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This article presents studies on the evaluation of the impact of surface modification of cotton, viscose, and polyester fabrics using three techniques (flocking, layer by layer, and screen printing) with materials with electrically conductive properties on their structural, biophysical, and conductive properties. Each tested fabric is characterized by specific biophysical properties. which can be disturbed by various modification methods, therefore, the following tests were carried out in the article: optical microscopy, micro-computed tomography, guarded perspiration heating plate, air permeability, sorption and electrical conductivity tester. The use of screen printing increased the thermal resistance of the cotton woven fabric by 119%, the polyester woven fabric by 156%, and the viscose fabric by 261%. The smallest changes in thermal resistance compared to unmodified textiles were observed in layer by layer modified fabrics and are as follows: −15% (cotton woven fabric), +77% (PES woven fabric), and +80% (viscose woven fabric). [ABSTRACT FROM AUTHOR]

The application of biobased and biodegradable polymers, such as polylactide (PLA), in fused deposition modeling (FDM) 3D-printing technology creates a new prospect for rapid prototyping and other applications in the context of ecology. The popularity of the FDM method and its significance in material engineering not only creates new prospects for the development of technical sciences on an industrial scale, but also introduces new technologies into households. In this study, the kinetics of the hydrolytic degradation of samples obtained by the FDM method from commercially available PLA filaments under a thermally accelerated regime were analyzed. The investigation was conducted at the microstructural, supramolecular, and molecular levels by using methods such as micro-computed tomography (micro-CT), wide-angle X-ray diffraction (WAXD), viscosimetry, and mass erosion measurements. The obtained results clearly present the rapid structural changes in 3D-printed materials during degradation due to their amorphous initial structure. The complementary studies carried out at different scale levels allowed us to demonstrate the relationship between the observed structural changes in the samples and the hydrolytic decomposition of the polymer chains, which made it possible to scientifically understand the process and expand the knowledge on biodegradation. [ABSTRACT FROM AUTHOR]

The aim of this study was to evaluate the remineralizing properties of ion-releasing restorative materials on pH cycling-induced carious dentin. Fifty sound molars were freshly extracted. The occlusal surfaces were abraded using water-cooled sandpaper (800 grit). The residual crowns were embedded in self-cured acrylic resin with the flat dentin surface exposed. A mesio-distal trench was created using a calibrated 0.5 mm deep occlusal reduction burr, and artificial dentin caries were generated by pH cycling. Then, teeth were randomly assigned to five groups according to the ion-releasing material used. For each sample, micro-CT acquisitions were performed at various intervals. Remineralization was assessed by mean gray value (MGV) measurements after registration and segmentation of the region of interest with 3D Slicer software. One-way repeated-measures ANOVA followed by Tukey's post hoc test was used to investigate the difference in MGVs among the various groups. Only Cention Forte showed significantly increased MGVs after 4 weeks compared to demineralized dentin. MGVs were higher, but not significantly, after placement of the restorative materials, including in the resin composite control group. These results can be explained by the radiopacity of the materials. Cention Forte, the material with the highest radiopacity, showed a significant increase in the MGVs of artificially carious dentin after 4 weeks. However, the study of dentin remineralization by micro-CT could be impacted by the radiopacity of the restorative materials used. The relevance of this examination for the study of dentinal remineralization should be investigated. [ABSTRACT FROM AUTHOR]

Aims: To compare and evaluate marginal and internal adaptation and to assess the degree of conversion of alkasite restoration in relation with other restorative materials. Methods: on the buccal surface of 25 maxillary premolars a class V cavities were prepared. The teeth were randomly divided into five groups (n=5) that restored as: Group 1: alkasite without adhesive, Group 2: alkasite with adhesive, Group 3: Nanohybrid composite, Group 4: Glass ionomer cement, and Group 5: Resin modified glass ionomer cement. The teeth underwent 5000 cycles of thermocycling between 5° and 55°. Then, silver nitrate solution was infiltrated and Micro-Computed tomography analysis was performed using (LOTUS inVivo). For evaluating degree of conversion, 15 specimens in the form of a disc were prepared from Alkasite, nanohybrid composite and resin modified glass ionomer (n=5). All samples were analyzed using (FTIR) in an ATR Mode in three intervals (after 20 second. 24 hours and 7 days). The data had been analyzed using the Kruskal-Wallis, Dune and Wilcoxon tests at a 0.05 significance level. Result: A significant difference in marginal and internal adaptation were observed among restorations (p < 0.05). Superior marginal and internal adaptation was obtained in this study for alkasite whether with or without bonded over nanohybrid composite. However, RMGIC and GIC show more adaptation values among other tested groups. Also degree of monomer conversion percentage varied among restorations at different intervals. Conclusion: alkasite restorations wither with or without bonding have higher marginal and internal adaptation in comparison with nanohybrid composite but lower than that of GIC and RMGIC. Also alkasite restoration showed higher degree of conversion when compared with nanohybride composite and RMGIC after 20 second and 24 hours of polymerization. [ABSTRACT FROM AUTHOR]

Objectives: The aim of this study was to assess cone beam computed tomography (CBCT) as a root canal anatomy diagnostic tool by comparison with micro-CT gold-standard. Materials and methods: 216 two-rooted mandibular molars were first scanned in a CBCT device (200 μm voxel size) and posteriorly in a micro-CT scanner (19.61 μm). The volumes were sequentially screened to classify main root canal anatomy according to Vertucci classification, and for the presence of lateral canals and apical deltas, in both mesial and distal roots. Results: Both methods revealed a higher prevalence of Vertucci Type II and IV in the mesial root, and Vertucci Type I in the distal root. The percentage of agreement for main root canal anatomy classification between CBCT and micro-CT scores was high (85.2%). Conclusion: Sensibility to detect both lateral canals and apical deltas with CBCT was low. These results attest to the fact that minor anatomical changes might be difficult to identify with CBCT imaging, hampering its diagnostic value. [ABSTRACT FROM AUTHOR]

The amalgamation of veterinary anatomy, technology and innovation has led to development of latest technological advancement in the field of veterinary medicine, i.e., three-dimensional (3D) imaging and reconstruction. 3D visualization technique followed by 3D reconstruction has been proven to enhance non-destructive 3D visualization grossly or microscopically, e.g., skeletal muscle, smooth muscle, ligaments, cartilage, connective tissue, blood vessels, nerves, lymph nodes, and glands. The core aim of this manuscript is to document non-invasive 3D visualization methods being adopted currently in veterinary anatomy to reveal underlying morphology and to reconstruct them by 3D softwares followed by printing, its applications, current challenges, trends and future opportunities. 3D visualization methods such as MRI, CT scans and micro-CT scans are utilised in revealing volumetric data and underlying morphology at microscopic levels as well. This will pave a way to transform and re-invent the future of teaching in veterinary medicine, in clinical cases as well as in exploring wildlife anatomy. This review provides novel insights into 3D visualization and printing as it is the future of veterinary anatomy, thus making it spread to become the plethora of opportunities for whole veterinary science. [ABSTRACT FROM AUTHOR]

Background: Trabeculae in vertebral bodies are unequally distributed within the cervical spine (CS), the thoracic spine (TS), and lumbar spine (LS). Such structures are also unequally distributed within the individual vertebrae. Exact knowledge of the microstructure of these entities could impact our understanding and treatment of fractures caused by osteoporosis and possibly improve surgical approaches. Appropriate investigations could help clarify the pathomechanisms of different forms of osteoporotic vertebral fractures, as well as different changes in morphological findings like the trabecular bone score (TBS). In the present study, we applied punctures to the craniocaudal and ventrocaudal directions and obtained cylinders of cancellous bone from the central portions and marginal regions of cervical vertebrae 5 and 6, thoracic vertebrae 8 and 12, and lumbar vertebrae 1 and 3. We systematically analyzed these samples to determine the bone volume fraction, trabecular thickness, separation, connectivity density, degree of anisotropy, and structure model index. Methods: Using an 8-gauge Jamshidi needle, we obtained samples from three quadrants (Q I: right margin; Q II: central; Q III: left margin) in the frontal and transverse plane and prepared these samples with a moist cloth in a 1.5 mL Eppendorf reaction vessel. The investigations were performed on a micro-CT device (SKYSCAN 1172, RJL Micro & Analytic Company, Karlsdorf-Neuthard, Germany). All collected data were analyzed using the statistical software package SPSS (version 24.0, IBM Corp., Armonk, NY, USA). Student's t test, the Wilcoxon–Mann–Whitney test, the Chi-squared test, and univariate analysis were used for between-group comparisons. The selection of the test depended on the number of investigated groups and the result of the Shapiro–Wilk test of normal distribution. In the case of statistically significant results, a post hoc LSD test was performed. Results: In total, we obtained 360 bone samples from 20 body donors. The craniocaudal puncture yielded data of similar magnitudes for all investigated parameters in all three quadrants, with the highest values observed in the CS. Comparisons of the ventrodorsal and craniocaudal microstructure revealed a significantly lower trabecular density and a significantly higher degree of anisotropy in the craniocaudal direction. Conclusions: The results presented different distributions and behaviors of trabecular density, with lower density in the mid-vertebral region over the entire breadth of the vertebrae. Reduced trabecular density caused a higher degree of anisotropy and was, therefore, associated with a lower capacity to sustain biomechanical loads. Fractures in fish vertebrae were easily explained by this phenomenon. The different changes in these structures could be responsible, in part, for the changes in the TBS determined using dual-energy X-ray absorptiometry. These results confirm the clinical relevance of the TBS. [ABSTRACT FROM AUTHOR]

Endoparasitic annelids living inside another annelid host are known, particularly with regard to Oenonidae, but in general are poorly studied. The dorvilleid Veneriserva pygoclava is known from southern California, and its genus name (Latin = Venus's servant) alludes to the close association with the host aphroditid scaleworm Aphrodita longipalpa. Little is known on fundamental questions on the biology of Veneriserva pygoclava. What is its mode of reproduction? How do they feed? How do they penetrate the host? We have studied multiple parasitized hosts and V. pygoclava specimens, using an integrative approach, combining µCT, histology, and electron microscopy. 3D reconstructions from µCT data of a parasitized Aphrodita show the exact position of the parasites in their natural condition within the host's coelomic cavity. Ultrastructural investigations of the parasites revealed interesting adaptations to their lifestyle such as the complete reduction of their gut, despite the presence of a functional jaw apparatus and a modified epidermis enabling nutrient uptake from the host's coelomic fluid. In addition to these, we also investigated spermatogenesis and oogenesis in V. pygoclava. Sperm morphology indicates an external fertilization of eggs within the coelomic cavity of the host. Mature male and female parasites living inside the same mature host and the presence of juvenile V. pygoclava within juveniles of Aphrodita suggest an obligate form of parasitism with a very early penetration of the hosts. In addition to our detailed morphological investigation, we conducted a phylogenetic analysis showing the position of Veneriserva within Dorvilleidae and its position was recovered nested among taxa of the Iphitime. Our phylogenetic analyses also show that the taxation Ophryotrocha puerilis siberti should be given full species rank and referred to as Ophryotrocha siberti. Finally, we publish here the full mitochondrial genome of V. pygoclava and discuss its novel gene order with reference to other annelids. [ABSTRACT FROM AUTHOR]

This study focuses on characterizing a discontinuity within the Seán stalagmite (4.75–7.75 cm) by means of two nondestructive techniques: (1) high-resolution micro-computed tomography (micro-CT) and (2) X-ray fluorescence (XRF) core scanning (XRFCS). Micro-CT was used to study the stalagmite density, and XRFCS was applied to obtain the qualitative elemental composition and colour measurements. The new data obtained from nondestructive techniques have been combined with previously published geochemical data and fabric determinations from the same stalagmite found in Sa Balma des Quartó cave in Mallorca. The two methodologies applied in the present study have improved the characterization of the distinctive horizon. The micro-CT images identified the layer as a minor event due the high air content. The distinctive horizon is characterized by a high Ti-content, indicating the arrival of terrigenous particles. Based on those observations, together with the fact that the micrite layer appears filling the gaps between the older columnar fabric, we argue that the micrite layer may represent a major flooding event inside the cave after the year 1616 ± 23 CE and before the year 1623 ± 28 CE, which can be related to an extreme rainfall event. This hypothesis is further supported by the observed cave flooding during the autumn of 2018. [ABSTRACT FROM AUTHOR]

Porous materials are widely used to treat phosphorus-containing wastewater due to their structural characteristics and loading site functionality. The use of iron tailings as a raw material during the preparation of these materials can effectively reduce production costs. In this study, a porous material was prepared according to a one-step method using solid waste iron tailings. High-temperature phase transition technology was used to construct crystallographic sites on the pore walls of the material to improve the phosphorus removal efficiency. The pore structure, pore wall characteristics, and internal crystal growth in the three-dimensional space of the material were characterized using X-ray microscopy (micro-CT) scanning, and the voxel data for the internal structural were analyzed. The results showed that the material had a clear internal skeletal structure, a uniform distribution of internal pores, and crystal phases in three-dimensional space. The pore ratio in the three-axis direction was greater than 50%, with connected pores accounting for more than 95% of the pores. The wall thickness distribution was uniform, but the pore size distribution and ellipticity ratio varied significantly due to the different pore structures and quantities. A connected structure was formed when the thin walls of adjacent pores ruptured, and there were magnesioferrite and hematite crystal sites with fractal growth in the pore wall framework. The adsorption capacity of the porous material for phosphorus pollutants was investigated using photometry, and the highest removal rate was found to be 68.13%. This analysis suggests that the presence of rough pore walls, a high specific surface area, and crystal sites within the pore walls enhance the adsorption performance of the material. Further research along this line could explore the potential of developing new low-cost materials for pollution treatment. [ABSTRACT FROM AUTHOR]

The purpose of this study was to evaluate the ability of the Reciproc, Reciproc Blue, and WaveOne Gold systems to remove filling material during endodontic retreatment of extracted human mandibular premolars. Thirty-nine teeth were instrumented with the Protaper Universal System to the F3 file and filled with the Tagger hybrid technique using an F3 gutta-percha cone and AH Plus cement. At the end of this period, the teeth were scanned with micro–computed tomography before and after removal of the filling material from the root canals. The teeth were divided into 3 groups (n = 13) based on the apical volume, depending on the systems used to remove the filling material. Group GR: Reciproc 40/.06; Group GRB: Reciproc Blue 40/.06; and Group GWG: WaveOne Gold 35/.06. The results were statistically analyzed using the tests of Kruskal-Wallis, Duncan, and analysis of variance at a significance level of 5%. The results showed that there were no significant differences between the amounts of filling material removed, either for the apical and middle regions alone or in the overall evaluation for the 3 groups (P =.97). The time evaluation statistically showed that the GR and GWG groups required less time to clean the root canals than the GRB group. Reciproc R40 files and WaveOne Gold Medium files required less time for endodontic treatment than Reciproc Blue R40 files. There was no difference in the ability to remove obturation material between the 3 instruments. No instrument was able to completely remove the filling material from the root canals. [ABSTRACT FROM AUTHOR]

Background and Objectives: The aim of this quantitative research was to investigate the effect of gravitational forces on the marginal integrity of different bulk-fill composites by micro-CT imaging. Materials and Methods: Fifty caries-free human third molars extracted for prophylactic purposes were used in this study. Each tooth was prepared with two proximal box cavities, with dimensions of 3 mm × 3 mm × 5 mm. Five distinct groups, each comprising 20 cavities, thus totaling 100 cavities for this study: (1, Group CON): Clearfil Majesty Flow + Clearfil Majesty Esthetic (as the control); (2, Group FBR): Filtek Bulk-fill Flowable Restorative + Clearfil Majesty Esthetic; (3, Group XTB): Voco Extrabase + Clearfil Majesty Esthetic; (4, Group SDR): SDR + Clearfil Majesty Esthetic; and (5, Group SNC): Sonicfill. When restoring the mesial cavities, the occlusal surfaces of the teeth in the mold were positioned upwards, counteracting the force of gravity. In contrast, for the restoration of the distal cavities, the occlusal surfaces were aligned downwards, to be parallel with the gravitational pull. After restorative procedures, each tooth was treated with 5000 thermal cycles. A solution of ammoniacal silver nitrate (AgNO3) was employed as a tracing agent. The micro-CT scans were conducted and the total volume of silver nitrate and the total volume of restorations within the relevant region of interest were calculated in "mm3" with software. Two-way ANOVA and Tukey tests were performed at a significance level of p = 0.05 with Graphpad Prism v 8.2.1 software. Results: Both gravity effect and interaction showed no statistical differences (p > 0.05). Statistically significant differences were observed in the restorative materials (p < 0.05). Conclusions: Gravitational forces do not emerge as a major factor affecting the marginal integrity of flowable bulk-fill composites in class II restorations. The chemical composition of the composites plays a more crucial role, with the XTB composite showing higher microleakage ratios compared to the others. [ABSTRACT FROM AUTHOR]

The rate of parental consent for fetal and perinatal autopsy is decreasing, whereas parents are more likely to agree to virtual autopsy by non-invasive imaging methods. Fetal and perinatal virtual autopsy needs high-resolution and good soft-tissue contrast for investigation of the cause of death and underlying trauma or pathology in fetuses and stillborn infants. This is offered by micro-computed tomography (CT), as opposed to the limited resolution provided by clinical CT scanners, and this is one of the most promising tools for non-invasive perinatal postmortem imaging. We developed and optimized a micro-CT scanner with a dual-energy imaging option. It is dedicated to post-mortem CT angiography and virtual autopsy of fetuses and stillborn infants in that the chamber can be cooled down to around 5 °C; this increases tissue rigidity and slows decomposition of the native specimen. This, together with the dedicated gantry-based architecture, attempts to reduce potential motion artifacts. The developed methodology is based on prior endovascular injection of a BaSO4-based contrast agent. We explain the design choices and considerations for this scanner prototype. We give details of the treatment of the optimization of the dual-energy and virtual mono-energetic imaging option that has been based on minimizing noise propagation and maximizing the contrast-to-noise ratio for vascular features. We demonstrate the scanner capabilities with proof-of-concept experiments on phantoms and stillborn piglets. [ABSTRACT FROM AUTHOR]

To develop Plectranthus amboinicus extract loaded Polyurethane foam dressing for burn wound healing. Plectranthus amboinicus is traditionally used as an anti-inflammatory and wound-healing agent. Its incorporation in a PU foam dressing will offer the dual benefits of foam dressing as well as the healing potential of P. amboinicus. PU foam dressings were prepared and loaded with P. ambionicus leaf extract (PAE). The dressings were prepared with varying concentrations (0.5–2%) of extract along with Toluene diisocyanate, polypropylene glycol (PPG), and liquid paraffin. The dressings were characterized by Scanning Electron Microscopy and evaluated for Moisture Vapor Transmission Rate, absorption rate, porosity, and mechanical strength followed by in vivo burn wound-healing studies in comparison to a marketed dressing. The MVTR was found to be optimum in formulations FD2-FD4 with values ranging from 2068.06 ± 0.99 to 2095.00 ± 0.25 g/m2/day. Absorption rate was found to be between 1.27 ± 0.01, 1.31 ± 0.00, and 1.30 ± 0.02 g/cm2 for formulations FD2-FD4. Formulations FD1, FD2, FD3, FD4 showed better porosity when compared to other formulations. Formulation FD4 was further characterized by micro-CT and a porosity of 46.32% was obtained. Tensile strength measurement indicated that the selected formulations were flexible enough to withstand regular handling during dressing changes. Acute dermal irritation performed on rabbits showed no irritation, erythema, eschar, and edema. In vivo wound-healing studies performed on albino wistar rats showed that the FD4 formulation has better wound healing property. Plectranthus ambionicus-loaded PU foam dressing demonstrated promising burn wound-healing potential [ABSTRACT FROM AUTHOR]

The advancement in the formulation and characterization techniques have paved the path for development of new as well as modification of existing dosage forms. The present work explores the role of micro-computed tomography (micro-CT) as advanced characterization technique for multi-layered-coated pellets to ascertain the quality of coated pellets. The work further explored in-house e-tongue technique for understanding palatability of formulation in early stages of development thus by reducing clinical taste evaluation time. The developed multi-layered-coated pellets were characterized using microscopy (optical and electron microscopy). The obtained results demonstrated formation of spherical-shaped pellets with uniform coating. The uniform coating was further confirmed by results obtained from scanning electron microscopy (SEM) and cross-sectional SEM analysis, which showed visible difference in pellet surface before and after multi-layered coating. The micro-CT results confirmed the visible demarcation of layers (drug and polymer, i.e., hydroxypropyl methylcellulose (HPMC) and eudragit (EPO)) along with uniform thickness of various layering. The dissolution study of developed pellets suggested the role of layering EPO on drug release from pellets. The e-tongue analysis proved to be an excellent tool for early prediction of taste masking of drug via multi-layered pellets and can serve as potential platform for taste masking with high specificity. The overall results suggest the suitability of developed multi-layered platform as efficient dosage form (sprinkle) in pediatric/geriatric product development. [ABSTRACT FROM AUTHOR]

The aim of this study was the development of collagen and collagen/auricular cartilage scaffolds for application in dermal regeneration. Collagen was obtained from bovine tendon by a 72 h-long treatment, while bovine auricular cartilage was treated for 24 h and divided into two parts, external (perichondrium, E) and internal (elastic cartilage, I). The scaffolds were prepared by mixing collagen (C) with the internal part (CI) or the external part (CE) in a 3:1 ratio. Differential scanning calorimetry, scanning electron microscopy (SEM) analysis, microcomputed tomography imaging (micro-CT) and swelling degree were used to characterize the scaffolds. Cytotoxicity, cell adhesion, and cell proliferation assays were performed using the cell line NIH/3T3. All samples presented a similar denaturation temperature (Td) around 48 °C, while CE presented a second Td at 51.2 °C. SEM micrographs showed superficial pores in all scaffolds and micro-CT exhibited interconnected pore spaces with porosity above 60% (sizes between 47 and 149 µm). The order of swelling was CE < CI < C and the scaffolds did not present cytotoxicity, showing attachment rates above 75%—all samples showed a similar pattern of proliferation until 168 h, whereas CI tended to decrease after this time. The scaffolds were easily obtained, biocompatible and had adequate morphology for cell growth. All samples showed high adhesion, whereas collagen-only and collagen/external part scaffolds presented a better cell proliferation rate and would be indicated for possible use in dermal regeneration. [ABSTRACT FROM AUTHOR]

Bio-Gide® is a collagen membrane routinely used in guided bone regeneration. Recent studies have shown that this collagen membrane has osteoconductive properties, meaning that it can support the growth of new bone. However, it has also been observed that the collagen membrane has areas of mineralized fibers which can occur spontaneously and independently of osteoblasts. To better understand how this works, we established a model using minced collagen membranes to reduce the active mineralization of intact collagen membranes in favor of passive mineralization. We thus compared the original intact membrane with a minced collagen membrane in a 5 mm calvarial defect model in Sprague Dawley rats. After three weeks of healing, histology and microcomputed tomography (μCT) were performed. Histological analysis confirmed the osteoconductive properties, with new bone growing inside the intact collagen membrane. However, in minced collagen membranes, the osteoconductive properties were restricted to the defect margins. Interestingly, histology revealed large mineralized areas indicating passive mineralization with no signs of bone formation. In the μCT analysis, the intact collagen membranes caused a higher median mineralized volume (1.5 mm3) compared with the minced group (0.4 mm3), but this lacked significance (p = 0.09). The μCT analysis needs to be interpreted carefully, particularly in defects filled with minced membranes, considering that the mineralized tissue may not necessarily be bone but also the result of passive mineralization. Taken together, the findings suggest that Bio-Gide® collagen membranes support bone formation while also exhibiting potential for passive mineralization. [ABSTRACT FROM AUTHOR]

In this study, we compared the material properties of linearly and sharply graded Ti6Al4V additively manufactured samples to investigate whether the more severe discontinuities caused by sharp grading can reduce performance. We performed compression testing with digital image correlation (DIC) in two loading directions for each grading design to simulate iso-stress and iso-strain conditions. We extracted the elastic stiffness, yield strength, yield strain, and energy absorption capacity of each sample. In addition, we used micro-computed tomography (micro-CT) imaging to examine the printing quality and dimensional accuracy. We found that sharply graded struts have a 12.95% increase in strut cross-sectional areas, whereas linearly graded struts produced an average of 49.24% increase compared to design. However, sharply graded and linearly graded FGL samples do not have statistically significant differences in elastic stiffness and yield strength. For the iso-strain condition, the average DIC-corrected stiffnesses for linearly and sharply graded samples were 6.15 GPa and 5.43 GPa, respectively (p = 0.4466), and the yield stresses were 290.4 MPa and 291.2 MPa, respectively (p = 0.5734). Furthermore, we confirmed different types of printing defects using micro-CT, including defective pores and disconnected struts. These results suggest that the loss of material properties caused by manufacturing defects outweighs the adverse effects of discrete-grading-induced discontinuities. [ABSTRACT FROM AUTHOR]

This study aimed to evaluate internal tooth–filling interfaces of composite fillings made using universal adhesives using micro-computed tomography (µCT). Sixty class V cavities were randomly assigned into six groups: Peak Universal etch and rinse (PER), Peak Universal self-etch (PSE), Adhese Universal etch and rinse (AER), and Adhese Universal self-etch (ASE). Two further adhesives considered gold standards were used as control groups: OptiBond FL (OER) for the etch and rinse technique and Clearfil SE for the self-etch technique (CSE). All teeth were subjected to thermomechanical loading and four-year water storage. Next, they were analyzed using µCT to investigate the internal tooth–filling interfaces. The proportions between the gap volume (GV) at the tooth–filling interface and the volume of applied composite filling (FV), between the gap and cavity volumes (CV), and between the gap volumes at the tooth–filling interface of the external (EGV) and internal (IGV) parts were calculated. Adhese Universal achieved the significantly lowest gap-to-filling- and gap-to-cavity-volume ratios for both types of etching techniques comparing to those of the Peak Universal and control groups. Significant differences between the gaps in external and internal parts of the tooth–filling interface were only noted in the control groups. Internal gap formation and development at the tooth–filling interface depend on the material as well as the type of its application. [ABSTRACT FROM AUTHOR]

Purpose: The study aimed to obtain a comprehensive 3D visualization of knee specimens, including the cruciate ligaments and corresponding femoral and tibial bone insertions using a non-destructive micro-CT method. Methods: Knee specimens were fixed in anatomical positions and chemically dehydrated before being scanned using micro-CT with a voxel size of 17.5 μm. RGBA (red, green, blue, alpha) transfer functions were applied to virtually colorize each structure. Following micro-CT scanning, the samples were rehydrated, decalcified, and trimmed based on micro-CT 3D reconstructions as references. Histological evaluations were performed on the trimmed samples. Histological and micro-CT images were registered to morphologically and densitometrically assess the 4-layer insertion of the ACL into the bone. Results: The output of the micro-CT images of the knee in extension and flexion allowed a clear differentiation of the morphologies of both soft and hard tissues, such as the ACL, femoral and tibial bones, and cartilage, and the subsequent creation of 3D composite models useful for accurately tracing the entire morphology of the ligament, including its fiber and bundle components, the trajectory between the femur and tibia, and the size, extension, and morphology of its insertions into the bones. Conclusion: The implementation of the non-destructive micro-CT method allowed complete visualization of all the different components of the knee specimens. This allowed correlative imaging by micro-CT and histology, accurate planning of histological sections, and virtual anatomical and microstructural analysis. The micro-CT approach provided an unprecedented 3D level of detail, offering a viable means to study ACL anatomy. [ABSTRACT FROM AUTHOR]

For the first time in the literature, this study validates the absorption phenomena in Multi-Jet Fusion (MJF) printed polypropylene (PP) structures through Laser Flash (LFA) and Corrected Porosity (CP) methods. The influence of process parameters such as build height and build orientation was investigated on tensile properties, crystallinity, porosity and thermophysical attributes in MJF printed PP coupons. Results showed that both crystallinity and tensile performance did not significantly vary with either location or build orientation. Interestingly, samples printed in the Z orientation showed a 35% decrease in strain, indicating that Z-oriented MJF coupons were more brittle than the flat samples (XY). Samples printed in Z orientation also possessed higher porosity and relatively lower crystallinity than the XY orientation. However, large deviations within porosity values were an obstacle to determining a suitable build chamber location for manufacturing dense samples. Therefore, a detailed investigation on porosity of printed samples using micro-CT scans and CT image analysis was necessary. Initially, poor contrast was obvious when MJF printed samples were positioned vertically in the micro-CT chamber which was mainly due to high value of horizontal intensity profile (HIP ~ 70%). Contrast in MJF samples improved significantly in the horizontal orientation (HIP ~ 40%). In parallel, the half-time and heat loss were measured in LFA to understand changes in absorption phenomena with height and orientation of the build. A direct correlation was found between LFA half-time and porosity only when the porosity correction method was implemented. Corrected porosity value was found to be inversely proportional to the heat loss of printed PP samples which indicated higher absorption for samples printed in the bottom of build chamber, XY12, whereas lower absorption was observed for less dense Z samples. Finally, heat loss phenomenon was verified using dense reference Pyroceram samples as they possess high diffusivity and low half-time and porosity compared to MJF printed samples. There is a science behind understanding the absorptivity of the MJF process which is related to the complexity of the process and is challenging to address in MJF PP samples when mixed with carbon black. The study showed that accurately determining the level of porosity is the key to validate absorption phenomena within MJF printed coupons. The contributions of this work are the investigation of the light absorption phenomena in MJF printed PP structures, and the establishment of the absorption-porosity correlation. These contributions help to predict the mechanical properties and subsequently the overall quality of the produced parts which can save cost and time in effectively utilising the MJF process. [ABSTRACT FROM AUTHOR]

This article illustrates an integrated method for characterizing hydraulic flow units in subsurface reservoirs using multiscale images and petrophysical data analysis from well cores. This method is applied to 35 meters of cores drilled from a Late Jurassic Upper Jubayla Formation outcrop in Saudi Arabia, which is analogous to the lower section of the Arab‐D reservoir. Scanning electron microscope (SEM) imagery, thin‐section petrography, and X‐ray computed tomography (CT) images of whole cores and core plugs are used to visualize and characterize these carbonate rocks across multiple length scales. Core plug porosity and permeability data are used for petrophysical characterization and deriving hydraulic flow units (HFU). The multimodal pore types associated with the HFUs and their connectivity using thin section and SEM image analysis, combined with mercury injection porosimetry are used. Whole‐core CT textures and heterogeneity logs are correlated with HFU and digital image analysis of core plugs. Whole‐core CT data prove to be a highly valuable tool for bridging the scale gap between well data and reservoir models. This methodology can be applied to datasets from a large number of wells, especially when combined with machine learning tools, allowing improved characterization of complex carbonate reservoirs. [ABSTRACT FROM AUTHOR]

The objective of this study was to compare the effects of using short and continuous fibres for repairing compression-induced tooth crown damage. Human teeth were used for the study. They were upper medial incisors and maxillary first premolars lost due to periodontal causes. The teeth were divided into two groups with Hahnenkratt and short glass fibres. Teeth compressive strength tests were carried out. Then micro-CT imaging of the teeth and their fractures obtained after compression was performed. The teeth restored with Hahnenkratt's glass fibre posts showed higher compressive strength than the teeth restored using the EverX Posterior material. The tooth's most weakened and sensitive point after endodontic treatment was the cervical area of the tooth. All cracks were parallel to the root canal. [ABSTRACT FROM AUTHOR]

Objective: To evaluate the potential of a novel synthetic carbonate apatite bone substitute (CO3Ap‐BS) on periodontal regeneration. Background: The use of various synthetic bone substitutes as a monotherapy for periodontal regeneration mainly results in a reparative healing pattern. Since xenografts or allografts are not always accepted by patients for various reasons, a synthetic alternative would be desirable. Methods: Acute‐type 3‐wall intrabony defects were surgically created in 4 female beagle dogs. Defects were randomly allocated and filled with CO3Ap‐BS (test) and deproteinized bovine bone mineral (DBBM) or left empty (control). After 8 weeks, the retrieved specimens were scanned by micro‐CT, and the percentages of new bone, bone substitute, and soft tissues were evaluated. Thereafter, the tissues were histologically and histometrically analyzed. Results: Healing was uneventful in all animals, and defects were present without any signs of adverse events. Formation of periodontal ligament and cementum occurred to varying extent in all groups without statistically significant differences between the groups. Residues of both bone substitutes were still present and showed integration into new bone. Histometry and micro‐CT revealed that the total mineralized area or volume was higher with the use of CO3Ap‐BS compared to control (66.06 ± 9.34%, 36.11 ± 6.40%; p =.014, or 69.74 ± 2.95%, 42.68 ± 8.68%; p =.014). The percentage of bone substitute surface covered by new bone was higher for CO3Ap‐BS (47.22 ± 3.96%) than for DBBM (16.69 ± 5.66, p =.114). Conclusions: CO3Ap‐BS and DBBM demonstrated similar effects on periodontal regeneration. However, away from the root surface, more new bone, total mineralized area/volume, and higher osteoconductivity were observed for the CO3Ap‐BS group compared to the DBBM group. These findings point to the potential of CO3Ap‐BS for periodontal and bone regeneration. [ABSTRACT FROM AUTHOR]

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Microwave radiation in the production of lightweight aggregates, it has not yet been thoroughly explored. Therefore, the main goal of the research is to analyze the possibility of using various types of waste, such as waste rock (0–30 %) and glass cullet (10 %), fly ash (50–80 %) and waste solution using different microwave temperature (1000, 1050, 1100 °C) in order to obtain lightweight aggregates. Above factors and, chemical and mineral composition of the waste materials, affect the microstructure and physico-mechanical parameters of the aggregates.). The thermophysical behavior (DTG/TG), grain composition, chemical composition (XRF), phase composition (XRD) and microstructure (SEM, μ-CT) were analyzed. The microstructure, as well as the related characteristics of aggregates, i.e. water absorption, density, and compressive strength, depend on the sintering temperature, chemical composition and mineral waste raw material. The best properties were obtained for a raw material mixture containing 80 % coal fly ash, 10 % glass cullet, 10 % clay and 48 g of the waste solution (per 100 g of raw mixture), sintered in the microwave at 1100 °C. The aggregates were characterized by water absorption of 6.96 %, bulk of 1.70 g/cm3, loose bulk density 0.60 g/cm3, and compressive strength 5.95 MPa. Closed porosity determined by micro-CT was 8.41 %, open - 0.24 %, sphericity 0.62. The total porosity depends on the loose bulk density and particle density of lightweight aggregates. Furthermore, washout tests meet environmental requirements. The proposed technology, moreover, enables a short time of thermal processing of raw materials, and thus energy savings and a sustainable approach to the production of green pellets. Thanks to the high strength, the obtained aggregates can replace commercial aggregates. Flow chart for the manufacture of the artificial aggregates. [Display omitted] [ABSTRACT FROM AUTHOR]

Global oil and gas resources are declining continuously, and sustainable development has become a common challenge worldwide. In terms of environmental protection and economic benefits, the application of microemulsions for enhanced oil recovery often requires fewer chemical agents, showing distinct advantages. This paper analyzes the application prospects and trends of middle-phase microemulsions in tertiary oil recovery. The properties of middle-phase microemulsions are introduced, and an overview of the historical development, theoretical framework, influencing factors, and preparation methods of emulsions are provided. From the perspective of oil displacement systems, this paper reviews the selection and characterization methods of emulsions, as well as the interaction mechanisms between emulsions and reservoirs, proposing future research directions. The focus of the paper is on the evaluation and characterization of emulsions, the mechanisms of micro-oil displacement, and the application of advanced CT scanning technology, which gives a new understanding of wettability changes, capillary forces, and miscible solubilization processes, contributing to the reduction in displacement costs and the improvement of economic benefits. In conclusion, the middle-phase microemulsion flooding technique can significantly enhance oil recovery through the comprehensive action of various mechanisms and has been widely used in oil field development. [ABSTRACT FROM AUTHOR]

X-ray-based computed tomography is a well established technique for determining the three-dimensional structure of an object from its twodimensional projections. In the past few decades, there have been significant advancements in the brightness and detector technology of tomography instruments at synchrotron sources. These advancements have led to the emergence of new observations and discoveries, with improved capabilities such as faster frame rates, larger fields of view, higher resolution and higher dimensionality. These advancements have enabled the material science community to expand the scope of tomographic measurements towards increasingly in situ and in operando measurements. In these new experiments, samples can be rapidly evolving, have complex geometries and restrictions on the field of view, limiting the number of projections that can be collected. In such cases, standard filtered back-projection often results in poor quality reconstructions. Iterative reconstruction algorithms, such as model-based iterative reconstructions (MBIR), have demonstrated considerable success in producing high-quality reconstructions under such restrictions, but typically require highperformance computing resources with hundreds of compute nodes to solve the problem in a reasonable time. Here, tomoCAM, is introduced, a new GPUaccelerated implementation of model-based iterative reconstruction that leverages non-uniform fast Fourier transforms to efficiently compute Radon and back-projection operators and asynchronous memory transfers to maximize the throughput to the GPU memory. The resulting code is significantly faster than traditional MBIR codes and delivers the reconstructive improvement offered by MBIR with affordable computing time and resources. tomoCAM has a Python front-end, allowing access from Jupyter-based frameworks, providing straightforward integration into existing workflows at synchrotron facilities. [ABSTRACT FROM AUTHOR]

Recently, a paste-type premixed calcium silicate-based mineral trioxide aggregate (MTA) product that quickly solidifies through a pozzolanic reaction was introduced to replace existing MTA, which has the disadvantage of a long setting time. In this study, we evaluated the effect of moisture content in the root canal on the setting time of premixed calcium silicate-based MTA in a simulated root canal environment using Endoseal MTA and Well-Root ST, among commercially available products. The setting time was measured according to ISO 6876/2012. A mold made using grades 2, 3, and 4 dental gypsum according to the classification of ISO 6873/2013 was used to reproduce the difference in moisture environment. Differences in moisture content were measured using micro-computed X-ray tomography (micro-CT). The micro-CT results showed that the moisture content was the highest and lowest in the grade 2 and 4 gypsum molds, respectively. Moreover, the setting time indicated by the manufacturer was the shortest for the grade 2 gypsum mold. Hence, the differences in moisture content significantly affect the setting time of MTA. This result can help set future experimental conditions and develop premixed calcium silicate-based MTA products. [ABSTRACT FROM AUTHOR]

Background The incidence of osteonecrosis of the femoral head (ONFH) in people with human immunodeficiency virus (HIV) is 10–100 times higher than that in the general population. However, the specific bone microstructure and extent of damage within the femoral head in PWH are still unclear. Methods Femoral head samples were obtained by total hip arthroplasty, micro–computed tomography (micro-CT) was employed to investigate the microstructure of trabecular bone across 4 representative regions within necrotic femoral heads, and quantitative analysis was performed. Results On general observation, different degrees of degenerative cartilage, fibrocartilage, hyperplastic bone, and exposed bone were presented alternately, with a "map-like" appearance. On micro-CT, compared with the normal and necrotic areas, the bone volume/tissue volume and bone mineral density of the sclerotic areas were significantly increased, the number of trabeculae was significantly increased, and the gap was smaller (P <.05). There was no significant difference in trabecular thickness among the groups (P <.05). Conclusions The systemic immune syndrome caused by HIV itself may interfere with the normal metabolism of bone, including osteoblasts and osteoclasts, and thus participate in HIV-related ONFH. [ABSTRACT FROM AUTHOR]

Background: The distinction between where the pulp chamber ends and the root canal system begins is poorly defined within the existing literature. Methods: This paper aimed to describe a range of accurate methods to define the transition from pulp chamber to root canal in different first molar root morphologies using micro-focus computed tomography (micro-CT). Methods: The sample consisted of 86 mandibular and 101 maxillary first molars from the skeletal collections housed in the Department of Anatomy and Histology of the Sefako Makgatho Health Sciences University and the Pretoria Bone Collection. A stepwise approach using the cemento–enamel junction (CEJ) and dedicated landmarks was followed to create an automated cross-sectional slice. Results: Transition from pulp chamber to root canal could be accurately determined on maxillary and mandibular teeth. The occurrence of two separate roots in mandibular molars was 97.7%, with the remaining 2.3% having an additional disto-lingual root, with no mandibular molars displaying fused roots. In the maxillary molars, 92.1% had three separate roots and 7.9% displayed root fusion. Within this group, one tooth displayed a C-shaped root canal configuration and one a mesotaurodont-type morphology. Conclusion: The suggested methodology to determine orifice location was found to be appropriate in all morphological types. [ABSTRACT FROM AUTHOR]

Bentonite–sand mixtures have been proposed as the potential engineered barrier materials because of their low permeability and strong sealing ability under hydrated repository conditions. However, the mixtures may gradually lose water and shrink, which could compromise the engineering integrity of the barrier, under the environmental conditions of the repository where there is a continuous release of radiant heat from nuclear waste. To investigate the shrinkage characteristics of compacted bentonite–sand mixtures, we carry out laboratory drying tests by preparing samples with varying initial dry densities, water contents, and sand contents. The fabric of sand particles is characterized through the quantitative analysis of micro-CT scanning results. Experimental results reveal that both initial compaction condition and sand content significantly influence the shrinkage behaviors of bentonite–sand mixtures. Both shrinkage strain and shrinkage coefficient of the specimens decrease with the increasing initial dry density and the decreasing initial water content. Generally, the development of suction during drying is responsible for the volume shrinkage. The shrinkage and water retention capacities reduce with the increasing sand content in the mixture. Moreover, micro-CT results reveal that, with higher amount of sand inclusions, the mixture presents higher sand particle contact density. When sand content exceeds 30%, these sand particles tend to form sand skeletons that provide high inter-particle friction forces and limit the further shrinkage of mixture. This study is expected to improve the basic understanding of the underlying shrinkage mechanisms of bentonite–sand mixtures and help guide the future design of nuclear waste repository. [ABSTRACT FROM AUTHOR]

Objectives: This study aimed to determine the degree of similarity and symmetry in the anatomy of contralateral mandibular incisors. Three-dimensional (3D) models of extracted teeth were obtained from microtomography (micro-CT) scans. Qualitative and quantitative assessments of the morphology and comparison of contralateral pairs were made. The null hypothesis was that contralateral mandibular incisors could not be considered identical in simple morphometric measurements. Methods: Sixty pairs of mandibular incisors were extracted from 30 patients and scanned with micro-CT. Virtual models of the cemento-enamel junction to the root apex were rendered. Parameters such as length, canal width, dentinal thicknesses, tortuosity, centerline length, accessory canals, root canal configurations, and root canal orifice cross-sections were used to compare the teeth. Width and thickness comparisons between paired teeth in the same individual were made by paired t-test (Wilcoxon signed-rank test for variables not normally distributed). An online randomization tool generated randomized pairs (independent of the individual/patient). Subsequently, an unpaired t-test (or Mann–Whitney U test for non-normally distributed parameters) and a correlation analysis were conducted. Canal configurations were classified according to preexisting classification schemes. The number and location of accessory canals and apical foramina were registered and compared. Results: Utilizing advanced imaging techniques and quantitative analyses, our study establishes that contralateral mandibular incisors exhibit a remarkable degree of symmetry in multiple morphological parameters, including length, canal width, and dentinal thicknesses. The apical third showed a high degree of inter-variability for the contralateral pairs. The rigorous statistical analysis of the normalized parameters by Z-score showed no statistically significant differences between the contralateral mandibular incisors. Comparisons between central and lateral teeth revealed differences in root length but no significant disparity in the distribution of accessory canals. Central teeth, on average, were longer, while accessory canals were distributed relatively evenly between central and lateral teeth. Conclusions: The findings of this study further establish the significant similarities between contralateral mandibular incisors, reinforcing their suitability as a reliable substrate for root canal comparison studies. Clinical relevance: The absence of statistically significant differences between contralateral pairs in normalized parameters underscores their potential as a reliable reference point for root canal comparison studies in clinical dentistry. Furthermore, our findings emphasize the importance of individualized treatment planning, considering the natural symmetry in mandibular incisors to enhance clinical decision-making. This research contributes valuable insights to the field of endodontics, offering a standardized approach to sample selection and enriching the understanding of dental anatomy. [ABSTRACT FROM AUTHOR]

Tsuga (hemlock) is a small genus of 10 extant species in the Pinaceae, with a disjunct distribution in East Asia and eastern and western North America. Reliable species‐level identification of Tsuga fossils depends on the discovery of seed cones with intact bracts, but such cones are rare in the fossil record. Here we describe a new fossil species of hemlock as T. weichangensis sp. nov. based on exquisitely preserved seed cones with nearly complete bracts from the Lower Miocene of Weichang, Hebei Province, North China. This fossil species displays a mosaic of characters between Tsuga and Nothotsuga. The well‐developed and slightly exserted bract scales of T. weichangensis are reminiscent of Nothotsuga, but other characters, such as nonleaved peduncles and tongue‐shaped bract scales, in addition to monosaccate pollen found at the same fossil locality, suggest an affinity closer to Tsuga. Cladistic analysis based on 15 morphological characters and a molecular backbone constraint supports the assignment of these fossil cones to Tsuga rather than Nothotsuga, and places the fossil species of T. weichangensis in an unresolved polytomy within the genus Tsuga. The occurrence of Tsuga seed cone fossils indicate the paleoclimate in the Miocene of Weichang was warmer and more humid than today's climate, which is consistent with the paleoclimate reconstructed by paleopalynology. [ABSTRACT FROM AUTHOR]

• The effectiveness of published stab wound analysis was examined. • Qualitative and quantitative techniques to assess kerf mark properties were considered. • A standardised decision-making approach to kerf mark profile acquisition was developed. Stab wound analysis is a relatively new field of study in forensic science, and there is currently much debate regarding the effectiveness of the analysis due to a lack of validation studies. Furthermore, the underlying viewpoints on the success of stab wound analysis vary. Examination of cut marks, for example, can reveal a variety of characteristics which can be used to determine the type of weapon that was used to inflict them. However, published studies are not consistent when identifying knife blade characteristics, instead considering a wide variety of morphological aspects and their potential value in forensic scenarios. The existing research methodology is therefore inadequate to reliably inform in such contexts, and future experimental design should be influenced by the conditional variance in stabbings in order to provide reliable findings. The research presented here takes a systematic approach to the problem, compiling the published literature (up to September 2023) on the use of different imaging methods applied to stab wound examination to create a taxonomy to examine trends in methodological approaches in both research and investigative settings. This approach identified that published studies could be classified as either morphological or morphometrical, and further sub-classified based on their degree of success and the findings reached. This emphasises the importance of prioritising research into mark data, and the need for a multi-technique, multi-disciplinary approach. A decision tree was created to illustrate which mark attributes should be studied for which purpose, and using which imaging method(s). Furthermore, the research presented identifies two key areas in stab wound research which should be the focus of standardisation efforts, namely methodological procedures and mark characteristic collection. Knife markings are difficult to interpret, but further research and standardisation of kerf mark analysis, as highlighted here, will improve the efficiency and reliability of both forensic investigations and future experimental studies. [ABSTRACT FROM AUTHOR]

Variations in anatomic apex and apical foramina of root canals are common in different teeth types. The sophisticated 3D micro-CT aids researchers in investigating the apical morphology, such as the apical foramen (AF) and constriction (AC). To measure the length between the foramen and the apex and the physiological length to the apex, in lower premolar teeth. Furthermore, a measurement was conducted on the average length from the foramen to the apex of the lower premolar teeth with respect to the presence of clinical and non-clinical signs. A total of 80 lower premolar teeth were selected based on the inclusion criteria. All samples were scanned using micro-CT ZEISS X-Radia (17 µm), and the datasets were analysed. The root canal AF and AC were reconstructed using Drishti software (V3.0) by utilising a 4-digit system code in millimetres (mm). More than half of the lower premolars (n = 47, 58.8 %) demonstrated a clinical signs presentation, while the rest had a non-clinical sign (n = 33, 41.3 %). There was a significant difference (p = 0.013) between the non-clinical and clinical groups in the mean length from the apical foramen to the apex (AFA) at 0.59 mm and 0.47 mm, respectively. Meanwhile, the non-clinical and clinical mean length from apical constriction to apex (ACA) were 0.75 mm and 0.73 mm, and the mean length from AF to AC (AFC) was 0.48 mm and 0.53 mm, respectively. Nonetheless, no significant differences were detected between the ACA and AFC of non-clinical and clinical groups. The clinical signs presentation observed in lower premolars mainly affected the apical morphology, particularly the position and length of the root AF, and less so the AC position and length. Therefore, the information is useful for clinical purposes. [ABSTRACT FROM AUTHOR]

Purpose: The study's objective was to evaluate the efficiency of various irrigation activation methods for removing gutta-percha and sealer using Micro-CT and SEM after retreatment with rotary files. Methods: Twenty-one permanent single-rooted teeth that were extracted and had a single canal were decoronated to a length of 16 mm. AH Plus sealer was used for obturating the root canals. Following obturation, Micro-CT scanning was carried out (S1). Another Micro-CT scan was performed following the elimination of the original filling material using ProTaper Universal retreatment files (S2). Next, each of the 21 samples was divided into three groups (n = 7): Group 1: XP-Endo Finisher (XPF); Group 2: Passive Ultrasonic Irrigation (PUI); Group 3: SWEEPS. Subsequent irrigation activation technique by one of each system was followed by the final Micro-CT scanning (S3). After calculating the remnant volume of the filling material, a single specimen was examined under a scanning electron microscope for every group. Statistical evaluation was accomplished utilizing the Kruskal-Wallis and Shapiro-Wilk tests. Results: After analyzing the samples, S1 and S2 scanning results revealed no statistically significant differences among the three groups (p > 0.05). Furthermore, no significant difference in the final volume of residual filling material (S3) between the three groups was found statistically. Conclusion: In summary, XPF, PUI, and SWEEPS techniques are equally efficient at removing remnant filling materials after conventional retreatments. [ABSTRACT FROM AUTHOR]

In practically every industry, mixing is a fundamental process, yet its 3D analysis is scarce in the literature. High-resolution computed tomography (micro-CT) is the perfect X-ray imaging tool to investigate the mixing of granular materials. Other than qualitative analysis, 3D micro-CT images provide an opportunity for quantitative analysis, which is of utmost importance, in terms of efficiency (time and budget) and environmental impact of the mixing process. In this work, lacunarity is proposed as a measure of mixing. By the lacunarity calculation on the repeated micro-CT measurements, a temporal description of the mixing can be given in three dimensions. As opposed to traditional mixing indices, the lacunarity curve provides additional information regarding the spatial distribution of the grains. Discrete element method simulations were also performed and showed similar results to the experiments. [ABSTRACT FROM AUTHOR]

Main conclusion: PROG1 is necessary but insufficient for the main culm inclination while TAC1 partially takes part in it, and both genes promote tiller inclination in Asian wild rice. Asian wild rice (Oryza rufipogon), the ancestor of cultivated rice (O. sativa), has a prostrate architecture, with tillers branching from near the ground. The main culm of each plant grows upward and then tilts during the vegetative stage. Genes controlling tiller angle have been reported; however, their genetic contributions to the culm movement have not been quantified. Here, we quantified their genetic contributions to angular kinematics in the main culm and tillers. For the main culm inclination, one major QTL surrounding the PROG1 region was found. In cultivated rice, tillers firstly inclined and lately rose, while it kept inclining in wild rice. It was suggested that PROG1 affected the tiller elevation angle in the later kinematics, whereas TAC1 was weakly associated with the tiller angle in the whole vegetative stage. Micro-computed tomography (micro-CT) suggested that these angular changes are produced by the bending of culm bases. Because near-isogenic lines (NILs) of wild rice-type Prog1 and Tac1 alleles in the genetic background of cultivated rice did not show the prostrate architecture, the involvement of another gene(s) for inclination of the main culm was suggested. Our findings will not only contribute to the understanding of the morphological transition during domestication but also be used in plant breeding to precisely reproduce the ideal plant architecture by combining the effects of multiple genes. [ABSTRACT FROM AUTHOR]

Despite the dominant role of bone mass in osteoporotic fractures, aging bone tissue properties must be thoroughly understood to improve osteoporosis management. In this context, collagen content and integrity are considered important factors, although limited research has been conducted on the tensile behavior of demineralized compact bone in relation to its porosity and elastic properties in the native mineralized state. Therefore, this study aims (i) at examining the age-dependency of mineralized bone and collagen micromechanical properties; (ii) to test whether, and if so to which extent, collagen properties contribute to mineralized bone mechanical properties.Two cylindrical cortical bone samples from fresh frozen human anatomic donor material were extracted from 80 proximal diaphyseal sections from a cohort of 24 female and 19 male donors (57 to 96 years at death). One sample per section was tested in uniaxial tension under hydrated conditions. First, the native sample was tested elastically (0.25 % strain), and after demineralization, up to failure. Morphology and composition of the second specimen was assessed using micro-computed tomography, Raman spectroscopy, and gravimetric methods. Simple and multiple linear regression were employed to relate morphological, compositional, and mechanical variables with age and sex.Macro-tensile properties revealed that only elastic modulus of native samples was age dependent whereas apparent elastic modulus was sex dependent (p

Silicosis is a progressive pulmonary fibrosis disease caused by long-term inhalation of silica. The early diagnosis and timely implementation of intervention measures are crucial in preventing silicosis deterioration further. However, the lack of screening and diagnostic measures for early-stage silicosis remains a significant challenge. In this study, silicosis models of varying severity were established through a single exposure to silica with different doses (2.5mg/mice or 5mg/mice) and durations (4 weeks or 12 weeks). The diagnostic performance of computed tomography (CT) quantitative analysis was assessed using lung density biomarkers and the lung density distribution histogram, with a particular focus on non-aerated lung volume. Subsequently, we developed and evaluated a stacking learning model for early diagnosis of silicosis after extracting and selecting features from CT images. The CT quantitative analysis reveals that while the lung densitometric biomarkers and lung density distribution histogram, as traditional indicators, effectively differentiate severe fibrosis models, they are unable to distinguish early-stage silicosis. Furthermore, these findings remained consistent even when employing non-aerated areas, which is a more sensitive indicator. By establishing a radiomics stacking learning model based on non-aerated areas, we can achieve remarkable diagnostic performance to distinguish early-stage silicosis, which can provide a valuable tool for clinical assistant diagnosis. This study reveals the potential of using non-aerated lung areas as a region of interest in stacking learning for early diagnosis of silicosis, providing new insights into early detection of this disease.

Recently, new insights have been gained from the ophiuroid skeleton that were instrumental in the inference of a new phylogeny. The so far least studied ossicles are the adradial and abradial genital plates and the radial shields, which articulate with each other and support the genital slit and disc. In addition, the inner sides of the oral shields and madreporites have never been examined in detail. The present study utilized SEM, micro-CT and digital photography to document and examine these structures in 57 species from 28 of the currently accepted 34 families of Ophiuroidea. Early ontogeny and fossils were also considered. Previously, mainly the articular structures had been analysed, but the overall shape of the genital plates was here found to hold important phylogenetic signals. A long-neglected ossicle was re-discovered and studied in detail for the first time, here named the oral genital plate. It was recognized in all Ophintegrida, but was found to be absent in all Euryophiurida. The oral genital plate articulates with the oral shield and supports the proximal part of the genital slit wall. Abradial and oral genital plates were found to be absent in species that lack genital slits, but the adradial genital plate was always present. Numerous new morphological characters with potential phylogenetic signals were identified, described and figured in detail. A pre-existing character matrix was extended and revised with these new data, as well as with recently revised data on oral papillae, and a Bayesian phylogenetic analysis was performed. This phylogeny largely agrees with the current molecular hypothesis, but some branches were not supported.

ABSTRACT: Despite the acknowledged significance of nutrition in bone development, effects of methionine (Met) and cysteine (Cys) on bone quality remain under-researched, particularly during Eimeria challenge. We investigated the effects of different supplemental Met to Cys ratios (MCR) on bone quality of broilers under Eimeria challenge. A total of 720 fourteen-day old Cobb500 broilers were allocated into a 5 × 2 factorial arrangement. Five diets with Met and Cys supplemented at MCR of 100:0, 75:25, 50:50, 25:75, and 0:100 were fed to the birds with or without Eimeria challenge. Body composition was measured by dual energy x-ray absorptiometry, and the femur bone characteristics were assessed by microtomography. Data were analyzed by two-way ANOVA and orthogonal polynomial contrast. The results reaffirmed the detrimental effects of Eimeria challenge on bone quality. On 9 d post inoculation (DPI), significant interaction effects were found for whole body bone mineral content (BMC), lean tissue weight, and body weight (P < 0.05); in the nonchallenged group (NCG), these parameters linearly decreased as MCR decreased (P < 0.05). In the challenged group (CG), body weight and lean tissue weight were unaffected by MCR, and BMC linearly increased as MCR decreased (P < 0.05). For the cortical bone of femoral metaphysis on 6 DPI, bone mineral density (BMD) linearly increased as MCR decreased (P < 0.05). Bone volume to tissue volume ratio (BV/TV) in the CG linearly increased as MCR decreased (P < 0.05). On 9 DPI, BMC and TV linearly increased as MCR decreased (P < 0.05) in the NCG. BMD and BV/TV changed quadratically as MCR decreased (P < 0.05). For the trabecular bone of femoral metaphysis on 9 DPI, BV/TV, and trabecular number linearly increased as MCR decreased (P < 0.05) in the NCG. For the femoral diaphysis, BV, TV, BMC on 6 DPI, and BMD on 9 DPI linearly increased as MCR decreased (P < 0.05). In conclusion, this study showed that both Eimeria challenge and varying supplemental MCR could influence bone quality of broilers.

Abstract Comparative anatomy is an important tool for investigating evolutionary relationships among species, but the lack of scalable imaging tools and stains for rapidly mapping the microscale anatomies of related species poses a major impediment to using comparative anatomy approaches for identifying evolutionary adaptations. We describe a method using synchrotron source micro‐x‐ray computed tomography (syn‐μXCT) combined with machine learning algorithms for high‐throughput imaging of Lepidoptera (i.e., butterfly and moth) eyes. Our pipeline allows for imaging at rates of ~15 min/mm3 at 600 nm3 resolution. Image contrast is generated using standard electron microscopy labeling approaches (e.g., osmium tetroxide) that unbiasedly labels all cellular membranes in a species‐independent manner thus removing any barrier to imaging any species of interest. To demonstrate the power of the method, we analyzed the 3D morphologies of butterfly crystalline cones, a part of the visual system associated with acuity and sensitivity and found significant variation within six butterfly individuals. Despite this variation, a classic measure of optimization, the ratio of interommatidial angle to resolving power of ommatidia, largely agrees with early work on eye geometry across species. We show that this method can successfully be used to determine compound eye organization and crystalline cone morphology. Our novel pipeline provides for fast, scalable visualization and analysis of eye anatomies that can be applied to any arthropod species, enabling new questions about evolutionary adaptations of compound eyes and beyond.

Aim: The study aimed to evaluate the shaping characteristics of ProTaper Ultimate (Dentsply Sirona) and BlueShaper (Zarc4Endo) using microcomputed tomographic imaging technology (micro-CT).Methods: Thirty mesial and distal canals of extracted second mandibular molars with similar anatomy were selected and scanned pre- and postoperatively by micro-CT scanning (SkyScan 1172, Bruker micro-CT) with a voxel size of 11 μm and shaped with two different procedures: ProTaper Ultimate (Group 1: n = 15) and BlueShaper (Group 2: n = 15) were used following the manufacturer’s recommendations up to final 30 apical size. Irrigation was ensured in all groups with 5.25% NaOCl and 10% EDTA during instrumentation. Canal transportation, the volume of removed dentin, and area of untreated surface were matched with preoperative values with Bruker CT Analyser (Bruker micro-CT). Data were statistically analyzed using Student’s t-test.Results: No significant differences were observed between Group 1 and Group 2 in terms of canal transportation, volume of removed dentin, and untreated surfaces. A significant difference (p < 0.05) in the amount of untreated surfaces was found between the mesial and distal canals in both groups.Conclusion: Shaping procedures with both ProTaper Ultimate and BlueShaper demonstrated similar canal enlargement volumes and similar canal transportation without evidence of significant preparation errors. Nevertheless, a high amount of untreated surface was reported for both systems, particularly in distal canals.

The interfacial transition zone (ITZ) in cement-based composites with aggregates is widely recognized as the most vulnerable component, greatly affecting their physical and mechanical properties. In this study, the effect of the ITZ characteristics on the mechanical behavior of cement mortar using its actual microstructure was investigated with image-based numerical approaches in this study. The volumetric information of the ITZ of the mortar sample was obtained and characterized from high-resolution synchrotron micro-computed tomography (micro-CT) images. The mechanical properties of the mortar were evaluated using the finite element method, based on the components characteristics represented by grayscale information of the micro-CT images. It was found that the adhesion between the aggregates and binder was well replicated in the simulation with the multiple-phase model, based on the gradient characteristics and properties of the ITZ. Considering the ITZ thickness according to the aggregates size, a more realistic crack propagation trend can be observed. The obtained results confirmed the significant influence of the ITZ thickness and phase distribution characteristics on the mechanical properties of cement mortars, emphasizing the importance of considering ITZ phases for accurate material analysis.

AbstractThis study explores the impact of chronic caffeine intake on collagen deposition in long bones and correlates it with morphometric and metabolic alterations in Wistar rats. Animals had either regular drinking water (Control group) or caffeinated drinking water in a 300 mg/L dose (Caffeine group) for 12 wk. Using Picro-Sirius red collagen staining, cortical (78.07 ± 1.27 vs 82.12 ± 1.22) and trabecular (74.29 ± 1.09 vs 77.97 ± 1.03) collagen percentages in the caffeine group were markedly (p = 0.0448 and p = 0.0351; respectively) lower than the control group. Caffeine intake induced apparent alterations in the microstructure of cortical diaphysis and femoral head trabecular density as determined by micro-CT. In caffeinated animals, levels of bone-related metabolic biomarkers including BALP (1.41 ± 0.06 vs 1.12 ± 0.07), CTX (313.5 ± 20.78 vs 224.3 ± 27.19), DPD (11.61 ± 0.72 vs 9.26 ± 0.62), BGP (10.35 ± 0.43 vs 9.14 ± 0.34), and TRAP (4.34 ± 0.08 vs 4.01 ± 0.11) were increased (p = 0.0136, p = 0.0262, p = 0.0343, p = 0.0571, and p = 0.0461; respectively) as compared to control animals. Except for BALP (p = 0.0175) and DPD (p = 0.0423), the Pearson correlation between collagen bone deposition and microstructures and metabolic changes was not statistically significant. Collectively, chronic caffeine consumption restricts cortical and trabecular collagen deposition in long bones leading to microstructure and metabolic alterations.

Abstract We have recently studied northern Finnish archaeological textiles extensively using computed tomography (CT) imaging. These textiles have been found in inhumation burials from the Late Medieval church of Valmarinniemi in Keminmaa and the Postmedieval church of Haukipudas. In this article we discuss the advantages and limitations of CT imaging based on three case studies. Based on the research objectives and the size of studied items, we utilised three different CT scanners: clinical systems and micro- and nano-scale X-ray microscopes. We were able to visualise a child’s coffin and a doll inside, which is a larger scale sample. We were also able to study and reconstruct the complicated structure of a tablet-woven band, as well as identifying individual fibres when examining smaller textile samples with submicron resolution. Even though we observed some limitations in the image quality, we conclude that computed tomography has great potential in the research of archaeological textiles in both 3D and cross-sections and is often easier and more informative than conventional microscopic or other archaeological methodologies.

This study evaluated the changes in physicochemical properties and appearance quality of long-grain rice during the grinding process using image technologies and aimed to provide reference for future research. The brown rice milling process was divided into three stages, and flatbed scanning, scanning electron microscopy (SEM), X-ray micro-computed tomography (micro-CT), low-field nuclear magic resonance (LF-NMR), and headspace–gas chromatography–ion mobility spectrometry (HS–GC–IMS) were employed to examine the physicochemical and volatile properties of the samples. Results revealed a continuous increase in the degree of milling, with a broken rice rate and a whiteness value increasing by 50.84% and 21.13%, respectively, compared with those during the initial stage; dietary fiber and vitamin B1 contents were reduced by 54.41% and 66.67%, respectively. The image results visualized showed that the cortex of brown rice was gradually peeled off with the increase in milling degree; the cortical thickness was gradually reduced, the endosperm was gradually exposed, and the surface was smoother and shinier. T2 populations exhibited a shift toward longer relaxation times, followed by a decrease in relaxation time during the milling process. Additionally, 31 target compounds impacting rice flavor, mainly ketones, alcohols, and esters, were identified, and the concentration of volatile substances in the B region decreased with the reduction in the bran layer; the concentration of volatile substances in the C region provided rice flavor, which increased with the milling process. This study showed changes in the physicochemical properties and appearance quality of long-grain brown rice during milling. Furthermore, the use of various image processing techniques offers significant insights for optimizing processing parameters and enhancing overall quality and taste.

Understanding the microstructure of fibrous tissues, like ligaments, is crucial due to their nonlinear stress-strain behavior from unique fiber arrangements. This study introduces a new method to analyze the relationship between the microstructure and function of anterior cruciate ligaments (ACL). We tested the procedure on two ACL samples, one from a healthy individual and one from an osteoarthritis patient, using a custom tensioning device within a micro-CT scanner. The samples were stretched and scanned at various strain levels (namely 0%, 1%, 2%, 3%, 4%, 6%, 8%) to observe the effects of mechanical stress on the microstructure. The micro-CT images were processed to identify and map fibers, assessing their orientations and volume fractions. A probabilistic mathematical model was then proposed to relate the geometric and structural characteristics of the ACL to its mechanical properties, considering fiber orientation and thickness. Our feasibility test indicated differences in mechanical behavior, fiber orientation, and volume distribution between ligaments of different origins. These indicative results align with existing literature, validating the proposed methodology. However, further research is needed to confirm these preliminary observations. Overall, our comprehensive methodology shows promise for improving ACL diagnosis and treatment and for guiding the creation of tissue-engineered grafts that mimic the natural properties and microstructure of healthy tissue, thereby enhancing integration and performance in biomedical applications.