Search

Your search keyword '"Harland, Richard"' showing total 822 results
Start Over Author "Harland, Richard"
822 results on '"Harland, Richard"'

221. Noncytopathic bovine viral diarrhea virus can persist in testicular tissue after vaccination of peri-pubertal bulls but prevents subsequent infection

Author

Givens, M. Daniel, primary, Riddell, Kay P., additional, Walz, Paul H., additional, Rhoades, Jim, additional, Harland, Richard, additional, Zhang, YiJing, additional, Galik, Patricia K., additional, Brodersen, Bruce W., additional, Cochran, Anna M., additional, Brock, Kenny V., additional, Carson, Robert L., additional, and Stringfellow, David A., additional

Published
2007
Full Text
View/download PDF

222. Using Epidemiology as an Aid in Feedlot Disease Management

Author

Ribble, Carl S., Van Donkersgoed, Joyce, Harland, Richard J., and Janzen, Eugene D.

Subjects
medicine.medical_specialty, Animal health, Computer science, animal diseases, fungi, food and beverages, Disease, Health records, medicine.disease, Epidemiology, Feedlot, medicine, Medical emergency, Disease management (health)
Abstract

The recent introduction of computers to the feedlot industry for monitoring animal health has markedly improved the efficiency with which we can use epidemiology as a means for developing new management techniques to prevent disease. With the computer we can keep accurate health records which can be analyzed rapidly by the attending veterinarian on a regular basis. We describe the kind of system we find useful for recording data on a computer in the feedlot. We also describe how those data can be summarized to help develop an epidemiological approach to disease management in the feedlot, using respiratory disease and hemophilosis as specific examples. Our experience is based upon working in medium-sized feedlots with capacities of several thousand to 15,000 or more head., American Association of Bovine Practitioners Proceedings of the Annual Conference, 1991

Published
1991
Full Text
View/download PDF

223. Wnt signaling in Xenopus embryos inhibits Bmp4 expression and activates neural development.

Author

Baker, Julie C., Harland, Richard M., and Beddington, Rosa S. P.

Subjects
*WNT protein genetics, *XENOPUS, *ECTODERM, *NEURAL development
Abstract

We report a new role for Wnt signaling in the vertebrate embryo: the induction of neural tissue from ectoderm. Early expression of mouse wnt8, Xwnt8, β-catenin, or dominant-negative GSK3 induces the expression of neural-specific markers and inhibits the expression of Bmp4 in Xenopus ectoderm. We show that Wnt8, but not the BMP antagonist Noggin, can inhibit Bmp4 expression at early gastrula stages. Furthermore, inhibition of β-catenin activity in the neural ectoderm of whole embryos by a truncated TCF results in a decrease in neural development. Therefore, we suggest that a cleavage-stage Wnt signal normally contributes to an early repression of Bmp4 on the dorsal side of the embryo and sensitizes the ectoderm to respond to neural inducing signals from the organizer. The Wnt targets Xnr3 and siamois have been shown previously to have neuralizing activity when overexpressed. However, antagonists of Wnt signaling, dnXwnt8 and Nxfrz8, inhibit Wnt-mediated Xnr3 and siamois induction, but not neural induction, suggesting an alternative mechanism for Bmp repression and neuralization. Conversely, dnTCF blocks both Wnt-mediated Xnr3 and neural induction, suggesting that both pathways require this transcription factor. [ABSTRACT FROM AUTHOR]

Published
2015

224. Whole transcriptome analysis with sequencing: methods, challenges and potential solutions.

Author

Jiang, Zhihua, Zhou, Xiang, Li, Rui, Michal, Jennifer, Zhang, Shuwen, Dodson, Michael, Zhang, Zhiwu, and Harland, Richard

Subjects
RNA sequencing, HUMAN genome, MOLECULAR biology, BIOMARKERS, RNA methylation, MESSENGER RNA, GENE targeting
Abstract

Whole transcriptome analysis plays an essential role in deciphering genome structure and function, identifying genetic networks underlying cellular, physiological, biochemical and biological systems and establishing molecular biomarkers that respond to diseases, pathogens and environmental challenges. Here, we review transcriptome analysis methods and technologies that have been used to conduct whole transcriptome shotgun sequencing or whole transcriptome tag/target sequencing analyses. We focus on how adaptors/linkers are added to both 5′ and 3′ ends of mRNA molecules for cloning or PCR amplification before sequencing. Challenges and potential solutions are also discussed. In brief, next generation sequencing platforms have accelerated releases of the large amounts of gene expression data. It is now time for the genome research community to assemble whole transcriptomes of all species and collect signature targets for each gene/transcript, and thus use known genes/transcripts to determine known transcriptomes directly in the near future. [ABSTRACT FROM AUTHOR]

Published
2015
Full Text
View/download PDF

225. The Alternative Splicing Regulator Tra2b Is Required for Somitogenesis and Regulates Splicing of an Inhibitory Wnt11b Isoform.

Author

Dichmann, Darwin S., Walentek, Peter, and Harland, Richard M.

Abstract

Summary Alternative splicing is pervasive in vertebrates, yet little is known about most isoforms or their regulation. transformer-2b (tra2b) encodes a splicing regulator whose endogenous function is poorly understood. Tra2b knockdown in Xenopus results in embryos with multiple defects, including defective somitogenesis. Using RNA sequencing, we identify 142 splice changes (mostly intron retention and exon skipping), 89% of which are not in current annotations. A previously undescribed isoform of wnt11b retains the last intron, resulting in a truncated ligand (Wnt11b-short). We show that this isoform acts as a dominant-negative ligand in cardiac gene induction and pronephric tubule formation. To determine the contribution of Wnt11b-short to the tra2b phenotype, we induce retention of intron 4 in wnt11b , which recapitulates the failure to form somites but not other tra2b morphant defects. This alternative splicing of a Wnt ligand adds intricacy to a complex signaling pathway and highlights intron retention as a regulatory mechanism. [ABSTRACT FROM AUTHOR]

Published
2015
Full Text
View/download PDF

226. Efficacy of parenteral antibiotics for disease prophylaxis in feedlot calves

Author

Harland, Richard J., Jim, G. Kee, Guichon, P. Timothy, Townsend, Hugh G.G., and Janzen, Eugene D.

Subjects
Articles
Abstract

Trimethoprim-sulfadoxine (TMPSDX) and two formulations of oxytetracycline (OTC) were examined for their prophylactic efficacy in feedlot calves when given by intramuscular injection on arrival at a large commercial feedlot. The study included 2,112 high-risk feeder calves that developed disease early in the feeding period. Both formulations of OTC reduced bovine respiratory disease morbidity during the first two weeks on feed and for the entire feeding period by 15-19% (p

Published
1991

232. Epidemiological features of calf mortality due to hemophilosis in a large feedlot

Author

Van Donkersgoed, Joyce, Janzen, Eugene D., and Harland, Richard J.

Subjects
animal diseases, Articles, bacterial infections and mycoses
Abstract

Hemophilosis was the most significant cause of mortality in fall-placed calves in a large feedlot in Saskatchewan, despite routine single immunization of calves with a commercial Haemophilus somnus bacterin on arrival. Common manifestations of fatal H. somnus infection were myocarditis and pleuritis; occasionally, thrombotic meningoencephalomyelitis (TME), peracute septicemia, and pneumonia were observed. Circumstantial evidence suggested that H. somnus may be a pathogen in polyarthritis. Death from pneumonia mainly occurred during the first five weeks in the feedlot. Death from myocarditis, pleuritis, TME, and septicemia, and euthanasia because of polyarthritis, occurred mainly after the third week in the feedlot.The median fatal disease onset (FDO) for pneumonia was day 12; for septicemia, day 17; for polyarthritis, day 18; for myocarditis and pleuritis, day 22; and for TME, day 29. Calves that died from myocarditis frequently were found dead in their "home" pen; however, 88% of these animals had been treated previously. Fifty-seven percent of the calves that died from pleuritis were never treated, and those that died from TME or septicemia were either never treated or died shortly after initial treatment.

Published
1990

233. Feedtrough dirt as a source of Clostridium botulinum type C intoxication in a group of farm horses

Author

Heath, Sebastian E., Bell, Roxy J., Chirino-Trejo, Manuel, Schuh, JoAnne C.L., and Harland, Richard J.

Subjects
Articles
Abstract

Four horses from the same farm developed clinical signs of botulism during the winter months; three of these horses died. One horse survived an initial attack and recovered over a three-week period, but died during a second attack. The horse that survived took six weeks to recover. Clinical and postmortem examination ruled out other causes of disease. Confirmation of the diagnosis was made by isolation of Clostridium botulinum type C toxin from the dirt in the bottom of an oak feedtrough used by all horses, and from the colonic contents of one of the horses that died. To our knowledge, this is the second case of C. botulinum type C intoxication reported in horses in North America. In both cases, soil and sand near aquatic environments were identified as the source of toxin.

Published
1990

243. Human dorsal tissue affecting factor (noggin) and nucleic acids encoding same

Author

Valenzuela, David M., Ip, Nancy Yuk-yu, Cudny, Henryk D., Yancopoulos, George D., Harland, Richard M., Smith, William C., Lamb, Teresa, Knecht, Anne, Valenzuela, David M., Ip, Nancy Yuk-yu, Cudny, Henryk D., Yancopoulos, George D., Harland, Richard M., Smith, William C., Lamb, Teresa, and Knecht, Anne

Abstract

Novel dorsal growth inducing factors, complexes including the factors, and DNA or RNA coding sequences for the factors are described.

Published
1998

Catalog

Books, media, physical & digital resources
See catalog results