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Using homology cloning method, a heat shock transcription factor (Hsf) like gene, ZmHsf-like , was cloned from maize ( Zea mays ) leaves. Sequence analyses showed that the open reading frame (ORF) of the gene ZmHsf-like is 1 404 bp long, encoding 467 amino acids. The sequence of amino acids encoded by ZmHsf-like contains the most conserved and typical DNA-binding domain of Hsf family. By bombardment into onion epidermis, we firstly found that the ZmHsf-like was subcellular-located in nucleus. NucPred analysis revealed there is a classic NLS of KKRR peptide in protein. Real-time PCR showed that ZmHsf-like gene expressed in leaves, stems and roots of maize seedlings under normal growth conditions, and the highest expression level was in roots, lower in leaves and the lowest in stems. The ZmHsf-like gene expression could be up-regulated by heat shock, PEG, ABA, and H 2 O 2 in different degrees, among which the heat shock and ABA worked more efficiently. Obvious differences of the peak value and its corresponding time point of ZmHsf-like gene expression were observed among treatments. Experiments with inhibitor further suggested that the up-regulation ZmHsf-like gene expression of heat shock was H 2 O 2 -dependent while the induction of ZmHsf-like with PEG did not depend on the existence of H 2 O 2 . These results pointed out that ZmHsf-like gene probably regulates responsive reactions to abiotic stresses especially heat shock and drought through different signal transduction pathways.

The interaction between a hydrophobic-functionalized PAMAM dendrimer (PAMAM-NH2-C12, 25%, G4) and bovine serum albumin (BSA) has been investigated by circular dichroism (CD), UV-vis, and fluorescence spectroscopic methods and molecular modeling. The analysis of the effects of dendrimer complexation on the stability and conformation of BSA indicated that the binding process of the hydrophobic-functionalized dendrimer with BSA induced the relatively large changes in secondary structure of protein. Thermal denaturation of BSA, when carried out in the presence of dendrimer, also indicated that this hydrophobic-functionalized dendrimer acted as a structure destabilizer for BSA. The hydrophobic, electrostatic, and hydrogen bonding forces played important roles in the complex formation. The putative binding site of PAMAM-NH2-C12 (25%) dendrimer on BSA was near to domain I and domain II. The effect of hydrophobic modification on the stability and structure of BSA would find useful information on the cytotoxicity of PAMAM dendrimer.

Thyroid hormone (TH) is essential for the proper development of mammalian central nervous system. TH deficiency during the critical period of brain development results in permanent cognitive and neurological impairments. Members of the protein kinase C (PKC) family play a key role in the regulation of cellular functions in the nervous system. Alteration of PKC can be involved in the pathogenesis of neuronal disorders. This review details recent progress made in determining the roles played by PKC isoforms in developing hypothyroid rat brain. Evidence indicates that hippocampus down-regulation of PKCβ and PKCγ may be related to impaired learning and memory observed in perinatal hypothyroid rats. Enhanced PKCα activity in neonatal hypothyroid brain may bring about oxidative stress and cause brain damage. The activated pro-apoptotic PKCs including PKCδ can cause extensive apoptosis in the hypothyroid rat brain.

Ten new coordination complexes, [Cd3(H2L)(H2O)7]·6H2O (1), [Mn3(H2L)(H2O)7]·7H2O (2), [Zn3(H2L)(H2O)6]·6H2O (3), [Co3(H2L)(H2O)6]·5.75H2O (4), [Fe3(H2L)(H2O)8]·6.5H2O (5), [Co3(H2L)(teta)3]·5H2O (6), [Ni4(L)(teta)4]·2.5H2O (7), [Ni3(H2L)(teta)(H2O)6]·6H2O (8), [Cd4(L)(teta)4(H2O)2]·H2O (9), and [Cu2(H4L)(teta)2]·2H2O (10) (H8L = 1,4,8,11-tetrazacyclododecane-N,N′,N″,N‴-tetra-methylene-isophthalic acid and teta = 5,5,7,12,12,14-hexamethyl-1,4,8,11-tetraazacyclotetradecane) have been synthesized. Their structures have been confirmed and further characterized by relative physical methods. Compounds 1 and 2 are isomorphous and demonstrate the identical three-dimensional (3D) (4,6)-connected nets with (64·72)(68·76·8) topologies. Compounds 3–5 are isostructural and unfold 3D 4-fold interpenetrating frameworks with (412·63) topologies. Compound 6 is a 3D 2-fold interpenetrating framework with (412·63) topology. Compound 7 features a 3D 6-connected (412·63) net. Compounds 8 and 9 reveal two-dimensional (2D) laye...

Four metal–organic frameworks (MOFs), namely [Cu4(L)2(μ3-OH)2(CH3OH)2(H2O)] (1), [Co2(L)(μ3-OH)(H2O)2] (2), [Cd2(L)(μ3-OH)(H2O)2] (3) and [Cd9(L)4(μ3-OH)4(CH3O)2(H2O)2]·(H2O)·2(CH3OH) (4) where H3L = (3,5-dicarboxyl-phenyl)-(4-(2′-carboxyl-phenyl)-benzyl) ether, have been prepared. Compound 1 displays a (3,6)-connected net with kgd (kagome dual) topology based on discrete {Cu4} secondary building blocks. Compounds 2 and 3 possess similar two-dimensional (2D) structures, which are constructed by L anions and rod-shaped SBUs. In compound 4, neighboring {Cd9} units are bridged by carboxylate groups to build a rod-shaped SBU. These SBUs are further extended by L anions into a 3D framework. The optical band gaps of 1 and 2 and the photoluminescent behaviors of 3 and 4 have been investigated. In addition, the photocatalytic activities of compounds 1 and 2 have been estimated under UV irradiation.

Cordyceps militaris has similar chemical composition, nutrition value, and medicinal functions as Cordyceps sinensis. However, its main medical composition such as cordycepin is much higher than that of the wild C. sinensis species. In addition, it is easier to cultivate artificially than C. sinensis. Thus, C. militaris is chosen as the best substitution for C. sinensis. This paper introduces the pharmacological effects of C. militaris and the effect of selenium on human body. It also summarizes ways of improving the content of selenium in artificially cultivated C. militaris and the optimal conditions for its cultivation. Low concentration of selenium in the medium can stimulate the growth of C. militaris mycelium. However, selenium of high concentration in the medium prevents its growth and decreases the conversion efficiency from inorganic to organic selenium.

A new composite strengthening method that the CFST short column was strengthened with concrete filled steel tube was presented. Through the finite element analysis of five specimens with strengthening circular concrete filled steel tube columns and a specimen without strengthening circular concrete filled steel tube to explore the impact of the outer layer of concrete strength grade, external pipe wall thickness for the ultimate bearing capacity of concrete filled steel tube columns. The results show that with the increase of the outer pipe wall thickness, double concrete filled steel tube column yield strength and ultimate strength have increased. As the outer concrete strength grade increased as the specimen bearing capacity increased. When the concrete strength grade greater than C40, the improvement of concrete strength for specimen ultimate bearing capacity is not great.

Hybrid millet Zhang and its parental cultivars were studied for their potassium (K) uptake and accumulation characteristics and related physiological mechanisms. Hydroponic culture was performed with two K levels (i.e., high and low) set up. At high K level, hybrid millet showed heterobeltiosis in K accumulation and leaf K content, and it also had higher H+-ATPase activity, respiration rate, root oxidant activity and root K+ influx rate than its parental cultivars. All these lay the physiological foundation of heterosis for potassium uptake of hybrid millet. At low K level, the hybrid millet had a lower H+-ATPase activity and a higher K efflux rate than its parental cultivars, thus heterobeltiosis in K accumulation or leaf K content was not observed. Therefore, high level K fertilizer application is recommended for hybrid millet cultivation as it is favorable for hybrid millet to display heterosis in K uptake and K accumulation.

In the present study, we investigated oxidative stress parameters and antioxidant defence status in perinatal hypothyroid rat liver and heart. We found that the proteincarbonyl content did not differ significantly between the three groups both in the pup liver and in the heart. The OH˙ level was significantly decreased in the hypothyroid heart but not in the liver compared with controls. A slight but not significant decrease in SOD activity was observed in both perinatal hypothyroid liver and heart. A significantly increased activity of CAT was observed in the liver but not in the heart of hypothyroid pups. The GPx activity was considerably increased compared with controls in the perinatal hypothyroid heart and was unaltered in the liver of hypothyroid pups. We also found that vitamin E levels in the liver decreased significantly in hypothyroidism and were unaltered in the heart of perinatal hypothyroid rats. The GSH content was elevated significantly in both hypothyroid liver and heart. The total antioxidant capacity was higher in the liver of the hypothyroid group but not in the hypothyroid heart. Thyroxine replacement could not repair the above changes to normal. In conclusion, perinatal hypothyroidism modulates the oxidative stress status of the perinatal liver and heart.

Potassium (K) is a key component of plant nutrition, significantly influencing crop growth. Levels of this nutrient in plants can also influence a number of pest infestations. The brown planthopper, Nilaparvata lugens (Stal) (Hemiptera: Delphacidae), is an important pest of rice in Asia. In this study, we examined K contents in rice grown in hydroponic solution, and its relationship to the fecundity and expression of the vitellogenin (Nlvg) gene of N. lugens which was reared on the rice. Our findings indicated that K contents in rice increased with the increasing K concentration within the hydroponic solution, but reduced at the highest K concentration (160 mg/L). The number of eggs laid by N. lugens which was reared on the rice varied significantly with K concentration, and increased by 0.12 and 0.22 fold under 20 mg/L and 160 mg/L K level compared to that of the control (40 mg/L), decreasing by 0.57 fold under 0 mg/L K. Nlvg mRNA expression increased 1.17 and 1.94 fold in individuals which were reared on rice grown in 20 mg/L and 160 mg/L K level, compared to that of the control before mating; and by 3.36 and 2.97 after mating, respectively. However, Nlvg mRNA expression fold decreased by 0.99 under 0 mg/L K level before mating and 0.91 after mating. The variation of eggs may be attributed to the change of Nlvg mRNA expression, because there was a positive correlation between the eggs and Nlvg mRNA expression. These results demonstrated low (20 mg/L) and highest K levels (160 mg/L) in hydroponic solution showed the lower K level in plants than the control, which facilitated the fecundity of N. lugens. The study of the effects of K levels on the fecundity should have significance for insect control.

Liposarcoma has previously been described in Western studies, however, such cases are rarely reported in the mediastinum. In addition, the presence of a liposarcoma with smooth muscle and neural differentiation has not been previously reported. Thus, the present study describes the rare case of a 28-year-old Chinese male admitted to our hospital with the symptoms of chest tightness and shortness of breath due to a recurrent fibrolipoma in the mediastinum. The resected tumor measured 23 cm at its largest diameter, with histopathological and immunohistochemical features indicating a well-differentiated liposarcoma accompanied by smooth muscle and neural differentiation. Following the resection, the patient underwent radiation treatment and remains alive with no evidence of disease recurrence at two months post-surgery. To the best of our knowledge, the present study is the first to report a case of liposarcoma with smooth muscle and neural differentiation, which indicates that liposarcomas could potentially originate from stem cells. The present study highlights the fact that pathologists must carefully investigate the histopathological characteristics of liposarcomas in order to obtain an accurate diagnosis.

To explore the binding mechanism of phthalate plasticizers with digestive proteases, their effects on conformation and activity of pepsin by multispectroscopic approach and molecular modeling were investigated. Fluorescence spectra combined with UV-vis and circular dichroism (CD) spectra measurements indicated that the six phthalate plasticizers induced the changes of tertiary and secondary structure of pepsin. The solvent polarity of environment around both Trp and Tyr residues on pepsin were affected by phthalate plasticizers. By analyzing the fluorescence quenching and theoretical calculation data, it was concluded that a binding site exists for each phthalate plasticizer in pepsin with different binding ability. The hydrophobic, hydrogen bonding, and π-π stacking interactions were involved in the interactions between pepsin and phthalate plasticizers. Moreover, the activity assay indicated that phthalate plasticizers were not powerfully inhibitors or activators for pepsin. These studies demonstrated that phthalate plasticizers could cause some negative effects on pepsin. The present studies may provide a way to analyze the biological safety of phthalate plasticizers on digestive proteases or other proteins.

Trypsin is one of important digestive enzymes that have intimate correlation with human health and illness. In this work, the interaction of trypsin with methotrexate was investigated by spectroscopic and molecular modeling methods. The results revealed that methotrexate could interact with trypsin with about one binding site. Methotrexate molecule could enter into the primary substrate-binding pocket, resulting in inhibition of trypsin activity. Furthermore, the thermodynamic analysis implied that electrostatic force, hydrogen bonding, van der Waals and hydrophobic interactions were the main interactions for stabilizing the trypsin–methotrexate system, which agreed well with the results from the molecular modeling study.

In recent years, recovering zinc from zinc calcine with high iron has been a matter of discussion. In this paper, sulfuric acid leaching was carried out to assess the effect of several parameters on zinc and iron extraction in zinc calcine with high iron in which the grade of zinc and iron is 53.90% and 19.38%, respectively. Parameters, such as stirring speed, sulfuric acid concentration, liquid to solid ratio and leaching time, were investigated. The results show that leaching time has done nothing to the leaching rate, but has great influence on leaching efficiency. Liquid to solid ratio and sulfuric acid concentration have significant influence on leaching results, and stirring rate has not obvious influence on leaching results. Under the condition of 120g/L sulfuric acid, 6:1 liquid to solid ratio, 55°C leaching temperature and 120min leaching time, the recovery of zinc and iron is 82.24% and 9.64%, respectively. It is obvious that ZnO in zinc calcine is easy to dissolve in acidity solution, which shown in two aspects: high leaching rate and high leaching speed. ZnO can be dissolved entirely in sufficient sulfuric acid in 10min.

In this paper, sulfuric acid leaching was carried out to assess the effect of several parameters on metal extraction in a low grade complex gossan ore in which the grade of zinc and iron is 13% and 40.2%, respectively. Parameters, such as sulfuric acid concentration, liquid to solid ratio and leaching temperature, were studied. The results show that the zinc leaching rate is almost 80%, while the iron leaching rate is about 45% used strong acid with 200g/L. It can be seen from the results that sulfuric acid leaching could not effectively recover zinc from gossan ores studied in this paper because of iron dissolving greatly.

Enterovirus 71 has been implicated in several outbreaks of hand, foot and mouth disease in the Asia-Pacific region. The present study aimed to achieve comprehensive evolutionary dynamic aspects of EV71 during 1994-2013, based on phylogenetic analyses of the VP1 sequences. The results indicated that 4 genotypes, namely C4, C1, C2 and B4 are the predominant strains, especially in Southeast Asian countries. No common ancestor was shared in different countries. Fourteen sites of substitutions were detected in the VP1 gene sequences; including the most common sites related to neutralization at position V249I [47.1% (189/401)] and A289T [42.6% (171/401)]. However, the sites Q22H and Q22R associated with increased virulence were recognized only in 13.7% (55/401) and 18% (72/401), respectively. None of the above mutations seemed to become fixed because the ratio of Ka/Ks was greater than 1.0. Mutations K43E, A58T, S184T, and T240S could possibly change the spatial structure. Two mutations, G145E and T240S, could obviously affect the hydrophobicity of VP1 and thus alter the EV71 immunoreactivity. In conclusion, the VP1 gene of EV71 strains circulating in the Asia-Pacific region during 1994-2013, showed polymorphisms and divergence with very slow evolution rate, which may be one of the reasons for periodic outbreaks in this area.

In recent years, recovering zinc from low grade oxidized zinc ore has been a matter of discussion. In this paper, the investigation for recovering zinc and iron from gossan ores was carried out. In generally, the conventional physical separation methods, such as flotation, gravity separation and magnetic separation, were used to treat the low grade ore, and flotation is the most commonly used method for beneficiation and pretreatment of oxidized zinc minerals. However, gravity separation and magnetic separation are used to deal with the gossan ores because of the special zinc oxide ore bearing iron 40.2% in this study. The effects of grinding fineness and magnetic intensity were investigated, respectively. The results show that the grade of zinc and iron increase slightly, and the best result is that the grade of zinc and iron are 13.35% and 40.25%, the recovery of zinc and iron are 53.45% and 52.19%.

Flotation tests for recovering zinc and iron from gossan ores was carried out in this paper. Flotation is the most commonly used method for beneficiation and pretreatment of oxidized zinc minerals. The effects of grinding fineness, amount of Na2CO3, amount of Na2SiO3, amount of Na2S and amount of octadecylamine were investigated, respectively. The results show that the grade of zinc and iron increase slightly, and the best result is that the grade of zinc and iron are 13.12% and 41.15%, the recovery of zinc and iron are 76.83% and 77.95%. The results simply indicated that flotation could not effectively recover zinc and iron from gossan ores studied in this paper.

Introduction This study examined the validity and reliability of the Patient Health Questionnaire-9 (PHQ-9) and Patient Health Questionnaire-2 (PHQ-2). The optimal cutoff score when screening for depression among Chinese college students was also determined. Methods A total of 959 participants completed the PHQ-9 and the Beck Depression Inventory (BDI) questionnaire. The Structured Clinical Interview for Diagnostic and Statistical Manual of Mental Disorders was used to diagnose depression. Statistical tests were performed to determine the reliability, validity, and receiver operating characteristic curve of the data. The concurrent validity was tested by examining associations between PHQ-9 and BDI. The sensitivity and specificity, as well as the positive and negative predictive values, were calculated for different cutoff scores of PHQ-9 and PHQ-2. Results The internal consistency values of PHQ-9 and PHQ-2 were 0.854 and 0.727, respectively. The test–retest reliability values of PHQ-9 and PHQ-2 were 0.873 and 0.829, respectively. The scores of PHQ-9 (r = 0.790) and PHQ-2 (r = 0.651) were significantly associated with that of BDI. PHQ-9 had an optimal cutoff score of 11, which indicated a sensitivity of 0.89 and a specificity of 0.97, with an area under the curve of 0.977 (95% confidence interval: 0.966–0.988). The PHQ-2 demonstrated satisfactory sensitivity (0.81) and specificity (0.96) at the cutoff score of 3, and its area under the curve was 0.939. Discussion The PHQ-9 and the PHQ-2 are valid and reliable tools to screen depression in Chinese college students. For screening purposes, cutoff scores of 11 and 3 are recommended for PHQ-9 and PHQ-2, respectively.

Methylene blue (MB) was examined for oxidation decoloration. Results indicated that the use of ferrate resulted in effectively removal of chroma which has been explained by oxidation and flocculation interactions. Reaction conditions were optimized concerning ferrate addition mass, reaction temperature and dye concentration. The optimization mass of ferrate and the optimization reaction temperature was observed with decoloration of methylene blue wastewater.

As a promising prevention and therapeutic intervention in Alzheimer’s disease, natural food dyes curcumin could obviously inhibit the generation of Aβ, but the mechanism is not fully defined. This study aims to investigate the effects of curcumin on the amyloidogenic pathway of APP in vitro. Plasmids APPswe and BACE1-mychis were transiently co-transfected into SH-SY5Y and HEK293 cells. Then, they were treated with curcumin at 0, 1.25, 5, 20 μmol/L for 24 h, or with curcumin at 5μmol/L for 0, 12, 24 and 48 h for the time course assay. Our findings showed that curcumin could inhibit the expression of the APP at mRNA level; the expression of the BACE1 and C99 at mRNA and protein levels, furthermore it could inhibit the generation of Aβ40/42, and those changes were dose-time dependent (p

In recent years, the decrease of total arable land area and the area per capita of available arable land resource are serious problems in China. These problems will become more serious with the development of economy. In this paper, based on the statistical data of cultivated land in Hefei city from 1998 to 2009, their driving forces of the cultivated land change were analyzed by means of principal component analysis. The results showed the changing trends. The total and per capita of cultivated land were declining, but the speed of change was not the same. The sharp decrease was occurred in the period of 2002-2005. Nine affecting factors having influenced on cultivated land change which were analyzed by principal component analysis. The results show that population growth, economic development and efficiency of agricultural production were main driving forces affecting cultivated land change in Hefei city.

Photograph method is a new scanning technique that provides cost-efficient and accurate computer system for log centering. A monochrome video camera is used to document images of a log in a perimeters rotation time. 3D digital contour of the log is constructed with the images. The biggest internal cylinder is calculated in software modules. Experiments in present study proved that photograph method was quite feasible in optimizing log centering with higher accuracy. The cost-efficient near-infrared images used in the devices were able to avoid the impact of changing illumination. This centering system can be utilized in small and medium enterprises.

Dietary restriction is a powerful aging intervention that extends the life span of diverse biological species ranging from yeast to invertebrates to mammals, and it has been argued that the antiaging action of dietary restriction occurs through reduced oxidative stress/damage. Using Sod1(-/-) mice, which have previously been shown to have increased levels of oxidative stress associated with a shorter life span and a high incidence of neoplasia, we were able to test directly the ability of dietary restriction to reverse an aging phenotype due to increased oxidative stress/damage. We found that dietary restriction increased the life span of Sod1(-/-) mice 30%, returning it to that of wild-type, control mice fed ad libitum. Oxidative damage in Sod1(-/-) mice was markedly reduced by dietary restriction, as indicated by a reduction in liver and brain F2-isoprostanes, a marker of lipid peroxidation. Analysis of end of life pathology showed that dietary restriction significantly reduced the overall incidence of pathological lesions in the Sod1(-/-) mice fed the dietary-restricted diet compared to Sod1(-/-) mice fed ad libitum, including the incidence of lymphoma (27 vs 5%) and overall liver pathology. In addition to reduced incidence of overall and liver-specific pathology, the burden and severity of both neoplastic and nonneoplastic lesions was also significantly reduced in the Sod1(-/-) mice fed the dietary-restricted diet. These data demonstrate that dietary restriction can significantly attenuate the accelerated aging phenotype observed in Sod1(-/-) mice that arises from increased oxidative stress/damage.

The main inlet component of steam turbine is control valve. The stable operation of the steam turbine control valve is vital for safe and stable operation of the steam turbine and safety production of the power plant. However, due to the complexity of the structure and unsteady characteristics of steam flow in the valve, there is not enough experimental method about the detailed flow characteristics of the area near control valve disc and the inside of the valve chamber up to now. This article is to focus on the simulation of the steam turbine control valve interior flow field which includes the valve pre-inlet channel in different conditions, then find the reasons which caused instability and pressure loss of the control valve by analyzing the flow field details, finally further optimization design. The profile matching of the valve disc and valve seat has a great influence on the interior flow field of control valve, so analysis of the high performance valve disc shape and divergence angle of valve seat is carried out, and the research conclusion is used for guide design and development of the control valve.

The development of computer technology has provided advanced methods for the analysis of complex mechanics problems. Along with the enhancement of computer speed, the computational time used in a finite element analysis is reduced significantly. The main work in a pre-bucking finite element analysis will concentrate on structuring the analytical model and the software model. A normalized factor of the normalized determinant DET of stiffness matrix is defined in this article.The finite element analysis model used in the pre-bucking analysis of laminated composite plates and shells is presented based on the characteristic of the DET. An algorithm for controlling computational procedure and determining critical load is also presented. Numerical examples are given to validate the proposed method and satisfactory results are obtained.

The new insecticide indoxacarb was recently developed with high insecticidal activity in the control of lepidopteran pests, and recent findings demonstrated that indoxacarb also significantly suppressed the fecundity of Nilaparvata lugens Stal (Hemiptera: Delphacidae) populations, a serious insect pest of rice in Asia. The current study investigated the total protein, RNA, and DNA contents in the ovaries and fat bodies of N. lugens adult females after treatment with the insecticide indoxacarb. The results demonstrated that all of the tested concentrations of indoxacarb caused a significant decrease in the protein and RNA contents in the ovaries and fat bodies of the adult females that developed from nymphs fed indoxacarb-treated rice plants compared with those from nymphs fed untreated rice plants, while the DNA contents did not significantly decrease except at 5 days among all of the tested days after emergence. In general, the protein and RNA contents in both the ovaries and the fat bodies decreased linearly with the concentration of indoxacarb applied. There was a significant negative correlation between the total protein and RNA contents and the concentrations of indoxacarb, showing that the total protein and RNA were more sensitive to indoxacarb than was the DNA, and that the changes in RNA contents in turn influence protein synthesis in both the ovaries and fat bodies. The RNA-to-protein ratios in both the ovaries and fat bodies were consistently 0.2 in all of the treatment groups and in the control groups.

The hot rolling, cold rolling and simulative continuous annealing experiments were carried out in the laboratory on the base of new type SFG HSS (super fine grain, high strength steel sheet). The results show that the microstructure which contains a number of cake shaped grain can be refined and homogenized by the feasible annealing holding time. Contrast to the conventional steel, the SFG steel have the characters of super fine grain, high tensile strength, low yield strength/tensile strength rate, good elongation and high r-value high（the plastic strain ratio）．

The present results showed that cooling water (CLW) was more easy to be contaminated than the condensed water (CDW) of central aircondition by Legionella in high air temperature and humidity, and the Legionella was not observed in all sample of the cold medium water (CMW) of central aircondition. The air temperature and relative humidity have significant impact on Legionella. No sample was positive while the average relative humidity was lower than (77.08 ± 1.30) %, and the high value of the ratio of positive samples occurred while the average air temperature and the average relative humidity reached to (26.00 ± 0.35) oC and (85.15 ± 0.74) % respectively. It should be attached importance to the Legionella infection and Legionnaires’ disease prevalence resulted from central aircondition, and the cooling water and condensed water should be sterilized regularly for the prevention and control of Legionella disease.

The adsorption of acetamiprid and phenol in water onto the resin of ZHP02 modified with proline based on NDA150 was investigated from the static experiment at different temperatures in single component system and binary components system. The adsorption capacities and adsorption selectivity for acetamiprid and phenol in waste water were tested. The results showed that the adsorption was fitted by Freundlich model, and the adsorption of acetamiprid or phenol on ZHP02 was an exothermic process. The cooperative adsorption existed in binary components system when the equilibrium concentration was higher. The adsorption of acetamiprid onto ZHP02 had little change in the presence of phenol, but the adsorption of phenol onto ZHP02 was weakened significantly in the presence of acetamiprid. The electrostatic force between acetamiprid and adsorbent was stronger than phenols, so acetamiprid was adsorbed preferentially onto ZHP02.

(1-X)K0.5Na0.5NbO3–xBiGaO3[(1-X)KNN-Xbg] Lead-Free Ceramics with Different Additive of Bigao3 Were Synthesized by Conventional Solid-State Sintering Technique. Dielectric, Piezoelectric and Ferroelectric Properties of (1-X)KNN-Xbg Lead-Free Ceramics Were Studied. it Is Found that the Piezoelectric Properties Was Improved due to the Additive Bigao3，The Ceramics X=0.01 near Room Temperature Exhibit Excellent Electrical Properties D33=152pC/N, TC=372 °C. these Results Indicate that Bigao3 Adjusted K0.5Na0.5NbO3-Based Ceramics Materials Are Promising Lead-Free Piezoelectric Ceramic Candidates for Practical Applications.

Using the 1987 TM and 2006 ETM+ remote sensing images and other related ancillary geographical data of Huainan city, combined with GIS Technology, the dynamic change of the coal mining subsidence of Huainan city was extracted. In order to unify the two period image data difference because of the different date, the research used modified normalized difference water index (MNDWI) to get the information of seeper subsidence in coal mining district. Using the change detection function of RS, Combined with the spatial analysis and statistics function of GIS, all the subsidence area of coal mining district and the seeper subsidence area were extracted. The research shows that the seeper subsidence extent is different because of the mining period, scale, method, groundwater level and groundwater extraction, surface coverage, roof’s management and other coal seam geological environments, especially dip angle of coal seam. The Xiejiaji and Bagongshan mine’s mining seeper subsidence are the serious due to its bigger dip angle of coal seam, the seeper subsidence percentage are 38.57%, 25.95% respectively, while Jiulonggang- Datong mining seeper subsidence is the least even if it’s the oldest and abandoned mine in Huainan.

For Na0.5K0.5NbO3(KNN)-based lead-free piezoelectric ceramics, an important phenomenon was found that the piezoelectric properties can be improved remarkably for equivalence substitution while it can’t be improved remarkably for non-equivalence substitution. To find out the real reason for this phenomenon, Li+ and La3+ were used to modify KNN lead-free piezoelectric ceramics by conventional sintering technique. The relaxor characteristic was detected for La3+ modified KNN lead-free piezoelectric ceramics, and this caused low piezoelectric properties. However, the relaxor characteristic can not be detected for Li+ modified KNN lead-free piezoelectric ceramics, and the piezoelectric properties is higher than that of La3+ modified KNN lead-free piezoelectric ceramics. Accordingly, the relaxor characteristic has important effect on structure and electric properties. The results show that equivalence and non-equivalence substitution have important and different effect on structure and performance of KNN ceramics.

The evaluation index system of water resources sustainable utilization was constructed according to the actual situation of water resources development and utilization in Huaibei City, using the matter element analysis method, analyzed water resources sustainable utilization. The results showed that water resources sustainable utilization status were significantly different in different areas of Huaibei City. The potential of water resources development and utilization was great in Suixi County. The water resources developments of Duji District and Lieshan District have a considerable scale, but there were still some potential for the development and utilization. The degree of water resources utilization was large and water resources carrying capacity was saturated in Xiangshan District. The evaluation result showed that using the matter element analysis method in comprehensive evaluation of water resources development and utilization was feasible.

A data acquisition and monitoring system of machine tools is designed, which is based on Zigbee and GPRS wireless communication technology. The monitoring and alerting about the safety status of the machine tools operates via the acquisition of such physical parameters as temperature and noise. The hardware such as the CC2530 RF circuit and power module is designed. Based on the CC2530 module and SIM900 module, the wireless transmission and SMS alarm can be accomplished. The detailed design process of the software is also given in this paper, besides, the data monitoring is carried out on the PC software platform. It turns out to be a success of this security monitoring system which integrates the data acquisition and transmission of machine tool and alarm system under the industrial environment. This software platform overcomes the shortcomings such as the poor realtime performance of traditional CNC machine tools monitoring system and the difficulty of data transmission, and shows an extensive range of applicability.

To study clinical outcomes of Chinese medidine fumigation and massage therapy for the treatment of knee stability and functional recovery after anterior cruciate ligament reconstruction operation,and to explore the effect on tendon-bone healing.Total 50 patients were divided into two groups: the control group (normal rehabilitation therapy group),the treatment group (Chinese medicine fumigation and manipulation group). There were 25 patients in the control group, including 16 males and 9 females, who were treated with isometric muscle training, with the gradually enlarging amplitude of flexion and progressive loading of bearing training for knee recovery. There were 25 patients in the treatment group, including 15 males and 10 females,who were treated with the conventional rehabilitation therapy combined with Chinese medicine fumigation and massage therapy. The Chinese herbs named as Haitongpi decoction was steamed by a special equipment to fumigate the knee after operation; Based on the biomechanical parameters of the ligament reconstruction, the massage therapy was designed to control the degree of the knee flexion and release the adhesion for early recovery of knee functions. The Lysholm knee function evaluation system was used, and MRI examination was performed to measure the change in width of ligament tunnel in femur and tibia to evaluate the safety and stability of the treatment.Lysholm system showed that two groups both had improving results from the 1st month after operation to the 3rd month (treatment group, F=36.54, P0.05; the control group, F=28.12, P0.05), and the results of the treatment group was better than that of the control group at the observation point (the 1st month, t=0.105, P0.05; the 3rd month, t=5.361, P0.01). There was no difference between the two groups when evaluating the bone and tendon healing 3 and 12 months after operation (P0.05), indicating that Chinese rehabilitation therapy was a safety treatment without the influence on the loosing of tendon.Chinese medicine fumigation and massage therapy can early improve the knee function after the anterior cruciate ligament reconstruction operation without the disturbance of the knee stability.

For the first time, the effects of picoplatin on the structure and esterase-like catalytic activity of human serum albumin (HSA) have been investigated by spectroscopic approaches and molecular modeling. The circular dichroism (CD) spectral examinations indicated that the binding of picoplatin with HSA induced a slight decrease of a-helix content of protein and unfolded the constituent polypeptides of the protein. The synchronous fluorescence and three-dimensional fluorescence spectral methods were used to estimate the effect of picoplatin on the micro-environmental changes of the Trp and Tyr residues of HSA, indicating that the micro-environment around the Tyr and Trp residue is partly disturbed by picoplatin. UV-vis absorption spectral result indicated the formation of the ground state complex between picoplatin with HSA. The ANS binding assay indicated the existence of competitive combination of picoplatin and ANS with HSA. The studies on the effects of picoplatin on the binding of HSA with bilirubin and heme showed that picoplatin binding caused a change of angle between two chromophores of bound bilirubin and the binding site of picoplatin does not locate in subdomain IB in HSA that bound with heme. The molecular modeling results showed that picoplatin binds to the connection between domain I and domain II by hydrophobic, hydrogen bonds, and van der Waals forces. In addition, HSA maintains most of its esterase activity in the presence of picoplatin. The investigations on how picoplatin interacts with HSA are important for the understanding of the anticancer mechanism and toxicity of platinum-based anticancer drug.

According to the existing domestic and foreign research results as well as the characteristics of the guide fund, this paper constructs the index system of guide fund performance evaluation, selects the data of the part of the enterprises shared by the government guide venture capital fund and uses the factor analysis method to optimize the evaluation model, then uses empirical analysis based on DEA-BCC model. The result shows that the optimized evaluation model can fully evaluate the performance of the guide fund. At the same time, empirical analysis based on DEA-BCC model shows that the results can objectively reflect the operating conditions of companies accepted investment, which provides the objective reference for government venture capital fund and companies accepted investment.

The epidermal growth factor receptor (EGFR) belongs to ErbB family of tyrosine kinases, which plays an important role in the regulation of cell proliferation, survival, differentiation, and metastasis. In addition, tumors develop resistance to EGFR inhibitors due to the presence or development of point mutations in the tyrosine kinase domain of the receptor. There is an urgent need to develop new anti-EGFR agents with strong therapeutic activities. As the majority of anticancer drugs are of natural origin, natural products are a valuable source for the identification and development of novel treatment options for cancer. To identify promising new anti-tumor agents that act on the EGFR, numerous studies have been carried out and some investigators found natural products that could effectively inhibit the EGFR. This review summarizes the latest developments related to anti- EGFR agents derived from natural compounds and traditional Chinese medicine (TCM).

Perfluorooctane sulfonate (PFOS) is among the most prominent contaminates in human serum and has been reported to possess potential toxicity to the human body. In this study, the effects of PFOS on the conformation and activity of bovine serum albumin (BSA) were investigated in vitro. The results indicated that the binding interaction of PFOS with BSA destroyed the tertiary and secondary structures of protein with the loss of α-helix structure and the increasing of hydrophobic microenvironment of the Trp or Tyr residues. During the thermal denaturation protein, PFOS increases the protein stability of BSA. The proportion of α-helix decreased on increasing the PFOS concentration and the microenvironment of the Trp or Tyr residues becomes more hydrophobic. The results from molecular modeling indicated that BSA had not only one possible binding site to bind with PFOS by the polar interaction, hydrogen bonds and hydrophobic forces. In addition, the BSA relative activities were decreased with the increase of PFOS concentration. Such loss of BSA activity in the presence of PFOS indicated that one of the binding sites in BSA is located in subdomain IIIA, which is in good agreement with the fluorescence spectroscopic experiments and molecular modeling results. This study offers a comprehensive picture of the interactions of PFOS with serum albumin and provides insights into the toxicological effect of perfluoroalkylated substances.

The mechanical and electronic products especially the battery bring the great convenience to our lives, but also consume a large amount of resource, and pollute environment. In this paper we analysis the problems in battery recycling and utilization in China, and point out the shortcomings as well as propose the construction of battery full life cycle green recycling system.

The project team is a very important form of modern organization and is becoming a source of organizational creativity. Team reflexivity, also known as team introspection, has become a Research hostpot. Study about the relationship between project team reflexivity and team performance and open the project team management "black box" has important theoretical and practical significance. As the project team be in a dynamic market environment which the product and technology updates fast, so environmental dynamism will have an impact on the relationship between team reflexivity and team performance. Therefore, the paper brings team reflexivity into the field of project team on the base of team reflexivity theory. Testing the hypotheses on 100 project teams, research shows that the project team reflexivity has a positive effect on team performance, with this effect mediated by workload sharing and team helping, especially under conditions of stronger environmental dynamism. Additionally, we put forward the managerial implications and suggest possible future research directions.

It’s a real problem to visit a low-level device driver from a high-level application based on the .NET platform managed code in software developing practice .This paper concerns how to solve it by a runtime interoperation services provided by Microsoft .NET Common Language Runtime (CLR) .A real world example is given in the end.

The new type SFG HSS (super fine grain, high strength steel sheet) has been developed by adding solid-solution strengthening elements to conventional IF steel such as Si, Mn. The precipitation behavior of the second phase particles was observed and studied by a carbon extraction replica technique used transmission electron microscopy (TEM). The shape of the small second particles is similar to spherical and ellipse, the sizes of which are 10~30nm. It is seen that the particles are dispersed on the matrix. The precipitate composition of small particles is Nb (CN) and the precipitate composition of large particles is NbC examined by energy dispersive X-ray (EDX). It is noted that the yield strength is low as well as the tensile strength is high by the PFZ which is free of precipitate called precipitated free zone on the one side of the grain boundary. The results of microstructure shows that the second phase particles pinned on grain boundary not only can inhibit the grain growth, but also the grain can be fined.

Water resources carrying capacity (WRCC) is an important factor which influences development speed and scale of society and economy. The evaluation index system of WRCC was constructed according to municipal economic development status and the utilization of water resources in Suzhou City, using the fuzzy comprehensive evaluation method, analyzed water resources carrying capacity in different years. The results showed that water resources carrying capacity has tended to be smaller in recent years. It indicated that the development and utilization of water resources were large, water resources carrying capacity was close to saturation and the development potential of water resources was small in Suzhou City.