1,135 results on '"Ji Qiu"'
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202. Anchoring Ultrasmall Pd Nanoparticles by Bipyridine Functional Covalent Organic Frameworks for Semihydrogenation of Acetylene
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Ji-qiu ZHANG, Shu-jing Zhang, Yang-qian Lin, Qingqing Guan, and Xi-meng Xu
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History ,Polymers and Plastics ,Business and International Management ,Industrial and Manufacturing Engineering - Published
- 2022
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203. Sludge Decay Kinetics and Metagenomic Analysis Uncover Discrepant Metabolic Mechanisms in Two Different Systems for Sludge in Situ Reduction
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Yanjun Shao, Zhen Zhou, Yi Zuo, Jie Jiang, Yiyue Sun, Junli He, Ji Qiu, Ying An, and Lu-Man Jiang
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- 2022
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204. A Wideband 1-Bit Reconfigurable Reflectarray Antenna at Ku-Band
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Zhou, Shi-Guo, primary, Zhao, Gang, additional, Xu, He, additional, Luo, Chuan-Wei, additional, Sun, Ji-Qiu, additional, Chen, Guan-Tao, additional, and Jiao, Yong-Chang, additional
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- 2022
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205. Anchoring Ultrasmall Pd Nanoparticles by Bipyridine Functional Covalent Organic Frameworks for Semihydrogenation of Acetylene
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ZHANG, Ji-qiu, primary, Zhang, Shu-jing, additional, Lin, Yang-qian, additional, Guan, Qingqing, additional, and Xu, Xi-meng, additional
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- 2022
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206. A filtering antenna with wide out‐of‐band suppression based on open‐stubs resonator
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Ji‐Qiu Sun, Li Zhang, Chuan‐Wei Luo, Shi‐Guo Zhou, and Yong‐Chang Jiao
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Electrical and Electronic Engineering ,Computer Graphics and Computer-Aided Design ,Computer Science Applications - Published
- 2021
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207. Voxel-Based Morphometric MRI Postprocessing-Assisted Laser Interstitial Thermal Therapy for Focal Cortical Dysplasia-Suspected Lesions: Technique and Outcomes
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Wen-han Hu, Jia-jie Mo, Bo-wen Yang, Huan-guang Liu, Chao Zhang, Xiu Wang, Jia-ji Qiu, Bao-tian Zhao, Xiao-qiu Shao, Jian-guo Zhang, and Kai Zhang
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Malformations of Cortical Development ,Epilepsy ,Lasers ,Humans ,Surgery ,Neurology (clinical) ,Laser Therapy ,Magnetic Resonance Imaging - Abstract
MRI-guided laser interstitial thermal therapy (MRgLITT) is a novel treatment modality for focal cortical dysplasia (FCD). However, identifying the location and extent of subtle FCD by visual analysis during MRgLITT remains challenging.To introduce voxel-based morphometric MRI postprocessing into the procedure of MRgLITT for FCD-suspected lesions and assess the complementary value of the MRI postprocessing technique for the trajectory design and thermal parameter setting of MRgLITT.Junction and normalized fluid-attenuated inversion recovery signal intensity images were used to detect the gray-white matter junction blurring and cortical fluid-attenuated inversion recovery hyperintensity, respectively. According to the 2 postprocessing images, the region of interest (ROI) for ablation was drawn. The main principle of presurgical planning is that the trajectory of the laser fiber was designed as far as possible along the long axis of the ROI while the extent of planned ablation covered the entire ROI. The subsequent intraoperative procedure was performed under the guidance of the presurgical plan.Nine patients with epilepsy with FCD-suspected lesions underwent MRgLITT with the assistance of MRI postprocessing images. Among them, 4 patients were junction positive, 2 patients were normalized fluid-attenuated inversion recovery signal intensity positive, and the remaining 3 patients were positive for both. Postsurgical MRI demonstrated that the ROIs were ablated entirely in 7 patients. Engel Ia, Ib, and IV scores were obtained at 1-year follow-up for 6, 1, and 2 patients, respectively.MRI postprocessing provides complementary information for designing the laser fiber trajectory and subsequent ablation for FCDs.
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- 2021
208. Mooring Stone-Like Arg
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Can, Yue, Wangzhen, Xiang, Xiaowen, Huang, Yuan, Sun, Jin, Xiao, Kefang, Liu, Zeyu, Sun, Peiwen, Qiao, Hongmei, Li, Jingxuan, Gan, Limin, Ba, Yan, Chai, Jianxun, Qi, Peipei, Liu, Peng, Qi, Yingze, Zhao, Yongfeng, Li, Hua-Ji, Qiu, George F, Gao, Guolan, Gao, and William J, Liu
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Antigen Presentation ,Binding Sites ,Protein Conformation ,Swine ,Structure and Assembly ,Histocompatibility Antigens Class I ,Epitopes, T-Lymphocyte ,Arginine ,Lymphocyte Activation ,African Swine Fever Virus ,Foot-and-Mouth Disease Virus ,Animals ,Capsid Proteins ,Peptides ,Protein Binding ,T-Lymphocytes, Cytotoxic - Abstract
African swine fever virus (ASFV) is the causative agent of African swine fever (ASF), which is a devastating pig disease threatening the global pork industry. However, currently, no commercial vaccines are available. During the pig immune response, major histocompatibility complex class I (MHC-I) molecules select viral peptide epitopes and present them to host cytotoxic T lymphocytes, thereby playing critical roles in eliminating viral infections. Here, we screened peptides derived from ASFV and determined the molecular basis of ASFV-derived peptides presented by the swine leukocyte antigen 1*0101 (SLA-1*0101). We found that peptide binding in SLA-1*0101 differs from the traditional mammalian binding patterns. Unlike the typical B and F pockets used by the common MHC-I molecule, SLA-1*0101 uses the D and F pockets as major peptide anchor pockets. Furthermore, the conformationally stable Arg(114) residue located in the peptide-binding groove (PBG) was highly selective for the peptides. Arg(114) draws negatively charged residues at positions P5 to P7 of the peptides, which led to multiple bulged conformations of different peptides binding to SLA-1*0101 and creating diversity for T cell receptor (TCR) docking. Thus, the solid Arg(114) residue acts as a “mooring stone” and pulls the peptides into the PBG of SLA-1*0101. Notably, the T cell recognition and activation of p72-derived peptides were verified by SLA-1*0101 tetramer-based flow cytometry in peripheral blood mononuclear cells (PBMCs) of the donor pigs. These results refresh our understanding of MHC-I molecular anchor peptides and provide new insights into vaccine development for the prevention and control of ASF. IMPORTANCE The spread of African swine fever virus (ASFV) has caused enormous losses to the pork industry worldwide. Here, a series of ASFV-derived peptides were identified, which could bind to swine leukocyte antigen 1*0101 (SLA-1*0101), a prevalent SLA allele among Yorkshire pigs. The crystal structure of four ASFV-derived peptides and one foot-and-mouth disease virus (FMDV)-derived peptide complexed with SLA-1*0101 revealed an unusual peptide anchoring mode of SLA-1*0101 with D and F pockets as anchoring pockets. Negatively charged residues are preferred within the middle portion of SLA-1*0101-binding peptides. Notably, we determined an unexpected role of Arg(114) of SLA-1*0101 as a “mooring stone” which pulls the peptide anchoring into the PBG in diverse “M”- or “n”-shaped conformation. Furthermore, T cells from donor pigs could activate through the recognition of ASFV-derived peptides. Our study sheds light on the uncommon presentation of ASFV peptides by swine MHC-I and benefits the development of ASF vaccines.
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- 2021
209. Current efforts towards safe and effective live attenuated vaccines against African swine fever: challenges and prospects
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Tao Wang, Rui Luo, Yuan Sun, and Hua-Ji Qiu
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Differentiation of infected from vaccinated animals ,Efficacy ,Swine ,Sus scrofa ,Public Health, Environmental and Occupational Health ,Viral Vaccines ,General Medicine ,Infectious and parasitic diseases ,RC109-216 ,Vaccines, Attenuated ,African Swine Fever Virus ,Infectious Diseases ,Live attenuated vaccine ,Biosecurity ,Commentary ,Animals ,Public aspects of medicine ,RA1-1270 ,African swine fever ,Safety - Abstract
Background African swine fever (ASF) is a fatal hemorrhagic disease in domestic pigs and wild boar caused by African swine fever virus (ASFV). Since ASF has been introduced into Europe and Asia, the major pig-raising areas, posing a huge threat to the pork industry worldwide. Currently, prevention and control of ASF are basically dependent on strict biosecurity measures and stamping-out policy once ASF occurs. Main text The major risks of ASF spread are insufficient biosecurity measures and human behaviors. Therefore, a safe and effective vaccine seems to be a reasonable demand for the prevention and control of ASF. Due to the efficacy advantage over other types of vaccines, live attenuated vaccines (LAVs), especially virulence-associated genes deleted vaccines, are likely to be put into emergency and conditional use in restricted areas if ASF is out of control in a country with a huge pig population and pork consumption, like China. However, the safety, efficacy, and genetic stability of current candidate ASF LAVs require comprehensive clinical evaluations prior to country-wide field application. Several critical issues need to be addressed to commercialize an ideal ASF LAV, including a stable cell line for manufacturing vaccines, differentiation of infected from vaccinated animals (DIVA), and cross-protection from different genotypes. Conclusion A safe and effective DIVA vaccine and an accompanying diagnostic assay will facilitate the prevention, control, and eradication of ASF, which is quite challenging in the near future. Graphical Abstract
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- 2021
210. The Unique Glycosylation at Position 986 on the E2 Glycoprotein of Classical Swine Fever Virus Is Responsible for Viral Attenuation and Protection against Lethal Challenge
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Yuzi Luo, Hua-Ji Qiu, Mengqi Yuan, Yuteng Ma, Yongfeng Li, Weike Li, Yuan Sun, Su Li, Libao Xie, and Yuying Han
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Glycosylation ,Swine ,Immunology ,Virulence ,Vaccines, Attenuated ,Virus Replication ,Microbiology ,Virus ,Classical Swine Fever ,chemistry.chemical_compound ,Viral Envelope Proteins ,Virology ,Animals ,chemistry.chemical_classification ,Infectivity ,Attenuated vaccine ,biology ,Viral Vaccines ,biology.organism_classification ,Antibodies, Neutralizing ,chemistry ,Viral replication ,Classical swine fever ,Classical Swine Fever Virus ,Insect Science ,Mutation ,Immunization ,Rabbits ,Protein Multimerization ,Glycoprotein - Abstract
Classical swine fever (CSF) is an economically important disease of pigs caused by the classical swine fever virus (CSFV). The live attenuated vaccine C-strain (also called HCLV strain) against CSF was produced by multiple passages of a highly virulent strain in rabbits. However, the molecular determinants for its attenuation and protection remain unclear. In this study, we identified a unique glycosylation at position 986 (986NYT988) on the E2 glycoprotein Domain IV of C-strain but not (986NYA988) the highly virulent CSFV Shimen strain. We evaluated the infectivity, virulence, and protective efficacy of the C-strain-based mutant rHCLV-T988A lacking the glycosylation and Shimen strain mutant rShimen-A988T acquiring an additional glycosylation at position 986. rShimen-A988T showed a significantly decreased viral replication ability in SK6 cells, while rHCLV-T988A exhibited a growth kinetics indistinguishable from that of C-strain. Removal of the C-strain glycosylation site does not affect viral replication in rabbits and the attenuated phenotype in pigs. However, rShimen-A988T was attenuated and protected the pigs from a lethal challenge at 14 days post-inoculation. In contrast, the rHCLV-T988A-inoculated pigs showed transient fever, a few clinical signs, and pathological changes in the spleens upon challenge with the Shimen strain. Mechanistic investigations revealed that the unique glycosylation at position 986 influences viral spreading, alters the formation of E2 homodimers, and leads to increased production of neutralizing antibodies. Collectively, our data for the first time demonstrate that the unique glycosylation at position 986 on the E2 glycoprotein is responsible for viral attenuation and protection. IMPORTANCE Viral glycoproteins involve in infectivity, virulence, and host immune responses. Deglycosylation on the Erns, E1, or E2 glycoprotein of highly virulent classical swine fever virus (CSFV) attenuated viral virulence in pigs, indicating that the glycosylation contributes to the pathogenicity of highly virulent strain. However, the effects of the glycosylation on the C-strain E2 glycoprotein on viral infectivity in cells, viral attenuation, and protection in pigs have not been elucidated. This study demonstrates the unique glycosylation at position 986 on the C-strain E2 glycoprotein. C-strain mutant removing the glycosylation at the site provides only partial protection against CSFV challenge. Remarkably, the addition of the glycan to E2 of the highly virulent Shimen strain attenuates the viral virulence and confers complete protection against the lethal challenge in pigs. Our findings provide a new insight into the contribution of the glycosylation to the virus attenuation and protection.
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- 2021
211. Rationally designed Mn2O3@ZnMn2O4/C core–shell hollow microspheres for aqueous zinc-ion batteries.
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Gao, Zhan, Lu, Guo-ge, Cao, Liu-cheng, Zhu, Zong-xiu, Li, Ying-xin, Wei, Fu-xiang, Ji, Zhe, Sui, Yan-wei, Qi, Ji-qiu, Meng, Qing-kun, and Ren, Yao-jian
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MICROSPHERES ,CATHODES ,ZINC ions ,HIGH voltages ,STORAGE batteries - Abstract
Manganese-based oxides are common cathode materials for aqueous zinc ion batteries (AZIBs) because of their great capacity and high working voltage. However, the sharp decline of capacity caused by the dissolution of manganese-based cathode materials and the low-rate performance restrict their development. To address these problems, unique core–shell structured Mn
2 O3 @ZnMn2 O4 /C hollow microspheres are reported as an ideal cathode material for AZIBs. Benefiting from the hollow structure, the zeolitic imidazolate framework (ZIF)-derived carbon and ZnMn2 O4 . Its application in AZIBs as the cathode demonstrates its satisfactory rate performance, high cycle stability, and excellent reversibility. Its high reversible capacity is remarkable, which reaches its maximum of 289.9 mA h g−1 at 200 mA g−1 and maintains a capacity of 203.5 mA h g−1 after cycling for 700 times at 1000 mA g−1 . These excellent performances indicate that this material is a potential cathode material of AZIBs. [ABSTRACT FROM AUTHOR]- Published
- 2023
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212. Different Types of Vaccines against Pestiviral Infections: 'Barriers' for 'Pestis'
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Mengqi Yuan, Xiaoke Yang, Xin Zhang, Xiaotian Zhao, Muhammad Abid, Hua-Ji Qiu, and Yongfeng Li
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Infectious Diseases ,Virology - Abstract
The genus Pestivirus of the family Flaviviridae mainly comprises classical swine fever virus (CSFV), bovine viral diarrhea virus 1 (BVDV-1), BVDV-2, border disease virus (BDV), and multiple new pestivirus species such as atypical porcine pestivirus (APPV), giraffe pestivirus, and antelope pestivirus. Pestiviruses cause infectious diseases, resulting in tremendous economic losses to animal husbandry. Different types of pestivirus vaccines have been developed to control and prevent these important animal diseases. In recent years, pestiviruses have shown great potential as viral vectors for developing multivalent vaccines. This review analyzes the advantages and disadvantages of various pestivirus vaccines, including live attenuated pestivirus strains, genetically engineered marker pestiviruses, and pestivirus-based multivalent vaccines. This review provides new insights into the development of novel vaccines against emerging pestiviruses, such as APPV and ovine pestivirus.
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- 2022
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213. Sludge decay kinetics and metagenomic analysis uncover discrepant metabolic mechanisms in two different sludge in situ reduction systems
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Yanjun, Shao, Zhen, Zhou, Yi, Zuo, Jie, Jiang, Lihua, Wang, Yiyue, Sun, Junli, He, Ji, Qiu, Ying, An, and Lu-Man, Jiang
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Environmental Engineering ,Sewage ,L-Lactate Dehydrogenase ,Hydrolases ,Waste Disposal, Fluid ,Pollution ,Kinetics ,Bioreactors ,Adenosine Triphosphate ,Environmental Chemistry ,Environmental Pollutants ,Anaerobiosis ,Citrates ,Waste Management and Disposal - Abstract
A comparative study was conducted between an anaerobic side-stream reactor (ASSR) process and a sludge process reduction (SPR) activated sludge (SPRAS) process for uncovering crucial metabolic mechanisms governing sludge reduction. Both of two processes were efficient in removing pollutants, while the SPRAS (62.3 %) obtained much higher sludge reduction than the ASSR (27.9 %). The highest rate coefficients of sludge decay, heterotroph lysis and particles hydrolysis were 0.106, 0.219 and 0.054 d
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- 2022
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214. First Identification and Phylogenetic Analysis of Porcine Circovirus Type 4 in Fur Animals in Hebei, China
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Yanjin Wang, Shijie Yan, Yuting Ji, Yujie Yang, Ping Rui, Zengjun Ma, Hua-Ji Qiu, and Tao Song
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fur animals ,porcine circovirus type 4 ,phylogenetic analysis ,General Veterinary ,Animal Science and Zoology - Abstract
A novel circovirus called porcine circovirus type 4 (PCV4) was recently detected in pigs suffering from severe clinical diseases in Hunan province, China. There are few reports on the origin and evolution of PCV4, although some researchers have conducted epidemiological investigations of PCV4 and found that PCV4 is widespread in pigs. Based on the previous study, we detected PCV2 in farmed foxes and raccoon dogs with reproductive failure. To explore whether the PCV4 genome also exists in fur animals, we detected 137 cases admitted from fur animal farms in Hebei China between 2015 and 2020, which were characterized by inappetence, lethargy, depression, abortion, and sterility. The overall infection rate of PCV4 was 23.36% (32/137), including 20.37% (22/108) for raccoon dogs, 18.75% (3/16) for foxes, and 53.85% (7/13) for minks. Finally, five raccoon dog-origin PCV4 strains and one fox-origin PCV4 strain were sequenced in our study, whose nucleotide identities with other representative PCV4 strains varied from 96.5% to 100%. Phylogenetic analysis based on the complete genomes of PCV4 strains indicated a close relationship with those of PCV4 strains identified from pigs. To our knowledge, this is the first study to detect PCV4 in fur animals. Interestingly, we also identified PCV4 in a mixed farm (feeding pigs and raccoon dogs at the same time). In summary, our findings extend the understanding of the molecular epidemiology of PCV4 and provide new evidence for its cross-species transmission.
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- 2022
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215. DLP Learning from Uncertain Data
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Zhu, Man, Gao, Zhiqiang, Qi, Guilin, and Ji, Qiu
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- 2010
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216. Biocompatibility of a chitosan-based injectable thermosensitive hydrogel and its effects on dog periodontal tissue regeneration
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Ji, Qiu Xia, Deng, Jing, Xing, Xiao Ming, Yuan, Chang Qing, Yu, Xin Bo, Xu, Quan Chen, and Yue, Jin
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- 2010
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217. Determining an Improved Traffic Conflict Indicator for Highway Safety Estimation Based on Vehicle Trajectory Data
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Hongguang Chang, Ji Qiu, Jiang Ruoxi, Naikan Ding, Bing Liu, Jingan Wu, and Shunying Zhu
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traffic conflict indicator ,Correlation coefficient ,Computer science ,Traffic conflict ,UAV ,Geography, Planning and Development ,highway ,traffic safety estimation ,TJ807-830 ,Crash ,Management, Monitoring, Policy and Law ,computer.software_genre ,TD194-195 ,Renewable energy sources ,Correlation ,vehicle trajectory data ,traffic conflict technique ,GE1-350 ,Correlation test ,Estimation ,Environmental effects of industries and plants ,Renewable Energy, Sustainability and the Environment ,Environmental sciences ,Correlation analysis ,Trajectory ,Data mining ,computer - Abstract
Currently, several traffic conflict indicators are used as surrogate safety measures. Each indicator has its own advantages, limitations, and suitability. There are only a few studies focusing on fixed object conflicts of highway safety estimation using traffic conflict technique. This study investigated which conflict indicator was more suitable for traffic safety estimation based on conflict-accident Pearson correlation analysis. First, a high-altitude unmanned aerial vehicle was used to collect multiple continuous high-precision videos of the Jinan-Qingdao highway. The vehicle trajectory data outputted from recognition of the videos were used to acquire conflict data following the procedure for each conflict indicator. Then, an improved indicator Ti was proposed based on the advantages and limitations of the conventional indicators. This indicator contained definitions and calculation for three types of traffic conflicts (rear-end, lane change and with fixed object). Then the conflict-accident correlation analysis of TTC (Time to Collision)/PET (Post Encroachment Time)/DRAC (Deceleration Rate to Avoid Crash)/Ti indicators were carried out. The results show that the average value of the correlation coefficient for each indicator with different thresholds are 0.670 for TTC, 0.669 for PET, and 0.710 for DRAC, and 0.771 for Ti, which Ti indicator is obviously higher than the other three conventional indicators. The findings of this study suggest TTC often fails to identify lane change conflicts, PET indicator easily misjudges some rear-end conflict when the speed of the following vehicle is slower than the leading vehicle, and PET is less informative than other indicators. At the same time, these conventional indicators do not consider the vehicle-fixed objects conflicts. The improved Ti can overcome these shortcomings, thus, Ti has the highest correlation. More data are needed to verify and support the study.
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- 2021
218. [Complex structures and diverse functions of nuclear bodies: a review]
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Xinyuan, Liu, Lian-Feng, Li, and Hua-Ji, Qiu
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Cell Nucleus ,Intranuclear Inclusion Bodies ,Animals ,Nuclear Proteins ,Epigenesis, Genetic - Abstract
Nuclear bodies are membrane-free nuclear substructures that are localized in the mammalian nuclear matrix region. They are multiprotein complexes that recruit other proteins to participate in various cellular activities, such as transcription, RNA splicing, epigenetic regulation, tumorigenesis and antiviral defense. It is of great significance to clarify the functions and regulatory mechanisms of nuclear bodies to probe related diseases and virus-host interactions. This review takes several nuclear bodies associated proteins as examples, summarizes the formation process, structure and functions of nuclear bodies, and focuses on their important roles in antiviral infection. It is expected to provide new insight into host antiviral mechanisms.
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- 2021
219. A filtering antenna with wide out‐of‐band suppression based on open‐stubs resonator
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Sun, Ji‐Qiu, primary, Zhang, Li, additional, Luo, Chuan‐Wei, additional, Zhou, Shi‐Guo, additional, and Jiao, Yong‐Chang, additional
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- 2021
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220. Distal mutation V486M disrupts the catalytic activity of DPP4 by affecting the flap of the propeller domain
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Li, Teng-teng, primary, Peng, Cheng, additional, Wang, Ji-qiu, additional, Xu, Zhi-jian, additional, Su, Ming-bo, additional, Li, Jia, additional, Zhu, Wei-liang, additional, and Li, Jing-ya, additional
- Published
- 2021
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221. Model fitting of the seasonal population dynamics of the soybean aphid, Aphis glycinesMatsumura, in the field
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XU, Lei, ZHAO, Tong-hua, Xing, Xing, XU, Guo-qing, XU, Biao, and ZHAO, Ji-qiu
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The soybean aphid, Aphis glycinesMatsumura (Hemiptera: Aphididae), is one of the greatest threats to soybean production, and both trend analysis and periodic analysis of its population dynamics are important for integrated pest management (IPM). Based on systematically investigating soybean aphid populations in the field from 2018 to 2020, this study adopted the inverse logistic model for the first time, and combined it with the classical logistic model to describe the changes in seasonal population abundance from colonization to extinction in the field. Then, the increasing and decreasing phases of the population fluctuation were divided by calculating the inflection points of the models, which exhibited distinct seasonal trends of the soybean aphid populations in each year. In addition, multifactor logistic models were then established for the first time, in which the abundance of soybean aphids in the field changed with time and relevant environmental conditions. This model enabled the prediction of instantaneous aphid abundance at a given time based on relevant meteorological data. Taken as a whole, the successful approaches implemented in this study could be used to build a theoretical framework for practical IPM strategies for controlling soybean aphids.
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- 2023
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222. Establishment of intelligent prompt system of precise flushing in pharmacy intravenous admixture service
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Ting Liu, Zhangwei Yang, Ji Qiu, Wenjing Zhu, Fu Dai, and Aihong Pan
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Pharmacology ,Service (business) ,business.industry ,Drug Discovery ,medicine ,Pharmaceutical Science ,Flushing ,Pharmacy ,Medical emergency ,medicine.symptom ,business ,medicine.disease - Published
- 2019
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223. Sjogren‐Larsson syndrome associated hypermelanosis
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Ping Li, Yang-Chun Xu, Ji-qiu Hou, and Wen-Jing Zhu
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Keratinocytes ,Enzyme complex ,Fatty alcohol ,Dermatology ,Bioinformatics ,medicine.disease_cause ,Mice ,030207 dermatology & venereal diseases ,03 medical and health sciences ,chemistry.chemical_compound ,Fatty aldehyde ,0302 clinical medicine ,Hyperpigmentation ,Animals ,Humans ,Medicine ,Cell Proliferation ,Skin ,Melanins ,Mice, Knockout ,Aldehydes ,Sphingolipids ,Sjögren–Larsson syndrome ,business.industry ,Fatty Acids ,medicine.disease ,Aldehyde Oxidoreductases ,Sphingolipid ,Disease Models, Animal ,Oxidative Stress ,Sjogren-Larsson Syndrome ,medicine.anatomical_structure ,chemistry ,030220 oncology & carcinogenesis ,Skin hyperpigmentation ,Mutation ,Melanocytes ,Reactive Oxygen Species ,business ,Keratinocyte ,Oxidative stress - Abstract
Background/objectives Sjogren - Larsson syndrome (SLS) is a rare autosomal recessive disease of the mutation ALDH3A2 that identifies a part of fatty acids for fatty aldehyde dehydrogenase: NAD-oxidoreductase enzyme complex. This study aimed to access variant ALDH3A2 gene coded for FALDH and products regulating pathogenic melanogenesis owing to increased oxidative stress and reactive oxygen species resulting in DNA harm in SLS. By turning them into fatty acids, FALDH avoids the accumulation of toxic fatty aldehydes. The mutation results in the accumulation of aldehyde-modified lipids or fatty alcohols that may interfere with skin and brain function. Methods In Nov 2018, we performed a literature search in PubMed for clinical studies, clinical trials, case reports, controlled trials, randomized controlled trials, and systemic reviews. The search terms we used were "SJOGREN-LARSSON SYNDROME" AND "HYPERMELANNOSIS" OR "FALDH" (from 1985). The search resulted in 1,289 articles, out of these 95 articles met our inclusion exclusion criteria. Our inclusion criteria included relevant original articles relevant, critical systemic reviews, and crucial referenced articles, ex-clusion criteria included duplicates and articles not published in English language. Results Toxicity of long-chain aldehydes to FALDH-deficient cells owing to accumulation under the profound epidermis layer improves oxidative stress in the cell resulting in keratinocyte hyperproliferation. Conclusion While it continues to be determined whether accumulated fatty alcohol and fatty aldehydes obtained from ether glycerolipids and sphingolipids improve the susceptibility of melanocytes and their element accountable for skin hyperpigmentation to biological colour.
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- 2019
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224. Effects of pre-compression on the hardness of CoCrFeNiMn high entropy alloy based an asymmetrical yield criterion
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Buyun Su, Dan Zhao, Ji Qiu, Gesheng Xiao, Tao Jin, Erqiang Liu, Xuefeng Shu, and Z.H. Wang
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Materials science ,Yield (engineering) ,Mechanical Engineering ,Alloy ,Metals and Alloys ,02 engineering and technology ,Plasticity ,Nanoindentation ,engineering.material ,010402 general chemistry ,021001 nanoscience & nanotechnology ,Compression (physics) ,01 natural sciences ,Grain size ,0104 chemical sciences ,Mechanics of Materials ,Geometrically necessary dislocations ,Materials Chemistry ,engineering ,Pre compression ,Composite material ,0210 nano-technology - Abstract
In this investigation, an asymmetrical yield criterion is proposed, and applies it to a hardness model which considers the interaction between statistically stored dislocations (SSDs) and geometrically necessary dislocations (GNDs). Based on this modified model, the relationship between pre-compression strain and hardness is analyzed by nanoindentation tests. In order to obtain the yield behavior of the CoCrFeNiMn high entropy alloy (HEA), the combined loading and uniaxial loading tests are conducted. Then different compression plastic strain is applied to specimens under various levels of temperature to investigate the interaction between SSDs and GNDs of CoCrFeNiMn HEA. Finally, the result shows that the effect of pre-compression depends on the competition between the pre-compression induced dislocations and nanoindentation nucleated dislocations because pre-compression causes the change of grain size.
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- 2019
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225. Discovery of an autoantibody signature for the early diagnosis of knee osteoarthritis: data from the Osteoarthritis Initiative
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F. Picchi, Vanesa Balboa-Barreiro, Natividad Oreiro, Manuel Fuentes, M. Camacho-Encina, Joshua LaBaer, Ignacio Rego-Pérez, Jennifer VanDuin, Cristina Ruiz-Romero, Francisco J. Blanco, Ji Qiu, Centro de Investigación Biomédica en Red Cáncer (España), Instituto de Salud Carlos III, Xunta de Galicia, and European Commission
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Male ,musculoskeletal diseases ,0301 basic medicine ,medicine.medical_specialty ,Knee Joint ,autoantibodies ,diagnosis ,Immunology ,Enzyme-Linked Immunosorbent Assay ,Osteoarthritis ,Disease ,Logistic regression ,General Biochemistry, Genetics and Molecular Biology ,03 medical and health sciences ,0302 clinical medicine ,Rheumatology ,Predictive Value of Tests ,Internal medicine ,medicine ,Humans ,Immunology and Allergy ,030203 arthritis & rheumatology ,Receiver operating characteristic ,business.industry ,Incidence ,Incidence (epidemiology) ,Autoantibody ,biomarkers ,Middle Aged ,Osteoarthritis, Knee ,medicine.disease ,respiratory tract diseases ,Radiography ,Early Diagnosis ,030104 developmental biology ,ROC Curve ,Spain ,Case-Control Studies ,Disease Progression ,Prognostic model ,Female ,prognosis ,Biostatistics ,business ,Follow-Up Studies - Abstract
[Objective]: To find autoantibodies (AAbs) in serum that could be useful to predict incidence of radiographic knee osteoarthritis (KOA)., [Design]: A Nucleic-acid Programmable Protein Arrays (NAPPA) platform was used to screen AAbs against 2125 human proteins in sera at baseline from participants free of radiographic KOA belonging to the incidence and non-exposed subcohorts of the Osteoarthritis Initiative (OAI) who developed or not, radiographic KOA during a follow-up period of 96 months. NAPPA-ELISA were performed to analyse reactivity against methionine adenosyltransferase two beta (MAT2β) and verify the results in 327 participants from the same subcohorts. The association of MAT2β-AAb levels with KOA incidence was assessed by combining several robust biostatistics analysis (logistic regression, Receiver Operating Characteristic and Kaplan-Meier curves). The proposed prognostic model was replicated in samples from the progression subcohort of the OAI., [Results]: In the screening phase, six AAbs were found significantly different at baseline in samples from incident compared with non-incident participants. In the verification phase, high levels of MAT2β-AAb were significantly associated with the future incidence of KOA and with an earlier development of the disease. The incorporation of this AAb in a clinical model for the prognosis of incident radiographic KOA significantly improved the identification/classification of patients who will develop the disorder. The usefulness of the model to predict radiographic KOA was confirmed on a different OAI subcohort., [Conclusions]: The measurement of AAbs against MAT2β in serum might be highly useful to improve the prediction of OA development, and also to estimate the time to incidence., This work has been funded by grants from Fondo Investigación Sanitaria-Spain (PI14/01707, PI16/02124, PI17/00404, DTS17/00200, CIBER-BBN CB06/01/0040, CIBER-ONC CB16/12/00400, RETIC-RIERRD12/0009/0018), a part of the National Plan for Scientific Program Development and Technological Innovation 2013-2016, funded by the ISCIII-General Subdirection of Assessment and Promotion of Research - European Regional Development Fund (FEDER) >A way of making Europe> . MC-E is supported by the Xunta de Galicia and the European Union (European Social Fund – ESF) through a predoctoral fellowship (IN606A-2016/012). IR-P and CR-R were supported by the Miguel Servet II programme from Fondo Investigación Sanitaria- Spain (CPII17/0026 and CPII15/00013, respectively).
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- 2019
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226. A Criterion Describing the Dynamic Yield Behavior of PMMA
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Erqiang Liu, Xin Li, Qian Duan, Ji Qiu, Tao Jin, Xuefeng Shu, and Buyun Su
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Yield (engineering) ,Materials science ,Polymers and Plastics ,Tension (physics) ,General Chemical Engineering ,media_common.quotation_subject ,Organic Chemistry ,02 engineering and technology ,010402 general chemistry ,021001 nanoscience & nanotechnology ,Compression (physics) ,01 natural sciences ,Asymmetry ,0104 chemical sciences ,Dynamic loading ,Materials Chemistry ,Sensitivity (control systems) ,Composite material ,Hydrostatic stress ,0210 nano-technology ,media_common - Abstract
Dynamic uniaxial and multiaxial tests were conducted to characterize the mechanical behavior of poly(methyl methacrylate) (PMMA). In this case, the multiaxial yield behavior of materials can be captured by the shear-compression samples (SCS). The experimental results were compared with different yield criteria, and which does not have a good agreement between theoretical prediction and experimental yield loci of PMMA undergoing combined shear-compression. Therefore, a phenomenological yield criterion is developed, and it notably involves hydrostatic stress sensitivity, lode angle, and one parameter. The parameter can be expressed by the asymmetry of yield strength between the tension and compression. The proposed criterion was verified by the utilization of differently combined shear-compression tests. The investigative results reveal that the proposed criterion can be successfully applied to describe the complex yield behavior of PMMA under dynamic loading conditions. The present study shows an efficient method to develop yield criterion of the reviewed materials.
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- 2019
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227. Microstructure and corrosion resistance of the PEO coating on extruded Al6Cu alloy
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Chen Zhixiong, Fuhui Wang, Tao Zhang, Ji Qiu, Wei Zhang, Liye Zhu, and Jinhui Chen
- Subjects
Materials science ,020209 energy ,Alloy ,02 engineering and technology ,Surfaces and Interfaces ,General Chemistry ,Substrate (electronics) ,engineering.material ,021001 nanoscience & nanotechnology ,Condensed Matter Physics ,Microstructure ,Surfaces, Coatings and Films ,Corrosion ,Coating ,Phase (matter) ,Volume fraction ,0202 electrical engineering, electronic engineering, information engineering ,Materials Chemistry ,engineering ,Composite material ,0210 nano-technology ,Porosity - Abstract
The morphology and anti-corrosion performance of the PEO coating on extruded Al6Cu alloy was investigated by means of microscopy observation and electrochemical measurements. The results showed that the PEO coating formed on extruded Al6Cu alloy had superior anti-corrosion performance compared to as-cast Al6Cu alloy. It could be ascribed to the microstructure change in substrate, wherein the reduced continuity, size and volume fraction of θ phase decreased the oxygen evolution during the PEO process. The oxygen release promoted the formation of pores and cracks in the PEO coating. Consequently, the lower porosity of the PEO coating on the extruded Al6Cu alloy provided superior protective performance.
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- 2019
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228. Effects of titanium addition on microstructure and mechanical properties of CrFeNiTix (x = 0.2–0.6) compositionally complex alloys
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Xiao Chen, Ye Zeng He, Teng Kong, Shuo Gao, Qing Kun Meng, Ji Qiu Qi, Yao Jian Ren, Yan Wei Sui, Man Zhang, Fu Xiang Wei, and Zhi Sun
- Subjects
Materials science ,Mechanical Engineering ,Alloy ,R-Phase ,engineering.material ,Condensed Matter Physics ,Microstructure ,Dendrite (crystal) ,Solid solution strengthening ,Precipitation hardening ,Mechanics of Materials ,Phase (matter) ,engineering ,General Materials Science ,Composite material ,Solid solution - Abstract
CrFeNiTix (x = 0.2, 0.3, 0.4, 0.5, and 0.6 molar ratio) compositionally complex alloys were fabricated by vacuum arc melting to investigate the microstructure, hardness, and compressive properties. The results revealed that CrFeNiTix alloys consisted of the principal face-centered cubic (FCC) phase and body-centered cubic (BCC) solid solution, with an amount of (Ni, Ti)-rich hexagonal close-packed phase. CrFeNiTix alloys exhibited the typical dendrite. Ti0.2 and Ti0.3 alloys were composed of FCC and BCC solid solutions in the dendrite, as well as e (Ni3Ti) and R (Ni2.67Ti1.33) phases in the inter-dendrite, simultaneously. For Ti0.4, Ti0.5, and Ti0.6 alloys, (Fe, Cr)-rich solid solution separated out and e phase transformed into R phase gradually. Meanwhile, TEM analysis indicated that Ti0.4 alloy matrix consisted of the principal FCC phase containing (Ni, Ti)-rich intragranular nanoprecipitates. The hardness values of CrFeNiTix alloys were increased with the addition of Ti content and the high compressive strength of CrFeNiTix alloys was maintained, which was attributed to the solid solution strengthening and precipitation hardening.
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- 2019
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229. The browning of white adipose tissue and body weight loss in primary hyperparathyroidismResearch in context
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Yang He, Rui-xin Liu, Min-ting Zhu, Wen-bin Shen, Jing Xie, Zhi-yin Zhang, Na Chen, Chang Shan, Xing-zhi Guo, Yi-de Lu, Bei Tao, Li-hao Sun, Hong-yan Zhao, Rui Guo, Biao Li, Si-min Liu, Guang Ning, Ji-qiu Wang, and Jian-min Liu
- Subjects
lcsh:R5-920 ,endocrine system diseases ,lcsh:R ,lcsh:Medicine ,lcsh:Medicine (General) ,hormones, hormone substitutes, and hormone antagonists - Abstract
Background: Parathyroid hormone related protein (PTHrP) triggers white adipose tissue (WAT) browning and cachexia in lung cancer mouse models. It remains unknown whether excessive PTH secretion affects WAT browning and to what extent it contributes to body weight change in primary hyperparathyroidism (PHPT). Methods: Using the adeno-associated virus injection, Pth gene over-expressed mice mimicking PHPT were firstly established to observe their WAT browning and body weight alteration. The association between PTH and body weight was investigated in 496 PHPT patients. The adipose browning activities of 20 PHPT and 60 control subjects were measured with PET/CT scanning. Findings: Elevated plasma PTH triggered adipose tissue browning, leading to increased energy expenditure, reduced fat content, and finally decreased body weight in PHPT mice. Higher circulating PTH levels were associated with lower body weight (β = −0.048, P = .0003) independent of renal function, serum calcium, phosphorus,and albumin levels in PHPT patients. PHPT patients exhibited both higher prevalence of detectable brown/beige adipose tissue (20% vs 3.3%, P = .03) and increased browning activities (SUV in cervical adipose was 0.77 vs 0.49,P = .02) compared with control subjects. Interpretation: Elevated serum PTH drove WAT browning program, which contributed in part to body weight loss in both PHPT mice and patients. These results give insights into the novel pathological effect of PTH and are of importance in understanding the metabolic changes of PHPT. Fund: This research is supported by the National Key Research and Development Program of China and National Natural Science Foundation of China. Keywords: Primary hyperparathyroidism, Parathyroid hormone, Adipose tissue browning, Body weight
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- 2019
230. Engagement of cellular cholesterol in the life cycle of classical swine fever virus: its potential as an antiviral target
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Su Li, Yongfeng Li, Jinghan Wang, Lian-Feng Li, Yuan Sun, Caixia Yin, Shaoxiong Yu, Yuzi Luo, Hua-Ji Qiu, Kun Song, and Guang-Lai Zheng
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0301 basic medicine ,Swine ,media_common.quotation_subject ,030106 microbiology ,Regulator ,Antiviral Agents ,Virus ,Cell Line ,03 medical and health sciences ,chemistry.chemical_compound ,Flaviviridae ,Biosynthesis ,Virology ,Animals ,Internalization ,media_common ,biology ,Cholesterol ,beta-Cyclodextrins ,Pestivirus ,Epithelial Cells ,Virus Internalization ,biology.organism_classification ,Hydroxycholesterols ,030104 developmental biology ,chemistry ,Classical Swine Fever Virus ,Classical swine fever ,lipids (amino acids, peptides, and proteins) - Abstract
Classical swine fever virus (CSFV), the etiological agent of classical swine fever in pigs, is a member of the Pestivirus genus within the Flaviviridae family. It has been proposed that CSFV infection is significantly inhibited by methyl-β-cyclodextrin (MβCD) treatment. However, the exact engagement of cellular cholesterol in the life cycle of CSFV remains unclear. Here, we demonstrated that pretreatment of PK-15 cells with MβCD significantly decreased the cellular cholesterol level and resulted in the inhibition of CSFV infection, while replenishment of exogenous cholesterol in MβCD-treated cells recovered the cellular cholesterol level and restored the viral infection. Moreover, we found that depletion of cholesterol acted on the early stage of CSFV infection and blocked its internalization into the host cells. Furthermore, we showed that 25-hydroxycholesterol, a regulator of cellular cholesterol biosynthesis, exhibited a potent anti-CSFV activity by reducing cellular cholesterol level. Taken together, our findings highlight the engagement of cholesterol in the life cycle of CSFV and its potential use as an antiviral target.
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- 2019
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231. RailNet: A Segmentation Network for Railroad Detection
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Yu Hen Hu, Yin Wang, Ji Qiu, and Lide Wang
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General Computer Science ,Computer science ,Railroad detection ,Feature extraction ,ComputingMethodologies_IMAGEPROCESSINGANDCOMPUTERVISION ,ComputerApplications_COMPUTERSINOTHERSYSTEMS ,02 engineering and technology ,Pyramid ,0202 electrical engineering, electronic engineering, information engineering ,General Materials Science ,Computer vision ,Segmentation ,Pyramid (image processing) ,Electrical and Electronic Engineering ,business.industry ,Deep learning ,segmentation ,Frame (networking) ,General Engineering ,deep learning ,020207 software engineering ,Frame rate ,020201 artificial intelligence & image processing ,lcsh:Electrical engineering. Electronics. Nuclear engineering ,Artificial intelligence ,business ,lcsh:TK1-9971 - Abstract
Future trains will use more computer vision aids to help achieve fully autonomous driving. One of the most important parts of the train's visual function is the detection of railroad obstacles. This makes it important to identify and segment the railroad region within each video frame as it allows the train to identify the driving area so that it can do effective obstacle detection. Traditional railroad detection methods rely on hand-crafted features or highly specialized equipment such as lidar, which typically require expensive equipment to be maintained and are less reliable in scene changes. RailNet is a deep learning segmentation algorithm for railroad detection for videos captured by the front-view on-board cameras. RailNet provides an end-to-end solution that combines feature extraction and segmentation. We have modified the backbone network to extract multi-convolution features and use a pyramid structure to make the features have a top-to-bottom propagation. Our model can detect the railroad without generating large numbers of regions, which greatly increases the detection speed. Tested on a railroad segmentation dataset (RSDS) which we have built, RailNet exhibits very good performance while achieving 20 frames per second processing speed.
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- 2019
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232. The 24.5-kb Left Variable Region Is Not a Determinant for African Swine Fever Virus to Replicate in Primary Porcine Alveolar Macrophages
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Rui, Luo, Tao, Wang, Maowen, Sun, Li, Pan, Shujian, Huang, Yun, Sun, and Hua-Ji, Qiu
- Subjects
Infectious Diseases ,Virulence ,Swine ,Virology ,Macrophages, Alveolar ,Animals ,African Swine Fever ,African Swine Fever Virus ,Cells, Cultured - Abstract
African swine fever (ASF) is a widespread hemorrhagic and highly contagious infectious disease caused by African swine fever virus (ASFV), currently threatening the pig industry worldwide. Here, we demonstrated that the cell-adapted strain ASFV-P121 with a 24.5-kb deletion in the left variable region (LVR) lost the ability to replicate in primary porcine alveolar macrophages (PAMs). To explore whether this deletion determines the inability of ASFV-P121 replication in PAMs, a mutant virus (ASFV-ΔLVR) with the same LVR deletion as ASFV-P121 was constructed based on the wild-type ASFV HLJ/18 (ASFV-WT). However, the growth titer of ASFV-ΔLVR only reduced 10-fold compared with ASFV-WT in PAMs. Furthermore, we found that the large deletion of the LVR does not affect the formation of virus factories and virion morphogenesis. These findings reveal important implications for analyzing the molecular mechanism of ASFV cell tropism change.
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- 2022
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233. Antibody-Dependent Enhancement: ″Evil″ Antibodies Favorable for Viral Infections
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Xiaoke, Yang, Xin, Zhang, Xiaotian, Zhao, Mengqi, Yuan, Kehui, Zhang, Jingwen, Dai, Xiangyu, Guan, Hua-Ji, Qiu, and Yongfeng, Li
- Subjects
Infectious Diseases ,Virus Diseases ,Virology ,Viruses ,COVID-19 ,Humans ,Receptors, Fc ,Antibodies, Viral ,Antibodies, Neutralizing ,Antibody-Dependent Enhancement - Abstract
The pandemics caused by emerging viruses such as severe acute respiratory syndrome coronavirus 2 result in severe disruptions to public health. Vaccines and antibody drugs play essential roles in the control and prevention of emerging infectious diseases. However, in contrast with the neutralizing antibodies (NAbs), sub- or non-NAbs may facilitate the virus to enter the cells and enhance viral infection, which is termed antibody-dependent enhancement (ADE). The ADE of most virus infections is mediated by the Fc receptors (FcRs) expressed on the myeloid cells, while others are developed by other mechanisms, such as complement receptor-mediated ADE. In this review, we comprehensively analyzed the characteristics of the viruses inducing FcRs-mediated ADE and the new molecular mechanisms of ADE involved in the virus entry, immune response, and transcription modulation, which will provide insights into viral pathogenicity and the development of safer vaccines and effective antibody drugs against the emerging viruses inducing ADE.
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- 2022
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234. Safety and Efficacy of Transcatheter Closure of Atrial Septal Defects Guided by Transthoracic Echocardiography: A Prospective Study From Two Chinese Medical Centers
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Li, Gui-Shuang, Kong, Guang-Ming, Wang, Yu-Lin, Jin, You-Peng, Ji, Qiu-Shang, Li, Ji-Fu, You, Bei-An, and Zhang, Yun
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- 2009
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235. Preparation and characteristics of novel porous hydrogel films based on chitosan and glycerophosphate
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Zhao, Qing Sheng, Ji, Qiu Xia, Xing, Ke, Li, Xiao Yan, Liu, Cheng Sheng, and Chen, Xi Guang
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- 2009
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236. In vitro evaluation of the biomedical properties of chitosan and quaternized chitosan for dental applications
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Ji, Qiu Xia, Zhong, De Yu, Lü, Rui, Zhang, Wen Qing, Deng, Jing, and Chen, Xi Guang
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- 2009
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237. Identification of Antibody Biomarker Using High-Density Nucleic Acid Programmable Protein Array
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Lusheng, Song, Peter, Wiktor, Ji, Qiu, and Joshua, LaBaer
- Subjects
Protein Array Analysis ,Humans ,Antibodies ,Biomarkers - Abstract
A novel protein microarray technology, called high-density nucleic acid programmable protein array (HD-NAPPA), enables the serological screening of thousands of proteins at one time. HD-NAPPA extends the capabilities of NAPPA, which produces protein microarrays on a conventional glass microscope slide. By comparison, HD-NAPPA displays proteins in over 10,000 nanowells etched in a silicon slide. Proteins on HD-NAPPA are expressed in the individual isolated nanowells, via in vitro transcription and translation (IVTT), without any diffusion during incubation. Here we describe the method for antibody biomarker identification using HD-NAPPA, including four main steps: (1) HD-NAPPA array protein expression, (2) primary antibodies (serum/plasma) probing, (3) secondary antibody visualization, and (4) image scanning and data processing.
- Published
- 2021
238. Proteolytic activities of combined fermentation with Lactobacillus helveticus KLDS 1.8701 and Lactobacillus plantarum KLDS 1.0386 reduce antigenic response to cow milk proteins
- Author
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Ji Qiu, Shengnan Liang, Smith Etareri Evivie, Qinggang Xie, Fengyi Shi, Bailiang Li, Qingxue Chen, Guicheng Huo, and Lina Zhao
- Subjects
Antigenicity ,Proteases ,food.ingredient ,Tandem mass spectrometry ,Epitope ,fluids and secretions ,food ,Skimmed milk ,Genetics ,Animals ,Food science ,Lactobacillus helveticus ,biology ,Chemistry ,food and beverages ,Allergens ,biology.organism_classification ,Milk Proteins ,Fermentation ,Animal Science and Zoology ,Cattle ,Female ,Lactobacillus plantarum ,Food Science - Abstract
Cow milk protein is one of the leading food allergens. This study aimed to develop an effective method for reducing milk sensitization by evaluating antigenicity of fermented skim milk protein using Lactobacillus helveticus KLDS 1.8701, Lactobacillus plantarum KLDS 1.0386, and a combination of both strains. The proteolytic systems of strains in terms of genotype and phenotype are characterized by complete genome sequence, and evaluation the antigenicity of skim milk proteins was determined by ELISA and liquid chromatography with tandem mass spectrometry. Our results showed that the genomes encoded a variety of peptidase genes. For fermented skim milk, the degree of hydrolysis of the combined strains was higher than that of individual strain. Electrophoresis showed that the band color density of α-casein (α-CN) by fermentation of the combined strains was reduced when compared with control group. The fermentation process of the combined strains inhibited α-CN, β-lactoglobulin, and α-lactalbumin antigenicity by 69.13, 36.10, and 20.92, respectively. Major allergic epitopes of α-CN and β-lactoglobulin were cleaved by abundant proteases of combined strains. In all, this study showed that the fermentation process involving both L. helveticus and L. plantarum strains could reduce cow milk protein allergenicity through the combination of cell-envelope proteinase and peptidase on α-CN.
- Published
- 2021
239. Author response for 'Lgr4 promotes aerobic glycolysis and differentiation in osteoblasts via the canonical Wnt/β‐catenin pathway'
- Author
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Ji‐Qiu Wang, Yu-ying Yang, Jing-zun Xu, Jian‐Min Liu, Li-hao Sun, Yanman Zhou, Hong-yan Zhao, Bei Tao, and Weiqing Wang
- Subjects
Chemistry ,Anaerobic glycolysis ,Catenin ,Wnt signaling pathway ,Cell biology - Published
- 2021
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240. Genotyping and Molecular Characterization of Classical Swine Fever Virus Isolated in China during 2016–2018
- Author
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Yuan Sun, Yun-Juan Bao, Yongfeng Li, Su Li, Madiha Fatima, Peng-Ying Wang, Li Zhang, Yuzi Luo, Hua-Ji Qiu, Hao Song, and Yanhui Fu
- Subjects
0301 basic medicine ,China ,Genotype ,Swine ,030106 microbiology ,lcsh:QR1-502 ,subgenotype 2.1 ,Genome, Viral ,classical swine fever virus ,Neutralization ,Virus ,lcsh:Microbiology ,Article ,Classical Swine Fever ,molecular characterization ,03 medical and health sciences ,chemistry.chemical_compound ,Viral Envelope Proteins ,Virology ,Animals ,Amino Acid Sequence ,NS5B ,Genotyping ,Phylogeny ,Attenuated vaccine ,biology ,Genetic Variation ,Viral Vaccines ,biology.organism_classification ,030104 developmental biology ,Infectious Diseases ,chemistry ,Classical swine fever ,genetic typing ,antigenicity ,biology.protein ,Antibody - Abstract
Classical swine fever (CSF) is a highly contagious disease of swine caused by classical swine fever virus (CSFV). For decades the disease has been controlled in China by a modified live vaccine (C-strain) of genotype 1. The emergent genotype 2 strains have become predominant in China in the past years that are genetically distant from the vaccine strain. Here, we aimed to evaluate the current infectious status of CSF, and for this purpose 24 isolates of CSFV were identified from different areas of China during 2016–2018. Phylogenetic analysis of NS5B, E2 and full genome revealed that the new isolates were clustered into subgenotype 2.1d and 2.1b, while subgenotype 2.1d was predominant. Moreover, E2 and Erns displayed multiple variations in neutralizing epitope regions. Furthermore, the new isolates exhibited capacity to escape C-strain-derived antibody neutralization compared with the Shimen strain (genotype 1). Potential positive selection sites were identified in antigenic regions of E2 and Erns, which are related with antibody binding affinity. Recombination events were predicted in the new isolates with vaccine strains in the E2 gene region. In conclusion, the new isolates showed molecular variations and antigenic alterations, which provide evidence for the emergence of vaccine-escaping mutants and emphasize the need of updated strategies for CSF control.
- Published
- 2021
241. Construction of CoNi2S4 nanocubes interlinked by few-layer Ti3C2Tx MXene with high performance for asymmetric supercapacitors.
- Author
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Qi, Ji-Qiu, Huang, Meng-Yuan, Ruan, Chen-Ya, Zhu, Dong-Dong, Zhu, Lei, Wei, Fu-Xiang, Sui, Yan-Wei, and Meng, Qing-Kun
- Abstract
Copyright of Rare Metals is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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- 2022
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242. Differences in proteomic profiles between yak and three cattle strains provide insights into molecular mechanisms underlying high‐altitude adaptation
- Author
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Xin, Jin‐Wei, primary, Chai, Zhi‐Xin, additional, Zhang, Cheng‐Fu, additional, Zhang, Qiang, additional, Zhu, Yong, additional, Cao, Han‐Wen, additional, YangJi, Cidan, additional, Chen, Xiao‐Ying, additional, Jiang, Hui, additional, Zhong, Jin‐Cheng, additional, and Ji, Qiu‐Mei, additional
- Published
- 2021
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243. The genetic variation landscape of African swine fever virus reveals frequent positive selection and adaptive flexibility
- Author
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Fernando Rodriguez, Yun-Juan Bao, Yuzi Luo, Hua-Ji Qiu, Junhui Qiu, Producció Animal, and Sanitat Animal
- Subjects
Swine ,040301 veterinary sciences ,Population ,African swine fever virus ,0403 veterinary science ,Viral Proteins ,03 medical and health sciences ,Genetic variation ,Animals ,Genetic variability ,African Swine Fever ,education ,Selection (genetic algorithm) ,030304 developmental biology ,Swine Diseases ,0303 health sciences ,education.field_of_study ,General Veterinary ,General Immunology and Microbiology ,biology ,Genetic Variation ,04 agricultural and veterinary sciences ,General Medicine ,biology.organism_classification ,African Swine Fever Virus ,Evolutionary biology ,Host adaptation ,Adaptation ,Selective sweep - Abstract
African swine fever virus (ASFV) is a lethal disease agent that causes high mortality in swine population and devastating loss in swine industries. The development of efficacious vaccines has been hindered by the gap in knowledge concerning genetic variation of ASFV and the genetic factors involved in host adaptation and virus-host interactions. In this study, we performed a meta-genetic study of ASFV aiming to profile the variation landscape and identify genetic factors with signatures of positive selection and relevance to host adaptation. Our data reveals a high level of genetic variability of ASFV shaped by both diversifying selection and selective sweep. The selection signatures are widely distributed across the genome with the diversifying selection falling within 29 genes and selection sweep within 25 genes, highlighting strong signals of adaptive evolution of ASFV. Further examination of the sequence properties reveals the link of the selection signatures with virus-host interactions and adaptive flexibility. Specifically, we discovered a site at 157th of the key antigen protein EP402R under diversifying selection, which is located in the cytotoxic T-cell epitope related with the low level of cross-reaction in T-cell response. Importantly, two multigene families MGF360 and MGF505, the host range factors of ASFV, exhibit divergent selection among the paralogous members, conferring sequence pools for genetic diversification and adaptive capability. By integrating the genes with selection signatures into a unified framework of interactions between ASFV and hosts, we showed that the genes are involved in multiple processes of host immune interaction and virus life cycles, and may play crucial roles in circumventing host defense systems and enhancing adaptive fitness. Our findings will allow enhanced understanding of genetic basis of rapid spreading and adaptation of ASFV among the hosts.
- Published
- 2021
244. Identification of Antibody Biomarker Using High-Density Nucleic Acid Programmable Protein Array
- Author
-
Ji Qiu, Peter Wiktor, Joshua LaBaer, and Lusheng Song
- Subjects
0303 health sciences ,biology ,Chemistry ,030302 biochemistry & molecular biology ,Microscope slide ,Translation (biology) ,Primary and secondary antibodies ,Biomarker (cell) ,03 medical and health sciences ,Biochemistry ,Gene chip analysis ,biology.protein ,Nucleic acid ,Protein microarray ,Antibody ,030304 developmental biology - Abstract
A novel protein microarray technology, called high-density nucleic acid programmable protein array (HD-NAPPA), enables the serological screening of thousands of proteins at one time. HD-NAPPA extends the capabilities of NAPPA, which produces protein microarrays on a conventional glass microscope slide. By comparison, HD-NAPPA displays proteins in over 10,000 nanowells etched in a silicon slide. Proteins on HD-NAPPA are expressed in the individual isolated nanowells, via in vitro transcription and translation (IVTT), without any diffusion during incubation. Here we describe the method for antibody biomarker identification using HD-NAPPA, including four main steps: (1) HD-NAPPA array protein expression, (2) primary antibodies (serum/plasma) probing, (3) secondary antibody visualization, and (4) image scanning and data processing.
- Published
- 2021
- Full Text
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245. A Strain Rate‐Dependent Constitutive Model for Asymmetric Hardening Behavior of TB9 Titanium Alloy
- Author
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Xiaochao Jin, Qipeng Xu, Ji Qiu, Tao Jin, Pan Li, Cheng Hou, Le Liu, and Xueling Fan
- Subjects
General Materials Science ,Condensed Matter Physics - Published
- 2022
- Full Text
- View/download PDF
246. Serum autoantibodyome reveals that healthy individuals share common autoantibodies
- Author
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Mahasish Shome, Yunro Chung, Ramani Chavan, Jin G. Park, Ji Qiu, and Joshua LaBaer
- Subjects
Serum ,Adolescent ,Protein Array Analysis ,Humans ,Autoantigens ,General Biochemistry, Genetics and Molecular Biology ,Autoantibodies ,Autoimmune Diseases - Abstract
Autoantibodies are a hallmark of both autoimmune disease and cancer, but they also occur in healthy individuals. Here, we perform a meta-analysis of nine datasets and focus on the common autoantibodies shared by healthy individuals. We report 77 common autoantibodies based on the protein microarray data obtained from probing 182 healthy individual sera on 7,653 human proteins and an additional 90 healthy individual sera on 1,666 human proteins. There is no gender bias; however, the number of autoantibodies increase with age, plateauing around adolescence. We use a bioinformatics pipeline to determine possible molecular-mimicry peptides that can contribute to the elicitation of these common autoantibodies. There is enrichment of intrinsic properties of proteins like hydrophilicity, basicity, aromaticity, and flexibility for common autoantigens. Subcellular localization and tissue-expression analysis reveal that several common autoantigens are sequestered from the circulating autoantibodies.
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- 2022
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247. A novel injectable chlorhexidine thermosensitive hydrogel for periodontal application: preparation, antibacterial activity and toxicity evaluation
- Author
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Ji, Qiu Xia, Zhao, Qing Sheng, Deng, Jing, and Lü, Rui
- Published
- 2010
- Full Text
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248. Preparation of alginate coated chitosan hydrogel beads by thermosensitive internal gelation technique
- Author
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Zhao, Qing Sheng, Ji, Qiu Xia, Cheng, Xiao Jie, Sun, Gang Zheng, Ran, Chao, Zhao, Bing, and Chen, Xi Guang
- Published
- 2010
- Full Text
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249. Injectable thermosensitive hydrogel based on chitosan and quaternized chitosan and the biomedical properties
- Author
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Ji, Qiu Xia, Chen, Xi Guang, Zhao, Qing Sheng, Liu, Cheng Sheng, Cheng, Xiao Jie, and Wang, Ling Chong
- Published
- 2009
- Full Text
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250. Effect of organic and inorganic acids on chitosan/glycerophosphate thermosensitive hydrogel
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Zhao, Qing Sheng, Cheng, Xiao Jie, Ji, Qiu Xia, Kang, Chuan Zhen, and Chen, Xi Guang
- Published
- 2009
- Full Text
- View/download PDF
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