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This experimental analogue study estimated how clients' ambivalence over emotional expression (AEE) is linked to their perception of the therapeutic relationship and satisfaction with counseling, depending on the counselor's communication style (i.e., directive versus non-directive). One hundred and seventy three college students were recruited using a research participation pool. Participants viewed videos of mock counseling sessions that demonstrated a counselor with a directive or non-directive communication style and were instructed to imagine they were the client in the video. Participants then rated their perceived therapeutic bond and satisfaction with the counseling in the video. Results from a moderated mediation effect analysis indicated that the counselor's directiveness moderated the relationship between AEE and perceived therapeutic bond, which in turn mediated the relationship between AEE and counseling satisfaction. Specifically, participants with higher levels of AEE reported lower levels of satisfaction when they watched a non-directive style of therapy session, and this relationship was mediated by lower levels of perceived therapeutic bond. However, this association was not found for the directive style video. Implications and future directions for research and practice are discussed. [ABSTRACT FROM AUTHOR]

Keywords: Fluorescent probe; Indolizine; Fluoride sensor; Bioimaging EN Fluorescent probe Indolizine Fluoride sensor Bioimaging 95 98 4 01/29/21 20210101 NES 210101 Schematic representation of fluoride anion sensing with Silyl-KIz in live cell GLO:1CJF/01jan21:bkcs12134-toc-0001.jpg PHOTO (COLOR): . gl Fluoride ions are used widely in toothpaste, pharmaceutical agents, and even drinking water for the prevention of dental caries and the amelioration of osteoporosis.1 However, a concentration of fluoride above 1.5 mg/mL (79 M) in drinking water can cause dental and/or skeletal fluorosis, as defined by the WHO.2 Therefore, there is a strong demand for new methods for detecting fluoride ions.3 Although numerous analytical methods have been developed for fluoride sensing, including mass spectrometry, atomic absorption spectrophotometry, ion-selective electrode analysis, nuclear magnetic resonance (NMR) spectroscopy, and ion chromatography,4 the requirement for inconvenient equipment and/or sophisticated procedures limits the accessibility of the analysis.5 To overcome these limitations, researchers have continued to explore the development of fluorescent probes for fluoride ions.6-8 However, it is still a challenge to develop a practical fluorescent probe for these ions in live cells. To confirm that Silyl-KIz could be used as a fluorescent fluoride sensor, the emission intensity of the Silyl-KIz solution in 1× PBS in the presence of different fluoride ion concentrations was measured (Figure 3). To demonstrate the suitability of Silyl-KIz for biological applications, we investigated whether Silyl-KIz could sensitively detect fluoride ions in live HeLa cells. [Extracted from the article]