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204. Experimental and clinical employment of end-to-side coaptation: our experience

Author

Tos, Pierluigi, Geuna, Stefano, Papalia, I, Conforti, Lg, Artiaco, S, and Battiston, B.

Subjects
Adult, Male, Suture Techniques, Peripheral Nervous System Diseases, Recovery of Function, Middle Aged, Neurosurgical Procedures, Nerve Regeneration, Rats, Disease Models, Animal, Young Adult, Animals, Humans, Female, Rats, Wistar, Follow-Up Studies, Retrospective Studies
Abstract

The last 15 years have seen a growing interest regarding a technique for nerve repair named end-to-side coaptation. Since 2000, we have carried out experimental studies on end-to-side nerve repair as well as employed this technique to a series of selected clinical cases. Here we report on the results of this experience.For experimental studies, we have used the model represented by median nerve repair by end-to-side coaptation either on the ulnar (agonistic) or the radial (antagonistic) nerve. For time course assessment of median nerve functional recovery we used the grasping test, a test which permits to assess voluntary control of muscle function. Repaired nerves were processed for resin embedding to allow nerve fibre stereology and electron microscopy. Results showed that, in either experimental group, end-to-side-repaired median nerves were repopulated by axons regenerating from ulnar and radial donor nerves, respectively. Moreover, contrary to previously published data, our results showed that voluntary motor control of the muscles innervated by the median nerve was progressively recovered also when the antagonistic radial nerve was the donor nerve.As regards our clinical experience, results were not so positive. We have treated by end-to-side coaptation patients with both sensory (n = 7, collateral digital nerves) and mixed (n = 8, plexus level) nerve lesions. Results were good, as in other series, in sensory nerves whilst they were very difficult to investigate in mixed nerves at the plexus level.Take together, these results suggest that clinical employment of end-to-side coaptation should still be considered at the moment as the ultima ratio in cases in which no other repair technique can be attempted. Yet, it is clear that more basic research is needed to explain the reasons for the different results between laboratory animal and humans and, especially, to find out how to ameliorate the outcome of end-to-side nerve repair by adequate treatment and rehabilitation.

Published
2010

210. Comparison of results between chitosan hollow tube and autologous nerve graft in reconstruction of peripheral nerve defect: An experimental study

Author

Shapira, Yuval, primary, Tolmasov, Michael, additional, Nissan, Moshe, additional, Reider, Evgeniy, additional, Koren, Akiva, additional, Biron, Tali, additional, Bitan, Yifat, additional, Livnat, Mira, additional, Ronchi, Giulia, additional, Geuna, Stefano, additional, and Rochkind, Shimon, additional

Published
2015
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211. Corrigendum to “Postconditioning induces an anti-apoptotic effect and preserves mitochondrial integrity in isolated rat hearts” [Biochim. Biophys. Acta. 1787 (2009) 794–801]

Author

Penna, Claudia, primary, Perrelli, Maria-Giulia, additional, Raimondo, Stefania, additional, Tullio, Francesca, additional, Merlino, Annalisa, additional, Moro, Francesca, additional, Geuna, Stefano, additional, Mancardi, Daniele, additional, and Pagliaro, Pasquale, additional

Published
2015
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214. AAV vector encoding human VEGF165–transduced pectineus muscular flaps increase the formation of new tissue through induction of angiogenesis in an in vivo chamber for tissue engineering: A technique to enhance tissue and vessels in microsurgically engineered tissue

Author

Moimas, Silvia, primary, Manasseri, Benedetto, additional, Cuccia, Giuseppe, additional, Stagno d’Alcontres, Francesco, additional, Geuna, Stefano, additional, Pattarini, Lucia, additional, Zentilin, Lorena, additional, Giacca, Mauro, additional, and Colonna, Michele R, additional

Published
2015
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222. Tissue engineering of peripheral nerves

Author

Raimondo, Stefania, Tos, Pierluigi, Fornaro, Michele, Gambarotta, Giovanna, Perroteau, Isabelle, Robecchi, Mariagiuseppina, Battiston, B, and Geuna, Stefano

Published
2010

227. Nanotechnology versus stem cell engineering: in vitro comparison of neurite inductive potentials

Author

Morano,Michela, Wrobel,Sandra, Fregnan,Federica, Ziv-Polat,Ofra, Shahar,Abraham, Ratzka,Andreas, Grothe,Claudia, Geuna,Stefano, Haastert-Talini,Kirsten, Morano,Michela, Wrobel,Sandra, Fregnan,Federica, Ziv-Polat,Ofra, Shahar,Abraham, Ratzka,Andreas, Grothe,Claudia, Geuna,Stefano, and Haastert-Talini,Kirsten

Abstract

Michela Morano,1,* Sandra Wrobel,2,3,* Federica Fregnan,1 Ofra Ziv-Polat,4 Abraham Shahar,4 Andreas Ratzka,2 Claudia Grothe,2,3 Stefano Geuna,1 Kirsten Haastert-Talini2,3 1Department of Clinical and Biological Sciences, Università Degli Studi di Torino, Orbassano, Piemonte, Italy; 2Institute of Neuroanatomy, Hannover Medical School, Hannover, Lower-Saxony, Germany; 3Center for Systems Neuroscience (ZSN), Hannover, Lower-Saxony, Germany; 4NVR Research Ltd, Ness-Ziona, Israel *These authors contributed equally to this work and share first authorship Purpose: Innovative nerve conduits for peripheral nerve reconstruction are needed in order to specifically support peripheral nerve regeneration (PNR) whenever nerve autotransplantation is not an option. Specific support of PNR could be achieved by neurotrophic factor delivery within the nerve conduits via nanotechnology or stem cell engineering and transplantation.Methods: Here, we comparatively investigated the bioactivity of selected neurotrophic factors conjugated to iron oxide nanoparticles (np-NTFs) and of bone marrow-derived stem cells genetically engineered to overexpress those neurotrophic factors (NTF-BMSCs). The neurite outgrowth inductive activity was monitored in culture systems of adult and neonatal rat sensory dorsal root ganglion neurons as well as in the cell line from rat pheochromocytoma (PC-12) cell sympathetic culture model system.Results: We demonstrate that np-NTFs reliably support numeric neurite outgrowth in all utilized culture models. In some aspects, especially with regard to their long-term bioactivity, np-NTFs are even superior to free NTFs. Engineered NTF-BMSCs proved to be less effective in induction of sensory neurite outgrowth but demonstrated an increased bioactivity in the PC-12 cell culture system. In contrast, primary nontransfected BMSCs were as effective as np-NTFs in sensory neurite induction and demonstrated an impairment of neuronal differentiation in the PC-12

Published
2014

228. Gene transfer for promoting nerve regeneration

Author

Geuna, Stefano, Raimondo, Stefania, Ronchi, Giulia, Mazzucco, A, Audisio, Chiara, Fregnan, Federica, Gambarotta, Giovanna, Perroteau, Isabelle, Novati, F, Arnez, Z, Moimas, S, Zacchigna, S, Zentilin, L, and Giacca, M.

Subjects
peripheral nerve regeneration, gene therapy
Published
2009

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