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Your search keyword '"Gladyshev, Vadim N."' showing total 2,137 results
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2,137 results on '"Gladyshev, Vadim N."'

202. A chromosome-level genome of Antechinus flavipes provides a reference for an Australian marsupial genus with male death after mating

Author

Tian, Ran, Han, Kai, Geng, Yuepan, Yang, Chen, Shi, Chengcheng, Thomas, Patrick B., Pearce, Coral, Moffatt, Kate, Ma, Siming, Xu, Shixia, Yang, Guang, Zhou, Xuming, Gladyshev, Vadim N., Liu, Xin, Fisher, Diana O., Chopin, Lisa K., Leiner, Natália O., Baker, Andrew M., Fan, Guangyi, Seim, Inge, Tian, Ran, Han, Kai, Geng, Yuepan, Yang, Chen, Shi, Chengcheng, Thomas, Patrick B., Pearce, Coral, Moffatt, Kate, Ma, Siming, Xu, Shixia, Yang, Guang, Zhou, Xuming, Gladyshev, Vadim N., Liu, Xin, Fisher, Diana O., Chopin, Lisa K., Leiner, Natália O., Baker, Andrew M., Fan, Guangyi, and Seim, Inge

Abstract

The 15 species of small carnivorous marsupials that comprise the genus Antechinus exhibit semelparity, a rare life-history strategy in mammals where synchronized death occurs after one breeding season. Antechinus males, but not females, age rapidly (demonstrate organismal senescence) during the breeding season and show promise as new animal models of ageing. Some antechinus species are also threatened or endangered. Here, we report a chromosome-level genome of a male yellow-footed antechinus Antechinus flavipes. The genome assembly has a total length of 3.2 Gb with a contig N50 of 51.8 Mb and a scaffold N50 of 636.7 Mb. We anchored and oriented 99.7% of the assembly on seven pseudochromosomes and found that repetitive DNA sequences occupy 51.8% of the genome. Draft genome assemblies of three related species in the subfamily Phascogalinae, two additional antechinus species (Antechinus argentus and A. arktos) and the iteroparous sister species Murexia melanurus, were also generated. Preliminary demographic analysis supports the hypothesis that climate change during the Pleistocene isolated species in Phascogalinae and shaped their population size. A transcriptomic profile across the A. flavipes breeding season allowed us to identify genes associated with aspects of the male die-off. The chromosome-level A. flavipes genome provides a steppingstone to understanding an enigmatic life-history strategy and a resource to assist the conservation of antechinuses.

Published
2022

203. Translation elongation factor 2 depletion by siRNA in mouse liver leads to mTOR-independent translational upregulation of ribosomal protein genes

Author

Gerashchenko, Maxim V., Nesterchuk, Mikhail V., Smekalova, Elena M., Paulo, Joao A., Kowalski, Piotr S., Akulich, Kseniya A., Bogorad, Roman, Dmitriev, Sergey E., Gygi, Steven, Zatsepin, Timofei, Anderson, Daniel G., Gladyshev, Vadim N., Koteliansky, Victor E., Gerashchenko, Maxim V., Nesterchuk, Mikhail V., Smekalova, Elena M., Paulo, Joao A., Kowalski, Piotr S., Akulich, Kseniya A., Bogorad, Roman, Dmitriev, Sergey E., Gygi, Steven, Zatsepin, Timofei, Anderson, Daniel G., Gladyshev, Vadim N., and Koteliansky, Victor E.

Abstract

© 2020, The Author(s). Due to breakthroughs in RNAi and genome editing methods in the past decade, it is now easier than ever to study fine details of protein synthesis in animal models. However, most of our understanding of translation comes from unicellular organisms and cultured mammalian cells. In this study, we demonstrate the feasibility of perturbing protein synthesis in a mouse liver by targeting translation elongation factor 2 (eEF2) with RNAi. We were able to achieve over 90% knockdown efficacy and maintain it for 2 weeks effectively slowing down the rate of translation elongation. As the total protein yield declined, both proteomics and ribosome profiling assays showed robust translational upregulation of ribosomal proteins relative to other proteins. Although all these genes bear the TOP regulatory motif, the branch of the mTOR pathway responsible for translation regulation was not activated. Paradoxically, coordinated translational upregulation of ribosomal proteins only occurred in the liver but not in murine cell culture. Thus, the upregulation of ribosomal transcripts likely occurred via passive mTOR-independent mechanisms. Impaired elongation sequesters ribosomes on mRNA and creates a shortage of free ribosomes. This leads to preferential translation of transcripts with high initiation rates such as ribosomal proteins. Furthermore, severe eEF2 shortage reduces the negative impact of positively charged amino acids frequent in ribosomal proteins on ribosome progression.

Published
2022

204. Meeting Report:Aging Research and Drug Discovery

Author

Meron, Esther, Thaysen, Maria, Angeli, Suzanne, Antebi, Adam, Barzilai, Nir, Baur, Joseph A., Bekker-Jensen, Simon, Birkisdottir, Maria, Bischof, Evelyne, Bruening, Jens, Brunet, Anne, Buchwalter, Abigail, Cabreiro, Filipe, Cai, Shiqing, Chen, Brian H., Ermolaeva, Maria, Ewald, Collin Y., Ferrucci, Luigi, Florian, Maria Carolina, Fortney, Kristen, Freund, Adam, Georgievskaya, Anastasia, Gladyshev, Vadim N., Glass, David, Golato, Tyler, Gorbunova, Vera, Hoejimakers, Jan, Houtkooper, Riekelt H., Jager, Sibylle, Jaksch, Frank, Janssens, Georges, Jensen, Martin Borch, Kaeberlein, Matt, Karsenty, Gerard, de Keizer, Peter, Kennedy, Brian, Kirkland, James L., Kjaer, Michael, Kroemer, Guido, Lee, Kai Fu, Lemaitre, Jean Marc, Liaskos, David, Longo, Valter D., Lu, Yu Xuan, MacArthur, Michael R., Maier, Andrea B., Manakanatas, Christina, Mitchell, Sarah J., Moskalev, Alexey, Niedernhofer, Laura, Ozerov, Ivan, Partridge, Linda, Passegué, Emmanuelle, Petr, Michael A., Peyer, James, Radenkovic, Dina, Rando, Thomas A., Rattan, Suresh, Riedel, Christian G., Rudolph, Lenhard, Ai, Ruixue, Serrano, Manuel, Schumacher, Björn, Sinclair, David A., Smith, Ryan, Suh, Yousin, Taub, Pam, Trapp, Alexandre, Trendelenburg, Anne Ulrike, Valenzano, Dario Riccardo, Verburgh, Kris, Verdin, Eric, Vijg, Jan, Westendorp, Rudi G.J., Zonari, Alessandra, Bakula, Daniela, Zhavoronkov, Alex, Scheibye-Knudsen, Morten, Meron, Esther, Thaysen, Maria, Angeli, Suzanne, Antebi, Adam, Barzilai, Nir, Baur, Joseph A., Bekker-Jensen, Simon, Birkisdottir, Maria, Bischof, Evelyne, Bruening, Jens, Brunet, Anne, Buchwalter, Abigail, Cabreiro, Filipe, Cai, Shiqing, Chen, Brian H., Ermolaeva, Maria, Ewald, Collin Y., Ferrucci, Luigi, Florian, Maria Carolina, Fortney, Kristen, Freund, Adam, Georgievskaya, Anastasia, Gladyshev, Vadim N., Glass, David, Golato, Tyler, Gorbunova, Vera, Hoejimakers, Jan, Houtkooper, Riekelt H., Jager, Sibylle, Jaksch, Frank, Janssens, Georges, Jensen, Martin Borch, Kaeberlein, Matt, Karsenty, Gerard, de Keizer, Peter, Kennedy, Brian, Kirkland, James L., Kjaer, Michael, Kroemer, Guido, Lee, Kai Fu, Lemaitre, Jean Marc, Liaskos, David, Longo, Valter D., Lu, Yu Xuan, MacArthur, Michael R., Maier, Andrea B., Manakanatas, Christina, Mitchell, Sarah J., Moskalev, Alexey, Niedernhofer, Laura, Ozerov, Ivan, Partridge, Linda, Passegué, Emmanuelle, Petr, Michael A., Peyer, James, Radenkovic, Dina, Rando, Thomas A., Rattan, Suresh, Riedel, Christian G., Rudolph, Lenhard, Ai, Ruixue, Serrano, Manuel, Schumacher, Björn, Sinclair, David A., Smith, Ryan, Suh, Yousin, Taub, Pam, Trapp, Alexandre, Trendelenburg, Anne Ulrike, Valenzano, Dario Riccardo, Verburgh, Kris, Verdin, Eric, Vijg, Jan, Westendorp, Rudi G.J., Zonari, Alessandra, Bakula, Daniela, Zhavoronkov, Alex, and Scheibye-Knudsen, Morten

Abstract

Aging is the single largest risk factor for most chronic diseases, and thus possesses large socioeconomic interest to continuously aging societies. Consequently, the field of aging research is expanding alongside a growing focus from the industry and investors in aging research. This year's 8th Annual Aging Research and Drug Discovery (ARDD) meeting was organized as a hybrid meeting from August 30th to September 3rd 2021 with more than 130 attendees participating on-site at the Ceremonial Hall at University of Copenhagen, Denmark, and 1800 engaging online. The conference comprised of presentations from 75 speakers focusing on new research in topics including mechanisms of aging and how these can be modulated as well as the use of AI and new standards of practices within aging research. This year, a longevity workshop was included to build stronger connections with the clinical community.

Published
2022

210. Roles of the 15-kDa Selenoprotein (Sep15) in Redox Homeostasis and Cataract Development Revealed by the Analysis of Sep 15 Knockout Mice

Author

Kasaikina, Marina V., Fomenko, Dmitri E., Labunskyy, Vyacheslav M., Lachke, Salil A., Qiu, Wenya, Moncaster, Juliet A., Zhang, Jie, Wojnarowicz, Mark W., Jr., Natarajan, Sathish Kumar, Malinouski, Mikalai, Schweizer, Ulrich, Tsuji, Petra A., Carlson, Bradley A., Maas, Richard L., Lou, Marjorie F., Goldstein, Lee E., Hatfield, Dolph L., and Gladyshev, Vadim N.

Published
2011
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223. Biological age is increased by stress and restored upon recovery

Author

Poganik, Jesse R., primary, Zhang, Bohan, additional, Baht, Gurpreet S., additional, Kerepesi, Csaba, additional, Yim, Sun Hee, additional, Lu, Ake T., additional, Haghani, Amin, additional, Gong, Tong, additional, Hedman, Anna M., additional, Andolf, Ellika, additional, Pershagen, Göran, additional, Almqvist, Catarina, additional, White, James P., additional, Horvath, Steve, additional, and Gladyshev, Vadim N., additional

Published
2022
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232. Rapamycin treatment during development extends lifespan and healthspan

Author

Shindyapina, Anastasia V., primary, Cho, Yongmin, additional, Kaya, Alaattin, additional, Tyshkovskiy, Alexander, additional, Castro, José P., additional, Gordevicius, Juozas, additional, Poganik, Jesse R., additional, Horvath, Steve, additional, Peshkin, Leonid, additional, and Gladyshev, Vadim N., additional

Published
2022
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233. Naked Mole-Rat Hyaluronan Synthase 2 Promotes Longevity and Enhances Healthspan in Mice

Author

Zhang, Zhihui, primary, Tian, Xiao, additional, Lu, J. Yuyang, additional, Boit, Kathryn, additional, Ablaeva, Julia, additional, Tolibzoda Zakusilo, Frances, additional, Emmrich, Stephan, additional, Firsanov, Denis, additional, Rydkina, Elena, additional, Biashad, Seyed Ali, additional, Lu, Quan, additional, Tyshkovsky, Alexander, additional, Gladyshev, Vadim N., additional, Horvath, Steve, additional, Seluanov, Andrei, additional, and Gorbunova, Vera, additional

Published
2022
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236. Biosensor-Linked Immunosorbent Assay for the Quantification of Methionine Oxidation in Target Proteins

Author

Lee, Hae Min, primary, Choi, Dong Wook, additional, Kim, Seahyun, additional, Lee, Aro, additional, Kim, Minseo, additional, Roh, Yeon Jin, additional, Jo, Young Ho, additional, Cho, Hwa Yeon, additional, Lee, Ho-Jae, additional, Lee, Seung-Rock, additional, Tarrago, Lionel, additional, Gladyshev, Vadim N., additional, Kim, Ji Hyung, additional, and Lee, Byung Cheon, additional

Published
2021
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