Search

Abstract Background The potential effect modification of sleep on the relationship between anxiety and elevated blood pressure (BP) in pregnancy is understudied. We evaluated the relationship between anxiety, insomnia, and short sleep duration, as well as any interaction effects between these variables, on BP during pregnancy. Methods This was a prospective pilot cohort of pregnant people between 23 to 36 weeks’ gestation at a single institution between 2021 and 2022. Standardized questionnaires were used to measure clinical insomnia and anxiety. Objective sleep duration was measured using a wrist-worn actigraphy device. Primary outcomes were systolic (SBP), diastolic (DBP), and mean (MAP) non-invasive BP measurements. Separate sequential multivariable linear regression models fit with generalized estimating equations (GEE) were used to separately assess associations between anxiety (independent variable) and each BP parameter (dependent variables), after adjusting for potential confounders (Model 1). Additional analyses were conducted adding insomnia and the interaction between anxiety and insomnia as independent variables (Model 2), and adding short sleep duration and the interaction between anxiety and short sleep duration as independent variables (Model 3), to evaluate any moderating effects on BP parameters. Results Among the 60 participants who completed the study, 15 (25%) screened positive for anxiety, 11 (18%) had subjective insomnia, and 34 (59%) had objective short sleep duration. In Model 1, increased anxiety was not associated with increases in any BP parameters. When subjective insomnia was included in Model 2, increased DBP and MAP was significantly associated with anxiety (DBP: β 6.1, p = 0.01, MAP: β 6.2 p

No campo da incorporação de tecnologias em saúde, há consenso no entendimento que a metodologia qualitativa é a adequada para abordar a informação colhida nos processos de engajamento social. Porém, a escolha de cada metodologia varia em função das características particulares de cada realidade. O presente artigo tem por finalidade apresentar uma combinação de análises qualitativas capazes de operacionalizar a participação social no contexto institucional da incorporação de tecnologias em saúde do Brasil e trazer subsídios para a tomada de decisões baseada em evidências científicas. Para a realização deste objetivo, seguimos o seguinte desenho: a) revisão de literatura; b) avaliação em detalhe dos formulários de experiência/opinião das Consultas Públicas, a fim de mensurar a sua adequação para a captação dos dados necessários para subsidiar as avaliações (o que poderá gerar propostas de modificação nos mesmos); c) realização de várias análises qualitativas do formulário de experiência e opinião das CPs em ATS, no marco da Comissão Nacional de Incorporação de Tecnologias no Sistema Único de Saúde brasileiro - Conitec, d) organização de um curso sobre metodologias qualitativas voltado para análises de CPs em ATS; e) processo de debate sobre as metodologias qualitativas mais adequadas para aplicar às Consultas Públicas. Entendemos que este é o método de análise que pode adequar-se às particularidades institucionais atuais e favorecer a participação social. Entretanto, resta ser completamente implementado e avaliado para poder revisar possíveis limitações.

Summary: Transport through the nuclear pore complex (NPC) relies on intrinsically disordered FG-nucleoporins (FG-Nups) forming a selective barrier. Away from the NPC, FG-Nups readily form condensates and aggregates, and we address how this behavior is surveilled in cells. FG-Nups, including Nsp1, together with the nuclear transport receptor Kap95, form a native daughter cell-specific cytosolic condensate in yeast. In aged cells, this condensate disappears as cytosolic Nsp1 levels decline. Biochemical assays and modeling show that Nsp1 is a modulator of FG-Nup condensates, promoting a liquid-like state. Nsp1’s presence in the cytosol and condensates is critical, as a reduction of cytosolic levels in young cells induces NPC defects and a general decline in protein quality control that quantitatively mimics aging phenotypes. These phenotypes can be rescued by a cytosolic form of Nsp1. We conclude that Nsp1 is a phase state regulator that surveils FG-Nups and impacts general protein homeostasis.