242 results on '"Huiling Guo"'
Search Results
202. Re-sensitization of radiation resistant colorectal cancer cells to radiation through inhibition of AMPK pathway
- Author
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Hongyong Jin, Huiling Guo, Sujie Gao, Shengnan Ren, Fujian Ji, Xuebo Chen, and Zhuo Liu
- Subjects
0301 basic medicine ,Cancer Research ,medicine.medical_specialty ,Small interfering RNA ,Tumor suppressor gene ,Oncogene ,Colorectal cancer ,AMPK ,Cancer ,Articles ,Biology ,medicine.disease ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,Endocrinology ,Oncology ,030220 oncology & carcinogenesis ,Radioresistance ,Internal medicine ,Cancer cell ,medicine ,Cancer research - Abstract
Radiotherapy (RT) is commonly used to treat multi-tumors to attenuate the risk of recurrence. Despite impressive initial clinical responses, a large proportion of patients experience resistance to RT. Therefore, identification of functionally relevant biomarkers would be beneficial for radioresistant patients. Adenosine monophosphate-activated kinase (AMPK) is recognized as a mediator of tumor suppressor gene function. In the present study, radio-sensitive and -resistant colon cancer patient samples were compared and the AMPK pathway was observed to be highly activated in radioresistant patients. In addition, the protein and mRNA levels of AMPK were upregulated in radioresistant colon cancer cells in comparison to radiosensitive colon cancer cells. The present study provides evidence that activation of AMPK by metformin contributes to radioresistance. Inhibition of AMPK by either small interfering RNA or Compound C, which is a specific inhibitor of AMPK, re-sensitized radiation resistant cells. The data presented indicates a synergistic effect on radiation resistant cancer cells by the combination of Compound C and radiation. In summary, the present study proposes that inhibition of the AMPK pathway is a potential strategy for reversing radiation resistance and may contribute to the development of therapeutic anticancer drugs.
- Published
- 2015
203. Research on the Classification of Image Semantic Scene
- Author
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Lingshan Jia, Fan Zhang, and Huiling Guo
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Vocabulary ,Pixel ,Computer science ,business.industry ,media_common.quotation_subject ,ComputingMethodologies_IMAGEPROCESSINGANDCOMPUTERVISION ,Boundary (topology) ,Pattern recognition ,Grid ,Image (mathematics) ,Categorization ,Computer Science::Computer Vision and Pattern Recognition ,Computer vision ,Artificial intelligence ,Cluster analysis ,business ,Word (computer architecture) ,media_common - Abstract
This paper mainly aims at the algorithm of scene categorization. Firstly, putting forward by using the edge information of training set image to modela probabilistic generative model, and getingt the shape prior distribution of each scene category by learning, and inferencing to get the boundary distribution image,so to finish the regular super pixel grid block.Then, clustering the local word package description data of sub region, to construct the semantic visual vocabulary SVV, combining with the regularized super pixel grid to descript image; finally, finishing the task.
- Published
- 2015
204. Construction of management system on wireless sensor network protocol based on knowledge data association mining technology
- Author
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HuiLing Guo and QiuFen Wang
- Subjects
Routing protocol ,Engineering ,Association rule learning ,Data stream mining ,business.industry ,Wireless Routing Protocol ,computer.software_genre ,Network management application ,Key distribution in wireless sensor networks ,Knowledge extraction ,Data mining ,business ,Wireless sensor network ,computer ,Computer network - Abstract
Data mining is a very important step of knowledge discovery and it is through the establishment of mining model and through the implementation of the corresponding algorithm to complete knowledge formation. This paper analyzes the advantages and disadvantages of the management information system of wireless sensor networks protocols. Data mining tools can find association rules that satisfy the condition. The paper presents constructing of information management system on wireless sensor network protocol based on knowledge data association mining. Experimental results show that the proposed method can improve the efficiency of routing protocol.
- Published
- 2015
205. Analysis of Knowledge Data Discovery and Mining by Construction of Natural Language Understanding System
- Author
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Huiling Guo and Qiufen Wang
- Subjects
Parsing ,Universal Networking Language ,Knowledge extraction ,Computer science ,Natural language understanding ,Question answering ,Software mining ,Data discovery ,computer.software_genre ,computer ,Data science ,Natural language - Abstract
Natural language understanding mainly studies the language communicative process simulation of people with electronic computer, and the computer is able to understand and use the natural language of the human society. This paper analyzes the basic components and functions of knowle dge discovery and data mining application system. The paper presents analysis of knowledge da ta discovery and mining by construction of natural language understanding system. The e xperimental results show that this method can improve efficiency of knowledge discovery system.
- Published
- 2015
206. Biofilm Formation Characteristics of Bacterial Isolates Retrieved from a Reverse Osmosis Membrane
- Author
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Chee Meng Pang, Huiling Guo, Pei-Ying Hong, and Wen Tso Liu
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Microscopy, Confocal ,Surface Properties ,Movement ,Biofilm ,Motility ,Membranes, Artificial ,Portable water purification ,General Chemistry ,Biology ,Bacterial Physiological Phenomena ,Microscopy, Atomic Force ,Water Purification ,Microbiology ,Membrane technology ,Biofouling ,Membrane ,Species Specificity ,Cell Wall ,Biofilms ,Environmental Chemistry ,Water treatment ,Reverse osmosis ,In Situ Hybridization, Fluorescence - Abstract
High-quality water purification systems using reverse osmosis (RO) membrane separation have faced a major challenge related to biofilm formation on the membrane surface, or biofouling. To understand this issue, the biofilm formation characteristics of four bacterial isolates previously retrieved from an RO membrane treating potable water were investigated. Biofilm formation of all four isolates occurred to different extents in microtiter plates and could be related to one or more cell properties (hydrophobicity, surface charge, and motility). For Dermacoccus sp. strain RO12 and Microbacterium sp. strain RO18, bacterial adhesion was facilitated by cell surface hydrophobicity, and for Rhodopseudomonas sp. strain RO3, adhesion was assisted by its low surface charge. Sphingomonas sp. strain RO2 possessed both twitching and swarming motilities, which could be important in mediating surface colonization. Further, strains RO2, RO3, and RO12 did not exhibit swimming motility, suggesting that they could be transported to RO membrane surfaces by other mechanisms such as convective permeate flow. The biofilm formation of RO2 was further tested on different RO membranes made of cellulose acetate, polyamide, and thin film composite in continuous flow cell systems. The resultant RO2 biofilms were independent of membrane surface properties and this was probably related to the ex-opolysaccharides secreted bythe biofilm cells. These results suggested that RO2 could colonize RO membranes effectively and could be a potential fouling organism in RO membranes for freshwater purification.
- Published
- 2005
207. Synthesis and characterization of thiol-functionalized mesoporous silica nanoparticles
- Author
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Zhifei Xie, Mingxing Liu, Hongda Zhu, Huiling Guo, Honghao Sun, and Lingling Huang
- Published
- 2014
208. MiR-29a/b/c regulate human circadian gene hPER1 expression by targeting its 3'UTR
- Author
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Xiyan, Zhao, Xueqiang, Zhu, Shuting, Cheng, Yizhou, Xie, Zhengrong, Wang, Yanyou, Liu, Zhou, Jiang, Jing, Xiao, Huiling, Guo, and Yuhui, Wang
- Subjects
MicroRNAs ,Base Sequence ,Reverse Transcriptase Polymerase Chain Reaction ,Cell Line, Tumor ,Blotting, Western ,Down-Regulation ,Humans ,Period Circadian Proteins ,3' Untranslated Regions ,Algorithms ,DNA Primers - Abstract
Several essential biological progresses in mammals are regulated by circadian rhythms. Though the molecular mechanisms of oscillating these circadian rhythms have been uncovered, the specific functions of the circadian genes are not very clear. It has been reported that knocking down circadian genes by microRNA is a useful strategy to explore the function of the circadian rhythms. In this study, through a forward bioinformatics screening approach, we identified miR-29a/b/c as potent inhibitors for the human circadian gene hPER1. We further found that miR-29a/b/c could directly target hPER1 3'untranslated region (UTR) and down-regulate hPER1 at both mRNA and protein expression levels in human A549 cells. Thus, our findings suggested that the expression of hPER1 is regulated by miR-29a/b/c, which may also provide a new clue for the function of hPER1.
- Published
- 2014
209. A combination of insulin and ubiquitin A20 promotes osteocalcin expression in adipose-derived stem cells
- Author
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Huiling Guo, Xia Xu, Hongchen Liu, Na Huo, Lin Wang, and Dongshen Wang
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medicine.medical_specialty ,medicine.medical_treatment ,Osteocalcin ,Primary Cell Culture ,bcl-X Protein ,Adipose tissue ,Apoptosis ,Core Binding Factor Alpha 1 Subunit ,Biology ,Transfection ,Biochemistry ,Receptor, IGF Type 1 ,hemic and lymphatic diseases ,Internal medicine ,medicine ,Adipocytes ,Humans ,Insulin ,Molecular Biology ,Tumor Necrosis Factor alpha-Induced Protein 3 ,bcl-2-Associated X Protein ,Stem Cells ,Intracellular Signaling Peptides and Proteins ,Nuclear Proteins ,Cell Biology ,RUNX2 ,DNA-Binding Proteins ,Endocrinology ,medicine.anatomical_structure ,Adipose Tissue ,Gene Expression Regulation ,Osteocyte ,biology.protein ,Stem cell ,Signal Transduction - Abstract
Osteocyte generation can be used in bone defect repair; the generation efficiency needs to be further improved. This study aims to evaluate the role of ubiquitin A20 (A20) in facilitating the expression of osteocalcin in adipose-derived stem cells (ADSCs). In this study, adipose tissue was obtained from 10 healthy human subjects; ADSCs were isolated from the adipose samples. The ADSCs were transfected with core binding factor alpha 1 (Cbfa1) and/or insulin-like growth factor-1 receptor (IGF-1R). Expression of osteocalcin, A20 in ADSCs was assessed by quantitative RT-PCR (qRT-PCR) and Western blotting. Apoptosis of ADSCs was analyzed by flow cytometry. The results showed that after the gene transfection and stimulation of insulin, the ADSCs expressed high levels of osteocalcin. However, apoptotic ADSCs were induced by the activation of IGF-1R. Exposure to insulin down-regulated the expression of Bcl-xL and A20, and increased Bax, in ADSCs. The addition of exogenous A20 prevented the ADSC apoptosis. We conclude that activation of IGF-1R can induce apoptosis in ADSCs, which can be prevented by addition of exogenous A20.
- Published
- 2013
210. Research on the architecture of database intrusion protection system in e-commerce environment
- Author
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Huiling Guo
- Subjects
Engineering ,Database ,business.industry ,Intrusion detection system ,Active database ,Database application ,System monitoring ,Computer security ,computer.software_genre ,Security controls ,Host-based intrusion detection system ,business ,Database security ,computer ,Protection mechanism - Abstract
Security has been a critical issue in e-commerce, where absolute security confidentiality of information exchange is requited. Because of the shortage of traditional database security protection mechanism, an active database intrusion prevention system is proposed to monitor the database application layer activities. The architecture of database intrusion prevention system is designed, including a security control module, intrusion detection module, audit and configuration module as well as alarm module, which could be applied in e-commerce applications.
- Published
- 2013
211. Tryptase compromises corneal epithelial barrier function
- Author
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Qiyan, Li, Ying, Jie, Cong, Wang, Yingnan, Zhang, Huiling, Guo, and Zhiqiang, Pan
- Subjects
Matrix Metalloproteinase 9 ,Transcription, Genetic ,Epithelium, Corneal ,Humans ,Receptor, PAR-2 ,Tryptases ,Endosomes ,Gene Silencing ,Matrix Metalloproteinase Inhibitors ,Lysosomes ,Membrane Fusion ,Cell Line - Abstract
Corneal epithelial barrier dysfunction is harmful to corneal health; the pathogenesis is unclear. This study aims to elucidate the mechanism by which tryptase compromises corneal epithelial barrier function. Human corneal epithelial cell line (HCE cells) was cultured into monolayers using as a study platform. Quantitative reverse transcription polymerase chain reaction and Western blotting were employed to detect the expression of matrix metalloprotenases (MMP)9. The endosome/lysosome fusion was observed by confocal microscopy. The corneal epithelial barrier function was assessed in Transwell system. The results showed that HCE cells expressed proteinase-activated receptor (PAR)2. Activation of PAR2 by tryptase induced expression of MMP9 in HCE cells, interfered with the fusion of endosome/lysosome, and compromised the epithelial barrier function, which could be prevented by pretreatment with MMP9 inhibitor. We conclude that tryptase can increase the expression of MMP9 in HCE cells and compromise the epithelial barrier function.
- Published
- 2013
212. Integrin alpha v beta 6 contributes to maintaining corneal epithelial barrier function
- Author
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Huiling, Guo, Gaiping, Du, Liqiang, Wang, Dajiang, Wang, Lianna, Hu, and Yifei, Huang
- Subjects
Integrins ,Antigens, Neoplasm ,Tumor Necrosis Factor-alpha ,Epithelium, Corneal ,Humans ,Epithelial Cells ,Endosomes ,Immunohistochemistry - Abstract
The corneal epithelial barrier dysfunction is associated with a number of disorders of the cornea. This study aims to investigate the role of integrin alpha v beta 6 (avb6) in maintaining the corneal epithelial barrier function. In this study, the association of avb6 and endosome/lysosome in the human corneal epithelial cell line, HCE cells, was observed with immunocytochemistry. The corneal epithelial barrier function was assessed with HCE monolayers in Transwells. The results showed that avb6 was observed attaching to endosomes in HCE cells. Knockdown of avb6 resulted in disturbing the fusion of endosome/lysosome. Tumor necrosis factor (TNF)-α could bind avb6 to form a complex that interfered with the fusion of endosome/lysosome in HCE cells. With the HCE monolayer as a corneal epithelial barrier model, the knockdown of avb6 or exposure to TNF-α markedly increased the epithelial barrier permeability to a macomolecular protein, ovalbumin, in Transwell system. After passing through the deficient epithelial barrier, the ovalbumin molecules still conserved the antigenicity. We conclude that the integrin avb6 plays an important role in the fusion of endosome/lysosome in corneal epithelial cells; inhibition of avb6 results in corneal epithelial barrier dysfunction.
- Published
- 2012
213. Mining schema matching between heterogeneous databases
- Author
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Wei Chen, Xiaowei Pu, Fang Zhang, Huiling Guo, and Xuefei Liu
- Subjects
Information retrieval ,Database ,Distributed database ,Computer science ,Schema (psychology) ,Key (cryptography) ,ComputerApplications_COMPUTERSINOTHERSYSTEMS ,Pattern matching ,Data mining ,computer.software_genre ,Schema matching ,computer ,Data integration - Abstract
Schema matching plays a key role in heterogeneous data sources integration which can find out semantic correspondences between elements of two schemas. In this paper, a schema matching algorithm named HDSM is proposed. It can discover both 1:1 and complex matches, and find mappings between opaque columns. Experiments prove that HDSM can not only discover matches between heterogeneous databases roundly, but also enhance the efficiency and precision of schema matching.
- Published
- 2012
214. A novel method for the Doppler-shift correction of the measure and control signal
- Author
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Huiling Guo, Fang Zhang, Wei Chen, Jia Liu, and Nannan Gai
- Subjects
Control theory ,Non-uniform discrete Fourier transform ,Frequency domain ,Short-time Fourier transform ,Frequency offset ,Spectral density estimation ,Spectral density ,Data_CODINGANDINFORMATIONTHEORY ,Algorithm ,Fractional Fourier transform ,Discrete Fourier transform ,Mathematics - Abstract
Because of the adverse effect of Doppler-shift (frequency offset) to the measure and control signal, this article describes a novel method for the correction of the Doppler-shift in the received signal based on Discrete Fourier Transform (DFT). It presents the results of computer-simulation tests to measure the time taken to correct the Doppler shift at a very low signal-noise ration.
- Published
- 2012
215. Performance analysis for selection combining in Rician environments
- Author
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Huiling Guo, Fang Zhang, Wei Chen, and Xuefei Liu
- Subjects
Channel correlation ,Rayleigh channels ,Computer science ,business.industry ,Data_CODINGANDINFORMATIONTHEORY ,Signal ,Computer Science::Performance ,Correlation ,Signal-to-noise ratio ,Rician fading ,Telecommunications ,business ,Algorithm ,Selection (genetic algorithm) ,Polarization diversity ,Computer Science::Information Theory - Abstract
This article analyzes the performance of selection combining in Rician fading environments. Introduces a S+N(signal-plus-noise) selection combining arithmetic compared with traditional SNR(signal to noise ratio) selection combining of polarization diversity combining of TT&C signal. The result of computer-simulation tells us that the capability of this arithmetic is better than that of SNR. Finally, simply analyzes the effect of channel correlation on the S + N selection combined in Rician fading environments, and gives the simulation results.
- Published
- 2011
216. Changes in objective vault and effect on vision outcomes after implantable Collamer lens implantation: 1-year follow-up
- Author
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Huiling Guo, Dajiang Wang, Liqiang Wang, Hou-Bing Huang, Gaiping Du, Jing Song, Jifeng Yu, Yu-Bo Gong, and Yifei Huang
- Subjects
Adult ,Male ,medicine.medical_specialty ,Phakic Intraocular Lenses ,genetic structures ,Adolescent ,Visual Acuity ,1 year follow up ,Phakic intraocular lens ,03 medical and health sciences ,Young Adult ,0302 clinical medicine ,Lens Implantation, Intraocular ,Lens, Crystalline ,Myopia ,Medicine ,Humans ,Retrospective Studies ,Implantable collamer lens ,business.industry ,Follow up studies ,Retrospective cohort study ,General Medicine ,Middle Aged ,humanities ,eye diseases ,Surgery ,Ophthalmology ,030221 ophthalmology & optometry ,Female ,sense organs ,business ,030217 neurology & neurosurgery ,Follow-Up Studies - Abstract
To investigate the changes in vault and the effect on visual outcomes 1 year after implantable Collamer lens (ICL) implantation.In this retrospective study, 127 eyes of 66 patients undergoing ICL implantation were examined both before and up to 1 year after the surgery. The examination contents included white-to-white (WTW) diameter, central vault of the ICL (distance between posterior surface of ICL and anterior surface of crystalline lens), refractive error, and wavefront high-order aberration (HOA). All statistical analyses were performed using SPSS 13.0 software.A significant decrease in vault was noted up to 1 month, after which the value stabilized (p=0.001). The moderate vault decreased significantly after the first 3 months postsurgery (paired-samples t test, p0.05). Low vault showed a tendency to increase and high vault showed a tendency to decrease, but not significantly, over time. There was no statistically significant correlation between the amount of vault and the refractive error (Pearson correlation coefficient R=0.111, p=0.473) and there was a statistically significant correlation between the vault and HOAs (R=0.304, p=0.024).Implantable Collamer lens vault over the crystalline lens had the tendency toward a slight decrease with time and did not significantly affect the vision outcome 1 year after surgery.
- Published
- 2011
217. Application of GPS on Power System Operation
- Author
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Bin He, Wei Liu, Chunmei Pei, Huiling Guo, Xiuqing Yang, and Xuemei Li
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Electric power system ,Computer science ,business.industry ,Information and Communications Technology ,Global Positioning System ,Electrical engineering ,Power-system automation ,Joint (building) ,business ,Power budget ,Power control ,Power (physics) - Abstract
Applying GPS positioning and navigation technology to power systems, will realize precise navigation of power equipments, improve power system automation of routine works, and enhance working efficiency. In case of emergency, rapid fixing arrangements can be implemented through monitoring and command platform. With advance communication technology, the realtime video can be transferred to the experts all over the world for remote joint consultation.
- Published
- 2011
218. Sensors in Smart Phone
- Author
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Huiling Guo, Yangqiu Wang, Chunmei Pei, Xiaojing Zhang, Xiuqing Yang, and Ye Hairong
- Subjects
Multimedia ,Mobile phone tracking ,SIMPLE (military communications protocol) ,Visual sensor network ,Computer science ,business.industry ,computer.software_genre ,Mobile phone ,Mobile station ,The Internet ,Electronics ,Image sensor ,business ,computer - Abstract
The technological innovation in electronics makes nowadays mobile phone more than a simple communication tool: it becomes a portable electronic device with integrated functions, such as listening to music, watching movies, taking photos, etc. To achieve these, many kinds of advanced sensor are used. In this paper, several applications of sensor in smart phone are introduced including Image Sensor, Fingerprint Identification Sensor, Photo-electric Sensor, Acceleration Sensor, etc. The trend of the mobile phone, 3I (Intellignet, Interaction and Internet), will have all kinds of sensor more and more popular in mobile phones to change our life.
- Published
- 2011
219. Inland Water Container Transportation Organization Mode of Middle-Grade Waterway Based on River-Sea Multimodal Transportation
- Author
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Renfa Yang and Huiling Guo
- Subjects
Transport engineering ,Engineering ,Water transport ,business.industry ,media_common.quotation_subject ,Mode (statistics) ,business ,Middle grades ,Civil engineering ,Adaptability ,media_common - Abstract
Based on the analysis of the current status of Chinese M, inland water container transportation development and relevant factors, the problem of poor timeliness was pointed out as the key restrictive factor to the container transportation. An innovative container transport organization mode of river-sea docking through transport is proposed to adapt to the middle-grade waterway and to satisfy the needs of trunk lines container transport. Applied result indicates that the mode can effectively reduce the relay link, enhance the transportation efficiency, guarantee the sailing schedule of trunk lines container and eliminate the discredits of cargo owner to inland water transportation speed. The mode has strong adaptability and maneuverability to develop middle-grade channel container transportation.
- Published
- 2010
220. [A Systematic Review of Erlotinib for Advanced Non-small Cell Lung Cancer.]
- Author
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Huiling, Guo, Rongfang, Zhang, Qimei, Yang, Lingjuan, Zhang, Kehu, Yang, and Jinhui, Tian
- Abstract
It was unclear whether advanced non-small cell lung cancer (NSCLC) patients could benefit from erlotinib therapy. This study was aimed to evaluate the efficacy and safety of NSCLC patients treated with erlotinib.The relevant randomized controlled trials (RCT) were searched from VIP, CMB, CNKI, PubMed, EMBASE and The Cochrane Library. The related references and experts in this field were traced, and other authors were communicated with to obtain the information that has not been found. Quality assessment of qualified RCTs assessed by the exclusion and inclusion criteria and RevMan 5.0 provided by the Cochrane Collaboration was used to perform meta-analysis.Three RCTs involving 2 969 patients were included. Meta analysis results suggested that erlotinib was superior to placebo for one year survival rate (OR=1.18, 95%CI: 1.01-1.38), tumor response rate (OR=1.24, 95%CI: 1.03-1.49), median overall survival, median progression-free survival and tumor responses duration. At the same time, the incidence of grade 3-4 skin rash (OR=16.33, 95%CI: 7.01-38.02) and diarrhea (OR=5.02, 95%CI: 2.93-8.60) of the adverse reactions was increased.Advanced non-small cell lung cancer patients could benefit from Erlotinib therapy, but the incidence of skin rash and diarrhea was significantly increased, and in the absence of damage to the blood system, serious liver and kidney damage, cardiac toxicity, etc, there were no difference with placebo.
- Published
- 2010
221. Axin determines cell fate by controlling the p53 activation threshold after DNA damage
- Author
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Zailian Lu, Ka Ruan, Huiling Guo, Shu-Yong Lin, Jiahuai Han, Guili Lian, Yanhai Wang, Sheng-Cai Lin, Zhiyun Ye, Xuan Wang, and Qinxi Li
- Subjects
DNA damage ,Ubiquitin-Protein Ligases ,Apoptosis ,Cell Cycle Proteins ,Genotoxic Stress ,Ataxia Telangiectasia Mutated Proteins ,Biology ,Cell fate determination ,Protein Serine-Threonine Kinases ,Lysine Acetyltransferase 5 ,law.invention ,Mice ,Axin Protein ,law ,Cell Line, Tumor ,Animals ,Humans ,Cell Lineage ,Histone Acetyltransferases ,chemistry.chemical_classification ,DNA ligase ,Papilloma ,Kinase ,Tumor Suppressor Proteins ,Wnt signaling pathway ,Cell Biology ,Cell biology ,DNA-Binding Proteins ,Repressor Proteins ,chemistry ,Acetyltransferase ,Checkpoint Kinase 1 ,Mutation ,Suppressor ,Tumor Suppressor Protein p53 ,Protein Kinases ,DNA Damage ,Protein Binding - Abstract
Cells can undergo either cell-cycle arrest or apoptosis after genotoxic stress, based on p53 activity(1-6). Here we show that cellular fate commitment depends on Axin forming distinct complexes with Pirh2, Tip60, HIPK2 and p53. In cells treated with sublethal doses of ultra-violet (UV) radiation or doxorubicin (Dox), Pirh2 abrogates Axin-induced p53 phosphorylation at Ser 46 catalysed by HIPK2, by competing with HIPK2 for binding to Axin. However, on lethal treatment, Tip60 interacts with Axin and abrogates Pirh2-Axin binding, forming an Axin-Tip60-HIPK2-p53 complex that allows maximal p53 activation to trigger apoptosis. We also provide evidence that the ATM/ATR pathway mediates the Axin-Tip60 complex assembly. An axin mutation promotes carcinogenesis in Axin(Fu)/+ (Axin-Fused) mice, consistent with a dominantnegative role for Axin(Fu) in p53 activation. Thus, Axin is a critical determinant in p53-dependent tumour suppression in which Pirh2 and Tip60 have different roles in triggering cell-cycle arrest or apoptosis depending on the severity of genotoxic stress.
- Published
- 2009
222. Association of the 54-nucleotide repeat polymorphism of hPer3 with heroin dependence in Han Chinese population
- Author
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Jing Xiao, Chaomin Wan, Yu-Zhong Wang, Z. Yang, Shiping Li, Huiling Guo, G. Liao, Y. Shen, Y. Zou, Yanyou Liu, Zheng Wang, and Y. Lin
- Subjects
Adult ,Male ,China ,Genotype ,Circadian clock ,Population genetics ,Biology ,Polymerase Chain Reaction ,Polymorphism, Single Nucleotide ,law.invention ,Behavioral Neuroscience ,Sex Factors ,Asian People ,Gene Frequency ,law ,Genetics ,Humans ,Circadian rhythm ,Allele ,Allele frequency ,Polymerase chain reaction ,Alleles ,Aged ,Chronobiology ,Heroin Dependence ,Nuclear Proteins ,Period Circadian Proteins ,Middle Aged ,Neurology ,Agarose gel electrophoresis ,Female ,Transcription Factors - Abstract
Circadian clock genes have the function of producing circadian rhythm. They are also implicated in the origin or development of many diseases such as cancers and neuropsychiatric diseases. The purpose of this study is to determine whether the 54-nucleotide repeat polymorphism of hPer3, one of the circadian clock genes, associates with heroin dependence. DNA samples were obtained from 209 Chinese heroin-dependent subjects and 249 Chinese healthy controls. The 54-nucleotide repeat polymorphism was detected by polymerase chain reaction and DNA agarose gel electrophoresis. The frequency of four-repeat allele was significantly higher (chi(2)= 10.64, P = 0.001; corrected for multiple tests, P = 0.003) in the mixed gender heroin-dependent subject group (four repeat: 0.89, five repeat: 0.11) than in the mixed gender control group (four repeat: 0.81, five repeat: 0.19); the frequency of four-repeat allele was also significantly higher (chi(2)= 10.00, P = 0.002; corrected for multiple tests, P = 0.006) in the male heroin-dependent subject group (four repeat: 0.89, five repeat: 0.11) than in the male control group (four repeat: 0.81, five repeat: 0.19); for females, no significant trend was observed with the 54-nucleotide repeat polymorphism between the heroin-dependent subject group and the control group. Our results suggest that the 54-nucleotide repeat polymorphism of hPer3 significantly associates with heroin dependence at the allele frequency level and may be a potential risk factor for the development of heroin dependence.
- Published
- 2007
223. [Effects of encapsulated plasmid recombining with hANP cDNA transfected cells on morphological and histological characteristic of experimental hypertensive rats]
- Author
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Haoyang, Xing, Liguo, Chen, Jing, Xiao, Huiling, Guo, Chaomin, Wan, and Zhengrong, Wang
- Subjects
Male ,Recombination, Genetic ,DNA, Complementary ,Cell Transplantation ,Capsules ,CHO Cells ,Genetic Therapy ,Kidney ,Transfection ,Rats ,Hypertension, Renovascular ,Cricetinae ,Animals ,Humans ,Transgenes ,Rats, Wistar ,Atrial Natriuretic Factor ,Plasmids - Abstract
A technique based on release of the human atrial natriuretic peptide (hANP) from plasmid hANP cDNA transfected Chinese hamster ovary (CHO) cells encapsulated in polycaprolactone (PCL)-capsules was used for a potential therapeutic approach to hypertension or congestive heart failure (CHF). The plasmid combining with hANP cDNA was transfected into CHO cells, and then encapsulated plasmid hANP cDNA transfected CHO cells were implanted into two-kidney, one-clip (2K1C) hypertensive rats intraperitoneally. The morphological changes, histological changes were investigated after the implantation of PCL-capsules in 2K1C hypertensive rats. The results showed that the implantation of encapsulated hANP-producing cells caused a significant delay of blood pressure (BP) increase after the encapsulated cells being implanted in 2K1C hypertensive rats. These effects were reflected morphologically by an attenuation of the glomerular sclerotic lesions, tubular damage and renal arterial thickening, comparing with control group. The plasma levels of hANP in 2K1C rats implanted with the PCL-capsules containing hANP-producing cells were higher than that of the control rats. These results demonstrated the usefulness of encapsulated hANP gene transfected cells as a new tool for hANP gene delivery in studying renovascular hypertension and cardiovascular diseases, thus implying the potential of using gene transfected cells as therapeutic agents in the treatment of cardiovascular diseases.
- Published
- 2004
224. RESEARCH ON THE PHYSICAL SIMULATION OF AUTO MOTORCYCLE BASED ON THE FINITE ELEMENT TECHNIQUE.
- Author
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WENGANG ZHOU and HUILING GUO
- Subjects
- *
MOTORCYCLES , *VIBRATION measurements , *FINITE element method , *MODAL analysis , *MOTORCYCLING - Abstract
In this paper, the authors aim at researching the physical simulation of auto motorcycle, based on the finite element technique. The performance of the motorcycle and its riding comfortableness are the crux of the matter. Thus, to solve the vibration problem has become urgent problematic issue, which puzzles our motorcycle manufacture enterprise for a long time. In this paper, the Finite Element Analyse (FEA) has been used to analyse the characteristics of the vibration of the frame. The dynamic characteristic has been deeply and systematically comprehended through the Modal Analysis and Harmonic Response Analysis. It provides the important basis of solving the vibration problem of the motorcycle and has some theoretical significance and important practical value. [ABSTRACT FROM AUTHOR]
- Published
- 2016
225. Re-sensitization of radiation resistant colorectal cancer cells to radiation through inhibition of AMPK pathway.
- Author
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HONGYONG JIN, SUJIE GAO, HUILING GUO, SHENGNAN REN, FUJIAN JI, ZHUO LIU, and XUEBO CHEN
- Subjects
RADIOTHERAPY ,COLON cancer treatment ,CANCER cells ,PHYSIOLOGICAL effects of adenylic acid ,CANCER relapse ,PREVENTION - Abstract
Radiotherapy (RT) is commonly used to treat multi-tumors to attenuate the risk of recurrence. Despite impressive initial clinical responses, a large proportion of patients experience resistance to RT. Therefore, identification of functionally relevant biomarkers would be beneficial for radioresistant patients. Adenosine monophosphate-activated kinase (AMPK) is recognized as a mediator of tumor suppressor gene function. In the present study, radio-sensitive and -resistant colon cancer patient samples were compared and the AMPK pathway was observed to be highly activated in radioresistant patients. In addition, the protein and mRNA levels of AMPK were upregulated in radioresistant colon cancer cells in comparison to radiosensitive colon cancer cells. The present study provides evidence that activation of AMPK by metformin contributes to radioresistance. Inhibition of AMPK by either small interfering RNA or Compound C, which is a specific inhibitor of AMPK, re-sensitized radiation resistant cells. The data presented indicates a synergistic effect on radiation resistant cancer cells by the combination of Compound C and radiation. In summary, the present study proposes that inhibition of the AMPK pathway is a potential strategy for reversing radiation resistance and may contribute to the development of therapeutic anticancer drugs. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
226. A Novel Method for Detecting Similar Microblog Pages based on Longest Common Subsequence.
- Author
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Wengang Zhou, Huiling Guo, and Yang Zhao
- Subjects
MICROBLOGS ,INFORMATION sharing - Abstract
Microblog is a relation-based platform for sharing, spreading and acquiring information and it is also the source of internet public opinion and the important battlefield of information transmission. The convenient forwarding operations of microblog result in the rapid spread of plenty of identical or similar microblog pages in the microblog space. Therefore, the detection of similar microblog pages is of great importance to lighten the client's burden of browsing and improve the analytic efficiency of internet public opinion. A method based on LCS(Longest Common Subsequence) is introduced to detect similar microblog page: First is to calculate the files' subset of the possibly similar microblog pages, and next is to calculate its LCS and extract the reliable parts so as to ultimately detect the similar microblog pages. Experiments show that this method can detect the similar pages from the microblog data accurately and efficiently. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
227. An Image Matching Algorithm using Thin Plate Splines (TPS) Transformation Model.
- Author
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Huiling Guo, Yan Hou, and Yang Zhao
- Subjects
IMAGE registration ,SPLINES ,MATHEMATICAL functions - Abstract
In this paper, through the analysis of the registration of edge feature points and the principle of distribution, the research on the image stitching and recovery is conducted by selecting regular images. Through the research, we can find that the image registration of edge feature points concludes two important steps: one is the criterion of feature matching; the other is the image edge feature points to be extracted. Through the estimation of the TPS transformation parameters between the stitching images, the final accurate TPS transformation parameters and the matching relation are obtained through the mutual approximation of the feature points set between the parameters. The validity of this study is verified by the example. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
228. Long-term outcomes and complications of Moscow Eye Microsurgery Complex in Russia (MICOF) keratoprosthesis following ocular surface burns: clinical experience in China.
- Author
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Liqiang Wang, Yifei Huang, Gaiping Du, Ying Dong, Huiling Guo, Dajiang Wang, Jifeng Yu, Qun Wang, Bing Chen, and Lulu Hou
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MICROSURGERY ,HEALTH outcome assessment ,SURGICAL complications ,ARTIFICIAL corneas ,EYE burns ,THERAPEUTICS - Abstract
Objective The aim of this study was to evaluate the clinical outcomes of the implantation of MICOF keratoprosthesis (Kpro) in patients with alkaline, acid and thermal ocular burns. Methods This is a retrospective, non-competitive, interventional case series. Ninety eyes of 90 patients with ocular burns underwent MICOF KPro surgery at the Chinese People's Liberation Army General Hospital between April 2000 and December 2012. Preoperative and postoperative findings were recorded. Retention of the prostheses and the treatment for postoperative complications were investigated. Results The mean age of patients was 40.26 ±12.18 years (range: 8-64 years), and the mean duration after ocular trauma was 4.8±6.2 years (range: 1.5-12 years). The mean follow-up period was 58.22 ±36.28 months (range: 1-145 months). Eighty patients were followed for ≥1 year and 73 eyes (81.11%) achieved the best-corrected visual acuity of 20/200 or better and 39 eyes (43.33%) achieved best-corrected visual acuity of ≥20/40. The common complications were glaucoma, corneal melt and conjunctiva overgrowth, and the incidences of these complications were 59.99%, 40% and 31.11%, respectively. One patient experienced KPro extrusion, and two patients exhibited leakage from the area of the implant. Seven with endophthalmitis eyes had final visual acuities of light perception. There was no significant difference in the effectiveness of the implants among the different causes of injuries, including acid, alkali and thermal burns. Conclusions MICOF Kpro is an effective alternative for patients with ocular burn with corneal blindness. Glaucoma and endophthalmitis were identified as significant risk factors for visual loss. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
- View/download PDF
229. Erratum: Axin determines cell fate by controlling the p53 activation threshold after DNA damage
- Author
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Qinxi Li, Shuyong Lin, Xuan Wang, Guili Lian, Zailian Lu, Huiling Guo, Ka Ruan, Yanhai Wang, Zhiyun Ye, Jiahuai Han, and Sheng-Cai Lin
- Subjects
Cell Biology - Published
- 2009
230. A license plate recognition algorithm based on image processing technology.
- Author
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Huiling Guo, Wengang Zhou, and Yang Zhao
- Subjects
- *
IMAGE processing , *INTELLIGENT transportation systems , *GENETIC algorithms - Abstract
With the development of society, license plate recognition systems had become an important part of intelligent transportation systems, and had been widely used. In this paper, the existing license plate recognition algorithm was optimized by the image processing technology. Firstly, by means of the collected original gray-scale image processing, three methods of color-to-gray image were proposed. Then the edge detection was carried out, and the Sobel operator was chose as the optimal edge detection method by using four kinds of edge detection operators. Finally, according to the genetic algorithm construct the method of boundary location, the license plate was located on the basis of the column scan, and accuracy rate was up to 97%. [ABSTRACT FROM AUTHOR]
- Published
- 2015
231. Downregulation of Clock in circulatory system leads to an enhancement of fibrinolysis in mice.
- Author
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Shuting Cheng, Zhou Jiang, Yan Zou, Chen Chen, Yuhui Wang, Yanyou Liu, Jing Xiao, Huiling Guo, and Zhengrong Wang
- Published
- 2011
- Full Text
- View/download PDF
232. A meta Analysis of Gemcitabine plus Platinum Chemotherapy Compared with Single-agent Chemotherapy in the Treatment of Non-small Cell Lung Cancer.
- Author
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Yunjiu GOU, Lingjuan ZHANG, Qimei YANG, Rongfang ZHANG, Huiling GUO, Lei JIANG, Kehu YANG, and Jinhui TIAN
- Subjects
META-analysis ,LUNG cancer ,CANCER treatment ,CANCER chemotherapy ,COMPARATIVE studies ,THROMBOCYTOPENIA ,BLOOD platelet disorders ,CISPLATIN - Abstract
Background and objective Whether gemcitabine plus platinum chemotherapy is superior to gemcitabine or platinum single-agent chemotherapy for patients with non-small cell lung cancer (NSCLC) is still in dispute, and the aim of this study is to evaluate the efficacy and safety of gemcitabine combining platinum chemotherapy for patients with NSCLC. Methods We searched relevant randomized controlled trials (RCTs) from VIP, CBM, CNKI, the Cochrane library, PUBMED and EMBASE. We traced the related references and experts in this field and communicated with other authors to obtain the information that has not been found. We made quality assessment of qualified RCTs assessed by the exclusion and inclusion criteria and used RevMan 5.0 provided by the Cochrane Collaboration to perform meta-analysis. Results Four RCTs were eligible and included 984 patients. Meta analysis results suggested that: compared with gecitabine single-agent chemotherapy, the combination had a statistically significant benefit in increasing the response rate (OR=3.29, 95%CI: 1.79-6.05, P=0.000 1) and 2-year survival rate (OR=3.22, 95%CI: 1.45-7.12, P=0.004) while increased the risk of the incidence of adverse reactions, especially the grade 3-4 thrombocytopenia (RR=8.16, 95%CI: 1.71-39.07, P=0.009); compared with cisplatin single-agent chemotherapy, the combination had a statistically significant benefit in increasing the response rate (OR=3.51, 95%CI: 2.20-5.60, P<0.01) and 1-year survival rate (OR=1.67, 95%CI: 1.16-2.41, P=0.006) while increased the risk of the incidence of adverse reactions, especially the grade 3-4 thrombocytopenia (OR=28.55, 95%CI: 14.06-57.04, P<0.01). Conclusion Compared with single-agent chemotherapy, the combining can significantly improve the efficiency and survival rate while increase the toxicity rare. The results still need to be proved by high quality RCTs. [ABSTRACT FROM AUTHOR]
- Published
- 2010
- Full Text
- View/download PDF
233. A Systematic Review of Erlotinib for Advanced Non-small Cell Lung Cancer.
- Author
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Huiling Guo, Rongfang Zhang, Qimei Yang, Lingjuan Zhang, Kehu Yang, and Jinhui Tian
- Subjects
THERAPEUTICS research ,TUMORS ,CANCER patients ,LUNG cancer treatment ,SYSTEMATIC reviews ,DRUG efficacy ,CANCER cells ,SURVIVAL analysis (Biometry) ,RESPONSE rates - Abstract
Background and objective It was unclear whether advanced non-small cell lung cancer (NSCLC) patients could benefit from erlotinib therapy. This study was aimed to evaluate the efficacy and safety of NSCLC patients treated with erlotinib. Methods The relevant randomized controlled trials (RCT) were searched from VIP, CMB, CNKI, PubMed, EMBASE and The Cochrane Library. The related references and experts in this field were traced, and other authors were communicated with to obtain the information that has not been found. Quality assessment of qualified RCTs assessed by the exclusion and inclusion criteria and RevMan 5.0 provided by the Cochrane Collaboration was used to perform meta-analysis. Results Three RCTs involving 2 969 patients were included. Meta analysis results suggested that erlotinib was superior to placebo for one year survival rate (OR=1.18, 95%CI: 1.01-1.38), tumor response rate (OR=1.24, 95%CI: 1.03-1.49), median overall survival, median progression-free survival and tumor responses duration. At the same time, the incidence of grade 3-4 skin rash (OR=16.33, 95%CI: 7.01-38.02) and diarrhea (OR=5.02, 95%CI: 2.93-8.60) of the adverse reactions was increased. Conclusion Advanced non-small cell lung cancer patients could benefit from Erlotinib therapy, but the incidence of skin rash and diarrhea was significantly increased, and in the absence of damage to the blood system, serious liver and kidney damage, cardiac toxicity, etc, there were no difference with placebo. [ABSTRACT FROM AUTHOR]
- Published
- 2009
- Full Text
- View/download PDF
234. Axin determines cell fate by controlling the p53 activation threshold after DNA damage.
- Author
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Qinxi Li, Shuyong Lin, Xuan Wang, Guili Lian, Zailian Lu, Huiling Guo, Ka Ruan, Yanhai Wang, Zhiyun Ye, Jiahuai Han, and Sheng-Cai Lin
- Subjects
DNA damage ,P53 antioncogene ,APOPTOSIS ,CELL death ,GENETIC toxicology ,CELL cycle - Abstract
Cells can undergo either cell-cycle arrest or apoptosis after genotoxic stress, based on p53 activity. Here we show that cellular fate commitment depends on Axin forming distinct complexes with Pirh2, Tip60, HIPK2 and p53. In cells treated with sublethal doses of ultra-violet (UV) radiation or doxorubicin (Dox), Pirh2 abrogates Axin-induced p53 phosphorylation at Ser 46 catalysed by HIPK2, by competing with HIPK2 for binding to Axin. However, on lethal treatment, Tip60 interacts with Axin and abrogates Pirh2–Axin binding, forming an Axin–Tip60–HIPK2–p53 complex that allows maximal p53 activation to trigger apoptosis. We also provide evidence that the ATM/ATR pathway mediates the Axin–Tip60 complex assembly. An axin mutation promotes carcinogenesis in Axin
Fu /+ (Axin-Fused) mice, consistent with a dominant-negative role for AxinFu in p53 activation. Thus, Axin is a critical determinant in p53-dependent tumour suppression in which Pirh2 and Tip60 have different roles in triggering cell-cycle arrest or apoptosis depending on the severity of genotoxic stress. [ABSTRACT FROM AUTHOR]- Published
- 2009
- Full Text
- View/download PDF
235. Remote neurostimulation through an endogenous ion channel using a near-infrared light-activatable nanoagonist.
- Author
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Weifeng Tian, Qi Jia, Jiewen Lin, Jiamin Luo, Dongmei He, Jie Yang, Tao Guo, Huiling Guo, Yusheng Guo, Wenjie Zhang, Feiyu Chen, Ying Ye, Jingjing Liu, Mindong Xu, Chengjie Deng, Boxiang Cui, Deyuan Su, Hao Wang, Yi Lu, and Jianru Xiao
- Subjects
- *
ION channels , *TRP channels , *NEURAL stimulation , *FLAVIN adenine dinucleotide , *INTRATHECAL injections , *SENSORY neurons - Abstract
The development of noninvasive approaches to precisely control neural activity in mammals is highly desirable. Here, we used the ion channel transient receptor potential ankyrin-repeat 1 (TRPA1) as a proof of principle, demonstrating remote near-infrared (NIR) activation of endogenous neuronal channels in mice through an engineered nanoagonist. This achievement enables specific neurostimulation in nongenetically modified mice. Initially, target-based screening identified flavins as photopharmacological agonists, allowing for the photoactivation of TRPA1 in sensory neurons upon ultraviolet A/blue light illumination. Subsequently, upconversion nanoparticles (UCNPs) were customized with an emission spectrum aligned to flavin absorption and conjugated with flavin adenine dinucleotide, creating a nanoagonist capable of NIR activation of TRPA1. Following the intrathecal injection of the nanoagonist, noninvasive NIR stimulation allows precise bidirectional control of nociception in mice through remote activation of spinal TRPA1. This study demonstrates a noninvasive NIR neurostimulation method with the potential for adaptation to various endogenous ion channels and neural processes by combining photochemical toolboxes with customized UCNPs. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
236. Performance analysis for selection combining in Rician environments.
- Author
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Fang Zhang, Wei Chen, Huiling Guo, and Xuefei Liu
- Published
- 2011
- Full Text
- View/download PDF
237. Shaking Hands but Not Sharing Gains: How Relationships Facilitate Dynamic Marketing Capabilities Development in an Emerging Economy.
- Author
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Hangjun Xu, Huiling Guo, Jing Zhang, and Anh Dang
- Subjects
MARKETING strategy ,ENTREPRENEURSHIP ,MARKET orientation ,INNOVATIONS in business ,ORGANIZATIONAL performance - Published
- 2017
238. Part I: Marketing Strategy and Management: Linking Marketing Capabilities to Firm Performance: Differential Effects of Three Types of Marketing Capabilities on Firm Performance: The Key Role of Adaptive Capability in Environmental Turbulence.
- Author
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Huiling Guo, Hangjun Xu, Chuanyi Tang, and Zhaoyang Guo
- Subjects
MARKETING ,TURBULENCE ,ORGANIZATIONAL performance - Abstract
An abstract of the article "Differential Effects of Three Types of Marketing Capabilities on Firm Performance: The Key Role of Adaptive Capability in Environmental Turbulence" by authors including Huiling Guo, Zhaoyang Guo, and Chuanyi Tang is presented.
- Published
- 2015
239. Selective binding and magnetic separation of His-tagged proteins using Fe3O4/PAM/NTA-Ni2+ Magnetic Nanoparticles.
- Author
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Huiling Guo, Mengyun Li, Shu Tu, and Honghao Sun
- Published
- 2018
- Full Text
- View/download PDF
240. The role of ERK1/2 in colitis through regulation of NADPH oxidase and mitochondrial fission
- Author
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Zhai Xiaoming, Tan Siwei, Yi Yujun, Liu Huiling, Guo Jiaxiang, Wu Shuyun, Tao Jin
- Subjects
ulcerative colitis ,extracellular signal regulated kinase 1/2 ,nadph oxidase ,mitochondrial fission ,Medicine - Abstract
Objective To investigate the role of extracellular signal regulated kinase 1/2 (ERK1/2) in colitis through the regulation of NADPH oxidase and mitochondrial fission. Methods Mice models of acute colitis were induced by 3% dextran sulfate sodium (DSS). Thirty C57BL/6J mice were randomly divided into six groups by random number table method: control group, 3%DSS group,1% dimethyl sulfoxide (DMSO) group, ERK1/2 inhibitor(PD98059) group, 3%DSS+1%DMSO group and 3%DSS+PD98059 group, with five mice in each group. The changes of body weight, colonic length, disease activity index and colonic histopathological changes of mice in the control and 3%DSS groups were evaluated, and the expression levels of ERK1/2, p-ERK1/2, Nicotinamide adenine dinucleotide phosphate oxidase 1 (Nox1) and Nox2 in colonic mucosa of mice were detected. Mice in 1%DMSO and 3%DSS+1%DMSO groups were intraperitoneally injected with 1%DMSO. Mice in the PD98059 and 3%DSS+PD98059 groups were intraperitoneally injected with PD98059. The colonic histopathological changes were evaluated among four groups, and the expression levels of Nox1, Nox2, Dynamin related protein 1 (DRP1), p-DRP1-S616 and p-DRP1-S637 mitochondrial fission related proteins were detected. Mitochondrial fission of colonic epithelial cells in the control and 3%DSS groups was observed by transmission electron microscopy. The co-localization of Nox2 and mitochondrial outer membrane translocator enzyme TOM complex (TOMM20) in colonic mucosa of mice in two groups was analyzed by double-immunofluorescence staining. The correlation between relative expression levels of DRP1 and Nox2 mRNA in mouse colonic mucosa was analyzed in two groups. Results Compared with the control group, mice in the 3%DSS group exhibited body weight loss, shortened colonic length, increased disease activity index and increased colonic histopathological score. The expression levels of p-ERK1/2, Nox1, Nox2 in colonic mucosa of mice were significantly up-regulated in the 3%DSS group (all P < 0.05). In mice with colitis, mitochondrial fission in colonic epithelial cells was increased, and the colonic mucosa co-localization of DRP1 and Nox2 was elevated, and the relative mRNA expression levels of both target genes were positively correlated (r = 0.678, P < 0.05). ERK1/2 inhibitor PD98059 improved colonic histopathological changes in mice with colitis, and down-regulated the expression levels of Nox1, Nox2, DRP1, p-DRP1-S616 in colonic mucosa. Conclusion Inhibition of ERK1/2 may ameliorate colitis by down-regulating NADPH oxidase expression and alleviating mitochondrial fission.
- Published
- 2023
- Full Text
- View/download PDF
241. Influence of culture conditions and preconditioning on survival of Lactobacillus delbrueckii subspecies bulgaricus ND02 during lyophilization.
- Author
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Yuyu Shao, Shuran Gao, Huiling Guo, and Heping Zhang
- Subjects
- *
LACTOBACILLUS delbrueckii , *FREEZE-drying , *HEAT shock proteins , *GENE expression in bacteria , *POLYMERASE chain reaction - Abstract
The cryotolerance of Lactobacillus delbrueckii ssp. bulgaricus is weak during vacuum freeze-drying. Many factors affect cryoresistance of these bacteria, such as cryoprotectant composition, the lyophilization technology used, and the intrinsic characteristics of the bacteria. In this research, we explored the fermentation technology and other preconditioning treatments of cells in improving the cryoresistance of Lactobacillus delbrueckii ssp. bulgaricus strains during lyophilization. The addition of yeast extract in the propagation medium exerted a negative effect on the cryotolerance of these bacteria and decreased survival during lyophilization. The count of the freeze-dried cells from medium containing a high level (4%) of yeast extract was only 4.1 x 109 cfu/g, indicating a death rate as high as 88%, compared with the culture medium without yeast extract, with a lower death rate of 44.7%. When Lactobacillus delbrueckii ssp. bulgaricus ND02 was propagated in yeast extract-free de Man, Rogosa, and Sharpe broth at a set pH value of 5.1, the cells showed unexpectedly higher survival after freeze-drying. Viable counts of the lyophilized cell of strain ND02 cultivated at pH 5.1 could reach 1.05 x 1011 cfu/g and survival of the freeze-drying process was 68.3%, whereas at pH 5.7, survival was only 51.2%. We also examined the effects of pretreatment of cells on survival of the bacteria after vacuum freeze-drying. By analyzing the effect of pretreatment conditions on the expression of cold- and heat-shock genes, we established 2 pretreatments that improved survival of cells after lyophilization. Optimal fermentation conditions and pre-treatment of the cell-cryoprotectant mixture at 10°C for 2 h or 37°C for 30 min improved the cryoresistance of 4 strains of Lactobacillus delbrueckii ssp. bulgaricus to varying degrees. Cells of IMAU20269 and IMAU20291 that were pretreated showed enhanced survival of 16.06 and 16.82%, respectively, after lyophilization. Expression of cold- and heat-shock genes for pretreated strains ND02, IMAU80423, IMAU20269, and IMAU20291 was analyzed by using quantitative PCR. From the expression of 2 cold shock-induced genes (cspA and cspB) and 6 heat shock-induced genes (groES, hsp, hsp20, hsp40, hsp60, and hsp70), strain ND02 showed a higher relative quantity of gene expression and displayed superior resistance to cold-induced stress during the freeze-drying process. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
242. HLA—A Typing in Human Serum and Salive
- Author
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LU HUILING GUO JINGYUAN(DEPARTMENT OF FORENSIC MEDICINE;ZHONGSHAN MEDICAL COLLEGE)
- Subjects
Medicine - Abstract
The soluble HLA antigens in human serum and saliva were determined with microlymphocytoxicity inhibition test (MLIT).As compared with the results of positive and negative salive determined, the difference was very significant(P
- Published
- 1985
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