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In this study, a series of diblock glycopolymers, poly(6-O-methacryloyl-d-galactopyranose)-b-poly(6-cholesteryloxyhexyl methacrylate) (PMAgala-b-PMAChols), with cholesterol/galactose grafts were prepared through a sequential reversible addition-fragmentation chain transfer (RAFT) polymerization and deprotection process. The glycopolymers could self-assemble into aggregates with various morphologies depending on cholesterol/galactose-containing block weight ratios, as determined by transmission electronic microscopy (TEM) and dynamic laser light scattering (DLS). In addition, the lectin (Ricinus communis agglutinin II, RCA120) recognition and bovine serum albumin (BSA) adsorption of the PMAgala-b-PMAChol aggregates were evaluated. The SK-Hep-1 tumor cell inhibition properties of the PMAgala-b-PMAChol/doxorubicin (DOX) complex aggregates were further examined in vitro. Results indicate that the PMAgala-b-PMAChol aggregates with various morphologies showed different interaction/recognition features with RCA120 and BSA. Spherical aggregates (d ≈ 92 nm) possessed the highest RCA120 recognition ability and lowest BSA protein adsorption. In addition, the DOX-loaded spherical complex aggregates exhibited a better tumor cell inhibition property than those of nanofibrous complex aggregates. The morphology-variable aggregates derived from the amphiphilic glycopolymers may serve as multifunctional biomaterials with biomolecular recognition and drug delivery features.

Machinery can suffer from mechanical vibrations since resonance may be generated from time-varying external excitations under different operation conditions. These detrimental vibrations may significantly influence the device's performance, effectiveness and reliability in operation. In this paper, an innovative, simple and high-efficiency tuned vibration absorber (TVA) consisting of shape memory alloy (SMA) wires, which is referred to a wire-type tuned vibration absorber (WTVA), is proposed to reduce the induced vibration. Experiments are carried out using a six-degree-of-freedom platform which is designed to simulate the frame of precision machinery in practical applications. With the equivalent stiffness of SMA wires adjusted by the controlled electric current, the frequency tunability of WTVA can be achieved. When the natural frequency of WTVA tuned in with the disturbance frequency, the experimental results demonstrate that the efficiency in vibration reduction of the platform is drastically increased even with considerable weight difference between WTVA and the platform. Moreover, the tunable frequency span also increases greatly due to the new design of WTVA and the material characteristics of SMA wires.

3D printing is a new study direction of building method in recent years. The applicability of 3D printing equipment and cement based materials is analyzed, and the influence of 3D printing operation parameters on the printing effect is explored in this paper. Results showed that the appropriate range of 3D printing operation parameters: print height/nozzle diameter is between 0.4 to 0.6, the printing speed 4-8 cm/s with pumpage 9 * 10-2 m 3/ h.

Terpenes are the largest and most diverse class of secondary metabolites in plants and play a very important role in plant adaptation to environment. 3-Hydroxy-3-methylglutaryl coenzyme A reductase (HMGR) is a rate-limiting enzyme in the process of terpene biosynthesis in the cytosol. Previous study found the HMGR genes underwent gene expansion in Gossypium raimondii, but the characteristics and evolution of the HMGR gene family in Gossypium genus are unclear. In this study, genome-wide identification and comparative study of HMGR gene family were carried out in three Gossypium species with genome sequences, i.e., G. raimondii, Gossypium arboreum, and Gossypium hirsutum. In total, nine, nine and 18 HMGR genes were identified in G. raimondii, G. arboreum, and G. hirsutum, respectively. The results indicated that the HMGR genes underwent gene expansion and a unique gene cluster containing four HMGR genes was found in all the three Gossypium species. The phylogenetic analysis suggested that the expansion of HMGR genes had occurred in their common ancestor. There was a pseudogene that had a 10-bp deletion resulting in a frameshift mutation and could not be translated into functional proteins in G. arboreum and the A-subgenome of G. hirsutum. The expression profiles of the two pseudogenes showed that they had tissue-specific expression. Additionally, the expression pattern of the pseudogene in the A-subgenome of G. hirsutum was similar to its paralogous gene in the D-subgenome of G. hirsutum. Our results provide useful information for understanding cytosolic terpene biosynthesis in Gossypium species.

Aiming to solve the problem that unicast or conventional multicast was incapable of make full use of the opening for wireless propagation in orthogonal frequency division multiplexing（OFDM） systems,a resource allocation scheme involving several multicast groups was proposed to maintain proportional fairness among groups.The proposed scheme consisted of subcarrier assignment algorithm and power allocation algorithm.The former was achieved by modi-fying the existing unicast subcarrier assignment algorithm,whilst optimal power allocation on condition that subcarrier assignment was determined was reached by the latter based on theoretically derived non-linear equations.Simulation re-sults show that the proposed scheme can ensure perfect fairness among different multicast groups,and remarkably out-performs conventional multicast in terms of rate-sum capacity.Combined with the low complexity,it is suitable for the application to voice/video multicast in actual wireless systems.

Artificial photosynthesis offers a route to producing clean fuel energy. However, the large thermodynamic requirement for water splitting along with the corresponding sluggish kinetics for the oxygen evolution reaction (OER) limits its current practical application. Here, we offer an alternative approach by replacing the OER with the glycerol oxidation reaction (GOR) for value-added chemicals. By using a Si photoanode, a low GOR onset potential of -0.05 V vs RHE and a photocurrent density of 10 mA/cm2 at 0.5 V vs RHE can be reached. Coupled with a Si nanowire photocathode for the hydrogen evolution reaction (HER), the integrated system yields a high photocurrent density of 6 mA/cm2 with no applied bias under 1 sun illumination and can run for over 4 days under diurnal illumination. The demonstration of the GOR-HER integrated system provides a framework for designing bias-free photoelectrochemical devices at appreciable currents and establishes a facile approach to artificial photosynthesis.

Mice lacking Niemann-Pick C1-Like 1 (NPC1L1) (NPC1L1−/−mice) exhibit a defect in intestinal absorption of cholesterol and phytosterols. However, wild-type (WT) mice do not efficiently absorb and accumulate phytosterols either. Cell-based studies show that NPC1L1 is a much weaker transporter for phytosterols than cholesterol. In this study, we examined the role of NPC1L1 in phytosterol and cholesterol trafficking in mice lacking ATP-binding cassette (ABC) transporters G5 and G8 (G5/G8−/− mice). G5/G8−/− mice develop sitosterolemia, a genetic disorder characterized by the accumulation of phytosterols in blood and tissues. We found that mice lacking ABCG5/G8 and NPC1L1 [triple knockout (TKO) mice] did not accumulate phytosterols in plasma and the liver. TKO mice, like G5/G8−/− mice, still had a defect in hepatobiliary cholesterol secretion, which was consistent with TKO versus NPC1L1−/− mice exhibiting a 52% reduction in fecal cholesterol excretion. Because fractional cholesterol absorption was reduced similarly in NPC1L1−/− and TKO mice, by subtracting fecal cholesterol excretion in TKO mice from NPC1L1−/− mice, we estimated that a 25g NPC1L1−/− mouse may secrete about 4 μmol of cholesterol daily via the G5/G8 pathway. In conclusion, NPC1L1 is essential for phytosterols to enter the body in mice.

Much attention has been paid to the application of low temperature thermal resources, especially for power generation in recent years. Most of the current commercialized thermal (including geothermal) power-generation technologies convert thermal energy to electric energy indirectly, that is, making mechanical work before producing electricity. Technology using a thermoelectric generator (TEG), however, can directly transform thermal energy into electricity through the Seebeck effect. TEG technology has many advantages such as compactness, quietness, and reliability because there are no moving parts. One of the biggest disadvantages of TEGs is the low efficiency from thermal to electric energy. For this reason, we redesigned and modified our previous 1 KW (at a temperature difference of around 120 °C) TEG system. The output power of the system was improved significantly, about 34.6% greater; the instantaneous efficiency of the TEG system could reach about 6.5%. Laboratory experiments have been conducted to measure the output power at different conditions: different connection modes between TEG modules, different mechanical structures, and different temperature differences between hot and cold sides. The TEG apparatus has been tested and the data have been presented. This kind of TEG power system can be applied in many thermal and geothermal sites with low temperature resources, including oil fields where fossil and geothermal energies are coproduced.

Although the equivalent fixture error approach used in variation propagation can directly model the process physics regarding how datum-induced, fixture-induced and machine tool-induced errors generate the same error pattern on part features, the developed equivalent fixture error compensation technique did not consider machining-induced variations. Machining-induced variations are often caused by geometric-thermal effects, cutting force-induced variations, and/or cutting-tool wear, etc. Such machining-induced variations are an important factor that influences the part quality. Without considering machining-induced variations, the application of EFE model for error compensation will be limited. In order to overcome this limitation, this paper extends current equivalent fixture error to include machining-induced variations. This paper shows the benefits of the extended model through a case study.

With the proliferation of advanced persistent threat (APT), APT attack effect evaluation is playing an increasingly important role in cyberspace. As one of the hot issues of network security, the evaluation to its attack effect can quantify the harm caused by APT. Then according to the evaluation results, we can derive specific measures to the network attack. At present, a lot of work has done in the network attack effect evaluation index system. However, a significant barrier to the development of APT attack effect evaluation is that the existing index system is either from the point of view of the network security situation, or for a single attack weapons to customize. In this paper, an evaluation index system is proposed through analysing the features of APT. Through this index system, we can not only quantify APT attack effect, but also visually observe the APT ability from various angles. Then, we use the analytic hierarchy process (AHP) to model the evaluation process and calculate the weight of each indicator. Finally, the Ukrainian Power Outages is taken as an example to validate the proposed index system. The experimental results verify the effectiveness of the index system.

Assembly variations are unavoidable due to parts’ geometrical errors. Statistical variation analysis is an effective method to quantitatively predict product quality in the original design stage. However, traditional methods can’t handle the problem of abnormal distribution of the actual variation variables. Meanwhile, they are underdeveloped in regard to the complex geometrical errors in spatial 3D state. To overcome this problem, firstly, Jacobian-Torsor model is used to build the variation propagation, which is well suited to a complex assembly that contains large numbers of joints and geometric tolerances; secondly, Pearson distribution family is adopted to determine probability distribution pattern and build probability density function. By comparing results of the suggested method to the Monte Carlo method, it is observed that this novel method has the same accuracy, but much higher efficiency. The results also demonstrate that probability distribution types of the parts variations have a significant impact on the final assembling variation.

Idiopathic premature ventricular contractions (PVCs) and ventricular tachycardias (IVTs) originating from the subtricuspid septum and near the His bundle have been reported. However, little is known about the prevalence, distribution, electrocardiographic characteristics and the efficacy of radiofrequency catheter ablation (RFCA) for the ventricular arrhythmias arising from the right ventricular (RV) septum. This study aimed to investigate electrocardiographic characteristics and effects of RFCA for patients with symptomatic PVCs/IVTs, originating from the different portions of the RV septum.Characteristics of body surface electrocardiogram and electrophysiologic recordings were analyzed in 29 patients with symptomatic PVCs/IVTs originating from the RV septum. Among 581 patients with PVCs/IVTs, the incidence of ventricular arrhythmias originating from the RV septum was 5%. Twenty (69%) had PVCs/IVTs from the septal portion of the tricuspid valvular RV region (3 from superoseptum, 15 from midseptum, 2 from inferoseptum), and 9 (31%) from the septal portion of the basal RV (1 from superoseptum, 4 from midseptum, 4 from inferoseptum). There were different characteristics of ECG of PVCs/VT originating from the different portions of the RV septum. Twenty-seven of 29 patients with PVCs/IVTs arising from the RV septum were successfully ablated (93.1% acute success).ECG characteristics of PVCs/VTs originating from the different portions of the RV septum are different, and can help regionalize the origin of these arrhythmias. The septal portion of the tricuspid valvular RV region was the preferential site of origin. RFCA was effective and safe for the PVCs/IVTs arising from the RV septum.

Strategic reading is an important consideration in the L2 reading classroom. This study investigated the effects of a Chinese as a Second Language (CSL) strategies-based reading instruction program, in which participants were explicitly taught ten top-down reading strategies. Thirty-four students enrolled in an advanced-level Chinese class participated in this study and were randomly assigned to treatment and control conditions. This study first examined the influence of the strategies-based instruction on participants' reading comprehension. It then studied participants' perceptions of using reading strategies and of the strategy training program. A series of non-parametric Mann-Whitney U tests showed that the experimental group made significantly greater improvement in overall reading comprehension and in answering global-reference questions. The survey results revealed that although participants generally confirmed the positive effects of reading strategies, they did not have a strong interest in learning reading strategies, or in applying them in L1 or L2 reading contexts.