Search

Determination of chilling and heat requirements is respected in the selection of the suitable cultivars for producers to avoid damages caused by an inadequate winter chilling in a certain area. It also play an important role within a breeding program, when selecting parents to get late or early flowering cultivars. The present study aimed to estimate the chilling and heat requirements of breaking bud dormancy for flowering and vegetative growth in three Iranian almond cultivars in an orchard located in the suburbs of Garmdarreh (32.45°N; 50.91°E; altitude 1960 m). The three almond cultivars had different flowering times, i.e. early: ʻMohebʼ, medium: ʻMamaieʼ and late: ʻRabiʼ. To provide adequate chilling, fourteen-year-old trees were exposed to low temperature treatments (4 ± 1 °C) to simulate 0–1500 h of chilling at 250 h intervals. The cultivars varied in their chilling and heat requirements in terms of chilling hours and growing degree hours, GDH, to achieve bud break for flowering, i.e. 'Moheb' (250 h and 2132 GDH), 'Mamaie' (500 h and 2460 GDH) and 'Rabi' (500 h and 2624 GDH). To achieve bud break for leaf growth, the late-leafing cultivar ('Rabi') required 750 chilling hours and a heat requirement of 3608 GDH. The earlier-leafing cultivars ('Moheb' and 'Mamaie') had lower heat requirements for leaf unfolding. Although 'Rabi' and 'Mamaie' had similar chilling requirements of 500 h, 'Rabi' required almost a week (168 h) of warm weather after chilling to achieve bud break. This implies that spring frost is rarely a problem for 'Rabi' in Iran. 'Moheb' was not suitable because of its low chilling and heat requirements, hence its inability to escape early spring frosts and late winter cold. [ABSTRACT FROM AUTHOR]

This study investigated the mechanisms responsible for the antiproliferative activities of three Ruellia tuberosa (RT) leaf extracts against MCF-7 cells after liquid-liquid partitioning. Among these extracts, methanol leaf extract exhibited the greatest inhibition of cell growth compared to water and hexane extracts. Results from JC-1 staining and apoptosis assay demonstrated that these leaf extracts induced apoptosis via caspase or mitochondrial independent manner as only substantial apoptotic cells with no obvious loss of mitochondrial membrane potential were found. However, the decrease in the BCL-2/BAX ratio is due to upregulated BAX expression, implying cells undergoing apoptosis. The expression of caspase 9 was unaltered except for cells treated with hexane leaf extract. Additionally, perturbation of cell cycle progression mainly at G1 or S phases was noticeable in treated cells and concentration-dependent. The distinct effect of different leaf extracts on the cell cycle was also reflected by the dysregulation of cyclins (A2 or E1) and cyclin-dependent kinase (CDK6). These findings have unveiled the therapeutical value of RT leaf extracts in breast cancer. [ABSTRACT FROM AUTHOR]

Sufficient food must be available to the people in order to feed the population of Pakistan. The dairy business, which provides milk, meat, and other goods for human use, is crucial in this regard. Similar to this, it is imperative to offer animals enough nutrient-rich diet so they can produce their optimum amount specifically during the fodder scarcity time. Multicut Kharif fodder varieties that can supply cuttings throughout the aforementioned lean season must be developed. At the Fodder Research Sub Station of the AARI in Faisalabad, an endeavor was made to create a Napier variety that could be used for two different things i.e. (nutritious fodder and multicut in nature). A selection pedigree method was used in this case based on the local “Faisalabad Napier” parentage. The goal was to create an advanced line with the highest green fodder yield and the greatest capacity for resprouting. Advanced line “FNG-1” has a multicut nature and produces more green fodder. “FNG-1” is the multicut (6- 7 cuttings) tall growing advance line in nature, cost of production is much lower as compared to conventional Kharif fodder crops. It is developed locally and highly adopted to the agro-ecological conditions of the province. Due to perennial and multicut in nature its green fodder yield potential is 6 times higher than traditional kharif fodders (Sorghum, millet, jantar etc). Due to its maximum re sprouting tendency it can provide green fodder during the lean period. Due to broader greener leaves and tall growth, FNG-1 can give 15 percent higher GFY than Local accession (Faisalabad Napier). In this way, it can serve the dual purpose as nutritious green fodder to the livestock and as muticut. [ABSTRACT FROM AUTHOR]

Copyright of Industrial Minerals & Processing / Huagong Kuangwu yu Jiagong is the property of Industrial Minerals & Processing Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Background: The Dendrobium officinale leaves (DOL) is an underutilized by-product with a large biomass, which have been shown to exhibit immunomodulatory and antioxidant functions. The purpose of this research was to investigate the effects of DOL on broiler growth performance, antioxidant status, immune function, and gut health. Methods: One hundred and ninety-two 1-day-old chicks were selected and divided into 4 groups at random, 6 replicates for each group and 8 in each. Chicks were given a basal diet supplemented with different amounts of DOL: 0% (control group, NC), 1% (LD), 5% (MD), or 10% (HD). During the feeding trial (70 days), broiler body weight, feed intake, and residual feeding were recorded. On d 70, 12 broilers from each group were sampled for serum antioxidant and immune indexes measurement, intestinal morphological analysis, as well as 16S rRNA sequencing of cecal contents and short-chain fatty acid (SCFA) determination. Results: In comparison to the NC group, the LD group had greater final body weight and average daily gain, and a lower feed conversion ratio (p < 0.05, d 1 to 70). However, in MD group, no significant change of growth performance occurred (p > 0.05). Furthermore, DOL supplementation significantly improved the levels of serum total antioxidant capacity, glutathione peroxidase, superoxide dismutase, and catalase, but reduced the level of malondialdehyde (p < 0.05). Higher serum immunoglobulin A (IgA) content and lower cytokine interleukin-2 (IL-2) and IL-6 contents were observed in DOL-fed broilers than in control chickens (p <0.05). Compared to the NC group, duodenal villus height (VH) and villus height-to-crypt depth (VH:CD) ratio were considerably higher in three DOL supplementation groups (p < 0.05). Further, 16S rRNA sequencing analysis revealed that DOL increased the diversity and the relative abundance of cecal bacteria, particularly helpful microbes like Faecalibacterium, Lactobacillus, and Oscillospira, which improved the production of SCFA in cecal content. According to Spearman correlation analysis, the increased butyric acid and acetic acid concentrations were positively related to serum antioxidant enzyme activities (T-AOC and GSH-Px) and immunoglobulin M (IgM) level (p < 0.05). Conclusion: Overall, the current study demonstrated that supplementing the dies with DOL in appropriate doses could enhance growth performance, antioxidant capacity, and immune response, as well as gut health by promoting intestinal integrity and modulating the cecal microbiota in broilers. Our research may serve as a preliminary foundation for the future development and application of DOL as feed additive in broiler chicken diets. [ABSTRACT FROM AUTHOR]

The globose scale (GS) (Sphaerolecanium prunastri Boyer de Fonscolombe) is a sucking insect that feeds on saps of wild apricot tree (Prunus armeniaca L.) in the Xinjiang Uygur Autonomous Region of northwestern China. It feeds on branches of wild apricot leading to poor growth, leaf yellowing and leaf drop, and sometimes mortality. Since the initial infestation in 2018, all the main valleys of wild apricot trees have been affected, but there is little research on the host's physiological response to GS infestation. We measured the differences in growth between infested and non-infested wild apricots. The results showed that the diameter of shoot branches, the fresh weight, dry weight, length, width, area, and chlorophyll content of leaves, and the longitudinal diameter of fruit, were all significantly less for infested wild apricot trees than for un-infested wild apricot. The branch length of shoots, cross diameter, and weight of fruit also decreased, although the differences were not significant. Overall, GS infestation significantly reduced the growth of wild apricot trees. [ABSTRACT FROM AUTHOR]

Secondary metabolites in Cyclocarya paliurus (Batalin) Iljinsk. leaves are beneficial for human health. The synthesis and accumulation of secondary metabolites form a complex process that is influenced by the trade-off between primary and secondary metabolism and by the biosynthetic pathways themselves. In this study, we explored the relationship between secondary metabolite accumulation and the activity of metabolic networks in leaves of C. paliurus. Leaves at three different growth stages were subjected to transcriptomic and non-targeted metabolomic analyses. The results revealed that nitrogen assimilation increased and carbon assimilation decreased as leaves matured, and the patterns of secondary metabolite accumulation and gene expression differed among the leaves at different growth stages. Mature green leaves had higher nitrogen assimilation and lower carbon assimilation, which were correlated with variations in secondary metabolite accumulation. As a major source of carbon and nitrogen, glutamine accumulated in the mature green leaves of C. paliurus. The accumulation of glutamine inhibited phenylalanine biosynthesis by modulating the pentose phosphate pathway but promoted acetyl-CoA biosynthesis through the tricarboxylic acid cycle. These changes led to decreased flavonoid contents and increased triterpenoid contents in mature leaves. These metabolomic and transcriptomic data reveal the differential expression of metabolic regulatory networks during three stages of leaf development and highlight the trade-off between primary and secondary metabolism. Our results provide a comprehensive picture of the metabolic pathways that are active in the leaves of C. paliurus at different growth stages. [ABSTRACT FROM AUTHOR]

Nitrogen (N) is an essential macronutrient for plants, and its remobilization is key for adaptation to deficiency stress. However, there is limited understanding of the regulatory mechanisms of N remobilization in the important crop species Brassica napus (oilseed rape). Here, we report the identification of a transcription factor, BnaA9.WRKY47 , that is induced by N starvation in a canola variety. At the seedling stage, BnaA9.WRKY47 -overexpressing (OE) lines displayed earlier senescence of older leaves and preferential growth of juvenile leaves compared to the wild type under N starvation. At the field scale, the seed yield was significantly increased in the BnaA9.WRKY47 -OE lines compared with the wild type when grown under N deficiency conditions and, conversely, it was reduced in BnaA9.WRKY47 -knockout mutants. Biochemical analyses demonstrated that BnaA9.WRKY47 directly activates BnaC7.SGR1 to accelerate senescence of older leaves. In line with leaf senescence, the concentration of amino acids in the older leaves of the OE lines was elevated, and the proportion of plant N that they contained was reduced. This was associated with BnaA9.WRKY47 activating the amino acid permease BnaA9.AAP1 and the nitrate transporter BnaA2.NRT1.7. Thus, the expression of BnaA9.WRKY47 efficiently facilitated N remobilization from older to younger leaves or to seeds. Taken together, our results demonstrate that BnaA9.WRKY47 up-regulates the expression of BnaC7.SGR1 , BnaA2.NRT1.7 , and BnaA9AAP1 , thus promoting the remobilization of N in B. napus under starvation conditions. [ABSTRACT FROM AUTHOR]

The microRNA miR319 and its target JAW-TCP transcription factors regulate the proliferation-to-differentiation transition of leaf pavement cells in diverse plant species. In young Arabidopsis leaf primordia, JAW-TCPs are detected towards the distal region whereas the major mRNA319-encoding gene MIR319C, is expressed at the base. Little is known about how this complementary expression pattern of MIR319C and JAW-TCPs is generated. Here, we show that MIR319C is initially expressed uniformly throughout the incipient primordia and is later abruptly down-regulated at the distal region, with concomitant distal appearance of JAW-TCPs, when leaves grow to ~100 μm long. Loss of JAW-TCPs causes distal extension of the MIR319C expression domain, whereas ectopic TCP activity restricts MIR319C more proximally. JAW-TCPs are recruited to and are capable of depositing histone H3K27me3 repressive marks on the MIR319C chromatin. JAW-TCPs fail to repress MIR319C in transgenic seedlings where the TCP-binding cis-elements on MIR319C are mutated, causing miR319 gain-of-function-like phenotype in the embryonic leaves. Based on these results, we propose a model for growth patterning in leaf primordia wherein MIR319C and JAW-TCPs repress each other and divide the uniformly growing primordia into distal differentiation zone and proximal proliferation domain. Author summary: Young leaves in Arabidopsis exhibit a characteristic polar growth pattern where cells at the base grow due to continued cell division, while the tip stops growing as the cells exit division and enter differentiation. The MIR319C and its target JAW-TCP genes are expressed in the proximal and the distal domain respectively and are important for maintaining the polar growth pattern. However, it is not known how the complementary expression pattern of MIR319C and JAW-TCP genes is generated when leaves are initiated. Here, we show that MIR319C is expressed ubiquitously during leaf inception and later downregulated at the distal region. This distal loss of MIR319C is marked by an onset of JAW-TCP expression in the same domain. JAW-TCP proteins directly bind to, and downregulate the transcription of, the MIR319C gene to restrict its expression domain towards the base. Disruption of JAW-TCP-binding sites on the MIR319C promoter leads to miR319-overexpression-like phenotype. Together with the knowledge of miR319c-mediated post-transcriptional regulation of JAW-TCPs, we have shown that JAW-TCPs and miR319c mutually repress each other and generate growth polarity in early leaf primordia. [ABSTRACT FROM AUTHOR]

Solanaceous vegetable crops are cultivated and consumed worldwide. However, they often confront diverse abiotic stresses that significantly impair their growth, yield, and overall quality. This review delves into melatonin and abscisic acid (ABA) biosynthesis and their roles in abiotic stress responses. It closely examines the intricate interplay between melatonin and ABA in managing stress within plants, revealing both collaborative and antagonistic effects and elucidating the underlying molecular mechanisms. Melatonin and ABA mutually influence each other's synthesis, metabolism and that of other plant hormones, a key focus of this study. The study highlights melatonin's role in aiding stress management through ABA-dependent pathways and key genes in the melatonin-ABA interaction. Specifically, melatonin downregulates ABA synthesis genes and upregulates catabolism genes, leading to reduced ABA levels. It also directly scavenges H2O2, enhancing antioxidant enzyme activities, thereby underscoring their collaborative role in mediating stress responses. Moreover, the interplay between melatonin and ABA plays an essential role in multiple physiological processes of plants, including stomatal behaviors, wax accumulation, delay leaf senescence, seed germination, and seedlings growth, among others. Recognizing these relationships in Solanaceae vegetable crops holds great importance for improving agricultural practices and crop quality. In summary, this review offers a comprehensive overview of recent studies on the melatonin and ABA interplay, serving as a valuable resource for researchers and breeders dedicated to fortifying crop resilience and productivity within challenging environments. [ABSTRACT FROM AUTHOR]

Fatty Acid Desaturase 2 (FAD2) controls the conversion of oleic acids into linoleic acids. Mutations in FAD2 not only increase the high-oleic content, but also repress the leaf growth. However, the mechanism by which FAD2 regulates the growth pathway has not been elucidated in peanut leaves with single-cell resolution. In this study, we isolated fad2 mutant leaf protoplast cells to perform single-cell RNA sequencing. Approximately 24,988 individual cells with 10,249 expressed genes were classified into five major cell types. A comparative analysis of 3495 differentially expressed genes (DEGs) in distinct cell types demonstrated that fad2 inhibited the expression of the cytokinin synthesis gene LOG in vascular cells, thereby repressing leaf growth. Further, pseudo-time trajectory analysis indicated that fad2 repressed leaf cell differentiation, and cell-cycle evidence displayed that fad2 perturbed the normal cell cycle to induce the majority of cells to drop into the S phase. Additionally, important transcription factors were filtered from the DEG profiles that connected the network involved in high-oleic acid accumulation (WRKY6), activated the hormone pathway (WRKY23, ERF109), and potentially regulated leaf growth (ERF6, MYB102, WRKY30). Collectively, our study describes different gene atlases in high-oleic and normal peanut seedling leaves, providing novel biological insights to elucidate the molecular mechanism of the high-oleic peanut-associated agronomic trait at the single-cell level. [ABSTRACT FROM AUTHOR]

The early spring is a seasonal high-light "window" for new leaf growth and photosynthetic carbon capture by the shade-tolerant evergreen understory plants. However, it remains unclear how light regulates the source-sink relationship between rhizome (RO), mature leaf (ML), and immature leaf (IL) during Coptis chinensis leaf expansion. Understanding this relationship is essential to reducing RO reserve degradation and ultimately promote RO biomass accumulation. The plants grew in an artificial climate chamber with low (50 μmol m-2 s-1) and relatively high (200 μmol m-2 s-1) light intensity treatments. Leaf fluorescence, foliar phosphorus (P) fractions, soluble sugars, starch, total P, and alkaloid concentrations in ILs, MLs, and RO were measured, and 13C labeling was used to indicate the direction of photosynthetic carbon flow between organs. The plants grown under high light intensity had higher levels of starch in RO and higher RO biomass at the end of the year compared to those grown under low light intensity. The photosystemII (PSII) operating efficiency [Y(II)], relative electron transport rate (rETR), and photochemical quenching (qP), as well as sucrose and glucose, in ILs andMLs under relatively high light, was higher than those under low light. The glucose and starch concentrations in ILs at 35 d was significantly higher than that at 15 d when plants were under 200 μmol m-2 s-1, while they were not significantly changed and remained low at 50 μmol m-2 s-1. The 13C was detected in the RO when plants were grown at 200 μmol m-2 s-1, regardless of ILs and MLs 13C labeling, while no 13C was detected in the ROwhen plantswere under 50 μmol m-2 s-1. Additionally, the proportion of photosynthetic transport from ILs to MLs was significantly higher than that fromMLs to ILs under the 50-μmol m-2 s-1 limit. Total P concentration in ILs was lower under relatively high light, but there was no difference in nucleic acid P concentration in ILs under the two light intensity treatments. The alkaloid concentration in RO was lower under 200 μmol m-2 s-1 than that under 50 μmol m-2 s-1. We propose that relatively high light reduces the need for carbohydrates and P stored in the RO to support IL growth by (1) accelerating the sink-to-source transition in ILs, which inhibits the use of reserves in the RO; (2) using energy from MLs to support IL growth, thereby reducing RO reserve consumption, and (3) reducing the demand for P by investing less in the development of photosynthetic machinery. Furthermore, under low light, MLs serve as a sink and rely on other organs for support, directly or indirectly exacerbating the reserves lost in the RO. [ABSTRACT FROM AUTHOR]

Plant growth is a complex process affected by a multitude of genetic and environmental factors and their interactions. To identify genetic factors influencing plant performance under different environmental conditions, vegetative growth was assessed in Arabidopsis thaliana cultivated under constant or fluctuating light intensities, using high-throughput phenotyping and genome-wide association studies. Daily automated non-invasive phenotyping of a collection of 382 Arabidopsis accessions provided growth data during developmental progression under different light regimes at high temporal resolution. Quantitative trait loci (QTL) for projected leaf area, relative growth rate, and PSII operating efficiency detected under the two light regimes were predominantly condition-specific and displayed distinct temporal activity patterns, with active phases ranging from 2 d to 9 d. Eighteen protein-coding genes and one miRNA gene were identified as potential candidate genes at 10 QTL regions consistently found under both light regimes. Expression patterns of three candidate genes affecting projected leaf area were analysed in time-series experiments in accessions with contrasting vegetative leaf growth. These observations highlight the importance of considering both environmental and temporal patterns of QTL/allele actions and emphasize the need for detailed time-resolved analyses under diverse well-defined environmental conditions to effectively unravel the complex and stage-specific contributions of genes affecting plant growth processes. [ABSTRACT FROM AUTHOR]

The establishment of crop growth models enables the simulation of the impacts of environmental changes on crop growth, providing theoretical guidance for exploring the relationship between environmental factors and crop growth. The model used the growth cycle (GC) as the simulation time step and was built upon four submodels: topology, photosynthesis, biomass allocation, and geometric morphology. It was quantitatively utilized the concept of effective accumulated temperature (EAT) and parameters such as sink and expansion rate were used to explain the allometric growth relationships among different organs. The R2 values for the geometric morphological parameters such as leaf length, leaf width, leaf area, and internode volume ranged from 0.78 to 0.94, while the F-values for the regression equations ranged from 1533.53 to 13949.51. The R2 values for simulating leaf, internode, and earhead biomass were 0.62-0.94, 0.74-0.97, and 0.98, respectively, with RMSE values ranging from 0.02 to 0.13 g for leaf biomass, 0.03 to 0.13 g for internode biomass, and 1.71 g for earhead biomass. The results indicated that the model exhibited good performance and reliability in simulating the growth and development of leaves, internodes, and earheads. This provides a solid foundation for the development of a millet model with functional-structural feedback. [ABSTRACT FROM AUTHOR]

Onion (Allium cepa L) varieties grow at different latitudes. Onions need cool temperatures in the early stages of growth, and high temperatures and long days during bulbing. Sowing of seed in autumn, winter, and early spring results in more leaf growth and subsequently higher bulb yield than sowing in late-spring and summer. Onion varieties show different reactions to temperature and day length. Short-day varieties need shorter day lengths and low temperatures for bulb induction compared to long-day varieties. As the required minimum day length of an onion variety is met with increasing temperatures, bulbs will be initiated. Short-day varieties reached the bulbing phase at day lengths of 11–12 h. Cultivation of short-day varieties originating from low and equatorial latitudes in areas with higher latitudes resulted in early maturity. All onion varieties, regardless of photoperiod requirements, require low temperatures in the early growing season and seedling stage. Cultivars with similar photoperiod requirements in terms of morphological and genetic traits are located in different groups. Carbohydrates are transferred from leaves to bulbs over long days and during the bulbing stage. There is a close relationship between sugar and flowering genes like AcFT2. Region, variety, and genetics of varieties influence onion bulbing. [ABSTRACT FROM AUTHOR]

The Flowering locus T (FT) gene encodes the florigen protein, which primarily regulates the flowering time in plants. Recent studies have shown that FT genes also significantly affect plant growth and development. The FT gene overexpression in plants promotes flowering and suppresses leaf and stem development. This study aimed to conduct a transcriptome analysis to investigate the multiple effects of Jatropha curcas L. homolog (JcFT) overexpression on leaf growth in tobacco plants. The findings revealed that JcFT overexpression affected various biological processes during leaf development, including plant hormone levels and signal transduction, lipid oxidation metabolism, terpenoid metabolism, and the jasmonic-acid-mediated signaling pathway. These results suggested that the effects of FT overexpression in plants were complex and multifaceted, and the combination of these factors might contribute to a reduction in the leaf size. This study comprehensively analyzed the effects of JcFT on leaf development at the transcriptome level and provided new insights into the function of FT and its homologous genes. [ABSTRACT FROM AUTHOR]

In order to improve the leaf color and optimize the facility of cultivation system of Aglaonema commutatum ‘Big apple', its hydroponic seedlings were used as the test material in this study, six light qualities(white light, R:B = 1:1, R:B = 1:2, R:B = 2:1, R:B = 1:3, R:B = 3:1, in which R and B represent red light and blue light, respectively)and two photoperiods(8 h·d-1, 12 h·d-1)were cross cultured to determine the growth amount, biomass, soil and plant analyzer development(SPAD)value, anthocyanin content and leaf color parameters(hue value a*, hue value b*, luminosity L* and hue angle h*), and to investigate its response to red and blue light qualities and photoperiodic interactions. The results were as follows:(1)The dry weight and fresh weight of A. commutatum seedlings treated with LP11(R:B=1:3×12 h·d-1)were the highest, which was most conducive to plant growth and biomass accumulation; followed by LP5(R:B=1:3×8 h·d-1)treatment.(2)Under the same light quality condition, the SPAD value of 8 h·d-1 photoperiod was higher than that of 12 h·d-1 photoperiod, the anthocyanin content of 12 h·d-1 photoperiod was higher than that of 8 h·d-1 photoperiod, the SPAD value of LP11 treatment was the lowest and the anthocyanin content was the highest. The leaf color parameters a* and b* of A. commutatum cultured in 12 h·d-1 photoperiod were higher than those in 8 h·d-1 photoperiod, and the value of h* was lower than that in 8 h·d-1 photoperiod.(3)The principal component analysis showed that the LP11 treatment was the most effective in promoting the growth and leaf color improvement of A. commutatum. In conclusion, 12 h·d-1 photoperiod is more conducive to the growth and leaf color change of A. commutatum, and LP11 treatment is the best combination of light quality and photoperiod. [ABSTRACT FROM AUTHOR]

Quercus gilva is a precious timber tree species in middle subtropical China. In order to investigate the phenotypic diversity, phenotypic variation pattern and the major geographic and climatic factors influencing phenotypic variation of Q. gilva populations, 15 growth and leaf traits of 115 individuals from 14 natural populations were measured, and then the phenotypic diversity, variation pattern and the correlation between phenotypic variation and geographic and climatic factors were studied using nested analysis of variance, phenotypic differentiation coefficient, diversity index, correlation analysis, principal component analysis and cluster analysis. The results were as follows:(1)The average variation coefficient and the average Shannon-Wiener index of 15 phenotypic traits were 35.070% and 1.998, respectively. Variation coefficient and Shannon-Wiener index of 14 populations ranged from 14.94%(Dongkou)to 35.56%(Longshan)and from 1.127(Songyang)to 1.980(Changning), respectively. These results indicated that Q. gilva showed a relatively high level of phenotypic diversity.(2)Significant differences in 15 phenotypic traits were found among and within populations(P<0.01), with an average phenotypic differentiation coefficient of 41.491%. This result elucidated that the phenotypic variation within populations was greater than that among populations.(3)Correlation analysis showed that there were significant or entremely significant correlations among parts of the traits, whereas no correlation was found between phenotypic traits and geographic factors. Precipitation was the major factor that influenced the phenotypic traits of Q. gilva.(4)Principle component analysis indicated that the first four principal components explained 82.961% of total phenotypic contribution rate of Q. gilva.(5)Cluster analysis revealed that 14 Q. gilva populations were divided into three groups according to their different leaf and petiole size, such as small-leaved micropetiole, large-leaved macropetiole and medium-leaved micropetiole, which was not related to the geographic distribution of populations. This study provides scientific basis for the protection and utilization of Q. gilva germplasm resources, and lays an important foundation for its improved variety breeding. [ABSTRACT FROM AUTHOR]

In this paper, a biomimetic topology optimization design method that simulates the growth pattern of leaf veins is proposed for the design of the support structure of ultra-light airfoil-like solar cells in the solar powered unmanned aerial vehicle. This method simulates the optimal growth process of main vein morphology through the topology change of dynamic point groups to obtain an optimized topological main support structure and then generates a Voronoi grid structure in the area surrounded by the main support structure to increase the local support for the battery. The whole process is combined with genetic algorithm to simulate the optimal distribution strategy of leaf vein growth by inputting a small number of parameters. Compared with the traditional grid support structure, the support structure obtained by simulating the leaf vein growth optimization strategy can provide more efficient support for the solar panel and avoid damage to the solar cell. [ABSTRACT FROM AUTHOR]

The expansion of the vascular cambium cylinder in the stem of woody plants has been modeled many times, using different approaches and focusing on contributions of different cell events (cell divisions, intrusive cell growth and symplastic cell growth). Although there are many case studies in the literature, a universal model is still lacking. Therefore, the aim of this study is to estimate the quantitative changes in the contribution of symplastic growth of a single cambial cell (a sector of the cambial circumference) to the expansion of the vascular cambium cylinder, as the stem increases in girth. The proposed calculations, using the number π, and considering the actual dimensions of cambial cells, show (a) that the average symplastic increase per one initial cell in the circumferential direction decreases exponentially with the enlargement of cambial circumference, and (b) that the significant difference in the magnitude of symplastic increment of a single initial in the radial and circumferential directions increases proportionally to the increase in the circumference of the cambial cylinder. The proposed mathematical formula helps to understand the general rules that govern the gradual increase of the vascular cambium cylinder during wood production and would further facilitate the description/modeling of stem growth and formation of wood structural patterns. [ABSTRACT FROM AUTHOR]

Although the temporary presence of ribonucleotides in DNA is normal, their persistence represents a form of DNA damage. Here, we assess such damage and damage defense to DNA in plastids and mitochondria of maize. Shoot development proceeds from meristematic, non-pigmented cells containing proplastids and promitochondria at the leaf base to non-dividing green cells in the leaf blade containing mature organelles. The organellar DNAs (orgDNAs) become fragmented during this transition. Previously, orgDNA damage and damage defense of two types, oxidative and glycation, was described in maize, and now a third type, ribonucleotide damage, is reported. We hypothesized that ribonucleotide damage changes during leaf development and could contribute to the demise of orgDNAs. The levels of ribonucleotides and R-loops in orgDNAs and of RNase H proteins in organelles were measured throughout leaf development and in leaves grown in light and dark conditions. The data reveal that ribonucleotide damage to orgDNAs increased by about 2- to 5-fold during normal maize development from basal meristem to green leaf and when leaves were grown in normal light conditions compared to in the dark. During this developmental transition, the levels of the major agent of defense, RNase H, declined. The decline in organellar genome integrity during maize development may be attributed to oxidative, glycation, and ribonucleotide damages that are not repaired. [ABSTRACT FROM AUTHOR]

The leaf is the main site of photosynthesis and is an important component in shaping the ideal rice plant architecture. Research on leaf morphology and development will lay the foundation for high-yield rice breeding. In this study, we isolated and identified a novel curling leaf mutant, designated curling leaf 1 (cl1). The cl1 mutant exhibited an inward curling phenotype because of the defective development of sclerenchymatous cells on the abaxial side. Meanwhile, the cl1 mutant showed significant reductions in grain yield and thousand-grain weight due to abnormal leaf development. Through map-based cloning, we identified the CL1 gene, which encodes a MYB transcription factor that is highly expressed in leaves. Subcellular localization studies confirmed its typical nuclear localization. Transcriptome analysis revealed a significant differential expression of the genes involved in photosynthesis, leaf morphology, yield formation, and hormone metabolism in the cl1 mutant. Yeast two-hybrid assays demonstrated that CL1 interacts with alpha-tubulin protein SRS5 and AP2/ERF protein MFS. These findings provide theoretical foundations for further elucidating the mechanisms of CL1 in regulating leaf morphology and offer genetic resources for practical applications in high-yield rice breeding. [ABSTRACT FROM AUTHOR]

This study focuses on Sternbergia lutea (L.) Ker Gawl. ex Spreng., a bulbous, perennial, autumnal flowering geophyte mainly distributed around the Mediterranean Basin. The seasonal content of total sugars, starch and proline in above- and below-ground plant parts in this study, which has hitherto not been the subject of a published study. Geophytes possess underground storage organs that support sprouting, leaf growth and flowering. Furthermore, their buds remain protected below the soil surface during periods of dormancy. Understanding the fluctuation of these compounds in S. lutea contributes to our knowledge of its adaptation to the Mediterranean ecosystem. It seems likely that monthly fluctuations in proline accumulation, sugar and starch content in both above- and below-ground tissues of S. lutea correspond to the distinct seasonality of the Mediterranean ecosystem. Elevated starch content was investigated in the bulbs, while lower starch content was estimated in the leaves. Substantial values of soluble sugar content have been detected in bulbs and leaves. Additionally, elevated sugar content was detected in the yellow petals of S. lutea in October. Pronounced proline accumulation was detected in the leaves and bulbs of S. lutea during its active and dormant phase, respectively. [ABSTRACT FROM AUTHOR]

Copyright of Bulletin of Botanical Research is the property of Bulletin of Botanical Research Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Scindapsus (Araceae) is commonly found growing wild in Indonesian forests, including West Borneo. Scindapsus has been extensively cultivated and marketed, but information about the variability, morphology and growth of the plant is limited. This study aimed to identify five types of Indonesian Scindapsus based on their morphology, leaf anatomy, and growth patterns. The morphology study observed the plant's organ structures, including leaves, stems, and roots. The anatomy study examined the tissue structure of the leaf lamina, including epidermal cells and stomata. The growth observations were conducted by propagating the plants through stem cuttings. The research findings showed that, based on their morphology, the five types of Scindapsus in this study could be classified into two groups: Scindapsuspictus and Scindapsus treubii. The differences between the two groups were in the shape and pattern of their leaves. Based on leaf anatomy, structure, shape of the epidermal cells, and stomata types were the same for all five species, but Scindapsus treubii 2 has the thickest lamina. [ABSTRACT FROM AUTHOR]

Objectives: The objective of this data set was to identify transcriptional networks that control elongation of seedling leaf sheaths in the C4 grass Sorghum bicolor. One motivation was that leaf sheaths are a primary constituent of stems in grass seedlings; therefore, genes that control growth of this organ are important contributors to successful transition from the seedling stage to the mature plant stage and, ultimately, crop success. Since diurnal rhythms contribute to regulation of signaling networks responsible for growth, a time course representing the late afternoon and early evening was anticipated to pinpoint important control genes for stem growth. Ultimately, the expected outcome was discovery of transcript networks that integrate internal and external signals to fine tune leaf sheath growth and, consequently, plant height. Data description: The data set is RNAseq profiling of upper leaf sheaths collected from wild type Sorghum bicolor (BTx623 line) plants at four-hour intervals from 12.5 h after dawn to 20 h after dawn. Global transcript levels in leaves were determined by deep sequencing of mRNA from four individual seedlings at each time point. This data set contains sequences representing the spectrum of mRNAs from individual genes. This data set enables detection of significant changes in gene-level expression caused by the progression of the day from late afternoon to the middle of the night. This data set is useful to identify gene expression networks regulating growth in the leaf sheath, an organ that is a major contributor to the sorghum seedling stem and defines seedling height. [ABSTRACT FROM AUTHOR]

Background: Glandular trichomes, often referred to as "phytochemical factories", plays a crucial role in plant growth and metabolism. As the site for secretion and storage, the development of glandular trichomes is related to the dynamic biosynthesis of specialised metabolites. The study aims to explore the relationship between spatial phenotype and dynamic metabolism of glandular trichomes, and establish a novel approach for the exploration and study of the regulatory mechanism governing the development of glandular trichomes. Results: In this study, we proposed a technical route based on the relative deviation value to distinguish the peltate glandular trichomes (PGTs) from the background tissues and extract their spatial phenotype. By defining glandular trichome developmental stages based on the leaf vein growth axis, we found that young PGTs were densely distributed near the proximal end of growth axis of the leaf veins, where perillaketone, a primary metabolite of PGTs, is predominantly accumulated. Conversely, mature PGTs are typically found near the distal end of the mid-vein growth axis and the lateral end of the secondary vein growth axis, where the accumulation rate of isoegomaketone and egomaketone exceeds that of perillaketone in PGTs. We further identified spatial phenotypic parameters, Lsum and d, as independent variables to construct a linear regression model that illustrates the relationship between the spatial phenotypes and metabolite content of PGTs, including perillaketone (R2 = 0.698), egomaketone (R2 = 0.593), isoegomaketone (R2 = 0.662) and the sum of the amount (R2 = 0.773). Conclusions: This model proved that the development of PGTs was correlated with the growth of the entire leaf, and the development stage of PGTs can be identifined by spatial phenotypes based on the leaf veins. In conclusion, the findings of this study enhance our understanding of correlation between spatial phenotype and development of glandular trichomes and offer a new approach to explore and study the regulatory mechanism of glandular trichome development. [ABSTRACT FROM AUTHOR]

Studying intraspecific variation in multistress responses is central for predicting and managing the population dynamics of wild plant species under rapid global change. Yet, it remains a challenging goal in this field to integrate knowledge on the complex biochemical underpinnings for the targeted 'non-model' species. Here, we studied divergence in combined drought and heat responses among Northern and Southern European populations of the dune plant Cakile maritima , by combining comprehensive plant phenotyping with metabolic profiling via FT-ICR-MS and UPLC-TQ-MS/MS. We observed pronounced constitutive divergence in growth phenology, leaf functional traits, and defence chemistry (glucosinolates and alkaloids) among population origins. Most importantly, the magnitude of growth reduction under drought was partly weaker in southern plants and associated with divergence in plastic growth responses (leaf abscission) and the modulation of primary and specialized metabolites with known central functions not only in plant abiotic but also in biotic stress responses. Our study indicates that divergent selection has shaped the constitutive and drought-/heat-induced expression of numerous morphological and biochemical functional traits to mediate higher abiotic stress resistance in southern Cakile populations, and highlights that metabolomics can be a powerful tool to explore the underlying mechanisms of local adaptation in 'non-model' species. [ABSTRACT FROM AUTHOR]

Summary: A major advantage of using CRISPR/Cas9 for gene editing is multiplexing, that is, the simultaneous targeting of many genes. However, primary transformants typically contain hetero‐allelic mutations or are genetic mosaic, while genetically stable lines that are homozygous are desired for functional analysis. Currently, a dedicated and labor‐intensive effort is required to obtain such higher‐order mutants through several generations of genetic crosses and genotyping.We describe the design and validation of a rapid and efficient strategy to produce lines of genetically identical plants carrying various combinations of homozygous edits, suitable for replicated analysis of phenotypical differences. This approach was achieved by combining highly multiplex gene editing in Zea mays (maize) with in vivo haploid induction and efficient in vitro generation of doubled haploid plants using embryo rescue doubling.By combining three CRISPR/Cas9 constructs that target in total 36 genes potentially involved in leaf growth, we generated an array of homozygous lines with various combinations of edits within three generations. Several genotypes show a reproducible 10% increase in leaf size, including a septuple mutant combination.We anticipate that our strategy will facilitate the study of gene families via multiplex CRISPR mutagenesis and the identification of allele combinations to improve quantitative crop traits. [ABSTRACT FROM AUTHOR]

Plant organ growth results from the combined activity of cell division and cell expansion. The co-ordination of these two processes depends on the interplay between multiple hormones that determine the final organ size. Using the semidominant Hairy Sheath Frayed1 (Hsf1) maize mutant that hypersignals the perception of cytokinin (CK), we show that CK can reduce leaf size and growth rate by decreasing cell division. Linked to CK hypersignaling, the Hsf1 mutant has an increased jasmonic acid (JA) content, a hormone that can inhibit cell division. The treatment of wild-type seedlings with exogenous JA reduces maize leaf size and growth rate, while JA-deficient maize mutants have increased leaf size and growth rate. Expression analysis revealed the increased transcript accumulation of several JA pathway genes in the Hsf1 leaf growth zone. A transient treatment of growing wild-type maize shoots with exogenous CK also induced the expression of JA biosynthetic genes, although this effect was blocked by the co-treatment with cycloheximide. Together, our results suggest that CK can promote JA accumulation, possibly through the increased expression of specific JA pathway genes. [ABSTRACT FROM AUTHOR]

Arbuscular mycorrhizal (AM) fungi enhance plant stress tolerance, but it is unclear whether AM fungi affect heat tolerance in cucumbers. This study aimed to analyze how an AM fungus, Diversispora versiformis, affected growth, chlorophyll, five osmolytes, and plasma membrane intrinsic proteins (PIPs) and heat shock protein 70 (Hsp70) gene expression in cucumber leaves after a short-term (80 h) heat stress. Heat treatment significantly reduced root AM fungal colonization rate (0.26 folds). Heat treatment also distinctly suppressed plant height, stem diameter, and biomass, whereas AM fungal inoculation improved these growth variables as well as the chlorophyll index, with the benefit being more obvious under heat than under no-heat stress conditions. Heat treatment triggered differential changes in osmolytes (sucrose, fructose, and betaine) of inoculated and uninoculated cucumbers, whereas inoculation with AM fungus significantly raised leaf sucrose, fructose, glucose, betaine, and proline levels when compared to non-AM fungal inoculation. Heat treatment increased the expression of two (CsPIP1;6 and CsPIP2;1) of eight CsPIPs in inoculated and uninoculated plants, whereas AM fungal inoculation up-regulated the expression of CsPIP1;6, CsPIP2;1, and CsPIP2;6 under heat stress conditions. Hsp70s expressed differently in inoculated and uninoculated plants under heat versus no-heat stress, with 6 of 11 CsHsp70s down-regulated in inoculated plants. Under heat stress conditions, AM fungus only up-regulated CsHsp70-8 expression in 11 Hsp70s, while another eight CsHsp70s were down-regulated. Heat treatment and AM fungal inoculation both increased the expression of CsHsp70-8 and CsPIP1;6. It was concluded that AM fungus-inoculated cucumbers have high levels of growth, chlorophyll, and osmolytes under heat stress and do not require high CsPIPs and CsHsp70s expression to tolerate a short-term heat treatment. [ABSTRACT FROM AUTHOR]

Salt stress induces significant loss in crop yield worldwide. Although the growth‐stimulating effects of micronutrient nanoparticles (NPs) application under salinity have been studied, the molecular and biochemical mechanisms underlying these effects are poorly understood. The large size of maize leaf growth zones provides an ideal model system to sample and investigate the molecular and physiological bases of growth at subzonal resolution. Using kinematic analysis, our study indicated that salinity at 150 mM inhibited maize leaf growth by decreasing cell division and expansion in the meristem and elongation zones. Consistently, salinity downregulated cell cycle gene expression (wee1, mcm4, and cyclin‐B2‐4). B2O3 NP (BNP) mitigated the stress‐induced growth inhibition by reducing the decrease in cell division and expansion. BNP also enhanced the photosynthesis‐related parameters. Simultaneously, chlorophyll, phosphoenolpyruvate carboxylase and ribulose‐1,5‐bisphosphate carboxylase/oxygenase were stimulated in the mature zone. Concomitant with growth stimulation by BNP, mineral homeostasis, particularly for B and Ca, was monitored. BNP reduced oxidative stress (e.g., lessened H2O2 generation along the leaf zones and reduced lipid peroxidation in the mature zone) induced by salinity. This resulted from better maintenance of the redox status, that is, increased the glutathione‐ascorbate cycle in the meristem and elongation zones, and flavonoids and tocopherol levels in the mature zone. Our study has important implications for assessing the salinity stress impact mitigated by BNP on maize growth, providing a basis to improve the resilience of crop species under salinity stress conditions. [ABSTRACT FROM AUTHOR]

Plants contain three NADPH‐thioredoxin reductases (NTR) located in the cytosol/mitochondria (NTRA/B) and the plastid (NTRC) with important metabolic functions. However, mutants deficient in all NTRs remained to be investigated. Here, we generated and characterised the triple Arabidopsis ntrabc mutant alongside with ntrc single and ntrab double mutants under different environmental conditions. Both ntrc and ntrabc mutants showed reduced growth and substantial metabolic alterations, especially in sink leaves and under high CO2 (HC), as compared to the wild type. However, ntrabc showed higher effective quantum yield of PSII under both constant and fluctuating light conditions, altered redox states of NADH/NAD+ and glutathione (GSH/GSSG) and lower potential quantum yield of PSII in sink leaves in ambient but not high CO2 concentrations, as compared to ntrc, suggesting a functional interaction between chloroplastic and extra‐chloroplastic NTRs in photosynthesis regulation depending on leaf development and environmental conditions. Our results unveil a previously unknown role of the NTR system in regulating sink leaf metabolism and plant acclimation to HC, while it is not affecting full plant development, indicating that the lack of the NTR system can be compensated, at least to some extent, by other redox mechanisms. Summary Statement: We unveil that NTRC is crucial for sink leaf metabolism and plant acclimation to high CO2. Although Arabidopsis plants lacking all NADPH‐thioredoxin reductases (NTRs) have severely reduced growth, they produced viable seeds, indicating that NTRs are not essential for plant development and compensated by other redox mechanisms. [ABSTRACT FROM AUTHOR]

Maize (Zea mays), a cold‐sensitive crop, requires cold tolerance for extending the length of the growing season in temperate climates. However, response curves to different cold temperatures and exposure durations are lacking. We used a meta‐analysis approach using data from literature to investigate the effect of cold stress in the maize leaf. We constructed response curves to temperature and exposure durations for 18 key parameters related to leaf growth, photosynthesis, oxidative stress, antioxidants, and the phytohormone ABA. To determine their relevance for cold tolerance, we compared cold tolerant Flint and cold sensitive Dent lines. Treatment temperatures ranged from −20°C to 20°C for cold and from 12°C to 30°C for control and exposure duration from 3 min to 60 days. We found interacting effects of temperature and exposure durations on different response parameters. The strongest difference between Flint and Dent was observed for electrolyte leakage (EL). Our results show that the commonly used 4°C for cold and 25°C for control with medium cold exposure (1−7 days) induces a 50% decrease in shoot dry weight and leaf area and that EL is an easy and reliable indicator for cold tolerance studies. Summary Statement: Meta‐analysis of cold stress responses in the Zea mays leaf reveals differences in response curves of physiological parameters and shows that electrolyte leakage is a reliable indicator for cold tolerance. [ABSTRACT FROM AUTHOR]

Key message: Brassica orphan gene BrFLM, identified by two allelic mutants, was involved in leafy head formation in Chinese cabbage. Leafy head formation is a unique agronomic trait of Chinese cabbage that determines its yield and quality. In our previous study, an EMS mutagenesis Chinese cabbage mutant library was constructed using the heading Chinese cabbage double haploid (DH) line FT as the wild-type. Here, we screened two extremely similar leafy head deficiency mutants lfm-1 and lfm-2 with geotropic growth leaves from the library to investigate the gene(s) related to leafy head formation. Reciprocal crossing results showed that these two mutants were allelic. We utilized lfm-1 to identify the mutant gene(s). Genetic analysis showed that the mutated trait was controlled by a single nuclear gene Brlfm. Mutmap analysis showed that Brlfm was located on chromosome A05, and BraA05g012440.3C or BraA05g021450.3C were the candidate gene. Kompetitive allele-specific PCR analysis eliminated BraA05g012440.3C from the candidates. Sanger sequencing identified an SNP from G to A at the 271st nucleotide on BraA05g021450.3C. The sequencing of lfm-2 detected another non-synonymous SNP (G to A) located at the 266st nucleotide on BraA05g021450.3C, which verified its function on leafy head formation. We blasted BraA05g021450.3C on database and found that it belongs to a Brassica orphan gene encoding an unknown 13.74 kDa protein, named BrLFM. Subcellular localization showed that BrLFM was located in the nucleus. These findings reveal that BrLFM is involved in leafy head formation in Chinese cabbage. [ABSTRACT FROM AUTHOR]

【Objective】 The purpose was to study the effects of nitrogen application and leaves retention on the yield and quality of yellow sun-cured tobacco. 【Method】 Using the yellow sun-cured tobacco variety ‘Cunsanpi’ as the material, a split-area experimental design was adopted, and the two-factor experiments of nitrogen application of 82.5, 97.5, 112.5 and 127.5 kg/hm² and the number of leaves retained of 16, 19 and 22 leaves/plant (Total 12 treatments) were conducted to study its effects on the agronomic traits, yield, average price, yield value and appearance quality of yellow sun-cured tobacco. 【Result】 The results showed that increased nitrogen fertilization could promote the growth of tobacco plant and increase nitrogen metabolism, and at the same time, it was conducive to the improvement of agronomic traits such as stem girth, pitch, leaf length and width, and leaf area. Within a certain range of nitrogen application, tobacco yield increased with the increase in the number of leaves retained. Within a certain range of the number of leaves, the tobacco yield varied with the nitrogen application. When the number of leaves was 16 leaves/plant and 22 leaves/plant, the tobacco yield showed an upward trend with the increase of nitrogen application. When the number of retained leaves was 19 leaves/plant and the applied nitrogen ranged from 82.5 kg/hm² to 112.5 kg/hm², the tobacco yield increased with the increase of applied nitrogen. From the same level of nitrogen application of different number of leaves of tobacco leaf yield changes, when the nitrogen application of 127.5 kg/hm², the tobacco yield with the increase in the number of leaves of tobacco leaf is a downward trend. When the nitrogen application was 112.5 kg/hm² and the number of retained leaves was 19 leaves/plant, the tobacco leaf yield value was the highest, which was 54 567.00 yuan/hm². When the nitrogen applications were 82.5 kg/hm² and 97.5 kg/hm², the tobacco leaf yield value increased with the increase of the number of retained leaves. From the point of view of the average price of tobacco with different number of leaves of the same nitrogen application, when the nitrogen application was 82.5 kg/hm² and 127.5 kg/hm², the average price of tobacco showed a decreasing trend with the increase of the number of leaves of the same application. When the N application was 97.5 kg/hm², the average price of tobacco leaf increased with the increase of the number of retained leaves. When the nitrogen application was 112.5 kg/hm², the average price of tobacco leaf increased and then decreased with the number of leaves retained, and was higher in the treatment with 19 leaves/plant. The appearance quality of tobacco leaf was better in three treatments: 82.5 kg/hm² of nitrogen application, 16 leaves/plant; 97.5 kg/hm² of nitrogen application, 22 leaves/plant and 127.5 kg/hm² of nitrogen application, 19 leaves/plant. 【Conclusion】 Under the conventional cultivation condition of ensuring density of 15 150 plants/hm², applying nitrogen at 112.5 kg/hm² and retaining leaves at 19 leaves/plant, the yellow sun-cured tobacco variety ‘Cunsanpi’ could obtain better economic benefits in Fuquan, Guizhou. [ABSTRACT FROM AUTHOR]

C3 and C4 grasses directly and indirectly provide the vast majority of calories to the human diet, yet our understanding of the molecular mechanisms driving photosynthetic productivity in grasses is largely unexplored. Ground meristem cells divide to form mesophyll or vascular initial cells early in leaf development in C3 and C4 grasses. Here we define a genetic circuit composed of SHORT ROOT (SHR), INDETERMINATE DOMAIN (IDD), and PIN-FORMED (PIN) family members that specifies vascular identify and ground cell proliferation in leaves of both C3 and C4 grasses. Ectopic expression and loss-of-function mutant studies of SHR paralogs in the C3 plant Oryza sativa (rice) and the C4 plant Setaria viridis (green millet) revealed the roles of these genes in both minor vein formation and ground cell differentiation. Genetic and in vitro studies further suggested that SHR regulates this process through its interactions with IDD12 and 13. We also revealed direct interactions of these IDD proteins with a putative regulatory element within the auxin transporter gene PIN5c. Collectively, these findings indicate that a SHR–IDD regulatory circuit mediates auxin transport by negatively regulating PIN expression to modulate minor vein patterning in the grasses. A SHORT ROOT–INTERMEDIATE DOMAIN regulatory circuit modulates auxin transport by negatively regulating PIN-FORMED expression to regulate minor vein patterning in grasses. [ABSTRACT FROM AUTHOR]

The nutrient filter function is an important ecosystem service of seagrass meadows that mitigates the consequences of coastal eutrophication. In northeast Hainan in China, large seagrass areas were lost due to chronic eutrophication induced by untreated pond aquaculture effluents. However, in adjacent areas, seagrasses could survive due to seasonal exposure (i.e., not chronic) to eutrophication only. In a way, the conditions in these areas represent a transitional environment which allows investigating the effect of eutrophication on seagrass performance and their nitrogen uptake capacity. We tested how a 4‐week in situ nutrient enrichment affected inorganic nitrogen uptake rates of a multispecies seagrass meadow in eutrophic and non‐eutrophic seasons, in light and in darkness. All species maintained nitrogen uptake in the dark and preferred ammonium over nitrate. In the eutrophic season, the seagrass leaf biomass and growth were lower resulting in a lower nitrogen filter capacity. Among the species present, Cymodocea rotundata and Cymodocea serrulata covered 48% and 45%, respectively, of their daily nitrogen demand for leaf growth through leaf uptake from the water column, while it was only 30% for Thalassia hemprichii, the last remaining species in meadows degraded by eutrophication, as deduced from previous studies. It indicates that a multispecies seagrass meadow has a higher nitrogen filter capacity than a monospecific T. hemprichii meadow. By reducing seagrass diversity and, hence, the nitrogen filter function, eutrophication triggers a self‐reinforcing process. Once the nitrogen filtering capacity of a seagrass meadow is exhausted, further eutrophication and seagrass loss are expected. [ABSTRACT FROM AUTHOR]

Phytohormones are key signalling molecules in developing responses in plants with various biotic and abiotic stresses. From the catalogue of well-known classical plant hormones, Jasmonic acid (JA) and its corresponding precursors and derivatives attract massive research attention by acting as potential methods for improving tolerance in diseased plants. Jasmonates are oxylipins chiefly derived from α-linolenic acids through the octadecanoid pathway. Plants synthesise this hormone in response to growth or defence-linked signs to coordinate plant development, growth or defence against numerous pathogenic microorganisms. Several reports emphasise the contribution of JAs in regulating vital physiological processes such as leaf senescence, tuber formation, photosynthesis, reproduction, seed germination and growth inhibition in plants. In response to pathogen exposure, JAs assume their operational task as a 'master switch', in charge of the initiation of signal transduction cascade, participating in the upregulation of genes associated with the production of alkaloids and phytoalexin, synthesis and accumulation of storage proteins, cell wall components and most importunately—stress ameliorative agents. This review focuses on recent findings connected to the structure, biosynthesis, regulation and signalling systems of JAs. In addition to this, the present article gives an essential insight into how the application of this phytohormone is involved in stress amelioration and induction of pathogenesis-resistant genes from an agriculturist, plant physiologist and biotechnologist's point of view. [ABSTRACT FROM AUTHOR]

Plant senescence is mediated by complex regulatory pathways. Any delay in the onset of senescence is linked with improved plant growth and fitness. In this work, we identified Solanum lycopersicum ACAULIS5 (SlACL5) gene, a close homolog of Arabidopsis thaliana ACAULIS5 (AtACL5), which shows higher expression in developing leaves, stem, and roots of tomato plants. We also observed that its expression was gradually decreased from young and mature leaves to leaves undergoing senescence. We demonstrated that its constitutive overexpression (35S::SlACL5) in Nicotiana tabacum resulted in increased plant growth and higher accumulation of pigments and delayed the senescence of leaves. We found that the average length of plants of 35S::SlACL5 OE lines was higher than the control plants. The anatomy of the stem of OE lines showed no major alterations in cortical and vascular tissues. However, the expression of xylem cell death marker NtMC9 was downregulated in these lines indicating delayed xylem cell death program. Furthermore, we observed that expression of early-stage and late-stage senescence-associated marker genes, NtSGR1 and NtSAG12 was greatly reduced in the leaves of OE lines showing delayed senescence process in the leaves. We show that OE plants have reduced levels of Reactive Oxygen Species (ROS) and activity of some of the antioxidant enzymes was reduced or misregulated. The MDA content was also found to be less in these lines. Relative chloroplast DNA (cpDNA) content and marker gene expression analysis also performed which show higher cpDNA content and increased expression of chloroplast marker genes, NtrpoA, NtrbcL, and NtpetB in OE lines suggesting higher chloroplast activity in OE lines than the control. We conclude that overexpression of SlACL5 in tobacco leads to altered plant development, higher chloroplast activity and chlorophyll contents, reduced ROS generation, and downregulated expression of senescence-associated marker genes, thereby delaying leaf senescence program which resulted in vigorous plant growth. [ABSTRACT FROM AUTHOR]

Introduction: Leaves are important organs for photosynthesis in plants, and the restriction of leaf growth is among the earliest visible effects under abiotic stress such as nutrient deficiency. Rapidly and accurately monitoring plant leaf area is of great importance in understanding plant growth status in modern agricultural production. Method: In this paper, an image processing-based non-destructive monitoring device that includes an image acquisition device and image process deep learning net for acquiring Brassica napus (rapeseed) leaf area is proposed. A total of 1,080 rapeseed leaf image areas from five nutrient amendment treatments were continuously collected using the automatic leaf acquisition device and the commonly used area measurement methods (manual and stretching methods). Results: The average error rate of the manual method is 12.12%, the average error rate of the stretching method is 5.63%, and the average error rate of the splint method is 0.65%. The accuracy of the automatic leaf acquisition device was improved by 11.47% and 4.98% compared with the manual and stretching methods, respectively, and had the advantages of speed and automation. Experiments on the effects of the manual method, stretching method, and splinting method on the growth of rapeseed are conducted, and the growth rate of rapeseed leaves under the stretching method treatment is considerably greater than that of the normal treatment rapeseed. Discussion: The growth rate of leaves under the splinting method treatment was less than that of the normal rapeseed treatment. The mean intersection over union (mIoU) of the UNet-Attention model reached 90%, and the splint method had higher prediction accuracy with little influence on rapeseed. [ABSTRACT FROM AUTHOR]

The modern agriculture system based on open-field crops requires a lot of energy and resources in terms of soil, water, and chemicals. Vertical farming (VF) systems could be a viable alternative for some types of cultivation that are receiving interest thanks to their high modularity, optimized water and nutrients use, and LEDs employment as an energy-efficient light source. However, VF design and installation are expensive and require well-tailored optimization depending on the specific crop to increase its competitiveness. This work analyzed the effects of different combinations of NPK (nitrogen-phosphorus-potassium) slow-release fertilizers and LED-based light recipes on the growth of baby leaf lettuce (Lactuca sativa L.), taking advantage of the Design of Experiments (DoE) methodology. The type of slow-release fertilizer, its quantity measured as the number of aggregates from 0 to 6, and the type of light recipe were considered as input factors, and their possible influence on the growth of lettuce (in terms of morphological parameters) in a controlled indoor farming system was measured. Results suggest that using higher fertilizer inputs equal to six aggregates leads to an increase of average leaf area equal to 46% (from 13.00 cm2 to 19.00 cm2), while the fresh weight of lettuce increases by 65% (from 1.79 g to 2.96 g). However, the height of plants also depends on the combination of the light recipes. In particular, the separate coupling of higher inputs of two fertilizers and light recipes leads to an increase in the height of lettuce equal to 33% (from 6.00 cm to 8.00 cm). [ABSTRACT FROM AUTHOR]

Plants contain three NADPH‐thioredoxin reductases (NTR) located in the cytosol/mitochondria (NTRA/B) and the plastid (NTRC) with important metabolic functions. However, mutants deficient in all NTRs remained to be investigated. Here, we generated and characterised the triple Arabidopsis ntrabc mutant alongside with ntrc single and ntrab double mutants under different environmental conditions. Both ntrc and ntrabc mutants showed reduced growth and substantial metabolic alterations, especially in sink leaves and under high CO2 (HC), as compared to the wild type. However, ntrabc showed higher effective quantum yield of PSII under both constant and fluctuating light conditions, altered redox states of NADH/NAD+ and glutathione (GSH/GSSG) and lower potential quantum yield of PSII in sink leaves in ambient but not high CO2 concentrations, as compared to ntrc, suggesting a functional interaction between chloroplastic and extra‐chloroplastic NTRs in photosynthesis regulation depending on leaf development and environmental conditions. Our results unveil a previously unknown role of the NTR system in regulating sink leaf metabolism and plant acclimation to HC, while it is not affecting full plant development, indicating that the lack of the NTR system can be compensated, at least to some extent, by other redox mechanisms. Summary Statement: We unveil that NTRC is crucial for sink leaf metabolism and plant acclimation to high CO2. Although Arabidopsis plants lacking all NADPH‐thioredoxin reductases (NTRs) have severely reduced growth, they produced viable seeds, indicating that NTRs are not essential for plant development and compensated by other redox mechanisms. [ABSTRACT FROM AUTHOR]

Overfeeding and a lack of exercise are increasingly causing obesity in dogs, which has become a big problem threatening the health of dogs. Therefore, it is necessary to investigate how dietary regulations can help to improve dogs' body conditions and minimize obesity. This study was carried out to investigate the effects of dietary mulberry leaf powder (MLP) supplementation on the growth performance, lipid metabolism parameters, and gut microbiota of Chinese indigenous dogs. Fifteen Chinese indigenous dogs (6.34 ± 0.56 kg) were randomly assigned to three treatment groups and received either the control diet (CON), high-fat diet (HF), or high-fat diet containing 6% Mulberry leaf powder (MLP) for four weeks. The CON group received a basal diet, the HF group received a basal diet supplemented with 10% lard, and the MLP group received a basal diet supplemented with 10% lard and 6% MLP. The trial lasted for four weeks. The growth performance, lipid metabolism parameters, immune globulins, cytokines, and fecal microbiota were measured. Results showed that there was no significant difference in growth performance. The MLP group appeared to have decreased (p < 0.05) the serum level of low-density lipoprotein cholesterol (LDL-C) and apoliprotein-A1(APO-A1) in serum. The MLP group appeared to have higher (p < 0.05) serum immune globulin A (IgA) levels. UPGMA results showed that the MLP group was closer to the CON group than to the HF group. LEfSe analysis showed that dietary supplementation with MLP contributed to an alteration in the genus Alloprevotella, Sarcina, and species belonging to the Bacteroides and Lactobacillus genus. Overall, the dietary supplementation of 6% MLP can improve lipid metabolism conditions and immunity in high-fat-diet-fed dogs, and can alter the gut microbial composition of dogs. [ABSTRACT FROM AUTHOR]

Sorghum is an essential crop for resilient and adaptive responses to climate change. The root systems of crop plants significantly contribute to the tolerance of abiotic stresses. There is little information on sorghum genotypes' root systems and plasticity to external P supply. In this paper, we investigated the variations in root systems, as well as the responses, trait relationships, and plasticity of two sorghum genotypes (Naga Red and Naga White), popularly grown in Ghana, to five external P concentrations ([P]ext): 0, 100, 200, 300, and 400 mg P kg−1 soil. Sorghum plants were grown in greenhouse pots and harvested for root trait measurements at the five‐leaf and growing point differentiation (GPD) developmental stages. The plants were responsive to [P]ext and formed rhizosheaths. The two genotypes showed similar characteristics for most of the traits measured but differed significantly in total and lateral root lengths in favor of the red genotype. For example, at the five‐leaf growth stage, the lateral root length of the red and white genotypes was 22.8 and 16.2 cm, respectively, but 124 and 88.9 cm, at the GPD stage. The responses and plasticity of the root system traits, including rhizosheath, to [P]ext were more prominent, positive, and linear at the five‐leaf stage than at the GPD growth stage. At the five‐leaf growth stage, total root length increased by about 2.5‐fold with increasing [P]ext compared to the unamended soil. At the GPD stage, however, total root length decreased by about 1.83‐fold as [P]ext increased compared to the unamended soil. Specific rhizosheath weight correlated with RHD, albeit weakly, and together explained up to 59% of the variation in tissue P. Root hair density was more responsive to P supply than root hair length and showed a similar total and lateral root length pattern. Most desirable responses to P occurred at a rate of 200–300 mg P kg−1 soil. It is concluded that sorghum would form rhizosheath, and [P]ext could be critical for the early vigorous growth of sorghum's responsive root and shoot traits. Beyond the early days of development, additional P application might be necessary to sustain the responses and plasticity observed during the early growth period, but this requires further investigation, potentially under field conditions. [ABSTRACT FROM AUTHOR]

Inherently, ammonium (NH4+) is critical for plant growth; however, its toxicity suppresses potassium (K+) uptake and vice-versa. Hence, attaining a nutritional balance between these two ions (NH4+ and K+) becomes imperative for the growth of tobacco seedlings. Therefore, we conducted a 15-day experimental study on tobacco seedlings exposed to different concentrations (47 treatments) of NH4+/K+ at different corresponding 12 ratios simultaneously in a hydroponic system. Our study aimed at establishing the optimal NH4+-K+ concentration and the corresponding ratio required for optimal growth of different tobacco plant organs during the seedling stage. The controls were the baseline for comparison in this study. Plants with low or excessive NH4+-K+ concentration had leaf chlorosis or dark greenish colouration, stunted whole plant part biomass, and thin roots. We found that adequate K+ supply is a pragmatic way to mitigate NH4+-induced toxicity in tobacco plants. The optimal growth for tobacco leaf and root was attained at NH4 +-K+ concentrations 2-2 mM (ratio 1:1), whereas stem growth was optimal at NH4+-K+ 1-2 mM (1:2). The study provided an insight into the right combination of NH4+/K+ that could mitigate or prevent NH4+ or K+ stress in the tobacco seedlings. [ABSTRACT FROM AUTHOR]

BASIC PENTACYSTEINE (BPCs) transcription factors are important regulators of plant growth and development. However, the regulatory mechanism of BPC2 in roots remains unclear. In our previous study, we created Csbpc2 cucumber mutants by the CRISPR/Cas9 system, and our studies on the phenotype of Csbpc2 mutants showed that the root growth was inhibited compared with wide‐type (WT). Moreover, the surface area, volume and number of roots decreased significantly, with root system architecture changing from dichotomous branching to herringbone branching. Compared with WT, the leaf growth of the Csbpc2 mutants was not affected. However, the palisade and spongy tissue were significantly thinner, which was not beneficial for photosynthesis. The metabolome of root exudates showed that compared with WT, amino acids and their derivatives were significantly decreased, and the enriched pathways were mainly regulated by amino acids and their derivatives, indicating that knockout of CsBPC2 mainly affected the amino acid content in root exudates. Importantly, transcriptome analysis showed that knockout of CsBPC2 mainly affected root gene expression. Knockout of CsBPC2 significantly reduced the gene expression of gibberellins synthesis. However, the expression of genes related to amino acid synthesis, nitrogen fixation and PSII‐related photosynthesis increased significantly, which may be due to the effect of knocking out CsBPC2 on gibberellins synthesis, resulting in the inhibition of seedling growth, thus forming negative feedback regulation. Generally, we showed for the first time that BPC2 is a key regulator gene of root growth and development, laying the foundation for future mechanisms of BPC2 regulation in roots. [ABSTRACT FROM AUTHOR]