Search

Your search keyword '"Kim, Junghyun"' showing total 1,700 results
Start Over Author "Kim, Junghyun"
1,700 results on '"Kim, Junghyun"'

217. Mechano-NPS and Visco-NPS: Microfluidic Approaches to Single-Cell Mechanics

Author

Kim, Junghyun

Subjects
Biomedical engineering, Biomechanics
Abstract

The mechanical properties of cells provide valuable information regarding biological and clinically relevant cellular characteristics. In this dissertation, we demonstrate two new microfluidic platforms, mechano-node-pore sensing (mechano-NPS) and visco-node-pore sensing (visco-NPS), to characterize cellular mechanical behavior. Mechano-NPS is a multi- parametric single-cell-analysis method to quantify simultaneously cell diameter, resistance to compressive deformation, transverse deformation under constant strain, and recovery time after deformation. We define a new parameter, the whole-cell deformability index (wCDI), which provides a quantitative mechanical metric of the resistance to compressive deformation that can be used to discriminate among different cell types. The wCDI and the transverse deformation under constant strain show malignant MCF-7 and A549 cell lines are mechanically distinct from non-malignant, MCF-10A and BEAS-2B cell lines. We categorize cell recovery time and show that the composition of recovery types, which is a consequence of changes in cytoskeletal organization, correlates with cellular transformation. Through the wCDI and cell-recovery time, mechano-NPS discriminates between sub-lineages of normal primary human mammary epithelial cells. Mechano-NPS identifies mechanical phenotypes that distinguishes lineage, chronological age, and stage of malignant progression in human epithelial cells.Visco-NPS is a new, electronic-based, microfluidic rheology platform that quantifies cellular viscoelastic properties under periodic deformation. We measure the storage (G’) and loss (G”) modulus of individual cells, which represent cellular elasticity and viscosity, respectively. By applying a wide range of deformation frequency, our platform quantifies the frequency dependency of viscoelastic properties. The measurement of G’ and G” shows that malignant breast epithelial (MCF-7) cells have distinctly different viscoelastic properties as compared to non-malignant breast epithelial (MCF-10A) cells. With its sensitivity, visco-NPS is able to dissect the individual contributions of different cytoskeletal components, i.e. actin filaments and microtubules, to whole-cell mechanical properties. Through G’ and G”, visco-NPS can also quantify the mechanical transitions—a consequence of changes in cytoskeletal organization and nucleus structure—that cells undergo as they traverse the cell cycle. visco-NPS identifies viscoelastic characteristics of cells, which can provide both a biophysical understanding of cellular behavior and a potential for clinical applications.

Published
2018

219. Development of LMS/LCMS (Contents Link Module) Real-Time Interactive in Videos for Maximizing the Effect of Learning

Author

Kim, Junghyun, Hwang, Doohong, Kim, Kangseok, Jung, Changduk, Kim, Wonil, Hutchison, David, Series editor, Kanade, Takeo, Series editor, Kittler, Josef, Series editor, Kleinberg, Jon M., Series editor, Mattern, Friedemann, Series editor, Mitchell, John C., Series editor, Naor, Moni, Series editor, Nierstrasz, Oscar, Series editor, Pandu Rangan, C., Series editor, Steffen, Bernhard, Series editor, Sudan, Madhu, Series editor, Terzopoulos, Demetri, Series editor, Tygar, Doug, Series editor, Vardi, Moshe Y., Series editor, Weikum, Gerhard, Series editor, Gong, Zhiguo, editor, Luo, Xiangfeng, editor, Chen, Junjie, editor, Lei, Jingsheng, editor, and Wang, Fu Lee, editor

Published
2011
Full Text
View/download PDF

220. A Cartoon Image Classification System Using MPEG-7 Descriptors

Author

Kim, Junghyun, Baik, Sung Wook, Kim, Kangseok, Jung, Changduk, Kim, Wonil, Hutchison, David, Series editor, Kanade, Takeo, Series editor, Kittler, Josef, Series editor, Kleinberg, Jon M., Series editor, Mattern, Friedemann, Series editor, Mitchell, John C., Series editor, Naor, Moni, Series editor, Nierstrasz, Oscar, Series editor, Pandu Rangan, C., Series editor, Steffen, Bernhard, Series editor, Sudan, Madhu, Series editor, Terzopoulos, Demetri, Series editor, Tygar, Doug, Series editor, Vardi, Moshe Y., Series editor, Weikum, Gerhard, Series editor, Goebel, Randy, editor, Siekmann, Jörg, editor, Wahlster, Wolfgang, editor, Deng, Hepu, editor, Miao, Duoqian, editor, Lei, Jingsheng, editor, and Wang, Fu Lee, editor

Published
2011
Full Text
View/download PDF

224. Epstein-Barr virus gH/gL has multiple sites of vulnerability for virus neutralization and fusion inhibition

Author

Chen, Wei-Hung, primary, Kim, JungHyun, additional, Bu, Wei, additional, Board, Nathan L., additional, Tsybovsky, Yaroslav, additional, Wang, Yanmei, additional, Hostal, Anna, additional, Andrews, Sarah F., additional, Gillespie, Rebecca A., additional, Choe, Misook, additional, Stephens, Tyler, additional, Yang, Eun Sung, additional, Pegu, Amarendra, additional, Peterson, Caroline E., additional, Fisher, Brian E., additional, Mascola, John R., additional, Pittaluga, Stefania, additional, McDermott, Adrian B., additional, Kanekiyo, Masaru, additional, Joyce, M. Gordon, additional, and Cohen, Jeffrey I., additional

Published
2022
Full Text
View/download PDF

227. Prognostic Factors for Pulmonary Fibrosis Following Pneumonia in Patients with COVID-19: A Prospective Study

Author

Lee, Inhan, primary, Kim, Joohae, additional, Yeo, Yohwan, additional, Lee, Ji Yeon, additional, Jeong, Ina, additional, Joh, Joon-Sung, additional, Kim, Gayeon, additional, Chin, Bum Sik, additional, Kim, Yeonjae, additional, Kim, Min-Kyung, additional, Jeon, Jaehyun, additional, Yoon, Yup, additional, Jin, Sung Chan, additional, and Kim, Junghyun, additional

Published
2022
Full Text
View/download PDF

236. Domain Level Page Sharing in Xen Virtual Machine Systems

Author

Jeon, Myeongjae, Seo, Euiseong, Kim, Junghyun, Lee, Joonwon, Hutchison, David, editor, Kanade, Takeo, editor, Kittler, Josef, editor, Kleinberg, Jon M., editor, Mattern, Friedemann, editor, Mitchell, John C., editor, Naor, Moni, editor, Nierstrasz, Oscar, editor, Pandu Rangan, C., editor, Steffen, Bernhard, editor, Sudan, Madhu, editor, Terzopoulos, Demetri, editor, Tygar, Doug, editor, Vardi, Moshe Y., editor, Weikum, Gerhard, editor, Xu, Ming, editor, Zhan, Yinwei, editor, Cao, Jiannong, editor, and Liu, Yijun, editor

Published
2007
Full Text
View/download PDF

237. A 39/48 GHz Switchless Reconfigurable Low Noise Amplifier Using Common Gate and Coupled-Line-Based Diplexer

Author

Ko, Byunghun, Lee, Sunwoo, Seo, Wonwoo, Sim, Taejoo, Kim, Sunghyuk, Kim, Min-Su, and Kim, Junghyun

Abstract

This brief presents a switchless dual-band low-noise amplifier (LNA) that can be reconfigured to include n260 (37–40 GHz) and n262 (47.2–48.2 GHz) millimeter-wave (mm-Wave) dual bands. The proposed reconfigurable LNA is designed to separate and isolate two bands, using a common gate and coupled-line-based diplexer for the inter-stage of cascade amplifiers. A common gate amplifier has the advantage of simultaneously selecting the signal path and amplifying the signal power according to a band, using an impedance variation between the “on/off” conditions of gate bias. In addition, the coupled-line-based diplexer can be optimized to exhibit high isolation characteristics between frequencies using high and low-pass characteristics. The designed reconfigurable LNA is fabricated with the 0.15- $\boldsymbol{\mu }\mathbf {m}$ GaAs pHEMT process. With the high frequency (HF) mode, the reconfigurable LNA achieves a measured gain of 19.2 dB at the center frequency, noise figure, and ${\mathrm{ OP}}_{\mathrm{ 1dB}}$ of 48 GHz, 4.8 dB, and 6.88 dBm, respectively. In the low frequency (LF) mode, the reconfigurable LNA exhibits a measured gain of 24.8 dB at the center frequency, noise figure, and ${\mathrm{ OP}}_{\mathrm{ 1dB}}$ of 39 GHz, 3.9 dB, and 6.80 dBm, respectively. The chip occupies a core area of $3.04~{mm}^{2}$ , which consumes $61.4~\mathbf {mW}$ and $64.7~\mathbf {mW}$ in the HF and LF modes, respectively.

Published
2023
Full Text
View/download PDF

239. Exploiting Question Concepts for Query Expansion

Author

Kim, Hae-Jung, Bu, Ki-Dong, Kim, Junghyun, Lee, Sang-Jo, Hutchison, David, editor, Kanade, Takeo, editor, Kittler, Josef, editor, Kleinberg, Jon M., editor, Mattern, Friedemann, editor, Mitchell, John C., editor, Naor, Moni, editor, Nierstrasz, Oscar, editor, Pandu Rangan, C., editor, Steffen, Bernhard, editor, Sudan, Madhu, editor, Terzopoulos, Demetri, editor, Tygar, Dough, editor, Vardi, Moshe Y., editor, Weikum, Gerhard, editor, and Gelbukh, Alexander, editor

Published
2005
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results