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213. How to Grow Minnows

Author

Carlander, K. D., primary, Allan, Philip F., additional, Prather, E. E., additional, Fielding, J. R., additional, Johnson, M. C., additional, and Swingle, H. S., additional

Published
1953
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237. An Endophytic Fungus and Resistance to Sod Webworms: Association in Lolium Perenne L.

Author

Funk, C. R., Halisky, P. M., Johnson, M. C., Siegel, M. R., Stewart, A. V., Ahmad, S., Hurley, R. H., and Harvey, I. C.

Abstract

Sod webworm (Crambus spp.) susceptible and resistant cultivars, selections, and single–plant progenies of perennial rye–grass (Lolium perenne L.) were analyzed for the presence of an endophytic fungus. This was done using both the ELISA technique and microscopic examination of remnant seed samples. All entries resistant to sod webworms in field trials contained high levels of fungal endophyte, whereas susceptible entries did not contain the endophyte. The significance of this endophyte–enhanced resistance to insects in the breeding and maintenance of turfgrass and forage cultivars is discussed.

Published
1983
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240. Understanding Structure-Function Questions and Crystallization Behavior of Eukaryotic Membrane Proteins by Combining Microscopies.

Author

Johnson, M. C., Mari, S., Rudolph, F., Zhao, G., Haase, W., Kanaoka, Y., Stafford, D., Müller, D., and Schmidt-Krey, I.

Subjects
MEMBRANE proteins
Abstract

Extended abstract of a paper presented at Microscopy and Microanalysis 2010 in Portland, Oregon, USA, August 1 – August 5, 2010. [ABSTRACT FROM PUBLISHER]

Published
2010
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242. Association between MMP1 and MMP9 activities and ICAM1 cleavage induced by tumor necrosis factor in stromal cell cultures from eutopic endometria of women with endometriosis.

Author

Pino, M., Galleguillos, C., Torres, M., Sovino, H., Fuentes, A., Boric, M. A., and Johnson, M. C.

Subjects
IMMUNE system, ENDOMETRIOSIS, CELL culture, TUMOR necrosis factors, GYNECOLOGIC pathology
Abstract

Endometriosis is a benign gynecological pathology in which immune system deregulation may play a role in its initiation and progression. In endometriotic lesions, intercellular adhesion molecule-1 (ICAM1) is released from the cell membrane by proteolytic cleavage of its extracellular domain, a process that coincides with increased expression and proteolytic activity of metalloproteinases such as MMP1 and MMP9. The objective of our study was to investigate the association between MMP1 and MMP9 activities and ICAM1 cleavage mediated by tumor necrosis factor (TNF) in eutopic endometrial stromal cells from women with and without (control) endometriosis during culture. The RNA was evaluated by RT-PCR, and the protein was determined by western blot (ICAM1, MMP1), casein or gelatin zymographies (secreted active MMP1 or MMP9 respectively), ELISA (soluble ICAM1 (sICAM1)), and fluorescence assay (secreted active MMP1). Under basal conditions, proMMP9 dimer and MMP9 were higher in endometriosis cell cultures. In stromal cultures derived from control women and those with endometriosis, TNF augmented the intracellular proMMP1 (1.2-fold in control stromal cells) and ICAM1 (1.4- and 1.9-fold), greatly increased MMP1 and proMMP9 levels, and the sICAM1 concentration (2.3- and 4.3-fold) in their media compared with basal levels. The combination of TNF and MMP9 increased the sICAM1 concentration 14-fold in the endometriosis cell media, whereas GM6001 inhibited the stimulatory effect of TNF in both cell cultures. The deregulation of MMP9, and the TNF participation in the MMP1 and proMMP9 secretions, in the MMP9 expression and in the expression and cleavage of ICAM1 may contribute to the pathophysiology of this disease. [ABSTRACT FROM AUTHOR]

Published
2009
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243. WASP STING OF THE CORNEA

Author

Johnson, M. C.

Abstract

Wasp stings of the cornea are not of common occurrence. The American literature on this subject is relatively meager. The article by Yoshida,1 translated by S. R. Gifford, is perhaps the most complete. The foreign literature contains more reference to this subject, though it is by no means voluminous.Lewin and Guillery2 and Zander and Geissler3 state that wasp stings of the cornea are more dangerous than those of the bee. Cases of death within ten minutes following the sting of the wasp have been reported. Kraupa4 concludes that there must be a chemical difference in the toxins formed by the two insects. Bee stings of the cornea, with case reports, have recently been reviewed by Young.5The changes described in wasp stings of the cornea consist in the following points: There is a loss of corneal epithelium at the point of injury, surrounded by

Published
1932
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