248 results on '"Huang, Dong-Sheng"'
Search Results
202. Trimetazidine in conditions other than coronary disease, old drug, new tricks?
- Author
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Zou H, Zhu XX, Ding YH, Jin QY, Qian LY, Huang DS, and Cen XJ
- Subjects
- Acetyl-CoA C-Acyltransferase metabolism, Chemically-Induced Disorders etiology, Drug Repositioning, Humans, Mitochondria, Heart metabolism, Vasodilator Agents pharmacology, Antineoplastic Agents adverse effects, Chemically-Induced Disorders drug therapy, Coronary Artery Disease drug therapy, Coronary Artery Disease metabolism, Diabetic Cardiomyopathies drug therapy, Kidney Diseases chemically induced, Kidney Diseases drug therapy, Trimetazidine pharmacology
- Abstract
Trimetazidine (TMZ) has traditionally been used as an anti-ischemic drug for coronary artery disease by selectively inhibiting the mitochondrial long-chain 3-ketoacyl-CoA thiolase. Recently, new applications for this therapy have been investigated. This article reviews alternative uses for TMZ in non-coronary artery diseases, such as non-ischemic cardiomyopathy, sepsis, myocardial dysfunction induced by anti-cancer drugs, diabetic cardiomyopathy and contrast-induced nephropathy., (Copyright © 2017. Published by Elsevier B.V.)
- Published
- 2017
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203. [Prognostic Significance of Joint Detection of miR-210 and Minimal Residual Disease in Pediatric Acute Lymphoblastic Leukemia].
- Author
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Mei YY, Li ZG, Zhang Y, Zhang WL, Zhang PW, Wang N, and Huang DS
- Subjects
- Child, Disease-Free Survival, Humans, Precursor Cell Lymphoblastic Leukemia-Lymphoma metabolism, Prognosis, Recurrence, Treatment Outcome, MicroRNAs metabolism, Neoplasm, Residual, Precursor Cell Lymphoblastic Leukemia-Lymphoma drug therapy
- Abstract
Objective: To detect the expression of microRNA-210(miR-210) in childhood acute lymphoblastic leukemia(ALL), and to evaluate the role of the joint detection of miR-210 and MRD in the prognosis and clinical treatment of pediatric ALL., Methods: Eighty-eight children diagnosed with ALL were included in the study. miR-210 was quantitatively detected by real-time quantitative polymerase chain reaction(RQ-PCR) in 88 ALL patients. The average Ct value of samples obtained from 5 pediatric ALL patients with long-term complete continuous remission (CCR>5 years) was used as a calibrator. The expression levels of miR-210 in newly diagnosed patients was calculated by the 2
-ΔΔCt method and presented as multiple changes compared with that of the 5 CCR patients., Results: The expression of miR-210 in the favorable prognosis group was significantly higher than that in the unfavorable prognosis group (10.64±1.5 vs 3.27±0.68)(P<0.05). Compared with the miR-210 high-expression group, poorer relapse-free survival(RFS), event-free survival(EFS) and overall survival(OS) (P all <0.001) were found in the low-expression group. Based on the expression of miR-210 and MRD, the 88 cases were divided into 3 groups. The relapse rate of miR-210-MRD high-risk group (70%) was significantly higher than that of the miR-210-MRD middle-risk group(6.25%) and miR-210-MRD low-risk group (2.1%). Kaplan-Meier survival analysis demonstrated that the miR-210-MRD high-risk group had poorer RFS, EFS and OS than those in other 2 groups (P all <0.01)., Conclusion: The expression level of miR-210 is an independent prognostic factor for pediatric ALL, and the miR-210 is a good useful indicator for predicting the relapse and induction failure of childhood ALL. Joint detection of miR-210 and MRD can help predict outcomes more precisely, thus may be used as an effective mean of determining prognosis, monitoring recurrence, and guiding treatment.- Published
- 2017
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204. [Clinical features and outcomes of neuroblastoma patients aged above 5 years].
- Author
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Zhang Y, Zhang WL, Huang DS, Han T, Zhi T, Li J, Yi Y, Wen Y, Li F, Mei YY, and DU YY
- Subjects
- Adolescent, Child, Child, Preschool, Combined Modality Therapy, Female, Humans, Male, Neuroblastoma mortality, Retrospective Studies, Neuroblastoma therapy
- Abstract
Objective: To investigate the clinical features and outcomes of neuroblastoma (NB) children aged above 5 years, and to provide a theoretical basis for improving prognosis., Methods: A retrospective analysis was performed for the clinical data of 54 previously untreated NB children, and their clinical features and outcome were analyzed. The Kaplan-Meier method was used for survival analysis., Results: Among the 54 children, there were 36 boys and 18 girls, and all of them had stage 3 or 4 NB. Of all the children, 41 (41/54, 76%) had retroperitoneal space-occupying lesions, 10 (10/54, 18%) had mediastinal space-occupying lesions, 2 (2/54, 4%) had intraspinal space-occupying lesions, and 1 (1/54, 2%) had pelvic space-occupying lesions. At the end of the follow-up, 30 children (30/54, 56%) survived, among whom 23 (77%) achieved disease-free survival (9 achieved complete remission after chemotherapy for recurrence), 6 (20%) achieved partial remission of tumor (all of them received chemotherapy again due to recurrence), and 1 (3%) experienced progression (with progression after chemotherapy again due to recurrence); 24 children (44%) died, among whom 22 died after chemotherapy again due to recurrence and 2 died of multiple organ failure during the first treatment. According to the Kaplan-Meier survival analysis, the mean survival time was 53.8 months, and the children with stage 3 NB had a significantly higher overall survival rate than those with stage 4 NB (80% vs 53%; p<0.01). The children with recurrence had a significantly lower mean survival time than those without recurrence (51.68 months vs 62.57 months; p<0.01)., Conclusions: Older children often have late-stage NB, but standard treatment can improve their outcomes.
- Published
- 2016
205. High-dose chemotherapy combined with autologous peripheral blood stem cell transplantation in children with advanced malignant solid tumors: A retrospective analysis of 38 cases.
- Author
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Zhang WL, Zhang YI, Zhi T, Huang DS, Wang YZ, Hong L, Zhu X, Liu AP, and Hu HM
- Abstract
The aim of the present study was to assess the toxicity and efficacy of autologous peripheral blood stem cell (APBSC) transplantation in children with advanced malignant solid tumors. The outcomes of 38 children with advanced malignant solid tumor, who were treated with high-dose chemotherapy and autologous peripheral blood stem cell transplantation in Beijing Tongren Hospital (Capital Medical University, Beijing, China) between September 2005 and November 2011, were retrospectively analyzed. The effects of treatment were evaluated according to the standard Bearman's criteria. The mean count of collected mononuclear cells and the cluster of differentiation 34
+ cell count from 38 patients was 5.6±2.2×108 /kg and 3.8±2.6×106 /kg, respectively. From these 38 patients, the number of stem cells collected from 31 cases (81.6%) accorded with the transplantation standards. Three and 14 days after pretreatment in these 38 cases, there were 19 cases of grade I, 11 cases of grade II, five cases of grade III and three cases of grade IV (one case succumbed) adverse reaction. Following the treatment (23-40 days after pretreatment, during organ injury recovery), 37 cases obtained bone marrow reconstitution with a mean time of 12.3±3.1 days after APBSC reinfusion. The median survival time of the 37 patients was 49 months, and the survival rate at one, three and five years post-treatment was 91.9, 68.2 and 36.6%, respectively.- Published
- 2015
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206. [Treatment of advanced malignant solid tumor by autologous peripheral blood stem cell transplantation in children: experience of 38 cases].
- Author
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Zhang Y, Zhang WL, Huang DS, Wang YZ, Hong L, Zhu X, Liu AP, Zhi T, and Hu HM
- Subjects
- Adolescent, Child, Child, Preschool, Female, Hematopoiesis, Humans, Infant, Leukocyte Count, Male, Neoplasms blood, Neoplasms mortality, Transplantation, Autologous, Neoplasms surgery, Peripheral Blood Stem Cell Transplantation
- Published
- 2014
207. rna interference targeting p110β reduces tumor necrosis factor-alpha production in cellular response to wear particles in vitro and osteolysis in vivo.
- Author
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Huang JB, Ding Y, Huang DS, Zeng WK, Guan ZP, and Zhang ML
- Subjects
- Animals, Cell Line, Ceramics adverse effects, Class I Phosphatidylinositol 3-Kinases biosynthesis, Class I Phosphatidylinositol 3-Kinases metabolism, Enzyme Activation, Inflammation immunology, Joint Prosthesis adverse effects, Lentivirus genetics, Macrophages, Male, Mice, Mice, Inbred C57BL, Nanoparticles adverse effects, Osteoclasts metabolism, Proto-Oncogene Proteins c-akt metabolism, RNA Interference, RNA, Messenger biosynthesis, RNA, Small Interfering, Titanium adverse effects, Tumor Necrosis Factor-alpha biosynthesis, Tumor Necrosis Factor-alpha genetics, Arthroplasty, Replacement adverse effects, Class I Phosphatidylinositol 3-Kinases genetics, Osteolysis metabolism, Prosthesis Failure adverse effects, Tumor Necrosis Factor-alpha metabolism
- Abstract
Aseptic joint loosening is a key factor that reduces the life span of arthroplasty. There are currently few effective treatments for joint loosening except surgical revision. We explored the inhibitory effects of p110β-targeted small interfering RNA (siRNA) and lentivirus on particle-induced inflammatory cytokine expression in the murine macrophage cell line, RAW264.7. siRNA- and lentivirus-targeting p110β were transfected and infected prior to particle stimulation, respectively. Ceramic and titanium particles of different sizes were prepared to stimulate macrophages. Fluorescence microscopy showed that the efficiency of siRNA transfection and lentivirus infection were 74.2 ± 4.2 and 92.3 ± 2.6 %, respectively. TNF-alpha mRNA in the particle stimulation plus RNA interference (RNAi) groups were significantly lower compared with the particle stimulation-only groups (P < 0.05). Similarly, protein levels of TNF-alpha in RNAi-treated groups were significantly decreased after transfection or infection (P < 0.05). It showed that Phosphor-AKT (Ser473) activation was significantly reduced by RNAi through western blot. As assessed by CT, micro-CT and histological analysis, particle implantation induced a significant osteolysis in mice calvaria, which was limited by p110β lentivirus addition. These results suggested that p110β subtype of PI3K, followed by activation of Ser473, may possibly participate in the regulation of macrophages activity by wear particles, ultimately resulting in the TNF-α secretion and osteolysis.
- Published
- 2013
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208. [The clinical characteristics and prognosis of 92 cases of delayed occurrence of retinoblastoma].
- Author
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Zhang Y, Zhang WL, Wang YZ, Shi JT, Ma JM, Huang DS, Hu HM, Hong L, Liu AP, and Li J
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- Adolescent, Child, Child, Preschool, Female, Humans, Male, Prognosis, Retrospective Studies, Retinal Neoplasms diagnosis, Retinoblastoma diagnosis
- Abstract
Objective: To retrospectively summarize the clinical features, diagnosis and treatment of retinoblastoma (RB) patients in the children aged 5 years or above., Methods: This was a case-series study. Total of 92 patients (107 eyes) with RB aged 5 years or above in Department of Pediatric,Beijing Tongren Hospital from September 2005 to May 2010 were included in the study. The diagnosis of RB was established using clinical examination and histopathology analysis.Statistical analysis in the characteristics of disease and initial symptoms was performed in 92 cases. Kaplan-Meier curve was employed to analyze the survival function., Results: (1) The medium age of the patients was 6.25-year. Male accounted for 66.30% (61/92) and female was 33.69% (31/92) . According to the results of statistical scatter diagram, the age of the disease onset was ranged from 5.00 to 8.33 years. Percentile for age 5.77, 5.83 and 7.85 years were the 25th, 50th, 75th respectively. (2) In 92 cases, 83.69% (77/92) of the patients were unilateral RB and 16.31% (15/92) were bilateral RB. (3) Leukocoria was the most common manifestation in the diagnosis early RB, accounting for 55.43% (51/92) .Secondary symptoms were blurred vision, eye redness and photophobia, accounting for 23.91% (22/92). Other symptoms included strabismus, proptosis, glaucoma and the abnormal in fundus examination, accounting for 20.64% (19/92) .No family history of RB was found in all of cases studied. (4) Among 107 eyes with RB, 76 eyes were enucleated up to February 2013. The rate of enucleation was 71.02%. (5) By February 2013, the average following-up time was 39 months, during the time 1 case was lost, 6 cases were dead, the overall survival rate was 93.46% (85/92) . The result of Kaplan-Meier curve showed that the estimates of 5-year expected average total survival time was (72.61 ± 1.89) months(95%confidence interval was 68.9-76.3 months)., Conclusions: Unilateral RB are the most common cases in the age of 5 years or above, and the most common symptom is Leukocoria.Overall survival rate of older RB patient is high with comprehensive treatment.
- Published
- 2013
209. [The transhepatic approach in sophisticated hepatobiliary surgery].
- Author
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Hong DF, Huang DS, and Peng SY
- Subjects
- Humans, Biliary Tract Diseases surgery, Liver Diseases surgery
- Published
- 2013
210. Curcumin induces apoptosis of triple-negative breast cancer cells by inhibition of EGFR expression.
- Author
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Sun XD, Liu XE, and Huang DS
- Subjects
- Cell Line, Tumor, Cell Proliferation drug effects, Female, Humans, Mitogen-Activated Protein Kinase 1 metabolism, Mitogen-Activated Protein Kinase 3 metabolism, Phosphorylation, Receptors, Estrogen antagonists & inhibitors, Triple Negative Breast Neoplasms metabolism, Triple Negative Breast Neoplasms pathology, Apoptosis drug effects, Curcumin pharmacology, Receptors, Estrogen metabolism
- Abstract
Curcumin is the major component of the spice turmeric, extracted from the rhizomes of the plant Curcuma longa. It exerts a number of therapeutic effects, including the inhibition of cancer cell proliferation. However, the anti-carcinogenic mechanism of curcumin has not been fully elucidated. Triple-negative breast cancer (TNBC), which lacks expression of the estrogen receptor (ER), progesterone receptor (PR) and epidermal growth factor receptor 2 (HER2/EGFR2), is an aggressive breast cancer phenotype with a poor prognosis. In this study, we investigated the effects of curcumin on triple-negative breast cancer cells and the possible molecular mechanisms. The MDA-MB-231 TNBC cells were treated with curcumin, the growth inhibition ratio of the cells was measured by MTT assay, apoptosis was detected by flow cytometry and the expression levels of extracellular regulated protein kinase (ERK1/2), pERK1/2, EGFR and pEGFR were detected by western blotting. After treatment with different concentrations of curcumin, the growth inhibition rates of the MDA-MB-231 breast cancer cells of the 30 µmol/ml curcumin-treated group were significantly different from those of the other groups. The level of apoptosis of the curcumin-treated group (26.34%) was significantly different from that of the control group (2.76%). The expression levels of pERK1/2 and pEGFR in the curcumin-treated group were significantly decreased compared with those of the control group. These results indicate that curcumin is able to inhibit the proliferation of TNBC cells. Inhibition of the EGFR signaling pathway is the likely underlying molecular mechanism.
- Published
- 2012
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211. [Clinical features of 39 children with head and neck rhabdomysarcoma in a single medical center, and treatment outcomes].
- Author
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Zhang WL, Zhang Y, Huang DS, Guo F, and Han T
- Subjects
- Adolescent, Chemoradiotherapy, Child, Child, Preschool, Combined Modality Therapy, Female, Head and Neck Neoplasms mortality, Humans, Infant, Male, Peripheral Blood Stem Cell Transplantation, Rhabdomyosarcoma mortality, Treatment Outcome, Head and Neck Neoplasms therapy, Rhabdomyosarcoma therapy
- Abstract
Objective: To study the clinical features and treatment outcomes of head and neck rhabdomysarcoma (RMS) in children., Methods: The clinical data and results of follow-up visits for 39 children with head and neck RMS were retrospectively reviewed. The children (23 males and 16 females) with a median age of 6 years old (ranged 3 months to 14 years) were admitted to the Beijing Tongren Hospital between November, 2004 and November, 2010., Results: The 39 children mainly presented with exophthalmos and eyelid swelling (56%, 22/39), rhinostegnosis and nasal bleeding (28%, 11/39) and check mass (15%, 6/39). Common primary sites were the eyelid and orbit (56%, 22/39), followed by the nasopharynx and ethmoid antrum (28%, 11/39). Thirty-seven of the 39 patients showed a definite pathologic type and the embryo type was the most common (89%, 33/37). Follow-up visits were carried out for 35 children, with a median follow-up time of 38 months (10-80 months). Of the 35 children, 4 cases received surgery alone, 1 case received chemotherapy alone, 12 cases received surgery plus chemotherapy, 2 cases received surgery plus radiochemotherapy, 13 cases received surgery, chemotherapy and radiochemotherapy (8 cases received 125I particles implants), 2 cases received surgery, chemotherapy, radiochemotherapy and autologous peripheral blood stem cells transplantation (APBSCT), and 1 case received chemotherapy and APBSCT. Seven cases relapsed and 5 cases died of brain metastasis. The total survival rate was 86% (30/35), the complete remission rate was 66% (23/35), and the partial remission rate was 20%. In the 8 cases receiving 125I particles implants, 6 survived without tumor., Conclusions: Exophthalmos and eyelid swelling are the main presentations in children with head and neck RMS. Common primary sites of this disease are the eye and nasopharynx. The most common pathologic type is embryo type. Comprehensive treatment, including chemotherapy, surgery, 125I particles implants and APBSCT therapy, can improve outcome.
- Published
- 2012
212. In vitro comparison of the biological activity of alumina ceramic and titanium particles associated with aseptic loosening.
- Author
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Ding Y, Qin CQ, Fu YR, Xu J, and Huang DS
- Subjects
- Animals, Cell Line, Cytokines chemistry, Enzyme-Linked Immunosorbent Assay methods, Gene Expression Regulation, In Vitro Techniques, Inflammation, Joint Prosthesis, Macrophages cytology, Mice, Osteolysis, Particle Size, Polymerase Chain Reaction methods, Prosthesis Design, RANK Ligand chemistry, Tumor Necrosis Factor-alpha chemistry, Tumor Necrosis Factor-alpha metabolism, Aluminum Oxide chemistry, Biocompatible Materials chemistry, Titanium chemistry
- Abstract
Prosthetic wear particles are thought to play a central role in the initiation and development of periprosthetic osteolysis, leading to aseptic loosening of prostheses. This study aimed to compare the biological activity of ceramic and titanium particles that are associated with particle-induced, aseptic joint loosening. Different sizes of alumina-ceramic particles and titanium particles were prepared to stimulate murine macrophage cells RAW 264.7, of which the expressions of tumor necrosis factor alpha (TNF-alpha) and receptor activator of nuclear factor-κB ligand (RANKL) were measured by qPCR and ELISA at various time points. In the presence of all particles, the expression of TNF-alpha increased in a time-dependent manner, whereas the expression of RANKL showed no regular expression patterns. Notably, particles of smaller sizes provoked significantly higher levels of TNF-alpha and RANKL than those of larger sizes. Compared to the titanium particles, the ceramic particles provoked a significantly lower production of TNF-alpha. Thus, the bioactivities of titanium and alumina ceramic particles were inversely proportional to the sizes of the particles, and the expression of RANKL was not parallel to that of TNF-alpha. The successful outcome of ceramic-on-ceramic artificial joint prostheses may be attributed to the low biological activity of ceramic particles, as evidenced here.
- Published
- 2012
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213. [Clinical application of 125I particle implantation in children with rhabdomysarcoma of the head and neck].
- Author
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Zhang Y, Guo F, Zhang WL, Huang DS, Hong L, and Han T
- Subjects
- Adolescent, Child, Child, Preschool, Female, Humans, Iodine Radioisotopes adverse effects, Male, Tomography, X-Ray Computed, Brachytherapy adverse effects, Head and Neck Neoplasms radiotherapy, Iodine Radioisotopes therapeutic use, Rhabdomyosarcoma radiotherapy
- Abstract
Objective: To study the efficacy and safety of 125I particle implantation in the treatment of rhabdomysarcoma (RMS) of the head and neck in children., Methods: Eight children (four males and four females) with RMS of the head and neck received 125I particle implantation targeted to the primary lesion area. The primary lesions were noted in eyelids or eyes in two children, in the orbit in four children, and in the nasal cavity or nasal wing in two children. Treatment outcomes and side effects were observed., Results: The follow-up visits averaged 45±17 months (median 43 months) in the eight children receiving 125I particle implantation. Five children achieved a complete remission, 2 children achieved a complete remission and 1 child died. The total survival rate was 88% in the 8 children. Local pigmentation was observed in all eight children (100%). Nubecula (one case), eyeball pain (one case), serious blurred vision (one case), cornea ulcer and blindness (one case) and bleeding of the nasal cavity (one case) were also observed. Except for nubecula and blindness, these side effects were improved by symptomatic treatment., Conclusions: 125I particle implantation appears to be effective in the treatment of RMS of the head and neck in children. Most treatment-related side effects can be improved by symptomatic treatment.
- Published
- 2012
214. [Association of matrix metalloproteinase 9 polymorphisms with adolescent idiopathic scoliosis in Chinese Han female].
- Author
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Huang DS, Liang GY, and Su PQ
- Subjects
- Adolescent, Asian People genetics, Child, China, Female, Genetic Association Studies, Genotype, Humans, Phenotype, Genetic Predisposition to Disease genetics, Matrix Metalloproteinase 9 genetics, Polymorphism, Single Nucleotide genetics, Scoliosis genetics
- Abstract
Objective: To determine whether the matrix metalloproteinase 9 gene (MMP9) polymorphism is associated with the onset or progression of adolescent idiopathic scoliosis (AIS) in Chinese Han female., Methods: Three single nucleotide polymorphisms (SNPs) (rs17576, rs2250889, rs1805088) were genotyped through TaqMan-based real-time PCR assay in 190 AIS patients and 190 controls, all of whom were females from Chinese Han population with matched age. Analyses performed included Hardy Weinberg equilibrium test, Pearson chi-square test, Logistic regression analysis, linkage disequilibrium analysis and haplotype analysis. The mean maximum Cobb angles with different genotypes in case-only dataset were also compared., Results: All 3 SNPs have reached Hardy-Weinberg equilibrium in the controls. Genotype and allele frequencies of all SNPs were found similar between cases and controls by Pearson chi-square test and Logistic regression. Genotype-phenotype analysis showed that patients with CC genotype in rs2250889 featured larger maximum Cobb angles., Conclusion: MMP9 may not be a predisposition gene of AIS in Han female. However, homozygous mutation in rs2250889 can render scoliosis more severe, implying that MMP9 defect may result in deterioration of AIS.
- Published
- 2011
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215. Blockade of IGF-IR exerts anticancer effects in hepatocellular carcinoma.
- Author
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Yao WF, Liu JW, Sheng GL, and Huang DS
- Subjects
- Apoptosis, Caspase 3 metabolism, Cell Line, Tumor, Cell Movement, Cytochromes c metabolism, Down-Regulation, Extracellular Signal-Regulated MAP Kinases metabolism, Humans, Phosphorylation, Receptor, IGF Type 1 metabolism, Antineoplastic Agents therapeutic use, Carcinoma, Hepatocellular drug therapy, Liver Neoplasms drug therapy, Receptor, IGF Type 1 antagonists & inhibitors, Tyrphostins therapeutic use
- Abstract
Hepatocellular carcinoma (HCC) is one of the most common cancers worldwide, and is characterized by a high degree of malignancy and a low survival rate. Most HCCs express insulin-like growth factors and their receptors (IGF-IR), which mediate signaling, promote survival and invasion, and prevent apoptosis. Thus, they may be potential targets for the treatment of HCCs. In the present study, the potential therapeutic effect of Tyrphostin AG1024 in HCC was examined. After treatment with various concentrations of AG1024 (one selective inhibitor of IGF-IR), AG1024 not only dose-dependently inhibited the proliferation of HCC cells and induced apoptosis, but also markedly inhibited invasion ability. Expression of proteins detected by Western blot analysis revealed that AG1024 dose-dependently increased the expression of cytochrome C, while procaspase-3 and phospho-ERK were down-regulated. Thus, IGF-IR inhibition may be a promising novel approach to the treatment of HCC.
- Published
- 2011
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216. [Mobilization and collection of autologous peripheral blood stem cells by CIE or IEV protocol in children with malignant solid tumors].
- Author
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Zhang Y, Zhang WL, Huang DS, Yang YP, Liu XC, and Wu YP
- Subjects
- Antineoplastic Combined Chemotherapy Protocols administration & dosage, Child, Child, Preschool, Epirubicin administration & dosage, Etoposide administration & dosage, Female, Granulocyte Colony-Stimulating Factor pharmacology, Hematopoietic Stem Cell Mobilization adverse effects, Humans, Ifosfamide administration & dosage, Male, Recombinant Proteins, Transplantation, Autologous, Antineoplastic Combined Chemotherapy Protocols pharmacology, Hematopoietic Stem Cell Mobilization methods, Neuroblastoma therapy, Peripheral Blood Stem Cell Transplantation, Rhabdomyosarcoma therapy
- Abstract
Objective: Autologous peripheral blood stem cell transplantation (APBSCT) is an important method for treatment of malignant solid tumors in children. The mobilization and collection of blood stem cells is crucial for APBSCT. This study aimed to evaluate the clinical efficacy of mobilization and collection of blood stem cells by CIE or IEV chemotherapy protocol in APBSCT in children with neuroblastoma (NB) or rhabdomyosarcoma., Methods: The protocols of CIE (cisplatin, etoposide) and IEV (vincristine, dosfamide, etoposide) were used as mobilization chemotherapy in 8 cases of NB with stage IV and 3 cases of rhabdomysacoma with stage III, respectively. The results of the mobilization of blood stem cells were observed., Results: Of the 11 cases, mononuclear cells (MNC) and CD34+ cells were successfully collected and the volume of MNC and CD34 averaged (5.55 ± 1.43)× 10(8)/kg and (4.88 ± 2.48) × 10(6)/kg, respectively. No severe complications were observed during the mobilization and collection. A rapid hemopoietic reconstitution was observed in 10 children after APBSCT. One with NB out of the 10 children died of left heart failure 32 days after APBSCT. Others (9 cases) showed a nearly normal result of routine peripheral blood test 60 days after APBSCT., Conclusions: CIE or IEV protocol is effective and safe for the mobilization and collection of peripheral blood stem cells in children with NB or rhabdomysacoma.
- Published
- 2011
217. [General therapy of pediatric malignant germinoma: experience of 7 cases].
- Author
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Zhang WL, Zhang Y, Huang DS, Hong L, Wang YZ, Liu AP, Li J, and Zhou Y
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- Child, Child, Preschool, Combined Modality Therapy, Female, Germinoma blood, Humans, Infant, Male, alpha-Fetoproteins analysis, Germinoma therapy
- Published
- 2011
218. [Treatment of flatfoot deformity with concurrent spinal disease in children].
- Author
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Song WD, Li D, Liu SL, Huang JR, Shen HY, Huang DS, and Ma RF
- Subjects
- Adolescent, Child, Child, Preschool, Female, Humans, Male, Retrospective Studies, Flatfoot complications, Flatfoot surgery, Spinal Diseases complications
- Abstract
Objective: To study the curative effect of operation to flatfoot deformity with concurrent spinal disease in children., Methods: A retrospective survey was performed in 8 flatfoot patients with concurrent spinal disease from January 2004 to January 2008. Every case received special operative treatment. After casting, achilles tendon prolongation, reefing or tendon transfer, rehabilitation therapy was performed sequentially. Radiographs and clinical outcomes were evaluated during the follow-up., Results: Six patients were followed up. There were 2 feet in 1 female and 7 feet in 5 males. The complications included tethered cord syndrome (n = 1), T12-S1 vertebral canal arachnoid cyst (n = 1), cerebral palsy and scoliosis (n = 1) and scoliosis and spinal bifida occult (n = 3). The average age was 8.4 (4 - 14) years old and the average follow-up time 22 (14 - 64) months. All cases were satisfied with the appearance and function according to Maryland foot score. Five were excellent, 2 good and 2 fair postoperatively versus 2 fair and 7 failed preoperatively. The total excellent and good rate was 77.8%. In the early stage after tendon transfer in 4 patients, the lateral view of height arch, talus-first metatarsal angle, calcaneus-navicular-first metatarsal angle and talonavicular coverage angle improved. Yet it lost the improved angle and reverted to the pre-operative state. No degenerative change was detected in tarsus joints during the long follow-up., Conclusion: The special and sequential surgical procedure may be an effective regimen in the treatment of flatfoot deformity with concurrent spinal disease in children.
- Published
- 2010
219. [Therapeutic effects of high dose chemotherapy combined with autologous peripheral blood stem cell transplantation for neural ectodermal solid tumor originated from neural crest in children].
- Author
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Zhang WL, Zhang Y, Huang DS, Wang YZ, Zhu X, Hong L, Li P, Zhang PW, and Zhou Y
- Subjects
- Antigens, CD34 analysis, Antineoplastic Agents therapeutic use, Combined Modality Therapy, Female, Follow-Up Studies, Humans, Male, Peripheral Blood Stem Cell Transplantation, Transplantation, Autologous, Neural Crest pathology, Neuroblastoma therapy, Neuroectodermal Tumors, Primitive therapy
- Abstract
Objective: To investigate the efficacy of high dose chemotherapy combined with autologous peripheral blood stem cell transplantation (APBSCT) for the treatment of neural ectodermal solid tumor originated from neural crest in children., Methods: Twenty-three children at a medium age of 5.8 + or - 3.5 years with neural ectodermal solid tumor originated from neural crest were enrolled. Of the 23 children, 20 with stage IV neuroblastoma (9 were in complete remission, 7 were in partial remission and 4 were in progressive disease), 2 with stage IV primitive neuroectodermal tumor (PNET) in complete remission, and 1 with retinoblastoma in partial remission. Before APBSCT the children received 8.0 + or - 4.3 courses of chemotherapy. During chemotherapy the autologous peripheral blood stem cells were harvested and the tumor excision was performed. Then APBSCT was performed., Results: The reconstruction of the hematopoietic system was noted in 19 of 20 children with stage IV neuroblastoma 16.5 + or - 0.9 days after transplantation. A follow-up (median 15.8 months) was done in these children. The follow-up showed that the survival rate in children in complete remission before transplantation was 100%, 57% in those in partial remission, and none of children in progressive disease survived (P<0.05). The total survival rate was 67% in children with neuroblastoma. The child with retinoblastoma had complete remission in a 6-months follow-up. The tumors recurred in children with PNET 5 to 8 months after transplantation and all died within one year after transplantation., Conclusions: High dose chemotherapy combined with APBSCT can result in a good outcome in children with neural ectodermal solid tumor originated from neural crest in complete remission before transplantation and can improve the outcome in patients in partial remission before transplantation. However, the children with PNET, even in complete remission before transplantation, do not respond to the therapy.
- Published
- 2010
220. Agenesis of the gallbladder with adipose tissue in the gallbladder fossa.
- Author
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Zheng XY, Shen GL, Sun XD, and Huang DS
- Subjects
- Adipose Tissue diagnostic imaging, Cholecystography, Gallbladder diagnostic imaging, Humans, Laparoscopy, Male, Middle Aged, Tomography, X-Ray Computed, Ultrasonography, Adipose Tissue abnormalities, Gallbladder abnormalities
- Abstract
Agenesis of the gallbladder with normal bile ducts is a rare anomaly. More cases have been reported preoperatively because of the increased awareness of this entity. However some cases remain difficult to diagnosis. We report such a case. A symptomatic male patient (age 45) was diagnosed with chronic atrophic cholecystitis and cholecystlithiasis by ultrasonography and computed tomography scan. The images revealed solid tissue in the gallbladder fossa. However, during laparoscopic exploration no gallbladder or ectopic gallbladder was found. Only adipose tissue and the gallbladder artery were identified during the operation. Gallbladder agenesis was diagnosed postoperatively. It still remains a challenge to determine surgical intervention in these cases.
- Published
- 2010
221. [Role of B7-H1 in pancreatic carcinoma immune evasion].
- Author
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Huang DS, Liu JW, Geng L, Jiang GP, Shen GL, and Yao WF
- Subjects
- B7-H1 Antigen, Humans, Antigens, CD immunology, Immune Evasion, Interleukin-10 immunology, Pancreatic Neoplasms immunology
- Abstract
Objective: To investigate the role of B7-H1 expression in IL-10 production, the B7-H1 and IL-10 expression levels in pancreatic carcinoma tissues and to analyze the correlation between B7-H1 expression and IL-10 level., Methods: The mRNA and protein levels expressions of B7-H1 and IL-10 in 35 cases of pancreatic cancer and corresponding paracarcinoma tissues and 5 cases of normal pancreas tissues were detected by RT-PCR, Western blot and immunohistochemistry respectively., Results: The findings for the first time provided the evidences that there was a clear trend for B7-H1 and IL-10 expressions to be most highly expressed in carcinoma tissue, intermediately expressed in paracarcinoma tissue, and expressed at the lowest level in normal pancreatic tissue at mRNA and protein levels. Moreover, there were statistically significant differences in B7-H1 and IL-10 expression between pancreatic carcinoma tissues, corresponding paracarcinoma tissues and normal pancreatic tissues at mRNA and protein levels (P < 0.05). Furthermore, the immunohistochemistry indicated that there were high expression levels of B7-H1 (60.5% +/- 12.7%) and IL-10 (65.3% +/- 16.2%) in pancreatic carcinoma tissues while there were no significant expressions in normal pancreatic tissues. Meanwhile, correlation analysis revealed that B7-H1 expression was significant associated with IL-10 level in tumor tissues at mRNA (P = 0.008, r = 0.841) and protein levels (P = 0.007, r = 0.838)., Conclusions: Over-expression of B7-H1 may be responsible for the increasing IL-10 production in pancreatic cancer, which caused reduced immune response to tumor cells and contributed to pancreatic carcinoma escape from immune attack.
- Published
- 2009
222. [Association of 86 bp variable number tandem repeat polymorphism of interleukin-1 receptor antagonist gene with lumbar disc disease].
- Author
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Ye W, Huang DS, Chen WJ, Li CH, Peng Y, Liang AJ, and Liu SL
- Subjects
- Adult, Asian People genetics, Case-Control Studies, Female, Genome-Wide Association Study, Humans, Intervertebral Disc Degeneration genetics, Male, Middle Aged, Young Adult, Interleukin 1 Receptor Antagonist Protein genetics, Minisatellite Repeats, Polymorphism, Genetic, Spinal Diseases genetics
- Abstract
Objective: To investigate the association of 86 bp variable number tandem repeat (VNTR) polymorphism of interleukin-1 receptor antagonist (IL-1Ra) gene with lumbar disc disease and intervertebral disc degeneration., Methods: The 86 bp VNTR polymorphism of IL-1Ra gene was analyzed with PCR and electrophoresis for 81 patients with lumbar disc disease and 101 volunteers without sciatica (control). The grade of intervertebral disc degeneration was determined with magnetic resonance imaging, and the association of 86 bp VNTR polymorphisms with lumbar disc disease and intervertebral disc degeneration in those younger than 45 years was assessed., Results: The presence of 86bp VNTR polymorphisms of IL-1Ra gene was detected in both patients with lumbar disc disease and the control subjects. The distribution of 86 bp VNTR polymorphisms of IL-1Ra gene showed no significant difference between the two groups, but the distributions of 1/1, 1/2 and 2/2 or 1, 2 genotypes differed significantly. The current data did not support a significant association between the distribution of IL-1Ra gene 86bp VNTR polymorphism and lumbar disc degeneration., Conclusions: IL-1Ra gene 86bp VNTR polymorphism is present among Chinese population in association with lumbar disc disease, but not with lumbar disc degeneration.
- Published
- 2007
223. [Association of single nucleotide polymorphisms of IL-1b with lumbar disc disease].
- Author
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Ye W, Ma RF, Su PQ, Huang DS, Liu SL, Chen WJ, and Wang XG
- Subjects
- Adult, Female, Gene Frequency, Genetic Predisposition to Disease genetics, Genotype, Humans, Intervertebral Disc metabolism, Intervertebral Disc pathology, Intervertebral Disc Displacement pathology, Male, Middle Aged, Interleukin-1beta genetics, Intervertebral Disc Displacement genetics, Polymorphism, Single Nucleotide genetics
- Abstract
This study was to explore the relationships between (-511)T>C and (+3954)C>T single nucleotide polymorphisms(SNP) in IL-1beta gene with lumbar intervertebral disc disease. We analyzed (-511)T>C and (+3954)C>T SNP in IL-1beta gene by the polymerase chain reaction-restriction fragment length polymorphism and electrophoresis methods respectively in 81 cases with lumbar disc disease and 101 healthy controls. The relationship between (-511)T>C and (+3954)C>T SNP in IL-1beta gene and lumbar disc disease in two groups was measured, so does the relationship between (-511)T>C and (+3954)C>T SNP in IL-1beta gene and intervertebral disc degeneration in those younger than 45-year-old. The results showed there were (-511)T>C and (+3954)C>T SNP in IL-1beta gene. There was a significant difference in the distribution of TT, TC and CC genotype or T, C genotype of (-511)T>C of IL-1beta in two groups. And there was no significant difference in the distribution of (+3954)C>T SNP in IL-1beta gene in two groups. There was no significant difference between the distribution of (-511)T>C and (+3954)C>T SNP in IL-1beta gene and intervertebral disc degeneration in those younger than 45-year-old. It suggested (-511)T>C SNP in IL-1beta gene be one of the susceptible alleles for Lumbar disc disease.
- Published
- 2007
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224. [Effect of interleukin-6 on the chondrocytes in the cartilage endplate of rabbits in vitro].
- Author
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Ye W, Ma RF, Ding Y, Huang DS, Chen WJ, Peng Y, and Liu SL
- Subjects
- Aggrecans genetics, Animals, Cell Cycle drug effects, Cell Proliferation drug effects, Chondrocytes cytology, Chondrocytes metabolism, Collagen Type II genetics, Female, Gene Expression Regulation drug effects, Male, RNA, Messenger genetics, RNA, Messenger metabolism, Rabbits, Cartilage cytology, Chondrocytes drug effects, Interleukin-6 pharmacology
- Abstract
Objective: To evaluate the effect of interleukin-6 (IL-6) on the biological behaviors of the chondrocytes in the cartilage endplate of rabbits., Methods: Chondrocytes isolated from the cartilage endplate of New Zealand rabbits, verified for their biological characteristics by such means as toluidine blue staining for type II collagen, were treated with IL-6 at different concentrations. The proliferation of the chondrocytes was evaluated by MTT assay at different time points following the treatment, the cell cycle changes were determined by flow cytometry and the changes of aggrecan and type II collagen mRNAs detected by RT-PCR., Results: At the concentrations of 10, 50 and 100 ng/ml, IL-6 did not obviously affect the rate of chondrocyte proliferation. IL-6 at 50 ng/ml resulted in no obvious changes of the cell cycle of the chondrocytes, but significantly decreased the expression of collagen IIa mRNA., Conclusion: IL-6 has no effect on the proliferation and cell cycle of the chondrocytes, but at higher concentrations, it inhibits matrix synthesis of the chondrocytes to promote intervertebral disc degeneration.
- Published
- 2007
225. [The vitamin D receptor gene Tru I polymorphisms and its effect on the detection of Bsm I polymorphisms in Han nationality].
- Author
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Chen WJ, Ye W, Su PQ, Ding Y, Peng Y, Liang AJ, Huang DS, and Li GT
- Subjects
- Adult, Aged, Alleles, China ethnology, Female, Genotype, Humans, Male, Middle Aged, Asian People genetics, Deoxyribonucleases, Type II Site-Specific metabolism, Ethnicity genetics, Polymorphism, Genetic, Receptors, Calcitriol genetics
- Abstract
Objective: To explore the polymorphisms of vitamin D receptor (VDR) gene Tru I polymorphisms and the influence of this variation on Bsm I polymorphisms in Han nationality., Methods: Venous blood samples from 80 healthy individuals of Han nationality were collected and genomic DNA was extracted, VDR Bsm I and Tru I were tested by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) to analyze the polymorphisms of VDR gene; After using another primers to test VDR Bsm I in the same samples, the consistence of each method was assessed., Results: The frequencies of the VDR Tru I genotype in the groups were: TT 68.7%, Tt 26.3%, tt 5.0%; VDR Bsm I were: BB 6.2%, Bb 52.5%, bb 41.3%; Both polymorphisms were under Hardy-Weinberg equilibrium. After using another pair of primer, the frequencies of Bsm I genotype were BB 20.0%, Bb 26.2%, bb 53.8%, 22 genotype Bb changed to genotype BB or genotype bb in comparison with the result of first detection., Conclusion: The VDR Tru I polymorphism is found in the Han nationality, the distribution of this site's polymorphism is different from that of other nationalities. The presence of Tru I variation can result in some allele of Bsm I genotype drop-out in some study.
- Published
- 2007
226. [Long-term effect of high dose chemotherapy combined with stem cell transplantation on stage IV neuroblastoma in children].
- Author
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Tang SQ, Huang DS, Wang JW, Feng C, and Yang G
- Subjects
- Antineoplastic Combined Chemotherapy Protocols adverse effects, Child, Child, Preschool, Combined Modality Therapy, Female, Humans, Male, Neoplasm Staging, Neuroblastoma pathology, Transplantation, Autologous, Antineoplastic Combined Chemotherapy Protocols therapeutic use, Neuroblastoma therapy, Peripheral Blood Stem Cell Transplantation
- Abstract
Objective: Neuroblastoma is a highly malignant tumor. Stage IV neuroblastoma has a very poor long-term outcome by conventional chemotherapy and surgery and better therapies are essential. This study aimed to explore the long-term effect of high dose induction chemotherapy combined with autologous peripheral blood stem cell transplantation and 13-cis retinoid acid treatment on stage IV neuroblastoma in children., Methods: Twenty-eight children with stage IV neuroblastoma, aged 2.1-11.5 years (mean 3.3 +/- 1.9 years), were employed for the study. Primary sites of the tumors included adrenal (n=23), chest (n=3), chest-abdomen (n=1) and sacrum (n=1). Before autologous peripheral blood stem cell transplantation the patients received 6 courses of intensive induction chemotherapy. During chemotherapy the autologous peripheral blood stem cells were harvested and the tumor excision was done. After transplantation the local radiation and 13-cis retinoid acid therapy were administered., Results: After 6 courses of induction chemotherapy 13 patients got complete remission (CR), 11 got partial remission (PR), and 4 had no response. The 24 patients who received CR or PR completed the full therapy. A 3.5 +/- 0.7 years follow-up showed that the 4-year event-free survival of the CR and PR patients was 29.2%. The median no-relapse survival time in CR patients was 4.1 +/- 0.7 years but 2.8 +/- 0.5 years in PR patients (t= 3.9, P < 0.01)., Conclusions: High dose chemotherapy combined with autologous peripheral stem cell transplantation and 13 cis-retinoid acid treatment can improve the long-term outcome of patients with stage IV neuroblastoma. The patients in CR before transplantation had better outcomes than those in PR.
- Published
- 2006
227. [The application of METRx microendoscopes diskectomy system in the treatment of far lateral lumbar disc herniation].
- Author
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Li CH, Liu SL, Huang DS, Ding Y, and He JM
- Subjects
- Adult, Female, Follow-Up Studies, Humans, Male, Microsurgery, Middle Aged, Treatment Outcome, Diskectomy methods, Endoscopy, Intervertebral Disc Displacement surgery, Lumbar Vertebrae surgery
- Abstract
Objective: To introduce the METRx microendoscopes diskectomy system in the treatment of far lateral lumbar disc herniation., Methods: Fourteen cases of far lateral lumbar disc herniation were operated with METRx from February 1999 to December 2002. Among them, the average age was 49 years old (range 41 - 55 years old), male in 10 cases, female in 4 cases. All cases were single disc herniation; L(4), 5 herniation in 6 discs, L(5)-S(1) herniation in 8 discs; foraminal disc herniation in 6 cases, extra-foraminal disc herniation in 8 cases., Results: All the cases were followed up from 12 to 46 months (average 26.5 months) with the results of excellence in 10 cases, good in 3 cases, fair in 1 case and no failure case. There were no disc infection, dura laceration, nerve root injury and herniation recurrence., Conclusions: METRx is suitable for far lateral lumbar disc herniation with the advantages of minimal invasive, complete decompression of nerve root and rapid recovery. The correct approach and precise surgical technique are the key points for this operation.
- Published
- 2006
228. [The risk factors and preventive strategies of heterotopic ossification after artificial disc replacement in lumbar spine].
- Author
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Huang DS, Liang AJ, Ye W, Su PQ, Liu SL, Ma RF, Liang BL, and Li GZ
- Subjects
- Adult, Aged, Diskectomy, Female, Follow-Up Studies, Humans, Male, Middle Aged, Ossification, Heterotopic etiology, Retrospective Studies, Risk Factors, Lumbar Vertebrae surgery, Ossification, Heterotopic prevention & control, Prosthesis Implantation adverse effects
- Abstract
Objective: To analyze the risk factors of heterotopic (HO) ossification after total lumbar disc replacement and probe the preventive strategies for it., Methods: The radiographs and clinical data of 78 discs in 65 patients who received artificial lumbar disc replacement (ADR) from April 1998 to December 2003 were analyzed retrospectively by two radiologists and one orthopaedic surgeon and then postoperative HO were graded according to McAfee system. The bony formations in disc spaces, time of HO were found, and range of motion (ROM) of the operated levels were measured on radiographic films. In addition, the risk factors such as preoperative peri-annulus ossification, bony endplates injuries, and subsided or mal-position of the prostheses were also analyzed by Logistic regression analysis., Results: Postoperative HO was found in 10 spaces of 9 cases. Class I of HO were occurred in 7 patients at means 2.1 years postoperatively with normal range of motion preserved. Three of them turned into class II or III with 10 degrees of mean ROM in the following 2.5 years. Another 2 (2/9) cases with preoperative peri-annulus ossification had bridging trabecular bone (class III) between the endplates and 9 degrees of ROM 2 years after surgery, then turned into class IV at 6 years with 0 degrees and 4 degrees of motion in the operated levels. As the risk factors of HO, preoperative annulus ossification (2 cases), bony endplates injuries (5 cases), mal-positioned prostheses (2 cases) and subsided prostheses (2 cases) were found simultaneity with significant positive relation to HO occurred (P < 0.05)., Conclusions: Factors such as preoperative ossification of annulus, endplate injuries, prosthesis subsided and mal-position would have higher risks to have HO occurred after ADR, but ROM of most affected levels are preserved. Strict control indication and avoid all above risk factors can prevent HO occurring effectively.
- Published
- 2006
229. [NS398 induced apoptosis in pancreatic carcinoma cell strain BxPC-3 through a COX-2-in dependent pathway].
- Author
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Huang DS, Xu X, Zheng SS, and Cheng JF
- Subjects
- Caspase 3 drug effects, Cyclooxygenase 1 metabolism, Cyclooxygenase 2 metabolism, Humans, Pancreatic Neoplasms enzymology, Tumor Cells, Cultured, Apoptosis drug effects, Caspase 3 metabolism, Cyclooxygenase Inhibitors pharmacology, Nitrobenzenes pharmacology, Pancreatic Neoplasms pathology, Sulfonamides pharmacology
- Abstract
Objective: To investigate the effects of the selective cyclooxygenase-2 (COX-2) inhibitor NS398 on the growth of human pancreatic tumor BxPC-3 cell strain and its possible mechanisms., Methods: The effect of NS398 on cell growth was assessed by 3- (4,5-dimethylthiazol-2-yl) -2, 5-diphenyl thiazolyl blue (MTT) assay. Apoptosis was determined by fluorescence-activated cell scanning (FACS) analysis and assessment of the floating cell/attached cell ratio. Caspase-3 activation was evaluated by Active Caspase-3 Apoptosis Kit with flow cytometry. Reverse transcriptase-polymerase chain reaction analysis (RT-PCR) and Western blot were used to demonstrate expression levels of COX-1, COX-2 mRNA, and protein, as well as Caspase-3 protein in pancreatic tumor BxPC-3 cell strain., Results: Selective COX-2 inhibitor NS398 significantly decreased cell viability and induced apoptosis in pancreatic tumor BxPC-3 cell strain. The protein expression of Caspase-3 was induced by high-concentration NS398. Caspase-3 activity was strongly activated by NS398., Conclusions: Selective COX-2 inhibitor NS398 has antiproliferative and proapoptotic potential in pancreatic tumor BxPC-3 cells. Such effect is independent of COX-2, but correlates with Caspase-3 activation.
- Published
- 2005
230. [Long-term results of 84 surgically treated patients with extrahepatic bile duct carcinoma].
- Author
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Zheng SS, Qin YS, Liang TB, Huang DS, Zhang M, Wang WL, Shen Y, and Wang JH
- Subjects
- Adenocarcinoma mortality, Adult, Aged, Aged, 80 and over, Bile Duct Neoplasms mortality, Biliary Tract Surgical Procedures methods, Biliary Tract Surgical Procedures mortality, Female, Follow-Up Studies, Humans, Male, Middle Aged, Neoplasm Metastasis, Prognosis, Retrospective Studies, Survival Analysis, Treatment Outcome, Adenocarcinoma surgery, Bile Duct Neoplasms surgery, Bile Ducts, Extrahepatic surgery
- Abstract
Objective: Extrahepatic bile duct carcinoma is a rare but dismal malignacy. This study is conducted to show retrospective review and analysis of the correlation between the prognosis and different treatment modalities., Methods: The data of 84 such patients treated by different modalities from January, 1992 to July, 2000 were retrospectively reviewed and analyzed using SPSS 10.0 statistical package. The survivals were estimated by the Kaplan-Meier method and the difference among groups was tested by the log-rank test. The prognostic factors were determined by Cox multivariate analysis., Results: Of the 84 patients, 33 had complete resection, 19 palliative resection, 12 exploration alone, and the remaining 20 were treated by chemotherapy and/or radiotherapy. The mean follow-up time was 592 days. The overall 5-year survival rate was 13.1%. The 1-, 3- and 5-year survival rate following complete resection was 76.8%, 52.6% and 30.5% respectively, which was significantly higher than those of palliative surgery or chemotherapy/radiotherapy (P < 0.01). Multivariate analysis revealed that lymph node status (P = 0), histopathological grade (P = 0.001) and distant metastasis (P = 0.002) were significant high risk factors., Conclusion: The prognosis of extrahepatic bile duct carcinoma remains poor even after complete resection as shown to have a 5-year survival of 30.5%. More effective adjuvant therapy is needed. Extended resection may be helpful in improving the prognosis for carefully selected patients. Early diagnosis and early treatment is still the key to improve the long-term survival of extrahepatic bile duct carcinoma.
- Published
- 2005
231. [Mesenchymal chondrosarcoma in the pelvic cavity: a case report].
- Author
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Gao XN, Tang SQ, and Huang DS
- Subjects
- Child, Chondrosarcoma, Mesenchymal pathology, Diagnosis, Differential, Humans, Male, Pelvic Neoplasms pathology, Tomography, X-Ray Computed, Treatment Outcome, Chondrosarcoma, Mesenchymal diagnosis, Chondrosarcoma, Mesenchymal drug therapy, Pelvic Neoplasms diagnosis, Pelvic Neoplasms drug therapy
- Published
- 2005
232. [Design and experiment of the pedicle aiming device for the cervical spine].
- Author
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Huang DS, Su PQ, Ma RF, Li CH, Peng Y, and Liu SL
- Subjects
- Adult, Cervical Vertebrae diagnostic imaging, Female, Humans, Male, Spinal Fusion instrumentation, Spinal Fusion standards, Stereotaxic Techniques, Therapy, Computer-Assisted, Tomography, X-Ray Computed, Bone Screws, Cervical Vertebrae surgery, Internal Fixators, Orthopedic Fixation Devices, Spinal Fusion methods
- Abstract
Objective: To probe the accuracy and safety of using an aiming device in the transpedicular fixation of cervical spine., Methods: Eight cervical specimens were obtained. We used the computed tomography (CT) to scan C3 to C7, measured the medial angle of the pedicle, and determined the location of the pedicle projecting on the articular process. Then we took the oblique X-ray film, measured the cephalic/caudal angle of the pedicle, and determined the location of the pedicle projecting on the articular process. All the specimens were equally divided into two groups. Screws of 2.8 mm x 30 mm, were used. Specimens in one group were inserted with the transpedicular screw manually, while specimens in the other one inserted with the transpedicular screw using a self-designed aiming device that can be modulate at the three dimensions according to the angles of the pedicles., Results: The first group totally had 40 screws from C3 to C7. There were 13 screws in the pedicle, 9 violated the walls of the pedicle but not involved the adjunct structure, and 18 injured the important structure such as spinal cord, verteberal artery, or nerve root. In the other group, only 4 screws violated the walls of the pedicle but not involved the adjunct structure, and the others all in the pedicles. The difference was of statistical significance (P < 0.01)., Conclusion: In the cervical spine, transpedicular fixation using an aiming device can improve the accuracy and safety during operation.
- Published
- 2005
233. [Aplastic anemia transformed into acute myeloblastic leukemia M1 8 years later: a case report].
- Author
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Yang G, Tang SQ, Huang DS, Wang JW, Liu Y, and Wang JY
- Subjects
- Adolescent, Humans, Male, Anemia, Aplastic complications, Leukemia, Myeloid, Acute etiology
- Published
- 2005
234. [The role of artificial disc replacement in restoring lumbar segment motion and intervertebral height].
- Author
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Liu SL, Huang DS, Liang AJ, Ye W, Li CH, and Ma RF
- Subjects
- Adult, Female, Follow-Up Studies, Humans, Intervertebral Disc physiology, Joint Prosthesis, Male, Middle Aged, Prostheses and Implants, Range of Motion, Articular, Spinal Fusion adverse effects, Diskectomy, Intervertebral Disc surgery, Intervertebral Disc Displacement surgery, Lumbar Vertebrae surgery, Recovery of Function physiology
- Abstract
Objective: To investigate the role of artificial disc replacement (ADR) in restoring lumbar segment motion and intervertebral height (IH)., Methods: Seventy-five patients with intervertebral disc diseases underwent artificial disc replacement and were divided into 3 groups to be followed up for 2 years (group A, n = 32), 3 years (group B, n = 27), and 5 years (group C, n = 19) respectively. Before the operation and during the follow-up the range of motion (ROM) of the involved lumbar segments and IH were assessed., Results: The mean ROM and IH of the group A were 15 degrees and 14.1 mm respectively during the follow-up 2 years after operation. The mean ROM and IH of the group B were 14.2 degrees and 13.8 mm respectively during the follow-up 2 years after the operation and 13.7 degrees and 13.4 mm respectively during the follow-up 3 years after operation. The mean ROM and IH of the group C were 9.1 degrees and 11.5 mm respectively during the follow-up 2 years after the operation and 8.8 degrees and 11.0 mm respectively during the follow-up 3 years after operation, and 8.0 degrees and 10.3 mm respectively during the follow-up 5 years after operation. No significant differences in ROM and IH were found among different groups at any time point (all P > 0.05). Malposition was found in immediately after operation in 11 patients, all of the group C. During the follow-up, 3 cases of prostheses subsidence and 3 cases of annular ossification were found. No severe complications appeared., Conclusion: ADR is effective in restoring the ROM and IH of vertebrae suffering from intervertebral disc diseases.
- Published
- 2005
235. [Comparative study of vertebral body stress distribution following insertion of artificial lumbar intervertebral disc].
- Author
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Xu YC, Liu SL, Zhang MC, Huang DS, and Wang QY
- Subjects
- Diskectomy, Finite Element Analysis, Humans, Intervertebral Disc surgery, Lumbar Vertebrae surgery, Models, Biological, Prosthesis Implantation, Stress, Mechanical, Intervertebral Disc physiology, Lumbar Vertebrae physiology, Prostheses and Implants
- Abstract
Objective: To study vertebral body stress distribution of normal disc, post-Diskectomy and artificial disk respectively by 3-D finite element methods, and to explore artificial intervertebral disk insertion impact on stress distribution of vertebral body., Methods: Models of normal disk, post-Diskectomy, artificial disk and L(4 - 5) motion segment were established by using finite element software MSC. MARK, then vertebral body stress was analyzed through model of L(4 - 5) motion segment respectively., Results: The vertebral body's stress was the smallest after insertion of artificial intervertebral disk (AID), and its stress distributed equally. But the stress under post-discectomy was bigger than the normal disc's in all the motion state. On the other hand, the stress distribution state of the post-discectomy changed while the spine were in different motion state, during the spine flexion, the stress in the anterior of vertebral body was the biggest; While extension, in the posterior and in right flexion state, the biggest stress was in the right. While vertical compression and rotation, the stress distributed equally., Conclusion: The results illustrate that the vertebral body's stress is the smallest after insertion of AID in 3 groups of all motion state, and its stress distributes equally. But the level of vertebral body stress increases after discectomy comparing with the normal group. In generally, it is much more reasonable that the disc is reconstructed with AID because of the biomechanical effect on vertebral body made by AID insertion.
- Published
- 2004
236. [Effect of chemical modification of grafts on the survival improvement post haploidentical bone marrow transplantation in mice].
- Author
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Yang G, Tang SQ, Zhang XF, Liu LZ, Huang DS, and Wang JW
- Subjects
- Animals, Bone Marrow immunology, Disease Models, Animal, Graft vs Host Disease immunology, Graft vs Host Disease prevention & control, HLA Antigens immunology, Immunosuppressive Agents therapeutic use, Male, Mice, Mice, Inbred BALB C, Polyethylene Glycols therapeutic use, T-Lymphocytes immunology, Bone Marrow drug effects, Bone Marrow Transplantation immunology, Graft Survival immunology, Immunosuppressive Agents pharmacology, Polyethylene Glycols pharmacology, T-Lymphocytes drug effects
- Abstract
Objective: Human leukocyte antigen (HLA) haploidentical bone marrow is a potential source of donor to children for its availability. The drawback is deleterious graft versus host disease (GVHD) reaction post transplantation because of the incompatibility of HLA antigen expression between donors and recipients, in which donor T lymphocyte is stimulated to proliferate and differentiate. The methoxy polyethylene glycol (mPEG) is a kind of amphoteric compound without immunogenicity, which was used to modify various proteins covalently and to prepare the versatile blood type. If mPEG modification blocks the activation of T cells in grafts, GVHD reaction probably would become less serious and transplantation might become successful. The aim of this study was to verify the improvement of haploidentical bone marrow transplantation (BMT) in a murine model by using mPEG of certain concentration to modify the grafts., Methods: Male BALB/c mice were chosen as the donor, and female CB(6)F(1) mice as the recipient. There were three groups of mPEG modification, non-modification and irradiation control, and 20 mice in each group. The modified and non-modified mixture of bone marrow and spleen cells (as T lymphocytes) were transplanted to haploidentical lethally irradiated CB(6)F(1) mice via the tail vein. After the transplant, the hematopoietic recovery, survival rate, acute graft versus host disease (aGVHD) and chromosomal karyotype were analyzed and compared with controls., Results: Seventy-five percent (15/20) of mice survived in the group of mPEG modification, while only 40% (8/20) survived in the group without the modification (chi(2) = 5.01, P = 0.025). And 100% mice died in the group of the irradiation control within 2 weeks. The hematopoietic recovery in the group of mPEG modification was show n to be faster than that in the group without modification (P < 0.05). Histopathological examination of the skin, liver and intestine showed typical signs of aGVHD, but the GVHD grading in the group of modification was less severe. The recipient mice in both groups of transplantation surviving for more than 75 days showed complete donor-type implantation by the chimerism examination., Conclusion: The modification of grafts by mPEG could alleviate aGVHD and improve the survival rate of mice after the haploidentical bone marrow transplantation.
- Published
- 2004
237. [Treatment of high-risk neuroblastoma with intensive chemotherapy, autologous peripheral blood stem cell transplantation, and 13-cis-retinoic acid].
- Author
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Tang SQ, Huang DS, Wang JW, Zhang XF, Liu LZ, Yu F, and Yang G
- Subjects
- Bone Marrow Cells metabolism, Child, Preschool, Female, Follow-Up Studies, Humans, Male, Treatment Outcome, Abdominal Neoplasms therapy, Isotretinoin therapeutic use, Neuroblastoma therapy, Peripheral Blood Stem Cell Transplantation
- Abstract
Objective: The prognosis for neuroblastoma in advanced stage is still poor, even under conventional chemotherapy. This study aimed to investigate if very high dose chemotherapy in conjunction with autologous peripheral blood stem cell transplantation and 13-cis-retinoic acid could get excellent results in children with high risk neuroblastoma., Methods: Six children, aged from 4 to 8 years, with stage IV neuroblastoma were included in the study. The duration of the illness before admission was 1 to 12 months. Primary sites of the diseases were in the abdominal cavity (n = 5) and thoracic cavity (n = 1). All of patients had bone marrow metastasis, and one had multiple bone metastasis and orbital metastasis. All of the patients received very high dose chemotherapy, surgery, local radiation (20-30 Gy), and autologous peripheral blood stem cell transplantation as well as 13-cis retinoic acid. Induction chemotherapy included vincristine 0.67 mg/(m2 x 24 h, x 3), cyclophosphamide 2.1 g/(m2 x 24 h, x 2) and doxorubicin 25 mg/(m2 x 24 h, x 3) for 4 courses. Drugs were given as 24 hour-continuous intravenous infusion. Etopside 200 mg/(m2 x 24 h, x 3) and cisplatin 50 mg/(m2 x 24 h, x 3) were given for 2 courses. Conditioning regimen included carboplatin 400 mg/(m2.d) for 4 days, etoposide 300 mg/(m2.d) for 4 days and melphalan 70 mg/(m2.d) for 3 days. 13-cis retinoic acid 160 mg/(m2.d) started on +59 days for 6 courses, each course including 14 days therapy and 14 days rest., Results: Six patients got a complete response before stem cell transplantation. Their bone marrow metastasis disappeared and so did bone and orbital metastasis. However, marrow suppression due to very high dose chemotherapy occurred in all of the patients, which lasted for 3-4 weeks for peripheral leukocyte recovery. Fever occurred after they finished 1/3 course of chemotherapy. Infection, however, was cured with the use of Fortum and Imipenem, ect. Autologous peripheral blood stem cell transplantation was initiated and successful in all cases. Follow-up studies revealed that all the patients were in CR status 4-18 months after transplant, and the cardiac and liver and renal functions were normal. Meanwhile, bone marrow was recovered or in the process of recovery., Conclusion: The new strategies focused on very high dose chemotherapy, autologous peripheral blood stem cell transplantation and biological therapy might be a good option for patients with advance neuroblastoma.
- Published
- 2004
238. [Two cases of Askin tumor misdiagnosed as pulmonary tuberculosis].
- Author
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Huang DS, Tang SQ, Wang JW, Liu L, and Lu S
- Subjects
- Adolescent, Antineoplastic Combined Chemotherapy Protocols therapeutic use, Carcinoma, Small Cell diagnosis, Carcinoma, Small Cell drug therapy, Child, Diagnosis, Differential, Humans, Male, Neuroectodermal Tumors, Primitive, Peripheral drug therapy, Neuroectodermal Tumors, Primitive, Peripheral therapy, Prognosis, Thoracic Neoplasms drug therapy, Treatment Outcome, Diagnostic Errors, Neuroectodermal Tumors, Primitive, Peripheral diagnosis, Thoracic Neoplasms diagnosis, Tuberculosis, Pulmonary diagnosis
- Published
- 2004
239. [Early diagnosis of pancreatic cancer is essential to improving the effect of treatment].
- Author
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Zheng SS, Huang DS, Li QY, Liang TB, Wang WL, Shen Y, Zhang M, Wu J, Xu X, and Yu J
- Subjects
- Adolescent, Adult, Aged, Aged, 80 and over, Child, Early Diagnosis, Female, Follow-Up Studies, Humans, Male, Middle Aged, Neoplasm Staging, Pancreas pathology, Pancreas surgery, Pancreatectomy, Prognosis, Retrospective Studies, Survival Analysis, Treatment Outcome, Young Adult, Pancreatic Neoplasms diagnosis, Pancreatic Neoplasms surgery
- Abstract
Objectives: To explore the relation between tumor TNM of pancreatic cancer and treatment, and to summarize the experience in diagnosis and treatment of pancreatic cancer., Methods: The data on 321 patients with pancreatic cancer treated at our hospital between January 1990 and December 2000 were reviewed retrospectively. Radical resection and survival for various TNM stages were analyzed., Results: The rates of radical resection for stage I, stage II, stage III, stage IVA, and stage IVB were 100%, 100%, 82.6%, 12.3% and 2.1% respectively. The 1-, 3- and 5-year survivals of stage I, II were 73.3%, 40.0% and 30.0% respectively, and higher than those of other groups., Conclusions: The patients with pancreatic cancer diagnosed early can undergo radical resection. The elevation of early diagnosis rate is significant for the improvement of survival.
- Published
- 2003
240. Expression of 4-1BB molecule on peripheral blood T cells in liver transplanted patients and its clinical implication.
- Author
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Wan YL, Zheng SS, Jia CK, Liang TB, Huang DS, Wang WL, Li MW, and Zhao ZC
- Subjects
- Adult, Antigens, CD genetics, Female, Flow Cytometry, Gene Expression immunology, Humans, Male, Middle Aged, Receptors, Nerve Growth Factor genetics, Receptors, Tumor Necrosis Factor genetics, Reverse Transcriptase Polymerase Chain Reaction, Tumor Necrosis Factor Receptor Superfamily, Member 9, Antigens, CD immunology, Liver Transplantation immunology, Receptors, Nerve Growth Factor immunology, Receptors, Tumor Necrosis Factor immunology, T-Lymphocytes immunology
- Abstract
Objective: To investigate the gene expression of 4-1BB in peripheral blood mononuclear cells (PBMCs) and its possible significance in clinical liver transplantation., Methods: Reverse transcription-polymerase chain reaction (RT-PCR) was used to determine the gene expression of 4-1BB in PBMCs from 22 patients receiving liver transplantation, 13 patients with primary liver carcinoma (PLC), and 12 healthy controls. To determine whether 4-1BB molecule is also expressed on the surface of CD4+ and CD8+ T cell, flow cytometry was used to analyse the phenotype of T cell subsets from the blood of liver transplantation patients., Results: 4-1BB mRNA was detected in PBMCs from stable survivors after liver transplantation, but almost not detected in PBMCs from PLC patients and healthy controls. Meanwhile, 4-1BB was almost not expressed on the surface of CD4+ and CD8+T cells in healthy controls and PLC patients. A low level of 4-1BB expression, however, was found on the surface of CD4+ and CD8+ T cells from the stable survivors after liver transplantation., Conclusions: This study demonstrates that although patients are stable after liver transplantation, effector T-cells can also be activated through the signal of 4-1BB molecule and persistent immune response to grafts. Blockage of 4-1BB/4-1BBL pathway may benefitially reduce the clinical dosage of immunosuppressive agents and prolong the survival of grafts.
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- 2003
241. [Clinical analysis of different periods of liver transplantation at an organ transplantation centre].
- Author
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Liang TB, Zheng SS, Wang WL, Huang DS, Shen Y, and Zhang M
- Subjects
- Adult, Female, Hepatitis B prevention & control, Humans, Male, Middle Aged, Postoperative Complications prevention & control, Recurrence, Retrospective Studies, Liver Transplantation mortality
- Abstract
Objective: To summarize our clinical experience in liver transplantation while considering the background in this field in China., Methods: Ninety-five patients who had received liver transplantation from April 1993 to March 2002 were analyzed retrospectively. Three periods were defined objectively as period I (1993 - 1997), II (1999) and III (2000 - 2002). Operative techniques, recipients, original diseases, complications and survival rates were compared among the three periods., Results: Malignant liver lesions were the main cause for liver transplantation in period I and II. The ratio of number of malignant disease to total recipients decreased gradually from period I to III (100%, 53% and 35%, respectively). The 1-year survival rate in patients with benign liver disease was 85% and the total operative mortality was 5% in period III. The incidence of hepatitis B virus reactivation or reinfection was 24% twelve months after liver transplantation. Vascular complication decreased but biliary complications did not and remained a major long-standing problem. No veno-venous bypass technique was used in period III, and its advantages were obvious when comparing with those with veno-venous bypass in period I and II., Conclusions: Strict selection of recipients, fine operative technique, familiarity with various complications and correct therapeutic methods, prophylaxis of recurrence of hepatitis B and hepatocellular carcinoma are necessary to improve long-term results of liver transplantation in China.
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- 2003
242. Clinical experience in liver transplantation from an organ transplantation center in China.
- Author
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Zheng SS, Liang TB, Wang WL, Huang DS, Shen Y, Zhang M, Xu X, and Mou LJ
- Subjects
- Adult, Biliary Tract Diseases etiology, China, Female, Hepatitis B epidemiology, Humans, Incidence, Liver Diseases surgery, Liver Neoplasms surgery, Male, Middle Aged, Postoperative Period, Recurrence, Retrospective Studies, Survival Analysis, Vascular Diseases etiology, Liver Transplantation adverse effects
- Abstract
Objective: To sum up the experience in liver transplantation in a period of ten years at a single center., Methods: We retrospectively reviewed the clinical records of 120 patients receiving liver transplantation from April 1993 to October 2002. The patients' clinical characteristics, surgical techniques, complications and survival were compared in the phases of 1993-1997 (phase I), 1999 (phase II), and 2000-2002 (phase III)., Results: Malignant liver diseases were major indications for liver transplantation in phase I (100%) and II (53.3%), but decreased markedly in percentage in phase III (34.0%). When compared with recipients in phase I and II, the survival of recipients with benign liver diseases in phase III was significantly improved with the 3-month, 6-month and 1-year survival rates of 85.7%, 84.5% and 83.1%, respectively. For patients with malignant liver diseases, the 3-month, 6-month and 1-year survival rates were 87.4%, 81.1% and 46.0%, respectively. The reinfection rate of hepatitis B virus was 24% 12 months after transplantation. With technical refinements, the incidence of postransplantation vascular complications has significantly decreased from 29.4% in phase I and II to 4.9% in phase III. Biliary complications remained one of the major obstacles to long-term survival. No veno-venous bypass was applied in phase III, providing a promising outcome., Conclusion: Strict selection of potential recipients, technical refinement, appropriate management of vascular and biliary complications, and prophylaxis of recurrences of hepatitis B and malignant liver diseases are important to obtain long-term survival of patients receiving liver transplantation in China.
- Published
- 2002
243. [Alteration of P73 and P51 genes and its significance in human gastric carcinogenesis]
- Author
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Huang DS and Xie HY
- Abstract
OBJECTIVE: To evaluate the relatinship between the expression of P51, P73 and the oncogenesis and development of human gastric carcinoma. METHODS: The expression of P73 mRNA were detected both in 32 human gastric carcinoma tissues and adjacent normal gastric tissues by reverse transcriptase polymerase chain reaction (RT-PCR). RESULTS: Overexpressions of P73 mRNA were found in 17/32 gastric carcinoma tissues,in 2/32 adjacent normal gastric tissues.The positive expression rate of P73 mRNA in gastric carcinooma tissues was significantly higher than in adjacent normal gastric tissues( P<0.01). However, a significant correlation was found between the positive expression rate of P73 mRNA in gastric carcinoma tissues and the TNM staging(P<0.05). THe low expressions of P51A mRNA and P51B were found in all gastric carcinoma tissues and adjacent normal gastric tissues. The expression of P51A in gastric carcinoma tissues were much higher than adjacent normal gastric tissues (P<0.05). The expression of P51B is no significant correlation was observed between gastirc carcinoma tissues and adjacent normal gastric tissues. CONCLUSION: The results suggest that there is an overexpression odf P73 and P51A mRNA in gastric cancer tissues, and their expressions is relationship with oncogenesis and developnment of gastric carcinoma.
- Published
- 2002
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244. Functional changes of the heart and lung in perioperative period of orthotopic liver transplantation.
- Author
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Zheng SS, Lu AW, Huang DS, Chen QL, and Xu LZ
- Subjects
- Adult, Blood Pressure, Cardiac Output, Female, Hemodynamics, Humans, Male, Middle Aged, Postoperative Period, Pulmonary Artery physiopathology, Pulmonary Wedge Pressure, Vascular Resistance, Heart physiopathology, Liver Transplantation, Lung physiopathology
- Abstract
Objective: To investigate the variation of functions of the heart and lung during orthotopic liver transplantation (OLT)., Methods: Pulmonary artery cannula and right radial artery cannula were indewelled before anaesthesia and the parameters of hemodynamics in different periods were monitored. Analysis of variance was used to reveal the variation among the groups. T hypothesis test in paired data was used to compare the preoperative parameters with those in each period during operation and after operation respectively, and to compare the parameters immediately after operation with those in each period after operation respectively., Results: During the operation, heart rate increased, but blood pressure decreased significantly at the beginning of no-liver period, increased again in a short period and then increased progressively 12 h after operation. Pulmonary artery pressure (PAP) increased from before the no-liver period to 60 h after operation. Pulmonary wedge pressure changed in accordance with the variation of PAP. Cardiac output was maintained at a high-output level from before operation to 60 h after operation. Systemic vascular resistance (SVR) was within the normal limits before operation, whereas pulmonary vascular resistance (PVR) was lower than normal. In the no-liver period during the operation, SVR decreased significantly. Both SVR and PVR increased progressively and returned to normal postoperatively., Conclusions: The patients undergoing OLT have a high cardiac output and low resistance obstacle before and during the operation, and will recover gradually after operation. Monitoring hemodynamics during the peri-operative period is of significance in the prevention and treatment of pneumonedema and cardiac functional insufficiency.
- Published
- 2002
245. Prophylaxis and treatment of hepatitis B virus reinfection following liver transplantation.
- Author
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Zheng SS, Wu J, Liang TB, Wang WL, Huang DS, and Xu X
- Subjects
- 2-Aminopurine therapeutic use, Adult, Antiviral Agents therapeutic use, DNA, Viral blood, Dose-Response Relationship, Drug, Drug Therapy, Combination, Famciclovir, Hepatitis B diagnosis, Hepatitis B Core Antigens blood, Hepatitis B Surface Antigens blood, Hepatitis B e Antigens blood, Hepatitis B virus genetics, Hepatitis B virus immunology, Humans, Lamivudine administration & dosage, Male, Reverse Transcriptase Inhibitors administration & dosage, Secondary Prevention, 2-Aminopurine analogs & derivatives, Hepatitis B drug therapy, Hepatitis B prevention & control, Lamivudine therapeutic use, Liver Transplantation, Postoperative Complications drug therapy, Postoperative Complications prevention & control, Reverse Transcriptase Inhibitors therapeutic use
- Abstract
Objectives: To prevent and early diagnose hepatitis B virus reinfection and recurrent hepatitis B following liver transplantation, and to discuss the further treatment of recurrent hepatitis B., Methods: Liver transplantation recipients received lamivudine for prophylaxis of HBV reinfection. Virological and biochemical data, serum HBV DNA, and immunohistological staining for HBsAg and HBcAg in liver biopsy specimens were tested in due time., Results: Five patients with hepatitis B virus reinfection and two patients with hepatitis B recurrence were observed after liver transplantation. One patient with recurrent hepatitis B developed chronic severe hepatitis B despite treatment. One patient improved after a series of treatment., Conclusions: Hepatitis B virus reinfection or recurrent hepatitis B following liver transplantation occurs mostly 6-12 months after operation. The diagnosis of hepatitis B recurrence should be taken into account when liver biochemical data becomes poor during this period. The treatment for recurrent hepatitis B after liver transplantation includes increased dosage of lamivudine, application of famciclovir, and other liver protection measures.
- Published
- 2002
246. Living related liver transplantation for an infant with biliary atresia.
- Author
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Zheng SS, Huang DS, Wang WL, Liang TB, Zhang M, Shen Y, Lu AW, Liao SY, and Xu X
- Subjects
- Adult, Bile metabolism, Drainage, Female, Humans, Infant, Male, Mothers, Treatment Outcome, Biliary Atresia surgery, Liver Transplantation adverse effects, Living Donors
- Abstract
Objective: To sum up the preliminary experience in living related liver transplantation (LRLT)., Methods: A 9-month-old male infant with biliary atresia (BA) who had undergone an unsuccessful Kasai operation was defined as a candidate for LRLT. The donor was his 30-year-old mother. Her lateral lobe of the left liver was transplanted into the infant's body as the graft. The left branches of the portal vein, left hepatic artery and left hepatic vein of the graft were end-to-end anastomosed to the portal vein, hepatic artery proper and hepatic vein of the recipient respectively. Biliary drainage was reestablished via Roux-en-Y operation., Results: The donor retained her liver function within 2 weeks after the operation. Steroid and FK506 were prescribed in immunosuppressive therapy for the recipient. The blood bilirubin level of the infant decreased to normal 2 weeks after operation. No acute rejection occurred. Biliary leakage in the early period after the transplantation was controlled by drainage, and E.coli infection was effectively treated with antibiotics. The donor and recipient are in satisfactory condition to the present., Conclusion: LRLT is advisable for children with biliary atresia.
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- 2002
247. Cytomegalovirus infection in liver transplant recipients.
- Author
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Fan J, Ma WH, Qian J, Chen Z, Huang DS, Wang WL, and Zheng SS
- Subjects
- Adult, Antibodies, Viral blood, Antigens, Viral genetics, Cytomegalovirus Infections epidemiology, DNA, Viral analysis, Female, Humans, Immunoglobulin G blood, Immunoglobulin M blood, Male, Postoperative Complications diagnosis, Postoperative Complications epidemiology, Postoperative Complications virology, Seroepidemiologic Studies, Cytomegalovirus Infections diagnosis, Liver Transplantation
- Abstract
Objective: To explore cytomegalovirus (CMV) infection in recipients of liver transplantation (LT)., Methods: 30 recipients of LT were screened for the appearance of CMV infection by using ELISA to test anti-CMV-Ab from serum samples and using immunohistochemistry method to test CMV antigen expression and nested-PCR to amplify CMV-DNA from blood samples., Results: Four of 243 samples taken from 30 recipients came out positive of anti-CMV IgG and anti-CMV IgM with a positive rate of 100% and 1.6% respectively. 85 samples resulted in CMV antigen expression (35.0%) with the average antigen index being 4.2+/-3.1/5 X 10(4) WBC. Besides, 99 samples were found to be positive by nested-PCR with a positive rate of 40.7%. 61 samples were found to be simultaneously positive in test of CMV antigen and DNA, with a rate of 25.1%., Conclusions: Infection of CMV is common in recipients of LT. Simultaneous screening of anti-CMV-Ab, CMV-Ag and CMV-DNA after liver transplantation is very important for early diagnosis of CMV infection.
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- 2002
248. Orthotopic liver transplantation in treatment of 77 patients with end-stage hepatic disease.
- Author
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Zheng SS, Huang DS, Wang WL, Liang TB, Zhang M, Shen Y, Xu X, Wu J, Yan S, Guo H, and Lu AW
- Subjects
- Adolescent, Adult, Child, Child, Preschool, Cholangiocarcinoma mortality, Cholangiocarcinoma surgery, Female, Follow-Up Studies, Humans, Infant, Liver Neoplasms mortality, Liver Neoplasms surgery, Male, Middle Aged, Retrospective Studies, Liver Failure mortality, Liver Failure surgery, Liver Transplantation mortality
- Abstract
Objective: To summarize the experience of human orthotopic liver transplantation (OLTx) in treatment of patients with end-stage hepatic disease and their perioperative management., Methods: A retrospective analysis was made on OLTx performed in 77 patients from April 1993 to September 2001 in our department included combined liver-kidney transplantation (6 patients) and living related liver transplantation (2). Among them, 76 were adults and 1 was infant (67 males and 10 females). The donors included 75 non-heart beating donors and 2 living related donors. Veno-venous bypass was used only in 45 cases during the operation. immunosuppressive agents consisted of cyclosporine, cellcept, ALG corticosteroids and FK506., Results: The one-year survival rates of grafts and recipients with benign hepatic disease were 75%. The recipients this year are surviving with good function of grafts., Conclusion: Liver transplantation is the best therapeutic method for a large number of patients with end-stage hepatic disease in China.
- Published
- 2002
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