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• The mode-locking function of In 2 S 3 was experimentally demonstrated for the first time. • The 2nd to 11st harmonics were realized in 2-μm region. • The maximum harmonic-resonance frequency of 459 MHz that has ever been demonstrated in a In 2 S 3 -based fiber laser. There are few reports on the mode-locking operation of indium sulfide (In 2 S 3) in fiber lasers. In this paper, we experimentally demonstrate a harmonic mode-locked fiber laser in 2-μm region by taking advantage of an In 2 S 3 -based saturable absorber (SA). The In 2 S 3 -based SA has nonlinear optical modulation depth of 20.8 % and saturation power of 0.14 kW at 1930 nm. The fundamentally mode-locked solitons at 1938.59 nm with the 3-dB bandwidth of 5.04 nm, pulse width of 1.16 ps, and pulse repetition rate of 41.73 MHz are generated. The maximum harmonic-resonance frequency is 459 MHz (11th harmonic), which is the highest operating frequency that has been demonstrated in In 2 S 3 -based mode-locked fiber lasers as far as we know. The spectral and temporal characteristics of the output pulses are investigated versus harmonic order. The results prove that In 2 S 3 possesses the characteristics of mode-lockers and could be a promising optical switch for ultrafast pulses generation. [ABSTRACT FROM AUTHOR]

• There was synergistic effect between SL/CG/CCG co-pyrolysis. • SL/CCG co-pyrolysis increased the biochar yield. • The physical and chemical properties of coal gangue are improved after calcination treatment. • The addition of modified coal gangue can increase the yield of biochar under high temperature (700°C) conditions. In this work, SEM-EDS and FTIR were first used to analyze the physical and chemical characterization of salix (SL), the raw coal gangue (CG)/calcined coal gangue (CCG) and the biochar. Then the pyrolysis characteristics of SL, CG/CCG and their mixtures were explored by thermogravimetric analysis (TGA). The research results showed that the reactivity of CG was improved after calcination treatment. Compared with CG blended SL, the co-pyrolysis of CCG and SL had a significant synergistic effect. There was a strong synergistic effect when SL blended with 30% CG. When SL blended with 40% CCG and the pyrolysis temperature was higher than 530°C, the adsorption performance of biochar had been significantly improved, and the addition of CCG that obviously promotes the carbon-forming reaction of SL and the biochar yield increased by 5.43%. [ABSTRACT FROM AUTHOR]

• Experimental demonstration of mode-locked fiber laser based on Sb 2 Se 3 saturable absorber for the first time. • Harmonic mode-locking from the 2nd to the 31st order in 2 μm waveband. • The highest operating frequency of 693 MHz that has ever been demonstrated in a Sb 2 Se 3 -based fiber laser. Studies have shown that Sb 2 Se 3 had the potential to be used as an optical switch or a mode-locker. However, the actual performance of its optical switch capability in fiber lasers has not been proved yet. In this paper, we experimentally demonstrate the nonlinear optical modulation characteristics and optical switch capabilities of Sb 2 Se 3 with a harmonic mode-locked thulium-doped fiber laser (TDFL). The nonlinear modulation depth and saturation power of Sb 2 Se 3 -based SA at 1930 nm are 9.3% and 4.08 kW, respectively. The fundamentally mode-locked pulses operate at the central wavelength of 1961.35 nm have the 3-dB bandwidth of 4.57 nm, repetition rate of 22.36 MHz and pulse width of 890 fs. The 2th to 31th harmonic mode locking (HML) are observed, corresponding to the pulse repetition rates ranging from 44.7 to 693 MHz. To the best of our knowledge, this is the highest operating frequency that has ever been demonstrated in a Sb2Se3-based fiber laser. The experimental results indicate that Sb 2 Se 3 could be a promising candidate for generating ultrashort pulses with high repetition rates. [ABSTRACT FROM AUTHOR]

• Mode-locking operation at 1963 nm in a Tm-doped fiber laser. • SnSe nanoparticles deposited on a D-shaped fiber as a saturable absorber. • Harmonic mode-locking from the second order to the eighth order. • Repetition rate of 24.59 MHz and pulse width of 1.2 ps. In this paper, tin selenide (SnSe) nanoparticles (NPs) are employed as a saturable absorber (SA) in a passively mode-locked thulium (Tm)-doped fiber laser for the first time. The mode-locked pulse has a central wavelength of 1963 nm and a 3-dB bandwidth of 4.3 nm. The repetition rate is 24.59 MHz and the full width at half maximum (FWHM) of the pulse is 1.2 ps. Harmonics from the second order to the eighth order are observed. Our results prove that the SnSe have excellent nonlinear saturable absorption properties and could have wide potential applications on ultrafast photonics in mid-infrared waveband. [ABSTRACT FROM AUTHOR]

Lecithin cholesterol acyltransferase (LCAT), a crucial enzyme in lipid metabolism, plays important yet poorly understood roles in tumours, especially in hepatocellular carcinoma (HCC). In this study, our investigation revealed that LCAT is a key downregulated metabolic gene and an independent risk factor for poor prognosis in patients with HCC. Functional experiments showed that LCAT inhibited HCC cell proliferation, migration and invasion. Mechanistically, LCAT interacts with caveolin-1 (CAV1) to promote the binding of CAV1 to PRKACA and inhibit its phosphorylation, thereby inhibiting triglyceride (TAG) catabolism. On the other hand, LCAT inhibits fatty acid oxidation (FAO) by interacting with CPT1A to promote its ubiquitination and degradation. These events result in an inadequate supply of raw materials and energy and inhibit the malignant behaviours of HCC cells. In addition, LCAT is a reliable predictive biomarker for the efficacy of lenvatinib treatment in HCC patients, and the inhibition of FAO can increase lenvatinib sensitivity in patients with LCAT low HCC. This study revealed that LCAT plays a critical role in the regulation of lipid metabolic reprogramming and is a reliable predictive biomarker for the efficacy of lenvatinib treatment in HCC patients., Competing Interests: Declaration of competing interest The authors declare that they have no competing interests., (Copyright © 2025. Published by Elsevier B.V.)

Powdery mildew, caused by Blumeria graminis f. sp. tritici ( Bgt ), is a disease that seriously harms wheat production and occurs in all wheat-producing areas around the world. Exploring Pm gene(s) and developing resistant cultivars are preferred to control the disease. Wild emmer wheat ( Triticum dicoccoides , 2n = 4x = 28, AABB) has accumulated abundant gene resources for resistance to powdery mildew during the long process of natural evolution. In the current study, the WEW accession D430 was highly resistant to powdery mildew at the whole-growth stage. Genetic analysis showed that the powdery mildew resistance in D430 was conferred by a single dominant locus or gene by the cross of D430 and susceptible durum wheat 647, tentatively named PmD430 . Combining BSR-Seq analysis, molecular mapping, and sequence alignment, PmD430 was finally mapped to Pm4 locus, and the sequence was identical to Pm4b . Subsequently, 1871 DEGs between resistant and susceptible bulks were annotated and analyzed by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment. Eight disease-related genes were evaluated by qRT-PCR and exhibited a unique expression pattern when invaded by Bgt isolate E09 and was, therefore, presented as latent targets for regulating powdery mildew resistance in D430.

Increasing evidence highlights that fibroblast growth factor receptor 2 (FGFR2) fusion/rearrangement shows important therapeutic value for patients with intrahepatic cholangiocarcinoma (ICC). This study aims to explore the association of FGFR2 status with the prognosis and immune cell infiltration profiles of patients with ICC. A total of 226 ICC tissue samples from patients who received surgery at the Department of Liver Surgery at Zhongshan Hospital, Fudan University, were collected retrospectively and assigned to a primary cohort (n = 152) and validation cohort (n = 74) group. Fluorescence in situ hybridization was performed to determine FGFR2 status. Multiplex immunofluorescence (mIF) staining and immunohistochemistry were performed to identify immune cells. Thirty-two (14.2%) ICC tissues presented with FGFR2 fusion/rearrangement. FGFR2 fusion/rearrangement was associated with low levels of carcinoembryonic antigen (CEA, P = .026) and gamma glutamyl transferase (γ-GGT, P = .003), low TNM (P = .012), CNLC (P = .008) staging as well as low tumor cell differentiation (P = .016). Multivariate COX regression analyses revealed that FGFR2 fusion/rearrangement was an independent protective factor for both overall survival (OS) and relapse-free survival in patients with ICC. Furthermore, correlation analysis revealed that an FGFR2 fusion/rearrangement was associated with low levels of Tregs and N2 neutrophils and high levels of N1 neutrophils infiltrating into tumors but not with CD8+ T-cell or macrophage tumor infiltration. FGFR2 fusion/rearrangement may exert a profound impact on the prognosis of ICC patients and reprogram the tumor microenvironment to be an immune-activated state. FGFR2 status may be used for ICC prognostic stratification and as an immunotherapeutic target in patients with ICC., (© The Author(s) 2024. Published by Oxford University Press.)

Radix Astragali (RA) has been recognized for its therapeutic potential in allergic rhinitis (AR), yet its potential pharmacological mechanisms remain elusive. This study systematically investigated the physicochemical properties and biological activities of RA's phytochemicals, aiming to elucidate their targets and mechanisms in AR treatment. We identified 775 potential targets of RA's key phytochemicals and intersected these with 29,544 AR-related disease targets, pinpointing 747 shared therapeutic targets. A protein-protein interaction network analysis categorized these targets into five subclusters, with TNF, NFKB1, IKBKB, NFKBIA, and CHUK emerging as central nodes. Enrichment analysis revealed their roles in inflammatory and immune responses, particularly through the NF-κB, TNF, IL-17, Toll-like receptor, and NOD-like receptor signaling pathways. Molecular docking and dynamics simulations confirmed the strong binding affinity and stability of RA's phytochemicals to these targets. In vivo, RA intervention effectively reversed the expression of key inflammatory markers in an IL-13-induced nasal mucosa inflammation model. Our findings suggest that RA's multitargeted approach involves the modulation of critical inflammatory pathways, highlighting its therapeutic potential., Competing Interests: Declarations. Competing interests: The authors declare no competing interests., (© 2024. The Author(s).)

Background: Immune checkpoint inhibitors (ICIs) are therapeutically effective for hepatocellular carcinoma (HCC) but are individually selective. This study examined the role of specific common fragile sites (CFSs) related gene in HCC immunotherapy., Methods: We analyzed HCC tissues using next-generation sequencing and flow cytometry via time-of-flight technology. A humanized orthotopic HCC mouse model, an in vitro co-culture system, untargeted metabolomics and a DNA pulldown assay were used to examine the function and mechanism of WWOX in the tumor immune response., Results: WWOX was the most upregulated CFS-related gene in HCC patients responsive to ICIs. WWOX deficiency renders HCC resistant to PD-1 treatment in humanized orthotopic HCC mouse model. Macrophage infiltration is increased and CD8 T-cell subset infiltration is decreased in WWOX-deficient HCC patients. HCC-derived oleic acid (OA) promotes macrophage conversion to an immunosuppressive phenotype. Mechanistically, WWOX deficiency promoted OA synthesis primarily via competitive binding of NME2 with KAT1, which promoted acetylation of NME2 at site 31 and inhibited NME2 binding to the SCD5 promoter region. Pharmacological blockade of SCD5 enhanced the antitumor effects of anti-PD-1 therapy., Conclusions: WWOX is a key factor for immune escape in HCC patients, which suggests its use as a biomarker for stratified treatment with ICIs in clinical HCC patients., Competing Interests: Competing interests: None declared., (© Author(s) (or their employer(s)) 2024. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.)

Immune checkpoint inhibitors have limited efficacy in hepatocellular carcinoma (HCC). Macrophages are the most abundant immune cells in HCC, suggesting that a better understanding of the intrinsic processes by which tumor cells regulate macrophages could help identify strategies to improve response to immunotherapy. As signaling lymphocytic activation molecule (SLAM) family members regulate various immune functions, we investigated the role of specific SLAM receptors in the immunobiology of HCC. Comparison of the transcriptomic landscapes of immunotherapy-responsive and nonresponsive patients with advanced HCC identified SLAMF7 upregulation in immunotherapy-responsive HCC, and patients with HCC who responded to immunotherapy also displayed higher serum levels of SLAMF7. Loss of Slamf7 in liver-specific knockout mice led to increased hepatocarcinogenesis and metastasis, elevated immunosuppressive macrophage infiltration, and upregulated PD-1 expression in CD8+ T cells. HCC cell-intrinsic SLAMF7 suppressed MAPK/ATF2-mediated CCL2 expression to regulate macrophage migration and polarization in vitro. Mechanistically, SLAMF7 associated with SH2 domain-containing adaptor protein B (SHB) through its cytoplasmic 304 tyrosine site to facilitate the recruitment of SHIP1 to SLAMF7 and inhibit the ubiquitination of TRAF6, thereby attenuating MAPK pathway activation and CCL2 transcription. Pharmacological antagonism of the CCL2/CCR2 axis potentiated the therapeutic effect of anti-PD-1 antibody in orthotopic HCC mouse models with low SLAMF7 expression. In conclusion, this study highlights SLAMF7 as a regulator of macrophage function and a potential predictive biomarker of immunotherapy response in HCC. Strategies targeting CCL2 signaling to induce macrophage repolarization in HCC with low SLAMF7 might enhance the efficacy of immunotherapy., Significance: CCL2 upregulation caused by SLAMF7 deficiency in hepatocellular carcinoma cells induces immunosuppressive macrophage polarization and confers resistance to immune checkpoint blockade, providing potential biomarkers and targets to improve immunotherapy response in patients., (©2024 American Association for Cancer Research.)

Background: The efficacy of immune checkpoint inhibitors (ICIs) in hepatocellular carcinoma (HCC) is poor and great heterogeneity among individuals. Chemokines are highly correlated with tumor immune response. Here, we aimed to identify an effective chemokine for predicting the efficacy of immunotherapy in HCC., Methods: Chemokine C-C motif ligand 21 (CCL21) was screened by transcriptomic analysis in tumor tissues from HCC patients with different responses to ICIs. The least absolute shrinkage and selection operator (LASSO) regression analysis was conducted to construct a predictive nomogram. Neutrophils in vitro and HCC subcutaneous tumor model in vivo were applied to explore the role of CCL21 on the tumor microenvironment (TME) of HCC., Results: Transcriptome analysis showed that CCL21 level was much higher in HCC patients with response to immunotherapy. The predictive nomogram was constructed and validated as a classifier. CCL21 could inhibit N2 neutrophil polarization by suppressing the activation of nuclear factor kappa B (NF-κB) pathway. In addition, CCL21 enhanced the therapeutic efficacy of ICIs., Conclusion: CCL21 may serve as a predictive biomarker for immunotherapy response in HCC patients. High levels of CCL21 in TME inhibit immunosuppressive polarization of neutrophils. CCL21 in combination with ICIs may offer a novel therapeutic strategy for HCC., (© 2024. The Author(s).)

Background: Relapse of hepatocellular carcinoma (HCC) due to vascular invasion is common, but the genomic mechanisms remain unclear, and molecular determinants of high-risk relapse cases are lacking. We aimed to reveal the evolutionary trajectory of microvascular invasion (MVI) and develop a predictive signature for relapse in HCC., Methods: Whole-exome sequencing was performed on tumor and peritumor tissues, portal vein tumor thrombus (PVTT), and circulating tumor DNA (ctDNA) to compare the genomic profiles between 5 HCC patients with MVI and 5 patients without MVI. We conducted an integrated analysis of exome and transcriptome to develop and validate a prognostic signature in two public cohorts and one cohort from Zhongshan Hospital, Fudan University., Results: Shared genomic landscapes and identical clonal origins among tumor, PVTT, and ctDNA were observed in MVI ( +) HCC, suggesting that genomic changes favoring metastasis occur at the primary tumor stage and are inherited in metastatic lesions and ctDNA. There was no clonal relatedness between the primary tumor and ctDNA in MVI ( - ) HCC. HCC had dynamic mutation alterations during MVI and exhibited genetic heterogeneity between primary and metastatic tumors, which can be comprehensively reflected by ctDNA. A relapse-related gene signature named RGS HCC was developed based on the significantly mutated genes associated with MVI and shown to be a robust classifier of HCC relapse., Conclusions: We characterized the genomic alterations during HCC vascular invasion and revealed a previously undescribed evolution pattern of ctDNA in HCC. A novel multiomics-based signature was developed to identify high-risk relapse populations., (© 2023. Asian Pacific Association for the Study of the Liver.)

Objective: To conduct a retrospective analysis of Hemoporfin photodynamic therapy (HMME-PDT) in the treatment of port-wine stains (PWS)., Method: A retrospective analysis was conducted based on the clinical data from March 2017 to December 2022, so as to summarize the demographic characteristics, clinical efficacy and adverse reactions. The effectiveness of HMME-PDT was examined with respect to treatment times, age, gender, subtype, and location of PWS lesions., Result: The age of the 2952 cases ranged from 8 months to 56 years old (median, 2.8 years), with 1419 males (48.07 %), and 1533 females (51.93 %). There were 669 cases of pink type (22.66 %), 2184 cases of purplish red type (73.98 %), and 99 cases of nodular thickening type (3.35 %). The prevalence location was face (88.04 %), neck (14.94 %), limbs and trunk. 1602 cases (54.27 %) had never received treatment, 661 cases (22.39 %) had been treated by pulse dye laser (PDL), 229 cases (7.76 %) had previously been treated by PDT, 296 cases (10.03 %) had received both the modalities. The 2952 cases completed totally 7996 HMME-PDT times. Cure rate and effective rate increased continuously with the number of treatments. The pink type has the highest cure rate and effective rate, followed by the purplish red type and the last was the nodular thickening type. The therapeutic effects are considerably influenced by age, subtype, and treatment site (P < 0.05). However, there was no significant difference in the effectiveness of HMME-PDT between both genders. The local adverse reactions after the first treatment included edema (97.73 %), itching (82.62 %), purpura-like change (79.51 %), crusts (24.59 %), infection (4.07 %), scars (1.08 %), hyperpigmentation (0.61 %), and depigmentation (0.41 %). Nausea and vomiting occurred in 2 juveniles and 1 young adult (5, 6 and 22 years old respectively) immediately after treatment, and did not interfere with the administration of the treatment. Patients aged 21-30 were found to have a 3.4-fold higher likelihood of undergoing HMME-PDT under general anesthesia compared to those aged 15 or younger. There was no distinct systemic adverse reaction, such as allergic responses, cardiovascular effects, neurological symptoms, hematological abnormalities, respiratory symptoms, or musculoskeletal issues., Conclusion: HMME-PDT is preferred in treating PWS, with relatively high effective rate and cure rate, mild local reactions and no distinct systemic adverse reaction., Competing Interests: Declaration of Competing Interest The authors have no conflict of interest to declare, (Copyright © 2023. Published by Elsevier B.V.)

Background: Hepatocellular carcinoma (HCC) is a malignancy with limited treatment options and poor prognosis. Macrophages are enriched in the HCC microenvironment and have a significant impact on disease progression and therapy efficacy. We aim to identify critical macrophages subsets involved in HCC development., Methods: Macrophage-specific marker genes were identified through single-cell RNA sequencing analyses. The clinical significance of macrophages with palmitoyl-protein thioesterase 1 (PPT1) positive was investigated in 169 patients with HCC from Zhongshan Hospital using immunohistochemistry and immunofluorescence. The immune microenvironment of HCC and the functional phenotype of PPT1 + macrophages were explored using cytometry by time-of-flight (CyTOF) and RNA sequencing., Results: Single-cell RNA sequencing analyses revealed that PPT1 was predominantly expressed in macrophages in HCC. Intratumoral PPT1 + macrophages abundance was associated with inferior survival durations of patients and an independent risk factor of prognosis for HCC. High throughput analyses of immune infiltrates showed that PPT1 + macrophage-enriched HCCs were characterized by high infiltration of CD8 + T cells with increased programmed death-1 (PD-1) expression. PPT1 + macrophages exhibited higher galectin-9, CD172a, and CCR2 levels but lower CD80 and CCR7 levels than PPT1 - macrophages. Pharmacological inhibition of PPT1 by DC661 suppressed mitogen-activated protein kinase (MAPK) pathway activity but activated nuclear factor kappa B (NF-κB) pathway in macrophages. In addition, DC661 enhanced the therapeutic efficacy of anti-PD-1 antibody in the HCC mouse model., Conclusions: PPT1 is mainly expressed in macrophages in HCC and promotes immunosuppressive transformation of macrophages and tumor microenvironment. PPT1 + macrophage infiltration is associated with poor prognosis of patients with HCC. Targeting PPT1 may potentiate the efficacy of immunotherapy for HCC., Competing Interests: Competing interests: None declared., (© Author(s) (or their employer(s)) 2023. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.)

The tumor microenvironment (TME) plays a crucial role in tumor initiation, growth and metastasis. Metabolic enzymes involved in tumor glycolytic reprogramming, including hexokinase, pyruvate kinase, and lactate dehydrogenase, not only play key roles in tumorigenesis and maintaining tumor cell survival, but also take part in the modulation of the TME. Many studies have been devoted to the role of key glycolytic enzymes in the TME over the past decades. We summarize the studies on the role of glycolytic enzymes in the TME of these years and found that glycolytic enzymes remodel the TME primarily through regulating immune escape, angiogenesis, and affecting stromal cells and exosomes. Notably, abnormal tumor vascular system, peritumoral stromal cells, and tumor immunosuppressive microenvironment are important contributors to the failure of antitumor therapy. Therefore, we discuss the mechanisms of regulation by key glycolytic enzymes that may contribute to a promising biomarker for therapeutic intervention. We argue that targeting key glycolytic enzymes in combination with antiprogrammed cell death ligand 1 or antivascular endothelial growth factor could emerge as the more integrated and comprehensive antitumor treatment strategy.

Background & Aims: The therapeutic effect of immune checkpoint inhibitors (ICIs) is poor in hepatocellular carcinoma (HCC) and varies greatly among individuals. Schlafen (SLFN) family members have important functions in immunity and oncology, but their roles in cancer immunobiology remain unclear. We aimed to investigate the role of the SLFN family in immune responses against HCC., Methods: Transcriptome analysis was performed in human HCC tissues with or without response to ICIs. A humanized orthotopic HCC mouse model and a co-culture system were constructed, and cytometry by time-of-flight technology was used to explore the function and mechanism of SLFN11 in the immune context of HCC., Results: SLFN11 was significantly up-regulated in tumors that responded to ICIs. Tumor-specific SLFN11 deficiency increased the infiltration of immunosuppressive macrophages and aggravated HCC progression. HCC cells with SLFN11 knockdown promoted macrophage migration and M2-like polarization in a C-C motif chemokine ligand 2-dependent manner, which in turn elevated their own PD-L1 expression by activating the nuclear factor-κB pathway. Mechanistically, SLFN11 suppressed the Notch pathway and C-C motif chemokine ligand 2 transcription by binding competitively with tripartite motif containing 21 to the RNA recognition motif 2 domain of RBM10, thereby inhibiting tripartite motif containing 21-mediated RBM10 degradation to stabilize RBM10 and promote NUMB exon 9 skipping. Pharmacologic antagonism of C-C motif chemokine receptor 2 potentiated the antitumor effect of anti-PD-1 in humanized mice bearing SLFN11 knockdown tumors. ICIs were more effective in patients with HCC with high serum SLFN11 levels., Conclusions: SLFN11 serves as a critical regulator of microenvironmental immune properties and an effective predictive biomarker of ICIs response in HCC. Blockade of C-C motif chemokine ligand 2/C-C motif chemokine receptor 2 signaling sensitized SLFN11 low HCC patients to ICI treatment., (Copyright © 2023 AGA Institute. Published by Elsevier Inc. All rights reserved.)

Background: In this study, we investigated the correlation between odontogenic conditions and the presence of maxillary sinus (MS) abnormalities using cone-beam computed tomography (CBCT) imaging., Methods: The study unit was defined as the ipsilateral MS, maxillary alveolar bone, and posterior teeth. The study included 1,140 study units from 570 patients visualized with CBCT. MS abnormalities, MS septal walls, the anatomical relationship between the MS and the teeth, and missing teeth were recorded. Adjacent odontogenic infections, including periapical lesions, periodontal bone loss, and combined periodontal-endodontic lesions, were documented, and the shortest distance between the infection and the MS floor was measured. The possible correlations between odontogenic conditions and MS abnormalities were analyzed. Whether the anatomical relationship between the MS and the teeth was related to age or sex was analyzed. The chi-squared test, Fisher's exact test, Mann-Whitney U test, Kruskal-Wallis H test, and logistic regression were used for the statistical analyses., Results: MS abnormalities were detected in 57.54% of patients and 42.89% of MSs. Male sex (OR =1.653; P<0.001) and a MS adjacent to teeth with periapical lesions (OR =5.771; P<0.001), periodontal bone loss (OR =2.778; P<0.001), or combined periodontal-endodontic lesions (OR =13.818; P<0.001) increased the probability of MS abnormalities. In MSs with a single infected tooth, male sex (OR =2.413; P=0.045), infected molar (OR =3.431; P=0.008), and a smaller distance between the infection and the MS floor (OR =0.871; P=0.021) increased the probability of MS abnormality. The maxillary root apices of older subjects tended to be farther from the MS (P<0.001)., Conclusions: Adjacent odontogenic infection increased the probability of MS abnormalities. The likelihood of MS abnormality was related to the distance between the infection and the MS, not to the type of infection.

In the era of health big data, with the continuous development of information technology, students' physical health management also relies more on various information technologies. Blockchain, as an emerging technology in recent years, has the characteristics of high efficiency and intelligence. College physical education is an important part of college students' health big data. Unlike cultural classes, physical education with its rich movements and activities, leaves teachers no time to monitor students' real classroom performance. Therefore, we propose a human pose estimation method based on cross-attention-based Transformer multi-scale representation learning to monitor students' class concentration. Firstly, the feature maps with different resolution are obtained by deep convolutional network and these feature maps are transformed into multi-scale visual markers. Secondly, we propose a cross-attention module with the multi-scales. The module reduces the redundancy of key point markers and the number of cross fusion operations through multiple interactions between feature markers with different resolutions and the strategy of moving key points for key point markers. Finally, the cross-attention fusion module extracts feature information of different scales from feature tags to form key tags. We can confirm the performance of the cross-attention module and the fusion module by the experimental results conducting on MSCOCO datasets, which can effectively promote the Transformer encoder to learn the association relationship between key points. Compared with the completive TokenPose method, our method can reduce the computational cost by 11.8% without reducing the performance., Competing Interests: The authors declare there are no competing interests., (©2023 Liu and Li.)

Herein, the synthesis of a new type of catalyst, SBA-M (Schiff complex of different metal types grafted on SBA-15) based on a quaternization reaction, is described. Various amounts of ionic liquid were grafted into the pore channels of SBA-15 using the post-grafting method, which allowed the ionic liquid to be grafted into the pore channels restrictively. Notably, over six cycles, SBA-Mn (0.2) has been shown to maintain its catalytic activity and stability. In addition, a reaction mechanism for the cycloaddition of CO 2 with epoxides based on density-functional theory is proposed. The cycloaddition reaction of CO 2 and epoxides is an efficient way of carbon fixation. It is demonstrated that the metal coordinated with the oxygen atom of the epoxides and that a halogen attacked the carbon of epoxides. Moreover, theoretical calculations and synthesis strategy provide a new approach for CO 2 conversion., (© 2022 Wiley-VCH GmbH.)

Saturable absorber (SA) based harmonic mode-locking (HML) techniques at 2 µm waveband are much less reported than those at 1.5 µm waveband, the maximum repetition rate of the harmonic pulse generated by such techniques at 2 µm waveband is also much lower than those generated at 1.5 µm waveband. In this paper, the 39th harmonic with the repetition rate of 908.6 MHz is realized in a Bi 2 S 3 -based thulium-doped fiber laser. The fundamental mode-locked pulse has a central wavelength of 1954.2 nm and a 3-dB bandwidth of 5.1 nm. The repetition rate is 23.27 MHz and the pulse width is 902 fs. The characteristics of the material and harmonic mode-locked pulse are investigated. To the best of our knowledge, this is the highest and the closest resonance frequency to GHz among the reported SA-based harmonic mode-locked fiber lasers operating at 2 µm waveband.

Background: Targeted sequencing using oncopanels requires comprehensive assessments of accuracy and detection sensitivity to ensure analytical validity. By employing reference materials characterized by the U.S. Food and Drug Administration-led SEquence Quality Control project phase2 (SEQC2) effort, we perform a cross-platform multi-lab evaluation of eight Pan-Cancer panels to assess best practices for oncopanel sequencing., Results: All panels demonstrate high sensitivity across targeted high-confidence coding regions and variant types for the variants previously verified to have variant allele frequency (VAF) in the 5-20% range. Sensitivity is reduced by utilizing VAF thresholds due to inherent variability in VAF measurements. Enforcing a VAF threshold for reporting has a positive impact on reducing false positive calls. Importantly, the false positive rate is found to be significantly higher outside the high-confidence coding regions, resulting in lower reproducibility. Thus, region restriction and VAF thresholds lead to low relative technical variability in estimating promising biomarkers and tumor mutational burden., Conclusion: This comprehensive study provides actionable guidelines for oncopanel sequencing and clear evidence that supports a simplified approach to assess the analytical performance of oncopanels. It will facilitate the rapid implementation, validation, and quality control of oncopanels in clinical use.

Sacral neuromodulation(SNM) procedure has become a new therapy to treat chronic constipation and fecal incontinence. The surgical procedure is easy and safe. It has a small incision compared with traditional surgery and is mainly used in patients whose traditional treatment was unsuccessful. Chronic constipation is one of the most common digestive symptoms. The quality of life in patients with severe constipation has decreased greatly. Although the incidence of fecal incontinence in China is not as high as that of constipation, it also seriously affects the life of the patients, resulting in a decline in the quality of life. Although the mechanism of SNM is uncertain, with more studies conducted, understanding has become more profound, and the curative effect has been recognized. SNM can improve the symptoms and the quality of life. Many studies have reported SNM treatment. Furthermore, some trials on SNM have been conducted. It is used after colorectal resections to promote symptoms of bowel dysfunction. However, few studies reported regarding SNM for constipation and fecal incontinence in China, and knowledge regarding SNM is limited. In this article, we will mainly discuss SNM in the treatment of chronic constipation and fecal incontinence, and its research progress on the mechanism and method, surgical procedure, effectiveness, complications, postoperative contraindications, and the population who need to pay attention, in order to provide reference for the treatment of SNM in China.