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The thermal pattern onto the TEXTOR-94 toroidal limiter at the thermal quench for a set of four disruptions has been measured. A high temporal and spatial resolution camera, based on an infra-red 128 x 128 pixels focal plane array detector has been used to perform the measurements. Comparing the power profile for the same discharge as inferred from two images taken just before the disruption and at the thermal quench, it is possible to assess that: the radial exponential decay of the power flowing onto the limiter shows in both cases two different e-folding lengths, a short one (of the order of 2 mm or even less, in disruptive discharges) in the first few mm of the SOL depth and a larger one (1.5 cm) deeper in the SOL, An energy balance has been performed based on thermal images of both the limiter and the nearby wall during the thermal quench. The total energy lost is comparable to the plasma energy content. (C) 1999 Elsevier Science B.V. All rights reserved.

A conserved hydrophobic region in the bilirubin-type UDP-glucuronosyltransferase isozyme was first uncovered as a consequence of a deleterious mutation in the UGT1A1 (HUG-Br1) isozyme of a Crigler-Najjar (CN) Type I patient. According to analysis by the RAOARGOS computer program, this hydrophobic region in UGT1A1 is located between residues 159-177 and defines a buried helix centered over position 169-172 with a positive factor of 1.22. Further analysis showed that the planar phenol-type UGT1A6 (HLUG P1) isoform, unlike the steroid-type UGT2B7 (UDPGTh2) isozyme, has a similar conserved hydrophobic region and that the positive factor for its buried helix is 1.14 compared to the threshold of 1.13 for such a structure. The analysis detected the typical membrane-insertion-signal sequence and a membrane-anchoring domain in each isoform. The different amino acid sequence patterns between positions 168-172 for the three types of isoforms and the deleterious mutations in this microregion (MRA) of UGT1A1 in CN-I patients are evidence of a critical and descriminating role for MRA. With the recombinant UGT1A1 enzyme and its mutants, P167G, F170del, F170L, F170I, F170V, F170A, F170Y, F170E, F171L, F171I, F171V, F171A, F171Y, or L175Q, expressed in COS-1 cells, bilirubin glucuronidating activity at both pH 6.4 and 7.6 demonstrated that Phe-170 is not replaceable, whereas Phe-171 can be replaced by Leu without any loss of activity. The less hydrophobic buried helix in the phenolic-type UGT1A6 has a Tyr/Leu at position 170/171; this isoform glucuronidated bilirubin at 1/10 the level of that by UGT1A1 with a Km (bilirubin) of 25 microM compared to that for UGT1A1 of 5. 0 microM.

Two missense mutations were uncovered in the UGT1A6 (HLUG P1) cDNA which codes for a human phenol-metabolizing UDP-glucuronosyltransferase. The mutant and a wild-type UGT1A6 cDNAs were isolated from a custom synthesized human liver lambda Zap cDNA library. Both an A to G transition at nucleotide 541 (T181 A) and an A to C transversion at nucleotide 552 (R184S) occurred in exon 1 of the UGT1A6 (UGT1F) gene at the UGT1 locus. The two mutations on a single allele created a heterozygous genotype. Newly created BsmI and BsoFI sites at the T181 A and R184S locations, respectively, were confirmed by endonuclease treatment of PCR-generated DNA using the donor-liver genomic DNA as template. Screens with endonuclease treatment showed that 33/98 DNA samples were heterozygous with both mutations on one allele. One other individual also carried the R184S mutation on the second allele. Wild-type UGT1A6 generated a broad plateau of activity from pH 5.0 to pH 8.0 with certain experimental phenols, while activity was 1.3-2.5-fold higher at pH 6.4 than at pH 7.2 for others. UGT1A6*2 (181 A+ and 184S+) metabolized 4-nitrophenol, 4-tert-butylphenol, 3-ethylphenol/4-ethylphenol, 4-hydroxycoumarin, butylated hydroxy anisole and butylated hydroxy toluene, with the pH 6.4 preference, at only 27-75% of the rate of the wild-type isozyme whereas 1-naphthol, 3-iodophenol, 7-hydroxycoumarin, and 7-hydroxy-4-methylcoumarin were metabolized at essentially the normal level. Furthermore, UGT1A6*2 metabolized 3-O-methyl-dopa and methyl salicylate at 41-74% of that of the wild-type, and a series of beta-blockers at 28-69% of the normal level. This evidence suggests that the UGT1A6 enzyme activity is affected by different amino acids depending upon the substrate selection.

Results are reported of the 8 GHz Lower Hybrid experiments on FTU after the installation of the new toroidal limiter. A figure of merit of the Current Drive efficiency eta(CD) approximate to 0.11.10(20) A/Wm(2) is estimated for plasma density = 10(20) m(-3) and no appreciable broadening of the launched frequency is detected. In low density experiments sawteeth are stabilised and m = 1 activity is present in the plasma. Shear reversed discharges with large reversal radius, r(S)/a approximate to 0.5, are obtained at higher density, lower temperature, B-T = 4 T, q(a) approximate to 5.5, by off-axis LH CD. The reversed configurations exhibit high central temperature coexisting with regular m = 2, n = 1 relaxations of large amplitude and are maintained up to LH switch off. At higher magnetic field, B = 5.2 T, q(a) approximate to 7, irregular DTM crashes are present during the whole LH pulse. Confinement time of radiofrequency heated discharges (P-LH = 0.5 divided by 2.P-OH) exhibits the same behaviour of FTU ohmic discharges following the ITER89-P scaling. Preliminary results of central 140 GHz Electron Cyclotron Resonant Heating (ECRH) during the plasma current ramp-up, aimed at obtaining shear reversed configurations are also reported.

The human major bilirubin UDP glucuronosyltransferase (transferase), HUG-Brl, and its mutants were expressed in the COS-1 cells using cDNA-based pSVL expression units to generate isoforms for the comparison of relative activities with 17 alpha-ethynlestradiol (17 alpha-EE) and bilirubin, its natural substrate. In comparison to bilirubin, 17 alpha-EE was a good substrate for HUG-Br1 under typical assay conditions of pH 7.2, confirming published studies [Ebner, T., et al. (1993) Mol. Pharmacol. 43, 649-654]. It was further shown that the estrogen derivative is 1.2-2-fold more effective as a substrate at pH 6.4 than at pH 7.2. The km for 17 alpha-EE was 40 microM under both pH conditions, while the Vmax values were 400 and 200 pmol per hour per 300 micrograms of protein at pH 6.4 and 7.2, respectively. The pattern of glucuronidation was similar for both bilirubin and 17 alpha-EE. Previously, a ratio of 2-3-fold more activity for bilirubin glucuronidation at pH 6.4 versus 7.6 was established, and km values of 2.5 microM at both pH conditions were determined [Ritter, J.K., et al. (1993) J. Biol. Chem. 268, 23573-23579]. In this study, the generation of 17 alpha-EE and bilirubin beta-glucuronides under both pH conditions was confirmed by the sensitivity of the products to beta-glucuronidase treatment. Concurrent glucuronidation reaction mixtures containing equal amounts of wild-type and mutant proteins demonstrated the following. P270G, V273D, and five different G276 mutants nearly or completely inactivated all glucuronidation at both pH levels. V273Q generated 81-94% of the normal activity for 17 alpha-EE and 42% of the normal activity for bilirubin turnover; H173R gave 37-60% of the normal turnover with both substrates, and V275I produced 15-24% of the normal level of glucuronide with both compounds. The most distinguishing amino acid tested was P176G which was approximately 50% normal for 17 alpha-EE at both pH conditions but was totally inactive for bilirubin. A second substitution, P285G, did not affect 17 alpha-EE turnover but was 50% normal for bilirubin. The parallel effects on the metabolism of both substrates by some mutants and the opposite results from two mutants are evidence for a common set of amino acids for their catalysis with the recruitment of additional amino acids to depend upon the substrate to be metabolized. Hence, amino acid substitutions in the protein are not necessarily universally inactivating.

Plasma behaviour in the high density regime has been investigated on the Frascati Tokamak Upgrade (FTU). The items particularly addressed are density limit, MARFE characteristics and fuelling efficiency. With gas puffing, a maximum line average density of 3.2*1020 m-3 has been obtained in the ohmic regime at q=5.1, corresponding to 1.7 times the Greenwald limit; while with pellet injection, a value of 3.7*1020 m-3 has been reached at q=3.5. The density limit appears to be connected with the impurity content and edge parameters, so the best results are obtained with very clean plasmas and peaked electron density profiles. The MARFE phenomenon always appears beyond a critical density that depends on the total input power and the effective charge: emissivities in the range of 2 to 12 MW/m3 have been measured in this highly radiating region. The fuelling efficiency, starting from a value of about 50% at low density, progressively deteriorates, falling to 10% near the density limit: this behaviour is interpreted as being due to a decrease of the scrape-off layer transparency to incoming neutrals

Three methods of density modulation have been used in FTU discharges (gas puffing, pellet injection and current rampup). The analysis of particle balance confirms that the neoclassical theory is not adequate and the experimental results obtained by the three methods are used to verify the validity of an empirical expression for the radial particle flux. Such an expression is relevant for the design of future experiments and constitutes a constraint for any satisfactory theory.

The characterization (Ritter, J. K., Chen, F., Sheen, Y. Y., Tran, H. M., Kimura, S., Yeatman, M. T., and Owens, I. S. (1992) J. Biol. Chem. 267, 3257-3261) of the single-copy UGT1 gene complex locus encoding both bilirubin and phenol UDP-glucuronosyltransferases (transferase) has been critical to the determination of genetic defects in Crigler-Najjar patients. The complex (UGT1A-UGT1M) codes for at least two bilirubin, three bilirubin-like, and eight phenol transferase isozymes. In the 5′ region, a minimum of 13 different exons 1, each with an upstream promoter, are arrayed in series with 4 common exons in the 3′ region of the locus. Each exon 1 encodes the amino terminus of a transferase, and the common exons encode the common carboxyl terminus of each isoform. Although a deleterious mutation in a common exon inactivates the entire locus, a deleterious mutation in an exon 1, as we report here for the UGT1A gene in a Crigler-Najjar Type I patient, affects the amino terminus of that single isoform. Recessively inherited mutant alleles for the predominant bilirubin isozyme, the HUG-Br1 protein, substituted Arg for Gly at codon 276 (G276R) in exon 1 of UGT1A abolishing a conserved di-glycine. The mutant HUG-Br1-G276R protein expressed in COS-1 cells had no detectable bilirubin glucuronidating activity at either pH 7.6 or 6.4. Although each of the bilirubin-type isozymes contains a conserved peptide between residues 270 and 288, all UDP-glucuronosyltransferases contain a di-glycine at approximately position 276-277, making it strictly conserved. Structure-function relationship was studied by site-directed mutations of the HUG-Br1 cDNA; G276A, G276Q, G276E, G276I, and P270G mutants were inactive, and V275I- and P285G-altered transferases expressed normal activity. Conservation of residues between the related baculoviral ecdysone UDP-glucosyltransferase and the UDP-glucuronosyltransferases confirms the critical role of the Gly-276 as well as other residues.

A simple 3D algorithm is used to minimise heat load variation on a plasma facing component for a given e-folding length of the power flowing in the scrape-off layer of a tokamak machine. Heat load peaking factors not larger than 2 are obtained for the geometry of the FTU machine. Problems arising when shadowing between different components is considered are discussed.

BACKGROUND: Because of the extreme genetic variability of hepatitis C virus (HCV), we analyzed whether specific HCV-genotypes are differently prone to develop resistance to linear and macrocyclic protease-inhibitors (PIs). METHODS: The study includes 1568 NS3-protease sequences, isolated from PI-naive patients infected with HCV-genotypes 1a (N = 621), 1b (N = 474), 2 (N = 72), 3 (N = 268), 4 (N = 54) 5 (N = 6), and 6 (N = 73). Genetic-barrier was calculated as the sum of nucleotide-transitions (score = 1) and/or nucleotide-transversions (score = 2.5) required for drug-resistance-mutations emergence. Forty-three mutations associated with PIs-resistance were analyzed (36A/M/L/G-41R-43S/V-54A/S/V-55A-Q80K/R/L/H/G-109K-138T-155K/Q/T/I/M/S/G/L-156T/V/G/S-158I-168A/H/T/V/E/I/G/N/Y-170A/T-175L). Structural analyses on NS3-protease and on putative RNA-models have been also performed. RESULTS: Overall, NS3-protease was moderately conserved, with 85/181 (47.0%) amino-acids showing 94% HCV-2-4; 175L present in 100% HCV-1a-3-5 and >97% HCV-2-4). Furthermore, HCV-3 specifically showed non-conservative polymorphisms (R123T-D168Q) at two drug-interacting positions. Regardless of HCV-genotype, 13 PIs resistance-mutations were associated with low genetic-barrier, requiring only 1 nucleotide-substitution (41R-43S/V-54A-55A-80R-156V/T: score = 1; 54S-138T-156S/G-168E/H: score = 2.5). By contrast, by using HCV-1b as reference genotype, nucleotide-heterogeneity led to a lower genetic-barrier for the development of some drug-resistance-mutations in HCV-1a (36M-155G/I/K/M/S/T-170T), HCV-2 (36M-80K-155G/I/K/S/T-170T), HCV-3 (155G/I/K/M/S/T-170T), HCV-4-6 (155I/S/L), and HCV-5 (80G-155G/I/K/M/S/T). CONCLUSIONS: The high degree of HCV genetic variability makes HCV-genotypes, and even subtypes, differently prone to the development of PIs resistance-mutations. Overall, this can account for different responsiveness of HCV-genotypes to PIs, with important clinical implications in tailoring individualized and appropriate regimens.

Variants of HPV types may have different oncogenic potential. While HPV 16 and 18 variants have been extensively studied, little is known on the less frequent high-risk types such as HPV 53 and 66. Here, we analyzed the genetic variability of HPV 53 and 66 by sequencing the E6, E7, L1 genes and the Long Control Region (LCR) sequences of HPV 53 and HPV 66 from infected women. Fisher's exact-test was performed to correlate viral variants with cervical lesions. Higher-order interactions among identified mutations were analyzed by co-variation and cluster analyses. Antigenic-index alterations following L1 mutations were predicted by Jameson-Wolf algorithm. In HPV53, novel variants were identified in L1 (N=9) and E6 (N=1) genes. The novel L1 mutation P432L was statistically associated with L-SIL lesions (P=0.04) and its development reduced the L1 predicted antigenicity (up to -2.3 for Glu433). HPV 53 E6 and L1 sequences clustered phylogenetically into two main clades. In HPV 66, novel polymorphisms were identified in L 1 (N=4) and E6 (N=4) genes. The L1 protein mutations S405P and D458N were exclusively found in patients with L-SILs. Seven E7 variants and 10 LCR variants were for the first time analyzed. Novel HPV 53 and 66 variants were identified in this study. Some of these mutations were significantly associated with L-SIL lesions and affected the antigenic index of the L1 protein with possible interesting implications in vaccine design.

Experiments with different dominant impurities (C, Si, Ni, Mo) have been carried out on the FTU tokamak. Density limit, marfe, Z(eff) and radiation losses are compared and discussed. An analytical model that couples in a self consistent way the parameters of the SOL and the transport of the impurities generated at the limiter to the bulk plasma parameters has been used to reproduce the experimental data.

Several years ago, our laboratory reported isolation of a virus, HTLV-5, from a Tacnegative T-cell lymphoma/leukemia. To date, however, no cell line has been obtained which consistently produces amounts of virus sufficient to permit detection of reverse transcriptase (RT) or to form the basis for a serologic test. In the process of attempting to develop such a cell line, we found that the lymphocyte cell line, GB, derived from a patient with cutaneous lymphoma/leukemia, produces an inhibitor of RT. Production of this inhibitory factor is transmitted, both by infection and by transfection, to cell lines which do not produce it spontaneously.

The possibility of applying H2 glow discharge cleaning for the conditioning of the FTU vacuum chamber has been analyzed on a 1:1 scale toroidal sector. The optimization of the surface conditioning acquires relevant importance because of the complexity the inner wall geometry. The FTU vacuum chamber is completely covered by thermal shields that originate hidden regions not exposed to the discharge. Thus, the best procedure to clean both the exposed and non exposed surfaces simultaneously is the combined operation of baking plus glow discharge.

s A.I.S.F. Annual Meeting (Rome, February 25th−26th, 2010) / Digestive and Liver Disease 42 Suppl. 1 (2010) S1–S51 S11 lamivudine had been withdrawn (since 8−32 weeks): only one however remained HBsAg-positive and was given tenofovir, with HBV-DNA negativization after 4 weeks. This was the only patient testing positive for total and ccc-DNA in the third liver biopsy. In the remaining 3 (who negative at the third biopsy) HBsAg positivity was transient and followed by anti-HBs seroconversion, with titers ranging from 25 to 80 IU/L after a median follow-up of 6 weeks (range = 4−8). There was no need of antiviral treatment in these patients. Conclusions: The large majority of patients with undetectable HBV viremia at transplant have no evidence of intrahepatic total and cccDNA, and may safely undergo cautious weaning of HBIg and lamivudine. A minority of them may also undergo anti-HBs seroconversion. A more extended follow-up of these patients is needed before this strategy can be considered for clinical practice. 26 PLATELET COUNT/SPLEEN DIAMETER RATIO COMPARED TO HVPG AND TRANSIENT ELASTOGRAPHY TO PREDICT SEVERE PORTAL HYPERTENSION IN PATIENTS WITH LIVER CIRRHOSIS W. De Bernardi Venon1, D. Bitetto2, C. Fabris2, P. Toniutto2, M. Rizzetto1, A. Marzano1. Gastroenterology, University of Torino, Medical Liver Transplant Unit, DPMSC, University of Udine, Italy Background and Aim: Portal hypertension (PHT) is defined as a hepatic venous pressure gradient (HVPG) >5mmHg. HVPG

a Department of Public Health and Microbiology, Medical School of Turin, Turin, Italy b Department of Clinical and Experimental Medicine, Medical School of Novara, Novara, Italy c Department of Laboratory Medicine, Laboratory of Clinical Microbiology and Virology, University of Rome Tor Vergata, Rome, Italy d Department of Biopathology and Image Diagnostics, University of Rome Tor Vergata, Rome, Italy e Department of Biomedical Sciences and Human Oncology, Section of Pathology, Medical School of Turin, Turin, Italy

Background Human papillomaviruses (HPVs) are the primary etiological agents of cervical cancer and are also involved in the development of other tumours (skin, head and neck). Serological survey of the HPV infections is important to better elucidate their natural history and to disclose antigen determinants useful for vaccine development. At present, the analysis of the HPV-specific antibodies has not diagnostic value for the viral infections, and new approaches are needed to correlate the antibody response to the disease outcome. The aim of this study is to develop a novel ELISA, based on five denatured recombinant HPV16 proteins, to be used for detection HPV-specific antibodies. Methods The HPV16 L1, L2, E4, E6 and E7 genes were cloned in a prokaryotic expression vector and expressed as histidine-tagged proteins. These proteins, in a denatured form, were used in ELISA as coating antigens. Human sera were collected from women with abnormal PAP smear enrolled during an ongoing multicenter HPV-PathogenISS study in Italy, assessing the HPV-related pathogenetic mechanisms of progression of cervical cancer precursor lesions. Negative human sera were collected from patients affected by other infectious agents. All the HPV-positive sera were also subjected to an avidity test to assess the binding strength in the antigen-antibody complexes. Results Most of the sera showed a positive reactivity to the denatured HPV16 proteins: 82% of the sera from HPV16 infected women and 89% of the sera from women infected by other HPV genotypes recognised at least one of the HPV16 proteins. The percentages of samples showing reactivity to L1, L2 and E7 were similar, but only a few serum samples reacted to E6 and E4. Most sera bound the antigens with medium and high avidity index, suggesting specific antigen-antibody reactions. Conclusion This novel ELISA, based on multiple denatured HPV16 antigens, is able to detect antibodies in women infected by HPV16 and it is not genotype-specific, as it detects antibodies also in women infected by other genital HPVs. The assay is easy to perform and has low cost, making it suitable for monitoring the natural history of HPV infections as well as for detecting pre-existing HPV antibodies in women who receive VLP-based HPV vaccination.

The radiative improved mode obtained on the limiter tokamak TEXTOR-94 combines the possibility of power exhaust by a radiating plasma boundary (with a fraction of the radiated power with respect to the total input power up to 90% with neon or argon cooling) with improved energy confinement (as good as in the ELM-free H-mode in divertor tokamaks) at high plasma densities (line-averaged central-electron density equal to or even above the Greenwald density limit ) in quasi-stationary discharges. An overview is given of the substantial changes in plasma-edge properties occurring at high radiated power levels . These changes are characterized by a reduction of the plasma-edge density and temperature, a reduction of particle transport out of the confined plasma volume and an increase of the penetration depth of deuterium and impurity atoms. As a consequence, the particle confinement time increases and the electron-density profiles steepen. The transition to improved confinement takes place as soon as the density peaking reaches a critical threshold. An internal transport barrier is observed in the bulk of RI-mode plasmas (at ) characterized by an increase of the pressure gradient and of the shear of the toroidal velocity compared to discharges without additional impurity seeding. The dilution at the plasma boundary is strongly increased by the seeded impurities whereas the central dilution is only weakly affected.

Thermo-hydraulic modeling of cable in conduit conductors (CICC) contains one main uncertainty in the heat exchange coefficient used in the different possible fluid regimes. Nowadays validation of codes, by comparison with data from experiments involving long length instrumented conductors, is one of the main technique for assessing the design tools for the large magnets foreseen in the next generation fusion machines. To this purpose, a broad disturbance scenario has to be investigated to confirm the magnet's stability versus normal and off-normal working conditions. In this paper a comparison between the predictions of the code Gandalf and the data obtained from an instrumented NbTi conductor module is carried out.

Background Continuous improvements in next generation sequencing technologies led to ever-increasing collections of genomic sequences, which have not been easily characterized by biologists, and whose analysis requires huge computational effort. The classification of species emerged as one of the main applications of DNA analysis and has been addressed with several approaches, e.g., multiple alignments-, phylogenetic trees-, statistical- and character-based methods. Results We propose a supervised method based on a genetic algorithm to identify small genomic subsequences that discriminate among different species. The method identifies multiple subsequences of bounded length with the same information power in a given genomic region. The algorithm has been successfully evaluated through its integration into a rule-based classification framework and applied to three different biological data sets: Influenza, Polyoma, and Rhino virus sequences. Conclusions We discover a large number of small subsequences that can be used to identify each virus type with high accuracy and low computational time, and moreover help to characterize different genomic regions. Bounding their length to 20, our method found 1164 characterizing subsequences for all the Influenza virus subtypes, 194 for all the Polyoma viruses, and 11 for Rhino viruses. The abundance of small separating subsequences extracted for each genomic region may be an important support for quick and robust virus identification. Finally, useful biological information can be derived by the relative location and abundance of such subsequences along the different regions. Electronic supplementary material The online version of this article (doi:10.1186/s13040-016-0116-2) contains supplementary material, which is available to authorized users.

Human papillomavirus (HPV) has been found in lung cancer cases with variable frequency. In the present study, we analysed a series of 38 patients with non-small cell lung cancer (NSCLC) (21 paraffin-embedded archival samples and 17 fresh surgical specimens) for the presence of E6 and E7 oncogenes of HPV16, 18 and 31. Eight of the tumours were positive (21%): six HPV16, one HPV16+18, and one HPV31. The normal tissue surrounding the HPV-positive tumour was negative for the presence of the virus. Sequencing analysis of URR, of HPV16, which was the most frequently found HPV type in our cases, showed an adenosine deletion at nucleotide 7861 (E2-binding site) in four out of six patients. Sequencing of the entire E6 and E7 genes of HPV16 showed a T to G transition at nucleotide position 350 of E6, in all examined cases. This mutation is associated to the European variant of HPV16. Analysis of E6 and E7 transcripts was performed on the six fresh surgical specimens infected by HPV16. Our study showed that all of the tumours investigated, except one, contained E6 and E7 transcripts. Only in one case could we identify an unspliced form of the E6 transcript. Our results strengthen the relationship between HPV and NSCLC and support the hypothesis that HPV infection could play a role in bronchial carcinogenesis.

Within the framework of the R&D studies for the International Thermonuclear Experimental Reactor (ITER) project, the first full size NbTi conductor sample was fabricated industrially and tested in the SULTAN facility (Villigen, Switzerland). This sample (PF-FSJS), which is relevant to the poloidal field coils of ITER, is composed of two parallel straight bars of conductor, connected at the bottom through a joint designed according to the CEA twin-box concept. The two conductor legs are identical except for the use of different strands: a nickel plated NbTi strand with a pure copper matrix in one leg, and a bare NbTi strand with a copper matrix and internal CuNi barrier in the other leg. The two conductors and the joint were extensively tested as regards DC and AC properties. This paper reports on the test results and analysis, stressing the differences between the two conductor legs and discussing the impact of the test results on the ITER design criteria for the conductor and joint. While joint DC resistance, conductors and joint AC losses fulfilled the ITER requirements, neither conductor could reach its current sharing temperature at relevant ITER currents, due to instabilities. Although the drop in temperature is slight for the CuNi strand cable, it is more significant for the Ni plated strand cable.

Background Carbapenemase-producing Enterobacteriaceae (CPE), particularly carbapenemase-producing Klebsiella pneumoniae isolates, are important causative agents of nosocomial infections associated with significant mortality rates mostly in critical wards. The rapid detection and typing of these strains is critical either for surveillance purposes and to prevent outbreaks and optimize antibiotic therapy. In this study, the MALDI-TOF MS method was used to detect rapidly these isolates from blood cultures (BCs) and to obtain proteomic profiles enable to discriminate between carbapenemase-producing and non-carbapenemase-producing strains. Results Fifty-five K. pneumoniae strains were tested. Identification and carbapenemase-production detection assay using Ertapenem were performed both from bacterial pellets extracted directly from BCs flasks and from subcultures of these strains. For all isolates, a complete antimicrobial susceptibility testing and a genotypic characterization were performed. We found 100% agreement between the carbapenemase-producing profile generated by MALDI TOF MS and that obtained using conventional methods. The assay detected and discriminated different carbapenemase-producing K. pneumoniae isolates within 30 min to 3 h after incubation with Ertapenem. Conclusions MALDI-TOF MS is a promising, rapid and economical method for the detection of carbapenemase-producing K. pneumoniae strains that could be successfully introduced into the routine diagnostic workflow of clinical microbiology laboratories.

Limited information is available on the viral aetiology of influenza-like illness (ILI) in Southern European countries. Hereby we report the main findings of a survey conducted in the area of Rome during the 2004-2005 winter season. ILI cases were defined as individuals with fever >37.5°C and at least one constitutional symptom and one respiratory symptom, recruited during the survey period. Influenza and other respiratory viruses were identified using polymerase chain reaction (PCR) on throat swabs. Basic individual information was collected through a standard form. Of 173 ILI cases enrolled, 74 tested positive for one virus, and two tested positive for two viruses. Overall, 33.5% of the cases were positive for influenza viruses, 5.2% for adenoviruses, 3.5% for parainfluenza viruses, 1.7% for coronaviruses, and 1.2% for the respiratory syncitial virus. The proportion of influenza virus detection was higher in the ‘high influenza activity’ period. The distribution of viral agents varied across age groups, influenza viruses being more likely to be detected in younger patients. Viral pathogens were identified in less than 50% of ILI cases occurred during a high activity influenza season. The detection of other than influenza viruses was sporadic, without evidence of large outbreaks due to specific agents.

The MHD activity of plasma configurations with reversed magnetic shear has been investigated on the FTU tokamak. In the presence of pairs of surfaces with the same rational value q = m/n of the safety factor, double-tearing modes are excited which give rise in most cases to bursts of sawtooth-like profile rearrangements. More stable regimes have also been found, in which the activity is dominated by rotating saturated modes. In a particular case with q(0) approximate to 2.8 and q(min) approximate to 2.5 a discharge without any detectable MHD activity during the current flat-top has been obtained. In high-temperature regimes (T-e x 8 keV at n(e) approximate to 0.8 x 10(20) m(-3)), an irregular activity has been detected near the plasma centre which could be due to the excitation of resistive interchange modes.

Background Differences in genomic sequences are crucial for the classification of viruses into different species. In this work, viral DNA sequences belonging to the human polyomaviruses BKPyV, JCPyV, KIPyV, WUPyV, and MCPyV are analyzed using a logic data mining method in order to identify the nucleotides which are able to distinguish the five different human polyomaviruses. Results The approach presented in this work is successful as it discovers several logic rules that effectively characterize the different five studied polyomaviruses. The individuated logic rules are able to separate precisely one viral type from the other and to assign an unknown DNA sequence to one of the five analyzed polyomaviruses. Conclusions The data mining analysis is performed by considering the complete sequences of the viruses and the sequences of the different gene regions separately, obtaining in both cases extremely high correct recognition rates.