319 results on '"Michael A. Lane"'
Search Results
202. Management development programs: The effects of management level and corporate strategy
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Gerald L. Blakely, Michael S. Lane, and Cindy Lee Martinec
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Organizational Behavior and Human Resource Management ,Entrepreneurship ,ComputingMilieux_THECOMPUTINGPROFESSION ,Management development ,Ethical decision ,Middle management ,Management ,Arts and Humanities (miscellaneous) ,Enterprise relationship management ,Retrenchment ,Strategic management ,Business ,Marketing ,Senior management - Abstract
In this study of 155 organizations, the relationship between management development programs, managerial level, and organizational strategy was examined. Limited support was established for Katz's theory about skill requirements for managers (1974). Greater emphasis was placed on technical skills at lower management levels and on entrepreneurial skills at senior management levels. A relationship between corporate strategy and the focus of management development programs also was found. Although organizations with growth strategies focused on many management development areas to a greater extent than did organizations with stability or retrenchment strategies, there were no differences in the focus on ethical decision making or technical-skills training between the three strategies. Implications for management and for future research are also discussed.
- Published
- 1994
203. Respiratory function following bilateral mid-cervical contusion injury in the adult rat
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Michael A. Lane, Krystal Salazar, Kun-Ze Lee, David C. Bloom, Barbara E. O'Steen, David D. Fuller, and Paul J. Reier
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Diaphragm ,Article ,Lumbar enlargement ,White matter ,Rats, Sprague-Dawley ,Developmental Neuroscience ,Neuroplasticity ,medicine ,Animals ,Respiratory function ,Respiratory system ,Spinal cord injury ,Spinal Cord Injuries ,Motor Neurons ,business.industry ,Electromyography ,Respiration ,Anatomy ,Recovery of Function ,Spinal cord ,medicine.disease ,Hyperintensity ,Rats ,medicine.anatomical_structure ,Neurology ,Spinal Cord ,Cervical Vertebrae ,Female ,business - Abstract
The consequences of spinal cord injury (SCI) are often viewed as the result of white matter damage. However, injuries occurring at any spinal level, especially in cervical and lumbar enlargement regions, also entail segmental neuronal loss. Yet, the contributions of gray matter injury and plasticity to functional outcomes are poorly understood. The present study addressed this issue by investigating changes in respiratory function following bilateral C(3)/C(4) contusion injuries at the level of the phrenic motoneuron (PhMN) pool which in the adult rat extends from C(3) to C(5/6) and provides innervation to the diaphragm. Despite extensive white and gray matter pathology associated with two magnitudes of injury severity, ventilation was relatively unaffected during both quiet breathing and respiratory challenge (hypercapnia). On the other hand, bilateral diaphragm EMG recordings revealed that the ability to increase diaphragm activity during respiratory challenge was substantially, and chronically, impaired. This deficit has not been seen following predominantly white matter lesions at higher cervical levels. Thus, the impact of gray matter damage relative to PhMNs and/or interneurons becomes evident during conditions associated with increased respiratory drive. Unaltered ventilatory behavior, despite significant deficits in diaphragm function, suggests compensatory neuroplasticity involving recruitment of other spinal respiratory networks which may entail remodeling of connections. Transynaptic tracing, using pseudorabies virus (PRV), revealed changes in PhMN-related interneuronal labeling rostral to the site of injury, thus offering insight into the potential anatomical reorganization and spinal plasticity following cervical contusion.
- Published
- 2011
204. The effect of changes in land use on nitrate concentration in water supply wells in southern Chester County, Pennsylvania
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Christina L. Ruble, Michael E. Lane, and Paul White
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Conservation of Natural Resources ,Water supply ,Aquifer ,Management, Monitoring, Policy and Law ,engineering.material ,chemistry.chemical_compound ,Nitrate ,Agricultural land ,Water Supply ,General Environmental Science ,Hydrology ,geography ,geography.geographical_feature_category ,Nitrates ,Land use ,business.industry ,Environmental engineering ,General Medicine ,Contamination ,Pennsylvania ,Pollution ,chemistry ,engineering ,Environmental science ,Fertilizer ,Water quality ,business ,Water Pollutants, Chemical ,Environmental Monitoring - Abstract
An analysis of private potable water well data was conducted for seven single family residential developments in southern Chester County, Pennsylvania. Background data were available for 165 wells within the communities when the wells were first drilled in the 1980s and early 1990s. Sampling of 75 wells within these same communities was performed in 2006 to determine whether conversion of the land to residential housing along with the use of conventional on-lot septic systems had resulted in elevated concentration of nitrate-nitrogen in the drinking water aquifer. The data indicate that prior land use influenced the occurrence of nitrate-nitrogen in the drinking water aquifer. The median nitrate-nitrogen concentration for the 165 wells in the background dataset was 2.9 mg/L. One hundred-seven of those wells were drilled on land previously used for active agricultural purposes. The median nitrate concentration in these wells was 3.8 mg/L. Of 48 wells drilled on forested land, the median nitrate concentration was 1.1 mg/L, approximately 3.5 times lower than those drilled on active agricultural land. The median nitrate concentration in the 2006 sampling dataset was 3.6 mg/L, an increase of 0.7 mg/L. The data indicate that conversion of the land has not resulted in contamination of the drinking water aquifer with respect to nitrate-nitrogen. Likewise, the data suggest that the conversion has not resulted in significant improvements to overall water quality.
- Published
- 2011
205. TNF-α antagonist use and risk of hospitalization for infection in a national cohort of veterans with rheumatoid arthritis
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Prabha Ranganathan, Jeffrey R. Curtis, Angelique Zeringue, Liron Caplan, Jay R. McDonald, Seth A. Eisen, and Michael A. Lane
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Male ,Risk ,medicine.medical_specialty ,Time Factors ,Infections ,Article ,Etanercept ,Arthritis, Rheumatoid ,Cohort Studies ,Immunocompromised Host ,Internal medicine ,Adalimumab ,Medicine ,Humans ,Risk factor ,Veterans Affairs ,Aged ,Retrospective Studies ,business.industry ,Tumor Necrosis Factor-alpha ,Incidence ,Retrospective cohort study ,General Medicine ,Middle Aged ,medicine.disease ,Infliximab ,humanities ,United States ,Surgery ,Hospitalization ,United States Department of Veterans Affairs ,Rheumatoid arthritis ,Antirheumatic Agents ,Female ,business ,medicine.drug ,Cohort study - Abstract
Medications used to treat rheumatoid arthritis (RA) may confer an increased risk of infection. We conducted a retrospective cohort study of veterans with RA followed in the United States Department of Veterans Affairs health care system from October 1998 through September 2005. Risk of hospitalization for infection associated with tumor necrosis factor (TNF)-α antagonists therapy was measured using an extension of Cox proportional hazards regression, adjusting for demographic characteristics, comorbid illnesses, and other medications used to treat RA. A total of 20,814 patients met inclusion criteria, including 3796 patients who received infliximab, etanercept, or adalimumab. Among the study cohort, 1465 patients (7.0%) were hospitalized at least once for infection. There were 1889 hospitalizations for infection. The most common hospitalized infections were pneumonia, bronchitis, and cellulitis. Age and several comorbid medical conditions were associated with hospitalization for infection. Prednisone (hazard ratio [HR], 2.14; 95% confidence interval [CI], 1.88-2.43) and TNF-α antagonist use (HR, 1.24; 95% CI, 1.02-1.50) were associated with hospitalization for infection, while the use of disease-modifying antirheumatic drugs (DMARDs) other than TNF-α antagonists was not. Compared to etanercept, infliximab was associated with risk for hospitalization for infection (HR, 1.51; 95% CI, 1.14-2.00), while adalimumab use was not (HR, 0.95; 95% CI, 0.68-1.33). In all treatment groups, rate of hospitalization for infection was highest in the first 8 months of therapy. We conclude that patients with RA who are treated with TNF-α antagonists are at higher risk for hospitalization for infection than those treated with other DMARDs. Prednisone use is also a risk factor for hospitalization for infection.
- Published
- 2011
206. Hypoglossal Neuropathology and Respiratory Activity in Pompe Mice
- Author
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Kai Qiu, Michael A. Lane, Milapjit S. Sandhu, Barry J. Byrne, Darin J. Falk, Mai K. ElMallah, Paul J. Reier, David D. Fuller, and Kun-Ze Lee
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Pathology ,medicine.medical_specialty ,Physiology ,Central nervous system ,Periodic acid–Schiff stain ,Neuropathology ,lcsh:Physiology ,brainstem ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Physiology (medical) ,Glycogen storage disease type II ,medicine ,hypoglossal ,Original Research ,030304 developmental biology ,0303 health sciences ,lcsh:QP1-981 ,Glycogen ,business.industry ,acid alpha-glucosidase ,medicine.disease ,3. Good health ,Hypoventilation ,medicine.anatomical_structure ,Pompe ,chemistry ,acid α-glucosidase ,Acid alpha-glucosidase ,Brainstem ,medicine.symptom ,business ,030217 neurology & neurosurgery - Abstract
Pompe disease is a lysosomal storage disorder associated with systemic deficiency of acid α-glucosidase (GAA). Respiratory-related problems in Pompe disease include hypoventilation and upper airway dysfunction. Although these problems have generally been attributed to muscular pathology, recent work has highlighted the potential role of central nervous system (CNS) neuropathology in Pompe motor deficiencies. We used a murine model of Pompe disease to test the hypothesis that systemic GAA deficiency is associated with hypoglossal (XII) motoneuron pathology and altered XII motor output during breathing. Brainstem tissue was harvested from adult Gaa-/- mice and the periodic acid Schiff (PAS) method was used to examine neuronal glycogen accumulation. Semi-thin (2 µm) plastic sections showed widespread medullary neuropathology with extensive cytoplasmic glycogen accumulation in XII motoneuron soma. We next recorded efferent XII bursting in anesthetized and ventilated Gaa-/- and B6/129 mice both before and after bilateral vagotomy. The coefficient of variation of respiratory cycle duration was greater in Gaa-/- compared to B6/129 mice (P < 0.01). Vagotomy caused a robust increase in XII inspiratory burst amplitude in B6/129 mice (239 ± 44 %baseline; P < 0.01) but had little impact on burst amplitude in Gaa-/- mice (130 ± 23 %baseline; P > 0.05). We conclude that CNS GAA deficiency results in substantial glycogen accumulation in XII motoneuron cell bodies and altered XII motor output. Therapeutic strategies targeting the CNS may be required to fully correct respiratory-related deficits in Pompe disease.
- Published
- 2011
207. Operations Research Techniques: A Longitudinal Update 1973–1988
- Author
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Ali H. Mansour, Michael S. Lane, and John L. Harpell
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Set (abstract data type) ,Operations research ,Computer science ,Management science ,Management of Technology and Innovation ,Strategy and Management ,Foundation (evidence) ,Management Science and Operations Research ,Math programming - Abstract
Questionnaires have been sent to Operations Research Society of America (ORSA) members at five-year intervals over the past 15 years (1973, 1978, 1983, 1988). The most recent set of questionnaires (1988) indicates what operations research (OR) educators and practitioners believe are the quantitative techniques needed for a proper foundation in OR. The results show some change since the first questionnaire (1973). Three quantitative techniques stand out as consistently believed to be the most important: math programming, statistics, and simulation. Other techniques vary in relative importance. Practitioners indicate the use of a more diverse set of techniques than educators.
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- 1993
208. Cryptosporidium parvum Infection Requires Host Cell Actin Polymerization
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Laura M. Machesky, Robin C. May, Douglas P. Clark, Michael A. Lane, David A. Elliott, and Daniel J. Coleman
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Polymers ,Immunology ,Cryptosporidiosis ,Wiskott-Aldrich Syndrome Protein, Neuronal ,Nerve Tissue Proteins ,macromolecular substances ,Biology ,Neural Wiskott-Aldrich Syndrome Protein ,Microbiology ,Cell Line ,Host-Parasite Interactions ,parasitic diseases ,Animals ,Humans ,Cytoskeleton ,Actin ,Cryptosporidium parvum ,Cellular Microbiology: Pathogen-Host Cell Molecular Interactions ,Microfilament Proteins ,Phosphoproteins ,biology.organism_classification ,Actins ,Cell biology ,Cytoskeletal Proteins ,Infectious Diseases ,Cytoplasm ,Actin-Related Protein 3 ,Actin-Related Protein 2 ,Host cell cytoplasm ,Parasitology ,Cell Adhesion Molecules ,Intracellular - Abstract
The intracellular protozoan parasite Cryptosporidium parvum accumulates host cell actin at the interface between the parasite and the host cell cytoplasm. Here we show that the actin polymerizing proteins Arp2/3, vasodilator-stimulated phosphoprotein (VASP), and neural Wiskott Aldrich syndrome protein (N-WASP) are present at this interface and that host cell actin polymerization is necessary for parasite infection.
- Published
- 2001
209. Prophylactic antibiotics in aesthetic surgery
- Author
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Bernard C. Camins, Michael A. Lane, and V. Leroy Young
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medicine.medical_specialty ,medicine.drug_class ,Antibiotics ,MEDLINE ,Antisepsis ,Hair Removal ,Patient safety ,Cost of Illness ,Hypothermia, Induced ,Medicine ,Infection control ,Humans ,Surgical Wound Infection ,Antibiotic prophylaxis ,Glycemic ,business.industry ,Smoking ,General Medicine ,Antibiotic Prophylaxis ,Plastic Surgery Procedures ,Drug Utilization ,Surgery ,Hyperglycemia ,business ,Skin preparation ,Medical literature - Abstract
Improvements in infection prevention practices over the past several decades have enhanced outcomes following aesthetic surgery. However, surgical site infections (SSI) continue to result in increased morbidity, mortality, and cost of care. The true incidence rate of SSI in aesthetic surgery is unknown due to the lack of a national surveillance system, but studies of SSI across surgical specialties have suggested that many of these infections are preventable. Patient-related factors-including obesity, glycemic control, and tobacco use-may contribute to the development of SSI following aesthetic surgery. In terms of SSI prevention, proper handwashing and surgical skin preparation are integral. Furthermore, the administration of prophylactic antibiotics has been shown to reduce SSI following many types of surgical procedures. Unfortunately, there are few large, randomized studies examining the role of prophylactic antibiotics in aesthetic surgery. The authors review the medical literature, discuss the risks of antibiotic overutilization, and detail nonpharmacological methods for reducing the risk of SSI.
- Published
- 2010
210. Technical Support Document: 50% Energy Savings for Small Office Buildings
- Author
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Michael D. Lane, Brian A. Thornton, Weimin Wang, Yunzhi Huang, and Bing Liu
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Transport engineering ,Energy conservation ,Engineering ,Architectural engineering ,Payback period ,business.industry ,HVAC ,ASHRAE 90.1 ,Variable air volume ,Building design ,business ,Weighted arithmetic mean ,Efficient energy use - Abstract
The Technical Support Document (TSD) for 50% energy savings in small office buildings documents the analysis and results for a recommended package of energy efficiency measures (EEMs) referred to as the advanced EEMs. These are changes to a building design that will reduce energy usage. The package of advanced EEMs achieves a minimum of 50% energy savings and a construction area weighted average energy savings of 56.6% over the ANSI/ASHRAE/IESNA Standard 90.1-2004 for 16 cities which represent the full range of climate zones in the United States. The 50% goal is for site energy usage reduction. The weighted average is based on data on the building area of construction in the various climate locations. Cost-effectiveness of the EEMs is determined showing an average simple payback of 6.7 years for all 16 climate locations. An alternative set of results is provided which includes a variable air volume HVAC system that achieves at least 50% energy savings in 7 of the 16 climate zones with a construction area weighted average savings of 48.5%. Other packages of EEMs may also achieve 50% energy savings; this report does not consider all alternatives but rather presents at least one way to reach the goal. Design teams using this TSD should follow an integrated design approach and utilize additional analysis to evaluate the specific conditions of a project.
- Published
- 2010
211. The HP0256 gene product is involved in motility and cell envelope architecture of Helicobacter pylori
- Author
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Paul W. O'Toole, Delphine L. Caly, Kieran A. Ryan, Charles W. Penn, Stanley A. Moore, Michael C. Lane, François P. Douillard, and Jason Hinds
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Microbiology (medical) ,FliJ ,Cell envelope architecture ,Mutant ,HP0256 ,lcsh:QR1-502 ,Flagellum ,Microbiology ,Bacterial Adhesion ,lcsh:Microbiology ,Cell Line ,Gene product ,03 medical and health sciences ,Bacterial Proteins ,Microscopy, Electron, Transmission ,Salmonella ,Research article ,Humans ,Adhesins, Bacterial ,Ulcer ,Oligonucleotide Array Sequence Analysis ,030304 developmental biology ,0303 health sciences ,biology ,Helicobacter pylori ,030306 microbiology ,Gene Expression Profiling ,Cell Membrane ,Computational Biology ,Epithelial Cells ,biology.organism_classification ,Transport protein ,Cell biology ,Regulon ,Flagella ,Gastritis ,biology.protein ,Bacterial outer membrane ,Gene Deletion ,Locomotion ,Flagellin - Abstract
Background Helicobacter pylori is the causative agent for gastritis, and peptic and duodenal ulcers. The bacterium displays 5-6 polar sheathed flagella that are essential for colonisation and persistence in the gastric mucosa. The biochemistry and genetics of flagellar biogenesis in H. pylori has not been fully elucidated. Bioinformatics analysis suggested that the gene HP0256, annotated as hypothetical, was a FliJ homologue. In Salmonella, FliJ is a chaperone escort protein for FlgN and FliT, two proteins that themselves display chaperone activity for components of the hook, the rod and the filament. Results Ablation of the HP0256 gene in H. pylori significantly reduced motility. However, flagellin and hook protein synthesis was not affected in the HP0256 mutant. Transmission electron transmission microscopy revealed that the HP0256 mutant cells displayed a normal flagellum configuration, suggesting that HP0256 was not essential for assembly and polar localisation of the flagella in the cell. Interestingly, whole genome microarrays of an HP0256 mutant revealed transcriptional changes in a number of genes associated with the flagellar regulon and the cell envelope, such as outer membrane proteins and adhesins. Consistent with the array data, lack of the HP0256 gene significantly reduced adhesion and the inflammatory response in host cells. Conclusions We conclude that HP0256 is not a functional counterpart of FliJ in H. pylori. However, it is required for full motility and it is involved, possibly indirectly, in expression of outer membrane proteins and adhesins involved in pathogenesis and adhesion.
- Published
- 2010
212. Neuronal progenitor transplantation and respiratory outcomes following upper cervical spinal cord injury in adult rats
- Author
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Barbara E. O'Steen, Michael A. Lane, Todd E. White, Milapjit S. Sandhu, David D. Fuller, and Paul J. Reier
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Nerve net ,Central nervous system ,Article ,Central nervous system disease ,Rats, Sprague-Dawley ,Developmental Neuroscience ,Medicine ,Animals ,Spinal cord injury ,Spinal Cord Injuries ,Phrenic nerve ,business.industry ,Stem Cells ,Graft Survival ,Age Factors ,Recovery of Function ,medicine.disease ,Spinal cord ,Respiratory Paralysis ,Rats ,Transplantation ,Electrophysiology ,Disease Models, Animal ,medicine.anatomical_structure ,Neurology ,nervous system ,Spinal Cord ,Tissue Transplantation ,Cervical Vertebrae ,Female ,Nerve Net ,business ,Neuroscience ,Microdissection ,Stem Cell Transplantation - Abstract
Despite extensive gray matter loss following spinal cord injury (SCI), little attention has been given to neuronal replacement strategies and their effects on specific functional circuits in the injured spinal cord. In the present study, we assessed breathing behavior and phrenic nerve electrophysiological activity following transplantation of microdissected dorsal or ventral pieces of rat fetal spinal cord tissue (FSC(D) or FSC(V), respectively) into acute, cervical (C2) spinal hemisections. Transneuronal tracing demonstrated connectivity between donor neurons from both sources and the host phrenic circuitry. Phrenic nerve recordings revealed differential effects of dorsally vs. ventrally derived neural progenitors on ipsilateral phrenic nerve recovery and activity. These initial results suggest that local gray matter repair can influence motoneuron function in targeted circuits following spinal cord injury and that outcomes will be dependent on the properties and phenotypic fates of the donor cells employed.
- Published
- 2010
213. Restorative and Compensatory Respiratory Plasticity Following Cervical Spinal Cord Injury (SCI)
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Krystal Salazar, David D. Fuller, Jennie C. Vavrousek, Lynne M. Mercier, Michael A. Lane, Barbara E. O'Steen, John W. Meyer, and Paul J. Reier
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business.industry ,Anesthesia ,Cervical spinal cord injury ,Genetics ,Medicine ,Respiratory system ,Plasticity ,business ,Molecular Biology ,Biochemistry ,Biotechnology - Published
- 2010
214. Book Review of The People of Knossos: Prosopographical Studies in the Knossos Linear B Archives, by Hedvig Landenius Enegren
- Author
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Michael Franklin Lane
- Subjects
Literature ,Archeology ,History ,business.industry ,Prosopography ,business ,Classics - Published
- 2010
215. Application of SONET Adaptive Rate to Multi-Carriers Transport Network
- Author
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Michael F. Lane and Nee Ben Gee
- Subjects
Engineering ,business.industry ,Synchronous optical networking ,Adaptive system ,Transport network ,Bandwidth (signal processing) ,Telecommunications service ,Service provider ,business ,Service provisioning ,Maintenance engineering ,Computer network - Abstract
This paper provides a current assessment of SONET Adaptive Rate technique from the local access service provider perspective. It also highlights how it shortens the service provisioning and activation processes from weeks to hours.
- Published
- 2010
216. Teaching business ethics: Bringing reality to the classroom
- Author
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Michael S. Lane and Dietrich L. Schaupp
- Subjects
Alternative methods ,Economics and Econometrics ,Ethical issues ,business.industry ,Public relations ,General Business, Management and Accounting ,Newspaper ,Arts and Humanities (miscellaneous) ,Sociology ,Business and International Management ,Business ethics ,business ,Law ,Quality of Life Research - Abstract
This paper presents an alternative method for discussing ethical issues. The method supports the use of the real world situations and emphasizes the interaction of all constituencies. The method incorporates the use of newspaper reports of real-life occurrences. It also stresses the use of local stories when possible.
- Published
- 1992
217. Variation in genomic alu repeat density as a basis for rapid construction of low resolution physical maps of human chromosomes
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Richar E. Kouri, Douglas J. Hanlon, Michael J. Lane, Peter J. Hahn, Clark S. Huckaby, Scott M. Peshick, Seth Mante, Brian D. Faldasz, John C. Hozier, William T. Carroll, Jane M. Scalzi, P. Greg Waterbury, and Anne M. Smardon
- Subjects
Genetics ,biology ,Chromosome Mapping ,Genetic Variation ,Nucleic Acid Hybridization ,Chromosome ,Alu element ,Hybrid Cells ,Restriction fragment ,Chromosome 17 (human) ,chemistry.chemical_compound ,chemistry ,Karyotyping ,biology.protein ,Humans ,Genomic library ,Human genome ,Molecular probe ,Genetics (clinical) ,DNA ,Chromosomes, Human, Pair 17 ,Repetitive Sequences, Nucleic Acid - Abstract
Human DNA restriction fragments containing high numbers of Alu repeat sequences can be preferentially detected in the presence of other human DNA restriction fragments in DNA from human: rodent somatic cell hybrids when the DNA is fragmented with enzymes that cleave mammalian DNA infrequently. This ability to lower the observed human DNA complexity allowed us to develop an approach to order rapidly somatic hybrid cell lines retaining overlapping human genomic domains. The ordering process also generates a relative physical map of the human fragments detected with Alu probe DNA. This process can generate physical mapping information for human genomic domains as large as an entire chromosome (100,000 kb). The strategy is demonstrated by ordering Alu-detected NotI fragments in a panel of mouse: human hybrid cells that span the entire long arm of human chromosome 17.
- Published
- 1992
218. Double-minute chromosomes as megabase cloning vehicles
- Author
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John A. Longo, Peter J. Hahn, Jane M. Scalzi, Michael J. Lane, Leanna Giddings, and John C. Hozier
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Genetic Vectors ,CHO Cells ,Hybrid Cells ,Biology ,Applied Microbiology and Biotechnology ,Chromosomes ,Mice ,chemistry.chemical_compound ,Plasmid ,Cricetinae ,Genetics ,Animals ,Double minute ,Cloning, Molecular ,Selection, Genetic ,Cells, Cultured ,Selectable marker ,Mammals ,Cloning ,Chinese hamster ovary cell ,Gene Amplification ,Chromosome ,Molecular biology ,Restriction enzyme ,chemistry ,Mutagenesis ,Chromosome Deletion ,DNA - Abstract
Radiation-reduced chromosomes provide valuable reagents for cloning and mapping genes, but they require multiple rounds of x-ray deletion mutagenesis to excise unwanted chromosomal DNA while maintaining physical attachment of the desired DNA to functional host centromere and telomere sequences. This requirement for chromosomal rearrangements can result in undesirable x-ray induced chromosome chimeras where multiple non-contiguous chromosomal fragments are fused. We have developed a cloning system for maintaining large donor subchromosomal fragments of mammalian DNA in the megabase size range as acentric chromosome fragments (double-minutes) in cultured mouse cells. This strategy relies on randomly inserted selectable markers for donor fragment maintenance. As a test case, we have cloned random segments of Chinese hamster ovary (CHO) chromosomal DNA in mouse EMT-6 cells. This was done by cotransfecting plasmids pZIPNeo and pSV2dhfr into DHFR-CHO cells followed by isolation of a Neo + DHFR + CHO donor colony and radiation-fusion-hybridization (RFH) to EMT-6 cells. We then selected for initial resistance to G418 and then to increasing levels of methotrexate (MTX). Southern analysis of pulsed-field gel electrophoresis of rare-cutting restriction endonuclease digestions of DNA from five RFH isolates indicated that all five contain at least 600 kb of unrearranged CHO DNA. In situ hybridization with the plasmids pZIPNeo and pSV2dhfr to metaphase chromosomes of MTX-resistant hybrid EMT-6 lines indicated that these markers reside on double-minute chromosomes.
- Published
- 1992
219. Gilvocarcin V exhibits both equilibrium DNA binding and UV light induced DNA adduct formation which is sequence context dependent
- Author
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R M Knobler, F B Radlwimmer, and Michael J. Lane
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Antibiotics, Antineoplastic ,Base Sequence ,Pyrimidine ,Ultraviolet Rays ,Stereochemistry ,Guanine ,Molecular Sequence Data ,Biology ,Anti-Bacterial Agents ,Thymine ,Adduct ,chemistry.chemical_compound ,Aminoglycosides ,Oligodeoxyribonucleotides ,chemistry ,Biochemistry ,Coumarins ,Mutagenesis ,Duplex (building) ,Genetics ,Ultraviolet light ,Deoxyribonuclease I ,Glycosides ,DNA - Abstract
The relative degree of both equilibrium binding and of ultraviolet light induced adduct formation for the antitumor antibiotic gilvocarin V with two hexaecamer DNA sequence isomers, d[ATATATAGCTATATAT]2 and d[AAAAAAAGCTTTTTTT]2, was assessed. The experiments reveal that gilvocarin V binds, under equilibrium conditions, and reacts, in the presence of exogenously applied UV light, more efficiently with the alternating purine:pyrimidine sequence hexadecamer than the homopurine:homopyrimidine duplex at identical gilvocarcin V to DNA duplex ratios. DNAse I digests of adduct containing duplexes derived from the d[AAAAAAAGCTTTTTTT]2 duplex, identified and isolated using gel shift assays employing denaturing polyacrylamide gels, confirm that gilvocarcin V adducts can be formed with thymine residues but suggest that adduct formation with either adenine or guanine residues is also possible.
- Published
- 1992
220. Technical Support Document: 50% Energy Savings Design Technology Packages for Highway Lodging Buildings
- Author
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Bing Liu, Brian A. Thornton, Michael I. Rosenberg, Michael D. Lane, Wei Jiang, and Krishnan Gowri
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Transport engineering ,Energy conservation ,Architectural engineering ,Technical support ,Engineering ,Process (engineering) ,business.industry ,ASHRAE 90.1 ,business ,Energy engineering ,Energy (signal processing) ,Efficient energy use ,Design technology - Abstract
This Technical Support Document (TSD) describes the process, methodology and assumptions for development of the 50% Energy Savings Design Technology Packages for Highway Lodging Buildings, a design guidance document intended to provide recommendations for achieving 50% energy savings in highway lodging properties over the energy-efficiency levels contained in ANSI/ASHRAE/IESNA Standard 90.1-2004, Energy Standard for Buildings Except Low-Rise Residential Buildings.
- Published
- 2009
221. Technical Support Document: 50% Energy Savings Design Technology Packages for Medium Office Buildings
- Author
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Brian A. Thornton, Michael D. Lane, Weimin Wang, Michael I. Rosenberg, and Bing Liu
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Technical support ,Engineering ,Architectural engineering ,Process (engineering) ,business.industry ,ASHRAE 90.1 ,business ,Design guide ,Energy (signal processing) ,Design technology - Abstract
This Technical Support Document (TSD) describes the process and methodology for development of the Advanced Energy Design Guide for Medium Offices (AEDG-MO or the Guide), a design guidance document which intends to provide recommendations for achieving 50% energy savings in medium office buildings that just meet the requirements of ANSI/ASHRAE/IESNA Standard 90.1-2004, Energy Standard for Buildings Except Low-Rise Residential Buildings.
- Published
- 2009
222. Spinal circuitry and respiratory recovery following spinal cord injury
- Author
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Kun-Ze Lee, David D. Fuller, Michael A. Lane, and Paul J. Reier
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Pulmonary and Respiratory Medicine ,Interneuron ,Physiology ,Neural substrate ,Central nervous system ,Spontaneous recovery ,Respiratory System ,Biology ,Article ,Interneurons ,Neuroplasticity ,medicine ,Animals ,Humans ,Spinal cord injury ,Spinal Cord Injuries ,Phrenic nerve ,Motor Neurons ,General Neuroscience ,Recovery of Function ,Spinal cord ,medicine.disease ,Phrenic Nerve ,medicine.anatomical_structure ,nervous system ,Spinal Cord ,Cervical Vertebrae ,Nerve Net ,Neuroscience - Abstract
Numerous studies have demonstrated anatomical and functional neuroplasticity following spinal cord injury. One of the more notable examples is return of ipsilateral phrenic motoneuron and diaphragm activity which can be induced under terminal neurophysiological conditions after high cervical hemisection in the rat. More recently it has been shown that a protracted, spontaneous recovery also occurs in this model. While a candidate neural substrate has been identified for the former, the neuroanatomical basis underlying spontaneous recovery has not been explored. Demonstrations of spinal respiratory interneurons in other species suggest such cells may play a role; however, the presence of interneurons in the adult rat phrenic circuit – the primary animal model of respiratory plasticity – has not been extensively investigated. Emerging neuroanatomical and electrophysiological results raise the possibility of a more complex neural network underlying spontaneous recovery of phrenic function and compensatory respiratory neuroplasticity after C2 hemisection than has been previously considered.
- Published
- 2009
223. Human paragonimiasis in North America following ingestion of raw crayfish
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Sam J. Lubner, Michael A. Lane, Michael Yeung, Mary C. Barsanti, Gary J. Weil, and Carlos A. Q. Santos
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Microbiology (medical) ,Adult ,Male ,medicine.medical_specialty ,Paragonimus westermani ,Pathology ,Hemoptysis ,Tuberculosis ,Paragonimiasis ,Lung Diseases, Parasitic ,Food Contamination ,Astacoidea ,Praziquantel ,Diagnosis, Differential ,Food Parasitology ,Paragonimus ,parasitic diseases ,Medicine ,Helminths ,Animals ,Humans ,Shellfish ,Anthelmintics ,Missouri ,biology ,business.industry ,Zoonosis ,Crayfish ,medicine.disease ,biology.organism_classification ,Dermatology ,Infectious Diseases ,Paragonimus kellicotti ,Female ,business - Abstract
Paragonimiasis (human infections with the lung fluke Paragonimus westermani) is an important public health problem in parts of Southeast Asia and China. Paragonamiasis has rarely been reported from North America as a zoonosis caused by Paragonimus kellicotti. Paragonimus species have complex life cycles that require 2 intermediate hosts, namely, snails and crustaceans (ie, crabs or crayfish). Humans acquire P. kellicotti when they consume infected raw crayfish. Humans with paragonimiasis usually present with fever and cough, which, together with the presentation of hemoptysis, can be misdiagnosed as tuberculosis. Only 7 autochthonous cases of paragonimiasis have been previously reported from North America. Our study describes 3 patients with proven or probable paragonimiasis with unusual clinical features who were seen at a single medical center during an 18-month period. These patients acquired their infections after consuming raw crayfish from rivers in Missouri. It is likely that other patients with paragonimiasis have been misdiagnosed and improperly treated. Physicians should consider the possibility that patients who present with cough, fever, hemoptysis, and eosinophilia may have paragonimiasis.
- Published
- 2009
224. Pre‐phrenic interneurons as an anatomical substrate for plasticity following cervical spinal cord injury (SCI) in the adult rat
- Author
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Forest L. Hunsaker, Jennie C. Vavrousek, Barbara E. O'Steen, Krystal Salazar, David D. Fuller, Michael A. Lane, Paul J. Reier, and Alex L. Jones
- Subjects
business.industry ,Cervical spinal cord injury ,Genetics ,Substrate (chemistry) ,Medicine ,Anatomy ,Plasticity ,business ,Molecular Biology ,Biochemistry ,Biotechnology - Published
- 2009
225. Spinal Cord: Repair and Rehabilitation
- Author
-
Michael A. Lane, Andrea L Behrman, Paul J. Reier, and Dena R. Howland
- Subjects
medicine.medical_specialty ,Rehabilitation ,Repair processes ,business.industry ,medicine.medical_treatment ,Spinal cord ,medicine.disease ,Spinal cord repair ,Locomotor training ,medicine.anatomical_structure ,Physical medicine and rehabilitation ,Neuroplasticity ,medicine ,business ,Neuroscience ,Spinal cord injury ,Spinal Cord Regeneration - Abstract
Functional deficits following spinal cord injury are no longer viewed as totally irreversible. Laboratory findings indicate the ability to walk can be partially restored by individual or combined therapeutic approaches targeting specific cellular responses to trauma. In addition, certain rehabilitation strategies can enhance ambulatory capacity, as well as stimulate certain intrinsic cellular or molecular repair processes. Other findings indicate the spinal cord is not as ‘hard-wired’ as previously believed, and that neural circuitries in the injured spinal cord undergo natural remodelling with some changes appearing to facilitate some improvements in function over time. These collective observations highlight intriguing opportunities for achieving better functional outcomes and quality of life by interfacing cellular and molecular treatments with rehabilitation techniques that can amplify natural or therapeutically directed repair mechanisms. Whereas challenges remain, interactive training/exercise is becoming recognized as a fundamental adjunct to a growing list of potential therapeutic interventions for promoting spinal cord regeneration and neuroplasticity. Keywords: neuroplasticity; locomotor training; rehabilitation; spinal cord injury; spinal cord repair
- Published
- 2009
226. Remotely Switched OTDR Conformance Testing at the Fiber Distribution Hub
- Author
-
John G. Berger and Michael F. Lane
- Subjects
Engineering ,Optical fiber ,business.industry ,media_common.quotation_subject ,Fiber (computer science) ,Optical time-domain reflectometer ,Passive optical network ,Optical switch ,law.invention ,law ,Test set ,Electronic engineering ,Quality (business) ,business ,Conformance testing ,Computer hardware ,media_common - Abstract
Conformance testing is a critical step in ensuring the future quality of a passive optical network. A new automated test procedure couples an optical switch and a hand-held test set to enable significant labor savings.
- Published
- 2009
227. Repair of Dielectric Interfaces with Chemistry Specific Coupling Agents
- Author
-
Michael W. Lane, Abigail Roush, Stephen E. Callahan, Paul S. Ho, Ehrenfried Zschech, and Shinichi Ogawa
- Subjects
Reliability (semiconductor) ,Materials science ,Coupling (computer programming) ,business.industry ,Interface (computing) ,Delamination ,Forensic engineering ,Microelectronics ,Mechanical engineering ,Node (circuits) ,Dielectric ,business ,Electromigration - Abstract
Low dielectric constant (low‐k) materials are currently being incorporated into advanced microelectronic devices to improve or maintain performance. As the dielectric constant is reduced, so are its mechanical properties. These reduced properties have recently been related to chip‐package interaction (CPI) failures. Significant effort has focused on eliminating CPI failures through engineering of copper crackstop structures. However, published data suggests that crackstop engineering needs to occur at each technology node to ensure CPI reliability. In this study, the focus is on repairing interfacial delaminations with chemistry specific coupling agents rather than attempting to stop them with a specially designed crackstop structure. Critical adhesion values and corrosion resistance of the repaired interfaces are compared to the original interface. The application of the repair chemistry in an integrated structure is discussed along with the potential impact on reliability.
- Published
- 2009
228. An international study of ethics
- Author
-
Michael S. Lane, Dietrich L. Schaupp, and Hans Pohl
- Subjects
German ,Philosophy ,Medical education ,Graduate students ,ComputingMilieux_COMPUTERSANDEDUCATION ,language ,Sociology ,American business ,General Business, Management and Accounting ,language.human_language ,Management - Abstract
This study presents the results of a survey of graduate students from three cultural backgrounds. English, German, and American business students responded to an eleven-item survey designed to assess one's beliefs about what is necessary to succeed in graduate business programs. The results indicate that the attributes which students believe are required to succeed differ among cultures, with the greatest differences occurring between the American and European respondents.
- Published
- 1991
229. Actinomycin D induced DNase I hypersensitivity and asymmetric structure transmission in a DNA hexadecamer
- Author
-
Michael J. Lane, Philip N. Borer, Yao qi Huang, and Karl D. Bishop
- Subjects
Conformational change ,Magnetic Resonance Spectroscopy ,Base Sequence ,Oligonucleotide ,Molecular Sequence Data ,Nucleic Acid Heteroduplexes ,Nuclear magnetic resonance spectroscopy ,Biology ,Cleavage (embryo) ,Molecular biology ,Intercalating Agents ,chemistry.chemical_compound ,chemistry ,Dactinomycin ,Genetics ,Biophysics ,Drug Binding Site ,Deoxyribonuclease I ,Nucleic Acid Conformation ,A-DNA ,sense organs ,skin and connective tissue diseases ,DNA - Abstract
DNase I cleavage rates and nmr chemical shifts are shown to change for DNA sequences distal to an intercalated actinomycin D molecule in a duplex hexadecamer upon drug binding. Both sets of observations suggest that the source of these changes is a DNA-mediated structural response. The nmr results imply the response is transmitted preferentially in a 5'-to-3' direction from the drug binding site. An inequivalent response of the two strands to a ligand-induced conformational change immediately suggests a mechanism for distinguishing the sense and antisense strands of DNA.
- Published
- 1991
230. Cervical Pre-Phrenic Interneurons in the Normal and Lesioned Spinal Cord of the Adult Rat
- Author
-
Donald C. Bolser, Bill J. Yates, Todd E. White, Marcella A. Coutts, Milapjit S. Sandhu, David C. Bloom, David D. Fuller, Michael A. Lane, Alex L. Jones, and Paul J. Reier
- Subjects
Nerve net ,Diaphragm ,Biology ,Article ,Functional Laterality ,Rats, Sprague-Dawley ,Interneurons ,Neural Pathways ,medicine ,Animals ,Spinal cord injury ,Spinal Cord Injuries ,Phrenic nerve ,Motor Neurons ,Brain Mapping ,Medulla Oblongata ,Staining and Labeling ,General Neuroscience ,musculoskeletal, neural, and ocular physiology ,Respiratory center ,Anatomy ,Recovery of Function ,Respiratory Center ,Spinal cord ,medicine.disease ,musculoskeletal system ,Retrograde tracing ,Herpesvirus 1, Suid ,Electric Stimulation ,Rats ,Phrenic Nerve ,Anterograde tracing ,medicine.anatomical_structure ,nervous system ,Spinal Cord ,Medulla oblongata ,Respiratory Physiological Phenomena ,Female ,Nerve Net ,Neuroscience ,Biomarkers ,circulatory and respiratory physiology - Abstract
Although monosynaptic bulbospinal projections to phrenic motoneurons have been extensively described, little is known about the organization of phrenic premotor neurons in the adult rat spinal cord. Because interneurons may play an important role in normal breathing and recovery following spinal cord injury, the present study has used anterograde and transneuronal retrograde tracing to study their distribution and synaptic relations. Exclusive unilateral, first-order labeling of the phrenic motoneuron pool with pseudorabies virus demonstrated a substantial number of second-order, bilaterally distributed cervical interneurons predominantly in the dorsal horn and around the central canal. Combined transneuronal and anterograde tracing revealed ventral respiratory column projections to prephrenic interneurons, suggesting that some propriospinal relays exist between medullary neurons and the phrenic nucleus. Dual-labeling studies with pseudorabies virus recombinants also showed prephrenic interneurons integrated with either contralateral phrenic or intercostal motoneuron pools. The stability of interneuronal pseudorabies virus labeling patterns following lateral cervical hemisection was then addressed. Except for fewer infected contralateral interneurons at the level of the central canal, the number and distribution of phrenic-associated interneurons was not significantly altered 2 weeks posthemisection (i.e., the point at which the earliest postinjury recovery of phrenic activity has been reported). These results demonstrate a heterogeneous population of phrenic-related interneurons. Their connectivity and relative stability after cervical hemisection raise speculation for potentially diverse roles in modulating phrenic function normally and postinjury.
- Published
- 2008
231. Respiratory neuroplasticity and cervical spinal cord injury: translational perspectives
- Author
-
Michael A. Lane, David D. Fuller, Todd E. White, and Paul J. Reier
- Subjects
Neuronal Plasticity ,General Neuroscience ,Spontaneous recovery ,Respiratory System ,Neurological disorder ,Motor neuron ,medicine.disease ,musculoskeletal system ,Article ,Diaphragm (structural system) ,Rats ,Lesion ,Disease Models, Animal ,medicine.anatomical_structure ,nervous system ,Neuroplasticity ,medicine ,Breathing ,Paralysis ,Animals ,Humans ,medicine.symptom ,Psychology ,Neuroscience ,Spinal Cord Injuries - Abstract
Paralysis of the diaphragm is a severe consequence of cervical spinal cord injury. This condition can be experimentally modeled by lateralized, high cervical lesions that interrupt descending inspiratory drive to the corresponding phrenic nucleus. Although partial recovery of ipsilateral diaphragm function occurs over time, recent findings show persisting chronic deficits in ventilation and phrenic motoneuron activity. Some evidence suggests, however, that spontaneous recovery can be enhanced by modulating neural pathways to phrenic motoneurons via synaptic circuitries which appear more complex than previously envisioned. The present review highlights these and other recent experimental multi-disciplinary findings pertaining to respiratory neuroplasticity in the rat. Translational considerations are also emphasized, with specific attention directed at the clinical and interpretational strengths of different lesion models and outcome measures.
- Published
- 2008
232. First Trial of Maintenance Friendly Network (MFN) by Switching Spare Field Fibers without Traffic Interruption
- Author
-
Avigdor Shlomovits, Hubert Beuerlein, Michael F. Lane, Glenn A. Wellbrock, Michael D. Pollock, Sandy Roskes, Joe Teixeira, T. J. Xia, and Ze'ev Ganor
- Subjects
Engineering ,business.industry ,Synchronous optical networking ,Spare part ,Electronic engineering ,Primary School Evaluation Test ,business ,Optical switch ,Field (computer science) ,Computer network - Abstract
Maintenance friendly network is demonstrated for first time with three SONET protection schemes fed into a ULH system. Traffic can be switched between six field fibers without triggering protection on 1+1, UPSR, or BLSR configurations.
- Published
- 2008
233. Costs per Home Connected: The Impacts of Automated Fiber Management On Fiber-to-the-Home Deployments
- Author
-
Michael F. Lane, Nee-Ben Gee, Avigdor Shlomovits, Glenn A. Wellbrock, Joe Teixeira, T. J. Xia, Ze'ev Ganor, Joseph Finn, William Uliasz, and Sandy Roskes
- Subjects
Engineering ,business.industry ,Home automation ,Fiber (computer science) ,Capital cost ,Provisioning ,Economic impact analysis ,Troubleshooting ,Telecommunications ,business ,Activity-based costing ,Fiber to the x - Abstract
We demonstrate the economic impact of automated fiber management (AFM) in FTTH networks, specifically to defer capital costs and reduce operating costs by automating testing, provisioning, maintenance, troubleshooting, and grooming operations.
- Published
- 2008
234. Assessment and significance of phytoplankton species composition within Chesapeake Bay and Virginia tributaries through a long-term monitoring program
- Author
-
Michael F. Lane, Lubomira Burchardt, Kneeland K. Nesius, and Harold G. Marshall
- Subjects
Management, Monitoring, Policy and Law ,Rivers ,Phytoplankton ,Tributary ,Water Movements ,Dominance (ecology) ,Animals ,Humans ,Ecosystem ,Seawater ,Biomass ,General Environmental Science ,geography ,geography.geographical_feature_category ,biology ,Ecology ,Virginia ,Estuary ,General Medicine ,Plankton ,biology.organism_classification ,Pollution ,Monitoring program ,Diatom ,Environmental science ,Water Microbiology ,Environmental Monitoring - Abstract
Phytoplankton and water quality long term trends are presented from a 20-year monitoring program of Chesapeake Bay and several of its major tributaries. Increasing phytoplankton biomass and abundance are ongoing within this estuarine complex, with diatoms the dominant component, along with chlorophytes and cyanobacteria as sub-dominant contributors in the tidal freshwater and oligohaline regions. Diatoms, dinoflagellates, and cryptomonads are among the major flora downstream in the tributaries and within the Chesapeake Bay. Water quality conditions within the three tributaries have remained rather stable over this time period; while there are long term trends of reduced nutrients, increasing bottom oxygen, and decreasing water clarity for the lower Chesapeake Bay. Of note is an increasing trend of cyanobacteria biomass at 12 of the 13 stations monitored at tributary and Chesapeake Bay stations, plus the presence of 37 potentially harmful taxa reported for these waters. However, the overall status of the phytoplankton populations is presently favorable, in that it is mainly represented and dominated by taxa suitable as a major food and oxygen source within this ecosystem. Although potentially harmful taxa are present, they have not at this time exerted profound impact to the region, or replaced the diatom populations in overall dominance.
- Published
- 2008
235. Degeneration, Regeneration, and Plasticity in the Nervous System
- Author
-
Paul J. Reier and Michael A. Lane
- Published
- 2008
236. Computer Operations in Jordan
- Author
-
Michael S. Lane, Jack A. Fuller, and Ali H. Mansour
- Subjects
Data processing ,System development ,Engineering ,Process management ,Operations research ,business.industry ,Strategy and Management ,Business system planning ,Library and Information Sciences ,Business and International Management ,business ,Phase (combat) - Abstract
A computer survey of 101 data processing managers was conducted in Jordan to explore the life cycle of computer systems; sixty-two responses were collected. Six hypotheses were tested by chi-square analysis, two in each of the following systems phases: planning, acquisition, and operation. Four of the six hypotheses were supported. The two hypotheses dealing with the operation phase were not supported. The results of the study provided revealing insight into Jordan's progress and problems in the areas of system planning, acquisition, and operation. Specifically, the results indicate the importance of feasibility studies and managerial involvement in system planning, acquisition, and operation. In addition, the results indicate the importance of a strong ongoing relationship with local computer company representatives to ensure timely system updates.
- Published
- 1990
237. Emerging Network Need for Alien Wavelength Management
- Author
-
Michael F. Lane and David Z. Chen
- Subjects
Multiwavelength optical networking ,Engineering ,ComputingMilieux_THECOMPUTINGPROFESSION ,business.industry ,Optical cross-connect ,Interoperability ,Data_CODINGANDINFORMATIONTHEORY ,Optical performance monitoring ,Telecommunications network ,Passive optical network ,GeneralLiterature_MISCELLANEOUS ,Optical Transport Network ,10G-PON ,Telecommunications ,business - Abstract
Optical-transmission-distance reaches coast-to-coast without regeneration; costs have been reduced due to elimination of regenerations. Mixed-traffic-pattern leads to challenging task of managing the dynamic-wavelength which traverses multiple-network-domains. Effective optical interoperability starts with proper management of alien wavelength.
- Published
- 2007
238. Field Trial of Photonic Switches for Efficient Fiber Network Operation and Maintenance
- Author
-
Tiejun J. Xia, Michael F. Lane, Timothy E. Lawter, Glenn A. Wellbrock, Richard Jensen, Mike Bitting, Aaron Bent, Kevin Karch, Jonathan Lacey, and David Altstaetter
- Subjects
Microelectromechanical systems ,Computer science ,business.industry ,Field trial ,Electronic engineering ,Fiber network ,Photonics ,business - Abstract
Results of Verizon field trial using MEMS and beam-steering photonic switching technologies for automating network operation and maintenance functions show that operational expenses and downtime can be significantly reduced.
- Published
- 2007
239. Managing Fiber Connections in NGN and Applications
- Author
-
D. Kokkinos, David Z. Chen, and Michael F. Lane
- Subjects
Optical fiber cable ,business.industry ,Computer science ,Fiber (computer science) ,Optical performance monitoring ,Fiber to the x ,Networking hardware ,law.invention ,law ,Wavelength-division multiplexing ,Next-generation network ,Fiber ,Telecommunications ,business ,Computer network - Abstract
Increasing fiber applications such as DWDM, Ultra Long Haul and FTTH are rapidly taxing manually managed fiber infrastructure. New fiber management technologies and architectures are evaluated to meet the growth of emerging networks and applications.
- Published
- 2007
240. Use of Remote Software Download to Improve Operation Efficiency of Next -Generation Optical Transport Networks (NG-OTN)
- Author
-
Nee-Ben Gee, P.E. Phillips, Lily F. Chen, B. Basch, Vishnu Shukla, and Michael F. Lane
- Subjects
Engineering ,Network element ,Upgrade ,Optical Transport Network ,business.industry ,Backup ,Embedded system ,Synchronous optical networking ,Next-generation network ,Software performance testing ,business ,Networking hardware - Abstract
This paper presents the use of remote software download, upgrade, memory backup and restoration processes to improve operational efficiencies of NG-OTN. Network Element requirements, test results in a multi-vendor environment, and evolutionary considerations are presented.
- Published
- 2007
241. Myosin Heavy Chain Composition is Related to Vertical Jump Performance
- Author
-
Michael T. Lane, Andrew C. Fry, Trent J. Herda, Joseph P. Weir, and Ashley W. Herda
- Subjects
Vertical jump ,Chemical physics ,Chemistry ,Myosin ,Physical Therapy, Sports Therapy and Rehabilitation ,Orthopedics and Sports Medicine ,Composition (combinatorics) - Published
- 2015
242. Lipomas of the pancreas
- Author
-
K R Math, Michael J. Lane, Peter M. Nardi, Douglas S. Katz, A A Fruauff, R Barckhausen, and John Hines
- Subjects
Male ,medicine.medical_specialty ,Pancreatic disease ,Conservative management ,Radiologic sign ,X ray computed ,otorhinolaryngologic diseases ,medicine ,Humans ,Radiology, Nuclear Medicine and imaging ,Ct findings ,Aged ,business.industry ,General Medicine ,Middle Aged ,Lipoma ,medicine.disease ,Pancreatic Neoplasms ,body regions ,stomatognathic diseases ,medicine.anatomical_structure ,Radiology ,Tomography, X-Ray Computed ,business ,Pancreas - Abstract
OBJECTIVE: The purpose of this study was to describe the CT findings of pancreatic lipomas in four patients. CONCLUSION: In all four cases, the lipoma was revealed incidentally on CT scans obtained for other reasons. CT scans were diagnostic, showing well-circumscribed masses within the pancreas composed almost entirely of fat, with a few scattered vessels or septa or both, which ranged in size from 1.4 x 2.0 cm to 4.5 x 5.3 cm in the axial plane. Pancreatic lipomas are rare, usually incidental tumors and, as with lipomas found elsewhere in the body, conservative management is often indicated.
- Published
- 1998
243. Spontaneous intramural small bowel haemorrhage: Importance of non-contrast CT
- Author
-
R B Jeffrey, Douglas S. Katz, Robert E. Mindelzun, and Michael J. Lane
- Subjects
Adult ,Male ,medicine.medical_specialty ,Diagnostic information ,Non contrast ct ,Abdominal ct ,medicine ,Coagulopathy ,Humans ,Radiology, Nuclear Medicine and imaging ,In patient ,Duodenal Diseases ,Aged ,Retrospective Studies ,Hematoma ,business.industry ,Vascular disease ,Retrospective cohort study ,Jejunal Diseases ,General Medicine ,Blood Coagulation Disorders ,Middle Aged ,medicine.disease ,Surgery ,medicine.anatomical_structure ,Abdomen ,Female ,Radiology ,Gastrointestinal Hemorrhage ,Tomography, X-Ray Computed ,business - Abstract
The purpose of this study was to evaluate the abdominal CT findings in patients with spontaneous intramural small bowel haemorrhage. We retrospectively reviewed the abdominal CT scans of six patients with known intramural small bowel haemorrhage. All of the patients had an underlying coagulopathy. All six patients underwent CT examinations without oral or intravenous contrast media. All six non-contrast CT scans showed hyperattenuation of the involved bowel segments, with thickened and dilated proximal small bowel. Therefore, patients who are clinically at risk for intramural small bowel haemorrhage should undergo a non-contrast CT scan of the abdomen prior to the routine oral and intravenous contrast-enhanced scan. In most cases the non-contrast scan will provide definitive diagnostic information which may not be evident from the contrast-enhanced scan alone.
- Published
- 1997
244. Production, purification, crystallization and preliminary X-ray analysis of adeno-associated virus serotype 8
- Author
-
Nicholas Muzyczka, Barry J. Byrne, Eric Padron, Robert McKenna, Hyun Joo Nam, George Aslanidi, Michael Douglas Lane, Brittney Gurda-Whitaker, Mavis Agbandje-McKenna, Sergei Zolotukhin, and Eric Kohlbrenner
- Subjects
Insecta ,Viral protein ,viruses ,Biophysics ,Biology ,medicine.disease_cause ,Biochemistry ,law.invention ,Cell Line ,Viral Proteins ,Capsid ,X-Ray Diffraction ,Structural Biology ,law ,Genetics ,medicine ,Animals ,Crystallization ,Cloning, Molecular ,Adeno-associated virus ,Parvovirus ,Resolution (electron density) ,Space group ,Dependovirus ,Condensed Matter Physics ,biology.organism_classification ,Crystallography ,Crystallization Communications ,X-ray crystallography ,Capsid Proteins - Abstract
Adeno-associated viruses (AAVs) are actively being developed for clinical gene-therapy applications and the efficiencies of the vectors could be significantly improved by a detailed understanding of their viral capsid structures and the structural determinants of their tissue-transduction interactions. AAV8 is approximately 80% identical to the more widely studied AAV2, but its liver-transduction efficiency is significantly greater than that of AAV2 and other serotypes. The production, purification, crystallization and preliminary X-ray crystallographic analysis of AAV8 viral capsids are reported. The crystals diffract X-rays to 3.0 A resolution using synchrotron radiation and belong to the hexagonal space group P6(3)22, with unit-cell parameters a = 257.5, c = 443.5 A. The unit cell contains two viral particles, with ten capsid viral protein monomers per crystallographic asymmetric unit.
- Published
- 2005
245. Breakdown of the blood-brain barrier to proteins in white matter of the developing brain following systemic inflammation
- Author
-
Katarzyna M. Dziegielewska, A Potter, Mark D. Habgood, Norman R. Saunders, Helen B. Stolp, C J Ek, and Michael A. Lane
- Subjects
Lipopolysaccharides ,Pathology ,medicine.medical_specialty ,Histology ,medicine.medical_treatment ,Intraperitoneal injection ,Inflammation ,Brain damage ,Grey matter ,Biology ,Blood–brain barrier ,Systemic inflammation ,Permeability ,Pathology and Forensic Medicine ,White matter ,Rats, Sprague-Dawley ,Species Specificity ,medicine ,Animals ,Brain ,Cell Biology ,Blood Proteins ,Blood proteins ,Rats ,Monodelphis ,medicine.anatomical_structure ,Animals, Newborn ,Blood-Brain Barrier ,medicine.symptom - Abstract
Compromised blood-brain barrier permeability resulting from systemic inflammation has been implicated as a possible cause of brain damage in fetuses and newborns and may underlie white matter damage later in life. Rats at postnatal day (P) 0, P8 and P20 and opossums (Monodelphis domestica) at P15, P20, P35, P50 and P60 and adults of both species were injected intraperitoneally with 0.2-10 mg/kg body weight of 055:B5 lipopolysaccharide. An acute-phase response occurred in all animals. A change in the permeability of the blood-brain barrier to plasma proteins during a restricted period of postnatal development in both species was determined immunocytochemically by the presence of proteins surrounding cerebral blood vessels and in brain parenchyma. Blood vessels in white matter, but not grey matter, became transiently permeable to proteins between 10 and 24 h after lipopolysaccharide injection in P0 and P8 rats and P35-P60 opossums. Brains of Monodelphis younger than P35, rats older than P20 and adults of both species were not affected. Permeability of the blood-cerebrospinal fluid (CSF) barrier to proteins was not affected by systemic inflammation for at least 48 h after intraperitoneal injection of lipopolysaccharide. These results show that there is a restricted period in brain development when the blood-brain barrier, but not the blood-CSF barrier, to proteins is susceptible to systemic inflammation; this does not appear to be attributable to barrier "immaturity" but to its stage of development and only occurs in white matter.
- Published
- 2004
246. Marks, George Croydon, Baron Marks (1858–1938), engineer, patent agent, and politician
- Author
-
Michael R. Lane
- Published
- 2004
247. Rendel, James Meadows (1799–1856), civil engineer
- Author
-
Michael R. Lane
- Published
- 2004
248. Superinfection following smallpox vaccination (Vaccinia), United States: surveillance January 2003 through January 2004
- Author
-
Xiaojun Wen, Andrew Kroger, Francisco Averhoff, La Mar Hasbrouck, Allison Kennedy, Michael J. Lane, Claudia Vellozzi, Anne C. Moore, Christine G. Casey, and Scott Santibanez
- Subjects
Microbiology (medical) ,Adult ,Male ,Pediatrics ,medicine.medical_specialty ,viruses ,medicine.disease_cause ,complex mixtures ,chemistry.chemical_compound ,parasitic diseases ,medicine ,Vaccinia ,Smallpox ,Humans ,Overdiagnosis ,Smallpox vaccine ,Antibacterial agent ,Retrospective Studies ,business.industry ,virus diseases ,biochemical phenomena, metabolism, and nutrition ,Middle Aged ,medicine.disease ,Virology ,United States ,Vaccination ,Infectious Diseases ,chemistry ,Superinfection ,Population Surveillance ,Female ,Viral disease ,business - Abstract
BACKGROUND Superinfection is an adverse event following smallpox vaccination. The clinical presentation is similar to that of a large normal vaccine reaction or "robust take," and the frequency is unknown. METHODS We retrospectively reviewed all reported severe local reactions consistent with superinfection among United States civilian smallpox vaccinees from January 2003 through January 2004. We applied a standard case definition and estimated the frequency of superinfection following smallpox vaccination. RESULTS We identified 48 reported cases for further review among 39,350 [corrected] smallpox vaccinees. Two (4%) of the 48 reported cases met the case definition for superinfection; neither of the patients had a pathogenic organism isolated from their infection site. Both were treated with antibiotics and resolved their infection. Of the 46 cases determined not to be superinfection, 41 (89%) were temporally consistent with a large normal vaccine reaction. Thirty (75%) of 40 reported case patients for whom data were available received antibiotic therapy. CONCLUSIONS Superinfection following smallpox vaccination is rare. Most of the reported superinfection cases were probably large normal smallpox vaccine reactions. Educating providers about the normal response to smallpox vaccine may decrease the overdiagnosis of superinfection and the unnecessary use of antimicrobials.
- Published
- 2004
249. Regeneration of supraspinal axons after complete transection of the thoracic spinal cord in neonatal opossums (Monodelphis domestica)
- Author
-
Katarzyna M. Dziegielewska, Helen B. Stolp, Michael A. Lane, Norman R. Saunders, and Elizabeth Jane Fry
- Subjects
Programmed cell death ,Central nervous system ,Monodelphis domestica ,Thoracic Vertebrae ,Lumbar ,Neural Pathways ,medicine ,Animals ,Spinal cord injury ,Spinal Cord Injuries ,biology ,General Neuroscience ,Regeneration (biology) ,Age Factors ,Anatomy ,Opossums ,Spinal cord ,biology.organism_classification ,medicine.disease ,Axons ,Nerve Regeneration ,medicine.anatomical_structure ,nervous system ,Animals, Newborn ,Brainstem ,Brain Stem - Abstract
These studies define the time table and origin of supraspinal axons regenerating across a complete spinal transection in postnatal Monodelphis domestica. After lumbar (L1) spinal cord injection of fluorophore–dextran amine conjugate on postnatal (P) day 4, a consistent number of neurons could be labeled. The numbers of labeled neurons remained stable for several weeks, but subsequently declined by P60 in control animals and by P35 in animals with complete spinal transection (T4–T6) performed at P7. In control animals, 25–40% of neurons labeled with a fluorophore injected (L1) at P4 could also be double-labeled by a second fluorophore injected (T8–T10) at different older ages. In spinally transected animals, total numbers of neurons labeled with the second marker were initially lower compared with age-matched controls, but were not significantly different by 3 weeks after injury. The proportion of double-labeled neurons in spinally transected animals increased from approximately 2% 1 week after injury (P14) to approximately 50% by P60, indicating that a substantial proportion of neurons with axons transected at P7 is able to regenerate and persist into adulthood. However, the proportion of axons originating from regenerating neurons made only a small contribution at older ages to total numbers of fibers growing through the injury site, because much of development of the spinal cord occurs after P7. Evidence was obtained that degenerating neurons with both apoptotic and necrotic morphologies were present in brainstem nuclei; the number of neurons with necrotic morphology was much greater in the brainstem of animals with spinal cords transected at P7. J. Comp. Neurol. 466:422–444, 2003. © 2003 Wiley-Liss, Inc.
- Published
- 2003
250. Long-term phytoplankton trends and related water quality trends in the lower Chesapeake Bay, Virginia, USA
- Author
-
Harold G, Marshall, Michael F, Lane, and Kneeland K, Nesius
- Subjects
Maryland ,Nitrogen ,Phytoplankton ,Population Dynamics ,Virginia ,Water Pollutants ,Ecosystem ,Environmental Monitoring - Abstract
Long-term trends (i.e., 1985 through 1999; 14 1/2 yrs) of the phytoplankton community in Chesapeake Bay indicated patterns of increasing phytoplankton abundance and biomass associated with mainly diatoms and chlorophytes, and to a lesser degree dinoflagellates. Decreasing trends in productivity rates above the pycnocline were present over a shorter time period (10 1/2 yrs.), with evidence for increasing nitrogen limitation is indicated. Reduced light availability is inferred due to decreasing trends of Secchi depths and increased suspended solids trends, which were associated with decreasing trends in productivity rates.
- Published
- 2003
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