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Objetivo: Este estudo verificou a aderência de idosos da comunidade a um programa de exercício físico aquático e quais fatores sociodemográficos e clínicos influenciam na aderência ao programa. Métodos: Trata-se de dados de um ensaio clínico controlado randomizado, unicêntrico, constituído por dois grupos (Grupo Exercício Físico Aquático, GEFA, e Grupo Controle, GC).Os participantes participaram de uma avaliação inicial, contendo anamnese, nível de atividade física (Questionário Baecke Modificado para Idosos), Exame Cognitivo de Addenbrooke – versão revisada, Escala de Depressão Geriátrica Abreviada, Falls Efficacy Scale-International e teste Timed up and go. O GEFA participou de um programa de exercício físico aquático 2 vezes/semana com uma hora de duração cada sessão por 16 semanas. No fim da 16 semanas, os participantes do GEFA responderam a 11 questões objetivas e 1 descritiva referentes a aderência ao programa.Considerou-se como aderência satisfatória frequência igual ou superior a 50% aos exercícios. 24 idosas com idade acima de 65 anos e apresentando até 2 comorbidades participaram deste estudo . O presente estudo foi aprovado pelo Comitê de Ética em Pesquisa (CEP/ UFMS), sob CAAE 03898218.0.1001.0021. Resultados: O GEFA apresentou uma média de 52,2% de frequência ao programa de exercícios, com 10 idosas apresentando aderência satisfatória e 4 idosas ficaram próximas.Os idosos relataram melhora na saúde, principalmente em relação a funcionalidade e das dores,da atenção para a realização de múltiplas atividades, também quanto a relacionamento com colegas e ótima relação com a fisioterapeuta, descrevendo ser simpática, dedicada,atenciosa, ademais,com explicações de fácil entendimento para execução eficaz.Conclusão: Quanto aos resultados apresentados houve correlação significativa negativa entre o risco de depressão e a aderência dos participantes.Sugere-se que embora a baixa adesão, às idosas preferem os exercícios aquáticos. Ademais, o menor risco de depressão pode influenciar positivamente na adesão do idoso ao exercício aquático. Palavras-chave: Envelhecimento. Aderência. Exercício físico aquático. Fisioterapia

Background Wearable monitoring devices based on inertial sensors have the potential to be used as a quantitative method in clinical practice for continuous assessment of gait disabilities in Parkinson’s disease (PD). Methods This manuscript introduces a new gait monitoring system adapted to patients with PD, based on a wearable monitoring device. To eliminate inter- and intra-subject variability, the computational method was based on heuristic rules with adaptive thresholds and ranges and a motion compensation strategy. The experimental trials involved repeated measurements of walking trials from two cross-sectional studies: the first study was performed in order to validate the effectiveness of the system against a robust 3D motion analysis with 10 healthy subjects; and the second-one aimed to validate our approach against a well-studied wearable IMU-based system on a hospital environment with 20 patients with PD. Results The proposed system proved to be efficient (Experiment I: sensitivity = 95,09% and accuracy = 93,64%; Experiment II: sensitivity = 99,53% and accuracy = 97,42%), time-effective (Experiment I: earlies = 13,71 ms and delays = 12,91 ms; Experiment II: earlies = 12,94 ms and delays = 12,71 ms), user and user-motion adaptable and a low computational-load strategy for real-time gait events detection. Further, it was measured the percentage of absolute error classified with a good acceptability (Experiment I: 3,02 ≤ e%≤12,94; Experiment II: 2,81 ≤ e%≤13,45). Lastly, regarding the measured gait parameters, it was observed a reflection of the typical levels of motor impairment for the different disease stages. Conclusion The achieved outcomes enabled to verify that the proposed system can be suitable for gait analysis in the assistance and rehabilitation fields.

Background Transcatheter aortic valve replacement (TAVR) is often associated with intraventricular conduction disturbances. We aimed to determine the association between implantation depth assessed by multidetector computed tomography (MDCT) and new-onset conduction abnormalities after TAVR. Methods Retrospective single-center study including patients consecutively submitted to TAVR, between August/2007 and October/2016, who underwent routine MDCT 3 months after the procedure. The endpoint of conduction disturbances included permanent pacemaker implantation and/or new-onset left bundle-branch block. Implantation depth was determined as the distance between the ventricular end of the prothesis and the native ring, at the level of the non-coronary cusp. Results 138 patients were included (female gender 52.2%, mean age 78.7 ± 6.9 years). The EuroSCORE II was 4.0 ± 3.9% and 57.2% were treated with self-expanding prosthesis. The endpoint of conduction abnormalities was found in 45.7% (n = 63). The implantation depth was greater in the group with conduction disturbances (7.7 vs 6.4 mm, p = 0.006). Chronic obstructive pulmonary disease, oversizing and implantation depth were independent predictors of conduction abnormalities. Implantation depth had an AUC of 0.64 (p = 0.004) for the prediction of conduction abnormalities and a cut-off value of 7.1 mm predicted the composed endpoint with a sensitivity and specificity of 65% and 70%, respectively. Conclusions Implantation depth assessed by MDCT is associated with new-onset conduction disturbances after TAVR. In patients with conduction abnormalities, which do not qualify for the immediate implantation of pacemaker, the assessment of implantation depth by MDCT may be an additional marker of risk to aid decision-making.

Introduction: Atrial fibrillation (AF) is the most common sustained arrhythmia in clinical practice and a major cause of morbidity, due to the associated risk of stroke. However, since it is often paroxysmal, it is commonly underdiagnosed and undertreated. Objectives: The primary objective of this prospective study was to determine the prevalence of paroxysmal atrial fibrillation (PAF) in patients aged 40 and above in a population who underwent continuous 24-hour electrocardiographic monitoring. The secondary objectives were to determine the overall prevalence of AF/atrial flutter (AFL) regardless of the type and to compare the population with AF with the general population and patients with PAF with patients with AF. Results: A total of 4843 consecutive patients were analyzed, 58% women, 26.2% aged 70-79 years (n=1269), 25.9% (n=1252) aged 60-69 years, and 19.0% (n=923) aged 50-59 years; the others were aged either >80 years (n=712, 14.7%) or 80 age-groups) (p80 age-groups) (p 80 anos) (p 80 anos (p

The site of the sinus tract depends on the rate of resistance against abscess exudate drainage, bone morphology, and distance from the root apex to the outer cortical bone. Objective To assess apical bone thickness in buccal and palatal/lingual aspects of maxillary and mandibular teeth, using a high-resolution cone-beam computed tomography (CBCT) system. Methodology In total, 422 CBCT examinations were included in the study, resulting in a sample of 1400 teeth. The scans were acquired by PreXion 3D, with a high-resolution protocol. The bone thickness was taken as the distance between the center of the apical foramen and the buccal and lingual/palatal cortical bone. The quantitative variables were expressed as mean values±standard deviation. The independent samples were analyzed using the t-test or the Mann-Whitney test (p

Zika is an important emerging infectious disease in which the role of T cells remains elusive. This study aimed to evaluate the phenotype of multifunctional T cells in individuals 2 yr after exposure to Zika virus (ZIKV). We used a library of 671 synthetic peptides covering the whole polyprotein of ZIKV in pools corresponding to each viral protein (i.e., capsid, membrane precursor or prM, envelope, NS1 [nonstructural protein], NS2A + NS2B, NS3, NS4A + NS4B, and NS5) to stimulate PBMCs from individuals previously exposed to ZIKV. We observed an increased frequency of ZIKV-specific IFNγ, IL-17A, TNF, and IL-10 production by T cell populations. IFNγ and TNF production were especially stimulated by prM, capsid, or NS1 in CD8+ T cells and by capsid or prM in CD4+ T cells. In addition, there was an increase in the frequency of IL-10+ CD8+ T cells after stimulation with prM, capsid, NS1, NS3, or NS5. Multifunctional properties were observed in ZIKV-specific T cells responding especially to prM, capsid, NS1 or, to a smaller extent, NS3 antigens. For example, we found a consistent IFNγ + TNF+ CD8+ T cell population in response to most virus antigens and CD4+ and CD8+ T cells that were IFNγ + IL-17A+ and IL-17A+IL-10+, which could also produce TNF, in response to capsid, prM, NS1, or NS3 stimulation. Interestingly, CD8+ T cells were more prone to a multifunctional phenotype than CD4+ T cells, and multifunctional T cells were more efficient at producing cytokines than single-function cells. This work provides relevant insights into the quality of ZIKV-specific T cell responses and ZIKV immunity.

PurposeThe purpose of this paper is to contribute to the relaxion on what can be done to develop ethical cultures that may be less permeable and more resilient to changes in leadership from an ethical point of view. The influence of leaders on organisational ethics is recognised, and there are even those who consider that it is not possible to maintain an ethical culture when leaders are not engaged. But, if this theory is true, all business ethics programmes that can be created, and the cultures that can gradually be developed in organisations, will always have their existence and robustness suspended at each leadership change. How to maintain an ethical culture beyond leadership?Design/methodology/approachAs a strategy, we used the case study with a narrative methodology, in which a chief executive officer (CEO) and a chief compliance officer (CCO) narrate in the first person a case of perceived collapse of the ethical culture of a multinational company.FindingsThe findings point to the difficulty in maintaining ethical leadership. Key aspects to protect an organization from leadership changes are as follows: the management of the succession process, the quality of the training on ethics and the mechanisms developed by the organization to foment speak up and take notice of the situations. Moral blindness and the banality of evil that also can be observed in organizations appear as facilitating elements for collapse.Originality/valueEthical leadership is generally presented as a necessary condition for an ethical culture. However, leaders often have unethical or ethically neutral leadership. This case helps to understand the difficulties experienced by leaders in adopting ethical leadership and proposes a set of instruments and procedures that, when included in an ethical programme, can protect the company's ethical culture against unethical leaders. Some characteristics of our case study make it particularly relevant: action occurs in a multinational, a context where, by size and complexity, achieving uniformity in culture becomes particularly relevant, and actions happen in the context of a CEO succession process, something that may occur in any company and which is often a trigger for ethical misconducts. Additionally, our case is narrated by a CEO and a CCO, which makes it rare, as it is especially difficult to have access to these executives.

Novel electrolytes composed of chondroitin sulfate A (CSA) and citric acid (CA) have been prepared using a clean, safe, and fast route. These electrolytes exhibit different physical-chemical properties, depending on the amount of CA. For X > 82.3%, where X is the mass ratio, in %, of CA/(CA + CSA), whitish polycrystalline powders result. Lower amounts of CA leads to the production of translucent, amorphous films, sticky for X = 75.6 and 82.0, brittle for X < 43.6 and crack-free, self-standing for 43.6 < X < 75.6%. The results obtained provide evidence that, at low pH, strong hydrogen bonding interactions take place between the anionic sulfonic and carboxylic groups of CSA and CA. CA exerts a key role, acting as a cross-linker and proton source, while simultaneously influencing sample morphology. At room temperature the highest ionic conductivity is achieved at X = 60.8%. A significant enhancement of the ionic conductivity of this sample occurs with the increase of relative humidity (RH) (from 3.1 × 10−7 to 3.7 × 10−2 S cm−1 30% for RH = 30 and ~100%, respectively)., Support by Fundação para a Ciência e Tecnologia, I.P. (FCT) in the framework of the Strategic Funding UID/FIS/04650/2013, UID/QUI/ 00686/2013, UID/QUI/50006/2019 and UID/QUI/00686/2016. This work was funded by the R&D Project UniRCell-Unitised regenerative fuel cell for efficient renewable energy supply: from materials to device, with reference POCI-01-0145-FEDER-016422 and SAICTPAC/0032/ 2015, financed by the European Regional Development Fund (ERDF) through COMPETE 2020 – Operational Program for Competitiveness and Internationalization (POCI) and by the Foundation for Science and Technology (FCT) and CICECO-Aveiro Institute of Materials (UID/ CTM/50011/2019), financed by national funds through the FCT/MEC. Is also acknowledged, as well as funding under projects UID/CTM/ 50025/2013, Pest-OE/QUI/UI0616/2014, and LUMECD (PTDC/CTM/ NAN/0956/20149 and POCI-01-0145-FEDER-016884). F. M. Santos acknowledges a Post-PhD Fellow grant supported by Project UniRcell. P. C. Barbosa employment contract is funded by national funds (OE), through FCT – Fundação para a Ciência e a Tecnologia, I.P., in the scope of the framework contract foreseen in the numbers 4, 5 and 6 of the article 23, of the Decree-Law 57/2016, of August 29, changed by Law 57/2017, of July 19.R. F. P. Pereira Post-PhD fellow was funded by FCT (SFRH/BPD/87759/2012). S. C. Nunes was funded by FCT projects (Post-PhD Fellowships of UniRCell and LUMECD projects). H.M.R. Gonçalves was funded by NORTE-01-0145-FEDER-030858 and PTDC/ BTM-MAT/30858/2017.

Due to the technological evolution on wearable devices, biosignals, such as inter-cardiac beat interval (RR) time series, are being captured in a non-controlled environment. These RR signals, derived from photoplethysmography (PPG), enable health status assessment in a more continuous, non-invasive, non-obstructive way, and fully integrated into the individual’s daily activity. However, PPG is vulnerable to motion artefacts, which can affect the accuracy of the estimated neurophysiological markers. This paper introduces a method for motion artefact characterization in terms of location and relative variation parameters obtained in different common daily activities. The approach takes into consideration interindividual variability. Data was analyzed throughout related-samples Friedman’s test, followed by pairwise comparison with Wilcoxon signed-rank tests with a Bonferroni correction. Results showed that movement, involving only arms, presents more variability in terms of the two analyzed parameters.

Made available in DSpace on 2020-12-12T02:43:37Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-01-01 Introduction/objective: To evaluate the effects of a periodized circuit training (CT) compared with a conventional strength training (ST) and an educational protocol (EP) on body composition, metabolic parameters, muscle strength, pain, and physical performance in patients with knee osteoarthritis (KOA). Method: This study followed a randomized controlled trial design. A sample of sixty-one patients with KOA, 40–65 years old and BMI < 30 kg/m2, were randomly divided into three 14-week protocols: CT, ST, or EP. The CT performed whole body exercises organized in circuit, 3 times a week. The ST performed conventional resistance exercises also 3 times a week, and the EP joined in educational meetings twice a month. Body composition (dual-energy X-ray absorptiometry (DXA)); metabolic parameters (abdominal obesity, triglyceride, HDL, blood pressure and fasting glucose), knee maximal isometric voluntary contraction (MIVC) extension and flexion, performance-based tests (40-m walk test, 30-s chair test, and stair climb test), self-reported pain, stiffness, and physical function (WOMAC) and pain catastrophism were evaluated at baseline and follow-up. Repeated measures ANOVA were used to compare differences between groups (CTxSTxEP) at the different times of assessment (baseline × follow-up). When group–time interaction was found, a one-way ANOVA, followed by a Bonferroni post hoc test, was used to compare groups in each time point; while a paired t test was used to verify the time effect in each group. Additionally, the training effect was analyzed in terms of the percent change (Δ%). Effect size was calculated for post intervention (week 14) between groups. For all analyses a significance level of 5% was adopted (p < 0.05). Results: The CT was the only protocol that presented significant lower body mass (p = 0.018; Δ% = − 1.4), fat mass (p = 0.017; Δ% = − 10.3), %fat mass (p < 0.001; Δ% = − 13.9), adipose indices (%fat trunk/%fat legs p = 0.031; Δ% = 7.8; fat mass/height2p = 0.011; Δ% = − 15.1; trunk/limb fat mass p = 0.012; Δ% = − 8.2; visceral adipose tissue (VAT) volume (cm3) p = 0.039; Δ% = − 11.6; VAT area (cm2) p = 0.044; Δ% = − 12.2), blood pressure levels (SBP p = 0.042; Δ% = − 3.4 and DBP p = 0.001; Δ% = − 4.9), higher lean mass and HDL (p < 0.001; Δ% = 5.9) compared to baseline. CT and ST presented lower WOMAC pain, stiffness and physical function (p < 0.001; Δ% = − 64.0 for CT and p < 0.001; Δ% = 0.001; Δ% = − 46.4 for ST in WOMAC total score, pain catastrophizing (p < 0.001; Δ% = − 66.7 for CT and p < 0.001; Δ% = − 61.5 for ST) a higher functional performance (30 s chair test: p < 0.001; Δ% = 45.7 for CT and p < 0.001; Δ% = 26.9 for ST; 40 m walk test: p < 0.001; Δ% = 26.7 for CT and p < 0.001; Δ% = 12.5 for ST; stair climb test: p = 0.002; Δ% = − 33.9 for CT and p < 0.001; Δ% = − 18.8 for ST) and knee extension MIVC (p < 0.001; Δ% = 21.92 for CT and p < 0.001; Δ% = 30.25 for ST). Conclusion: A periodized CT model reduces visceral adipose tissue, SBP and DBP, and improves HDL, important cardiovascular and metabolic risk factors, in patients with KOA. Both trained groups (CT and ST) improve self-reported clinical symptoms, muscle strength, and physical performance in this sample. Trial Registration number: ClinicalTrials.gov, NCT02761590 (registered on May 4, 2016).Key points:• A 14-week periodized circuit training reduces visceral adipose tissue, systolic and diastolic blood pressure, and improves HDL blood levels in patients with knee osteoarthritis.• Both periodized circuit training and conventional strength training improve clinical symptoms, functional performance and muscle strength in this sample. Department of Physiotherapy Federal University of São Carlos (UFSCar) Integrated Health Institute Federal University of Mato Grosso do Sul (UFMS) Department of Physiotherapy São Paulo State University (UNESP) Department of Physiotherapy São Paulo State University (UNESP)

O artigo tem como objetivo explicitar os estratagemas psicológicos utilizados pelo Movimento Escola sem Partido em seus projetos de lei, desvelando as estratégias psicológicas empregadas para suscitar concordância e adesão dos sujeitos aos discursos e métodos do Movimento. Realizou-se uma análise de conteúdo dos projetos de lei do Movimento apresentados na esfera federal. Foi possível constatar que o Movimento utiliza reiteradamente de estratagemas típicos da propaganda totalitária, que buscam se aproveitar da debilidade dos sujeitos na sociedade que os predispõe a discursos autoritários. Ainda que os projetos não tenham sido aprovados na esfera federal, seus discursos produziram efeitos no campo das políticas públicas de educação, bem como nas escolas e na sociedade. Embora haja certa imprecisão quanto aos seus objetivos, é possível concluir que o Movimento busca impedir a experiência mais básica de uma educação diversa e democrática, impondo a uniformização do pensamento.

Agradecimentos: Gostaríamos de agradecer ao Prof. Dr. José Joaquín Lunazzi que contribuiu com frutíferas discussões na etapa inicial desse trabalho; ao engenheiro Antonio Carlos da Costa pela disponibilidade de um aparato experimental para primeiros testes; ao Vladimir Gaal pela disponibilidade no auxilio com a montagem dos lasers; ao Rafael Hensel pelo auxilio com as fotos; ao Instituto de Física "Gleb Wataghin"da Unicamp pela infraestrutura disponibilizada para o desenvolvimento deste trabalho Abstract: Neste trabalho apresentamos um aparato de baixo custo, lúdico e de fácil acesso para experimentação na área de óptica geométrica. A simplicidade da montagem e a facilidade do manuseio favorecem a aplicação deste projeto, que se propõe a construir lentes esféricas com gelatina comestível. Com o auxilio de gabaritos de papel, feitos no formato desejado, foi possível cortar a gelatina de modo que ela ficasse no formato de uma lente esférica. Utilizando esses materiais, medimos o índice de refração da gelatina a partir da Lei de Snell e medimos a distância focal de uma lente convergente. Também foi possível realizar uma simulação do funcionamento do cristalino presente no olho humano. Ao esticar e comprimir a lente de gelatina é possível observar a alteração da posição do seu foco, ilustrando o mecanismo de acomodação que ocorre em nossos olhos Aberto

The success of lithium ion batteries (LIBs) and their unique advantages for electrochemical energy storage have speed up research in this field. A critical component of LIBs is the separator. Here, Bombyx mori silk cocoon separators have been treated with oxygen (O2) and nitrogen (N2) plasmas at different exposure times. The goal was to improve the electrochemical characteristics of these natural separators without jeopardizing the major attributes of silk fibers. Major physical and chemical modifications have been identified at the submicrometer scale upon the application of the plasmas: (1) Etching of the silk nanofibrils and concomitant increase of roughness (more effective with O2 plasma), corresponding to the destruction of the 5 th structural hierarchy level of the silk fibers. (2) Creation of oxygen-containing functional groups carrying negatives charges at the surface, causing a superhydrophic-to-superhydrophilic transition, and favoring Li+ transport. The optimized cocoon separator exposed to O2 plasma for 30 s exhibited high electrolyte uptake (289%), high ionic conductivity (2.33 mS cm−1 at 25 ºC) and low overall resistance. A cathodic half-cell of carbon-coated lithium iron phosphate incorporating this separator sample soaked in the ethylene carbonate/dimethyl carbonate/lithium hexafluorophosphate electrolyte demonstrated a performance boost with respect to the battery including a raw cocoon separator: an outstanding increase (ca. 270%) of the discharge capacity (from 26.1 to 96.7 mAh g−1 at 5C-rate) and an impressive increase (291%) of the capacity retention (from 22.3 to 87.2%, from C/5 to 5C). This work proves that the O2 plasma exposure is a valid, simple, fast, clean and safe top-down methodology to improve the properties of Bombyx mori cocoon separators. The features of these plasma-treated separators, which surpass those of commercial separators, help upgrading the performance of LIBs. This plasma-enhanced natural biomaterial separator technological platform pushes LIBs to the next performance-level, while guaranteeing the eco-friendly, sustainability and safety labels., This work was supported by National funds by Foundation for Science and Technology (FCT) in the framework of the Strategic Funding UID/FIS/04650/2019, UID/QUI/00686/2016, UID/QUI/00686/2018 and UID/QUI/00686/2019. The authors thank FEDER funds through the COMPETE 2020 Program and National Funds through FCT under the projects PTDC/FIS-MAC/28157/2017, PEst-OE/QUI/UI0616/2014, LUMECD (POCI-01-0145-FEDER-016884 and PTDC/CTM-NAN/0956/2014), UniRCell (SAICTPAC/0032/2015 and POCI-01-0145- FEDER-016422), Investigator FCT Contract CEECIND/00833/2017 (R.G.) and grants SFRH/BPD/87759/2012 (R.F.P.P), SFRH/BPD/121526/2016 (D.M.C) and SFRH/BPD/112547/2015 (C.M.C). R.F.P.P. also thanks FCT-UM for the researcher contract in the scope of Decreto-Lei 57/2016 and 57/2017. H.M.R. acknowledges financial support from PTDC/BTM-MAT/30858/2017. Funding by Basque Government Industry and Education Department under the ELKARTEK, HAZITEK and PIBA programs is acknowledged.

ObjectiveTo estimate knee osteoarthritis (OA) risk following anterior cruciate ligament (ACL), meniscus or combined ACL and meniscus injury.DesignSystematic review and meta-analysis.Data sourcesMEDLINE, Embase, SPORTDiscus, CINAHL and Web of Science until November 2018.Eligibility criteria for selecting studiesProspective or retrospective studies with at least 2-year follow-up including adults with ACL injury, meniscal injury or combined injuries. Knee OA was defined by radiographs or clinical diagnosis and compared with the contralateral knee or non-injured controls.Study appraisal and synthesisRisk of bias was assessed using the SIGN50 checklist. ORs for developing knee OA were estimated using random effects meta-analysis.Results53 studies totalling ∼1 million participants were included: 185 219 participants with ACL injury, mean age 28 years, 35% females, 98% surgically reconstructed; 83 267 participants with meniscal injury, mean age 38 years, 36% females, 22% confirmed meniscectomy and 73% unknown; 725 362 participants with combined injury, mean age 31 years, 26% females, 80% treated surgically. The OR of developing knee OA were 4.2 (95% CI 2.2 to 8.0; I2=92%), 6.3 (95% CI 3.8 to 10.5; I2=95%) and 6.4 (95% CI 4.9 to 8.3; I2=62%) for patients with ACL injury, meniscal injury and combined injuries, respectively.ConclusionThe odds of developing knee OA following ACL injury are approximately four times higher compared with a non-injured knee. A meniscal injury and a combined injury affecting both the ACL and meniscus are associated with six times higher odds compared with a non-injured knee. Large inconsistency (eg, study design, follow-up period and comparator) and few high-quality studies suggest that future studies may change these estimates.Clinical relevancePatients sustaining a major knee injury have a substantially increased risk of developing knee OA, highlighting the importance of knee injury prevention programmes and secondary prevention strategies to prevent or delay knee OA development.PROSPERO registration number CRD42015016900

Objective: This study aimed to investigate the relationship of hip abductor strength with external hip and knee adduction moments, pain and physical function, and trunk, pelvis, and hip kinematics in the frontal plane during walking in subjects with medial knee osteoarthritis. \ud Methods: Twenty-five subjects with medial knee osteoarthritis were evaluated through an isokinetic strength test for hip abductor, three-dimensional gait analysis (kinetics and kinematics), pain and physical function scores. Regression models were used to control the influence of other parameters such as pain, age, gender, severity, walking speed, mass and height. \ud Results: No relationship was found of hip abductor strength with peak of external knee adduction moment and knee adduction angular impulse. Hip abductor strength explained 17% of contralateral pelvic drop and 21% of hip adduction angle. In addition, hip abductor strength explained 4% and 1% of the variance in the WOMAC physical function score and 40 meter fast paced walk test, respectively. \ud Conclusion: Considering the relationship of hip abductor strength with contralateral pelvic drop and hip adduction angle, specific exercises might improve physical function and lower limb dynamic alignment during gait.

Atrial fibrillation (AF) is the most prevalent chronic arrhythmia and a major cause of stroke and mortality. It is thought to confer an overall 5-fold increased risk of a cerebrovascular event, causing ≈one-third of all ischemic strokes. Half of the 2 to 3 fold higher risk of mortality among AF patients is related to AF itself, not only via fatal progression of heart failure, the most frequent mode, but also sudden death and embolic events.1,2 Importantly, AF patients who suffer a cardioembolic stroke have a worse outcome compared with stroke patients without AF. Anticoagulation has been shown to reduce the risk of a cerebrovascular event in AF patients. However, despite adequate anticoagulation, some patients remain at risk of stroke. Whether successful catheter ablation can reduce this risk remains unclear. Although there has not been any convincing evidence thus far that AF ablation leads to a reduction in the risk of stroke, no randomized study was powered to address this question. In this review article, we discuss the AF-stroke association, as well as the apparent lack of evidence supporting the use of ablation for the specific reduction of this end point.

This article presents the results of a research developed in the Conservatory of Vale do Sousa on the teaching of music in an articulated regime of attendance. This body of work focused on questioning the concept of "vocational" school, testing possible redefinitions of teaching music in an articulated regime of attendance and studying the advantages of reinforcing a pedagogical articulation of the specialized subsystem and the general schools. The Action Research part of the project held two work groups: one was in a specialized school (called vocational school in Portugal) and the other in a general school. The research allowed us to conclude that, in the context in study, it is not possible to tell apart, based on musical results, the population that attended a specialized school and a general school, questioning thus the concept of "vocational" schools; the type of teaching in "vocational" schools and in general schools is deep in ambiguity; the instrumental pedagogy in a group proved to be an effective method. The specialized teaching of music needs, therefore, to contemplate pedagogical and didactical strategies that allow for diversity in learning approaches, to introduce multiple music genres and to make possible for all children to learn a music instrument. Este artículo presenta los resultados de una pesquisa llevada a cabo en el Conservatorio do Vale do Sousa en torno de la enseñanza de la música en régimen articulado. Este proyecto ha pretendido cuestionar el concepto de escuela vocacional, testar posibles redefiniciones de la enseñanza de la música en régimen articulado y estudiar las ventajas del refuerzo de las articulaciones pedagógicas de la enseñanza vocacional con la enseñanza general. La Investigación-Acción ha corporeizado el proyecto y ha contemplado dos grupos de trabajo: uno de enseñanza vocacional y otro de enseñanza general. La pesquisa ha permitido concluir que, en el contexto en estudio, no es posible diferenciar, a través de los resultados musicales, la población que frecuenta la escuela especializada y la escuela general, poniendo en causa el concepto de escuela vocacional; la enseñanza de la música en la escuela vocacional y en la escuela general se encuentra sumergida en una ambigüedad profunda; la pedagogía instrumental en grupo se ha revelado una metodología eficaz. La enseñanza especializada de la música necesita, así, de contemplar estrategias pedagógicodidácticas de diversificación de los recorridos de aprendizaje, introducir múltiples tipologías musicales y posibilitar a todos los niños el aprendizaje de un instrumento musical. Este artigo apresenta os resultados de uma pesquisa desenvolvida no Conservatório do Vale do Sousa em torno do ensino da música em regime articulado. Este trabalho pretendeu questionar o conceito de escola vocacional, testar possíveis redefinições do ensino da música em regime articulado e estudar as vantagens do reforço das articulações pedagógicas do ensino vocacional com o ensino genérico. A investigação-ação corporizou o projeto e contemplou dois grupos de trabalho: um do ensino vocacional e outro do ensino genérico. A pesquisa permitiu concluir que, no contexto em estudo, não é possível diferenciar, através dos resultados musicais, a população que frequenta a escola especializada e a escola genérica, colocando em causa o conceito de escola vocacional; o ensino da música na escola vocacional e na escola genérica encontra-se mergulhado em uma ambiguidade profunda; a pedagogia instrumental em grupo revelou-se uma metodologia eficaz. O ensino especializado da música necessita, assim, contemplar estratégias didático-pedagógicas de diversificação dos percursos de aprendizagem, introduzir tipologias musicais diversificadas e possibilitar a todas as crianças a aprendizagem de um instrumento musical.

Um porto é uma organização dinâmica que possui áreas críticas que abrigam fontes emissoras de poluentes, como indústrias em seu entorno, modais terrestres (caminhões e trens), operações com carga e descarga de granéis agrícolas, fluxo de navios, entre outros. Neste estudo foi utilizada a modelagem desenvolvida em 2009 pela Current Methodologies in Preparing Mobile Source Port-Related Emission Inventories da Agência Americana de Proteção ao Meio Ambiente (US EPA), que estima os fatores de emissão de material particulado por navios considerando a energia, a característica e o tempo de atividade de cada embarcação, o fator de carga de propulsão e o fator de emissão para cada poluente. Para estimar as emissões fugitivas de material particulado provenientes de operações portuárias com milho, grãos de soja e farelo de soja foi utilizada a modelagem desenvolvida em 2006 pelo Group Emissions Factor US EPA, que considera o volume de carga movimentada, velocidade do vento, umidade do material e fator de tamanho das partículas. Os resultados, referente aos meses de maio e agosto de 2017, em conjunto com os dados coletados em 16 de maio de 2017 pela técnica LIDAR, indicam que as emissões de material particulado pelos navios têm sua maior taxa de emissão para MP10 (moda grossa). Para as emissões fugitivas em operações com grãos, os resultados mostraram que as maiores emissões são provenientes de operações de soja em grãos. Em suporte às modelagens, foi utilizada a técnica LIDAR (Light Detection And Ranging) de sensoriamento remoto que utiliza radiação luminosa, e está baseada na emissão de um feixe de radiação eletromagnética que permite a obtenção de medidas a distância através da radiação retroespalhada. O pulso de luz emitido para a atmosfera interage com as partículas e moléculas que agem como espalhadoras. Os dados coletados são inseridos no programa Mathematica, e os valores obtidos inseridos na plataforma PYTON gerando gráficos. Os dados foram coletados em 16 de maio de 2017, com medidas em diferentes horários e identificada a possível presença de material particulado (MP) na região do porto. O porto de Santos, está localizado no centro do litoral do estado de São Paulo, é o maior porto da América Latina e um dos principais conflitos nessa região são as emissões de material particulado. A harbor is a dinamic organization that has critical áreas that contain pollutants sources such as insdutries in its surroundings, terrestrial modal (trucks and trains), operations with loading and unloading of agricultural bulk, ship flow, among others. This study used the model developed by the Current Methodologies in Preparing Mobile Source of the US Environmental Protection Agency (US EPA), which estimates the emission factors of particulate matter by ships considering the energy, characteristic and time of activity of each vessel, the propulsion load factor and the emission factor for each pollutant. To estimate fugitive emissions of particulate matter from port operations with corn, soybeans and soybean meal was used to model developed by Group Emissions Factor US EPA, which considers the volume of load handled, speed wind, humidity of the material and particle size factor. The results, referrinf to the months May and August of 2017, together with the data collected on May 16, 2017 by the LIDAR technique, indicate that emissions of particulate matter by ships have their highest emission rate for PM10 (coarse mode). For fugitive emissions of particulate matter in grain operations, the results showed that the highest emissions are from soybean grain operations. In support of modeling, It was used the LIDAR technology (Light Detection And Ranging) remote sensing which uses light radiation, and is based on the emission of an electromagnetic radiation beam that allows to obtain measurements at a distance by backscattering radiation. The pulse of light emitted into the atmosphere interacts with the particles and molecules that act as spreaders. The collected data are inserted into the Mathematica program and the values obtained are inserted into the PYTON platform generating graphs. The data were collected on May 16, 2017, with measurements at different times and identified the possible presence of particulate matter (PM) in the harbor region. The harbor of Santos, is located in the center of the coast of the state of São Paulo, is the bigger harbor in Latin America and one of the main conflicts in this region are emissions of particulate matter.

Conforme a população envelhece, aumenta o número de doenças crônicas não transmissíveis, dentre elas a insuficiência renal crônica, que em fase terminal leva o paciente a realizar uma terapia renal substitutiva, um dos métodos é na forma de hemodiálise, um tratamento que mantêm a vida, porém exige do paciente conviver com as restrições impostas pelas condições de saúde e tratamento. O objetivo deste trabalho é reconhecer as principais dificuldades vivenciadas pelos idosos em tratamento de hemodiálise no município de Cáceres-MT. Trata-se de um estudo descritivo, exploratório, de abordagem qualitativa, realizado com 16 idosos, entre os meses de agosto à novembro de 2020 para a entrevista utilizou-se um questionário semiestruturado contendo perguntas abertas. Entre os idosos entrevistados, 50% eram do sexo masculino e 50% feminino, com uma idade que variou de 60 a 81 anos. Após a análise das entrevistas obtivemos três categorias: percepções e vivências acerca do impacto da doença e o processo de aceitação do tratamento de hemodiálise; o idoso em tratamento de hemodiálise: principais dificuldades vivenciadas; e fé, família e equipe multiprofissional: enfrentamento da doença e apoio na adesão ao tratamento. Este estudo revelou que o idoso em hemodiálise convive com várias limitações e dificuldades, necessitando do total apoio dos familiares, sociedade, principalmente a equipe de enfermagem, ajudando-lhes na resolução das dificuldades encontradas, saber ouvi-los e compreendê-los em sua individualidade e magnitude, criando ações educativas para promover uma melhor qualidade de vida para que apesar das limitações estes idosos possam viver melhor.

OBJETIVO: Investigar a ação antibacteriana do própolis vermelhos 30%, do própolis verde 40% e do hipoclorito de sódio 2,5% com protocolos de irrigação em canais radiculares infectados. MÉTODOS: Durante 60 dias, vinte e quatro canais radiculares foram inoculados com E. faecalis. Em todos os grupos experimentais foi realizado o preparo do canal radicular e dois protocolos de irrigação – irrigação ultra-sônica passiva e irrigação convencional. Nos grupos 1, 3, 5 e 7 foi feito o preparo do canal radicular associado à irrigação convencional com própolis 30%, própolis 40%, hipoclorito de sódio 2,5% e água destilada, respectivamente. Nos grupos 2, 4, 6 e 8 foi feito o preparo do canal radicular associado à irrigação ultra-sônica passiva com as soluções descritas acima. Os grupos 9 e 10 foram os controles (negativos e positivos). Amostras dos canais radiculares foram coletadas e imersas em 7 mL de BHI, durante 48 horas, a 37ºC. O crescimento bacteriano foi analisado por turvação do meio de cultura. RESULTADOS: Ação antibacteriana foi observada com própolis vermelho 30% e hipoclorito de sódio 2,5% quando utilizado irrigação ultra-sônica passiva e irrigação convencional após 20 minutos, porém, após 72 horas as substâncias não foram efetivas. CONCLUSÃO: Os agentes de irrigação e os protocolos não foram efetivos para eliminar os microorganismos dos canais radiculares infectados

A urticária crónica espontânea é uma doença complexa, de etiologia mal-esclarecida, de diagnóstico fácil mas de abordagem terapêutica por vezes difícil. Tem um impacto significativo na qualidade de vida do doente e resulta em elevados custos diretos e indiretos. O diagnóstico da urticária crónica espontânea é essencialmente clínico, sendo recomendado um número limitado de exames complementares para diagnóstico diferencial e/ou para a investigação/exclusão de possível causa para a urticária crónica espontânea. Além do hemograma e da proteína C reativa, e/ou velocidade de sedimentação, os exames adicionais devem ser selecionados de acordo com critérios clínicos. O objetivo da terapêutica é o controlo clínico total da urticária crónica espontânea. A evolução deve ser documentada através do registo semanal do score de sintomas – Weekly Urticaria Activity Score (UAS7) –, assim como da avaliação de qualidade de vida. A abordagem terapêutica é baseada nos anti- histamínicos H1 (anti-H1) de segunda geração administrados de forma contínua nas doses aprovadas (primeira linha) e, na ausência de resposta clínica, até quatro vezes a dose diária aprovada (segunda linha). Os anti-H1 de primeira geração não são recomendados. Cerca de 30% dos doentes não ficam controlados com a terapêutica de segunda linha, pelo que é recomendado adicionar uma terapêutica de terceira linha. Das duas opções, omalizumab e ciclosporina, o omalizumab é a única opção com autorização de introdução no mercado para a urticária crónica espontânea, e possui melhor perfil de segurança, sendo assim recomendado preferencialmente. Em Portugal não existem recomendações nacionais aplicáveis à prática clínica. A elaboração destas recomendações é justificada pela necessidade de uniformização tanto da abordagem diagnóstica como da abordagem terapêutica dos doentes com urticária crónica espontânea em Portugal, e para a referenciação a centros especializados, nos casos mais graves.

This study investigates how travel agencies can achieve high customer loyalty through offline and online shopping experiences. The study testswhether different configurations of perceived value, customer satisfaction, perceived quality, and trust affect loyalty. The results froman online survey of a travel agency's 1974 offline and 1014 online customers provide the data. Using the fsQCA, the study finds that different combinations of these factors lead to higher customer loyalty. The findings show that trust is a sufficient condition for high customer loyalty only in the offline shopping context. In the online shopping context, travel agencies must combine trust with perceived value or with perceived quality to achieve high customer loyalty. Further, in the offline shopping context, the combination of perceived value and perceived quality leads to higher loyalty, while in the online shopping context agencies need to add customer satisfaction to this configuration. National Funds, funds this work through FCT (Fundação para a Ciência e a Tecnologia, Portugal info:eu-repo/semantics/publishedVersion

A label-free fiber optic biosensor based on a long period grating (LPG) and a basic optical interrogation scheme using off the shelf components is used for the detection of in-situ DNA hybridization. A new methodology is proposed for the determination of the spectral position of the LPG mode resonance. The experimental limit of detection obtained for the DNA was 62±2nM and the limit of quantification was 209±7nM. The sample specificity was experimentally demonstrated using DNA targets with different base mismatches relatively to the probe and was found that the system has a single base mismatch selectivity.

Introduction: The purpose of this clinical study was to compare the accuracy of working length (WL) determination using cone-beam computed tomography (CBCT), conventional periapical radiographies and electronic apex locator. Methods and Materials: This study was conducted during root canal treatment of 19 patients with a total of 30 single-rooted teeth diagnosed with apical periodontitis. After taking the initial parallel periapical radiographies, the initial file was advanced into the canal until the WL was detected by the apex locator. Subsequently, the WL was measured and WL radiographies were taken with the file set in the canal. Afterwards, CBCT images were acquired. These three measurements were tabulated and compared and the data were analyzed using the Friedman test. The level of significance was set at 0.05. Results: The mean values for WL determination by electronic apex locator, periapical radiograph and CBCT images were 22.25, 22.43 and 22.65, respectively which was not statistically significant (P>0.05). Conclusion: Working length determination using CBCT images was precise when compared to radiographic method and electronic apex locator.

Three indolenine-based barbiturate squaraine dyes were synthesized, characterized and subjected to photophysical studies, including their affinity with human serum albumin and bovine serum albumin as protein models in phosphate buffer solution. All dyes successfully interact with both proteins with high affinity binding constants. It was found that dyes with hydrophobic substituents had superior binding constants with both proteins. The fluorescence intensity of all dyes increased in the presence of both proteins which allowed the determination of detection and quantifications limits in the tens of nanomolar, using a protein concentration of 0–3.5 μM. Concerning to the study on the binding sites of the synthesized dyes using the warfarin and ibuprofen markers, the results of this study suggest that, one dye binds to both BSA binding sites while the two others dyes binds only to site I, and that all three dyes bind to both HSA binding sites.

The study of natural hybrid zones can illuminate aspects of lineage divergence and speciation in morphologically cryptic taxa. We studied a hybrid zone between two highly divergent but morphologically similar lineages (south-western and south-eastern) of the Iberian endemic Bosca's newt (Lissotriton boscai) in SW Iberia with a multilocus dataset (microsatellites, nuclear and mitochondrial genes). STRUCTURE and NEWHYBRIDS analyses retrieved few admixed individuals, which classified as backcrosses involving parental individuals of the south-western lineage. Our results show asymmetric introgression of mtDNA beyond the contact from this lineage into the south-eastern lineage. Analysis of nongeographic introgression patterns revealed asymmetries in the direction of introgression, but except for mtDNA, we did not find evidence for nonconcordant introgression patterns across nuclear loci. Analysis of a 150-km transect across the hybrid zone showed broadly coincident cline widths (ca. 3.2–27.9 km), and concordant cline centres across all markers, except for mtDNA that is displaced ca. 60 km northward. Results from ecological niche modelling show that the hybrid zone is in a climatically homogenous area with suitable habitat for the species, suggesting that contact between the two lineages is unlikely to occur further south as their distributions are currently separated by an extensive area of unfavourable habitat. Taken together, our findings suggest the genetic structure of this hybrid zone results from the interplay of historical (biogeographic) and population-level processes. The narrowness and coincidence of genetic clines can be explained by weak selection against hybrids and reflect a degree of reproductive isolation that is consistent with cryptic speciation., CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico), Grant/Award Number: 302892/2016-8; 232916/2013-2016; Fundação para a Ciência e a Tecnologia (FCT), Grant/Award Number: PTDC/BIABEC/105083/2008, SFRH/BPD/102966/2014 and UID/BIA/50027/2013; CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior ), Grant/Award Number: 88881.132701/2016-01; Spanish Severo Ochoa Program, Grant/Award Number: SEV-2012-0262; Program Operacional Factores de Competitividade (COMPETE), Grant/Award Number: POCI-01-0145-FEDER-006821.

Background Knee osteoarthritis tends to modify the kinematics and kinetics of the sit-to-stand task. However, it is not clear whether the different degrees of knee osteoarthritis differentiate regarding these aspects. The objective was to identify if the trunk flexion, lower limb kinetics, total support moment, and individual joint contributions to the total support moment during the sit-to-stand task are different between patients with mild and moderate knee osteoarthritis. Methods Sixty-two participants were grouped as follows: moderate knee osteoarthritis (n = 16), mild knee osteoarthritis (n = 25), and controls (n = 21). The participants performed a sit-to-stand task, which was analyzed using a 3D-motion system and a force plate. Findings The three phases of the sit-to-stand task were analyzed. During Phase1, the moderate osteoarthritis group decreased the total support moment (P = 0.012). During Phase2, the moderate osteoarthritis group showed higher trunk flexion (P = 0.023) and lower internal hip and knee extension moments (P ≤ 0.001 and P ≤ 0.040, respectively) when compared to controls. Also in Phase2, both the mild and moderate groups used lower total support moment (P = 0.019, and P ≤ 0.001, respectively). When compared to the controls and mild osteoarthritis group, those with moderate osteoarthritis presented higher hip joint contribution to the total support moment (P ≤ 0.001 and P = 0.006, respectively) as well as lower knee joint contribution (P ≤ 0.003 and P = 0,013, respectively). Interpretation Those with moderate osteoarthritis showed modified sit-to-stand movement pattern. While in Phase3 a higher contribution of the hip joint to the total support moment was observed, during previous phases the individuals were able to decrease the load on the knee without influencing the lower limb load distribution.

Freezing of Gait (FOG) is arguably the most disabling motor dysfunction in Parkinson’s Disease (PD), being pharmacological an unfeasible approach in the long-run, which justifies the emergence of non-pharmacological solutions. In fact, patients easily overcome FOG when it is provided a rhythmical temporal information (external cues), favoring a goal-directed gait (biofeedback systems). In this paper, it was intended to assess the tolerability and acceptability of a healthy group towards an augmented reality interface which aims to apply visual cues in temporal synchrony with heel-strike event by an augmented reality glasses. It is intended to be later assessed, the visual cues ability to facilitate motor activity in PD patients. The experimental setup comprised a group of 15 healthy subjects, being those instructed to perform a 30 m straight-walking along an unobstructed walkway, both in the presence of external cues and without any feedback applied. Questionnaires proven visual feedback to be perceived by the users with ease. Users reported interest and curiosity towards the system and proved to be keen to integrate the system into their daily life if they would ever face this disease.

Non-Newtonian nanofluids present outstanding features in terms of energy transfer and conductivity with high application in numerous areas. In this work, non-Newtonian nanofluids based on carbon dots (Cdots) functionalized with ionic liquids (ILs) are developed. The nanofluids are produced using a simple, single-step method where the raw materials for the Cdots synthesis are glucose and waste biomass (chitin from crab shells). The use of ILs as both reaction media and functionalization molecules allows for the development of a new class of nanofluids, where the ILs on the Cdots surface represent the base-fluid. Here, the well-known benign IL 1-butyl-3-methylimidazolium chloride ([Bmim]Cl) and a novel home-made IL (1-tosylate-3-methyl-imidazolium triflate) [Tmi][Trif] are used. The nanofluids obtained from both substrates show, apart from high conductivity and viscosity, light absorption, and good wettability, an appealing thermal sensitivity behavior. This thermal sensitivity is preserved even when applied as thin films on glass slides and can be boosted using the surface plasmon resonance effect. The results reported demonstrate that the new Cdots/IL-based nanofluids constitute a versatile and cost-effective route for achieving high-performance thermosensitive non-Newtonian sustainable nanofluids with tremendous potential for the energy coatings sector and heat transfer film systems., This research was funded by National Funds by Foundation for Science and Technology (FCT) and by FEDER funds through POCI-COMPETE 2020-Operational Programme Competitiveness and Internationalization in Axis I—Strengthening Research, Technological Development and Innovation (UID/QUI/00616/2013, UID/QUI/50006/2019, UID/ Multi/00709/2013, UID/QUI/00313/2019, UID/CTM/50025, POCI-01- 0145-FEDER-007491, POCI-01-0145-FEDER-007688, UID/CTM/50025, POCI-01-0145-FEDER-016884, PTDC/CTM-NAN/0956/2014, SAICT/ PAC/0032/2015, POCI-01-0145-FEDER-016422, and NORTE-01-0145- FEDER-030858). R.F.P.P. acknowledges FCT-MCTES for SFRH/ BPD/87759/2012 grant. E. Pereira (FCUP, Porto) is acknowledged for her assistance

Documento de apoio à Unidade Curricular de Estudos Práticos – Natação, do 1º ciclo em Ciências do Desporto da Universidade da Beira Interior

Exercise stress testing can have a central role in the assessment of cardiovascular disease. Contemporary data, however, has highlighted the added value of imaging modalities over the exercise electrocardiogram in the investigation of coronary artery disease. Given the physiological changes associated with exercise and the possibility to address other parameters such as rhythm changes and the chronotropic response, exercise stress testing with continuous electrocardiographic monitoring can still have an important place in contemporary clinical practice. We report the case of a complete atrioventricular block associated with exercise and discuss the current role of exercise stress testing with continuous electrocardiographic monitoring in this entity.

Objective To assess pulp oxygen saturation levels (SaO2) in maxillary central incisors after dental bleaching. Materials and Methods 80 participants (160 teeth) were randomly allocated to four groups: G1 In-office bleaching with two applications of 35% hydrogen peroxide (HP) (20 minutes), followed by at-home bleaching with 10% carbamide peroxide (CP) (2 hours/day for 16 days); G2 - Same protocol as G1, plus desensitizing toothpaste; G3 - In-office bleaching with 35% HP and one application of placebo gel (20 minutes), followed by at-home bleaching with 10% CP (2 hours/day for 16 days); and G4 - Same protocol as G3, plus desensitizing toothpaste. Pulp SaO2 levels were measured before (T0) and immediately after (T1) in-office bleaching; on the 5th (T2), 8th (T3), 12th (T4), and 16th days of at-home bleaching (T5); and on the 7th (T6) and 30th (T7) days. Mean (SD) pulp SaO2 levels were compared within groups by generalized estimating equations (GEE) and Student’s t-test (P

In this work, we report a new chemiluminescence system using bis-(2,4,6-trichlorophenyl) oxalate (TCPO) with hydrogen peroxide and luminol as fluorophore. The intense chemiluminescence reaction here described was fully investigated and it was determined that this fluorescent system has two strong light emissions at 440 and 490 nm, respectively. This new, user friendly, intense and striking light emission chemiluminescence system can be used as a very usefull tool for the design and construction of fluorescencent chemical sensors., This work received financial support from the European Union (FEDER funds through COMPETE) and National Funds (FCT - Fundação para a Ciência e a Tecnologia) through project UID/QUI/50006/2013.

Genetic studies on green sea turtles (Chelonia mydas) in the Eastern Atlantic have mostly focused on reproductive females, with limited information available regarding juveniles and foraging grounds. Improved understanding of genetic diversity and patterns of connectivity between nesting and foraging grounds is critical to identify management units and delineate suitable conservation strategies. Here we analyzed data from 11 microsatellite markers and sequences of the mitochondrial control region from both juveniles and females sampled in foraging and nesting aggregations around São Tomé and Príncipe islands, in the Gulf of Guinea, West Africa. Both nuclear and mtDNA data were congruent in showing that São Tomé and Príncipe's green turtles population exhibit high levels of genetic diversity, which are similar to those reported for other foraging aggregates in the Atlantic. Although signs of population substructure among foraging and nesting grounds of São Tomé and Príncipe islands were not apparent, our analysis based on mtDNA marker showed that both juvenile and adult turtles were genetically differentiated from other foraging and nesting Atlantic populations. The similar levels of genetic diversity found in both juveniles and females are consistent with the results from mixed stock analyses, which suggested that São Tomé and Príncipe's rookery is the primary source of juveniles to the local foraging aggregation. Taken these aspects in consideration, we argue that São Tomé and Príncipe green turtles show limited dispersal and should be considered an important management unit, and conservation actions in this archipelago must be implemented not only at the level of the rookery but should also include the foraging aggregations. Fundação para a Ciência e a Tecnologia - FCT info:eu-repo/semantics/acceptedVersion