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We study the nonlinear optomechanically induced transparency (OMIT) with gain and loss. We find that (i) for a single active cavity, significant enhancement can be achieved for the higher-order sidebands, including the transmission rate and the group delay; (ii) for active–passive-coupled cavities, hundreds of microsecond of optical delay or advance are attainable for the nonlinear sideband pulses in the parity-time-symmetric regime. The active higher-order OMIT effects, as firstly revealed here, open up the way to make a low-power optomechaical amplifier, which can amplify both the strength and group delay of not only the probe light but also its higher-order sidebands. [ABSTRACT FROM AUTHOR]

Background: Chimeric antigen receptor (CAR)-T cell therapy has achieved remarkable success in hematologic malignancies but faces significant limitations in gastrointestinal tumors due to the immunosuppressive tumor microenvironment (TME). Indoleamine 2,3-dioxygenase 1 (IDO1), a key enzyme in the TME, suppresses T cell efficacy by catalyzing tryptophan degradation to kynurenine (Kyn), leading to T cell exhaustion and reduced cytotoxicity. This study investigates the role of IDO1 inhibition in overcoming metabolic suppression by kynurenine and enhancing Claudin18.2 (CLDN18.2) CAR-T cell therapy in gastric and pancreatic adenocarcinoma models., Methods: We evaluated the impact of genetic knockdown and pharmacological inhibition of IDO1 (using epacadostat) on CAR-T cell functionality, including cytokine production and exhaustion marker expression. The effects of fludarabine and cyclophosphamide preconditioning on IDO1 expression, CAR-T cell infiltration, and antitumor activity was also examined. In vivo tumor models of gastric and pancreatic adenocarcinomas were used to assess the efficacy of combining IDO1 inhibition with CLDN18.2-CAR-T therapy., Results: IDO1 inhibition significantly enhanced CAR-T cell function by increasing cytokine production, reducing exhaustion markers by decreasing TOX expression and improving tumor cell lysis. Preconditioning with fludarabine and cyclophosphamide further suppressed IDO1 expression in the TME, facilitating enhanced CAR-T cell infiltration. In vivo studies demonstrated that combining IDO1 inhibition with CAR-T therapy led to robust tumor growth suppression and prolonged survival in gastric and pancreatic tumor models., Conclusions: Targeting IDO1 represents a promising strategy to overcome immunosuppressive barriers in gastrointestinal cancers, improving the efficacy of CLDN18.2-CAR-T therapy. These findings highlight the potential for integrating IDO1 inhibition into CAR-T treatment regimens to address resistance in treatment-refractory cancers., Competing Interests: Declarations. Ethics approval and consent to participate: All animal procedures were conducted in strict compliance with the protocols and guidelines established by the Institutional Animal Care and Use Committee (IACUC) for Shanghai model Organisms center. Consent for publication: All authors have approved the manuscript for submission. Competing interests: The authors declare that they have no competing interests., (© 2025. The Author(s).)

Heterogeneous single-metal-site catalysts (HSMSCs) have been recognized as promising materials due to the advantages of evenly distributed active metal sites, easy separation, and recyclability. Herein, we construct a single-site Ru catalyst on porous ionic polymers (Ru/PIPs-IL3) via electrostatic interactions, which is a practical and useful strategy for synthesizing HSMSCs. The strong electrostatic interactions (Δ H = -73.4 and -83.5 kJ/mol for Ru-PPh 4 and Ru-P(vBn)(vPh) 3 , respectively) between the quaternary phosphonium cations and oxygen anions of the cyclopentadienyl ligand in Ru species were both consolidated by DFT calculation and X-ray crystallography. The XPS, ATR-IR, HAADF-STEM, and EXAFS analyses and ion exchange experiment were employed to characterize the structure of Ru/PIPs-IL3. These results provide compelling evidence that PIPs-IL3 can interact with Shvo's catalyst in a manner analogous to that observed with quaternary phosphonium (PPh 4 Cl), thereby anchoring the Ru through electrostatic interactions. Such heterogeneous single-site Ru catalyst Ru/PIPs-IL3 exhibited a twofold activity compared to the homogeneous catalyst with a TON CO of up to 545 and an excellent 98.5% CO selectivity in the low-temperature reverse water-gas shift reaction.

Backscattering losses (BSL), arising from intrinsic imperfections or unavoidable external perturbations in optical resonators, can severely impact photonic devices. In single-photon systems, robust quantum correlations against BSL remain largely unexplored despite their significance for various applications. Here, we demonstrate that single-photon blockade (SPB), a purely quantum effect, can be preserved against BSL by introducing a nanotip near a Kerr nonlinear resonator with intrinsic defects. Without the tip, BSL disrupts SPB, but tuning the tip's position restores robustness even under strong BSL. Notably, quantum correlations emerge while the classical mean photon number remains suppressed due to the interplay between resonator nonlinearity and tip-induced optical coupling. Our findings highlight nanoscale engineering as a powerful tool to protect and harness fragile quantum correlations, paving the way for robust single-photon sources and backscattering-immune quantum devices.

Palladium-catalyzed asymmetric hydrogenation of tetrasubstituted olefin 4-substituted 3-alkoxycar-bonylfuran-2(5 H )-ones was developed for the construction of 1,2-contiguous stereogenters, giving the chiral trans- 4-substituted 3-alkoxycarbonylbutyrolactone derivatives with up to 95% of enantioselectivities. The asymmetric hydrogenation reaction could proceed smoothly at gram scale without any loss of reactivity and enantioselectivity. In addition, the synthetic utility of the chiral reductive products has been demonstrated through useful building blocks.

The hydrodynamics in the confluence area of rivers are typically governed by the geometrical configuration of the surrounding landforms, the momentum and discharge ratios of the converging watercourses, and the morphological features of the riverbed. Recently, it has been discovered that stratification effects resulting from disparities in temperature, salinity, sediment concentration can also have significant impact on the downstream hydrodynamics of the confluence area. In this study, the impact of stratification induced by sediment concentration on fluid dynamics and pollutant mixing in the Yellow - Fen River confluence area is studied by using a 3D numerical model. The distribution of the depth-averaged field and secondary flow are analyzed. The findings revealed that the alterations in density caused by sediment concentration had effect on the flow dynamics across both horizontal and vertical dimensions of the confluence area. These alterations encompassed flow magnitude, shear layer, low velocity zone, cross-section vortex, and secondary flow. When the sediment concentration in the main stream was high, a noticeable stratification phenomenon emerged, whereby the tributary pollutants rode above the main stream during transport and mixing. Under these conditions, the pollutant mixing process accelerated, resulting in a shortened mixing distance. This acceleration can be attributed to the intensified secondary flow in the cross-section, brought about by the stratification effect. The findings from this study enhance our understanding of pollutant mixing patterns in river confluence areas., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024. Published by Elsevier B.V.)

Dysfunctional NF-κB signaling is critically involved in inflammatory bowel disease (IBD). We investigated the mechanism by which RIPK1 and TRADD, two key mediators of NF-κB signaling, in mediating intestinal pathology using TAK1 IEC deficient model. We show that phosphorylation of TRADD by TAK1 modulates RIPK1-dependent apoptosis. TRADD and RIPK1 act cooperatively to mediate cell death regulated by TNF and TLR signaling. We demonstrate the pathological evolution from RIPK1-dependent ileitis to RIPK1- and TRADD-co-dependent colitis in TAK1 IEC deficient condition. Combined RIPK1 inhibition and TRADD knockout completely protect against intestinal pathology and lethality in TAK1 IEC KO mice. Furthermore, we identify distinctive microbiota dysbiosis biomarkers for RIPK1-dependent ileitis and TRADD-dependent colitis. These findings reveal the cooperation between RIPK1 and TRADD in mediating cell death and inflammation in IBD with NF-κB deficiency and suggest the possibility of combined inhibition of RIPK1 kinase and TRADD as a new therapeutic strategy for IBD., Competing Interests: Competing interests: The authors declare no competing interests., (© 2025. The Author(s).)

Background: Lactate-to-albumin ratio (LAR) is an emergency predictive indicator of sepsis-related mortality. An elevated LAR is associated with poor outcomes in critically ill patients. However, its predictive value for acute kidney injury (AKI) in patients with sepsis remains unclear. Therefore, this study aimed to investigate the relationship between LAR and AKI in patients with sepsis., Methods: The study population was derived from the Medical Information Mart for Intensive Care-IV (2.0) database and stratified into quartiles based on the LAR. The primary endpoint was the occurrence of AKI. The secondary endpoints were the use of renal replacement therapy (RRT) and in-hospital mortality. Kaplan-Meier survival analysis and Cox proportional hazards models were used to assess the association between the LAR index and risk of AKI in patients with sepsis., Results: In this study, 5,222 patients with sepsis were included, of whom 3,029 were male (58%). Kaplan-Meier survival analysis demonstrated significant differences in the cumulative incidence of AKI and cumulative usage rate of RRT among patients with sepsis based on the quartiles of the LAR index. Additionally, Cox proportional hazards analysis adjusted for confounding factors showed a significant association between the LAR index and incidence of AKI in patients with sepsis., Conclusion: Our study indicated that a high LAR index can serve as an independent predictor of AKI in patients with sepsis., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2025 Hua, Ding, Jing, Xie, Wu, Wu and Lan.)

The synthesis of high-purity gold nano bipyramids (Au NBPs) with a narrow size distribution and tunable plasmon resonances is of great significance for plasmon resonance-related applications. However, the synthesis Au NBP approach involves multiple steps with many parameters that can affect the purity of the final product. In this work, we were devoted to studying the effect of the molar ratio between hexadecyltrimethylammonium chloride (CTAC) and sodium citrate tribasic dihydrate (CiNa 3 ) on the seed formation stage. The results showed that the yield of Au NBP product has dramatically increased with the seed solution made from the molar ratio of CTAC:CiNa 3 at 21:1. Furthermore, using this optimal seed, we can efficiently synthesize Au NBPs with various sizes by adjusting the concentration of the seed but keeping the rest of the parameters constant. In this study, the longitudinal localized surface plasmon resonances (LSPRs) of Au NBPs exhibit tunability beyond 450 nm across the visible and near-infrared regions from 774 to 1224 nm. We were able to successfully fine-tune the LSPRs of Au NBPs in the spectral region to become resonant with the excitation wavelengths of an 808 nm near-infrared (NIR) laser. The photothermal activities of Au NBPs were studied under 808 nm laser irradiation at ambient conditions. The present work demonstrates a paradigm for the synthesis of Au NBPs with tunable LSPRs in a precise and controllable manner, achieved by examining the surfactant ratios in the formation of penta-twinned seeds., Competing Interests: The authors declare no competing financial interest., (© 2025 The Authors. Published by American Chemical Society.)

Halide perovskites (HPs), emerging as a noteworthy class of semiconductors, hold great promise for an array of optoelectronic applications, including anti-counterfeiting, light-emitting diodes (LEDs), solar cells (SCs), and photodetectors, primarily due to their large absorption cross section, high fluorescence efficiency, tunable emission spectrum within the visible region, and high tolerance for lattice defects, as well as their adaptability for solution-based fabrication processes. Unlike luminescent HPs with band-edge emission, trivalent rare-earth (RE) ions typically emit low-energy light through intra-4f optical transitions, characterized by narrow emission spectra and long emission lifetimes. When fused, the cooperative interactions between HPs and REs endow the resulting binary composites not only with optoelectronic properties inherited from their parent materials but also introduce new attributes unattainable by either component alone. This review begins with the fundamental optoelectronic characteristics of HPs and REs, followed by a particular focus on the impact of REs on the electronic structures of HPs and the associated energy transfer processes. The advanced synthesis methods utilized to prepare HPs, RE-doped compounds, and their binary composites are overviewed. Furthermore, potential applications are summarized across diverse domains, including high-fidelity anticounterfeiting, bioimaging, LEDs, photovoltaics, photodetection, and photocatalysis, and conclude with remaining challenges and future research prospects., (© 2025 Wiley‐VCH GmbH.)

Introduction: Maternal smoking during pregnancy is associated with placental DNA methylation and RNA expression, offspring DNA methylation, and affects the decline of mature neurons and the prenatal human brain development trajectory., Methods: This study is a secondary analysis of data from the National Health and Nutrition Examination Survey (NHANES) spanning 2003 to 2008, comprising 10111 children and adolescents. Inclusion criteria required participants to have complete questionnaire responses regarding maternal smoking during pregnancy and receipt of special education or early intervention services. The risk of developmental disabilities was assessed using a multifactor logistic regression model., Results: In the cohort of 10111 children and adolescents, 727 (7.2%) received special education or early intervention services. Of these participants, 1504 (14.9%) were exposed to maternal smoking during pregnancy. The prevalence of maternal smoking was higher (12.3%) in the group receiving special education or early intervention compared to those who did not (6.3%). After adjusting for other relevant factors in a multifactorial logistic regression model, maternal smoking during pregnancy was significantly associated with an increased likelihood of requiring special education or early intervention services (adjusted odds ratio, AOR=1.51; 95% CI: 1.24-1.83, p<0.001)., Conclusions: This cross-sectional analysis found an association between maternal smoking during pregnancy and the need for special education or early intervention services among US children and adolescents, after adjusting for confounding variables. Our findings suggest that maternal smoking during pregnancy may increase the odds of developmental disabilities., Competing Interests: The authors have completed and submitted the ICMJE Form for Disclosure of Potential Conflicts of Interest and none was reported., (© 2025 Miao H. et al.)

Affinity capture (AC) combined with mass spectrometry (MS)-based proteomics is highly utilized throughout the drug discovery pipeline to determine small-molecule target selectivity and engagement. However, the tedious sample preparation steps and time-consuming MS acquisition process have limited its use in a high-throughput format. Here, we report an automated workflow employing biotinylated probes and streptavidin magnetic beads for small-molecule target enrichment in the 96-well plate format, ending with direct sampling from EvoSep Solid Phase Extraction tips for liquid chromatography (LC)-tandem mass spectrometry (MS/MS) analysis. The streamlined process significantly reduced both the overall and hands-on time needed for sample preparation. Additionally, we developed a data-independent acquisition-mass spectrometry (DIA-MS) method to establish an efficient label-free quantitative chemical proteomic kinome profiling workflow. DIA-MS yielded a coverage of ∼380 kinases, a > 60% increase compared to using a data-dependent acquisition (DDA)-MS method, and provided reproducible target profiling of the kinase inhibitor dasatinib. We further showcased the applicability of this AC-MS workflow for assessing the selectivity of two clinical-stage CDK9 inhibitors against ∼250 probe-enriched kinases. Our study here provides a roadmap for efficient target engagement and selectivity profiling in native cell or tissue lysates using AC-MS.

Classical swine fever (CSF) caused by classical swine fever virus (CSFV) has resulted in severe losses to the pig industry worldwide. CSFV non-structural protein 4B (NS4B) plays a crucial role in CSFV replication and pathogenicity. However, the function of NS4B is still limited during CSFV infection. In this study, the RNA-seq was used to investigate differentially expressed genes (DEGs) in 3D4/21 cells expressing CSFV NS4B. 4397 DEGs were identified in 3D4/21 cells expressing NS4B compared to cells expressing the empty vector (NC). Twelve DEGs were selected and further verified by RT-qPCR. Enrichment analyses of GO annotations and KEGG pathways revealed that these DEGs were associated with endocytosis, autophagy, cell adhesion, transport, immune response, apoptosis and so on. The expression of endocytosis-related genes, including CAV1/2, CAVIN2, Rab1B, CHMP2B/4C, VPS35, SNX2, Rab11B, CHMP6, MVB12B and VPS28, were found to be regulated. In addition, some genes associated with host immune defense, such as USP15, DHX29, DDX3, RIG-I and MDA5, were downregulated and the genes associated with host autophagy, such as WIPI2, ATG16L2, SMCR8, RPTOR and MLST8, were upregulated. Therefore, CSFV NS4B involved in virus invasion and intracellular trafficking, the induction of autophagy and the inhibition of antiviral response. Taken together, this study provides useful information for further understanding the function of NS4B during CSFV infection., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2025 Dong, Lv, Song, Lv, Xu, Jing, Peng, Song and Guo.)

Heavy-legged robots (HLRs), integral to optimizing efficiency in manufacturing and transportation, rely on advanced active servo fault diagnosis and fault-tolerant control (FTC) mechanisms. This study presents an FTC framework with active fault status identification, fault tolerance capability assessment, and model uncertainty handling. A key contribution is the introduction of an active servo fault state estimator (ASFSE), which enables real-time monitoring of servo status by comparing residual differences between servo and controller outputs. The system's tolerance capability interval (TCI) is tied to the servo state, with the dual-line particle filters (DPF) algorithm predicting when the HLR exceeds the TCI under faults. Subsequently, a target trajectory modifier (TTM) and fixed-time backstepping controller (FTBC) are proposed. The TTM promptly adjusts the trajectory when the HLR surpasses the TCI, while the FTBC ensures fixed-time convergence based on the predicted failure time for precise trajectory tracking. As the HLR approaches its fault tolerance limits, the TTM and FTBC ensure a smooth stop, thus mitigating equipment damage caused by servo faults. Mathematical stability proof and simulation validations confirm the effectiveness of the FTC framework., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2025 International Society of Automation. Published by Elsevier Ltd. All rights reserved.)

Lysozymes are crucial enzymes involved in the innate immune response against bacterial pathogens. In this study, we identified and characterized a goose-type lysozyme gene (SsLyG) from the black rockfish Sebastes schlegelii, an economically important aquaculture species. The deduced amino acid sequence of SsLyG contains 495 residues, which inculded a signal peptide, an immunoglobulin domain, and a goose egg-white lysozyme (GEWL) domain. Tissue expression analysis revealed the highest SsLyG levels in blood, and its transcription was significantly upregulated in the spleen and kidney upon bacterial and polyI:C challenges. Recombinant SsLyGE (rSsLyGE) exhibited lytic activity against Micrococcus lysodeikticus and concentration-dependent binding ability to Staphylococcus aureus and Micrococcus luteus. Furthermore, rSsLyGE promoted peripheral blood lymphocyte proliferation, enhanced macrophage respiratory burst activity, and increased reactive oxygen species production. RNA interference-mediated knockdown of SsLyG resulted in higher bacterial loads in the liver and spleen after Listonella anguillarum challenge, suggesting its role in early antibacterial defense. Collectively, these findings provide insights into the immune function of SsLyG and its potential application in developing antimicrobial strategies for aquaculture., Competing Interests: Declaration of competing interest The authors declare that they have no competing interest., (Copyright © 2024 Elsevier Inc. All rights reserved.)

Porcine reproductive and respiratory syndrome virus (PRRSV) is a highly contagious swine pathogen, causing respiratory problems in piglets and reproductive failure in sows. Palmitoylation, catalyzed by zinc finger Asp-His-His-Cys (ZDHHC) domain-containing palmitoyl acyltransferases, plays intricate roles in virus infection. However, whether palmitoylation regulates PRRSV replication is incompletely understood. Here, we report that inhibition of palmitoylation by 2-bromo palmitate (2-BP) promotes PRRSV multiplication. ZDHHC3 is identified as the key palmitoyl transferase regulating PRRSV replication in PAMs infection. Mechanistically, ZDHHC3 catalyzes nucleocapsid (N) protein palmitoylation at cysteine 90. This modification prevents the Nsp9-N protein interaction and subsequent viral RNA synthesis. Furthermore, LYPLA1 de-palmitoylates N protein, thus counteracting the ZDHHC3's activity on PRRSV replication. Meanwhile, the administration of small-molecule inhibitor ML348 targeting LYPLA1 could hinder PRRSV-2 replication. In summary, our results underscore the critical role of reversible palmitoylation in PRRSV replication. These findings might provide potential new anti-PRRSV strategies., Competing Interests: Declaration of Competing Interest The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2025 Elsevier B.V. All rights reserved.)

Stretchable electronic devices that conduct strain-related electronic performances have drawn extensive attention, functioning as mechanical sensors, actuators, and stretchable conductors. Although strain-insensitive or strain-responsive nature is well-achieved separately, it remains challenging to combine these two characteristics in one single device, which will offer versatile adaptability in various working situations. Herein, a hybrid material with sign-switchable Poisson's ratio (SSPR) is developed by combining a phase-change gel based reentrantreentrant honeycomb pattern and a polydimethylsiloxane film. The phase-change gel featuring thermally-regulated Young's modulus enables the hybrid material to switch between negative and positive Poisson's ratios. After integrating with a pre-stretched silver nanowires film, the obtained stretchable device performs bimodal strain-to-electrical signal transducing (Bi-SET) functions, in which the SSPR-dominated strain-resistance response switches between strain-dependent and strain-insensitive behaviors. As a proof of concept, a mode-switchable grasping system is constructed using a Bi-SET device-based controller, enabling the adaptation of grasping behaviors to various target objects., (© 2024 Wiley‐VCH GmbH.)

Developing an eco-friendly and effective approach for preparing N, S co-doped hierarchical porous carbons (NSHPC) for capacitive deionization (CDI) is a huge task for desalination. Herein, NSHPC SKK with interconnected hierarchical pore structures, manufactured via self-activation/co-activation of sodium lignosulfonate (SLS) encapsulation using KNO 3 -KHCO 3 activators, inducing N, S co-doping. Different from NSHPC S and NSHPC SK , NSHPC SKK exhibits the highest specific surface area (S BET , 2264.67 m 2 /g) and a unique hierarchical pore structure (mesoporous volume/pore volume (V meso / V pore ), 0.65). Small-angle X-ray scattering (SAXS) and scanning electron microscopy (SEM) both reveal the complex interconnected pore structure of NSHPC SKK . Regional Raman imaging conjugated with XPS reveals the presence of extensively distributed N, S co-doped defect structures, providing NSHPC SKK with excellent wettability and electrochemical performance. DFT calculations indicate that the N, S co-doping at the defect sites depicts excellent adsorption capability. Eventually, NSHPC SKK acquired an impressive salt adsorption capacity (SAC) of 20.5 mg/g and the highest average salt adsorption rate (ASAR) of 12.1 mg/g/min, indicating its superior desalting performance. In-situ Raman spectroscopy confirms NSHPC SKK 's rapid ion regeneration mechanism. The research introduces a span-new NSHPC synthesis strategy for fabricating advanced NSHPC with rapid desalination response for upgrading CDI desalination., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Inc. All rights reserved.)

Objective: The aim of the present study was to explore the relationship between the size of cesarean scar diverticulum (CSD) measured on preoperative magnetic resonance imaging (MRI) and adverse events during dilatation and curettage (D&C) procedure in patients with cesarean scar pregnancy (CSP)., Methods: The MRI of 197 CSP patients from October 2019 to August 2023 were retrospectively reviewed. The volume, area, and depth of CSD, residual myometrium thickness (RMT), and gestational sac diameter were recorded and tested for correlation with intraoperative estimated blood loss (EBL), and operation time and for any association with the intraoperative adverse events (intraoperative massive hemorrhage [39 cases] and D&C procedure failure [15 cases]). The Spearman test was used to characterize the correlation between the five MRI variables and both the EBL and operation time. The correlation between the five MRI variables and intraoperative adverse events was evaluated with student's t test and Mann-Whitney U test. Diagnostic power of the MRI variables was evaluated by the area under receiver operating characteristic curve (AUC)., Results: The volume, area, and depth of CSD and gestational sac diameter were positively correlated with both EBL and operation time, with the CSD volume having the highest correlation with them (r = 0.543 and 0.461, respectively). Conversely, the RMT displayed a negative correlation with the EBL and operation time. All five MRI variables were significantly associated with both intraoperative massive hemorrhage and D&C failure (all P < 0.001). The CSD volume demonstrated the highest AUC for diagnosing intraoperative massive hemorrhage and D&C failure at 0.893 (95% CI: 0.82-0.92) and 0.901 (95% CI: 0.85-0.94), respectively. The optimal cutoff values for CSD volume in predicting massive hemorrhage and D&C failure were determined to be 5.41 and 8.92 cm 3 , respectively, with corresponding sensitivities/specificities of 92.31/74.68 and 93.33/82.42, respectively., Conclusion: Quantifying the size of CSD based on preoperative MRI could aid in evaluating risk during D&C in CSP patients, with CSD volume possessing higher diagnostic efficacy than the other four MRI indicators., (© 2024 International Federation of Gynecology and Obstetrics.)

DELAY OF GERMINATION1 (DOG1) is a critical regulator of seed dormancy and seed thermoinhibition. However, how DOG1 expression is regulated by post-translational modifications and how seeds transmit the high-temperature signal to DOG1 remain largely unknown. ALFIN1-like 6/7 (AL6/7) was previously found to repress DOG1 expression during seed imbibition. Here, we found that AL6/7 represses seed dormancy partly by downregulating DOG1 expression. AtSIZ1, a SUMO E3 ligase, interacts with AL6 and mediates its SUMOylation mainly at the lysine 181 residue. SIZ1-mediated SUMOylation of AL6 is required for repression of DOG1 transcription and seed dormancy. SUMOylation of AL6 is required for its association with the DOG1 locus and protects it from ubiquitination and subsequent 26S proteasome-mediated protein degradation. High temperatures decrease SUMOylation levels of AL6, resulting in downregulation of AL6 protein levels and an increase in DOG1 transcription, which consequently causes reduced seed germination. Taken together, these results demonstrate that reversible SUMOylation of AL6 fine-tunes DOG1 expression, which is required for precise establishment of seed dormancy and inhibition of seed germination under high-temperature conditions in Arabidopsis thaliana., (© 2024 The Author(s). New Phytologist © 2024 New Phytologist Foundation.)

Chromium oxychloride (CrOCl), a van der Waals antiferromagnetic insulator, has attracted significant interest in 2D optoelectronic, ferromagnetic, and quantum devices. However, the bottom-up preparation of 2D CrOCl remains challenging, limiting its property exploration and device application. Herein, the controllable synthesis of 2D CrOCl crystals by chemical vapor deposition is demonstrated. The combination reaction of precursors together with the space-confined growth strategy, providing stable and stoichiometric growth conditions, enable a robust synthesis of high-crystallinity CrOCl nanosheets with regular rhombus-like morphology and uniform thickness. By tuning the growth temperature from 675 to 800 °C, the thickness of CrOCl nanosheets can be continuously modulated from 10.2 to 30.8 nm, with the domain size increasing from 16.9 to 25.5 µm. The as-grown CrOCl nanosheets exhibit significant structural/optical anisotropy, ultrahigh insulativity, and superior air stability. Furthermore, a MoS 2 /CrOCl heterostructure with single-mirror symmetry stacking and ultrastrong interfacial coupling is built to realize interfacial symmetry breaking, a novel interface phenomenon that converts MoS 2 from isotropy to anisotropy. Consequently, the MoS 2 /CrOCl heterostructure device achieves polarization-sensitive photodetection and bulk photovoltaic effect, which are nonexistent in high-symmetry 2D materials. This work paves the way for the future exploration of CrOCl-based 2D physics and devices via symmetry engineering., (© 2024 Wiley‐VCH GmbH.)

Porcine reproductive and respiratory syndrome virus (PRRSV) infection causes reproductive failure and respiratory distress and is a serious threat to the swine industry, given its continuous and rapid emergence. The knowledge of viral-host interaction could facilitate anti-PRRSV drug development. HnRNP A1 is an abundantly expressed protein which associates with RNA metabolic processes and plays multifarious roles during the reproduction cycle of multiple viruses. However, the function of porcine HnRNP A1 in PRRSV-2 replication is still unknown. Herein, HnRNP A1 was identified as a nucleocapsid (N)-binding protein for PRRSV-2. Overexpression of porcine HnRNP A1 promoted the expression of viral RNA, and viral proteins, corresponding to enhanced virus titers. While deletion of the UP1 domain abolished the HnRNP A1-mediated enhancement of PRRSV-2 replication. In addition, HnRNP A1-silencing confirmed its pro-viral effect on PRRSV-2 infectivity in porcine alveolar macrophages (PAMs). RNA pull-down and RNA immunoprecipitation verification confirmed that the UP1 domain is important for the recognition of the guanine-rich sequence (GRS) in PRRSV-2 negative RNA. Eventually, supplementation with TMPyP4, a G4 ligand, efficiently provokes the release of HnRNP A1 from GRS, thereby limiting PRRSV-2 replication. Together, these findings help to inform the mechanism by which HnRNP A1 accelerates PRRSV-2 replication, and facilitate antiviral drug design., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Inc. All rights reserved.)

What Is Already Known About This Topic?: Scrub typhus is an acute infectious disease caused by Orientia tsutsugamushi that is transmitted primarily through the bite of infected chigger mite larvae. The disease prevalence is closely associated with environments characterized by high moisture levels and abundant vegetation., What Is Added by This Report?: This study documents the first confirmed case of scrub typhus in Ningxia Hui Autonomous Region, China. The patient presented with characteristic clinical manifestations, including abnormal biochemical indicators and positive serum-specific IgM antibodies against scrub typhus. Epidemiological evidence suggests local acquisition of the disease., What Are the Implications for Public Health Practice?: Enhanced surveillance and preventive measures for scrub typhus are essential in this region, particularly for individuals residing in or visiting areas where the disease may be endemic., Competing Interests: No conflicts of interest., (Copyright © 2025 by Chinese Center for Disease Control and Prevention.)

In this study, we investigated the F1 antibody against Yersinia pestis in the sera of mother-neonate shepherd dog pairs in the Marmota himalayana plague focus of the Altun-Qilian Mountains, Qinghai-Tibetan Plateau, China. Seropositive shepherd dogs lived in plague-endemic regions, where marmots were infected with Y. pestis , whereas seronegative dogs lived in non-endemic regions. The neonatal F1 antibody titers positively correlated with the maternal titers within 3 months after birth, and the neonatal titers were similar to or slightly lower than the maternal titers. In the absence of reinfection, antibodies in the neonates were obtained from their mothers; titers decreased with age and disappeared after 3 months. Mean tumor necrosis factor (TNF)-α, interleukin (IL)-6, IL-2, IL-10, and nerve growth factor (NGF)-β were higher in the mothers than in neonates. Maternal TNF-α, IL-10, vascular endothelial growth factor (VEGF)-A, and NGF-β and neonatal monocyte chemoattractant factor (MCP)-1 and VEGF-A were positively correlated with F1 antibody titers. Our results reveal continuing vertical transmission of F1 antibodies between mother dogs and their offspring and cytokine signatures under plague., Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© 2025 The Author(s).)

Ethnopharmacological Relevance: The dried larvae of Holotrichia diomphalia Bates, named Qi Cao, is a traditional Chinese medicine treat for liver diseases and arthritis. Polysaccharides is a principal component in Qi Cao, which exhibiting antioxidant and anti-inflammatory effects. However, the structural characteristics and underlying mechanisms of the polysaccharides remain inadequately elucidated., Aim of the Study: To analyze the primary structure and elucidate the molecular anti-inflammatory mechanisms of the active polysaccharide in Qi Cao., Materials and Methods: The total polysaccharide was extracted by water extraction and alcohol precipitation, and further isolated and purified by DEAE Sephadex A-25 column and Sephadex G-100 column. The anti-inflammatory properties of four major fractions (HDPS-1, HDPS-2, HDPS-3, HDPS-4) and the pure homogeneous polysaccharides (HDPS-1I and HDPS-1II) were assessed using a RAW 264.7 cell model induced by lipopolysaccharide (LPS), and HDPS-1II was identified as the polysaccharide exhibiting significant anti-inflammatory activity in Qi Cao. The structural characteristics of HDPS-1II were subsequently analyzed using high-performance size-exclusion chromatography (HPSEC), fourier-transform infrared spectroscopy (FT-IR), gas chromatography-mass spectrometry (GC-MS), and nuclear magnetic resonance (NMR) spectroscopy. The TLR4, NF-κB, COX-2 and iNOS expressions were determined by Western blot analysis to investigate the anti-inflammatory mechanism of HDPS-1II in vitro. Finally, the in vivo anti-inflammatory activity of HDPS-1II were evaluated by measuring the serum levels of pro-inflammatory factors, inflammatory cell infiltration and organelle damage in the lung tissues of sepsis model mice., Results: A homogeneous polysaccharide (HDPS-1II) with molecular weight of 1.7 × 10 4  Da was isolated from Holotrichia diomphalia Bates. HDPS-1II contains a backbone of α-T-Glcp-(1 → 6)-α-Glcp-(1 → 4)-α-Galp-(1 → 4)-α-Galp-(1 → 6)-α-Galp-(1 → 3)-α-Galp-(1 → . It inhibited activation of the TLR4/NF-κB signaling and reduced pro-inflammatory factors and NO in LPS-stimulated macrophage. Moreover, HDPS-1II increased the survival rate, inhibited inflammatory cells infiltration, and ameliorated the lung tissue damage in septic mice., Conclusions: HDPS-1II exhibits anti-inflammatory effects in vitro and in vivo, which is the active polysaccharide components of the anti-inflammatory activity of Qi Cao., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Purpose: Breast cancer patients experience more gastrointestinal symptoms during chemotherapy than during the survival period post-treatment. This can interfere with daily dietary intake and increase the risk of malnutrition. This study aimed to evaluate the level and classification of dietary self-management behavior and the influence of self-care self-efficacy, outcome expectations, treatment self-regulation and perceived social support guided by "Integrated theory of Health Behavior Change"., Methods: A multicenter cross-sectional study was conducted. The assessment tools included self-designed demographic and clinical instruments, Dietary Management Behavior Questionnaire, Strategies Used by People to Promote Health, Consideration of Future Consequences Scale-Food, the Treatment Self-regulation Questionnaire and Perceived Social Support Scale., Results: A total of 760 samples were analysed. The best-fitting model of the 4 classes was determined, with the lowest-to-highest scores as follows: Class 1-poor behavior group (17.84%), Class 2-medium behavior group (28.26%), Class 3-good behavior group (22.67%) and Class 4-excellent behavior group (31.23%). In the control group, being underweight (OR = 0.131), having no spouse (OR = 0.235), having a lower educational level (OR = 0.147-0.384), being premenopausal (OR = 0.220-0.255), having immediate consequence considerations (OR = 0.866-0.909) and receiving other support (OR = 0.830) were risk factors (all P < 0.05)., Conclusions: The performance of dietary self-management behavior needs to be improved. This study may provide a reference for determining key intervention populations that are underweight during premenopause, have no spouse and have lower education. Specific intervention content, including future consequence consideration, positive attitudes, decision-making, autonomous motivation, introjected regulation and friend support, should be added to improve the dietary practices during chemotherapy precisely., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or relationships that could have appeared to influence the work reported in this paper., (Copyright © 2025 Elsevier Ltd. All rights reserved.)

Background: HMPL-306 has equally high inhibitory activity against mutated isocitrate dehydrogenases 1 and 2 (mIDH1/2)., Methods: This first-in-human, phase 1 dose-escalation/dose-expansion study (this study was registered at ClinicalTrials.gov: NCT04272957) enrolled patients with relapsed/refractory (R/R) acute myeloid leukemia (AML) harboring mIDH1 and/or mIDH2. Patients received 25-250 mg of HMPL-306 orally once daily (QD) in a 28-day treatment cycle. Primary objectives were safety, tolerability, and recommended phase 2 dose (RP2D), and the secondary objective was preliminary efficacy., Findings: A total of 76 patients were enrolled. No dose-limiting toxicities were observed, and the maximum tolerated dose was not reached. RP2D was 250 mg QD for cycle 1 and 150 mg QD from cycle 2 onward. Common (≥10%) grade ≥3 treatment-related adverse events included platelet count decreased, anemia, neutrophil count decreased, and white blood cell count decreased. In patients who received 150 mg, 250 mg, or the RP2D (N = 59), rates of complete remission (CR)+CR with partial hematologic recovery were 34.6% and 36.4% in the mIDH1 (n = 26) and mIDH2 (n = 33) subgroups, respectively, and among these, CR with minimal residual disease negative rates were 77.8% and 50.0%, respectively. The median overall survival was 13.4 months in patients with mIDH1 and 13.1 months in patients with mIDH2., Conclusions: HMPL-306 showed an acceptable safety profile and promising preliminary efficacy. A phase 3, randomized study of HMPL-306 in R/R AML (this study was registered at ClinicalTrials.gov: NCT06387069) has been initiated., Funding: HUTCHMED Limited, National Key Research and Development Program of China, National Natural Science Foundation of China, and Peking University Medicine Fund for world's leading discipline or discipline cluster development., Competing Interests: Declaration of interests J.C., S.Y., H.Y., J.W., Z.Z., L.W., S.F., M.S., and W.S. are employees of HUTCHMED Limited., (Copyright © 2025 Elsevier Inc. All rights reserved.)

Powder-based fire extinguishing agents have become a kind of promising substitutes for halon extinguishing agents in civil aircrafts. However, their storage lifespan, significantly influenced by the thermal aging, emerges as a crucial yet overlooked aspect for aviation use. This study investigates the effects of thermal aging cycles on various parameters of ordinary dry powder extinguishing agent (ODPEA) and novel superhydrophobic and oleophobic ultra-fine dry powder extinguishing agent (SHOU DPEA), including surface microscopic morphology, D90 (the diameter at which 90% of the cumulative volume of particles are equal to or smaller than this value), chemical structure, hydrophobic and oleophobic angles, flowability, extinguishing time and effectiveness. The results indicate that SHOU DPEA exhibits smaller particle size, more regular particle shape, significantly superior heat stability and flowability compared to ODPEA. Furthermore, the D90 value evolution of ODPEA with aging time exhibits two stages: (a) a slow and linear growth stage (0-160 days), and (b) a rapid and substantial growth stage (160-200 days). However, SHOU DPEA shows a gradual increase in D90 value throughout the entire accelerated aging process. After 160 days of aging or more, the performance of ODPEA has significantly deteriorated, while SHOU DPEA has shown less degradation. Specially, the extinguishing concentration for the 160-day-aged ODPEA exceeds that of non-aged ODPEA by 10%, whereas the concentration of the 200-day-aged SHOU DPEA is less than 10% of the non-aged counterpart. Additionally, the predicted lifespans for ODPEA and SHOU DPEA at room temperature (25 °C) are 2715 days and over 4525 days, respectively. These findings can provide valuable guidance for assessments and the selection of aviation fire extinguishing agents., Competing Interests: Competing interests: The authors declare no competing interests., (© 2025. The Author(s).)

Several countries around the world are facing the issue of freshwater availability, where agriculture is highly dependent on irrigation, consuming 70% of this vital resource. Water availability is the most limiting factor for the crop production sector and one of the main regulators of the spatial distribution of plants. It is noted that in recent years, the methods of irrigation water application have been improved. Currently, research is directed towards irrigation strategies that reduce water applications. A partial root drying (PRD) technique involves irrigating one-half of the root zone while leaving the other half in relatively dry soil. This method is used in the production of various crops, such as potatoes and cotton. However, the mechanism of PRD, including the physiological and molecular biological processes involved, is not fully understood. In this study, tomato plants were treated with PRD and nitrogen (N) top-dressing. The results showed that PRD could significantly increase the fruit yield, photosynthetic activities, nitrate reductase activity, and fruit quality in the tomato plants, and PRD could also promote the concentrations of oxygen species (O 2 - ), malondialdehyde (MDA) and proline contents, and activities of antioxidant enzymes. In addition, PRD could enhance stress resistance by increasing disease resistance and NP1 and DRED3 antioxidant enzyme activity. Tomato plants treated with PRD compared to the control showed high photosynthetic activity, high yield, better quality of production, and low leaf blight incidence. Overall, the results indicate that PRD is a feasible approach that could be effectively utilized in tomato fields to improve plant growth and production compared with the control.

We analyze the single-photon band structure and the transport of a single photon in a one-dimensional coupled-spinning-resonator chain. The time-reversal symmetry of the resonators chain is broken by the spinning of the resonators, instead of external or synthetic magnetic field. Two nonreciprocal single-photon band gaps can be obtained in the coupled-spinning-resonator chain, whose width depends on the angular velocity of the spinning resonator. Based on the nonreciprocal band gaps, we can implement a single photon circulator at multiple frequency windows, and the direction of photon cycling is opposite for different band gaps. In addition, reciprocal single-photon band structures can also be realized in the coupled-spinning-resonator chain when all resonators rotate in the same direction with equal angular velocity. We believe our work opens a new route to achieve, manipulate, and switch nonreciprocal or reciprocal single-photon band structures, and provides new opportunities to realize novel single-photon devices.

Purpose: Conventional prostate magnetic resonance imaging has limited accuracy for clinically significant prostate cancer (csPCa). We performed diffusion basis spectrum imaging (DBSI) before biopsy and applied artificial intelligence models to these DBSI metrics to predict csPCa., Materials and Methods: Between February 2020 and March 2024, 241 patients underwent prostate MRI that included conventional and DBSI-specific sequences before prostate biopsy. We used artificial intelligence models with DBSI metrics as input classifiers and the biopsy pathology as the ground truth. The DBSI-based model was compared with available biomarkers (PSA, PSA density [PSAD], and Prostate Imaging Reporting and Data System [PI-RADS]) for risk discrimination of csPCa defined as Gleason score > 7., Results: The DBSI-based model was an independent predictor of csPCa (OR 2.04, 95%CI 1.52-2.73, P < .01), as were PSAD (OR 2.02, 95%CI 1.21-3.35, P = .01) and PI-RADS classification (OR 4.00, 95%CI 1.37-11.6 for PI-RADS 3, P = .01; OR 9.67, 95%CI 2.89-32.7, for PI-RADS 4-5, P < .01), adjusting for age, family history, and race. Within our data set, the DBSI-based model alone performed similarly to PSAD + PI-RADS (AUC 0.863 vs 0.859, P = .89), while the combination of the DBSI-based model + PI-RADS had the highest risk discrimination for csPCa (AUC 0.894, P < .01). A clinical strategy using the DBSI-based model for patients with PI-RADS 1-3 could have reduced biopsies by 27% while missing 2% of csPCa (compared with biopsy for all)., Conclusions: Our DBSI-based artificial intelligence model accurately predicted csPCa on biopsy and can be combined with PI-RADS to potentially reduce unnecessary prostate biopsies.

Background: Osteoporosis is a major public health concern characterized by decreased bone density. Among various therapeutic strategies, apoptotic extracellular vesicles (ApoEVs) have emerged as promising agents in tissue regeneration. Specifically, T cell-derived ApoEVs have shown substantial potential in facilitating bone regeneration. However, it remains unclear whether ApoEVs can promote bone mass recovery through enzymatic activity mediated by membrane surface molecules. Therefore, this study aimed to investigate whether T cell-derived ApoEVs could promote bone mass recovery in osteoporosis mice and reveal the underlying mechanisms., Methods: ApoEVs were isolated through sequential centrifugation, and their proteomic profiles were identified via mass spectrometry. Western blot and immunogold staining confirmed the enrichment of CD39 and CD73 on ApoEVs. The role of CD39 and CD73 in hydrolyzing adenosine triphosphate (ATP) to adenosine was evaluated by quantifying the levels of ATP and adenosine. Inhibitors of CD39 and CD73, and an A2BR antagonist were used to explore the molecular mechanism of ApoEVs in promoting bone regeneration., Results: ApoEVs significantly reduced bone loss and promote the osteogenic differentiation of BMMSCs in ovariectomy (OVX) mice. We observed increased levels of extracellular ATP and a decrease in CD39 and CD73, key enzymes in ATP-to-adenosine conversion in bone marrow of OVX mice. We found that ApoEVs are enriched with CD39 and CD73 on their membranes, which enable the hydrolysis of extracellular ATP to adenosine both in vitro and in vivo. The adenosine generated by ApoEVs inhibits the inflammatory response and promotes osteogenesis through A2BR and downstream PKA signaling., Conclusion: T cell-derived ApoEVs are enriched with CD39 and CD73, enabling them to hydrolyze extracellular ATP to adenosine, thereby promoting bone regeneration via A2BR and PKA signaling pathway. Our data underscore the substantive role of T cell-derived ApoEVs to treat osteoporosis, thus providing new ideas for the development of ApoEVs-based therapies in tissue regeneration., Competing Interests: The authors declare no competing interests in this work., (© 2025 Yang et al.)

Accurately and swiftly characterizing the state of polarization (SoP) of complex structured light is crucial in the realms of classical and quantum optics. Conventional strategies for detecting SoP, which typically involves a sequence of cascaded optical elements, are bulky, complex, and run counter to miniaturization and integration. While metasurface-enabled polarimetry has emerged to overcome these limitations, its functionality predominantly remains confined to identifying SoP within the standard Poincaré sphere framework. The comprehensive detection of SoP on the higher-order Poincaré sphere (HOPS), however, continues to be a huge challenge. Here, we propose a general polarization metrology method capable of fully detecting SoP on any HOPS through a single measurement. The underlying mechanism relies on transforming the optical singularities and Stokes parameters into visualized intensity patterns, facilitating the extraction of all parameters that fully determine a SoP. We actualize this concept through a novel meta-device known as the metasurface photonics polarization clock, which offers an intuitive display of SoP using four distinct pointers. As a proof of concept, we theoretically and experimentally demonstrate fully resolving SoPs on the 0th, 1st, and 2nd HOPSs. Our implementation opens up a new pathway towards real-time polarimetry of arbitrary beams featuring miniaturized size, a simple detection process, and a direct readout mechanism, promising significant advancements in fields reliant on polarization., Competing Interests: Conflict of interest: The authors declare no competing interests., (© 2025. The Author(s).)

Nowadays, chemotherapy and immunotherapy remain the major treatment strategies for Triple-Negative Breast Cancer (TNBC). Identifying biomarkers to pre-select and subclassify TNBC patients with distinct chemotherapy responses is essential. In the current study, we performed an unbiased Reverse Phase Protein Array (RPPA) on TNBC cells treated with chemotherapy compounds and found a leading significant increase of phosphor-AURKA/B/C, AURKA, AURKB, and PLK1, which fall into the mitotic kinase group. The increase of AURKA and AURKB protein was majorly due to a post-transcription level regulation, and Paclitaxel treatment induced Aurora Kinases protein phosphorylation on AURKA(T288)/AURKB(T232) sites and their protein stability. In our UAB TNBC cohort, the expression of AURKA and AURKB was significantly higher in TNBC tumors compared to normal adjacent tissues, and AURKB was found to be highly expressed in African American TNBC patients compared to European Americans. Moreover, Aurora Kinases overexpression in TNBC cells renders resistance to Paclitaxel treatment and attenuates the apoptosis effect triggered by chemotherapy treatment, suggesting Aurora Kinases could mediate the chemo-resistance in TNBC. Hence, a combination of Aurora kinase inhibitors or PROTAC degrader and taxane-type chemotherapy significantly enhanced the chemotherapy effect. In summary, we revealed that Aurora Kinases upregulation after treatment with chemotherapy could confer chemotherapy resistance to TNBC cells, and AURKB could serve as preselection markers for stratifying patients' response to neoadjuvant chemotherapy., Competing Interests: Declarations. Ethics approval and consent to participate: This project was approved by the Institutional Review Boards of the University of Alabama at Birmingham (IRB-300011620). Due to the retrospective nature of the study, Institutional Review Board waived the need of obtaining informed consent. Competing interests: The authors declare no competing interests., (© 2025. The Author(s).)

Supernumerary Robotic Limbs (SRLs) are designed to collaborate with the wearer, enhancing operational capabilities. When human limbs are occupied with primary tasks, controlling SRLs flexibly and naturally becomes a challenge. Existing methods such as electromyography (EMG) control and redundant limb control partially address SRL control issues. However, they still face limitations like restricted degrees of freedom and complex data requirements, which hinder their applicability in real-world scenarios. Additionally, fully autonomous control methods, while efficient, often lack the flexibility needed for complex tasks, as they do not allow for real-time user adjustments. In contrast, shared control combines machine autonomy with human input, enabling finer control and more intuitive task completion. Building on our previous work with the mouth-and-tongue interface, this paper integrates a mixed reality (MR) device to form an interactive system that enables shared control of the SRL. The system allows users to dynamically switch between voluntary and autonomous control, providing both flexibility and efficiency. A random forest model classifies 14 distinct tongue and mouth operations, mapping them to six-degree-of-freedom SRL control. In comparative experiments involving ten healthy subjects performing assembly tasks under three control modes (shared control, autonomous control, and voluntary control), shared control demonstrates a balance between machine autonomy and human input. While autonomous control offers higher task efficiency, shared control achieves greater task success rates and improves user experience by combining the advantages of both autonomous operation and voluntary control. This study validates the feasibility of shared control and highlights its advantages in providing flexible switching between autonomy and user intervention, offering new insights into SRL control.

Background: Three years into the SARS-CoV-2 pandemic, the virus continues to mutate despite widespread vaccination, posing ongoing challenges for epidemic prevention and control. The relationship between viral shedding and immune escape remains under investigation. This study aims to examine the association between viral shedding and immune escape in the BA.4/5 and BF.7 variants., Method: We included 542 patients infected with the Omicron variant from Beijing Xiaotangshan shelter hospital. Based on the viral strain, patients were divided into BA.4/5 group and BF.7 group. Additionally, we categorized patients into rapid viral shedding and slow viral shedding groups according to their viral shedding rates. We explored the relationship between viral shedding and immune-related clinical indicators during this period., Result: Of the 542 patients, 118 were infected with BA.4/5 variant, and 424 were infected with BF.7 variant. The viral shedding duration differed significantly between BA.4/5 and BF.7 groups ( p  < 0.0001). However, there was no statistically significant correlation between viral shedding duration and immune-related indicators, such as WBC, Hb, PLT, Neu, Lym, CRP, allergy, fever, and vaccination status ( p  > 0.05). Furthermore, viral shedding duration was not associated with vaccination status, intervals between vaccinations, or vaccine types ( p  > 0.05)., Conclusion: The duration of viral shedding in patients infected with Omicron variants BA.4/5 and BF.7 is not associated with WBC, Hb, Lym, CRP, fever, allergy, or vaccine-related indicators. This lack of association may be attributed to immune escape mechanisms., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2025 Zhang, Gai, Duan, Yan, Huang, Wu, Zheng, Lin, Zhou, Dai, Yang, Bao, Jing, Cai, Ma and Sun.)

Objective: To compare the clinical outcomes between radiofrequency ablation (RFA) and microwave ablation (MWA) for the treatment of T1N0M0 papillary thyroid carcinoma (PTC) in a large cohort., Materials and Methods: This retrospective study included 1111 patients with solitary T1N0M0 PTC treated with RFA (n = 894) or MWA (n = 215) by experienced physicians. A propensity score matching was used to compare disease progression, including lymph node metastases (LNM), recurrent tumors and persistent tumors, recurrence-free survival (RFS), volume reduction ratio (VRR), and complications between the RFA and MWA groups. The subgroup outcomes of T1a and T1b tumors treated with RFA and MWA were also compared., Results: During a mean follow-up period of 41.7 ± 12.6 months, the overall incidence rates of disease progression, LNM, recurrent tumors, and persistent tumors were 2.1%, 0.7%, 1.2%, and 0.2%, respectively. Eight patients (0.7%) experienced transient voice change and recovered within 3 months. After 1:1 matching, there were no significant differences in disease progression (3.3% vs. 3.7%, p = 0.793), LNM (1.4% vs. 1.9%, p = 1.000), recurrent tumor (1.4% vs. 1.9%, p = 1.000), persistent tumors (0.5% vs. 0%, p = 0.317), RFS rates (96.7% vs. 96.3%, p = 0.821), VRR (99.3 ± 3.0% vs. 98.3 ± 8.5%, p = 0.865), tumor disappearance rate (87.9% vs. 81.4%, p = 0.061) and complications (1.4% vs. 1.9%, p = 1.000) between the RFA and MWA groups. For T1a and T1b tumors, clinical outcomes were comparable between RFA and MWA., Conclusions: RFA and MWA did not show significant differences in clinical outcomes for T1N0M0 PTC. The choice between RFA and MWA could depend on physician expertise and resources of local center resources. A qualified and experienced physician with extensive knowledge of the modalities is recommended when thermal ablation is performed., Key Points: Question With radiofrequency ablation (RFA) and microwave ablation (MWA) used worldwide for T1N0M0 papillary thyroid carcinoma (PTC), is one of them superior to the others? Findings There were no significant differences in disease progression, recurrence-free survival, volume reduction ratio, tumor disappearance, or complications between techniques for solitary T1N0M0 papillary thyroid carcinoma. Clinical relevance The choice between RFA and MWA could depend on physician expertise and resources of the local center resources. A qualified and experienced physician with extensive knowledge of the modalities is recommended when thermal ablation is performed., Competing Interests: Compliance with ethical standards. Guarantor: The scientific guarantor of this publication is Yukun Luo. Conflict of interest: The authors of this manuscript declare no relationships with any companies, whose products or services may be related to the subject matter of the article. Statistics and biometry: No complex statistical methods were necessary for this paper. Informed consent: The requirement for obtaining informed consent for patients was waived because of its retrospective nature. All patients had provided written informed consent prior to treatment. Ethical approval: Institutional Review Board approval was obtained. Study subjects or cohorts overlap: Study subjects or cohorts have never been previously. Methodology: Retrospective Diagnostic or prognostic study Performed at one institution, (© 2025. The Author(s), under exclusive licence to European Society of Radiology.)