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An embedded metal-wire nanograting is designed and fabricated for the first time to our knowledge. This artificial material could be used as a broadband polarizing beam splitter to reflect s-polarized light and transmit p-polarized light. An upper-cladding layer of the same material as the gratings is deposited on the ridge of the gratings, whereas the metal wire is deposited in the grating trenches. This embedded structure makes the grating more firm in its applications. High polarization efficiency and low insertion loss with a broad wavelength range (900–1700?nm) and a wide angular tolerance are obtained by optimization of the designed structure.

A new polarization beam splitter/combiner is realized as a one dimensional metal grating structure with periods less than the wavelength; one polarization paralleled to the gratings will be reflected while the orthogonal polarization will be transmitted. We fabricate metal-stripe gratings on glass substrate using E-beam nanolithography technique and reactive ion etching process. A detail investigation of the polarization effect at 1550nm wavelength is carried out with theory analyze and experimental results. The polarization beam splitter has uniform performance with wide variations in the angle of incidence and has low insertion loss, high polarization extinction ratios both the reflected and the transmitted beams.

Brachial plexus injury (BPI) and experimental spinal root avulsion result in loss of motor function in the affected segments. After root avulsion, significant motoneuron function is restored by re-implantation of the avulsed root. How much this functional recovery depends on corticospinal inputs is not known. Here, we studied that question using Celsr3|Emx1 mice, in which the corticospinal tract (CST) is genetically absent. In adult mice, we tore off right C5–C7 motor and sensory roots and re-implanted the right C6 roots. Behavioral studies showed impaired recovery of elbow flexion in Celsr3|Emx1 mice compared to controls. Five months after surgery, a reduced number of small axons, and higher G-ratio of inner to outer diameter of myelin sheaths were observed in mutant versus control mice. At early stages post-surgery, mutant mice displayed lower expression of GAP-43 in spinal cord and of myelin basic protein (MBP) in peripheral nerves than control animals. After five months, mutant animals had atrophy of the right biceps brachii, with less newly formed neuromuscular junctions (NMJs) and reduced peak-to-peak amplitudes in electromyogram (EMG), than controls. However, quite unexpectedly, a higher motoneuron survival rate was found in mutant than in control mice. Thus, following root avulsion/re-implantation, the absence of the CST is probably an important reason to hamper axonal regeneration and remyelination, as well as target re-innervation and formation of new NMJ, resulting in lower functional recovery, while fostering motoneuron survival. These results indicate that manipulation of corticospinal transmission may help improve functional recovery following BPI.

Stem cells have the remarkable potential to develop into many different cell types, essentially without limit to replenish other cells as long as the person or animal is still alive, offering immense hope of curing Alzheimer's disease, repairing damaged spinal cords, treating kidney, liver and lung diseases and making damaged hearts whole. Until recently, scientists primarily worked with two kinds of stem cells from animals and humans: embryonic stem cells and non-embryonic "somatic" or "adult" stem cells. Recent breakthrough make it possible to convert or "reprogram" specialized adult cells to assume a stem stem-like cells with different technologies. The review will briefly discuss the recent progresses in this area.

Torque density of a novel dual-rotor, radial-flux multiphase permanent magnet (PM) machine is enhanced by injecting stator current harmonics without increasing stator yoke thickness. In order to enhance a regular PM machine torque capability by injecting harmonic currents, the no-load air-gap flux density should be designed to be trapezoidal, which increases the flux per pole, and results in a thicker stator yoke. However, this drawback can be avoided in a dual-rotor, radial-flux PM machine. In such a dual-rotor PM machine, the main flux can be designed to go directly from the inner rotor poles, to stator inner and outer teeth, then to the outer rotor poles, or reverse, without through the stator yoke. Therefore, the stator yoke thickness is not affected by air-gap flux at all and can actually be reduced to minimum for mechanical reasons. This paper applies stator current harmonic injection technique to a dual-rotor, radial-flux PM machine. Sizing equations of the multiphase PM machines are deduced and used for initial design and theoretical comparison. The torque performance of the proposed dual-rotor, radial-flux multiphase PM machine with stator harmonic current injection is evaluated using finite element simulation, and compared to a traditional three-phase dual-rotor, radial-flux PM machine. Simulation results validate the feasibility of torque density enhancement. The same technique is proven that it is also suitable in a dual-rotor axial-flux PM machine topology.

In this study, we present a simple yet efficient method to modify polyester electrospun fibrous scaffolds by polysaccharide–protein multilayer formation based on electrostatic interactions. Chitosan (CS) and gelatin (Gel) was selected as the polycation and polyanion, respectively, to build polyelectrolyte multilayers (PEMs) on poly-L-lactide (PLLA) electrospun fibers via layer-by-layer (LbL) self-assembly technique. A positively charged surface was first created via the aminolysis reaction of esters in the PLLA backbone by poly(ethylene imine) (PEI), followed by consequent alternate deposition of Gel and CS. FTIR and XPS measurements confirmed alternative coating of Gel and CS, while SEM observation indicated that the scaffolds maintained the porous and fibrous morphology during PEM development. IMARIS software, which was extensively employed to reconstruct 3D images, was employed to visualize the architecture of neuronal networks and neurite development of single neurons. Our results indicated that the PEM-modification significantly enhanced cell–matrix interactions by improving cell viability and neurite outgrowth. Significantly more branches and longer neurites were obtained on Gel/CS PEM-coated fibrous scaffolds than PLLA fibrous scaffold and those after Gel or CS monolayer modification. Moreover, the component of the outermost layer showed influence on neuron growth in terms of higher cell viability as well as more branches and longer neurites being obtained on Gel-outermost coated fibrous scaffolds than those outermost coated with CS. The current study indicated that the polysaccharide–protein assembly multilayer could be employed to modify the surface of polyester fibers, thus providing a new strategy to fabricate biomimetic scaffolds for nerve tissue engineering.

Despite the obvious clinical interest, our understanding of how developmental mechanisms are redeployed during degeneration and regeneration after brain and spinal cord injuries remains quite rudimentary. In animal models of spinal cord injury, although spontaneous regeneration of descending axons is limited, compensation by intact corticospinal axons, descending tracts from the brainstem, and local intrinsic spinal networks all contribute to the recovery of motor function. Here, we investigated spontaneous motor compensation and plasticity that occur in the absence of corticospinal tract, using Celsr3|Emx1 mice in which the corticospinal tract is completely and specifically absent as a consequence of Celsr3 inactivation in the cortex. Mutant mice had no paresis, but displayed hyperactivity in open-field, and a reduction in skilled movements in food pellet manipulation tests. The number of spinal motoneurons was reduced and their terminal arbors at neuromuscular junctions were atrophic, which was reflected in electromyography deficits. Rubrospinal projections, calretinin-positive propriospinal projections, afferent innervation of motoneurons by calretinin-positive segmental interneurons, and terminal ramifications of monoaminergic projections were significantly increased. Contrary to control animals, mutants also developed a severe and persistent disability of forelimb use following the section of the rubrospinal tract at the C4 spinal level. These observations demonstrate for the first time that the congenital absence of the corticospinal tract induces spontaneous plasticity, both at the level of the motor spinal cord and in descending monoaminergic and rubrospinal projections. Such compensatory mechanisms could be recruited in case of brain or spinal cord lesion or degeneration.

Spinal cord injury (SCI) is a devastating event that results in significant physical disabilities for affected individuals. Apart from local injury within the spinal cord, SCI patients develop a variety of complications characterized by multiple organ dysfunction or failure. These disorders, such as neurogenic pain, depression, lung injury, cardiovascular disease, liver damage, kidney dysfunction, urinary tract infection, and increased susceptibility to pathogen infection, are common in injured patients, hinder functional recovery, and can even be life threatening. Multiple lines of evidence point to pathological connections emanating from the injured spinal cord, post-injury systemic inflammation, and immune suppression as important multifactorial mechanisms underlying post-SCI complications. SCI triggers systemic inflammatory responses marked by increased circulation of immune cells and pro-inflammatory mediators, which result in the infiltration of inflammatory cells into secondary organs and persistence of an inflammatory microenvironment that contributes to organ dysfunction. SCI also induces immune deficiency through immune organ dysfunction, resulting in impaired responsiveness to pathogen infection. In this review, we summarize current evidence demonstrating the relevance of inflammatory conditions and immune suppression in several complications frequently seen following SCI. In addition, we highlight the potential pathways by which inflammatory and immune cues contribute to multiple organ failure and dysfunction and discuss current anti-inflammatory approaches used to alleviate post-SCI complications. A comprehensive review of this literature may provide new insights into therapeutic strategies against complications after SCI by targeting systemic inflammation.