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Balloon-borne observations of lower stratospheric water vapor were conducted with the Cryogenic Frostpoint Hygrometer (CFH) in July, September, and November 2013 at Syowa Station (69.0oS, 39.6oE) in the Antarctic. High-precision and high vertical resolution data of water vapor concentration up to an altitude of about 28 km were obtained successfully except for a contamination in the observation of July 2013. A comparison between the CFH and coincident satellite (i.e., Aura/MLS) observations showed a good agreement within their uncertainty. A position of Syowa Station relative to the stratospheric polar vortex edge varied depending on both the observation date and altitude. Temperature and pressure histories of the observed air parcels were examined by 10-day backward trajectories. These analyses clearly demonstrated that most air parcels observed in the lower stratosphere above Syowa Station experienced final dehydration inside the polar vortex. On the other hand, a clear signature of rehydration or incomplete dehydration was also observed around a 25 hPa pressure level in the observation of July 2013.

Inertia-gravity waves (IGWs) are an important component for the dynamics of the middle atmosphere. However, observational studies needed to constrain their forcing are still insufficient especially in the remote areas of the Antarctic region. One year of observational data (January to December 2013) by the PANSY radar of the wind components (vertical resolution of 150 m and temporal resolution of 30 min) are used to derive statistical analysis of the properties of IGWs with short vertical wavelengths ( ≤ 4 km) and ground-based periods longer than 4 h in the lowermost stratosphere (height range 10 to 12 km) with the help of the hodograph method. The annual change of the IGWs parameters are inspected but no pronounced year cycle is found. The year is divided into two seasons (summer and winter) based on the most prominent difference in the ratio of Coriolis parameter (f) to intrinsic frequency (ω^) distribution. Average of f∕ω^ for the winter season is 0.40 and for the summer season 0.45 and the average horizontal wavelengths are 140 and 160 km respectively. Vertical wavelengths have an average of 1.85 km through the year. For both seasons the properties of IGWs with upward and downward propagation of the energy are also derived and compared. The percentage of downward propagating waves is 10.7 and 18.4 % in the summer and winter season respectively. This seasonal change is more than the one previously reported in the studies from mid-latitudes and model-based studies. It is in agreement with the findings of past radiosonde data-based studies from the Antarctic region. In addition, using the so-called dual-beam technique, vertical momentum flux and the variance of the horizontal perturbation velocities of IGWs are examined. Tropospheric disturbances of synoptic-scale are suggested as a source of episodes of IGWs with large variance of horizontal perturbation velocities, and this is shown in a number of cases.

Measuring the variance of the velocity of atmosphere $\sigma _{turb}^2$, which is proportionally linked to the energy dissipation rate, is a common role given to MST radar. However, the spectral width $\sigma _{obs}^2$, which is directly observable with a radar, contains not only the contribution from turbulence itself but also some measurement biases. Among them, beam broadening $\sigma _{beam}^2$, which is the projected components of the mean wind velocity to the off-center sensitivity of the radar beam, is the major factor. A number of studies have been conducted to evaluate the beam broadening under an assumption that the antenna beam is conical and well defined by its beam width. However, this assumption is not applicable to nonstandard radar configurations that have distributed or asymmetric antenna array and thereby resulting asymmetric beampatterns. We present a comprehensive mathematical theory and practical algorithm to evaluate $\sigma _{beam}^2$ that is applicable to any antenna structure.

Typical studies on the history of science, or particularly of biology, have been focused on a particular scientist or book, but this selection has a risk of being arbitrary. To find a more objective way of studying history of biology, we applied a statistical method. First, we downloaded from the PubMed database all available titles and abstracts of 934,807 articles in 32 selected journals from 1965 to 2014, and extracted most frequently used 322 terms by text mining. Clustering of these terms according to the annual frequency of usage resulted in three main clusters: Cluster 1 represented terms that were no longer used frequently, Cluster 3 included terms that became abundantly used recently, and Cluster 2 contained terms constantly used. Three phases were delineated in the history of biology over the past 50 years, with transitions in 1987 and 1997. In contrast with our tacit understanding that “function” is a key notion in biological thinking, the results suggest that function-oriented discourses are a new habit of biologists in the genomic era after 1997, in which biological researches focus on identifying a link between a molecule or a structure with its function. We hypothesize that, in spite of repeated warnings, function-related discourses have a teleological connotation, which is easily misunderstood by general audience and, with emphatic expressions such as “important” and “essential”, fit to the need for justification of researches as part of researcher's responsibility for public funding.

The climatology of residual mean circulation, which is a main component of Brewer-Dobson circulation, and the potential contribution of gravity waves (GWs) are examined for the annual mean state and for each season based on the transformed-Eulerian mean zonal momentum equation using modern four reanalysis data, which allows us to examine the whole stratosphere. First, the potential contribution of Rossby waves (RWs) to residual mean circulation is estimated from Eliassen-Palm flux divergence. The rest of residual-mean circulation, from which the potential RW contribution and zonal mean zonal wind tendency are subtracted, is regarded as the potential GW contribution. These potential wave contributions are exact contributions for the annual mean state and give good approximates for solstitial seasons. The GWs contribute to drive not only the summer hemispheric part of the winter deep branch and low-latitude part of shallow branches, as indicated by previous studies, but also cause a higher-latitude extension of the deep circulation in all seasons except for summer. This GW contribution is essential to determine the location of the turn-around latitude. The autumn circulation is stronger and wider than that of spring in the equinoctial seasons, regardless of almost symmetric RW and GW contributions around the equator. This asymmetry is attributable to the existence of the spring-to-autumn pole circulation corresponding to the angular momentum transport associated with seasonal variation due to the radiative process. The potential GW contribution is larger in September-to-November than in March-to-May in both hemispheres. The upward mass flux is maximized in the boreal winter in the lower stratosphere, while it exhibits semi-annual variation in the upper stratosphere. The GW contribution to the annual mean upward mass flux is in a range of 10–30 %, depending on the reanalysis data. The boreal winter maximum in the lower stratosphere is attributable to stronger RW activity in both hemispheres than in the austral winter.

Bioconvection is a convective flow found in a suspension of motile cells that swim against gravity, and is a primitive form of order formation of cells, which has been studied both experimentally and theoretically. We formulate here an inverted bioconvection occurring in a suspension of phototactic cells in a high-density medium, which is illuminated from the bottom. We used a highly phototactic strain 137c of Chlamydomonas reinhardtii in the experiments. Using a custom-made lateral microscope, we observed a close view of cellular dynamics in the initiation of inverted bioconvection. In conventional bioconvection, convective flows of cells are formed spontaneously with or without formation of the surface cell layer. In inverted convection, a crowded cell layer was initially formed at the bottom, which was a prerequisite for the subsequent emergence of plumes, namely, floating populations of cells. The plume formation was a result of neither uneven initial cell density nor unequal light intensity. Based on detailed analysis of individual cells, we constructed a model of inverted bioconvection, in which each cell experiences a transition between two modes of movement: phototactically swimming cell and non-motile cell aggregate. A simulation using the CompuCell3D software reproduced basic behaviors of the plume formation. The modal transition has not been a subject of basic studies, but provides an interesting target of study of cell-to-cell interactions.

This study shows that gravity wave (GW) forcing (GWF) plays a crucial role in the barotropic/baroclinic instability that is frequently observed in the mesosphere and considered an origin of planetary waves (PWs) such as quasi-2-day and quasi-4-day waves. Simulation data from a GW-resolving general circulation model were analyzed, focusing on the winter Northern Hemisphere where PWs are active. The unstable field is characterized by a significant potential vorticity (PV) maximum with an anomalous latitudinal gradient at higher latitudes that suddenly appears in the midlatitudes of the upper mesosphere. This PV maximum is attributed to an enhanced static stability that develops through the following two processes: 1) strong PWs from the troposphere break in the middle stratosphere, causing a poleward and downward shift of the westerly jet to higher latitudes, and 2) strong GWF located above the jet simultaneously shifts and forms an upwelling in the midlatitudes, causing a significant increase in . An interesting feature is that the PV maximum is not zonally uniform but is observed only at longitudes with strong GWF. This longitudinally dependent GWF can be explained by selective filtering in the stratospheric mean flow modified by strong PWs. In the upper mesosphere, the Eliassen–Palm flux divergence by PWs has a characteristic structure, which is positive poleward and negative equatorward of the enhanced PV maximum, attributable to eastward- and westward-propagating PWs, respectively. This fact suggests that the barotropic/baroclinic instability is eliminated by simultaneous generation of eastward and westward PWs causing PV flux divergence.

Recent advances in human induced pluripotent stem cells (hiPSCs) offer new possibilities for biomedical research and clinical applications. Differentiated neurons from hiPSCs are expected to be useful for developing novel methods of treatment for various neurological diseases. However, the detailed process of functional maturation of hiPSC‐derived neurons (hiPS neurons) remains poorly understood. This study analyzes development of hiPS neurons, focusing specifically on early developmental stages through 48 hr after cell seeding; development was compared with that of primary cultured neurons derived from the rat hippocampus. At 5 hr after cell seeding, neurite formation occurs in a similar manner in both neuronal populations. However, very few neurons with axonal polarization were observed in the hiPS neurons even after 48 hr, indicating that hiPS neurons differentiate more slowly than rat neurons. We further investigated the elongation speed of axons and found that hiPS neuronal axons were slower. In addition, we characterized the growth cones. The localization patterns of skeletal proteins F‐actin, microtubule, and drebrin were similar to those of rat neurons, and actin depolymerization by cytochalasin D induced similar changes in cytoskeletal distribution in the growth cones between hiPS neurons and rat neurons. These results indicate that, during the very early developmental stage, hiPS neurons develop comparably to rat hippocampal neurons with regard to axonal differentiation, but the growth of axons is slower. © 2015 The Authors. Journal of Neuroscience Research Published by Wiley Periodicals, Inc.

A dosimetry system for computed tomography (CT) examinations, named WAZA-ARI, is being developed to accurately assess radiation doses to patients in Japan. For dose calculations in WAZA-ARI, organ doses were numerically analyzed using average adult Japanese male (JM) and female (JF) phantoms with the Particle and Heavy Ion Transport code System (PHITS). Experimental studies clarified the photon energy distribution of emitted photons and dose profiles on the table for some multi-detector row CT (MDCT) devices. Numerical analyses using a source model in PHITS could specifically take into account emissions of x rays from the tube to the table with attenuation of photons through a beam-shaping filter for each MDCT device based on the experiment results. The source model was validated by measuring the CT dose index (CTDI). Numerical analyses with PHITS revealed a concordance of organ doses with body sizes of the JM and JF phantoms. The organ doses in the JM phantoms were compared with data obtained using previously developed systems. In addition, the dose calculations in WAZA-ARI were verified with previously reported results by realistic NUBAS phantoms and radiation dose measurement using a physical Japanese model (THRA1 phantom). The results imply that numerical analyses using the Japanese phantoms and specified source models can give reasonable estimates of dose for MDCT devices for typical Japanese adults.

The contrasts in backscattered electron (BSE) images, such as topographic, channeling and mean atomic number (Z) contrasts, were investigated quantitatively from the cross section of a heat-treated steel sheet using a scanning electron microscope (SEM). High primary electron energy (EP) enhances Z contrast, whereas low EP improves channeling contrast. A high take-off angle (θ; measured from the specimen surface) also enhances Z contrast, whereas low θ improves channeling contrast. When θ becomes very low, topographic information is enhanced and superimposed on channeling contrast due to the tilt effect of BSE. The relationship of the behaviors of the Z contrast and the channeling contrast can be understood by the detection ratio of low-loss electrons (LLEs) to the inelastic BSE components emitted from the sample surface; LLEs contribute to channeling contrast, and their ratio increases with decreasing EP and θ. The systematic results obtained in this study are useful for controlling SEM conditions in order to enhance the target information in BSE images for practical materials of interest.

The aim of this study is to investigate whether ferritin can be a useful marker for the prediction of the patients with Kawasaki disease (KD) refractory to initial intravenous immunoglobulin (IVIG) therapy.This retrospective study enrolled 85 patients with KD hospitalized at Kitakyushu General Hospital during 2010-2014. These patients were divided into IVIG responders (n = 57) and non-responders (n = 28). Serum ferritin levels and the scoring systems for the prediction of non-responsiveness to initial IVIG therapy were compared between these two groups.Serum ferritin level was significantly elevated in non-responders (p = 0.010). The area under the receiver-operating characteristics curve was 0.674, and the sensitivity and specificity in more than 165 ng/ml of serum ferritin level were 70.4% and 63.2%, respectively. In two of the three prediction scoring systems, non-responders also showed significantly higher scores than responders, but many non-responders had low scores of these scoring systems. More than half of the patients with a low score of these scoring systems had high serum ferritin level (≥ 165 ng/ml).Serum ferritin level might be a useful marker for the prediction of non-responsiveness to initial IVIG therapy and could be an important complementary marker to the prediction scoring systems.

A questionnaire survey was conducted and analyzed with the analytic hierarchy process (AHP) to evaluate the suitability of carbon-fiber-reinforced plastic (CFRP) for use in automobile parts from an engineer’s viewpoint. The results indicated that carbon fiber has a higher potential for use in the framework than as an outer panel or exterior material. In addition, unidirectional and isotropic CFRPs can be used as alternatives to steel for higher-class automobiles. The critical evaluation criteria for carbon fiber are the material cost, safety, stiffness, and corrosion resistance. With the innovative carbon fiber project of the Ministry of Economy, Trade and Industry, CFRP has high potential as an alternative material for not only Class S but also Class A automobiles. In a dramatic innovation scenario with regard to the safety, stiffness, and thermal degradation of carbon fiber, CFRP was found to be a potential alternative material for more than half of the parts of Class A automobiles and several parts of Class C automobiles.

Multiple tropopauses (MTs) defined by the World Meteorological Organization are frequently detected from autumn to spring at Syowa Station (69.0°S, 39.6°E). The dynamical mechanism of MT events was examined by observations of the first mesosphere–stratosphere–troposphere (MST) radar in the Antarctic, the Program of the Antarctic Syowa MST/Incoherent Scatter (IS) Radar (PANSY), and of radiosondes on 8–11 April 2013. The MT structure above the first tropopause is composed of strong temperature fluctuations. By a detailed analysis of observed three-dimensional wind and temperature fluctuation components, it is shown that the phase and amplitude relations between these components are consistent with the theoretical characteristics of linear inertia–gravity waves (IGWs). Numerical simulations were performed by using a nonhydrostatic model. The simulated MT structures and IGW parameters agree well with the observation. In the analysis using the numerical simulation data, it is seen that IGWs were generated around 65°S, 15°E and around 70°S, 15°E, propagated eastward, and reached the region above Syowa Station when the MT event was observed. These IGWs were likely radiated spontaneously from the upper-tropospheric flow around 65°S, 15°E and were forced by strong southerly surface winds over steep topography (70°S, 15°E). The MT occurrence is attributable to strong IGWs and the low mean static stability in the polar winter lower stratosphere. It is also shown that nonorographic gravity waves associated with the tropopause folding event contribute to 40% of the momentum fluxes, as shown by a gravity wave–resolving general circulation model in the lower stratosphere around 65°S. This result indicates that they are one of the key components for solving the cold-bias problem found in most climate models.

Characteristically strong vertical wind disturbances (VWDs) with magnitudes larger than 1 m s−1 were observed in the Antarctic troposphere using a new mesosphere–stratosphere–troposphere (MST) radar called the Program of the Antarctic Syowa MST/incoherent scatter (IS) Radar (PANSY) during 15–19 June 2012 at Syowa Station (69.0°S, 39.6°E). In the same period, two synoptic-scale cyclones approached Syowa Station and caused a strong wind event (SWE) at the surface. The VWDs observed during the SWE at Syowa Station had a nearly standing (i.e., no phase tilt with height) phase structure up to the tropopause and a power spectrum proportional to the − power of frequency. On the other hand, the observed VWDs were not associated with systematic horizontal momentum fluxes. Meteorological fields around Syowa Station during the SWE were successfully simulated using the Nonhydrostatic Icosahedral Atmospheric Model (NICAM). A strong VWD was also simulated at the model grid of 70.0°S, 40.0°E in NICAM, which had a standing phase structure similar to the observed ones. An analysis based on the Froude number showed that the simulated VWD was likely due to a hydraulic jump leeward of the coastal mountain ridge. The Scorer parameter analysis indicated that the observed VWDs at Syowa Station during 16–17 June 2012 were likely due to the hydraulic jump similar to that in NICAM. On the other hand, a possibility of lee waves was also suggested for the VWD observed on 18 June 2012.

We report height and time variations in polar mesosphere winter echoes (PMWE) based on the Program of the Antarctic Syowa mesosphere-stratosphere-troposphere/incoherent scatter (PANSY) radar observations. PMWE were identified for 110 days from March to September 2013. PMWE occurrence frequency increased abruptly in May when two solar proton events occurred. PMWE were also observed even during periods without any solar proton events, suggesting that a possible cause of the PMWE is ionization by energetic electron precipitations. The monthly mean PMWE characteristics showed that occurrence of PMWE were mainly restricted to sunlit time. This fact indicates that electrons detached from negatively charged particles play an important role. While PMWE below 72 km in altitude completely disappeared before sunset, it was detected above that altitude for a few hours even after sunset. This height dependence in the altitude range of 60–80 km can be explained qualitatively by empirical effective recombination rates.

SummaryPIWI-interacting RNAs (piRNAs) protect the genome from transposons in animal gonads. Maelstrom (Mael) is an evolutionarily conserved protein, composed of a high-mobility group (HMG) domain and a MAEL domain, and is essential for piRNA-mediated transcriptional transposon silencing in various species, such as Drosophila and mice. However, its structure and biochemical function have remained elusive. Here, we report the crystal structure of the MAEL domain from Drosophila melanogaster Mael, at 1.6 Å resolution. The structure reveals that the MAEL domain has an RNase H-like fold but lacks canonical catalytic residues conserved among RNase H-like superfamily nucleases. Our biochemical analyses reveal that the MAEL domain exhibits single-stranded RNA (ssRNA)-specific endonuclease activity. Our cell-based analyses further indicate that ssRNA cleavage activity appears dispensable for piRNA-mediated transcriptional transposon silencing in Drosophila. Our findings provide clues toward understanding the multiple roles of Mael in the piRNA pathway.

This paper investigates nonmigrating tides from the ground to the lower mesosphere using data from a high-resolution general circulation model (KANTO GCM), as well as observational data from the Sounding of the Atmosphere using Broadband Emission Radiometry instrument on board the Thermosphere-Ionosphere-Mesosphere Energetics and Dynamics satellite and from GPS radio occultation measurements obtained with the COSMIC/FORMOSAT-3 mission. We extract nonmigrating tides using a composite as a function of universal time in physical space, without performing a zonal wave number decomposition. The KANTO GCM clearly demonstrates that tropical nonmigrating tides are regarded as gravity waves excited by diabatic heating enhanced over two major continents, specifically Africa and South America. They propagate zonally, in a direction away from their sources; that is, west and eastward propagating waves are dominant on the western and eastern sides of the continents, respectively. These characteristics are observed in two satellite data sets as well, except that the amplitudes in the KANTO GCM are larger than those in the observations. Seasonal variations of nonmigrating tides are also investigated. It is suggested that filtering owing to the stratopause semiannual oscillation, as well as diabatic heating in the troposphere, is important for the seasonal variations of nonmigrating tides in the stratosphere and the lower mesosphere.

The renormalization group equations (RGEs) describing spontaneous inertia–gravity wave (GW) radiation from part of a balanced flow through a quasi resonance that were derived in a companion paper by Yasuda et al. are validated through numerical simulations of the vortex dipole using the Japan Meteorological Agency nonhydrostatic model (JMA-NHM). The RGEs are integrated for two vortical flow fields: the first is the initial condition that does not contain GWs used for the JMA-NHM simulations, and the second is the simulated thirtieth-day field by the JMA-NHM. The theoretically obtained GW distributions in both RGE integrations are consistent with the numerical simulations using the JMA-NHM. This result supports the validity of the RGE theory. GW radiation in the dipole is physically interpreted either as the mountain-wave-like mechanism proposed by McIntyre or as the velocity-variation mechanism proposed by Viúdez. The shear of the large-scale flow likely determines which mechanism is dominant. In addition, the distribution of GW momentum fluxes is examined based on the JMA-NHM simulation data. The GWs propagating upward from the jet have negative momentum fluxes, while those propagating downward have positive ones. The magnitude of momentum fluxes is approximately proportional to the sixth power of the Rossby number between 0.15 and 0.4.

The extracellular L-glutamate (L-Glu) concentration is elevated in neuroinflammation, thereby causing excitotoxicity. One of the mechanisms is down-regulation of astrocyte L-Glu transporters. Some antidepressants have anti-inflammatory effects. We therefore investigated effects of various antidepressants on the down-regulation of astrocyte L-Glu transporters in the in vitro neuroinflammation model. Among these antidepressants, only paroxetine was effective. We previously demonstrated that the down-regulation of astrocyte L-Glu transporters was caused by L-Glu released from activated microglia. We here clarified that only paroxetine inhibited L-Glu release from microglia. This is the novel action of paroxetine, which may bring advantages on the therapy of neuroinflammation.

Nanomaterials have been extensively used in our daily life, and may also induce health effects and toxicity. Nanomaterials can translocate from the outside to internal organs, including the brain. For example, both nano-ZnO and nano-TiO

This paper synthesizes and summarizes atmospheric variability on time scales from seconds to decades through a phenomenological census. We focus mainly on unforced variability in the troposphere, stratosphere, and mesosphere. In addition to atmosphere-only modes, our scope also includes coupled modes, in which the atmosphere interacts with the other components of the Earth system, such as the ocean, hydrosphere, and cryosphere. The topics covered include turbulence on time scales of seconds and minutes, gravity waves on time scales of hours, weather systems on time scales of days, atmospheric blocking on time scales of weeks, the Madden–Julian Oscillation on time scales of months, the Quasi-Biennial Oscillation and El Niño–Southern Oscillation on time scales of years, and the North Atlantic, Arctic, Antarctic, Pacific Decadal, and Atlantic Multidecadal Oscillations on time scales of decades. The paper serves as an introduction to a special collection of Geophysical Research Letters on atmospheric variability. We hope that both this paper and the collection will serve as a useful resource for the atmospheric science community and will act as inspiration for setting future research directions.

15th MST Radar WorkshopSession M6: Middle atmosphere dynamics and structureMay 31 (Wed), NIPR Auditorium