- 2001
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236. Prolonged Gut Dysbiosis and Fecal Excretion of Hepatitis A Virus in Patients Infected with Human Immunodeficiency Virus.
- Author
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Ishizaka, Aya, Koga, Michiko, Mizutani, Taketoshi, Lim, Lay Ahyoung, Adachi, Eisuke, Ikeuchi, Kazuhiko, Ueda, Ryuta, Aoyagi, Haruyo, Tanaka, Satoshi, Kiyono, Hiroshi, Matano, Tetsuro, Aizaki, Hideki, Yoshio, Sachiyo, Mita, Eiji, Muramatsu, Masamichi, Kanto, Tatsuya, Tsutsumi, Takeya, and Yotsuyanagi, Hiroshi
- Subjects
HIV ,HEPATITIS A virus ,DYSBIOSIS ,VIRAL hepatitis ,HEPATITIS viruses ,GUT microbiome ,INTESTINAL mucosa ,FECES - Abstract
Hepatitis A virus (HAV) causes transient acute infection, and little is known of viral shedding via the duodenum and into the intestinal environment, including the gut microbiome, from the period of infection until after the recovery of symptoms. Therefore, in this study, we aimed to comprehensively observe the amount of virus excreted into the intestinal tract, the changes in the intestinal microbiome, and the level of inflammation during the healing process. We used blood and stool specimens from patients with human immunodeficiency virus who were infected with HAV during the HAV outbreak in Japan in 2018. Moreover, we observed changes in fecal HAV RNA and quantified the plasma cytokine level and gut microbiome by 16S rRNA analysis from clinical onset to at least 6 months after healing. HAV was detected from clinical onset up to a period of more than 150 days. Immediately after infection, many pro-inflammatory cytokines were elicited, and some cytokines showed different behaviors. The intestinal microbiome changed significantly after infection (dysbiosis), and the dysbiosis continued for a long time after healing. These observations suggest that the immunocompromised state is associated with prolonged viral shedding into the intestinal tract and delayed recovery of the intestinal environment. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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237. Immunogenicity and Antigenicity of Rabbit Hepatitis E Virus-Like Particles Produced by Recombinant Baculoviruses.
- Author
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Bai, Huimin, Kataoka, Michiyo, Ami, Yasushi, Suzaki, Yuriko, Takeda, Naokazu, Muramatsu, Masamichi, and Li, Tian-Cheng
- Subjects
HEPATITIS E ,VIRUS-like particles ,BACULOVIRUSES ,HEPATITIS E virus ,RABBITS - Abstract
Rabbit hepatitis E virus (HEV) is a novel HEV belonging to genotype 3 (HEV-3) in the Orthohepevirus A species of the genus Hepevirus, family Hepeviridae. Rabbit HEV was originally isolated from rabbits and found to cause zoonotic infection. Although rabbit HEV can be successfully grown in culture with several cell lines, including the human carcinoma cell line PLC/PRF/5, it is difficult to obtain the large amounts of viral antigen required for diagnosis and vaccine development. In this study, we expressed N-terminal 13 and 111 aa-truncated rabbit HEV ORF2 proteins using recombinant baculoviruses and obtained two types of virus-like particles (VLPs), RnVLPs and RsVLPs with ~35 and 24 nm diameter, respectively. Anti-rabbit HEV IgG antibodies were induced in high titer by immunizing rabbits with RnVLPs or RsVLPs. The antibody secretion in the serum persisted more than three years. RsVLPs showed stronger antigenic cross-reactivity against HEV-1, HEV-3 and HEV-4 than rat HEV. Moreover, anti-RsVLPs antibodies neutralized not only the cognate virus but also HEV-1, HEV-3 and HEV-4 ex vivo, indicating that rabbit HEV had the same serotype as human HEVs. In contrast, the antibody did not block rat HEV infection, demonstrating that rat HEV belonged to a different serotype. Animal experiments indicated that immunization with either RnVLPs or RsVLPs completely protected the rabbits from challenge by rabbit HEV, suggesting that the VLPs are candidates for rabbit HEV vaccine development. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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238. IgG and IgE Collaboratively Accelerate Expulsion of Strongyloides venezuelensisin a Primary Infection
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Matsumoto, Makoto, Sasaki, Yuki, Yasuda, Koubun, Takai, Toshiyuki, Muramatsu, Masamichi, Yoshimoto, Tomohiro, and Nakanishi, Kenji
- Abstract
ABSTRACTThe host deploys a subset of immune responses to expel helminths, which differs depending on the nature of the helminth. Strongyloides venezuelensis, a counterpart of the human pathogen S. stercoralis, naturally infects rodents and has been used as an experimental model. Here we show that induction of immunoglobulin G (IgG) and IgE is a prerequisite for rapid expulsion of S. venezuelensisduring a primary infection. Activation-induced cytidine deaminase-deficient (AID-/-) mice, which lack the ability to switch IgM to other isotypes, normally developed T-helper 2 (Th2) cells and intestinal mastocytosis after infection with S. venezuelensis. Although AID-/-mice expelled Nippostrongylus brasiliensisnormally, they required a much longer period to expel S. venezuelensisthan wild-type (WT) mice. Adoptive transfers of immune sera from S. venezuelensis-infected but not N. brasiliensis-infected mice restored the ability of AID-/-mice to promptly expel S. venezuelensis. Immune serum-derived IgG and IgE induced worm expulsion via Fc ? receptor III (Fc?RIII) and Fc e receptor I (FceRI), respectively, and a mixture of IgG and IgE showed collaborative effects. Whereas Fc?RIII-/-mice or FceRIa-/-mice normally could expel S. venezuelensis, Fc?RIII-/-mice, when their IgE was neutralized by anti-IgE, or FceRIa-/-mice, when their IgG binding to Fc?RIII was blocked by anti-Fc?RIII, showed a markedly reduced ability to expel S. venezuelensis. These data reveal that IgG and IgE play redundant roles but act in concert to accelerate S. venezuelensisexpulsion. Mast cell-deficient mice, even those equipped with immune serum-derived IgG or IgE, failed to expel S. venezuelensispromptly, suggesting that mast cells are cellular targets of IgG and IgE.
- Published
- 2013
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239. Aid How does it aid antibody diversity?
- Author
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Honjo, Tasuku, Muramatsu, Masamichi, and Fagarasan, Sidonia
- Abstract
Activation-induced cytidine deaminase (AID) is an essential enzyme to regulate class switch recombination (CSR), somatic hypermutation (SHM), and gene conversion (GC). AID is known to be required for DNA cleavage of S regions in CSR. However, its molecular mechanism is a focus of extensive debate. RNA editing hypothesis postulates that AID edits yet unknown mRNA to generate specific endonucleases for CSR and SHM. By contrast, DNA deamination hypothesis assumes that AID deaminates cytosine in DNA, followed by DNA cleavage by base excision repair enzymes. We discuss available evidence for the two proposed models. Recent findings, namely requirement of protein synthesis for DNA breakage and dispensability of U removal activity of uracil DNA glycosylase, force us to reconsider DNA deamination hypothesis.
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240. MCPIP1 reduces HBV-RNA by targeting its epsilon structure.
- Author
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Li, Yingfang, Que, Lusheng, Fukano, Kento, Koura, Miki, Kitamura, Kouichi, Zheng, Xin, Kato, Takanobu, Aly, Hussein Hassan, Watashi, Koichi, Tsukuda, Senko, Aizaki, Hideki, Watanabe, Noriyuki, Sato, Yuko, Suzuki, Tadaki, Suzuki, Hiroshi I., Hosomichi, Kazuyoshi, Kurachi, Makoto, Wakae, Kousho, and Muramatsu, Masamichi
- Subjects
HEPATITIS B virus ,VIRAL hepatitis ,CIRRHOSIS of the liver ,LIVER cancer ,CYTOKINES ,LIVER cells - Abstract
Hepatitis B virus (HBV) is the major causative factor of chronic viral hepatitis, liver cirrhosis, and hepatocellular carcinoma. We previously demonstrated that a proinflammatory cytokine IL-1β reduced the level of HBV RNA. However, the mechanism underlying IL-1β-mediated viral RNA reduction remains incompletely understood. In this study, we report that immune regulator Monocyte chemotactic protein-1-induced protein 1 (MCPIP1) can reduce HBV RNA in hepatocytes. MCPIP1 expression level was higher in the liver tissue of HBV-infected patients and mice. Overexpression of MCPIP1 decreased HBV RNA, whereas ablating MCPIP1 in vitro enhanced HBV production. The domains responsible for RNase activity or oligomerization, were required for MCPIP1-mediated viral RNA reduction. The epsilon structure of HBV RNA was important for its antiviral activity and cleaved by MCPIP1 in the cell-free system. Lastly, knocking out MCPIP1 attenuated the anti-HBV effect of IL-1β, suggesting that MCPIP1 is required for IL-1β-mediated HBV RNA reduction. Overall, these results suggest that MCPIP1 may be involved in the antiviral effect downstream of IL-1β. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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241. Prediction of hand, foot, and mouth disease epidemics in Japan using a long short-term memory approach.
- Author
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Yoshida, Kazuhiro, Fujimoto, Tsuguto, Muramatsu, Masamichi, and Shimizu, Hiroyuki
- Subjects
- *
COXSACKIEVIRUS diseases , *FOOT & mouth disease , *MENINGITIS , *RECURRENT neural networks , *COMMUNICABLE diseases , *ORAL mucosa , *REPORTING of diseases , *DEEP learning - Abstract
Hand, foot, and mouth disease (HFMD) is a common febrile illness caused by enteroviruses in the Picornaviridae family. The major symptoms of HFMD are fever and a vesicular rash on the hand, foot, or oral mucosa. Acute meningitis and encephalitis are observed in rare cases. HFMD epidemics occur annually in Japan, usually in the summer season. Relatively large-scale outbreaks have occurred every two years since 2011. In this study, the epidemic patterns of HFMD in Japan are predicted four weeks in advance using a deep learning method. The time-series data were analyzed by a long short-term memory (LSTM) approach called a Recurrent Neural Network. The LSTM model was trained on the numbers of weekly HFMD cases in each prefecture. These data are reported in the Infectious Diseases Weekly Report, which compiles the national surveillance data from web sites at the National Institute of Infectious Diseases, Japan, under the Infectious Diseases Control Law. Consequently, our trained LSTM model distinguishes between relatively large-scale and small-scale epidemics. The trained model predicted the HFMD epidemics in 2018 and 2019, indicating that the LSTM approach can estimate the future epidemic patterns of HFMD in Japan. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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242. Hepatitis E virus infection in 6-month-old pigs in Taiwan.
- Author
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Liao, Ming-Hui, Wu, Fang-Tzy, Bai, Huimin, Doan, Yen Hai, Yang, Jyh-Yuan, Takeda, Naokazu, Muramatsu, Masamichi, and Li, Tian-Cheng
- Subjects
HEPATITIS E virus ,GENOTYPES ,SWINE diseases ,REVERSE transcriptase polymerase chain reaction - Abstract
Hepatitis E virus (HEV) is the causative agent of acute hepatitis E. Genotype 3 (G3) and 4 (G4) HEV have recently been identified in and isolated from swine as the main HEV genotypes worldwide. However, there is limited information on HEV infection status among pigs in Taiwan, especially pigs in the stage before transportation to the slaughterhouse. To determine the frequency of HEV infection among pigs in Taiwan, we detected and quantified HEV RNA contained in 295 fecal specimens collected from 6-month-old pigs bred in 30 pig farms located in 8 counties. We found that 25.1% (74/295) of the fecal specimens were positive for HEV RNA by a quantitative real-time reverse transcription-polymerase chain reaction, and the copy number ranged from 2.3 × 10
3 to 2.08 × 107 copies/g. Amplification of a 338 bp sequence in ORF2 was achieved in 16 of 74 HEV RNA-positive samples, and their nucleotide sequences were determined. Two HEV sequences appeared to belong to subtype 3a of G3 and the remaining 14 HEV sequences belonged to subtype 4b of G4 (G4b). The entire genome sequence of two G4b HEVs was obtained by next-generation sequence analyses, and the phylogenetic analyses indicated that unique G4b HEVs were circulating in pig farms in Taiwan. In the present study, we found that both G3 and G4 HEVs were circulating in Taiwanese pig farms and G4b was the predominant subtype. In addition, the relatively high detection frequency of HEV RNA in the 6-month-old pigs indicated that Taiwanese pigs just before transportation to the slaughterhouse are at risk of carrying HEVs, and thus thorough cooking or heating of pork meat or organs is needed before consumption in Taiwan and possibly in other countries as well. [ABSTRACT FROM AUTHOR]- Published
- 2020
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243. Characterization of the self-assembly of New Jersey polyomavirus VP1 into virus-like particles and the virus seroprevalence in Japan.
- Author
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Zhou, Xianfeng, Bai, Huimin, Kataoka, Michiyo, Ito, Masahiko, Muramatsu, Masamichi, Suzuki, Tetsuro, and Li, Tian-Cheng
- Subjects
MOLECULAR self-assembly ,POLYOMAVIRUSES ,SEROPREVALENCE ,ENDOTHELIAL cells ,RECOMBINANT baculoviruses - Abstract
New Jersey polyomavirus (NJPyV) was discovered in 2014 in a pancreatic transplant recipient's vascular endothelial cells. Here, in the recombinant baculovirus system, VP1 protein of NJPyV expressed in insect cells was processed. The protein self-assembled into virus-like particles (NJPyV-LPs) in a cell-type-dependent manner, and the particles were then released into the culture media. Spherical ~50-nm-dia. NJPyV-LPs of uniform size with morphology resembling that of the native particles of polyomaviruses were purified from the fraction at 1.33 g/cm
3 in supernatants of VP1-expressing Sf9 cells. We investigated the antigenic properties of purified NJPyV-LPs and performed a VLP-based enzyme immunoassay to determine the age-specific prevalence of NJPyV infection in a general Japanese population aged 1–70 years. The overall seropositivity rate of anti-NJPyV antibodies was only 1.8%. This might be explained by the low circulation of NJPyV in Japan. This is the first report of a large-scale serological survey of NJPyV in Asia (n = 1,050). [ABSTRACT FROM AUTHOR]- Published
- 2019
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244. Loxapine inhibits replication of hepatitis A virus in vitro and in vivo by targeting viral protein 2C.
- Author
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Matsuda, Mami, Hirai-Yuki, Asuka, Kotani, Osamu, Kataoka, Michiyo, Zheng, Xin, Yamane, Daisuke, Yokoyama, Masaru, Ishii, Koji, Muramatsu, Masamichi, and Suzuki, Ryosuke
- Subjects
- *
ANTIVIRAL agents , *HEPATITIS viruses , *VIRAL proteins , *VIRAL hepatitis , *DOPAMINE receptors , *HEPATITIS A virus , *VIRAL replication , *INTERFERON receptors - Abstract
No antiviral drugs currently are available for treatment of infection by hepatitis A virus (HAV), a causative agent of acute hepatitis, a potentially life-threatening disease. Chemical screening of a small-compound library using nanoluciferase-expressing HAV identified loxapine succinate, a selective dopamine receptor D2 antagonist, as a potent inhibitor of HAV propagation in vitro. Loxapine succinate did not inhibit viral entry nor internal ribosome entry site (IRES)-dependent translation, but exhibited strong inhibition of viral RNA replication. Blind passage of HAV in the presence of loxapine succinate resulted in the accumulation of viruses containing mutations in the 2C-encoding region, which contributed to resistance to loxapine succinate. Analysis of molecular dynamics simulations of the interaction between 2C and loxapine suggested that loxapine binds to the N-terminal region of 2C, and that resistant mutations impede these interactions. We further demonstrated that administration of loxapine succinate to HAV-infected Ifnar1-/- mice (which lack the type I interferon receptor) results in decreases in the levels of fecal HAV RNA and of intrahepatic HAV RNA at an early stage of infection. These findings suggest that HAV protein 2C is a potential target for antivirals, and provide novel insights into the development of drugs for the treatment of hepatitis A. Author summary: Hepatitis A outbreaks have occurred world-wide, not only in developing countries but also in developed countries, resulting in 15,000–30,000 mortalities annually. However, no antiviral drugs currently are available for the treatment of hepatitis A virus (HAV) infection. Using a reporter-based recombinant HAV, a chemical library of pharmacologically active compounds was screened to identify drugs that specifically inhibit HAV propagation. We show here that loxapine succinate, a tricyclic antipsychotic medication that has been approved by the US FDA for the treatment of schizophrenia, is a potent inhibitor of HAV replication. The effect of loxapine on HAV appears to be independent of the dopamine D2 receptor (the target of the antipsychotic activity), instead corresponding to affinity for the HAV 2C protein, as demonstrated by molecular analysis of loxapine-resistant virus as well as in silico modeling. We further showed that administration of loxapine succinate to HAV-infected Ifnar1-/- mice (which lack the type 1 interferon receptor) resulted in decreases in the levels of fecal HAV RNA and of intrahepatic HAV RNA at an early stage. Together, these results suggest a new target for the development of therapeutics for the treatment of HAV-infected patients. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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- View/download PDF
245. Chimeric provirus of bovine leukemia virus/SMAD family member 3 in cattle with enzootic bovine leukosis.
- Author
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Nao, Naganori, Okagawa, Tomohiro, Nojiri, Naomi, Konnai, Satoru, Shimakura, Honami, Tominaga, Misono, Yoshida-Furihata, Hazuka, Nishiyama, Eri, Matsudaira, Takahiro, Maekawa, Naoya, Murata, Shiro, Muramatsu, Masamichi, Ohashi, Kazuhiko, and Saito, Masumichi
- Abstract
Bovine leukemia virus (BLV) is a member of the family Retroviridae that causes enzootic bovine leukemia (EBL). However, the association between BLV infection and EBL development remains unclear. In this study, we identified a BLV/SMAD3 chimeric provirus within CC2D2A intron 30 in monoclonal expanded malignant cells from a cow with EBL. The chimeric provirus harbored a spliced SMAD3 sequence composed of exons 3–9, encoding the short isoform protein, and the BLV-SMAD3 chimeric transcript was detectable in cattle with EBL. This is the first report of a BLV chimeric provirus that might be involved in EBL tumorigenesis. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
246. Enterovirus A71 does not meet the uncoating receptor SCARB2 at the cell surface.
- Author
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Nishimura, Yorihiro, Sato, Kei, Koyanagi, Yoshio, Wakita, Takaji, Muramatsu, Masamichi, Shimizu, Hiroyuki, Bergelson, Jeffrey M., and Arita, Minetaro
- Subjects
- *
CELL receptors , *P-selectin glycoprotein ligand-1 , *MEMBRANE proteins , *PROTEIN receptors , *VIRUS diseases , *FOOT & mouth disease - Abstract
Enterovirus A71 (EV-A71) infection involves a variety of receptors. Among them, two transmembrane protein receptors have been investigated in detail and shown to be critical for infection: P-selectin glycoprotein ligand-1 (PSGL-1) in lymphocytes (Jurkat cells), and scavenger receptor class B member 2 (SCARB2) in rhabdomyosarcoma (RD) cells. PSGL-1 and SCARB2 have been reported to be expressed on the surface of Jurkat and RD cells, respectively. In the work reported here, we investigated the roles of PSGL-1 and SCARB2 in the process of EV-A71 entry. We first examined the expression of SCARB2 in Jurkat cells, and detected it within the cytoplasm, but not on the cell surface. Further, using PSGL-1 and SCARB2 knockout cells, we found that although both PSGL-1 and SCARB2 are essential for virus infection of Jurkat cells, virus attachment to these cells requires only PSGL-1. These results led us to evaluate the cell surface expression and the roles of SCARB2 in other EV-A71–susceptible cell lines. Surprisingly, in contrast to the results of previous studies, we found that SCARB2 is absent from the surface of RD cells and other susceptible cell lines we examined, and that although SCARB2 is essential for infection of these cells, it is dispensable for virus attachment. These results indicate that a receptor other than SCARB2 is responsible for virus attachment to the cell and probably for internalization of virions, not only in Jurkat cells but also in RD cells and other EV-A71–susceptible cells. SCARB2 is highly concentrated in lysosomes and late endosomes, where it is likely to trigger acid-dependent uncoating of virions, the critical final step of the entry process. Our results suggest that the essential interactions between EV-A71 and SCARB2 occur, not at the cell surface, but within the cell. Author summary: Enterovirus A71 (EV-A71) is a major pathogen of hand-foot-and-mouth disease. Although a variety of receptor molecules have been proposed to mediate EV-A71 infection, one of them, SCARB2, is widely believed to serve as the primary attachment and entry receptor on many cell types. SCARB2—initially identified as a lysosomal integral membrane protein, LIMP II—is primarily expressed within the cytoplasm rather than on the cell surface. In this study, we examined how this largely cytoplasmic protein functions during EV-A71 attachment and infection. In contrast to earlier reports, we found that SCARB2 was not expressed at detectable levels on the surface of EV-A71–susceptible cell lines, and that its elimination with CRISPR/Cas9 had no effect on attachment of virions to the cell surface. Nonetheless, SCARB2 was readily detected on late endosomes and lysosomes, and is essential for productive infection. The results suggest that non-SCARB2 receptors are responsible for virus attachment and internalization, whereas SCARB2 within late endosomes and lysosomes is an intracellular receptor essential for a subsequent event in infection. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
247. Generation of JC Polyoma Pseudovirus for High-Throughput Measurement of Neutralizing Antibodies.
- Author
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Matsuda, Mami, Li, Tian-Cheng, Nakanishi, Akira, Nakamichi, Kazuo, Saito, Makoto, Suzuki, Tadaki, Matsuura, Tomokazu, Muramatsu, Masamichi, Suzuki, Tetsuro, Miura, Yoshiharu, and Suzuki, Ryosuke
- Subjects
- *
PROGRESSIVE multifocal leukoencephalopathy , *CENTRAL nervous system diseases , *BK virus , *POLYOMAVIRUSES , *ENZYME-linked immunosorbent assay - Abstract
Progressive multifocal leukoencephalopathy (PML) is a demyelinating disease of the central nervous system (CNS) caused by reactivation of dormant JC polyomavirus (JCPyV). PML was mainly observed in immunocompromised individuals, such as HIV-positive patients, autoimmune disease patients, and cancer patients. Given that the presence of anti-JCPyV antibodies in serum is a risk indicator for PML development, it is essential to monitor anti-JCPyV antibody levels. In the present study, we established reporter-based single-infection neutralization assays for JCPyV and the genetically similar BK polyoma virus (BKPyV). We then confirmed the lack of cross-reactivity between the two viruses using test sera obtained from mice immunized with plasmids encoding the JCPyV or BKPyV capsid. Next, we compared neutralization antibody titers in sera from healthy donors, patients with multiple sclerosis (MS), and HIV-positive patients using an in-house enzyme-linked immunosorbent assay (ELISA) with JCPyV-like particles (virus-like particles; VLPs). A positive correlation was demonstrated between the neutralization titer (75% infectious concentration; IC75) against JCPyV and the antibody titer obtained by VLP-based JCPyV ELISA. This assay system may be applied to detect antibodies against other PyVs by generation of pseudoviruses using the respective capsid expression plasmids, and is expected to contribute to the surveillance of PyV as well as basic research on these viruses. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
248. A novel splenic B1 regulatory cell subset suppresses allergic disease through phosphatidylinositol 3-kinase–Akt pathway activation.
- Author
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Matsushita, Takashi, Le Huu, Doanh, Kobayashi, Tadahiro, Hamaguchi, Yasuhito, Hasegawa, Minoru, Naka, Kazuhito, Hirao, Atsushi, Muramatsu, Masamichi, Takehara, Kazuhiko, and Fujimoto, Manabu
- Abstract
Background IL-10–producing regulatory B (B10) cells potently suppress allergic diseases, such as contact hypersensitivity (CHS). Splenic B10 cells share overlapping phenotypic markers with CD5 + B1 B cells, CD1d hi CD21 + CD23 − marginal zone (MZ) B cells, and CD1d hi CD21 + CD23 + T2-MZ precursor B cells but do not exclusively belong to either subset. Objective In this study we investigated the signaling mechanisms and a novel phenotypic parameter of B10 cells. Method We performed microarray analysis comparing IL-10 + and IL-10 − B cells. B cell–specific phosphatase and tensin homolog (PTEN)–deficient mice, which exhibit aberrant activation of the phosphatidylinositol 3-kinase (PI3K)–Akt pathway in B cells, were examined. Results Microarray analysis revealed that the PI3K-Akt pathway is important for IL-10 production in B cells. PI3K-Akt pathway inhibitors reduced B10 cell numbers in vitro . B10 cell numbers were significantly increased in B cell–specific PTEN-deficient mice. The CHS response was significantly diminished in PTEN-deficient mice. Unexpectedly, splenic B10 cells in these mice were found within the B1 B-cell subset but not within the MZ B-cell subset. In wild-type mice not only MZ B10 cells but also B1-B10 cells were identified in the spleen. In addition, these 2 B10 cell subsets were predominantly found within the CD9 + CD80 + B-cell fraction. Conclusion A novel splenic B1 regulatory cell subset (B1-B10 cells) was identified. Our findings show that the PI3K-Akt pathway in B cells is critical for B10 cell development and CHS response and that CD9/CD80 coexpression is a novel phenotypic parameter for both MZ-B10 and B1-B10 cells. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
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249. Selective inhibition of hepatitis B virus internalization by oxysterol derivatives.
- Author
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Oshima, Mizuki, Stappenbeck, Frank, Ohashi, Hirofumi, Iwamoto, Masashi, Fukano, Kento, Kusunoki, Atsuto, Zheng, Xin, Wang, Feng, Morishita, Ryo, Aizaki, Hideki, Suzuki, Ryosuke, Muramatsu, Masamichi, Kuramochi, Kouji, Sureau, Camille, Parhami, Farhad, and Watashi, Koichi
- Subjects
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HEPATITIS D , *HEPATITIS B virus , *HEPATITIS A , *ORAL drug administration , *HEPATITIS B - Abstract
Given that the current approved anti-hepatitis B virus (HBV) drugs suppress virus replication and improve hepatitis but cannot eliminate HBV from infected patients, new anti-HBV agents with different mode of action are urgently needed. In this study, we identified a semi-synthetic oxysterol, Oxy185, that can prevent HBV infection in a HepG2-based cell line and primary human hepatocytes. Mechanistically, Oxy185 inhibited the internalization of HBV into cells without affecting virus attachment or replication. We also found that Oxy185 interacted with an HBV entry receptor, sodium taurocholate cotransporting polypeptide (NTCP), and inhibited the oligomerization of NTCP to reduce the efficiency of HBV internalization. Consistent with this mechanism, Oxy185 also inhibited the hepatitis D virus infection, which relies on NTCP-dependent internalization, but not hepatitis A virus infection, and displayed pan-genotypic anti-HBV activity. Following oral administration in mice, Oxy185 showed sustained accumulation in the livers of the mice, along with a favorable liver-to-plasma ratio. Thus, Oxy185 is expected to serve as a useful tool compound in proof-of-principle studies for HBV entry inhibitors with this novel mode of action. • Oxy185 inhibited HBV infection through reducing viral internalization. • Oxy185 directly interacted with an HBV receptor, NTCP. • Oxy185 inhibited the oligomerization of NTCP. • Oxy185 showed broad antiviral activity to multiple HBV isolates. • Oxy185 showed preferable pharmacokinetics, accumulated in liver of oral treated mice. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
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250. Inhibitory effect of Ephedra herba on human norovirus infection in human intestinal organoids.
- Author
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Hayashi, Tsuyoshi, Murakami, Kosuke, Ando, Hirokazu, Ueno, Sayuri, Kobayashi, Sakura, Muramatsu, Masamichi, Tanikawa, Takashi, and Kitamura, Masashi
- Subjects
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NOROVIRUS diseases , *EPHEDRA , *INTESTINAL infections , *FOODBORNE diseases , *ORGANOIDS - Abstract
Human norovirus (HuNoV) is a major cause of acute gastroenteritis and foodborne diseases worldwide with public health concern, yet no antiviral therapies have been developed. In this study, we aimed to screen crude drugs, which are components of Japanese traditional medicine, "Kampo" to see their effects on HuNoV infection using a reproducible HuNoV cultivation system, stem-cell derived human intestinal organoids/enteroids (HIOs). Among the 22 crude drugs tested, Ephedra herba significantly inhibited HuNoV infection in HIOs. A time-of-drug addition experiment suggested that this crude drug more preferentially targets post-entry step than entry step for the inhibition. To our knowledge, this is the first anti-HuNoV inhibitor screen targeting crude drugs, and Ephedra herba was identified as a novel inhibitor candidate that merits further study. • 22 crude drugs were tested for their ability to inhibit HuNoV infection in HIOs. • Ephedra herba was identified as a novel anti-HuNoV inhibitor candidate. • Ephedra herba preferentially targets post-entry step for the inhibition. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
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