299 results on '"Hua-Jun CHEN"'
Search Results
202. Brain structural and functional alterations in patients with unilateral hearing loss
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Jing Li, Hui Ji, Bin Liu, Zhichun Huang, Xu Feng, Lingling Zhang, Xin Zhu, Yuan Feng, Ming Yang, Jing-ya Chen, Hua-Jun Chen, and Gao-Jun Teng
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Adult ,Male ,medicine.medical_specialty ,Middle temporal gyrus ,Precuneus ,Audiology ,Hearing Loss, Unilateral ,Brain mapping ,Lingual gyrus ,Inferior temporal gyrus ,Memory ,medicine ,Image Processing, Computer-Assisted ,Limbic System ,Humans ,Language ,Auditory Cortex ,Brain Mapping ,medicine.diagnostic_test ,Brain morphometry ,Brain ,Middle Aged ,medicine.disease ,Magnetic Resonance Imaging ,Sensory Systems ,Temporal Lobe ,medicine.anatomical_structure ,nervous system ,Case-Control Studies ,Audiometry, Pure-Tone ,Female ,Pure tone audiometry ,Unilateral hearing loss ,Psychology ,Neuroscience - Abstract
Alterations of brain structure and functional connectivity have been described in patients with hearing impairments due to distinct pathogenesis; however, the influence of unilateral hearing loss (UHL) on brain morphology and regional brain activity is still not completely understood. In this study, we aim to investigate regional brain structural and functional alterations in patients with UHL. T1-weighted volumetric images and task-free fMRIs were acquired from 14 patients with right-sided UHL (pure tone average ≥ 40 dB HL) and 19 healthy controls. Hearing ability was assessed by pure tone audiometry. Voxel-based morphometry (VBM) was performed to detect brain regions with changed gray matter volume or white matter volume in UHL. The amplitude of low-frequency fluctuation (ALFF) was calculated to analyze brain activity at the baseline and was compared between two groups. Compared with controls, UHL patients showed decreased gray matter volume in bilateral posterior cingulate gyrus and precuneus, left superior/middle/inferior temporal gyrus, and right parahippocampal gyrus and lingual gyrus. Meanwhile, patients showed significantly decreased ALFF in bilateral precuneus, left inferior parietal lobule, and right inferior frontal gyrus and insula and increased ALFF in right inferior and middle temporal gyrus. These findings suggest that chronic UHL could induce brain morphological changes and is associated with aberrant baseline brain activity.
- Published
- 2014
203. Fully Automated Detection of Corticospinal Tract Damage in Chronic Stroke Patients
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Ya-ru Yang, Hua-Jun Chen, Yongmei Shi, Huijun Li, Xue-song Lu, Ming Yang, Bin Liu, and Gao-J. jun Teng
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Adult ,Male ,medicine.medical_specialty ,Pathology ,Article Subject ,Pyramidal Tracts ,lcsh:Computer applications to medicine. Medical informatics ,General Biochemistry, Genetics and Molecular Biology ,Brain Ischemia ,Pattern Recognition, Automated ,Automation ,Atrophy ,Region of interest ,Internal medicine ,medicine ,Image Processing, Computer-Assisted ,Humans ,Diagnosis, Computer-Assisted ,Stroke ,Aged ,Pyramidal tracts ,General Immunology and Microbiology ,medicine.diagnostic_test ,Applied Mathematics ,Motor Cortex ,Magnetic resonance imaging ,General Medicine ,Middle Aged ,medicine.disease ,Magnetic Resonance Imaging ,medicine.anatomical_structure ,Diffusion Tensor Imaging ,Motor Skills ,Modeling and Simulation ,Corticospinal tract ,Cardiology ,lcsh:R858-859.7 ,Female ,Psychology ,human activities ,Biomarkers ,Diffusion MRI ,Motor cortex ,Research Article - Abstract
Structural integrity of the corticospinal tract (CST) after stroke is closely linked to the degree of motor impairment. However, current methods for measurement of fractional atrophy (FA) of CST based on region of interest (ROI) are time-consuming and open to bias. Here, we used tract-based spatial statistics (TBSS) together with a CST template with healthy volunteers to quantify structural integrity of CST automatically. Two groups of patients after ischemic stroke were enrolled, group 1 (10 patients, 7 men, and Fugl-Meyer assessment (FMA) scores⩽50) and group 2 (12 patients, 12 men, and FMA scores = 100). CST of FAipsi, FAcontra, and FAratiowas compared between the two groups. Relative to group 2, FA was decreased in group 1 in the ipsilesional CST (P<0.01), as well as the FAratio(P<0.01). There was no significant difference between the two subgroups in the contralesional CST (P=0.23). Compared with contralesional CST, FA of ipsilesional CST decreased in group 1 (P<0.01). These results suggest that the automated method used in our study could detect a surrogate biomarker to quantify the CST after stroke, which would facilitate implementation of clinical practice.
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- 2014
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204. Classification of cirrhotic patients with or without minimal hepatic encephalopathy and healthy subjects using resting-state attention-related network analysis
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Pei-Cheng Li, Xi-Qi Zhu, Hua-Jun Chen, Gao-Jun Teng, and Yu Wang
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Liver Cirrhosis ,Male ,Pathology ,Epidemiology ,Precuneus ,lcsh:Medicine ,Brain mapping ,Diagnostic Radiology ,Functional Magnetic Resonance Imaging ,Medicine and Health Sciences ,Attention ,Clinical Epidemiology ,lcsh:Science ,Hepatic encephalopathy ,Default mode network ,Brain Mapping ,Multidisciplinary ,Cognitive Neurology ,Liver Diseases ,Radiology and Imaging ,Brain ,Discriminant Analysis ,Middle Aged ,Magnetic Resonance Imaging ,medicine.anatomical_structure ,Cirrhosis ,Neurology ,Cardiology ,Female ,Research Article ,Adult ,medicine.medical_specialty ,Rest ,Cognitive Neuroscience ,Neuroimaging ,Gastroenterology and Hepatology ,Sensitivity and Specificity ,Text mining ,Diagnostic Medicine ,Internal medicine ,Task-positive network ,medicine ,Humans ,Resting state fMRI ,business.industry ,lcsh:R ,Biology and Life Sciences ,Inferior parietal lobule ,medicine.disease ,Case-Control Studies ,Hepatic Encephalopathy ,lcsh:Q ,Nerve Net ,business ,human activities ,Neuroscience - Abstract
BACKGROUND: Attention deficit is an early and key characteristic of minimal hepatic encephalopathy (MHE) and has been used as indicator for MHE detection. The aim of this study is to classify the cirrhotic patients with or without MHE (NMHE) and healthy controls (HC) using the resting-state attention-related brain network analysis. METHODS AND FINDINGS: Resting-state fMRI was administrated to 20 MHE patients, 21 NMHE patients, and 17 HCs. Three attention-related networks, including dorsal attention network (DAN), ventral attention network (VAN), and default mode network (DMN), were obtained by independent component analysis. One-way analysis of covariance was performed to determine the regions of interest (ROIs) showing significant functional connectivity (FC) change. With FC strength of ROIs as indicators, Linear Discriminant Analysis (LDA) was conducted to differentiate MHE from HC or NMHE. Across three groups, significant FC differences were found within DAN (left superior/inferior parietal lobule and right inferior parietal lobule), VAN (right superior parietal lobule), and DMN (bilateral posterior cingulate gyrus and precuneus, and left inferior parietal lobule). With FC strength of ROIs from three networks as indicators, LDA yielded 94.6% classification accuracy between MHE and HC (100% sensitivity and 88.2% specificity) and 85.4% classification accuracy between MHE and NMHE (90.0% sensitivity and 81.0% specificity). CONCLUSIONS: Our results suggest that the resting-state attention-related brain network analysis can be useful in classification of subjects with MHE, NMHE, and HC and may provide a new insight into MHE detection.
- Published
- 2014
205. Monolayer-molybdenum-disulfide-based nano-optomechanical transistor and tunable nonlinear responses
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Chen Changzhao, Hua-Jun Chen, Xian-Wen Fang, and Yang Li
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Kerr effect ,Physics::Optics ,Nanotechnology ,02 engineering and technology ,01 natural sciences ,law.invention ,Resonator ,chemistry.chemical_compound ,law ,0103 physical sciences ,Nano ,Monolayer ,Materials Chemistry ,Electrical and Electronic Engineering ,010306 general physics ,Molybdenum disulfide ,Physics ,business.industry ,Transistor ,021001 nanoscience & nanotechnology ,Condensed Matter Physics ,Electronic, Optical and Magnetic Materials ,Semiconductor ,chemistry ,Optical transistor ,0210 nano-technology ,business - Abstract
Atomically thin two-dimensional semiconductor nanomaterials have attained considerable attention currently. Here, we present a nano-optomechanical system based on a suspended monolayer molybdenum disulfide (MoS 2 ). The linear and nonlinear coherent optical properties of this system, and the phenomenon of phonon-induced transparency are demonstrated. The transmission of the probe field can be manipulated by the power of a second ‘gating' (pump) field, which indicates a promising candidate for an optical transistor. We further study the nonlinear effect of the system, and the optical Kerr effect of the monolayer MoS 2 resonator can be regulated under different parameter regimes. This scheme proposed here may indicate potential chip-scale applications of monolayer MoS 2 resonator in quantum information with the currently popular pump-probe technology.
- Published
- 2016
206. Response
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Gao-Jun, Teng, Hua-Jun, Chen, and Xi-Qi, Zhu
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Male ,Brain Mapping ,Hepatic Encephalopathy ,Brain ,Humans ,Female ,Cognition Disorders ,Magnetic Resonance Imaging - Published
- 2013
207. Feasibility of computed tomography-guided core needle biopsy in producing state-of-the-art clinical management in Chinese lung cancer
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Hua-Jun, Chen, Jin-Ji, Yang, Liang-Yi, Fang, Min-Min, Huang, Hong-Hong, Yan, Xu-Chao, Zhang, Chong-Rui, Xu, and Yi-Long, Wu
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Original Articles - Abstract
A satisfactory biopsy determines the state-of-the-art management of lung cancer in this era of personalized medicine. This study aimed to investigate the suitability and efficacy of computed tomography (CT)-guided core needle biopsy in clinical management.A cohort of 353 patients with clinically suspected lung cancer was enrolled in the study. Patient factors and biopsy variables were recorded. Epidermal growth factor receptor (EGFR) gene mutations and echinoderm microtubule-associated protein-like 4 (EML4)-anaplastic lymphoma kinase (ALK) rearrangement were detected in tumor specimens. Adequacy of biopsic obtainment for clinical trial screening and tissue bank establishment were reviewed.Overall diagnostic accuracy of malignancy achieved 98.5%. The median biopsy time of the cohort was 20 minutes. In patients with non-small cell lung cancer (NSCLC), 99.3% (287/289) were diagnosed as specific histologic subtypes, and two patients (0.7%) were determined as NSCLC not otherwise specified (NOS). EGFR mutations were analyzed in 81.7% (236/289) of patients with NSCLC, and 98.7% (233/236) showed conclusive results. EML4-ALK gene fusion was tested in 43.9% (127/289) of NSCLC patients, and 98.4% (125/127) showed conclusive results: 6.4% (8/125) of those had gene fusion. Ninety-six NSCLC patients participated in clinical trial screening and provided mandatory tumor slides for molecular profiling. Pathological evaluation was fulfilled in 90 patients (93.8%); 99.4% (320/322) of patients with malignancy provided extra tissue for the establishment of a tumor bank.CT-guided core needle biopsy provided optimal clinical management in this era of translational medicine. The biopsic modality should be prioritized in selected lung cancer patients.
- Published
- 2013
208. Sensitivity to epidermal growth factor receptor tyrosine kinase inhibitors in males, smokers, and non-adenocarcinoma lung cancer in patients with EGFR mutations
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Zhu Zeng, Hua-Jun Chen, Yi-Long Wu, Xu-Chao Zhang, Jin-Ji Yang, and Hong-Hong Yan
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0301 basic medicine ,Adult ,Male ,Cancer Research ,Lung Neoplasms ,Clinical Biochemistry ,medicine.disease_cause ,Pathology and Forensic Medicine ,Cohort Studies ,03 medical and health sciences ,0302 clinical medicine ,Carcinoma, Non-Small-Cell Lung ,medicine ,Carcinoma ,Humans ,In patient ,Survival rate ,Protein Kinase Inhibitors ,Aged ,Retrospective Studies ,Aged, 80 and over ,Mutation ,business.industry ,Smoking ,Histology ,Middle Aged ,medicine.disease ,Prognosis ,respiratory tract diseases ,ErbB Receptors ,Survival Rate ,030104 developmental biology ,Treatment Outcome ,Oncology ,030220 oncology & carcinogenesis ,Cancer research ,Adenocarcinoma ,Female ,business ,Tyrosine kinase ,Epidermal growth factor receptor tyrosine kinase - Abstract
Introduction The demographical/clinical characteristics of being Asian, having an adenocarcinoma, being female, and being a “never-smoker” are regarded as favorable predictors for epidermal growth factor receptor ( EGFR) tyrosine kinase inhibitor (TKI) efficacy in non-small cell lung cancer (NSCLC) with unknown EGFR gene status. In this study, we examined the effects of the supposedly unfavorable clinical variables in EGFR-mutant patients. Method In total, 159 EGFR-mutant NSCLC patients' clinical features were correlated with progression-free survival (PFS), response rate (RR), and overall survival (OS). Multivariate analysis of clinical characteristics was performed using the Cox and logistic regression methods. Result There were 90 females (56.6%), 112 never-smokers (70.4%), and 153 patients with adenocarcinomas (96.2%). All patients were treated with EGFR-TKI, and 52.8% received TKI in a first-line setting. The median PFS of patients receiving first-line TKI was similar, regardless of gender (males vs females: 9.1 vs 9.7 months, p=0.793), smoking status (never-smokers vs smokers: 9.9 vs 9.1 months, p=0.570), or histology (adenocarcinoma vs non-adenocarcinoma: 9.7 vs 9.2 months, p=0.644). OS curves of first-line TKI-treated patients were also not associated with gender (p=0.722), smoking status (p=0.579), or histology (p=0.480). Similar results of PFS and OS were obtained for patients who received TKI beyond first-line. Multivariate analysis indicated that none of these clinical factors was an independent predictor of survival. Conclusions The supposedly ‘favorable’ clinical factors of female gender, non-smoking status, and adenocarcinoma were not independent predictive factors for PFS or OS in this population of EGFR-mutant NSCLC patients.
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- 2013
209. Effect of preparation methods on morphology of active manganese dioxide and 2,4-dinitrophenol dsorption performance.
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Hua-jun Chen, Wenjie Tian, and Wuxiu Ding
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MANGANESE dioxide ,DINITROPHENOL ,SORPTION ,ADSORPTION (Chemistry) ,FREUNDLICH isotherm equation - Abstract
Herein active manganese dioxide was prepared by different methods using KMnO
4 and MnSO4 · H2 O as inorganic precursors. The impact of preparation methods on morphology and adsorption performance of the synthesized products was investigated. The experimental results show that the reaction temperature and pressure had a great effect on the morphology and adsorption performance of active manganese dioxide. The shape of active manganese dioxide prepared at room temperature and pressure was short rod-like while active manganese dioxide synthesized by hydrothermal method was mesoporous fibers and had better adsorption performance. The adsorption behavior of 2,4-dinitrophenol on mesoporous manganese dioxide was well described by Langmuir and Freundlich isotherm equation (R2 >0.99) and pseudo-secondorder kinetic equation (R2 >0.99), so the adsorption process maybe a chemical and monolayer adsorption. The pH of solution had significant effect on the adsorption performance of 2,4-dinitrophenol on mesoporous manganese dioxide. The adsorption capacity was 2.539 mg/g in the condition of pH=7. [ABSTRACT FROM AUTHOR]- Published
- 2018
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210. Majorana fermions detection based on a coupled quantum dot-nanomechanical resonator system
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Hua-Jun Chen, Changzhao Chen, Yang Li, and Xian-Wen Fang
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Physics ,Condensed matter physics ,Phonon ,Physics::Optics ,Fermion ,Condensed Matter::Mesoscopic Systems and Quantum Hall Effect ,Physics::History of Physics ,MAJORANA ,Quantum dot ,Qubit ,Quantum mechanics ,Quantum ,Quantum tunnelling ,Quantum computer - Abstract
Motivated by a recent experiment providing evidence for Majorana zero modes in iron (Fe) chains on the superconducting Pb surface, in the present work, an optical method for probing Majorana fermions is proposed theoretically, which is very different from the current tunneling spectroscopy experiments with electrical means. The optical detection proposal consists of a hybrid quantum dot-nanomechanical resonator system driven by one strong pump field and one weak probe field. With the optical means, the signal in the coherent optical spectrum indicates a distinct signature of Majorana fermions in the end of iron chains when the quantum dot coupled to Majorana fermions. In addition, an optical scheme for determining the coupling strength between Majorana fermions and the quantum dot is also introduced, which affords a straightforward method to measure such coupling. We further investigate the role of the nanomechanical resonator in the hybrid system. The vibration of the nanomechanical resonator behaving as a phonon cavity will enhance the exciton resonance spectrum, which is robust for the detection of Majorana fermions. This optical scheme affords a potential supplement to detecte Majorana fermions and supports to use Majorana fermions in Fe chains as qubits for potential applications in quantum computing devices.
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- 2016
211. Response to tyrosine kinase inhibitors in advanced non-small-cell lung cancer with concomitant c-MET overexpression and EGFR mutation
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Bin-Chao Wang, Xu-Chao Zhang, Qing Zhou, Yi-Long Wu, Xiao-Yan Bai, Jin-Ji Yang, Na Na Lou, Jian Su, Zhen Wang, and Hua-Jun Chen
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Cancer Research ,C-Met ,business.industry ,medicine.disease ,medicine.disease_cause ,respiratory tract diseases ,chemistry.chemical_compound ,Oncology ,chemistry ,Egfr mutation ,hemic and lymphatic diseases ,Concomitant ,Cancer research ,Medicine ,Non small cell ,KRAS ,business ,Lung cancer ,neoplasms ,Gene ,Tyrosine kinase - Abstract
9054Background: The common driver genes in non-small-cell lung cancer (NSCLC) include EGFR, ALK, KRAS and so on. MET amplification coexisting with EGFR mutation is recognized as intrinsic resistanc...
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- 2016
212. Molecular mechanism of transformation from adenocarcinoma to small-cell lung cancer after EGFR-TKI
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Hua-Jun Chen, Jin-Ji Yang, Bin-Chao Wang, Jie-Fei Han, Fei-Yu Niu, Zhi Xie, Jian Su, Zhen Wang, Qiuyi Zhang, Yi-Long Wu, Hai-Yan Tu, Li-Xu Yan, and Qing Zhou
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Cancer Research ,integumentary system ,biology ,business.industry ,Kinase ,medicine.disease ,respiratory tract diseases ,Transformation (genetics) ,Egfr tki ,Oncology ,Molecular mechanism ,Cancer research ,biology.protein ,Medicine ,Adenocarcinoma ,Non small cell ,Epidermal growth factor receptor ,business ,Lung cancer - Abstract
e20601Background: In patients with advanced non–small-cell lung cancer (NSCLC) harboring epidermal growth factor receptor (EGFR) activating mutations, EGFR-tyrosine kinase inhibitors (TKIs) are oft...
- Published
- 2016
213. White matter abnormalities correlate with neurocognitive performance in patients with HBV-related cirrhosis
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Yu Wang, Hua-Jun Chen, Gao-Jun Teng, Xi-Qi Zhu, Ying Cui, and Yu-Chen Chen
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Adult ,Male ,Pathology ,medicine.medical_specialty ,Statistics as Topic ,Neuropsychological Tests ,Corpus callosum ,Functional Laterality ,Lingual gyrus ,White matter ,Atrophy ,Imaging, Three-Dimensional ,Leukoencephalopathies ,Fractional anisotropy ,medicine ,Humans ,Brain ,Voxel-based morphometry ,Middle Aged ,medicine.disease ,Magnetic Resonance Imaging ,medicine.anatomical_structure ,Diffusion Magnetic Resonance Imaging ,nervous system ,Neurology ,Frontal lobe ,Hepatic Encephalopathy ,Female ,Neurology (clinical) ,Psychology ,Cognition Disorders ,Neurocognitive - Abstract
Background White matter (WM) abnormalities are common in cirrhotic patients and possibly contribute to hepatic encephalopathy (HE), a frequent neuropsychiatric complication of cirrhosis. However, little is known about these WM abnormalities and their relationship to neurocognitive deficits in patients with HBV-related cirrhosis. Methods Three-dimensional T1-weighted magnetic resonance imaging and diffusion tensor imaging (DTI) scans were obtained from 67 patients with HBV-related cirrhosis and 40 controls. Voxel-based morphometry and voxel-based DTI were performed to detect macroscopic atrophy and damage to the microstructural integrity of the WM, respectively. Correlation analyses were performed to investigate the relationships between WM abnormalities and neurocognitive performances. Results Patients with cirrhosis exhibited significantly decreased WM volume and fractional anisotropy (FA) values, especially in the corpus callosum, thalamus, extra-nuclear area, sensorimotor area, fusiform gyrus, lingual gyrus, and frontal lobes. These abnormalities were more severe with increasing Child–Pugh stage, minimal HE, and previous overt HE. Changes in the corpus callosum, frontal lobe, sensorimotor area, internal capsule, and temporal–occipital lobes were correlated with poor neurocognitive performance. Also, the significantly decreased global WM volume and mean FA value were correlated with poor neurocognitive performances. Conclusions Diffuse WM abnormalities are common in patients with HBV-related cirrhosis. Advanced liver disease and episodes of HE are two factors associated with WM abnormalities. The correlation between poor neurocognitive performance and WM abnormalities suggests that WM abnormalities may be one of mechanisms underlying neurocognitive deficits in patients with HBV-related cirrhosis.
- Published
- 2012
214. Aberrant resting-state corticostriatal functional connectivity in cirrhotic patients with hyperintense globus pallidus on T1-weighted MR imaging
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Ying Cui, Hua-Jun Chen, Gao-Jun Teng, Yu Wang, and Xi-Qi Zhu
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Adult ,Liver Cirrhosis ,Male ,Pathology ,medicine.medical_specialty ,Inferior frontal gyrus ,lcsh:Medicine ,Neuroimaging ,Gastroenterology and Hepatology ,Striatum ,Neuropsychological Tests ,Biology ,Globus Pallidus ,Brain mapping ,Diagnostic Radiology ,medicine ,Humans ,lcsh:Science ,Cerebral Cortex ,Brain Mapping ,Manganese ,Multidisciplinary ,medicine.diagnostic_test ,Resting state fMRI ,Cognitive Neurology ,Liver Diseases ,Putamen ,fMRI ,lcsh:R ,Middle Aged ,Magnetic Resonance Imaging ,Globus pallidus ,medicine.anatomical_structure ,Cirrhosis ,Neurology ,nervous system ,Cerebral cortex ,Medicine ,Female ,lcsh:Q ,Cognition Disorders ,Radiology ,Functional magnetic resonance imaging ,Research Article ,Neuroscience - Abstract
Neurobiological and neuroimaging studies have emphasized the structural and functional alterations in the striatum of cirrhotic patients, but alterations in the functional connections between the striatum and other brain regions have not yet been explored. Of note, manganese accumulation in the nervous system, frequently reflected by hyperintensity at the bilateral globus pallidus (GP) on T1-weighted imaging, has been considered a factor affecting the striatal and cortical functions in hepatic decompensation. We employed resting-state functional magnetic resonance imaging to analyze the temporal correlation between the striatum and the remaining brain regions using seed-based correlation analyses. The two-sample t-test was conducted to detect the differences in corticostriatal connectivity between 44 cirrhotic patients with hyperintensity at the bilateral GP and 20 healthy controls. Decreased connectivity of the caudate was detected in the anterior/middle cingulate gyrus, and increased connectivity of the caudate was found in the left motor cortex. A reduction in functional connectivity was found between the putamen and several regions, including the anterior cingulate gyrus, right insular lobe, inferior frontal gyrus, left parahippocampal gyrus, and anterior lobe of the right cerebellum; increased connectivity was detected between the putamen and right middle temporal gyrus. There were significant correlations between the corticostriatal connectivity and neuropsychological performances in the patient group, but not between the striatal connectivity and GP signal intensity. These alterations in the corticostriatal functional connectivity suggested the abnormalities in the intrinsic brain functional organiztion among the cirrhotic patients with manganese deposition, and may be associated with development of metabolic encephalopathy. The manganese deposition in nervous system, however, can not be an independent factor predicting the resting-state brain dysfunction in real time.
- Published
- 2012
215. Involvement of epidermal growth factor receptor overexpression in the promotion of breast cancer brain metastasis
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Fang, Nie, Jian, Yang, Song, Wen, Yan-Li, An, Jie, Ding, Sheng-Hong, Ju, Zhen, Zhao, Hua-Jun, Chen, Xin-Gui, Peng, Stephen T C, Wong, Hong, Zhao, and Gao-Jun, Teng
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ErbB Receptors ,Mice ,Brain Neoplasms ,Cell Movement ,Cell Line, Tumor ,Gene Knockdown Techniques ,Transplantation, Heterologous ,Animals ,Humans ,Mice, Nude ,Breast Neoplasms ,Female ,Cell Proliferation - Abstract
Brain-metastatic breast cancer (BMBC) is increasing and poses a severe clinical problem because of the lack of effective treatments and because the underlying molecular mechanisms are largely unknown. Recent work has demonstrated that deregulation of epidermal growth factor receptor (EGFR) may correlate with BMBC progression. However, the exact contribution that EGFR makes to BMBC remains unclear.The role of EGFR in BMBC was explored by serial analyses in a brain-trophic clone of human MDA-MB-231 breast carcinoma cells (231-BR cells). EGFR expression was inhibited by stable short-hairpin RNA transfection or by the kinase inhibitor erlotinib, and it was activated by heparin-binding epidermal growth factor-like growth factor (HB-EGF). Cell growth and invasion activities also were analyzed in vitro and in vivo.EGFR inhibition or activation strongly affected 231-BR cell migration/invasion activities as assessed by an adhesion assay, a wound-healing assay, a Boyden chamber invasion assay, and cytoskeleton staining. Also, EGFR inhibition significantly decreased brain metastases of 231-BR cells in vivo. Surprisingly, changes to EGFR expression affected cell proliferation activities less significantly as determined by a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, an anchorage-independent growth assay, and cell cycle analysis. Immunoblot analysis suggested that EGFR drives cells' invasiveness capability mainly through phosphoinositide 3-kinase/protein kinase B and phospholipase C γ downstream pathways. In addition, EGFR was involved less in proliferation because of the insensitivity of the downstream mitogen-activated protein kinase pathway.The current results indicated that EGFR plays more important roles in cell migration and invasion to the brain than in cell proliferation progression on 231-BR cells, providing new evidence of the potential value of EGFR inhibition in treating BMBC.
- Published
- 2011
216. Structural and functional cerebral impairments in cirrhotic patients with a history of overt hepatic encephalopathy
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Yi Zhang, Jie Ding, Ming Yang, Yu Wang, Hua-Jun Chen, Xi-Qi Zhu, Gao-Jun Teng, and Hao Shu
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Liver Cirrhosis ,Male ,Pathology ,medicine.medical_specialty ,Insular cortex ,Sensitivity and Specificity ,Atrophy ,Gyrus ,medicine ,Humans ,Radiology, Nuclear Medicine and imaging ,Hepatic encephalopathy ,Default mode network ,medicine.diagnostic_test ,Resting state fMRI ,business.industry ,Reproducibility of Results ,General Medicine ,Middle Aged ,medicine.disease ,Magnetic Resonance Imaging ,medicine.anatomical_structure ,Posterior cingulate ,Hepatic Encephalopathy ,Female ,Nervous System Diseases ,Functional magnetic resonance imaging ,business - Abstract
Objective Diffuse brain atrophy has been observed in cirrhotic patients and recent reports have revealed the persistence of cognitive impairment after clinical resolution of overt hepatic encephalopathy. We sought to explore the continued influence of overt hepatic encephalopathy on neurological function by measuring brain resting-state inherent connectivity, based on an investigation of structural abnormalities. Methods Neuropsychological tests and structural and functional magnetic resonance scanning were conducted in 20 healthy controls and 21 cirrhotic patients with a history of overt hepatic encephalopathy. The analysis of voxel-based morphometry and functional connectivity were performed to detect the alterations in brain structure and function, respectively. Results Patients showed significantly worse performance in neuropsychological tests as compared with controls, despite apparently normal mental status. Analysis of voxel-based morphometry revealed a decrease in gray matter volume primarily in the midline regions, bilateral insular cortex and caudates, left parahippocampal gyrus, and right cerebellum posterior lobe, while the volume of the bilateral thalamus showed an increase. Of these regions, the posterior cingulate cortex with peak atrophy was selected as the origin for the analysis of functional connectivity. Typical patterns of a default mode network were identified in both groups. Decreased functional connectivity was found in the medial prefrontal gyrus, left inferior parietal lobule, and left middle temporal gyrus in the patients. Conclusions Both functional and structural impairments were evident after apparent recovery from overt hepatic encephalopathy, demonstrating that brain dysfunction induced by hepatic encephalopathy persisted after clinical resolution and provided a basis for further evolution of the disease.
- Published
- 2011
217. Changes in the regional homogeneity of resting-state brain activity in minimal hepatic encephalopathy
- Author
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Yu Wang, Yi Zhang, Ming Yang, Xi-Qi Zhu, Gao-Jun Teng, Hua-Jun Chen, and Bin Liu
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Male ,medicine.medical_specialty ,Brain activity and meditation ,Rest ,Precuneus ,Brain mapping ,Cuneus ,Internal medicine ,medicine ,Humans ,Brain Mapping ,medicine.diagnostic_test ,Resting state fMRI ,General Neuroscience ,Brain ,Magnetic resonance imaging ,Middle Aged ,Adaptation, Physiological ,Magnetic Resonance Imaging ,medicine.anatomical_structure ,Hepatic Encephalopathy ,Cerebellar vermis ,Cardiology ,Female ,Nerve Net ,Psychology ,Functional magnetic resonance imaging ,human activities ,Neuroscience - Abstract
Resting-state functional magnetic resonance imaging (fMRI) has facilitated the study of spontaneous brain activity by measuring low-frequency oscillations in blood-oxygen-level-dependent signals. Analyses of regional homogeneity (ReHo), which reflects the local synchrony of neural activity, have been used to reveal the mechanisms underlying the brain dysfunction in various neuropsychiatric diseases. However, it is not known whether the ReHo is altered in cirrhotic patients with minimal hepatic encephalopathy (MHE). We recruited 18 healthy controls and 18 patients with MHE. The ReHo was calculated to assess the strength of the local signal synchrony. Compared with the healthy controls, the patients with MHE had significantly decreased ReHo in the cuneus and adjacent precuneus, and left inferior parietal lobe, whereas the regions showing increased ReHo in patients with MHE included the left parahippocampal gyrus, right cerebellar vermis, and bilateral anterior cerebellar lobes. We found a positive correlation between the mean ReHo in the cuneus and adjacent precuneus and the score on the digit-symbol test in the patient group. In conclusion, the analysis of the regional homogeneity of resting-state brain activity may provide additional information with respect to a clinical definition of MHE.
- Published
- 2011
218. Relative abundance of EGFR mutations predicts benefit from gefitinib treatment for advanced non-small-cell lung cancer
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Wen-Zhao Zhong, Bin-Chao Wang, Xue-Ning Yang, Jin-Ji Yang, Zhen Wang, Yi-Sheng Huang, Xu-Chao Zhang, Jian Su, Hong-Hong Yan, She-Juan An, Yi-Long Wu, Zhi-Hong Chen, Hua-Jun Chen, Chong-Rui Xu, Qing Zhou, and Xiao-Lu Yin
- Subjects
Adult ,Male ,Cancer Research ,Lung Neoplasms ,Antineoplastic Agents ,Bioinformatics ,medicine.disease_cause ,DNA sequencing ,Disease-Free Survival ,Gefitinib ,Carcinoma, Non-Small-Cell Lung ,medicine ,Carcinoma ,Humans ,Epidermal growth factor receptor ,Lung cancer ,Relative species abundance ,Protein Kinase Inhibitors ,Aged ,Aged, 80 and over ,Mutation ,biology ,Kinase ,business.industry ,Middle Aged ,medicine.disease ,ErbB Receptors ,Oncology ,Cancer research ,biology.protein ,Quinazolines ,Female ,business ,medicine.drug - Abstract
Purpose Our aim was to determine whether abundance of epidermal growth factor receptor (EGFR) mutations in tumors predicts benefit from treatment with EGFR–tyrosine kinase inhibitors (TKIs) for advanced non–small-cell lung cancer (NSCLC). Patients and Methods We detected EGFR mutations in 100 lung cancer samples using direct DNA sequencing and amplification refractory mutation system (ARMS). Mutation-positive tumors by both methods carried high abundance of EGFR mutations. Tumors that were mutation positive by ARMS but mutation negative by direct DNA sequencing harbored low abundance of EGFR mutations. Mutation-negative tumors by both methods carried wild-type EGFR. All patients received gefitinib treatment. The correlation between EGFR mutation abundance and clinical benefit from gefitinib treatment was analyzed. Results Of 100 samples, 51 and 18 harbored high and low abundances of EGFR mutations, respectively; 31 carried wild-type EGFR. Median progression-free survival (PFS) was 11.3 (95% CI, 7.4 to 15.2) and 6.9 months (95% CI, 5.5 to 8.4) in patients with high and low abundances of EGFR mutations, respectively (P = .014). Median PFS of patients with low abundance of EGFR mutations was significantly longer than that of those with wild-type tumors (2.1 months; 95% CI, 1.0 to 3.2; P = .010). Objective response rates (ORRs) were 62.7%, 44.4%, and 16.1%, and overall survival (OS) rates were 15.9 (95% CI, 13.4 to 18.3), 10.9 (95% CI, 2.7 to 19.1), and 8.7 months (95% CI, 4.6 to 12.7) for patients with high abundance of EGFR mutations, low abundance of EGFR mutations, and wild-type EGFR, respectively. The difference between patients with high and low abundances of EGFR mutations was not significant regarding ORR and OS. Conclusion The relative EGFR mutation abundance could predict benefit from EGFR-TKI treatment for advanced NSCLC.
- Published
- 2011
219. Polymer drawing in the unsymmetrical air flow of melt blowing: Numerical simulation
- Author
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Li-Li Wu, Ting Chen, Hua-Jun Chen, and Yao Ma
- Subjects
chemistry.chemical_classification ,business.product_category ,Materials science ,Computer simulation ,Renewable Energy, Sustainability and the Environment ,lcsh:Mechanical engineering and machinery ,Airflow ,unsymmetrical air flow field ,Polymer ,melt blowing ,Transverse plane ,chemistry ,polymer drawing ,Melt blowing ,Die (manufacturing) ,lcsh:TJ1-1570 ,Composite material ,business ,Displacement (fluid) - Abstract
The unsymmetrical air flow of the dual slot die in the melt blowing process is simulated. The diameter and transverse displacement of the threadline are obtained numerically. The result shows that the threadline diameter in the unsymmetrical air flow is larger than that in the symmetrical one. This paper helps to point out the research direction for producing finer melt blown fibers.
- Published
- 2014
220. Using geodata and geoprocessing web services in embedded device
- Author
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Yang Lv, Denghui Zhang, Le Yu, Bin Xie, and Hua-jun Chen
- Subjects
Database ,Computer science ,SOAP ,computer.internet_protocol ,business.industry ,Geoprocessing ,Navigation system ,Usability ,computer.software_genre ,GeneralLiterature_MISCELLANEOUS ,ComputingMethodologies_DOCUMENTANDTEXTPROCESSING ,Software system ,Web service ,business ,WS-Policy ,computer ,XML - Abstract
Using XML web services in embedded system could be able to use the existing geodata and geoprocessing services through wired/wireless network. However, the processing and memory limitations of embedded devices exacerbate the problem in the area of complexity, constraints, and the verbosity of the protocols used in XML web services effect the usability of XML. This paper presents a GPS navigation system, which accesses OGC-compliant geodata and geoprocessing services using gSOAP toolkit. This system composes of hardware platform, embedded operation system and software system. Our experimental result shows gSOAP could provides transparent SOAP API allows embedded system using XML web services.
- Published
- 2010
221. In vitro sequence-dependent synergism between paclitaxel and gefitinib in human lung cancer cell lines
- Author
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Qiu-Xiong Lin, Hua Cheng, Zhi-Hong Chen, Ailin Guo, Xu-Chao Zhang, She-Juan An, Hua-Jun Chen, Yi-Long Wu, Song Dong, and Yi-Fang Zhang
- Subjects
Cancer Research ,medicine.medical_specialty ,Lung Neoplasms ,Paclitaxel ,medicine.drug_class ,Toxicology ,Tyrosine-kinase inhibitor ,Drug Administration Schedule ,chemistry.chemical_compound ,Gefitinib ,Internal medicine ,Carcinoma, Non-Small-Cell Lung ,Cell Line, Tumor ,Antineoplastic Combined Chemotherapy Protocols ,Medicine ,Humans ,Pharmacology (medical) ,Epidermal growth factor receptor ,Phosphorylation ,Lung cancer ,neoplasms ,Pharmacology ,biology ,business.industry ,Cell growth ,Cell Cycle ,Cancer ,Drug Synergism ,Cell cycle ,medicine.disease ,respiratory tract diseases ,ErbB Receptors ,Endocrinology ,Oncology ,chemistry ,Cancer research ,biology.protein ,Quinazolines ,business ,medicine.drug ,Signal Transduction - Abstract
In clinical trials, the epidermal growth factor receptor tyrosine kinase inhibitor (EGFR-TKI) administered concomitantly with first-line cytotoxicity chemotherapy failed to confer a survival benefit to patients with non-small-cell lung cancer (NSCLC). The aim of this study was to test whether paclitaxel followed by gefitinib is superior to other treatment schedules of NSCLC cell lines and to clarify the underlying mechanisms. Human lung cancer cell lines with wild-type and mutant-type EGFR genes were used as in vitro models to define the differential effects of various schedules of paclitaxel with gefitinib treatment on cell growth, signaling pathway, and cell cycle distribution. Sequence-dependent antiproliferative effects differed between EGFR-TKI-resistant and EGFR-TKI-sensitive lung cancer cell lines. Exposure to paclitaxel resulted in an increased pEGFR level. This increase in phosphorylation was inhibited by subsequent exposure to gefitinib, whereas during the reverse sequence, the inhibition effect was reduced. After paclitaxel exposure, a higher level of pEGFR was observed in mitotic than in interphase cells. The sequence of paclitaxel followed by gefitinib resulted in greater anti-VEGF activity than did the reverse sequence. We confirmed that gefitinib arrested cells in G1, and paclitaxel arrested them in S phase. The sequence of paclitaxel followed by gefitinib arrested cells in G1, whereas the reverse sequence arrested cells in S and G2 phases. These findings suggest that the sequence of paclitaxel followed by gefitinib may be superior to other sequences in treating NSCLC cell lines. Our results also provide molecular evidence to support clinical treatment strategies for patients with lung cancer.
- Published
- 2010
222. [Effects of Dendrolimus punctatus feeding and methyl jasmonate (MeJA)--or terpenes fumigation on abscisic acid and jasmonic acid contents in Pinus massoniana seedling needles]
- Author
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Wen-Hong, Deng, Ying-Bai, Shen, Hua-Jun, Chen, Zhen-Yu, Li, and Xiang-Ning, Jiang
- Subjects
Plant Leaves ,Plant Growth Regulators ,Seedlings ,Terpenes ,Animals ,Cyclopentanes ,Oxylipins ,Acetates ,Moths ,Pinus ,Abscisic Acid - Abstract
Abscisic acid (ABA) and Jasmonic acid (JA) play an important role in inducing the stress-resistance of plants. In this study, parts of the needles on a ring of Pinus massoniana seedling shoots were subjected to 4 hours Dendrolimus punctatus feeding or 4 hours fumigation with 10 micromol x L(-1) of methyl jasmonate (MeJA) or terpenes, and the ABA and JA contents in treated needles, untreated neighboring sister needles, and untreated needles above and below the ring were determined by GC/MS. An obvious increase of ABA and JA contents was observed in all of the needles, whether they were treated or not, illustrating that ABA and JA were the vital signaling molecules in the wound signal transduction pathway, and participated in the formation of systematic resistance of P. massoniana seedlings.
- Published
- 2009
223. The -271 G>A polymorphism of kinase insert domain-containing receptor gene regulates its transcription level in patients with non-small cell lung cancer
- Author
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Zhi-Hong Chen, Jiaying Lin, She-Juan An, Jian Su, Yi-Long Wu, Hua-Jun Chen, and Qiu-Xiong Lin
- Subjects
Adult ,Male ,Cancer Research ,Lung Neoplasms ,Genotype ,Transcription, Genetic ,Biology ,lcsh:RC254-282 ,Young Adult ,Asian People ,Carcinoma, Non-Small-Cell Lung ,parasitic diseases ,Gene duplication ,Gene expression ,Genetics ,medicine ,Humans ,Point Mutation ,Lung cancer ,neoplasms ,Gene ,Aged ,Aged, 80 and over ,Polymorphism, Genetic ,Kinase insert domain receptor ,Middle Aged ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,medicine.disease ,Vascular Endothelial Growth Factor Receptor-2 ,Molecular biology ,Real-time polymerase chain reaction ,Oncology ,Case-Control Studies ,cardiovascular system ,Cancer research ,Female ,Gene polymorphism ,Research Article - Abstract
BackgroundKinase insert domain-containing receptor (KDR) plays a critical role in the metastasis of cancer and is used as a molecular target in cancer therapy. We investigated the characteristics of the -271 G>A polymorphism of the KDR gene to gain information that may benefit the development of individualized therapies for patients with non-small cell lung cancer (NSCLC).MethodsThe -271 G>A polymorphism of the KDR gene in 106 lung cancer patients and 203 healthy control individuals was analyzed by polymerase chain reaction (PCR) and DNA sequencing methods. Real-time quantitative PCR and immunohistochemical methods were used to evaluate KDR mRNA and protein expression levels, respectively, in frozen tumor specimens.ResultsThe -271 G>A polymorphism was associated with the mRNA expression level of the KDR gene in tumor tissues (t = 2.178, P = 0.032, independent samplest-test). Compared with the AG/GG genotype, the AA genotype was associated with higher KDR mRNA expression in tumor tissues. We found no relationship between the genotype and the KDR protein expression level and no significant difference in the distribution of the KDR gene polymorphism genotypes between lung cancer patients and the control group (χ2= 1.269, P = 0.264, Fisher's exact test).ConclusionThis study is the first to show that the -271 G>A polymorphism of the KDR gene may be a functional polymorphism related to the regulation of gene transcription. These findings may have important implications for therapies targeting KDR in patients with NSCLC.
- Published
- 2009
224. A new type of mass structured data duplicate data check.
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Wen Qi Huang, Hua Jun Chen, Xiao Bin Guo, and Li Peng
- Published
- 2016
- Full Text
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225. First-line versus second or further-line crizotinib for trial patients with advanced non-small-cell lung cancer harboring ALK rearrangements
- Author
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Xiao-Yan Bai, Yi-Sheng Huang, Wen-Zhao Zhong, Bin-Chao Wang, Hong hong Yan, Zheng Wang, Hai-Yan Tu, Qing Zhou, Jin-Ji Yang, Ben-Yuan Jiang, Xu-Chao Zhang, Yuan-Yuan Lei, Xue-Ning Yang, Jian Su, Yi-Long Wu, Hua-Jun Chen, and Chong-Rui Xu
- Subjects
Oncology ,Cancer Research ,medicine.medical_specialty ,Crizotinib ,business.industry ,First line ,medicine.disease ,respiratory tract diseases ,law.invention ,Randomized controlled trial ,law ,Internal medicine ,Medicine ,Non small cell ,Line (text file) ,business ,Lung cancer ,medicine.drug - Abstract
e19139 Background: No randomized controlled trials compared first-line to second-line crizotinib in advanced non-small-cell lung cancer (NSCLC) harboring ALK rearrangements. We aim to investigate p...
- Published
- 2015
226. Response to tyrosine kinase inhibtors in non-small-cell lung cancer with concomitant c-MET overexpression and driver genes
- Author
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Ben-Yuan Jiang, Xiao-Yan Bai, Xu-Chao Zhang, Na-Na Lou, Qing Zhou, Hua-Jun Chen, Jian Su, Zhi Xie, Yi-Long Wu, Zhi-yong Chen, Zhen Wang, and Jin-Ji Yang
- Subjects
Cancer Research ,C-Met ,business.industry ,medicine.disease ,medicine.disease_cause ,Molecular biology ,respiratory tract diseases ,chemistry.chemical_compound ,Oncology ,chemistry ,hemic and lymphatic diseases ,Concomitant ,medicine ,Cancer research ,KRAS ,Non small cell ,Lung cancer ,business ,neoplasms ,human activities ,Gene ,Tyrosine kinase - Abstract
8089 Background: The common driver genes in non-small-cell lung cancer (NSCLC) include EGFR, ALK and KRAS. We investigate the frequency of MET overexpression coexisting with oncogenic drivers and r...
- Published
- 2015
227. ROS1 rearrangement coexists with EGFR mutation in non-small-cell lung cancer
- Author
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Rui-lian Chen, Jian Su, Xu-Chao Zhang, Yue-Li Sun, Hai-Yan Tu, Jin-Ji Yang, Yi-Long Wu, Wei-Bang Guo, Qing Zhou, Na-Na Lou, Hua-Jun Chen, and Jian-Guang Chen
- Subjects
Cancer Research ,Oncogene ,biology ,Crizotinib ,ROS1 Rearrangement ,medicine.disease ,Molecular biology ,Receptor tyrosine kinase ,Oncology ,Egfr mutation ,ROS1 ,biology.protein ,medicine ,Cancer research ,Non small cell ,Lung cancer ,medicine.drug - Abstract
e19003 Background: C-ros oncogene 1 receptor tyrosine kinase (ROS1) is a newly identified gene-rearranged oncogenic receptor tyrosine kinase and patients seem to benefit from crizotinib treatment. ...
- Published
- 2015
228. Combining Diffusion Tensor Imaging and Gray Matter Volumetry to Investigate Motor Functioning in Chronic Stroke
- Author
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Huijun Li, Ya-ru Yang, Ming Yang, Gao-Jun Teng, Hua-Jun Chen, Edward H. Herskovits, Rong Chen, Yongmei Shi, Bin Liu, and Xue-song Lu
- Subjects
Adult ,Male ,medicine.medical_specialty ,Pathology ,Movement ,Caudate nucleus ,lcsh:Medicine ,Temporal lobe ,Physical medicine and rehabilitation ,Motor system ,Fractional anisotropy ,medicine ,Humans ,Gray Matter ,lcsh:Science ,Aged ,Aged, 80 and over ,Multidisciplinary ,medicine.diagnostic_test ,business.industry ,lcsh:R ,Magnetic resonance imaging ,Middle Aged ,Stroke ,Diffusion Tensor Imaging ,Corticospinal tract ,Female ,lcsh:Q ,Motor Deficit ,business ,Research Article ,Diffusion MRI - Abstract
Motor impairment after stroke is related to the integrity of the corticospinal tract (CST). However, considerable variability in motor impairment remains unexplained. To increase the accuracy in evaluating long-term motor function after ischemic stroke, we tested the hypothesis that combining diffusion tensor imaging (DTI) and gray matter (GM) volumetry can better characterize long-term motor deficit than either method alone in patients with chronic stroke. We recruited 31 patients whose Medical Research Council strength grade was ≤ 3/5 in the extensor muscles of the affected upper extremity in the acute phase. We used the Upper Extremity Fugl-Meyer (UE-FM) assessment to evaluate motor impairment, and as the primary outcome variable. We computed the fractional anisotropy ratio of the entire CST (CSTratio) and the volume of interest ratio (VOIratio), between ipsilesional and contralesional hemispheres, to explain long-term motor impairment. The results showed that CSTratio, VOIratio of motor-related brain regions, and VOIratio in the temporal lobe were correlated with UE-FM. A multiple regression model including CSTratio and VOIratio of the caudate nucleus explained 40.7% of the variability in UE-FM. The adjusted R2 of the regression model with CSTratio as an independent variable was 29.4%, and that of using VOIratio of the caudate nucleus as an independent variable was 23.1%. These results suggest that combining DTI and GM volumetry may achieve better explanation of long-term motor deficit in stroke patients, than using either measure individually. This finding may provide guidance in determining optimal neurorehabilitative interventions.
- Published
- 2015
229. [Study on fluorescence spectral behavior of trimethoxyphenylflurone with gallium in microemulsion]
- Author
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Xiao-dong, Wang, Hai-qin, Yu, Qin, Wei, Hua-jun, Chen, Bin, Du, and Xin-hua, Zhao
- Abstract
In the present paper, the fluorescence spectral behavior of gallium with trimethoxyphenylflurone (TM-PF) in microemulsion was studied. In the buffer medium of HAc-NaAc at pH 4.95, the fluorescence intensity is in proportion to the concentration of gallium (III) in the range of 0-0.045 microg x mL(-1). The CTMAB microemulsion was efficiently used to enhance the sensibility and stability of the system, and the limits of detection were 1.2 ng x mL(-1). The coexistent metal ions can be separated and gallium can be enriched by the extraction of butyl acetate, greatly improving the selectivity and sensibility of the system. The method possesses high sensitivity as well as high selectivity. It can be used to determine trace amount of gallium in ore samples successfully.
- Published
- 2006
230. [Clinical analysis of seven acute phosphine poisoning]
- Author
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Tao, Chen, Ran, Shi, Xue-zhong, Yang, Ming-jiang, Qian, and Hua-jun, Chen
- Subjects
Adult ,Male ,Occupational Diseases ,Phosphines ,Poisoning ,Acute Disease ,Humans - Published
- 2005
231. INVARIANT DISCRETIZATION OF A SYSTEM GOVERNED BY BERNOULLI'S DIFFERENTIAL EQUATION
- Author
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Hua Jun Chen, Camille Alain Rabbath, and Noriyuki Hori
- Subjects
Bernoulli differential equation ,Bernoulli's principle ,Discretization ,Differential equation ,Mathematical analysis ,Riccati equation ,Bernoulli scheme ,Bernoulli process ,Invariant (mathematics) ,Mathematics - Published
- 2004
232. Coherent optical responses and their application in biomolecule mass sensing based on a monolayer MoS_2 nanoresonator
- Author
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Hua-Jun Chen and Ka-Di Zhu
- Subjects
chemistry.chemical_classification ,Materials science ,Coupling strength ,Absorption spectroscopy ,Biomolecule ,Quantum sensor ,Physics::Optics ,Statistical and Nonlinear Physics ,Nanotechnology ,Atomic and Molecular Physics, and Optics ,Nanomaterials ,Nonlinear optical ,chemistry ,Monolayer ,Visible spectrum - Abstract
Graphene-like nanomaterials such as monolayer MoS2 have attracted considerable attention recently. In this article, we propose a nano-optomechanical system based on a suspended monolayer MoS2. The coherent linear and nonlinear optical properties of this system have been investigated theoretically. A scheme for measuring the coupling strength of exciton–nanoresonator and the frequency of the nanoresonator is also demonstrated by the optical pump-probe method. Based on the coherent optical spectrum, we further propose a nonlinear optical scheme to determine the mass of the biomolecules (such as single influenza virus and HIV virus). This scheme proposed here may have potential applications in quantum sensing and all-optical MoS2-based devices.
- Published
- 2014
233. Simultaneous detection of multiple key genes in Chinese non-small cell lung cancer was feasible in clinical practice
- Author
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Xu-Chao Zhang, Zhi-Hong Chen, Jian Su, Jin-Ji Yang, Yi-Long Wu, Hong-Hong Yan, and Hua-Jun Chen
- Subjects
Clinical Practice ,Cancer Research ,Oncology ,Key genes ,business.industry ,medicine ,Non small cell ,Lung cancer ,medicine.disease ,Bioinformatics ,business ,respiratory tract diseases - Abstract
e19149 Background: The identification of genetic mutational profiles is significant in personalized management of non-small cell lung cancer (NSCLC). Previous techniques are limited to analyzing on...
- Published
- 2014
234. A phase II trial of first-line nab-paclitaxel/carboplatin versus gemcitabine/carboplatin in advanced squamous cell carcinoma of the lung (CTONG1002)
- Author
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Hua-Jun Chen, Xiao-song Ben, Yi-Long Wu, Cheng Huang, Qing Zhou, Yong Song, Hong-Hong Yan, Ying Cheng, Jin-Ji Yang, and Gongyan Chen
- Subjects
Oncology ,Cancer Research ,medicine.medical_specialty ,Squamous-cell carcinoma of the lung ,business.industry ,First line ,medicine.disease ,Gemcitabine ,Carboplatin ,chemistry.chemical_compound ,chemistry ,Internal medicine ,Medicine ,In patient ,business ,Lung cancer ,medicine.drug ,Nab-paclitaxel - Abstract
8085 Background: The administration of nab-paxlitaxel/carboplatin (nab-PC) as first-line therapy in patients with advanced non-small-cell lung cancer was efficacious and resulted in a significantly...
- Published
- 2014
235. Overall survival in patients with advanced non-small cell lung cancer harboring concomitant EGFR mutations and ALK rearrangements: A cohort study
- Author
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Hai-Yan Tu, Hong-Hong Yan, Xu-Chao Zhang, Xiao-Yan Bai, Qing Zhou, Ben-Yuan Jiang, Hua-Jun Chen, Jian Su, Yi-Sheng Huang, Bin-Chao Wang, Chong-Rui Xu, Yi-Long Wu, Zhen Wang, and Jin-Ji Yang
- Subjects
Cancer Research ,Pathology ,medicine.medical_specialty ,integumentary system ,biology ,business.industry ,medicine.disease ,Oncology ,Egfr mutation ,hemic and lymphatic diseases ,Concomitant ,Cancer research ,biology.protein ,Medicine ,Anaplastic lymphoma kinase ,In patient ,Epidermal growth factor receptor ,Non small cell ,business ,Lung cancer ,Cohort study - Abstract
e19010 Background: Concomitant epidermal growth factor receptor (EGFR) mutations and anaplastic lymphoma kinase (ALK) rearrangements define a rare molecular subtype of non-small-cell lung cancer (N...
- Published
- 2014
236. MET overexpression as a promising therapeutic target in non-small cell lung cancer with acquired resistance to EGFR TKIs
- Author
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Xu-Chao Zhang, Zhen Wang, Bin-Chao Wang, Hua-Jun Chen, Jian Su, Zhi Xie, Xiao-Yan Bai, Chong-Rui Xu, Qing Zhou, Jin-Ji Yang, Yi-Long Wu, Yi-Sheng Huang, Hai-Yan Tu, and Ben-Yuan Jiang
- Subjects
Oncology ,Cancer Research ,medicine.medical_specialty ,business.industry ,medicine.disease_cause ,medicine.disease ,respiratory tract diseases ,Clinical trial ,Gefitinib ,Internal medicine ,ROS1 ,medicine ,Immunohistochemistry ,KRAS ,Erlotinib ,Lung cancer ,business ,MET Positive ,neoplasms ,medicine.drug - Abstract
e19047 Background: MET amplification is recognized as one of common molecular mechanisms of acquired resistance (AR) to epidermal growth factor receptor tyrosine kinase inhibitors (EGFR TKIs) in advanced non-small-cell lung cancer (NSCLC). The aim of the present study is to investigate the frequency of MET overexpression, the correlated clinicopathologic features and the possible treatments in NSCLC with AR. Methods: Advanced NSCLC patients with AR to EGFR TKIs were detected for MET overexpression by immunohistochemistry (IHC). ≥50% tumor cells with moderate to high intensity staining were defined as MET positive. MET positive patients were recommended to participate a clinical trial if eligible or receive other molecularly targeted therapies. The statuses of EGFR, ALK, KRAS and ROS1 were also tested. Results: From January 2013 to January 2014, 58 advanced NSCLC patients with AR to gefitinib or erlotinib were enrolled prospectively. The frequencies of MET overexpression was 32.8% (19/58), MET overexpressi...
- Published
- 2014
237. Ultrasensitive mass sensing with nonlinear optics in a doubly clamped suspended carbon nanotube resonator
- Author
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Hua-Jun Chen and Ka-Di Zhu
- Subjects
Nanostructure ,business.industry ,General Physics and Astronomy ,chemistry.chemical_element ,Nonlinear optics ,Nanotechnology ,Carbon nanotube ,law.invention ,Resonator ,chemistry ,Nanosensor ,law ,Molecular vibration ,Optoelectronics ,business ,Spectroscopy ,Carbon - Abstract
Nanomechanical resonator makes itself as an ideal system for ultrasensitive mass sensing due to its ultralow mass and high vibrational frequency. The mass sensing principle is due to the linear relationship of the frequency-shift and mass-variation. In this work, we will propose a nonlinear optical mass sensor based on a doubly clamped suspended carbon nanotube resonator in all-optical domain. The masses of external particles (such as nitric oxide molecules) landing onto the surface of carbon nanotube can be determined directly and accurately via using the nonlinear optical spectroscopy. This mass sensing proposed here may provide a nonlinear optical measurement technique in quantum measurements and environmental science.
- Published
- 2013
238. Anaplastic Lymphoma Kinase Variants and the Percentage of ALK-Positive Tumor Cells and the Efficacy of Crizotinib in Advanced NSCLC.
- Author
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Yuan-Yuan Lei, Jin-Ji Yang, Xu-Chao Zhang, Wen-Zhao Zhong, Qing Zhou, Hai-Yan Tu, Hong-Xia Tian, Wei-Bang Guo, Lu-Lu Yang, Hong-Hong Yan, Hua-Jun Chen, Zhi Xie, Jian Su, Jie-Fei Han, Yi-Long Wu, Lei, Yuan-Yuan, Yang, Jin-Ji, Zhang, Xu-Chao, Zhong, Wen-Zhao, and Zhou, Qing
- Published
- 2016
- Full Text
- View/download PDF
239. Distribution and prognosis of uncommon metastases from non-small cell lung cancer.
- Author
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Fei-Yu Niu, Qing Zhou, Jin-Ji Yang, Wen-Zhao Zhong, Zhi-Hong Chen, Wei Deng, Yan-Yan He, Hua-Jun Chen, Zhu Zeng, Ke, E. -E., Ning Zhao, Na Zhang, Hui-Wen Sun, Qiu-Yi Zhang, Zhi Xie, Xu-Chao Zhang, Yi-Long Wu, Niu, Fei-Yu, Zhou, Qing, and Yang, Jin-Ji
- Subjects
NON-small-cell lung carcinoma ,CANCER treatment ,CANCER invasiveness ,HEALTH outcome assessment ,DIAGNOSIS ,PROGNOSIS ,LUNG cancer ,LUNG tumors ,METASTASIS ,SURVIVAL ,PROPORTIONAL hazards models - Abstract
Background: According to the literature and our experience, the most common sites of non-small cell lung cancer (NSCLC) metastases include the brain, bone, liver, adrenal glands, contralateral lung and distant lymph nodes. Metastases to other organs are relatively rare. There have been numerous case reports and a few small case series of uncommon metastases derived from NSCLC.Methods: We defined all organs except the common metastatic sites mentioned above as uncommon sites of metastasis. Patients with uncommon metastases among 2,872 consecutive NSCLC patients with stage IV disease at the Guangdong Lung Cancer Institute (GLCI) from 2006 to 2012 were included in this study. The diagnosis of uncommon metastases was based on pathology or imaging studies.Results: Uncommon metastases were diagnosed in 193 cases at anatomical sites such as the soft tissue, kidney, pancreas, spleen, peritoneum, intestine, bone marrow, eye, ovary, thyroid, heart, breast, tonsil and nasal cavity. Uncommon metastases were identified as independent poor prognostic factors through a multivariate analysis with a HR (hazard ratio) of 1.29 [95% confidence interval (CI) 1.09-1.52, P < 0.01]. Those patients who received systemic therapy plus local treatment had a better survival rate than did those who received systemic therapy only (P < 0.01); all patients received best supportive care.Conclusions: Metastases to the above mentioned sites are infrequent. The presentation of uncommon metastases tends to indicate a poor outcome, and selected patients may benefit from local treatment. [ABSTRACT FROM AUTHOR]- Published
- 2016
- Full Text
- View/download PDF
240. Single nucleotide polymorphisms in VTI1A gene contribute to the susceptibility of Chinese population to non-small cell lung cancer.
- Author
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Wen-Mei Su, Zhi-Hong Chen, Xu-Chao Zhang, Jian Su, Zhi Xie, Hong-Hong Yan, Jin-Ji Yang, Hua-Jun Chen, Qing Zhou, Wen-Zhao Zhong, Wei-Bang Guo, Shi-Liang Chen, and Yi-Long Wu
- Published
- 2015
- Full Text
- View/download PDF
241. Nonlinear optical response of cavity optomechanical system with second-order coupling.
- Author
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CHONG WANG, HUA-JUN CHEN, and KA-DI ZHU
- Published
- 2015
- Full Text
- View/download PDF
242. Using geodata and geoprocessing web services in embedded device.
- Author
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Deng-hui Zhang, Bin Xie, Hua-jun Chen, Yang Lv, and Le Yu
- Published
- 2010
- Full Text
- View/download PDF
243. Effect of the Chinese herbal extract on androgen-insensitive prostate cancer cell lines in vitro and in mice
- Author
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Wen Guo Jiang, Guoqiang Wang, Yuqin Liu, J. M. Guo, Zhiguo Zhang, Yang Zhang, G. Du, and Hua-Jun Chen
- Subjects
Cancer Research ,Pathology ,medicine.medical_specialty ,medicine.diagnostic_test ,business.industry ,medicine.drug_class ,Cell cycle ,medicine.disease ,Androgen ,Flow cytometry ,Prostate cancer ,Oncology ,Western blot ,Apoptosis ,Toxicity ,Cancer cell ,medicine ,Cancer research ,business - Abstract
4741 Background: An herbal combination PC-SPES was available in US for prostate cancer treatment and withdrawn in 2002 due to contamination. In China, the number of Prostate cancer recurrence after surgical castration has increased recently. There is an urgent need to find an effective therapy for these patients. We therefore investigated the effect of the seven Chinese herbal extract in PC- SPES on androgen insensitive prostate cancer (AIPC) cell lines and in nude mice inoculated with the cancer cells. Methods: LC-MS was used to detect any synthetic drug contamination. Flow cytometry was used to measure cell cycle and apoptosis. Western blot was used to measure p16, p-RB, p21, Bax, Bad, bcl-2 and Bcl-X, Caspase and PARP protein expressions. IL-6 was detected by the method of ELISA. Each group of 40 nude mice was inoculated with PC-3 and DU-145 separately. Oral administration of the herbal extract continued for 8 weeks. After sacrifice, various tissues were examined for pathological toxicity and the tumor...
- Published
- 2005
244. INVARIANT DISCRETIZATION OF A SYSTEM GOVERNED BY BERNOULLI'S DIFFERENTIAL EQUATION.
- Author
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NORIYUKI HORI, RABBATH, CAMILLE ALAIN, and HUA JUN CHEN
- Subjects
BERNOULLI equation ,DIFFERENTIAL equations ,SYSTEMS theory ,INVARIANTS (Mathematics) ,DISCRETE systems - Published
- 2004
245. Coherent optical responses and their application in biomolecule mass sensing based on a monolayer MoS2 nanoresonator.
- Author
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Hua-Jun Chen and Ka-Di Zhu
- Published
- 2014
- Full Text
- View/download PDF
246. EGFR Mutation Heterogeneity and the Mixed Response to EGFR Tyrosine Kinase Inhibitors of Lung Adenocarcinomas.
- Author
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ZHI-YONG CHEN, WEN-ZHAO ZHONG, XU-CHAO ZHANG, JIAN SU, XUE-NING YANG, ZHI-HONG CHEN, JIN-JI YANG, QING ZHOU, HONG-HONG YAN, SHE-JUAN AN, HUA-JUN CHEN, BEN-YUAN JIANG, MOK, TONY S., and YI-LONG WU
- Subjects
LUNG tumors ,CONFIDENCE intervals ,ENZYME inhibitors ,MULTIVARIATE analysis ,GENETIC mutation ,DATA analysis software ,DESCRIPTIVE statistics ,SEQUENCE analysis ,GENETICS - Abstract
Background. Non-small cell lung cancer patients with epidermal growth factor receptor (EGFR) mutations have mixed responses to tyrosine kinase inhibitors (TKIs). Intertumor heterogeneity in EGFR mutations is one potential explanation for this phenomenon. Methods. We performed direct sequencing to identify EGFR mutations in 180 pairs of lung adenocarcinoma samples (from 3,071 patients). The high-resolution melting method was used in discordant cases to confirm EGFR mutation status. Matching samples were divided into four groups: primary lesions detected at different times, primary tumors with matched metastatic lymph nodes, multiple pulmonary nodules, and primary tumors with matched distant metastases. Multivariate analyses were performed to evaluate correlations between heterogeneity and patient characteristics. Results. In the study population, the discordance rate was 13.9% (25 of 180). The multiple pulmonary nodules group had the highest discordance rate of 24.4% (10 of 41; odds ratio for heterogeneity in primary lesions detected at different times, 6.37; 95% confidence interval, 1.71-23.72; p = .006). Discordance rates in the metachronous and synchronous settings were 15.7% (22 of 140) and 7.5% (three of 40), respectively. In the 34 patients who developed EGFR TKI resistance, 10 (29.4%) cases exhibited heterogeneity and five (14.7%) patients exhibited a mixed response to the drug. Three (8.8%) of the patients with a mixed response also exhibited discordant EGFR mutations. Conclusions. The overall discordance rate of EGFR mutation heterogeneity in Asian patients with pulmonary adenocarcinoma is relatively low, but the rate in patients with multiple pulmonary nodules is significantly higher. This observation may explain the mixed tumor response to EGFR TKIs. [ABSTRACT FROM AUTHOR]
- Published
- 2012
- Full Text
- View/download PDF
247. RCCtrust: A Combined Trust Model for Electronic Community.
- Author
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Yu Zhang, Hua-Jun Chen, Xiao-Hong Jiang, Hao Sheng, and Zhao-Hui Wu
- Subjects
COMPUTER systems ,MATHEMATICAL models ,RECOMMENDER systems ,RATING ,COMPUTER users ,PROBLEM solving - Abstract
Abstract Previous trust models are mainly focused on reputational mechanism based on explicit trust ratings. However, the large amount of user-generated content and community context published on Web is often ignored. Without enough information, there are several problems with previous trust models: first, they cannot determine in which field one user trusts in another, so many models assume that trust exists in all fields. Second some models are not able to delineate the variation of trust scales, therefore they regard each user trusts all his friends to the same extent. Third, since these models only focus on explicit trust ratings, so the trust matrix is very sparse. To solve these problems, we present RCCtrust – a trust model which combines Reputation-, Content- and Context-based mechanisms to provide more accurate, fine-grained and efficient trust management for the electronic community. We extract trust-related information from user-generated content and community context from Web to extend reputation-based trust models. We introduce role-based and behavior-based reasoning functionalities to infer users' interests and category-specific trust relationships. Following the study in sociology, RCCtrust exploits similarities between pairs of users to depict differentiated trust scales. The experimental results show that RCCtrust outperforms pure user similarity method and linear decay trust-aware technique in both accuracy and coverage for a Recommender System. [ABSTRACT FROM AUTHOR]
- Published
- 2009
- Full Text
- View/download PDF
248. Dynamic Query Optimization Approach for Semantic Database Grid.
- Author
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Xiao-Qing Zheng, Hua-Jun Chen, Zhao-Hui Wu, and Yu-Xin Mao
- Abstract
Abstract Fundamentally, semantic grid database is about bringing globally distributed databases together in order to coordinate resource sharing and problem solving in which information is given well-defined meaning, and DartGrid II is the implemented database gird system whose goal is to provide a semantic solution for integrating database resources on the Web. Although many algorithms have been proposed for optimizing query-processing in order to minimize costs and/or response time, associated with obtaining the answer to query in a distributed database system, database grid query optimization problem is fundamentally different from traditional distributed query optimization. These differences are shown to be the consequences of autonomy and heterogeneity of database nodes in database grid. Therefore, more challenges have arisen for query optimization in database grid than traditional distributed database. Following this observation, the design of a query optimizer in DartGrid II is presented, and a heuristic, dynamic and parallel query optimization approach to processing query in database grid is proposed. A set of semantic tools supporting relational database integration and semantic-based information browsing has also been implemented to realize the above vision. [ABSTRACT FROM AUTHOR]
- Published
- 2006
249. Volatile Organic Compounds and Their Roles in Bacteriostasis in Five Conifer Species.
- Author
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Yan Gao, You-Ju Jin, Hai-Dong Li, and Hua-Jun Chen
- Subjects
VOLATILE organic compounds ,ORGANIC compounds ,PHYTOCHEMICALS ,MICROBIAL growth ,CONIFERS ,CHROMATOGRAPHIC analysis ,BOTANICAL chemistry - Abstract
In order to make clear the functions of plant volatile organic compounds (VOCs) on bacteriostasis and air decontamination, we analyzed the composition and content of VOCs inPinus tabulaeformisCarr.,P. bungeanaZucc.,Sabina chinensisAntoine,Picea koraiensisNakai, andCedrus deodaraG. Don under near-natural conditions using the thermal-desorption cold trap gas chromatography/mass spectrometer technique. The effects of the VOCs on airborne microorganisms were investigated using the method of natural sedimentation. Results showed that the major VOCs were as follows: limonene,β-pinene,α-pinene, andα-caryophyllene inPinus tabulaeformisandP. bungeana; limonene, borneol acetate,β-pinene, myrcene, and tricylene inS. chinensis; limonene,α-pinene, myrcene, camphene, andβ-pinene inPicea koraiensis; and limonene, 2, (10)-pinene,α-pinene, and myrcene inC. deodara. These VOCs and the corresponding foliar extracts inhibited the growth of bacteria and stimulated the growth of fungi. Experimental data using monomers of the VOCs demonstrated that limonene,β-pinene, and three aldehydes could significantly inhibit bacterial growth, suggesting an inhibitory effect of VOCs on the growth of airborne microorganisms in the five conifer species. The bacteriostasis and air-decontaminating effects of plant VOCs are further discussed in terms of their chemical composition.(Managing editor: Wei WANG) [ABSTRACT FROM AUTHOR]
- Published
- 2005
- Full Text
- View/download PDF
250. Robust tuning of high-Q spoof plasmonic cavity with surface defect.
- Author
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Hong-Wei Wu, Hua-Jun Chen, Ren-Hao Fan, Yang Li, and Zong-Qiang Sheng
- Abstract
We demonstrate that tunable whispering gallery modes can be supported in a spoof plasmonic cavity with a deep-subwavelength scale by inserting periodic metal strips into a silicon cylinder. The resonant frequencies of modes can be tuned freely by properly tailoring the structural parameters. Furthermore, we also show that the resonant modes can keep a high quality factor for a defect existing on the surface of the spoof plasmonic cavity by switching the whispering gallery resonance to Fabry–Perot-like resonance, which is absent for the traditional whispering gallery cavities. This property indicates potential applications in the design of optical cavities at low frequencies. [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
- View/download PDF
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