201. Synthesis and biological evaluation of fluoro-substituted 3,4-dihydroquinazoline derivatives for cytotoxic and analgesic effects.
- Author
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Kim, Jin Han, Jeong, Hui Rak, Jung, Da Woon, Yoon, Hong Bin, Kim, Sun Young, Kim, Hyoung Ja, Lee, Kyung-Tae, Gadotti, Vinicius M., Huang, Junting, Zhang, Fang-Xiong, Zamponi, Gerald W., and Lee, Jae Yeol
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QUINAZOLINE , *HETEROCYCLIC compounds synthesis , *ANTINEOPLASTIC agents , *CALCIUM channels , *BIOISOSTERES , *NON-small-cell lung carcinoma , *LABORATORY rats - Abstract
As a bioisosteric strategy to overcome the poor metabolic stability of lead compound KYS05090S, a series of new fluoro-substituted 3,4-dihydroquinazoline derivatives was prepared and evaluated for T-type calcium channel (Ca v 3.2) block, cytotoxic effects and liver microsomal stability. Among them, compound 8h (KCP10068F) containing 4-fluorobenzyl amide and 4-cyclohexylphenyl ring potently blocked Ca v 3.2 currents (>90% inhibition) at 10 μM concentration and exhibited cytotoxic effect (IC 50 = 5.9 μM) in A549 non-small cell lung cancer cells that was comparable to KYS05090S. Furthermore, 8h showed approximately a 2-fold increase in liver metabolic stability in rat and human species compared to KYS05090S. Based on these overall results, 8h (KCP10068F) may therefore represent a good backup compound for KYS05090S for further biological investigations as novel cytotoxic agent. In addition, compound 8g (KCP10067F) was found to partially protect from inflammatory pain via a blockade of Ca v 3.2 channels. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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