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Introduction: Myopia is usually caused by excessive elongation of the eye during development. This condition is common worldwide. In clinical practice, the progression of myopia is commonly controlled through optical or drug measures, but the specific mechanisms underlying these two treatments remain unclear. To verify whether the effects of these two treatments on posterior-pole tissues are similar or different, we studied a set of common transcriptional changes in chicken models. Methods: Chicks were divided into four groups, and they were given the intervention measures of plus-lens induction, minus-lens induction, minus-lens induction with atropine injection, and minus-lens induction with saline injection. Then, the genetic changes in each tissue at the posterior pole were detected, and the results of different genes were compared. A semiquantitative real-time polymerase chain reaction method was used to further study the visually induced changes in the transcription of potential candidate genes. Results: Based on RNA sequencing (RNA-seq) analysis of the transcriptome, we identified variations between the differentially expressed transcripts in three tissues from the two treatment groups. Through Kyoto Encyclopedia of Genes and Genomes enrichment analyses, eukaryotic protein translation elongation factor 1α2 (EEF1A2) was enriched in the “leishmaniasis” pathway in the choroid and showed increased expression in both the plus-lens induction and injection atropine groups. The expression levels of selected genes verified by quantitative real-time PCR were concordant with the RNA-seq data. Conclusions: Overlapping differentially expressed mRNAs of only one-tenth could suggest a different mechanism of myopic defocus and intravitreal injection of atropine controlling myopia. EEF1A2 might play an important role in the choroid during the treatment of myopia.

Two-photon microscopy (TPM) plays an important role in the study of the changes of the two important components of neurovascular units (NVU) – neurons and blood vessels after ischemic stroke (IS). IS refers to sudden neurological dysfunction caused by focal cerebral ischemia, which is one of the leading causes of death and disability worldwide. TPM is a new and rapidly developing high-resolution real-time imaging technique used in vivo that has attracted increasing attention from scientists in the neuroscience field. Neurons and blood vessels are important components of neurovascular units, and they undergo great changes after IS to respond to and compensate for ischemic injury. Here, we introduce the characteristics and pre-imaging preparations of TPM, and review the common methods and latest progress of TPM in the neuronal and vascular research for injury and recovery of IS in recent years. With the review, we clearly recognized that the most important advantage of TPM in the study of ischemic stroke is the ability to perform chronic longitudinal imaging of different tissues at a high resolution in vivo. Finally, we discuss the limitations of TPM and the technological advances in recent years.

Discrete frequency-bin entanglement is an essential resource for applications in quantum information processing. In this Letter, we propose and demonstrate a scheme to generate discrete frequency-bin entanglement with a single piece of periodically poled lithium niobate waveguide in a modified Sagnac interferometer. Correlated two-photon states in both directions of the Sagnac interferometer are generated through cascaded second-order optical nonlinear processes. A relative phase difference between the two states is introduced by changing the polarization state of pump light, thus manipulating the two-photon state at the output of the Sagnac interferometer. The generated two-photon state is sent into a fiber polarization splitter, then a pure discrete frequency-bin entangled two-photon state is obtained by setting the pump light. The frequency entanglement property is measured by a spatial quantum beating with a visibility of $96.0 \pm 6.1\%$. The density matrix is further obtained with a fidelity of $98.0 \pm 3.0\%$ to the ideal state. Our demonstration provides a promising method for the generation of pure discrete frequency-bin entanglement at telecom band, which is desired in quantum photonics., Comment: 4 pages. 3 figures

Enterococcus faecalis is an important intestinal colonizing bacteria and can cause various tissue infections, including invasive blood infection (BI). The annual incidence of E. faecalis BI has been estimated to be ~4.5 per 100,000, with a fatality rate that can reach 20%. However, whether bacterial colonization or invasive infections are tissue based has not been thoroughly studied. In this study, we analyzed 537 clinical isolates from 7 different tissues to identify the key genomic elements that facilitate the colonization and invasive infection of E. faecalis. Comparative genomic analysis revealed that the BI E. faecalis isolates had the largest genome size but the lowest GC content, fsr quorum-sensing system genes were enriched in the BI E. faecalis, and the fsr gene cluster could enhance biofilm formation and serum resistance ability. Our findings also provide deep insight into the genomic differences between different tissue isolates, and the fsr quorum-sensing systems could be a key factor promoting E. faecalis invasion into the blood. IMPORTANCE First, we conducted an advanced study on the genomic differences between colonizing and infecting E. faecalis, which provides support and evidence for early and accurate diagnoses. Second, we discovered that fsr was significantly associated with blood infections, which also provides additional information for studies exploring the invasiveness of E. faecalis. Most importantly, we found that fsr played an important role in both biofilm formation and serum resistance ability in E. faecalis.

Herein, we re-explored α-cyano-4-hydroxycinnamic acid (CHCA) as a reactive matrix for selective and sensitive analysis of glutathione (GSH) by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). CHCA efficiently reacted with GSH, and the resulting CHCA-GSH conjugate was readily detected by MALDI-MS without interferences. The detection limit of the CHCA-GSH conjugate decreased to 200 pmol μL-1, which was 2 orders of magnitude lower than that of pure GSH.Forapplication, CHCA was successfully applied for the detection of GSH, present in HepG2 cell lysates. The results demonstrated detection advantages of simple, high-throughput, and selective and screening of GSH in biological samples by MALDI-MS.

Valvular heart disease (VHD) is a common heart disease that affects blood flow. It usually requires heart surgery. Valvular heart disease complicated with pulmonary artery hypertension (VHD-PAH) may be lethal due to heart failure that results from increased heart burden. It is important for these patients to seek early treatment in order to minimize the heart damage. However, there is no reliable diagnosis method in VHD. In this study, we found DNA methylation was increased at the promoter of BMPR2 gene in the VHD patients compared with the healthy controls. This finding was confirmed by an independent cohort study of VHD patients and healthy controls. In addition, BMPR2 mRNA levels were reduced in the plasma of the VHD patients. There is strong correlation between BMPR2 promoter DNA methylation and the severity of VHD. Indeed, we found that both BMPR2 promoter DNA methylation and BMPR2 mRNA levels in the plasma are good biomarkers of VHD by themselves, with the respective AUC value of 0.879 and 0.725, respectively. When they were used in combination, the diagnostic value was even better, with the AUC value of 0.93. Consistent with the results in the VHD patients, we observed decreased BMPR2 and increased fibrosis in the lung of a PAH model mouse. BMPR2 was also decreased in the hearts of the PAH mice, whereas BMP4 was increased. Furthermore, BMPR2 was reduced in the heart valve tissue samples of human VHD patients after valve replacement with moderate/severe PAH compared with those with mild PAH. There was also increased apoptosis in the hearts of the PAH mice. BMPR2 promoter DNA methylation and its expression appear to be good biomarkers for VHD. Our results also suggest that DNA methylation may cause PAH through deregulation of BMP signaling and increased apoptosis.

Impulsive stimulated Brillouin spectroscopy (ISBS) plays a critical role in investigating mechanical properties thanks to its fast measurement rate. However, traditional Fourier transform-based data processing cannot decipher measured data sensitively because of its incompetence in dealing with low signal-to-noise ratio (SNR) signals caused by a short exposure time and weak signals in a multi-peak spectrum. Here, we propose an adaptive noise-suppression Matrix Pencil method for heterodyne ISBS as an alternative spectral analysis technique, speeding up the measurement regardless of the low SNR and enhancing the sensitivity of multi-component viscoelastic identification. The algorithm maintains accuracy of 0.005% for methanol sound speed even when the SNR drops 33 dB and the exposure time is reduced to 0.4 ms. Moreover, it proves to extract a weak component that accounts for 6% from a polymer mixture, which is inaccessible for the traditional method. With its outstanding ability to sensitively decipher weak signals without spectral a priori information and regardless of low SNRs or concentrations, this method offers a fresh perspective for ISBS on fast viscoelasticity measurements and multi-component identifications.

The blue-light hazard (BLH) has raised concerns with the increasing applications of white light-emitting diodes (LEDs). Many researchers believed that the shorter wavelength or more light components generally resulted in more severe retinal damage. In this study, based on the conventional phosphor-coated white LED, we added azure (484 nm), cyan (511 nm), and red (664 nm) light to fabricate the low-hazard light source. The low-hazard light sources and conventional white LED illuminated 68 Sprague-Dawley (SD) rats for 7 days. Before and after light exposure, we measured the retinal function, thickness of retinal layers, and fundus photographs. The expression levels of autophagy-related proteins and the activities of oxidation-related biochemical indicators were also measured to investigate the mechanisms of damaging or protecting the retina. With the same correlated color temperature (CCT), the low-hazard light source results in significantly less damage on the retinal function and photoreceptors, even if it has two times illuminance and blue-light hazard-weighted irradiance ([Formula: see text]) than conventional white LED. The results illustrated that [Formula: see text] proposed by IEC 62471 could not exactly evaluate the light damage on rats' retinas. We also figured out that more light components could result in less light damage, which provided evidence for the photobiomodulation (PBM) and spectral opponency on light damage.

Background By establishing a finite element model of unilateral biportal endoscopic lumbar interbody fusion (UBE-LIF), the biomechanical characteristics of UBE-LIF technology were analysed and evaluated. The risk of cage subsidence was also evaluated. Methods The finite element model of the L4-L5 vertebral body was constructed based on CT data from healthy adult male volunteers. According to the UBE-LIF surgical method, the fusio model with different cage heights of 8 mm, 10 mm and 12 mm was successively constructed. The flexion, extension, right lateral bend, left lateral bend, right axial rotation, and left axial rotation motions were simulated in 6 models with different bone conditions on the upper surface of L4 with 500 N followed load and 10 Nm torsional torque. The range of motion(ROM), Pedicle screw-rod system stress and endplate stress of each model under different working conditions were observed and analysed. Results The ROM of the 12mm model was the lowest, simultaneously, the maximum stress of the pedicle screw-rod system of the 12mm model was the lowest. However, the 12mm model has the largest endplate stress. The maximum stress of the L4 inferior endplate was greater than that of the L5 superior endplate in the UBE-LIF surgical models (24.9%). Compared with the normal model, the ROM of the osteoporosis model increased by 4.7%, the maximum stress of the pedicle screw-rod system increased by 14%, and the maximum stress of the endplate increased by 7.5%. Conclusions The results show that the appropriate height of the cage should be selected during the operation to ensure the stability of the segment and avoid the risk of the subsidence caused by the high cage. This is especially important for patients with osteoporosis.

This brief investigates the output-feedback control problem with redundant channels, in which the output measurements may suffer from the Markov analog fading when transmitting through the independent primary channel and redundant channels. The actual channel mode obeys a two-state time-homogeneous Markov chain, and the expectation of fading amplitude in the good mode is much higher than the one in the bad mode. The mode detectors are employed to estimate the status of both the primary and redundant channels, by which the better measurement signals may be selected for the controller. Thus, a token-dependent output-feedback controller is constructed, and then, the stability condition is obtained via the actual mode-dependent Lyapunov function method. Finally, the proposed output-feedback control scheme is applied to an operational amplifier (OPA) circuit under the hidden Markov analog fading channels.

This paper investigates the problem of sliding mode control (SMC) design under the finite-state Markov digital block-fading channels. Before transmitting through the independent digital channels, the output measurements are first quantized through logarithmic quantizers. Then, p independent finite-state hidden Markov models are introduced to model the time-varying effects subject to error-free packet arrival rates. A new one-to-one mapping rule is proposed for reformulating p Markov chains into a new Markov chain. By means of the information from the mode detectors, we construct the detected mode-dependent state observer and the corresponding sliding mode controller. Both the exponential mean-square stability of the closed-loop system and the reachability of the specified sliding surface are analyzed through the fading mode-dependent Lyapunov function method. Furthermore, by adopting the binary genetic algorithm (GA), an optimizing quasi-sliding mode is further achieved. Finally, an illustrative example is provided to verify the effectiveness of the proposed method.

D-amino acids, different from the ubiquitous L-amino acids, are recognized as the “unnatural” amino acids. The applications of D-amino acids have drawn increasing interest from researchers in recent years, and D-amino acids are widely used in various industries, including for food products, pharmaceuticals, and agricultural chemicals. Inspired by the prevalent applications, many synthetic methods for D-amino acids have been developed, which are mainly divided into chemical synthetic methods and biosynthetic methods. Chemical synthesis of D-amino acids has a variety of disadvantages such as multiple reaction steps, low yields, low reaction rates, and difficulties in product extraction. Thus, biosynthetic methods utilizing enzymes are attracting increasing attention because they are more energy-saving and environmentally friendly compared to traditional chemical synthesis. Among all enzymatic methods, multi-enzymatic cascade catalytic methods have significant advantages, such as lower costs, no need for intermediate separation, and higher catalytic efficiency, which is ascribed to the spatial proximity of biocatalysts. In this review, advances in multi-enzyme cascade catalytic systems as well as chemo-enzymatic approaches to synthesize D-amino acids are discussed.

After more than 20 years of technological advancements, the novel field of oil and gas production from source rock strata, which comprise tight and shale oil and gas reservoirs, has become the major contributor to the increase in unconventional oil and gas reserves in China. Accordingly, this field has gradually entered a new stage of revolutionary development. The oil and gas production in China from source rock strata will achieve sustainable development in the future. Different types of source rock strata present distinct challenges and require diverse development paths. Based on the geological conditions of source rock strata in China, this study focuses on identifying the “sweet areas” among hydrocarbon accumulations. It specifically analyzes the key development issues of tight oil, tight gas, shale oil, shale gas, and coal-bed methane, while proposing potential solutions and identifying the possible directions for future development. This study aims to provide a reference for scientists concerned with the development of unconventional oil and gas reserves in China. Cited as : Li, J., Yang, Z., Wu, S., Pan, S. Key issues and development direction of petroleum geology research on source rock strata in China. Advances in Geo-Energy Research, 2021, 5(2): 121-126, doi: 10.46690/ager.2021.02.02

Accumulating evidence have indicated that ferroptosis plays a crucial role in cerebral ischemia-reperfusion (I/R) injury which is the most serious treatment complication of ischemic stroke. Baicalein (5,6,7-trihydroxyflavone) is a main bioactive ingredient isolated from a traditional Chinese medicine named Baikal Skullcap, which is the root of Scutellaria baicalensis Georgi. This study investigated the potential role of baicalein in cerebral I/R injury using oxygen-glucose deprivation and reoxygenation (OGD/R) HT22 cells, transient middle cerebral artery occlusion (tMCAO) mice and RSL3-sitmulated HT22 cells. Baicalein improved the viability of OGD/R cells and significantly ameliorated cerebral I/R injury in tMCAO mice. Baicalein decreased the iron levels, lipid peroxidation production and morphology features of ferroptosis of the brain tissues in tMCAO mice, which indicated that baicalein ameliorated cerebral I/R injury by inhibiting ferroptosis in vivo and in vitro. We further confirmed that baicalein had the activity of inhibiting ferroptosis in RSL3-stimulated HT22 cells. Western blot revealed that baicalein inhibited the ferroptosis by regulating on the expression levels of GPX4, ACSL4 and ACSL3 in OGD/R cells, tMCAO mice and RSL3-stimulated HT22 cells. Our findings demonstrated that baicalein reversed the cerebral I/R injury via anti-ferroptosis, which was regulated by GPX4/ACSL4/ACSL3 axis. The results suggested that baicalein has therapeutic potential as a drug for cerebral I/R injury.

Osteoporoticfractures become increasingly common in postmenopausal women over age 55 years and men after age 65 years, bringing about substantial bone-associated morbidities, and augmented mortality and health-care costs. Advanced researches have led to a more accurate assessment of osteoporosis (OP) and have broadened the range of therapeutic approaches available to prevent osteoporotic fractures. Single-cell RNA sequencing (scRNA-seq) analysis is an evolutionary method that quantifies the majority of transcripts in individual cells at isoform resolution, paving the way for more detailed analyses of gene regulation in biology and medicine. We have extracted 19,102 cells and 4097 dynamical genes with significant expression changes. Several new subtypes of macrophages and differentially over-expressed genes were discovered in the trajectory of osteoclasts formation. The zinc finger protein 36, C3H type-like 1 (ZFP36L1) and defensin alpha 3 (DEFA3) were identified as novel bone metabolism-related genes. RETN-CAP1 was newly found to be involved in the interaction between osteoclasts and immunocytes, indicating that osteo-immunology microenvironment substantially contributed to the pathology of osteoporosis or osteopenia. In this research, we have performed Single-cell RNA sequencing analysis to display the trajectory of osteoclast formation and reveal the possible gene targets and signaling pathways that probably play an important role in osteoporosis.

The blue–green fluorescent carbon quantum dots (BG-CQDs) were successfully synthesized by one-step hydrothermal method using chaff as the only precursor. It is found that the BG-CQDs showed excellent photostability and emitted strong blue–green fluorescence under the excitation wavelength of 330[Formula: see text]nm. And this fluorescence could be effectively quenched by curcumin (CM) based on the static quenching mechanism and inner filter effects with a wider linear range of 2.50–40[Formula: see text][Formula: see text]M and a lower detection limit of 3.53[Formula: see text]nM. Consequently, a sensitive fluorescence probe of BG-CQDs was designed to detect trace CM in blood and urine samples of healthy adults with satisfactory recoveries in the range of 92.07–111.85%. The research extended the applications of biomass waste, especially in the fields of sensor and biomedical monitoring.

Osteoporosis is a disorder of bone metabolism that is extremely common in elderly patients as well as in postmenopausal women. The main manifestation is that the bone resorption capacity is greater than the bone formation capacity, which eventually leads to a decrease in bone mass, increasing the risk of fracture. There is growing evidence that inhibiting osteoclast formation and resorption ability can be effective in treating and preventing the occurrence of osteoporosis. Our study is the first time to explore the role of the mitochondrial calcium uniporter (MCU) and its inhibitor ruthenium red (RR) in bone metabolism, clarifying the specific mechanism by which it inhibits osteoclast formation in vitro and plays a therapeutic role in osteoporosis in vivo. We verified the suppressive effects of RR on the receptor activator of nuclear factor-κB ligand (RANKL-)-induced differentiation and bone resorption function of osteoclasts in vitro. The reactive oxygen species (ROS) production stimulated by RANKL and the expression level of P38 MAPK/NFATc1 were also found to be inhibited by RR. Moreover, the promotion of RR on osteogenesis differentiation was investigated by alkaline phosphatase (ALP) and alizarin red S (ARS) staining and the detection of osteogenesis-specific gene expression levels by quantitative polymerase chain reaction (qPCR) and western blotting. Moreover, in ovariectomy (OVX-)-induced osteoporosis models, RR can downregulate the expression and function of the MCU, relieving bone loss and promoting osteogenesis to present a therapeutic effect on osteoporosis. This new finding will provide an important direction for the study of RR and MCU in the study of bone metabolism therapy targets.

Both poor sleep and diabetic kidney disease are closely associated with inflammation. However, the correlation between poor sleep and diabetic kidney disease has not been well clarified. Thus, the aim of this study was to determine the mediating role that inflammatory markers play in the pathogenic effect of poor sleep on the severity of diabetic kidney disease (DKD). A cross-sectional survey was conducted on 336 patients with type 2 diabetes (T2D). DKD was diagnosed according to the guidelines of the National Kidney Foundation-Kidney Disease Outcome Quality Initiative (NKF-K/DOQI). The Pittsburg Sleep Quality Index (PSQI) score was applied to assess patients for the quality of their sleep. Patients with a PSQI score of more than 5 were assigned to the poor sleep group, and the rest of the patients were assigned to the good sleep group. Circulating levels of six inflammatory biomarkers related to poor sleep and DKD were measured. The prevalence of DKD was higher in patients with poor sleep quality than in those with good sleep quality (42% vs. 25%, P = 0.002). After adjustment, poor sleep quality (PSQI score OR 1.075 [95%CI 1.018–1.135], P = 0.009) remained independently associated with DKD. PSQI score was found to be positively related to fibroblast growth factor (FGF23), interleukin 6 (IL-6), P-selectin, and intercellular adhesion molecule-1 (ICAM-1) (P

China's national cultural characteristics are diverse, and each national culture has its own characteristics and its own development history, which is a highlight of our country. However, in the wave of development of the times, the cultures of many ethnic minorities have gradually merged and degraded. The interaction among heterogeneous cultures is a common phenomenon in human society, and the exchange and collision of different regional cultures is an important factor for culture to thrive. This article is based on a cross-cultural perspective, combined with the special geographical location and diverse geographical environment of Panxi, China, and in-depth discussion, which is the process of cultural change of ethnic minorities in Panxi region of China. The ethnic culture of the Panxi region and the fascinating natural ecology complement each other, creating a unique place where the Tibetan, Qiang, Yi, Naxi, Lisu, and other diverse cultures converge in the ethnic minority areas of China. After the foundation of New China, the social system in Panxi region has undergone earth-shaking changes, and its national culture has undergone drastic changes. With the development of modernization, industrialization, and urbanization, the national culture of Panxi region of China has been constantly changing in the process of cross-cultural interaction, and new characteristics have continuously formed.

Altres ajuts: La Marató de TV3 (20180310) Owing to the recent outbreak of Coronavirus Disease of 2019 (COVID-19), it is urgent to develop effective and safe drugs to treat the present pandemic and prevent other viral infections that might come in the future. Proteins from our own innate immune system can serve as ideal sources of novel drug candidates thanks to their safety and immune regulation versatility. Some host defense RNases equipped with antiviral activity have been reported over time. Here, we try to summarize the currently available information on human RNases that can target viral pathogens, with special focus on enveloped single-stranded RNA (ssRNA) viruses. Overall, host RNases can fight viruses by a combined multifaceted strategy, including the enzymatic target of the viral genome, recognition of virus unique patterns, immune modulation, control of stress granule formation, and induction of autophagy/apoptosis pathways. The review also includes a detailed description of representative enveloped ssRNA viruses and their strategies to interact with the host and evade immune recognition. For comparative purposes, we also provide an exhaustive revision of the currently approved or experimental antiviral drugs. Finally, we sum up the current perspectives of drug development to achieve successful eradication of viral infections.

Aims/hypothesis Glucagon-like peptide 1 receptor agonists (GLP-1 RA) such as exenatide are used as monotherapy and add-on therapy for maintaining glycaemic control in patients with type 2 diabetes mellitus. The current study investigated the safety and efficacy of once-weekly PB-119, a PEGylated exenatide injection, in treatment-naive patients with type 2 diabetes. Methods In this Phase II, randomised, placebo-controlled, double-blind study, we randomly assigned treatment-naive Chinese patients with type 2 diabetes in a 1:1:1:1 ratio to receive subcutaneous placebo or one of three subcutaneous doses of PB-119 (75, 150, and 200 μg) for 12 weeks. The primary endpoint was the change in HbA1c from baseline to week 12, and other endpoints were fasting plasma glucose, 2 h postprandial glucose (PPG), and proportion of patients with HbA1c Results We randomly assigned 251 patients to one of the four treatment groups (n = 62 in placebo and 63 each in PB-119 75 μg, 150 μg and 200 μg groups). At the end of 12 weeks, mean differences in HbA1c in the treatment groups were −7.76 mmol/mol (95% CI −9.23, −4.63, p p p 1c. Similar results were also observed for other efficacy endpoints across different time points. There was no incidence of treatment-emergent serious adverse event, severe hypoglycaemia or death. Conclusions/interpretation All tested PB-119 doses had superior efficacy compared with placebo and were safe and well tolerated over 12 weeks in treatment-naive Chinese patients with type 2 diabetes. Trial registration ClinicalTrials.gov NCT03520972 Funding The study was funded by National Major Scientific and Technological Special Project for Significant New Drugs Development and PegBio. Graphical abstract

Metabolic stability of proteins plays a vital role in various dedicated cellular processes. Traditional methods of measuring the metabolic stability are time-consuming and expensive. Therefore, we developed a more efficient computational approach to understand the protein dynamic action mechanisms in biological process networks. In this study, we collected 341 short-lived proteins and 824 non-short-lived proteins from U2OS; 342 short-lived proteins and 821 non-short-lived proteins from HEK293T; 424 short-lived proteins and 1153 non-short-lived proteins from HCT116; and 384 short-lived proteins and 992 non-short-lived proteins from RPE1. The proteins were encoded by GO and KEGG enrichment scores based on the genes and their neighbors in STRING, resulting in 20,681 GO term features and 297 KEGG pathway features. We also incorporated the protein interaction information from STRING into the features and obtained 19,247 node features. Boruta and mRMR methods were used for feature filtering, and IFS method was used to obtain the best feature subsets and create the models with the highest performance. The present study identified 42 features that did not appear in previous studies and classified them into eight groups according to their functional annotation. By reviewing the literature, we found that the following three functional groups were critical in determining the stability of proteins: synaptic transmission, post-translational modifications, and cell fate determination. These findings may serve as a valuable reference for developing drugs that target protein stability.

The core collection is a small subset that minimizes genetic redundancy while preserving the maximum genetic diversity of the entire population. Research on the core collection is crucial for the efficient management and utilization of germplasm resources. In this paper, the concept of the core collection and the research progress of its construction have briefly been summarized. Subsequently, some perspectives have been proposed in this research field for the near future. Four novel opinions have been presented, (1) the effective integration of multiple data types and accurate phenotyping methods need to be focused on; (2) the sampling strategy and bioinformatics software should be given attention; (3) the core collection of afforestation tree and bamboo species, with a wide natural distribution range and a large planting area, need to be carried out as soon as possible; (4) we should place a high priority on the study of genes discoveries and utilize these with a rapid, precise and high-throughput pattern based on re-sequencing technology. This paper provides a theoretical and technological reference for further study and the application of the plant core collection.