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To investigate an air distribution system with the potential to provide a comfortable thermal environment and efficient ventilation performance in heating mode, this study compared the numerical results of the RNG k-[Formula: see text] model, realizable k-[Formula: see text] model and SST k-ω model with experimental data obtained from a typical office with wall confluent jets ventilation (WCJV) for heating. The SST k-ω model was chosen for numerical study of WCJV. The findings showed that the WCJV performed better than mixing ventilation (MV) in the heating mode because WCJV could entrain less ambient air and maintain the jets’ momentum and energy to reach the occupied zone. The effects of supply air temperature difference (in the range of 2.00°C to 7.79°C) and supply air velocity (in the range of 2.20 m/s to 8.50 m/s) on the jet velocity profiles and ventilation performance of WCJV were also quantified. The results showed that the jet velocity profile was more sensitive to the supply air velocity than to the supply air temperature difference. Accordingly, reducing the supply air velocity might result in poorer thermal environment and air quality in the occupied zone because the warm air jets cannot spread out over the floor area. The results can be helpful in understanding WCJV used for ventilation under heating mode.

We described a simple method for detection of Fe on a thread-based analytical device. Fe reacts with 1,10-phenanthroline to form a colored band on a cotton thread; the length was measured to quantify the contents of Fe using a ruler. This method has the advantages of easy operation, low cost, easy fabrication, and reduced sample/reagent consumption. Furthermore, this method is free of any electronic equipment such as camera and digital scanner. These features make this method suitable as a laboratory experiment in universities and colleges. This laboratory experiment is designed as one of selected experiments of the Comprehensive Chemistry Experiment course for the students majoring in chemistry, for the sixth and seventh semester. The students enjoyed fabricating the thread-based analytical device and used it to detect the contents in unknown samples. This laboratory experiment could be completed within 3 h.

As a significant biomarker of prion diseases, ultrasensitive assay of infectious isoform of prion (PrPSc) is highly desirable for early diagnostics of prion diseases. Herein, taking normal cellular form of prion (PrPC) as a model owing to a high risk of pathogenicity of PrPSc, a new photoelectrochemical immunosensor has been developed based on hemin-induced switching of photocurrent direction. In the presence of PrPC, nitrogen-doped porous carbon-hemin polyhedra labeled with secondary antibody were introduced onto the CdS-chitosan (CS) nanoparticles-modified indium–tin oxide (ITO) electrode via the antigen–antibody specific recognition. Because of the matched energy level between CdS and hemin, the high-efficiency switch of photocurrent direction of the ITO/CdS-CS photoelectrode from anodic to cathodic photocurrent was observed even at very low concentration (0.4 aM) of PrPC. Through changing the specific antibody, this method can be easily expanded to PrPSc assay. Such low detectable limit is very useful in the early diagnosis and screening of prion diseases. The developed method has also promising applications in bioanalysis, disease diagnostics, and clinical biomedicine.

N-enriched porous carbon materials represent a kind of effective electrocatalysts for oxygen reduction reaction (ORR), which have received extensive attention owing to their low-cost, advantageous catalytic activity and stability. However, exploring non-noble-metal catalysts for replacing high-cost notable metal catalysts remains a major challenge. Herein, iron and sulfur co-doped N-enriched hierarchical porous carbon polyhedron (NC) derived from metal-organic framework (Fe/S-NC) was successfully prepared by a facile strategy, including a direct pyrolyzation of zeolitic imidazolium framework (ZIF-8) and a further pyrolysis of the impregnated NC with Fe(SCN)3 solution. Electron microscopic studies showed that Fe/S co-doping has not changed the polyhedron morphology of NC. The resulted Fe/S-NC demonstrated obviously enhanced ORR activity, excellent durability and methanol tolerance in both alkaline and acidic media. The half-wave potential of the Fe/S-NC exhibited 32 mV positive shift in 0.1 M KOH, and only 25 mV negative shift in 0.5 M H2SO4, respectively, as compared to that of the commercial Pt/C (20% Pt loading) catalyst. The superior performance is attributable to the combined roles of the unique hierarchical porous structure of NC and the Fe/S co-doping by endowing Fe/S-NC with great specific surface area, rich active sites, excellent conductivity and synergistic effect between Fe-Nx-C active sites and a thiophene-like structure (C-S-C).

Here, based on a newly developed “Z-scheme” CdSe QD/afGQD heterojunction and multiple signal amplification strategies, a sensitive photoelectrochemical methyltransferase activity assay was carried out and a low detection limit of 0.046 U mL−1 was obtained.

The development of non-platinum catalysts for the oxygen reduction reaction (ORR) to substitute state-of-the-art but expensive Pt catalysts is essential for the development and practical application of fuel cells. Here, combining the self-assembly and coordination interaction between graphene oxide (GO) with g-C3N4 and iron phthalocyanine (FePc), FeNx atom clusters supported on three-dimensional porous nitrogen-enriched graphene carbon aerogels were successfully prepared by one facile solution method. The obtained FeNx atom cluster catalyst (FeNx-CN/g-GEL) exhibited superb catalytic performance (E1/2 = 0.90 V) toward the ORR, which is superior to that of commercial Pt/C (E1/2 = 0.835 V) and the currently reported non-noble nanocatalysts. Additionally, the catalyst displayed a high metal utilization rate with a mass specific activity of 840 mA mgFe−1 at 0.80 V, excellent long-term stability with only a little decline after the 28 000 s test and remarkable tolerance to methanol and SCN−.

Dietary polyphenols, a natural component in many kinds of foods such as fruits and vegetables, play essential roles in a wide range of plant functions. Importantly, the discovery of the functions of polyphenols including anti-oxidant, anti-carcinogenic and anti-inflammatory has been appealing to researchers' attentions. Dietary polyphenols have shown protective effects on chronic degenerative diseases (CDD) such as cardiovascular diseases, cancers, and neurodegenerative diseases by regulating gene expression. Dietary polyphenols also affect the composition and activity of gut microbiota, in reverse, gut microbiota influences the bioavailability and physiological activity of dietary polyphenols. However, not all kinds of dietary polyphenols are beneficial for human health. The potential deleterious effects of several dietary polyphenols have been reported by inducing DNA damage and gene mutants. This review summarizes the potential therapeutic effects of dietary polyphenols on chronic degeneration diseases, the polyphenols-gut microbiota interactions, and the potential dangers of individual dietary polyphenols on human health.

This mixed‐method study examines whether and how the International Accounting Standards Board (IASB) was influenced by interest groups during the development of the expected credit loss (ECL) model for IFRS 9 Financial Instruments. Content analysis of 327 comment letters revealed that the IASB was influenced. However, Fisher's exact test and chi‐square goodness‐of‐fit test showed that, to a greater extent, the influence was not significant. Furthermore, qualitative analyses of the arguments put forward by interest groups showed that as a result of interest groups’ inputs, accounting requirements for the ECL model were made more operational, less complex and potentially productive of more comparable financial information.

Indoles are an important class of molecules. This paper describes an efficient palladium-catalyzed synthesis of indoles from 2-iodostyrenes and di-t-butyldiaziridinone with a simultaneous installation of two C-N bonds. The reaction process likely proceeds through the oxidative insertion of Pd to aryl iodide and subsequent vinyl C-H activation to from a pallada(II)cycle intermediate, which is bisaminated by di-t-butyldiaziridinone to give the indole product.

A chelating fabric was prepared by graft polymerization of glycidyl methacrylate (GMA) onto a nonwoven fabric, followed by attachment reaction of N-methyl-D-glucamine (NMDG) using an organic solvent-free process. The graft polymerization was performed by immersing the gamma-ray pre-irradiated fabric into the GMA emulsion, while the attachment reaction was carried out by immersing the grafted fabric in the NMDG aqueous solution. The chelating capacity of the chelating fabric prepared by reaction in the NMDG aqueous solution without any additives reached 1.74 mmol/g, which further increased to above 2.0 mmol/g when surfactant and acid catalyst were added in the solution. The boron chelation of the chelating fabric was evaluated in a batch mode. Fourier transform infrared spectrophotometer (FTIR) was used to characterize the fabrics. The chelating fabric can quickly chelate boron from water to form a boron ester, and a high boron chelating ability close to 18.3 mg/g was achieved in the concentrated boron solution. The chelated boron can be eluted completely by HCl solution. The regeneration and stability of the chelating fabric were tested by 10 cycles of the chelation-elution operations. Considering the organic solvent-free preparation process and the high boron chelating performance, the chelating fabric is promising for the boron removal from water.

Heterobifunctional compounds that direct the ubiquitination of intracellular proteins in a targeted manner via co-opted ubiquitin ligases have enormous potential to transform the field of medicinal chemistry. These chimeric molecules, often termed proteolysis-targeting chimeras (PROTACs) in the chemical literature, enable the controlled degradation of specific proteins via their direction to the cellular proteasome. In this report, we describe the second phase of our research focused on exploring antibody-drug conjugates (ADCs), which incorporate BRD4-targeting chimeric degrader entities. We employ a new BRD4-binding fragment in the construction of the chimeric ADC payloads that is significantly more potent than the corresponding entity utilized in our initial studies. The resulting BRD4-degrader antibody conjugates exhibit potent and antigen-dependent BRD4 degradation and antiproliferation activities in cell-based experiments. Multiple ADCs bearing chimeric BRD4-degrader payloads also exhibit strong, antigen-dependent antitumor efficacy in mouse xenograft assessments that employ several different tumor models.

Background: In recent years, targeted therapy combined with traditional chemoradiotherapy and surgery has brought new opportunities for cancer treatment. However, the complex characteristics of cancer, such as heterogeneity and diversity, limit the clinical success of targeted drugs. Discovering of new cancer targets and deepening the understanding of their functional mechanisms will bring additional promising application prospects for the research and development of personalized cancer-targeted drugs. Objective: This study aimed to summarize the role of the Rho GTPase activating protein 9 (ARHGAP9) gene in tumorigenesis and development to discover therapeutic targets for cancer in the future. Methods: For this review, we collected patents from the databases of Espacenet and WIPO and articles from PubMed that were related to the ARHGAP9 gene. Results: Genetic/epigenetic variations and abnormal expression of the ARHGAP9 gene are closely associated with a variety of diseases, including cancer. ARHGAP9 can inactivate Rho GTPases by hydrolyzing GTP into GDP and regulate cancer cellular events, including proliferation, differentiation, apoptosis, migration and invasion, by inhibiting JNK/ERK/p38 and PI3K/AKT signaling pathways. In addition to reviewing these mechanisms, we assessed various patents on ARHGAP9 to determine whether ARHGAP9 might be used as a predictive biomarker for diagnosis/prognosis evaluation and a druggable target for cancer treatment. Conclusion: In this review, the current knowledge of ARHGAP9 in cancer is summarized with an emphasis on its molecular function, regulatory mechanism and disease implications. Its characterization is crucial to understanding its important roles during different stages of cancer progression and therapy as a predictive biomarker and/or target.

BACKGROUNDContrast-induced encephalopathy is a rare complication of cerebral angiography with only few cases reported to date. This paper reports on contrast-induced encephalopathy mimicking meningoencephalitis following cerebral angiography with iopromide, a subhypertonic nonionic contrast agent.OBSERVATIONSA 50-year-old woman underwent cerebral angiography for assessment of recurrent nasopharyngeal carcinoma with invasion of internal carotid artery. The patient experienced symptoms including a disturbance of consciousness, seizures, frequent blinking, and stiffness in the extremities immediately after angiography of the left common carotid artery using iopromide (4 ml/s, total 6 ml). Computed tomography scans of the brain showed no obvious abnormalities, whereas brain magnetic resonance imaging showed swelling of the left cerebral cortex without signs of ischemia or hemorrhage. The patient was treated with intravenous rehydration, mannitol dehydration, and other supportive treatment. With this treatment, neurological status progressively improved, with complete resolution of symptoms at day 10.LESSONSThis observation highlights that even a small dose of subhypertonic nonionic contrast agent can rapidly induce contrast encephalopathy.

With the development of marine engineering, the noise of underwater motors has received more and more attention. Aiming at the problem of underwater acoustic radiation caused by electromagnetic force waves of underwater motors through the vibration of the casing, this paper first analyzes the different causes of the vibration caused by the excited shell in water and air; secondly, the same sound pressure and the same sound power are derived respectively In the three cases with the same excitation force, the difference of the sound pressure level of the shell in air and water, focusing on the effect of the underwater acoustic impedance on the difference of the sound pressure level un-der the same excitation force. Finally, taking a 24-pole 36-slot permanent magnet synchronous motor as an ex-ample, the analytical method and finite element simulation are used to analyze the sound pressure level difference of the motor under different media to verify the correctness of the analytical method. The underwater motor is directly analyzed by the noise in the air Provide a reference for predicting underwater noise.

In order to utilize the low-concentration gas directly discharged into the atmosphere from 1.1% to 1.50% in coal mine production, the heat storage and oxidation equipment of gas was improved, and the heat storage and safety of gas under high-temperature environment were tested. The experimental results show that the regenerative oxidation system could provide a high-temperature oxidation environment of 1000°C for gas oxidation after changing the heating mode and enhancing the sealing property. The pressure curves of air and gas in the burner were similar. With the increase of gas concentration, the pressure difference between inlet and outlet tended to increase linearly with a minimum pressure differential of 4 kPa (air) and a maximum pressure differential of 11 kPa (1.50% gas). The internal pressure was relatively stable without an instantaneous pressure surge or explosion. The result of this study provides a reference for further research on the low-concentration gas regeneration oxidation unit at high temperatures.

Chaetoglobosin G (CG) is a fungal secondary metabolite and shows anti-tumor effects. However, the mechanisms behind the anti-tumor effect is still unclear. In this study, we evaluated the anti-proliferation effect of CG on human NSCLC A549 cells and explored the underlying mechanisms. The anti-proliferation effect of CG on A549 cells was evaluated by MTT. The targets of CG were screened through transcriptome sequencing. A flow cytometer was used to detect cell cycle and apoptosis. Western blotting was used to analyze apoptosis, cell cycle and autophagy related protein expression. Our results showed that CG had a dose-dependent inhibitory effect on proliferation of A549 cells. Transcriptome sequencing analysis found that CG obviously induced cell cycle arrest. Flow cytometry analysis and western blot showed that CG induced G2/M arrest with p21 protein upregulation and cyclinB1 protein downregulation. Western blot analysis also indicated that p-EGFR, EGFR, p-MEk and p-ERK protein expressions decreased and autophagy protein LC3II expression increased, indicating that CG can promote autophagy through EGFR/MEK/ERK/LC3 pathway. Moreover, CG can induce apoptosis with bcl-2 protein decrease. In conclusion, this study indicated that CG obviously inhibited A549 cell proliferation, and its mechanism may induce autophagy of A549 cells through EGFR/MEK/ERK/LC3 pathway to upregulate the expression of P21, thus lead to G2/M phase arrest to exert an anti-tumor role.

The moving magnet voice coil actuator (MMVCA) is a promising choice for the long stroke nanopositioning stage with the advantage of low moving mass. However, the hysteresis observed in MMVCA limits further improvement on tracking performance. The hysteresis is cascading with the linear dynamic of the positioning stage, which makes common hysteresis identification inapplicable. In this paper, the cause and influence of hysteresis in MMVCA are analyzed, which reveal that the magnetic hysteresis leads to a hysteresis of force and causes motion accuracy to degrade. A modified rate-dependent Prandtl–Ishlinskii (P–I) model is proposed to describe the hysteresis in MMVCA. The decoupled method is implemented to identify the parameters of the linear dynamic model and nonlinear hysteresis model. The experimental results validate the feasibility of the proposed P–I model. Based on the hysteresis compensation, the peak-to-peak tracking errors are reduced by 30% and the root-mean-square (rms) tracking errors are decreased by 41% on average for the trajectories with amplitudes from 1 to 3 mm and frequencies from 1 to 5 Hz.

A modified Gilbert down-conversion mixer for 77-GHz automotive radar application is presented in this paper. By replacing the resistive loads with active loads composed of resistors and PMOSs, the output DC voltage becomes stabler, and the suppression of even-order components is improved. Fabricated in 40-nm CMOS technology, the proposed mixer occupies a core chip area of $0.25 \times 0.26$ mm2. The DC power consumption is only 1.5 mW with a 1-V supply. The measured maximum voltage conversion gain is 4.8 dB at LO frequency of 78.5 GHz and IF frequency of 20 MHz. The input 1-dB compression point is 0.8 dBm. Measured results also show that the reflection coefficient of the RF port is less than -11 dB from 74 to 83 GHz.

A nonwoven fabric adsorbent loaded with 2-ethylhexyl hydrogen-2-ethylhexylphosphonate (EHEP) was developed for the separation and recovery of dysprosium (Dy) and neodymium (Nd) from an aqueous solution. The adsorbent was prepared by the radiation-induced graft polymerization of a methacrylate monomer with a long alkyl chain onto a nonwoven fabric and the subsequent loading of EHEP by hydrophobic interaction and chain entanglement between the alkyl chains. The adsorbent was evaluated by batch and column tests with a Dy (III) and Nd (III) aqueous solution. In the batch tests, the adsorbent showed high Dy (III) adsorptivity close to 25.0 mg/g but low Nd (III) adsorptivity below 1.0 mg/g, indicating that the adsorbent had high selective adsorption. In particular, the octadecyl methacrylate (OMA)-adsorbent showed adsorption stability in repeated tests. In the column tests, the OMA-adsorbent was also stable and showed high Dy (III) adsorptivity and high selectivity in repeated adsorption&ndash, elution circle tests. This result suggested that the OMA-adsorbent may be a promising adsorbent for the separation and recovery of Dy (III) and Nd (III) ions.

“Diversity-enhanced extracts” is an effective method of producing chemical libraries for the purpose of drug discovery. Three rare new cytochalasan derivative chaetoglobosins B1-B3 (1–3) were obtained from chemically engineered crude broth extracts of Chaetomium madrasense 375 prepared by reacting with hydrazine monohydrate and four known metabolite chaetoglobosins (4–7) were also identified from the fungus. The structures were identified by NMR and MS analysis and electronic circular dichroism simulation. In addition, the antiproliferative activities of these compounds were also evaluated, and the drug-resistant activities of cytochalasans were evaluated for the first time. Compound 6 possessed potent activity against four human cancer cells (A549, HCC827, SW620, and MDA-MB-231), and two drug-resistant HCC827 cells (Gefitinib-resistant, Osimertinib-resistant) compared with the positive controls.

Aims The imbalance of M1/M2 macrophage ratio promotes the occurrence of diabetic cardiomyopathy (DCM), but the precise mechanisms are not fully understood. The aim of this study was to investigate whether miR-471-3p/silent information regulator 1 (SIRT1) pathway is involved in the macrophage polarization during the development of DCM. Methods Immunohistochemical staining was used to detect M1 and M2 macrophages infiltration in the heart tissue. Flow cytometry was used to detect the proportion of M1 and M2 macrophages. Expression of miR-471-3p was quantified by real time quantitative-PCR. Transfection of miRNA inhibitor into RAW264.7 cells was performed to investigate the underlying mechanisms. Bioinformatics methods and western blotting were used to explore the target gene of miR-471-3p and further confirmed by dual luciferase reporter assay. Key findings We observed that M1 macrophages infiltration in the heart of tissue in DCM while M2 type was decreased. M1/M2 ratio was increased significantly in bone marrow-derived macrophages (BMDMs) from db/db mice and in RAW264.7 cells treated with advanced glycation end products (AGEs). Meanwhile, miR-471-3p was significantly upregulated in RAW264.7 cells induced by AGEs and inhibition of miR-471-3p could reduce the inflammatory polarization of macrophages. Bioinformatics analysis identified SIRT1 as a target of miR-471-3p. Both dual luciferase reporter assay and western blotting verified that miR-471-3p negatively regulated SIRT1 expression. SIRT1 agonist resveratrol could downregulate the increased proportion of M1 macrophages induced by AGEs. Conclusion Our results indicated that the development of DCM was related to AGEs-induced macrophage polarized to M1 type through a mechanism involving the miR-471-3p/SIRT1 pathway.

To investigate the effects of l-Theanine (LTA) on intestinal mucosal immunity and the regulation of short-chain fatty acid (SCFA) metabolism under dietary fiber feeding, a 28-day feeding experiment was performed in Sprague-Dawley rats. The results show that LTA increased the proportion of Prevotella, Lachnospira, and Ruminococcus while increasing the total SCFA, acetic acid, propionic acid, and butyric acid contents in the feces. LTA also increased IgA, IgE, and IgG levels in the ileum, and increased villi height and crypt depth. Moreover, LTA upregulated the mRNA and protein expression of acetyl-CoA carboxylase 1, sterol element-binding protein 1c, fatty acid synthase, and 3-hydroxy-3-methylglutaryl coenzyme A reductase in the liver, while downregulating the expression of glucose-6-phosphatase and phosphoenolpyruvate carboxykinase 1 in the colon. Our study suggests that LTA can affect intestinal mucosal immunity by regulating SCFA metabolism under dietary fiber feeding.

Coalbed gas (CBG) has been widely used as an important energy source. However, its utilization and allocation system is imperfect in mining areas. During the utilization process, a large amount of CBG is discharged into the air, causing environmental pollution. In this study, we proposed a “full spectrum-domain-time” CBG utilization model. In this model, by combining high methane concentration gas power generation, low methane concentration gas purification, and ultra-low methane concentration gas thermal storage and oxidation utilization, we were able to utilize CBG with full-spectrum of methane concentrations. In addition, by establishing CBG transportation and storage system in mining area, we were able to realize CBG supply in the entire network domain. Furthermore, based on the time series prediction algorithm, the CBG demand of different mining areas is predicted and regulatorily met by real time allocation. Through these three steps, an efficient “full spectrum-domain-time” CBG utilization system was formed and practically applied in Yangquan mining area. The application results showed that under the comprehensive control of “full spectrum-domain-time” CBG utilization model, CBG with methane concentration ≥0.2% could be used in the mining area and its utilization rate increased year by year, reaching the highest of 77.15%. Overall, our study provided a reference for the efficient CBG utilization in the mining area.

Cytotoxic pyrrolobenzodiazepine (PBD)-dimer molecules are frequently utilized as payloads for antibody-drug conjugates (ADCs), and many examples are currently in clinical development. In order to further explore this ADC payload class, the physicochemical properties of various PBD-dimer molecules were modified by the systematic introduction of acidic and basic moieties into their chemical structures. The impact of these changes on DNA binding, cell membrane permeability, and in vitro antiproliferation potency was, respectively, determined using a DNA alkylation assay, PAMPA assessments, and cell-based cytotoxicity measurements conducted with a variety of cancer lines. The modified PBD-dimer compounds were subsequently incorporated into CD22-targeting ADCs, and these entities were profiled in a variety of in vitro and in vivo experiments. The introduction of a strongly basic moiety into the PBD-dimer scaffold afforded a conjugate with dramatically worsened mouse tolerability properties relative to ADCs derived from related payloads, which lacked the basic group.

Background This study aimed to explore role of miR-646 in breast IDC. Methods miR-646, TET1, IRX1, and HIST2H2BE expression was detected by RT-qPCR and/or Western blot analysis . The methylation status of IRX1 promoter region was evaluated by methylation specific PCR. ChIP assay was used to determine the enrichment of TET1 at IRX1 promoter region. Loss- and gain-of functions were performed to determine the roles of miR-646, TET1, IRX1, and HIST2H2BE in cell proliferation , migration, invasion, and apoptosis. The tumor growth, volume, weight, and apoptosis status were measured. Results miR-646 was upregulated while TET1 was downregulated in IDC tissues. miR-646 targeted TET1. Downregulated TET1 impairs demethylation of IRX1 promoter region resulting in reduced expression of IRX1, which subsequently leads to upregulation of HIST2H2BE in IDC. Consequently, elevated HIST2H2BE promotes progression of IDC. Conclusion Our study has demonstrated that miR-646 facilitates the tumorigenesis of IDC via regulating TET1/IRX1/HIST2H2BE axis.

Background Cerebral-cardiac syndrome, newly developed cardiac damage manifestations subsequent to cerebral injuries, is a common complication of stroke and leads to increased morbidity and mortality. The current study is aimed to develop a risk prediction scale to stratify high-risk population of CCS among ischemic stroke patients. Methods The study included 410 cases from four tertiary medical centers from June 2018 to April 2019. The risk prediction model was established via logistic regression from the derivation cohort including 250 cases admitted between June 2018 and December 2018. Another 160 cases admitted from January 2019 to April 2019 were included as the validation cohort for external validation. The performance of the model was determined by the area under curve of the receiver operating characteristic curve. A rating scale was developed based on the magnitude of the logistic regression coefficient. Results The prevalence of CCS was 55.2% in our study. The predictive model derived from the derivation cohort showed good calibration by Hosmer-Lemeshow test (P = 0.492), and showed sensitivity of 0.935, specificity of 0.720, and Youden index of 0.655. The C-statistic for derivation and validation cohort were 0.888 and 0.813, respectively. Our PANSCAN score (0 to 10 points) was then established, which consists of the following independent risk factors: PT(12 s–14 s = 0; otherwise = 1), APTT(30s–45s = 0, otherwise = 1), Neutrophils(50–70% = 0; otherwise = 1), Sex(female = 1), Carotid artery stenosis(normal or mild = 0; moderate to severe = 2), Age(≥65 years = 1), NIHSS score(1 to 4 = 2; ≥5 = 3). Patients scored 3 or more points were stratified as high risk. Conclusion The risk prediction model showed satisfactory prediction effects. The PANSCAN scale provides convenient reference for preventative treatment and early management for high-risk patients. Trial registration The study was retrospectively registered in Chinese Trial Registry. The date of registration is April 17, 2019. Trial registration number: ChiCTR1900022587.

Herein, based on DNAzyme-induced disassembly of the "Z-scheme" TiO

China has the largest number of patients with dementia in the world. However, dementia in the Chinese population is still poorly understood and under-researched. Given the differences in genetic, demographic, sociocultural, lifestyle, and health profiles among Chinese and other ethnic/racial groups, it is crucial to build appropriate infrastructure for long-term longitudinal studies to advance Chinese cognitive aging and dementia research. We initiated a community-based prospective cohort-the Taizhou Imaging Study (TIS)-to accelerate the understanding of dementia and cerebrovascular diseases in Chinese. This article presents the rationale, aims, study design, and organization of TIS. In addition, we described some examples of the types of studies such a resource might support. The TIS provides a new framework for facilitating Chinese dementia research, encompassing invaluable resources including detailed epidemiological, sociocultural, neuroimaging, and omics data.

Calicheamicin antibody–drug conjugates (ADCs) are effective therapeutics for leukemias with two recently approved in the United States: Mylotarg (gemtuzumab ozogamicin) targeting CD33 for acute myeloid leukemia and Besponsa (inotuzumab ozogamicin) targeting CD22 for acute lymphocytic leukemia. Both of these calicheamicin ADCs are heterogeneous, aggregation-prone, and have a shortened half-life due to the instability of the acid-sensitive hydrazone linker in circulation. We hypothesized that we could improve upon the heterogeneity, aggregation, and circulation stability of calicheamicin ADCs by directly attaching the thiol of a reduced calicheamicin to an engineered cysteine on the antibody via a disulfide bond to generate a linkerless and traceless conjugate. We report herein that the resulting homogeneous conjugates possess minimal aggregation and display high in vivo stability with 50% of the drug remaining conjugated to the antibody after 21 days. Furthermore, these calicheamicin ADCs are highly efficacious in mouse models of both solid tumor (HER2+ breast cancer) and hematologic malignancies (CD22+ non-Hodgkin lymphoma). Safety studies in rats with this novel calicheamicin ADC revealed an increased tolerability compared with that reported for Mylotarg. Overall, we demonstrate that applying novel linker chemistry with site-specific conjugation affords an improved, next-generation calicheamicin ADC.

Nitrogen doped porous carbon nanopolyhedra (N-PCNPs) were prepared from direct carbonization of ZIF-8 nanopolyhedra. The N-PCNPs showed uniform morphology, narrow pore-size distribution centered at 3.7 nm, high surface area (2221 m2 g−1) and good electrochemical properties and were used to modify a glassy carbon electrode to electrochemically detect ascorbic acid (AA), dopamine (DA) and uric acid (UA). Compared with the bare GC electrode and reduced graphene oxide modified glassy carbon (rGO/GC) electrode, the N-PCNPs modified GC electrode (N-PCNPs/GC) was found to perform better toward electrocatalytic oxidation of AA, DA and UA. For simultaneous sensing of three analytes, three well-separated voltammetry peaks were obtained using the N-PCNPs/GC electrode in differential pulse voltammetry measurements, and the corresponding peak separations between AA and DA, DA and UA were 228 mV, 124 mV respectively. The linear response ranges for the determination of AA, DA and UA were 80–2000 μM, 0.5–30 μM and 4–50 μM, respectively, and the detection limits (S/N = 3) were 740 nM, 11 nM and 21 nM, respectively. Furthermore, the N-PCNPs/GC electrode showed good reproducibility and stability. The attractive features of N-PCNPs provided potential applications in the simultaneous determination of UA, AA and DA.

In this paper, an accumulative factor was defined to quantify the accumulative effect caused by heat storage by building envelopes in heating, ventilating, and air-conditioning (HVAC) syste...

In order to meet the application of the flexible wings of the submersible, nanocomposite hydrogels(NC gels) was prepared. Preparation of polyglycidyl methacrylate (PGMA) microspheres and correspondingly NC gels were reported. PGMA microspheres were prepared and modified by N-Methyl-D-glucamine, followed by free radical polymerization of acrylamide in the presence of microspheres to construct nanocomposite hydrogel. FT-IR, XPS and DLS verified a successful introduction of hydroxyl groups during modification. The effects of the particle size and content of the microspheres on the swelling properties of the composite hydrogels were investigated. Hydrogen bonds between hydroxyl groups and polyacrylamide molecular chains play important roles to the swelling property of NC gels. The intermolecular interaction and the crosslinking density were enhanced by the hydrogen bonds, and resultantly, swelling ratio of NC gels decreases.

To achieve efficient utilization of low-concentration mine gas, reduce resource waste, and alleviate environmental pollution, high-temperature oxidation of low-concentration gas at a concentration range of 1.00% to 1.50% that is directly discharged into the atmosphere during coal mine production was oxidized to recover heat for reuse. The gas oxidation equipment was improved for the heating process, and the safety of low-concentration gas oxidation under high-temperature environment was evaluated. Experimental results showed that the reactor could provide a 1000 ℃ high-temperature oxidation environment for gas oxidation after installing high-temperature resistant ceramics. The pressure variation curves of the reactor with air and different concentrations of gas were similar. Due to the thermal expansion, the air pressure slightly increased and then returned to normal pressure. In contrast, the low-concentration gas exhibited a stable pressure response in the high-temperature environment of 1000 ℃. The outlet pressure was significantly greater than the inlet pressure, and the pressure difference between the inlet and outlet exhibited a trend to increase with the gas concentration. The explosion limit varied with the temperature and the blend with oxidation products. The ratio of measured gas pressure to air pressure after oxidation was below the explosion criterion, indicating that the measured concentration gas is still safe after the shift of explosion limit, which provides a safe concentration range for efficient use of low-concentration gas in the future.

l-Theanine is an important component found in tea and has positive effects on nutrient absorption and transport. However, whether l-theanine can regulate glucose, lipid, and protein metabolism remains unknown. This study aims to investigate the effects of l-theanine on glucose, lipid, and protein metabolism in male Sprague-Dawley rats and characterize the underlying mechanisms. Compared to the control group, l-theanine increased the contents of hepatic and muscle glycogen, serum total protein (TP), and albumin (Alb), lowered the serum low-density lipoprotein cholesterol (LDL-C) level, decreased the activity of acetyl-CoA carboxylase (ACC), and enhanced carnitine palmitoyl transferase-1 (CPT-1) activity in the liver. Additionally, l-theanine upregulated the mRNA expression of phosphofructokinase (PFKL), CPT-1, insulin receptor (INSR), insulin receptor substrate (IRS), and liver kinase B1 (LKB1) and downregulated the mRNA expression of phosphoenolpyruvate carboxykinase 1 (PCK1), glucose-6-phosphatase catalytic subunit (G6PC), fatty acid synthase (FAS), and 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR). Moreover, l-theanine upregulated the expression of PFKL, glycogen synthase 2 (GYS2), ribosomal protein S6 (S6), INSR, IRS, and phosphatidylinositol-4,5-bisphosphate 3-kinase (PI3K) proteins; downregulated the expression of FAS, sterol regulatory element binding protein-1c (SREBP-1c), and HMGCR proteins; enhanced the phosphorylation of the mammal target of rapamycin (mTOR), ribosomal protein S6 kinase (p70S6K), protein kinase B (AKT), and AMP-activated protein kinase (AMPK); and decreased the phosphorylation of glycogen synthase kinase 3β (GSK-3β) and ACC1. Furthermore, 100 mg kg-1l-theanine was more effective at eliciting these effects than 200 and 400 mg kg-1l-theanine. In conclusion, l-theanine can regulate glucose, lipid, and protein metabolism via insulin and AMPK and their downstream signaling pathways.

Background: Bisphenol A (BPA) is a widely used industrial chemical and is associated with many diseases. Here, we investigated the relationship between serum BPA concentration and endocrine function, cytokines, and ambulatory blood pressure indicators in young patients with unexplained intracerebral hemorrhage (YUICH). Methods: 380 YUICH patients and normal control subjects (n = 380) were recruited from Changzhi People's Hospital affiliated to Shanxi Medical University and Changzhi Heping Hospital affiliated to Changzhi Medical College. The serum BPA, insulin-like growth factor-1 (IGF-1), N-terminal brain natriuretic peptide (NT pro-BNP), matrix metalloproteinase 9 (MMP-9) and TOLL-like receptor 4 ( TLR-4) level. Radioimmunoassay was used to detect the serum levels of S100B protein (S100B), interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), total testosterone (TT) and free testosterone (FT). Observe the variability of blood pressure levels through 24-hour ambulatory blood pressure monitoring, and use cerebrovascular computed tomography and magnetic resonance imaging for routine measurement. Findings: The following parameters of YUICH patients increased significantly compared with the normal control group. Serum BPA (0.85±0.15 vs 0.535±0.11 pg/ml), MMP-9 (104±10 vs 53±8.1 pg/ml), S100B (0.105±0.024 vs. 0.098±0.02μg/L), IL-1β (0.60±0.04 vs. 0.19±0.03 ng/ml), TNFα (1.29±0.13vs0.66±0.09 ng/L), TLR-4 (12.04±1.14vs 5.69±144 ), NT pro-BNP (1464±97 vs. 353), TPO-Ab (81.55±16.7 vs 8.7±2.7 IU/l). The following parameters were significantly reduced compared with the control group, IGF-1 (65.2±10.4 vs 105±9.5 ng/l), P

Chaetoglobosins belonging to cytochalasan alkaloids represent a large class of fungal secondary metabolites. To date, around 100 chaetoglobosins and their analogues have been isolated and identified over the years from a variety of fungi, mainly from the fungus Chaetomium globosum. Studies have found that chaetoglobosins possess a broad range of biological activities, including antitumor, antifungal, phytotoxic, fibrinolytic, antibacterial, nematicidal, anti-inflammatory, and anti-HIV activities. This review will comprehensively summarize the biological activities and mechanisms of action of nature-derived chaetoglobosins.

l -theanine, a non-protein-derived amino acid, is widely used as a functional ingredient in foods. Here, we evaluated the in vivo effects of l -theanine on glutamine metabolism in healthy rats and in a stressed rat model, which was established by infecting rats with enterotoxigenic Escherichia coli (E44813). The results showed that l -theanine significantly enhanced the synthesis of glutamine and stabilized the intestinal tract in healthy rats. In E44813-stressed rats, l -theanine could replace l -glutamine, thereby alleviating intestinal stress by reducing the expression of inflammatory factors, enhancing the activities of antioxidant enzymes, and regulating glutamine metabolism by modulating its synthesis. Treatment with a combination of l -theanine and l -glutamine was superior to treatment with l -glutamine alone in enhancing the activity of antioxidant enzymes, reducing the expression of inflammatory factors, and increasing the intestinal villi height and crypt depth.