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BackgroundAlthough numerous mouse models of breast carcinomas have been developed, we do not know the extent to which any faithfully represent clinically significant human phenotypes. To address this need, we characterized mammary tumor gene expression profiles from 13 different murine models using DNA microarrays and compared the resulting data to those from human breast tumors.ResultsUnsupervised hierarchical clustering analysis showed that six models (TgWAP-Myc, TgMMTV-Neu, TgMMTV-PyMT, TgWAP-Int3, TgWAP-Tag, and TgC3(1)-Tag) yielded tumors with distinctive and homogeneous expression patterns within each strain. However, in each of four other models (TgWAP-T121, TgMMTV-Wnt1, Brca1Co/Co;TgMMTV-Cre;p53+/- and DMBA-induced), tumors with a variety of histologies and expression profiles developed. In many models, similarities to human breast tumors were recognized, including proliferation and human breast tumor subtype signatures. Significantly, tumors of several models displayed characteristics of human basal-like breast tumors, including two models with induced Brca1 deficiencies. Tumors of other murine models shared features and trended towards significance of gene enrichment with human luminal tumors; however, these murine tumors lacked expression of estrogen receptor (ER) and ER-regulated genes. TgMMTV-Neu tumors did not have a significant gene overlap with the human HER2+/ER- subtype and were more similar to human luminal tumors.ConclusionMany of the defining characteristics of human subtypes were conserved among the mouse models. Although no single mouse model recapitulated all the expression features of a given human subtype, these shared expression features provide a common framework for an improved integration of murine mammary tumor models with human breast tumors.

Tiltrotors permit aircrafts to operate vertically with lift, yet convert to ordinary forward flight with thrust. The challenge is to design a tiltrotor blade yielding maximum lift and thrust that converts smoothly without losing integrity or efficiency. The two types of blades, soft in-plane and stiff in-plane—the designation depending on the value of the blade's natural lag frequency—exhibit different structural responses under the same flight conditions, differently affecting the aerodynamics of the blades, especially in the complex aerodynamic environment of conversion flight where the aerodynamic differences are significant. This phase of flight is not deeply researched, nor is the analytical coupling method much used. To study the influence of blade type on aerodynamics during conversion, models suitable for the conversion flight simulation are established for the application of coupled computational fluid dynamics and computational structural dynamics (CFD-CSD) methods. Each method is implemented with well-accepted techniques (the Unsteady Reynolds-Averaged Navier–Stokes (URANS) equations, the Reverse Overset Assembly Technique (ROAT), and the Timoshenko beam model. To improve the solving efficiency, a loose coupling strategy is used in constructing the two-way coupled model. The XV-15 tiltrotor is used for verification. The aeroelastic simulation of soft in-plane and stiff in-plane blades in conversion flight indicates an impactful role on the modal shapes, with a significant difference in the third flap modal shapes for the XV-15 rotor. However, the effect on aerodynamic performance is relatively small. In the first half of the flight conversion, the thrust of stiff in-plane blades is larger than that of soft in-plane blades, but in the last half, the influence of structural characteristics on aerodynamic performance is negligible and the thrust of the blades tends to be equal. [ABSTRACT FROM AUTHOR]

This study develops a hybrid solver with reversed overset assembly technology (ROAT), a viscous vortex particle method (VVPM), and a CFD program based on the URNS method, in order to study the aerodynamic and acoustic characteristics of coaxial rigid rotors. The aerodynamic load of the "AH-1G" helicopter rotor is first calculated based on the hybrid method and compared with available experimental data. The prediction of the linear noise of the OLS rotor is then performed and the obtained results are compared with available experimental data. These results allow the evaluation of the accuracy of the hybrid method for emulating rotor aerodynamics and acoustics. Afterwards, the hybrid and CFD methods are applied to obtain the aerodynamic and acoustic characteristics of the given coaxial rigid rotor model, while taking into account the trim of the collective pitch. The obtained results demonstrate that the hybrid method has high proficiency in capturing blade–vortex-interaction impulsive loads and high computational efficiency in predicting associated loading noise characteristics. Furthermore, the effect of the hybrid method on the noise characteristics of coaxial rigid rotors under a different advance ratio, blade tip speed, shaft angle, and other conditions, as well as the impact of the upper and lower rotors on the noise contribution of the coaxial rotor are analyzed. Finally, the impacts of the initial phase and the vertical spacing on the sound pressure level are studied. [ABSTRACT FROM AUTHOR]

In order to investigate the influence of freshwater fish ponds on water and salt transport in cultivated wasteland in salinized areas, a typical study area was selected in the middle and lower reaches of the Hetao Irrigation District in China in the Yichang Irrigation Domain, and the temporal and spatial changes in the salinity of soil and salinity of groundwater and fish pond water in the cultivated–wasteland–fish pond system were characterized through the monitoring of the environmental information of soil and groundwater at the boundaries of the cultivated land, wasteland, and fish ponds. Salinity changes and groundwater migration in different periods were determined, and the response of soil salinity to the depth of groundwater burial was analyzed, as well as the effect of fish ponds on soil salinization. The results showed that the amount of groundwater migrating from cropland to wasteland during the simulation period in 2022 was 2700 m3, the amount of groundwater migrating from wasteland to fish ponds was 630 m3, and the amount of groundwater migrating from fish ponds to wasteland during the fall watering period was 440 m3. From an overall perspective, the average soil salinity of wasteland was 1.56 times higher than that of the boundary of fish ponds. Not only do fish ponds play a positive role in the ecosystem, but they also have a desalinization effect that reduces soil salinity significantly. Groundwater depth and soil salinity have an exponential relationship; when the depth of groundwater is greater than 1.75 m, the soil salinity varies little with the depth of groundwater and the soil salinity is less than 0.66 ds/m, which can be determined as the critical depth, and the average depth of groundwater in cultivated land in the study area is 1.5 m. Therefore, it is necessary to reasonably control the water level of fish ponds, so as to make the groundwater depth of cultivated land control between 1.75 m and 2.0 m, and to prevent soil salinization. [ABSTRACT FROM AUTHOR]

Research on helicopter transient maneuvering flight noise is a hotspot and challenging topic in the fields of helicopter design and application. A new time-accurate free-wake (TAFW) method and the Fowcs Williams-Hawkings (FW–H) equations are applied to analyze the aerodynamic and noise responses of a rotor subjected to a ramp increase in collective pitch, in hover, and in forward flight. First, a TAFW algorithm suitable for rotor aerodynamic simulation in steady-state flight and transient maneuvers is developed using modified third-order upwind backward differentiation formulas. Then, to verify the effectiveness and accuracy of the proposed method, various parameters are calculated for two scenarios and compared with corresponding results from experiments by the University of Maryland: the Langley 2MRTS rotor and the NACA rotor with ramp increases in collective pitch. Finally, the influence of collective pitch increase rate, the total increase of collective pitch, and the start and stop azimuth of ramp increase on the aerodynamic and loading noise responses of the rotor are analyzed in hover and forward flight conditions. The results show the ramp increase in collective pitch will affect the loading noise in three timescales: short-term, medium-term, and long-term. The change of the loading noise is greater when the collective pitch increase rate is greater, and the start and stop azimuth angles of the ramp increase are also important factors affecting the aerodynamic load distribution and directionality of the noise. [ABSTRACT FROM AUTHOR]