Search

Pistacia atlantica Desf. subsp. mutica is widely distributed in the Zagros Mountains in Iran and its fruits are used as food. In the present study, the morphological and pomological variation of naturally grown male and female genotypes of this species was investigated. Considerable variations were observed among male and also among female genotypes studied based on the characters measured. Leaf length ranged from 11.03 to 17.67 cm in male genotypes and also from 11.98 to 16.14 cm in female genotypes. Furthermore, leaf width varied from 7.32 to 13.08 cm in male genotypes and from 9.18 to 13.31 cm in female genotypes. Inflorescence length ranged between 4.62 and 5.83 cm in male genotypes and between 5.05 and 9.04 cm in female genotypes. Also, 100-nut weight varied from 13.74 to 22.81 g with an average of 16.09, while 100-kernel weight ranged from 2.11 to 3.60 g with an average of 2.65 in female genotypes. Leaf length was significantly and positively correlated with leaf width. Furthermore, 100-kernel weight was positively and significantly correlated with leaf and nut dimensions. Principal component analysis showed that the characters measured accounted for 77.41% of the total variance in male genotypes and also 83.24% of the total variance in female genotypes. The genotypes studied showed high variation based on the characters recorded. The wide adaptation of P. atlantica Desf. subsp. mutica highlights its potential as a genetic resource for pistachio breeding for resistance to abiotic and biotic stresses such as drought. The current findings may be useful to select conservation strategies and genetic improvement of the species studied.

Objetivo: Describir la variedad de nochebuena Ximena en base a directrices de la UPOV y evaluar su comportamiento con un productor cooperante en el estado de Morelos.Diseño/metodología/aproximación: La variedad se generó mediante los métodos genotécnicos de hibridación, selección recurrente y uso de la técnica de injerto. La descripción varietal se realizó en base al documento de la Unión para la Protección de las Obtenciones Vegetales (UPOV). El diseño experimental utilizado fue un completamente al azar con 10 repeticiones. Se registraron caracteres vegetativos y de bráctea de la planta. La información se estudió mediante análisis de varianza y prueba de comparación de medias Tukey (P? 0.05).Resultados: Ximena presenta porte alto, de amplitud grande con ramificación intermedia. La hoja de forma oval, larga y ancha con peciolo de longitud media. Brácteas de forma elíptica, color rojo, sin torsión y rugosidad entre los nervios. La anchura de la cima es media, glándulas del ciatio de color amarillo de tamaño medio sin alguna deformación. La respuesta de Ximena fue estadísticamente similar a los dos testigos en diámetro del tallo, número de entrenudos, ancho de hoja, longitud de peciolo de hoja y bráctea. En cambio, fue superior estadísticamente en longitud de bráctea, amplitud de dosel de bráctea y diámetro de ciatio.Limitaciones del estudio/implicaciones: La variedad se mantiene como planta madre, a partir de la cual se van a establecer cultivos comerciales. Por lo que, requiere adecuada nutrición y condiciones que no permitan su diferenciación floral.Hallazgos/conclusiones: El comportamiento y arquetipo de Ximena fue comparable a la de variedades comerciales, posee características estéticas que demanda el mercado, puede ser competitiva y se considera candidata para diversificar la oferta de plantas de nochebuena en la temporada de navidad.

Introduction: Light stress is an important factor limiting the biomass yield while combining forage production with crops or forestry. Guinea grass is a widely adapted perennial fodder grass. The species exhibits high degree of variation for morphology, adaptation and biomass yield. Objective: Since there is a need in identifying shade adapted forage grasses for the expanding area under agroforestry/silvipastures, the present investigation took the task of understanding how the morphologically distinct genotypes of guinea grass respond under different shaded intensities. Methods: In the present study, forty-four genotypes related with the shade response were studied in varying shading conditions (pure sunlight, 25, 50 and 75 % shade) created artificially. Results: Based on green and dry matter yields ranking, the genotype IG 01-98 performed the best followed by genotypes IG 01-92, IG 97-5, IG 97-6 and IG 01-89 in decreasing order. Particularly, IG 01-93 was identified as the best performing under 50 % shading conditions. On the other hand, most of the top ranking genotypes performed well both under open and up to 50 % of shade. Morphologically, these genotypes were taller and possessed longer and broader leaves. Under shaded conditions (over 50 %), leaf length and width showed an increasing trend compared to open conditions. Also, chlorophyll content increased with shading intensity. Conclusions: Most of the genotypes collected from the southern Indian humid tropical environment with early flowering nature were tolerant to shade. Differential genotypic response was observed for biomass yield and yield attributes under shade. The study established appreciable variability for shade tolerance among genotypes. Resumen Introducción: El estrés leve es un factor importante que limita el rendimiento de la biomasa al tiempo que combina laproducción forrajera con los cultivos o la silvicultura. El zacate guinea es una planta de forraje perenne ampliamente adaptada. La especie presenta un alto grado de variación en su morfología, adaptación y rendimiento de biomasa. Objetivo: Como existe la necesidad de la identificación de pastos forrajeros adaptados a la sombra para el área de expansión bajo agroforestería /silvicultura, la presente investigación se realizó para entender cómo los genotipos morfológicamente distintos al zacate guinea responden a diferentes intensidades de sombra. Métodos: Se estudiaron 44 genotipos y su respuesta a los niveles de sombra: luz solar pura y 25, 50 y 75 % de sombra (creados artificialmente). Resultados: Basado en la clasificación de rendimientos demateria verde y seca, el genotipo IG 01-98 fue el mejor, seguido de los genotipos IG 01-92, IG 97-5, IG 97-6 e IG 01-89 en orden decreciente. Por su parte, el IG 01-93 fue identificado como el de mayor rendimiento bajo el 50 % de condición de sombra. La mayoría de los genotipos de primer nivel tuvieron un buen desempeño, tanto bajo sombra abierta como al 50 %. Morfológicamente, estos genotipos fueron más altos y poseían hojas más largas y más anchas. En condiciones de sombra, de más del 50 %, la longitud y ancho de la hoja mostraron una tendencia creciente en comparación con la condición abierta. El contenido de clorofila aumentó con la intensidad de la sombra. Conclusiones: La mayoría de los genotipos recolectados al sur de la India en un ambiente tropical húmedo y tienen una floración temprana, fueron tolerantes a la sombra. Se observó una respuesta genotípica diferencial para los atributos de rendimiento y rendimiento de biomasa bajo sombra. El estudio mostró la existencia de una variabilidad apreciable para la tolerancia a la sombra entre los genotipos.

PREMISE The nondestructive measurement of leaf area is important for expediting data acquisition in the field. The Montgomery equation (ME) assumes that leaf area (A) is a proportional function of the product of leaf length (L) and width (W), i.e., A = cLW, where c is called the Montgomery parameter. The ME has been successfully applied to calculate the surface area of many broad-leaved species with simple leaf shapes. However, whether this equation is valid for more complex leaf shapes has not been verified. METHODS Leaf A, L, and W were measured directly for each of 5601 leaves of 15 vine species, and ME and three other models were used to fit the data. All four models were compared based on their root mean square errors (RMSEs) to determine whether ME provided the best fit. RESULTS The ME was a reliable method for estimating the A of all 15 species. In addition, the numerical values of 13 of the 15 values of c fell within a previously predicted numerical range (i.e., between 1/2 and π/4). The data show that the numerical values of c are largely affected by the value of W/L, the concavity of the leaf base, and the number of lobes on the lamina. CONCLUSIONS The Montgomery parameter can reflect the influence of leaf shape on leaf-area calculations and can serve as an important tool for nondestructive measurements of leaf area for many broad-leaved species and for the investigation of leaf morphology.

Morphological integrations are unknown to forestry seeds. Understanding if seed measurements could predict its future seedlings features is a central question in seedling production.Hymenaea courbarilis an important species in this context and to the applied forestry. Thus, this study aimed to understand how some seedling features, could be related to the seed weight, and be affected by the population origin. The measurements consisted of seedling collar diameter, weight, protophilus area, central and lateral vein. Seed weight consistently varied between the populations in the study. Both populations had higher weight ranges than mentioned in the literature. There was no strong evidence that greater seed weight requires lesser time to germinate, conflicting with previous information. All the measurements presented enough shreds of evidence to be considered different when comparing the populations, except for the protophilus area and lateral vein length. All the studied measurements presented low correlation indexes to seed weight, except for seedling collar diameter, and seedling weight, which presented a moderate correlation. Protophilus elongation pattern was strongly associated with the leaf width when compared to midvein.

Mumtazah HM, Supriyono, Widyastuti Y, Yunus A. 2020. The diversity of leaves and asiaticoside content on three accessions of Centella asiatica with the addition of chicken manure fertilizer. Biodiversitas 21: 1035-1040. Centella asiatica (L.) commonly known as an essential medicinal herb. This plant has high diversity, especially in leaf morphology. The main objective of this research was to determine the morphology of leaves and the effect of chicken manure dosage on the increase of asiaticoside content. The leaves of three accessions characterized, and then C. asiatica planted. The research design used a completely randomized design (CRD) factorial, with the first factor is accession (C) with 3 levels (C1: accession 1, C2: accession 2, C3: accession 3). The second factor was chicken manure with 4 levels dosage (P1: 0 ton ha-1, P2: 10 tons ha-1, P3: 15 tons ha-1 and P4: 20 tons ha-1). Asiaticoside analysis used the TLC method with chloroform: methanol: water (65:25:4) as mobile phase. The result from this research showed that the diversity in leaf morphology of three accessions is in leaf size and petiole length. Accession 3 has the widest leaf size (7.3 cm) and the longest petiole (9.8 cm), accession 2 with a leaf width of 5.8 cm and petiole length in 8.5 cm, while the accession 1 has the least leaf width (5.3 cm) and the length of the petiole is the shortest with 7.3 cm. Observation results of asiaticoside showed that the highest content of asiaticoside produced by accession 3 (0.19%), then accession 1 with a content of 0.15%, while accession 2 showed the lowest asiaticoside content (0.13%). The treatment of chicken manure addition significantly increase asiaticoside content in accessions 1 and 3, but in accession 2 it was not significantly increased. The highest content of asiaticoside (0.34%) were in accession 3 with the addition of chicken manure 20 tons ha-1 (P4).

portuguesOs objetivos deste trabalho foram verificar se ha variabilidade genetica e avaliar as relacoes lineares entre a arquitetura de planta e a produtividade de graos de milho. Foram conduzidos tres experimentos no delineamento blocos completos ao acaso. Em 51 cultivares, foram avaliados caracteres relacionados ao numero de folhas, as alturas de planta e de espiga, ao ângulo foliar, ao comprimento da folha, a largura da folha, a area foliar e a produtividade de graos, totalizando 22 caracteres. Foram realizadas analises de variância individuais, verificados os pressupostos de normalidade dos erros e homogeneidade de variâncias residuais e agrupadas as medias de cultivares pelo teste de Scott-Knott. Foi determinada a matriz de correlacao fenotipica entre os 22 caracteres das 51 cultivares. Ha variabilidade genetica entre as cultivares em relacao ao numero de folhas, alturas de planta e de espiga, ângulo foliar, comprimento da folha, largura da folha, area foliar e produtividade de graos. Folhas localizadas proximas a espiga apresentam menor ângulo foliar e maiores comprimento da folha, largura da folha e area foliar. O ângulo foliar aumenta gradativamente em direcao as folhas da extremidade inferior e superior da planta. O comprimento da folha, a largura da folha e a area foliar diminuem gradativamente em direcao as folhas da extremidade inferior e superior da planta. Cultivares com maior numero de folhas e maior area foliar estao associadas a plantas com maior produtividade de graos EnglishThe objectives of this study were to analyze whether there is genetic variability and assess the linear relationships between plant architecture and maize grain yield. Three experiments were carried out in a complete randomized block design. A group of 51 cultivars was assessed in relation to 22 traits: number of leaves, plant height, ear height, leaf angle, leaf length, leaf width, leaf area, and grain yield. Individual analyses of variance were performed, the assumptions of normality of errors and homogeneity of residual variances were tested, and means were grouped by the Scott-Knott test. The phenotypic correlation matrix was constructed using the 22 traits of the 51 cultivars. Results showed that there is genetic variability among cultivars for number of leaves, plant height, ear height, leaf angle, leaf length, leaf width, leaf area, and grain yield. Leaves close to the ear have smaller leaf angle and larger length, width, and area of the leaves. Leaf angle gradually increases towards the lower and upper ends of the plant. Length, width, and area gradually decreases in leaves towards the lower and upper ends of the plant. Cultivars with higher number of leaves and larger leaf area are associated with higher grain yield

The present study used fractal analysis for soybean leaves characterization (Glycine max (L.) Merr), Caro and Onix genotypes. The box-counting method was used to find the fractal dimensions of leaf shape in the two genotypes. The fractal dimensions were D = 1.627 at the Caro genotype, and respectively D = 1.509 at the Onix genotype. In the case of Caro genotype, correlation analysis showed average correlations between fractal dimension (D) and leaf width (W), r = 0.728, respectively between D and scan leaf area (SLA), r = 0.778 and low intensity correlations between D and perimeter of the leaves (Per), r = 0.624, respectively between D and the leaf length (L), r = 0.484. In the case of Onix genotype, high correlations were found between D and SLA (r = 0.862), between D and W (r = 0.812); average correlations between D and Per (r = 0.766), and respectively low correlations between D and L (r = 0.685). Based on D values, SLA prediction was possible under conditions of R2 = 0.631, p << 0.001 for Caro genotype, and R2 = 0.812, p << 0.001 respectively for Onix genotype.

EnglishThe compost from the waste of pharmaceutical industries, such as guava leaves (GL) and neem seeds (NS) is used in organic agriculture. Curcuma zanthorrhiza RoxB. is a widely recognized herbal medicine that grows natively in Indonesia. Curcuminoids and xanthorrhizol are the primary bioactive components of C. zanthorrhiza. In this study, we aimed to evaluate the impact of GL and NS compost on the growth and bioactive yields of C. zanthorrhiza. Treatments consisted of cow manure, GL compost, NS compost, GL+NS compost, or a no compost control, at 2 and 4 kg per plant. Results demonstrated that the NS and GL+NS compost applied with 4 kg per plant had increased fresh rhizome yield compared with the other treatments. Composted NS at 2 kg per plant increased the plant height and pseudo stem diameter traits compared with the control treatment. The compost application of GL+NS at 2 and 4 kg per plant significantly increased the leaf length and number of shoots. All treatments showed unchanged the leaf width and number of leaves. The compost application of GL and NS (2 kg per plant) showed higher production of curcuminoidsthan the control. The compost consisting of GL (2 kg per plant), NS (4 kg per plant), and GL+NS also increased the production of xanthorrhizol compared with the control treatment. Results illustrated the practical application of GL and NS composts from industrial pharmaceutical extraction waste for the organic farming cultivation of C. zanthorrhiza portuguesO composto dos residuos das industrias farmaceuticas, como folhas de goiaba (GL) e sementes de nim (NS), e usado na agricultura orgânica. Curcuma zanthorrhiza RoxB. e um medicamento fitoterapico amplamente reconhecido que cresce de forma nativa na Indonesia. Os curcuminoides e o xanthorrizol sao os principais componentes bioativos de C. zanthorrhiza. Neste trabalho, objetivou-se avaliar o impacto do composto GL e NS no crescimento e produtividade bioativa de C. zanthorrhiza. Os tratamentos consistiram em esterco de vaca, composto GL, composto NS, composto GL + NS ou controle sem composto, em 2 e 4 kg por planta. Os resultados demonstraram que o composto NS e GL + NS aplicado com 4 kg por planta aumentou a producao de rizoma fresco, em comparacao com os outros tratamentos. A NS compostada a 2 kg por planta aumentou as caracteristicas de altura da planta e diâmetro do pseudoestemico em comparacao com o tratamento controle. A aplicacao de composto de GL + NS em 2 e 4 kg por planta aumentou significativamente o comprimento das folhas e o numero de brotacoes. Todos os tratamentos apresentaram alteracao na largura e numero de folhas. A aplicacao de composto de GL e NS (2 kg por planta) apresentou maior producao de curcuminoide do que o controle. Os compostos constituidos por GL (2 kg por planta), NS (4 kg por planta) e GL + NS tambem aumentaram a producao de xanthorrizol em comparacao com o tratamento controle. Os resultados ilustraram a aplicacao pratica de compostos GL e NS de residuos de extracao farmaceutica industrial para o cultivo orgânico de C. zanthorrhiza

Key message A highly significant and positive scaling relationship between bamboo leaf dry mass and leaf surface area was observed; leaf shape (here, represented by the quotient of leaf width and length) had a significant influence on the scaling exponent of leaf dry mass vs. area. Context The scaling of leaf dry mass vs. leaf area is important for understanding how plants effectively intercept sunlight and invest carbon to do so. However, comparatively few, if any, studies have focused on whether leaf shape influences this scaling relationship. Aims In order to explore the effects of leaf shape on the scaling relationship between leaf dry mass and area, we examined 101 species, varieties, forms, and cultivars of bamboo growing in China and identified the relationship between the scaling exponent of leaf dry mass vs. area and leaf shape. This taxon was used because its leaf shape is conserved across species and, therefore, easily quantified. Methods Ten thousand and forty-five leaves from 101 bamboo species, varieties, forms, and cultivars growing in China were collected, and leaf dry mass, the quotient of leaf width and length, leaf area, and leaf dry mass per unit area were measured. The effect of leaf shape that can be easily quantified using the quotient of leaf width and length on the relevant and ecologically important scaling exponents was explored using this data base. Results Leaf dry mass and area differed significantly across bamboo genera, and even within the same genus. However, a statistically robust log-log linear and positive scaling relationship was observed for mass and area with a 1.115 scaling exponent (95% CI = 1.107, 1.122; r2 = 0.907). Leaf shape had a significant influence on the numerical values of the scaling exponent of leaf dry mass vs. area. When the median of the quotient of leaf width and length was below 0.125, the numerical value of the scaling exponent increased with increasing quotient of leaf width and length. When the median of the quotient of leaf width and length was above 0.125, the scaling exponent numerically decreased toward 1.0. Conclusion We show, for the first time, that a significant relationship exists between leaf shape and the numerical values of scaling exponents governing the scaling of leaf dry mass with respect to leaf area. In addition, we show that with the quotient of leaf width and length increasing mean LMA increases, which implies a negative correlation between mean LMA and the estimated exponent of leaf dry mass vs. area for the grouped data based on the sorted quotients of leaf width and length.

Although plant organ shapes are defined by spatio-temporal variations of directional tissue expansion, this is a little characterized aspect of organ growth regulation. Although it is well known that the plant hormone gibberellin increases the leaf length/with ratio, its effects on cell expansion in the growing leaf are largely unknown. To understand how variations in rate and anisotropy of growth establish the typical monocotelydonous leaf shape, we studied the leaf growth zone of maize (Zea mays) with a kinematic analysis of cell expansion in the three directions of growth: proximo-distal, medio-lateral, and dorso-ventral. To determine the effect of gibberellin, we compared a gibberellin-deficientdwarf3mutant and the overproducing UBI:GA20OX-1 line with their wild types. We found that, as expected, longitudinal growth was dominant throughout the growth zone. The highest degree of anisotropy occurred in the division zone, where relative growth rates in width and thickness were almost zero. Growth anisotropy was smaller in the elongation zone, due to higher lateral and dorso-ventral growth rates. Growth in all directions stopped at the same position. Gibberellin increased the size of the growth zone and the degree of growth anisotropy by stimulating longitudinal growth rates. Inversely, the duration of expansion was negatively affected, so that mature cell length was unaffected, while width and height of cells were reduced. Our study provides a detailed insight in the dynamics of growth anisotropy in the maize leaf and demonstrates that gibberellin specifically stimulates longitudinal growth rates throughout the growth zone.

The study was conducted to determine the impacts of environmental variations on yield and yield related properties of tobacco. The experimental layout was in the randomized blocks with three replicates in four different locations (Erbaa-Evciler, Erbaa-Karayaka, Gümüşhacıköy, Bafra) using 21 lines and four standard varieties that stand out with their different characteristics. The variation in plant height, number of leaves, leaf width, leaf length and leaf yield of genotypes were investigated at different ecologies. All the parameters investigated of the genotypes have been significantly affected by changing environmental conditions. Plant height ranged from 49.33 cm to 177.32 cm, the number of leaves from 21.83 to 47.10 per plant, leaf width from 6.83 to 16.31 cm, leaf length from 13.01 to 28.93 cm, and yield ranged from 79.17 kg-1 to 238.98 kg da-1. The results for the performances of genotypes in all environments showed that 5 lines (ERB-11, ERB-14, ERB-16, ERB-21, ERB-35) for plant height, 6 lines (ERB-6, ERB-7, ERB-11, ERB-14, ERB-16, ERB-35) for the number of leaves, 10 lines (ERB-9, ERB-16, ERB-17, ERB-18, ERB-19, ERB-21, ERB-25, ERB-26, ERB-27, ERB-30) for leaf width, 1 line (ERB-16) in for leaf length and 13 lines (ERB-6, ERB-7, ERB-9, ERB-13, ERB-16, ERB-18, ERB-19, ERB-21, ERB-25, ERB-27, ERB-30, ERB-35, ERB-38) for yield come to the fore.

The characteristic traits of maize (Zea mays L.) leaves affect light interception and photosynthesis. Measurement or estimation of individual leaf area has been described using discontinuous equations or bell-shaped functions. However, new maize hybrids show different canopy architecture, such as leaf angle in modern maize which is more upright and ear leaf and adjacent leaves which are longer than older hybrids. The original equations and their parameters, which have been used for older maize hybrids and grown at low plant densities, will not accurately represent modern hybrids. Therefore, the aim of this paper was to develop a new empirical equation that captures vertical leaf distribution. To characterize the vertical leaf profile, we conducted a field experiment in Jilin province, Northeast China from 2015 to 2018. Our new equation for the vertical distribution of leaf profile describes leaf length, width or leaf area as a function of leaf rank, using parameters for the maximum value for leaf length, width or area, the leaf rank at which the maximum value is obtained, and the width of the curve. It thus involves one parameter less than the previously used equations. By analysing the characteristics of this new equation, we identified four key leaf ranks (4, 8, 14 and 20) for which leaf parameter values need to be quantified in order to have a good estimation of leaf length, width and area. Together, the method of leaf area estimation proposed here adds versatility for use in modern maize hybrids and simplifies the field measurements by using the four key leaf ranks to estimate vertical leaf distribution in maize canopy instead of all leaf ranks.

This study aims to determine to determine the effect of land spinach plant growth due to the provision of liquid organic fertilizer palm sugar and to determine the right concentration of palm sugar palm POC to the growth of land spinach plants. This experiment used a single factor randomized block design consisting of 4 replications of 4 treatments. The best experimental results on plant height and number of leaves, leaf length and leaf width aged 14 and 24 HST were influenced by the use of palm juice 40 ml / L water with a height of 19.59 cm and 32.13 cm number of leaves 6.34 and 12.25 strands, leaf length 13.71 and 15.75 while leaf widths are 1.74 and 3.48 cm.

The aim of this study was to evaluate oat genotypes for grain yield and yield components in both 2014-2015 and 2015-2016 growing seasons using GGE biplot analysis. Experiments were laid out according to lattice design. Biomass at harvest, grain yield, number of grain per panicle, weight of grain per panicle, flag leaf width, flag leaf height, plant height, stem diameter, thousand kernel weight, time of panicle emergence, harvest index, panicle length, and spikelet per panicle were evaluated for 56 oat genotypes. GGE biplot graphics of the first and second years explained 54.4 and 55% of total variation, respectively. Grain yield, number of grains per panicle, and weight of grain per panicle were closely and positively associated in both growing seasons. Recently registered cultivar Sari and lines 26, 29, and 30 were found as promising genotypes for Canakkale conditions. Traits of genotype at different growing seasons can be visually studied using different genotype-by-trait (GT) biplots.

This study was conducted to investigate the efficacy of freshwater alga, Chlorella fusca on the improvement of growth and qualities in organic spinach and Chinese chives farm. The average height of Chinese chives treated with the chlorella was 3.7 cm smaller than that of the untreated. The leaf width and fresh weight of Chinese chives treated with the chlorella was 0.5 mm wider and 30.3 g heavier than that of the untreated. The commercialization and yield of Chinese chives treated with the chlorella was 11.9% and 18.3%, respectively higher than that of the untreated. Also, the disease severity of gray mold disease of Chinese chives treated with the chlorella was reduced by more than 24.2% when compared with the untreated. The thickness and number of spinach leaves treated with chlorella was 27.9% and 41.8%, respectively higher than that of the untreated. The fresh weight and yield of the spinach treated with the chlorella was 63.6% and 31.5%, respectively higher than that of the untreated. Moreover, the mineral content of K, Ca, Mg, P, Fe, and Mn were recorded higher in the spinach treated with chlorella compared with that of untreated. The results indicated that the freshwater alga, Chlorella fusca is efficient and economical biostimulant in improving plant growth and quality of Chinese chives and spinach in organic farm.

Dendrobium sylvanum orchid is categorized as lowland habitus orchid and able to survive wihtout any shade. Orchid cultivation takes a very long time so it needs faster cultivation and producing large quantities. Therefore, it is necessary to do orchid culture by using tissue culture processes and then given gamma ray irradiation to bring up new characters in the orchid .The purpose of this research was to discover the impact of gamma ray irradiation in vitro to the diversity of D. sylvanum. The doses of gamma ray used in this research were 0 Gy, 15 Gy, 30 Gy, 45 Gy, and 60 Gy. The results obtained indicated that mutation mostly occurred on the treatment with 15 Gy (4.4 cm of plant height, 2.67 of leaves, 2.36 cm of leaf length, 0.49 cm of leaf width, and 5.33 of root strands) compared to the other doses and the control plants. The new finding in this research is the gamma ray dose that can optimally stimulate the mutation in D. sylvanum. This information is useful to generate the new variety in orchid cultivation in Indonesia. This research provides innovation in orchid cultivation and new variety that is possible to arise after the mutation.

Purpose To quantify the contribution of penumbra in the improvement of healthy tissue sparing at reduced source‐to‐axis distance (SAD) for simple spherical target and different prescription isodoses (PI). Method A TPS‐independent method was used to estimate three‐dimensional (3D) dose distribution for stereotactic treatment of spherical targets of 0.5 cm radius based on single beam two‐dimensional (2D) film dosimetry measurements. 1 cm target constitutes the worst case for the conformation with standard Multi‐Leaf Collimator (MLC) with 0.5 cm leaf width. The measured 2D transverse dose cross‐sections and the profiles in leaf and jaw directions were used to calculate radial dose distribution from isotropic beam arrangement, for both quadratic and circular beam openings, respectively. The results were compared for standard (100 cm) and reduced SAD 70 and 55 cm for different PI. Results For practical reduction of SAD using quadratic openings, the improvement of healthy tissue sparing (HTS) at distances up to 3 times the PTV radius was at least 6%–12%; gradient indices (GI) were reduced by 3–39% for PI between 40% and 90%. Except for PI of 80% and 90%, quadratic apertures at SAD 70 cm improved the HTS by up to 20% compared to circular openings at 100 cm or were at least equivalent; GI were 3%–33% lower for reduced SAD in the PI range 40%–70%. For PI = 80% and 90% the results depend on the circular collimator model. Conclusion Stereotactic treatments of spherical targets delivered at reduced SAD of 70 or 55 cm using MLC spare healthy tissue around the target at least as good as treatments at SAD 100 cm using circular collimators. The steeper beam penumbra at reduced SAD seems to be as important as perfect target conformity. The authors argue therefore that the beam penumbra width should be addressed in the stereotactic studies.

This study was conducted to examine the influence of Light-Emitting Diode (LED) light quality in urban agricultural plant factories on the growth and development of Seolhyang strawberry daughter plants in order to improve the efficiency of daughter plant growth and urban agriculture. LED light quality by demonstrated that above-ground growth and development were greatest for daughter plant 2. Daughter plant 1 showed the next highest growth and development, followed by daughter plant 3. Among the different qualities of LED light, the stem was thickest and growth rate of leaves was highest for R + B III (LED quality: red 660 nm + blue 450 nm/photosynthetic photon flux density (PPFD): 241–243 μmol·m-2·s-1) and lowest for R (red 660 nm/115–117 μ mol·m-2·s-1). Plant height, leaf width, petiole length, and the leaf growth rate were highest for W (white fluorescent lamp/241–243 μ mol·m-2·s-1) and lowest for R + B Ⅰ (red 660 nm + blue 450 nm/80–82 μ㏖·m-2·s-1). For above-ground growth and development, as the plants surpassed the seedling age, mixed light (red + blue), rather than monochromatic light (red or blue), and higher PPFD values tended to increase development. Regarding the quality of the LED light, daughter plant 2 showed the highest chlorophyll content, followed by daughter plant 1, and daughter plant 3 showed the least chlorophyll content. When the wavelength was monochromatic, chlorophyll content increased, compared to that when PPFD values were increased. Mixed light vitality was highest in daughter plant 2, followed by 1, and 3, showed increased photosynthesis when PPFD values were high with mixed light, in contrast to the results observed for chlorophyll content.

The present experiment was carried out to determine mutagenic sensitivity on Jasmine (Jasminum spp.). In this experiment, terminal cuttings of Jasminum grandiflorum cv. White Pitchi and semi hardwood cuttings of J. nitidum (clone Acc.Jn-1 selected at TNAU) and J. multiflorum cv. Arka Arpan were treated with ten different concentrations of EMS ranging from 5 mM to 50 mM. . The results revealed gradual and significant reduction in survival of cuttings, shoot length, number of leaves, leaf length and leaf width with increase in dosage of EMS. The probit curve analysis based on survival percentage and growth rate of treated cuttings revealed LD50 dosage of EMS to be 37.15 mM, 40.7 mM and 42.66 mM for White Pitchi, Arka Arpan and ACC Jn-1 respectively.

Authors. 2018. Morphometric characteristics of two seagrass species (Enhalus acoroides and Cymodocea rotundata) in four small islands in North Maluku, Indonesia. Biodiversitas 19: 2035-2043. Seagrass has an important ecological role as a protector of coastlines and small islands. It is known to be capable of forming phenotypic plasticity through morphometric variation as a response to its environmental conditions. This study aimed to determine the distribution and morphological differences between populations of two important seagrass species (Enhalus acoroides and Cymodocea rotundata) in the waters around four small islands in North Maluku. The study was conducted at two stations in each of Ternate Island and Maitara Islands, three stations at Tidore Island and one station at Hiri Island. A line transect method was used to collect seagrass samples. Morphometric characteristics included leaf sheath length, leaf length, leaf width, rhizome diameter, root length, root diameter, and internode length were measured. Discriminant analysis was performed to describe morphometric characteristics distinctive of the four islands. Leaf sheath length, leaf length, rhizome diameter, and root length of E. Acoroides differed significantly among the populations from the various islands except for leaf width. Meanwhile, leaf sheath length, leaf width, rhizome diameter, root length, and internode length differed significantly in C. rotundata among the populations from different islands except for leaf length.

This systematic determination of morphological and phytochemical data was conducted with the purpose of conserving and identifying the phylogenetic relationship among the Vanilla species of the Totonacapan region in Mexico to increase awareness of the genetic biodiversity. Samples of Vanilla planifolia, V. planifolia cv. “oreja de burro”, V. pompona, V. insignis, and V. inodora, are distributed across 19 municipalities of the State of Veracruz and 19 municipalities of the State of Puebla. Morphological data parameters were determined in situ and included leaf length, leaf width, leaf thickness, stem diameter, stem thickness, node distance, stem texture degree, flower colour intensity, and fruit length. Similarly, alkaloids, tannins, saponins, phenols, flavonoids, and terpenes were determined by specifically phytochemical tests and quantified by thin layer chromatography. Both, morphological and phytochemical data parameters, were successfully used in assembling dendrograms by using the Euclidian distance method and by principal component analysis. Keywords: Dendogram, Morphological data, Phylogenetic relationships, Phytochemical data, Principal component analysis, Vanilla sp

Polianthes tuberosa L. is propagated using tubers which are pseudostems that change shape and function as food storage reserves. The Research was conducted at Lampung State Polytechnic in May 2017 to March 2018. This research used the Perfect Safe Group Design with two treatment factors. The first factor consists of large diameter (1,6 – 2 cm), medium (1,4 – 1,5 cm) and small (1,3 – 1 cm). The second factor consists of Atonik growth regulating substances which are 0 (without treatment), 1 ml.l-1and 3 ml.l-1 . Data were analyzed using variance fingerprints, if they contributed significantly (significant), then the data was continued by using the Duncan Multiple Range Test (DMRT) at a significant level of 5%. The results of this study indicate that the size of bulbs (1,6 cm – 2 cm) produces plant length (38,9 cm), number of leaves (16,1 strands), leaf width (1,9 cm) and number of tillers (9, 8 shoots) the fastest and longevity of panicle plants (24,9 MST) and age of flower blooms (28,3 MST) which are faster. Medium tuber size (1,4 cm – 1,5 cm) and small (1 cm – 1,3 cm) available the longest flower stalk length (79,8 cm and 86,6 cm).

To develop a controlled-release fertilizer (CRF) suitable for nutrient absorption characteristics of Phalaenopsis, four kinds of new controlled-release fertilizer (NCRF 1–4) with different dissolution rates were developed and studied to determine the concentration and amount suitable for growth of Phalaenopsis. To make NCRF, new acryl-based polymers were developed and used as fertilizer coating solutions. In addition, a fluidized bed coater for coating fertilizer was developed and used in this study. To test the growth of Phalaenopsis, 10-month-old Phalaenopsis seedlings were planted in plastic pots (diameter 10 cm) filled with 100% Sphagnum moss and cultivated for approximately 100 days from May 29, 2015, to September 11, 2015. NCRF 1, NCRF 2, and Osmocote, an imported fertilizer, consistently exhibited release patterns of fertilizer nutrients in a directly proportional form; however, NCRF 3 and NCRF 4 displayed a sigmoid-like tendency of fertilizer nutrient release with a slower initial dissolution rate. Furthermore, leaf length, leaf width, fresh weigh, and root weight of Phalaenopsis were the highest when growing in 1.5 g/pot of NCRF 3 fertilizer, and the pH and electrical conductivity (EC) of the soil were stable at this concentration of NCRF 3. Based on our results, we suggest that 1.5 g/pot of NCRF 3 fertilizer is the ideal concentration and fertilizer for growing Phalaenopsis.

A field experiment was conducted during winter season of 2015-16 to study 'Studies Effect of Planting Dates and Varieties on Growth and Quality on Gladiolus (Gladiolus hybridus Hort.) under sub-humid zone of Rajasthan' having 15 treatment combinations of five Varieties (V1- African Star, V2- Hunting Song, V3- Legend, V4- Pusa Srijana and V5- Snow Princess) and three planting dates (D1- 10th October, D2- 25th October and D3- 9th November). The treatment V2D1 (Hunting Song + 10th October planting) recorded the maximum plant height (116.10 cm), highest number of leaves per plant (8.77), main stem diameter (1.50 cm), number of florets per spike (15.8), spike length (91.80 cm), rachis length (57.90 cm), floret diameter (10.04 cm), vase life of spike (13 days), While the maximum leaf length (64.20 cm), leaf width (4.96 cm) in (V5D3) and maximum spike girth (0.91cm) was found in (V1D2). The growth and flowering quality was found superior in compare to other planting dates.

The purpose of this research is to know the relationship between vegetative growth with sweet corn produced in experimental garden of Faculty of Agriculture Unilak. The environmental design used was a complete randomized design with 20 replications. Data analysis using correlation analysis. The parameters observed were plant height, leaf number, leaf length, leaf width, weight of cob weighing, cob weight without cornhusk, length of cob weighing, length of cob without cornhusk. The results showed that plant height, leaf number, and leaf width were significantly correlated with unbalanced cob weight, and correlated significantly with the length of cobs weighing and length of cobs without cornhusk. Plant height, leaf number, and leaf width did not give a significant correlation to the weight of cobs weighing. Weight of cobs weighing with unobstructed weight of cobs without real correlated weight, and long cobs without weight with weightlessness showed no real correlation.

Leaf area (LA) is a valuable key for plant physiological studies, therefore accurate and simple models for LA determination are important for many experimental comparisons. Field experiment was conducted at the Teaching and Research Farm of the Federal University of Agriculture, Abeokuta (07o15’N, 03o 25’E) in the forest-savannah transition zone of South West Nigeria in 2013 to estimate leaf area (LA) of peppermint (Mentha piperita L.) using functions between plant LA and fresh weight (FW), dry weights (DW) and leaf dimensions (width-W and length-L) to identify appropriate functions for use in models estimating leaf area of peppermint. Leaf samples were randomly selected from the lower, middle and upper parts of the plant at 30, 60, 90 and 120 days after transplanting (DAT). Leaf length, width, L2, W2, product of these dimensions and leaf fresh and dry weights of 150 leaf samples were assessed and compared with actual leaf area measured by graph tracing method, to test their accuracy and reliability using Y = a + bX model. There was a highly significant correlation (r = 0.6 to 0.9) between actual leaf area and the corresponding leaf length, width, L2, W2, product of these dimensions and leaf fresh and dry weights. Regression analyses of LA versus FW, DW, L, W, L2, W2 and the products of these dimension revealed several models that could be used for estimating the area of individual peppermint leaf. Among the models, one based on length dimension (LA = a + bL) r = 0.9, R2 = 0.96, RMSE = 0.03 was the most accurate. To validate this model, actual leaf area of 60 leaf samples obtained by graph tracing method was compared with leaf area estimated by the model at 30, 60, 90 and 120 DAS in another trial in 2014 wet season. The leaf area estimated by the models strongly agreed with the measured value of leaf area as evident from high value of R2 (0.99) and low RMSE (0.03). The validation of the models indicates that model (LA = a + bL) was accurate and reliable to determine the leaf area of peppermint and therefore would be very useful for field workers dealing with large samples.

Ramadiana S, Hapsoro D, Yusnita Y.2018. Morphological variation among fifteen superior robusta coffee clones in Lampung Province, Indonesia. Biodiversitas 19: 1475-1481. This study aimed to characterize morphological variation among fifteen superior robusta coffee clones in Lampung Province. The fifteen clones consisted of four clones released by the Indonesian Coffee and Cocoa Research Institute (ICCRI), i.e. ‘BP 409’,‘BP936’, ‘BP939’, ‘SA 237’, together with eleven superior coffee clones selected by farmers from Tanggamus District (‘Tugino’, ‘Wanto’, ‘Biyadi’, ‘Komari’, ‘Wardi’, ‘Wariso’) and from West Lampung District (‘Tugu Kuning’, ‘Tugu Hijau', ‘Tugu Biru', ‘Tugu Sari', ‘Lengkong'). Fifteen qualitative and seven quantitative morphological characters were evaluated in a randomized complete block design with three replicates for each clone,each replication consisted of two plants. From each plant, four samples were taken from four sides of the plant (north, south, west, and east). It was found that while some morphological characters exhibited negligible variation among clones, there were significant differences between clones for other characters. The characters that did not differ significantly between clones were: shapes of leaf base and leaf tip; petiole color; leafvenation pattern; fruit disk shape; ripe-fruit color; and stipule shape. The characters that varied between clones were shape of leaf lamina (elliptical vs. lanceolate); fruit shape (round vs. oval); and shape of leaf margins. Morphological variation was also observed in some quantitative characters: canopy diameter; tree height; stem diameter; leaf length; leaf width; petiole length; stipule length; and number of primary branches

It is believed that black mulberry (Morus nigra) is a healthier fruit among mulberry species due to its high bioactive content. In the present study, the morphological and chemical variabilities of this species were investigated. There were statistical differences in terms of the morphological and chemical traits between the genotypes. Out of 37 morphological and seven chemical characters, 20 morphological and five chemical traits showed a coefficient of variation (CV) greater than 20.00%, indicating a high variability between the genotypes for these traits. The fruit weight varied between 1.79 and 5.69 g with an average of 3.85 g. The highest TSS content was 24.80%, while the lowest TSS was 11.20%, with an average of 18.42. The content of total anthocyanins was found between 57.62 and 475.65 mg CGE 100 g−1 FrWe. Total phenols ranged from 232.27 to 1777.73 mg GAE 100 g−1 FrWe. The fruit weight showed positive correlations with tree growth vigor, leaf length and leaf width. In addition, fruit weight was positively correlated with fruit length and fruit width. Furthermore, TSS was positively correlated with phenolic content and flavonoid content. The results obtained from the hierarchical grouping analysis separated genotypes into three and two main groups based on morphological and chemical characters, respectively. The present study increased knowledge about the phenotypic variation and phytochemical properties of black mulberry fruits and can be useful for producers, breeders, and processors.

Oats are widely used in cold regions as forage and grain crop, with early maturing oat varieties having long planting history. More attention is paid to variety maturity, including both naked and hulled types, for production in cold regions. Despite wide planting of early maturing oat in the cold regions in China, no systematic evaluation on yield and quality of the oat varieties in these regions has been conducted. Qinghai and Inner Mongolia are typically cold regions in China due to high elevation and high latitude respectively, and are representative areas for experimentation on growing oats in cold regions. In this study, we tested production and quality of early and late maturing oat varieties at five locations in Qinghai Province and one location in Inner Mongolia Autonomous Regions. We measured several growth, yield and quality parameters of four early maturing and three late maturing oat varieties in the former locations and six early maturing and one late maturing oat varieties at the latter locations. Parameters measured included leaf length, leaf width, panicle length, dry forage yield, seed yield crude protein and others. Attention was primarily paid to oats maturity due to the emphasis on forage and crop productivity without cons derma for to what the varieties were naked and hulled. The results showed that, in the regions of Qinghai Province with high altitude, forage yield and quality parameters of the early maturing varieties were not significantly different from those of the late maturing varieties. However, seed yield of the early maturing varieties (3702 kg/hm2) was significantly higher than that of the late maturing varieties (2759 kg/hm2) ( P

We aimed to propose a reliable and accurate model using non-destructive measurements of leaf length (L) and/or width (W) for estimating leaf area (LA) of Surinam cherry ( Eugenia uniflora L.). For model construction, 560 leaves were randomly sampled from different levels of the tree canopies and encompassed the full spectrum of measurable leaf sizes. Power models better fit E. uniflora leaf area than linear models; but, among of then, the best fit were made when product of the L and W (LW) were used. To validate these models, independent data set of 156 leaves were used. Thus, we developed a single power model (Yi = β0 xβ1) [LA = 0.685 (LW)0.989; standard errors: β0 = 0.014, β1 = 0.005; R2 a = 0.997] with high precision and accuracy, random dispersal pattern of residuals and unbiased. A simpler linear model [LA = 0.094 + (LW * 0.655); standard errors: β0 = 0.025, β 1 = 0.001; R2 a = 0.998] also described here to estimate leaf area of E. uniflora, which are as good as the first. The simplicity of the latter model may be relevant in field studies, as it does not demand high precision or expensive instruments.

The present study was aimed to determine genetic diversity of Coffea arabica landraces based on morphological characteristics at seedling stage in Guji zones. Three kebeles and twenty-five farmers’ coffee landraces were selected based on availability of Coffea arabica landraces from each district by the guidance of Developmental Agency in the kebele. Coffea arabica seeds were collected and planted at kercha nursery sub-site in plastic pots arranged on randomized block design. Seedling characteristics were collected from one year old randomly selected four coffee seedlings from each plot. The results showed that there were significant variations (p

To accelerate the mulberry breeding program and examine the genome size and ploidy levels of 27 mulberry accessions of promising lines in Taiwan, nuclei suspensions were prepared from unfolded young leaves and analyzed by a flow cytometer. The genome size of each accession was estimated by referring to the standard genome size of chicken erythrocyte nuclei (CEN, 2.5 pg). Our results indicated that 25 among 27 investigated accessions in M. bombycis, M. alba, M. latifolia, M. australis, M. formosnensis, and M. atropurpurea are diploids with genome sizes ranging from 0.61 to 0.71 pg. ‘Elongated mulberry No. 1’ (M. laevigata) with a genome size of 1.06 pg is a triploid. Two populations were identified in the accession ‘67C001’ (M. australis Poir.) with a genome size of either 0.63 pg or 0.98 pg. Due to their similarity in appearance, we suspected that they are probably from the same origin, thus warranting further investigations. Several important vegetative characters of diploids and triploids have been estimated, including leaf length, leaf width, petiole length, petiole width, leaf thickness, and internode length, to document their correlation using the unpaired t-test. Subsequently, the statistical relationship between diploid and triploid species on some of these important characters, including leaf width, petiole width and internode length, was determined through binary regression analysis. We suggested that the internode length could provide a beneficial index to distinguish individuals with different ploidy levels in the field. Results of this study provide useful information to clarify the taxonomy of Morus and improve breeding programs aiming to advance horticulture and sericulture.

Research conducted an experiment using a completely randomized design environment with four replications, and the design of treatment used is six varieties of sweet corn. Analysis of data using polynomial regression, the parameters measured were plant height, leaf number, leaf length, leaf width, and sugar beans, followed by analyzing the relationship between the growth of plants with a sugar content of sweet corn kernels. The results showed that leaf width gives a weak relationship to the sugar content of sweet corn seed, and leaf length, number of leaves and plant height had a close relationship to the sugar content of sweet corn kernels. Keywords: varieties, sweet corn, correlation, sugar content

Pohpohan (Pilea trinervia Wight.) is potential indigenous vegetable to be developed as a commercial vegetables. The objectives of this research were to observe similarity and production potential of pohpohan landraces from several areas in West Java. Result based on cluster analysis at nine similarity scale, thirteen exploration pohpohan landraces were grouped into three clusters. Clusters I consisted of Warung Loa, Tugu Selatan, Palasari, Langensari, Kayu Ambon, Lebak Muncang, Situsari, Sukalilah and Lebaksiuh. Clusters II consisted of Curug Rendeng, Argalingga and Linggarjati and clusters III was Bobojong. Result from further test using Duncan Multiple Range Test (DMRT), Warung Loa is a leading landrace because showed the best result on plant height, primary branches number, leaf width, yield per plot and plant productivity. Keywords: Cluster analysis, exploration, indigenous vegetable

Nowadays people are increasingly concerned about the importance of product quality. The use of fertilizers derived from organic materials is believed to bring more benefits to agricultural products where products become healthier, environmentally friendly and can reduce the negative impact of chemicals that are harmful to humans and the environment. Veg mole is expected in addition to its local microorganisms also Nitrogen element content can increase the growth and production of mustard plants.The purpose of this study is to determine the effect and to get a good dose of MOL vegetable waste on the growth and production of mustard plants.The experiment was conducted experimentally using non factorial completely Randomized Design (RAL) consisting of 5 treatment levels and 4 replications to obtain 20 experimental units. Each plot consists of 16 plants and 4 plants as sample. The level of treatment is as follows: Mo: Without giving MOL of vegetable waste, M1: Giving MOL vegetable waste 75 cc / l water, M2: Giving of MOL vegetable waste 150 cc / l water, M3: Giving MOL vegetable waste 225 cc / l water , M4: Giving MOL vegetable waste 300 cc / l waterThe result of this research can be concluded that vegetable MOL has no significant effect on all observed parameters, ie plant height (cm), number of leaf (strands), leaf length (cm), leaf width (cm), wet weight (g) consumption (g). Keywords: vegetable waste, mustard greens

Variability, correlation and path analysis of 24 yield and quality characters were assessed in turmeric using 15 genotypes. Wide genetic variability was observed among the genotypes for most of the characters studied. High GCV and PCV was observed for 17 characters except for plant height, leaf length, leaf width, primary rhizome length, secondary rhizome girth, diameter of inner core of primary rhizome and diameter of primary rhizome. High heritability coupled with high genetic advance as per cent of mean was observed for 23 characters except secondary rhizome girth. Simple correlation analysis revealed that fresh rhizome yield per plant showed positive and significant correlation with collar girth, length of mother rhizome, girth of mother rhizome, weight of mother rhizome, number of primary rhizome, weight of primary rhizome and number of secondary rhizome. From path analysis, it is understood that fresh rhizome yield exhibited high direct positive effect on girth of mother rhizome, weight of mother rhizome, weight of primary rhizome, number of secondary rhizome and curcumin content.

Twenty four accessions of Plantago ovata were grown to study existing variability, correlation and cause and effect relationships between yield and its component traits with their possible view in future improvement programmes. Analysis of variances displayed highly significant differences among accessions for all the traits. High heritability (>75%) coupled with the high genetic advance (>30%) were observed for the traits viz., leaf width, number of spikes/plant, biological yield/plant and seed yield/plant suggesting predominance of additive gene action. Seed yield/plant exhibited highly significant and positive correlation with biological yield/plant followed by number of spikes/plant, effective tillers/plant, harvest index, seed weight/spike, spike weight and husk recovery both at genotypic and phenotypic level. Biological yield/plant, harvest index, spike weight, effective tillers/plant and husk recovery exhibited the highest positive and significant direct effect on seed yield/plant. Critical analysis of results obtained from character association and path analysis indicated that the traits viz., biological yield/plant, harvest index, spike weight, effective tillers/plant and husk recovery were of prime concern as they possessed high positive association and direct effects on seed yield/plant. These traits are more reliable for selection for yield improvement programmes in Plantago ovata.

Forty diverse genotypes of cluster bean [ Cyamopsis tetragonoloba (L.) Taub.] were assessed in a field experiment entitled performance study of cluster bean germplasms under Lucknow condition in Randomized Block Design with three replications. The experiment was conducted at the Horticulture Research Farm of the Department of Horticulture, Babasaheb Bhimrao Ambedkar University, Lucknow- 226025 (U.P.) during Rabi season of 2016. The performance studies of 40 diverse genotypes of cluster bean and considerable variation was recorded for all the characters. The observations were recorded on 19 characters viz ., plant height (cm), plant width (cm), leaf length, leaf width (cm), number of cluster, length of pod (cm), width of pods (cm), breath of pods (cm), number of pods, number of leaves, width of leaves, seed per pod, weight of plant without leaves (g), weight of green leaves (g), weight of dry leaves (g), leaf length/petiole ratio (cm), stem width, crude protein (%), ash (%) and dry matter (%).

Background and purpose: Several studies have investigated multi leaf collimator (MLC) leaf design. We performed a systematic review and meta-analysis of those studies to compare the impact of MLC leaf width used for different radiotherapy techniques. Materials and methods: We decided to focus on 2.5, 3.0 and 5.0 mm leaf width MLCs as it appeared to be the most contentious area from literature. We adopted Cochrane and PRISMA guidelines and computed the association between MLC leaf width and conformity index (CI) across the selected studies as pooled mean difference (PMD) with 95% confidence interval. Results: A total of 43 papers were selected from the literature search, of which ten compare MLC leaf width of 2.5 mm or 3.0 mm (MLC2.5 mm) versus 5.0 mm (MLC5 mm) in terms of CI. There was a slight, but significant, difference between MLC2.5 mm and MLC5 mm in favor of the former (mean difference −0.036; 95% confidence interval: −0.068 to −0.005). A subgroup analysis was performed by comparing techniques (intensity modulated radiation therapy vs conformal). In the intensity modulated radiation therapy (IMRT) subgroup, the difference between MLC2.5 mm and MLC5 mm appeared to be negligible (mean difference: −0.006; 95% confidence interval: −0.013 to 0.001) and not significantly different from zero. In the subgroup of studies which used conformal techniques, there was a significant difference between MLC2.5 mm and MLC5 mm (mean difference: −0.054; 95% confidence interval: −0.096 to −0.012). Conclusions: Introduction of IMRT produced comparable target coverage (CI) between 2.5 or 5.0 mm leaf width MLCs. Keywords: Multi leaf collimators, Treatment planning system, Intensity modulated radiation therapy, Systematic review, Meta-analysis

Key message QTL controlling flag leaf length, flag leaf width, flag leaf area and flag leaf angle were mapped in wheat. Abstract This study aimed to advance our understanding of the genetic mechanisms underlying morphological traits of the flag leaves of wheat (Triticum aestivum L.). A recombinant inbred line (RIL) population derived from ND3331 and the Tibetan semi-wild wheat Zang1817 was used to identify quantitative trait loci (QTLs) controlling flag leaf length (FLL), flag leaf width (FLW), flag leaf area (FLA), and flag leaf angle (FLANG). Using an available simple sequence repeat genetic linkage map, 23 putative QTLs for FLL, FLW, FLA, and FLANG were detected on chromosomes 1B, 2B, 3A, 3D, 4B, 5A, 6B, 7B, and 7D. Individual QTL explained 4.3–68.52% of the phenotypic variance in different environments. Four QTLs for FLL, two for FLW, four for FLA, and five for FLANG were detected in at least two environments. Positive alleles of 17 QTLs for flag leaf-related traits originated from ND3331 and 6 originated from Zang1817. QTLs with pleiotropic effects or multiple linked QTL were also identified on chromosomes 1B, 4B, and 5A; these are potential target regions for fine-mapping and marker-assisted selection in wheat breeding programs. Electronic supplementary material The online version of this article (10.1007/s00122-017-3040-z) contains supplementary material, which is available to authorized users.

This work was carried out at Ghazala Experimental Farm, Fac. Agric., Zagazig Univ., Egypt, during the two successive winter seasons of 2015/2016 and 2016/2017, to estimates the genetic parameters for Balady cabbage, using North Carolina Mating Design II. Ten selected plants were used for this study (five as males and other five as females) from Balady cabbage population. Results of this study showed insignificant mean squares for male × female interaction for all the studied traits, except that for stem diameter, leaf width, wrapper leaves weight per plant and head height, which were highly significant. Estimations of additive variance (σ2A) were higher than dominance variance (σ2D) for stem diameter, leaf width, head height, head diameter and head weight per plant (yield), indicate the important of additive gene action in the inheritance of these traits. Nevertheless, σ2D was higher than σ2A for stem height from wrapper leaves to head, leaf length, and wrapper leaf number and weight per plant, so that the dominance was played very important role in the inheritance of these traits. The heritability in broad-sense ranged from -4.30% for stem height up to wrapper leaves to 83.80% for leaf width. While, narrow-sense of heritability ranged from -2.10% for stem height up to wrapper leaves to 97.35% for head height.

El presente estudio evaluó el efecto de diferentes concentraciones y tiempo de exposición de colchicina, sobre la morfología foliar y los estomas de Kalanchoe daigremontiana. Inicialmente, se recolectaron y se sometieron las plántulas de K. daigremontiana a concentraciones de 0,025% y 0,1% (p/v) de colchicina y a dos tiempos de exposición (24 y 48 horas). Posteriormente, se realizaron estudios morfológicos como la altura de la planta (AP), anchura de la hoja (AH), número de hojas (NH), longitud foliar (LH), espesor de hoja (EH) y volumen foliar (VH) cada 15 días, durante 16 semanas después de la siembra. Luego, se caracterizaron los estomas, teniendo en cuenta el ancho, el largo, índice estomático y el número de cloroplasto por estoma. Se encontró un incremento significativo en la morfología foliar y estomática, en los tratamientos con colchicina de 0,025% a 24 y 48 horas. Lo que demuestra que el uso de la colchicina logra un mayor crecimiento en poco tiempo y aumento de la biomasa en la planta medicinal K. daigremontiana.

This study was aimed at establishing allometric models for estimating LA (Leaf Area) of eight Coffea arabica genotypes in Mana district of Jimma Zone Oromia Regional State, South Western Ethiopia (7°46'N, 36°0'E). Many Methodologies and instruments have been devised to facilitate measurement of leaf area. However, these methods are destructive, laborious and expensive. For modeling leaf area, leaf width, leaf length and leaf area of 1200 leaves (50 leaves for each genotype) was measured for model calibration and the respective measurements on 960 leaves were used for model validation. Linear measurement was taken from leaves and branch diameters of eight genotypes of C. arabica, cultivated in field following a randomized complete blocks design at three altitudes (High, Medium and Low) were evaluated to identify best option for input in the models, and to validate the method to estimate the leaf area. Linear and non-linear models were tested for their accuracy to predict leaf area of the eight C. arabica genotypes. The use of linear model resulted in high accuracy for all of the eight C. arabica genotypes. No significant effect of growing altitude and genotype was obtained among the slopes of the models. Therefore, one single model was fitted to the combined data of all genotypes at all altitudes (LA = 0.6434LW). Comparison between observed and predicted leaf area was made using this model in another independent dataset, conducted for model validation, exhibited a high degree of correlation (r = 0.98 - 0.99, P < 0. 01). The over or under estimation of the leaf area using this model ranges between 0.02% to 1.7% and this model is adequate to estimate the leaf area for the eight C. arabica genotypes. Hence, this model can be proposed to be reliably used and with this developed model, researchers can estimate the leaf area of newly released eight genotypes of C. arabica at different altitudes accurately.

We aimed to propose a reliable and accurate model using non-destructive measurements of leaf length (L) and/or width (W) for estimating leaf area (LA) of three genotypes of purging nut ( Jatropha curcas L.), i.e. Astrea and Barranca , both Colombian and a Mexican genotype. For model construction, ~500 healthy leaves were collected from at least 20 healthy plants naturally grown at each locations, and encompassed the full spectrum of measurable leaf sizes. Equations with L and/or W were generated; however, the best fit were made when product between L and W (LW). To validate these models, independent data set of 450 leaves were used. Thus, we developed a single model ( Y i = β 0 * LW β1 ) with high precision and accuracy, random dispersion pattern of residuals and unbiased. In spite of this, a single equation, joining all the studied genotypes, was developed [LA = 0.822 * (LW) 0.983 ; standard errors: β 0 = 0.020, β 1 = 0.004; R 2 a = 0.976], which greatly simplifies the process of measuring the leaf area of the genotypes, a fact that should be highlighted.

Present study was undertaken to evaluate different morphological traits of rice advance lines. The experiment was laid out in randomized complete block design (RCBD) with ten rice advance lines replicated three times at National Tea and High Value Crops Research Institute (NTHRI) Shinkiari, Mansehra, Pakistan. Three plants per plot were selected randomly to record the important agro-morphological data viz. plant height, panicle length, number of tillers per plant, branches per panicle, flag leaf length, flag leaf width, flag leaf area, grain length, grain width and length/breadth (L/B) ratio were measured. All the recorded data was analyzed through SPSS 16.0 and Statistix 8.1. The results revealed that the vegetative growth attributes i.e. plant height was significantly increased in FH10-2 (152.7 cm), No. of tillers in FH4-1 (4.3), No. of branches in JP-6 (24.33), panicle length in Line 2(24) (49.6 cm), flag leaf length in FH10-2 (50.7 cm), flag leaf width in Super NPT-3 (4.3 cm) and flag leaf area in FH4-1 (143.6 cm), respectively. Furthermore, yield contributing attributes like grain length (8.5 mm) and Length/Breadth ratio (4.7) were significantly increased in FH4-1 and grain breadth in Super NPT-3 (2.8 mm). Among ten advance lines of rice, nine were recorded with slender grain shape while four advance lines were recorded with extra long slender and long slender grain size each.

CO2 capture and storage (CCS) is an important technical strategy to reduce global CO2 emission from heavy industries. However, the risk of CO2 leakage in CCS-related projects cannot be ignored and it is crucial to identify and monitor CO2 leakage in CCS project areas in a timely and effective manner. So here we investigate the biological characteristics (plant height, leaf length, leaf width and SPAD value) and leaf spectral characteristics of maize under soil CO2 concentration of 10, 30, and 50%, with normal soil condition as control (CK). By analysing the original spectrum and first derivative spectrum of maize leaves, spectral parameters relatively sensitive to soil CO2 stress were extracted. Further, eight composite spectral characteristic indexes that are expected to identify CO2 leakages were constructed and analyzed. Besides, the normalized pigment chlorophyll index (NPCI) and modified chlorophyll absorption in reflectance index (MCARI) were also investigated for the indication role in CO2 leakage. The results showed that maize morphological traits had a significant hysteresis effect for the indication of CO2 leakage; the SPAD value was difficult to indicate the lower concentration of soil CO2 early. But it was found that three spectral characteristic indexes REP-BEP, RGP/RRV and RGP-RRV/RGP+RRV can effectively identify CO2 leakage, and three spectral characteristic indexes (RVP-GPP, REP-BEP and MCARI) can be used for quantitative inversion of soil CO2 concentration. Especially, REP-BEP index could both indicate CO2 leakage and quantitatively inverse soil CO2 concentration. In sum, the spectral characteristic indexes of maize leaves can be used as effective indicators to quickly and effectively identify CO2 leakage and quantitatively invert the CO2 concentration in soil.

Sugar palm is a commodity that has the potential to be developed. Sugar palm is developed to produce various products and raw materials for derivative products. Currently, there is a lot of morphological diversity of sugar palm plants found in the field. Presently, identification of various types of sugar palm morphologically in Banten Province is still limited. Sugar palm in Banten Province is found in various locations, such as in Lebak. This study aimed to determine the characteristics of Aren seedlings originating from Lebak Regency. The hypothesis in this study is that there are several characteristics of palm seedlings originating from Lebak Regency. This research was conducted in August 2019 - November 2019 located in Green House, Cinanggung Village, Serang City, Banten Province. This study uses a descriptive method, through a quantitative approach with data analysis and interpretation, and conclusion. The observation parameters in this study were: Plant Height (cm); Number of leaves per plant (strands); Leaf width (cm); Leaf length (cm); stem diameter (cm); Leaf color. The results showed that Aren from Lebak Regency aged 1 year have an average plant height of 28.1 cm, a pinnate leaf shape and a thick like a palm leaf, with an average number of 1 leaves, an average leaf length of 16.4 cm, and an average leaf width of 6.8 cm, with dark green leaf color. The stem diameter has a strength correlation with plant growth level like as plant height, the higher the plant. The greater the stem diameter obtained in this study is 2.19 cm.