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674 results on '"Gamba, Piergiorgio"'

202. Analgesia fetale in corso di manovre invasive

Author

D'Antona, Donato, Pitton, Ma, Micaglio, M, Gamba, Piergiorgio, Ambrosini, Guido, Cosmi, Erich, Trevisanuto, D, Zanardo, V, Monegato, Giovanna, DI LENARDO, L, and Nardelli, GIOVANNI BATTISTA

Published
2004

203. Ernia ombelicale

Author

Battocchio, F., Antoniello, L. M., Tristano, W., and Gamba, Piergiorgio

Published
2004

215. First results of a European multi-center registry of patients with anorectal malformations

Author

de Blaauw, Ivo, primary, Wijers, Charlotte H.W., additional, Schmiedeke, Eberhard, additional, Holland-Cunz, Stefan, additional, Gamba, Piergiorgio, additional, Marcelis, Carlo L.M., additional, Reutter, Heiko, additional, Aminoff, Dalia, additional, Schipper, Muriel, additional, Schwarzer, Nicole, additional, Grasshoff-Derr, Sabine, additional, Midrio, Paola, additional, Jenetzky, Ekkehart, additional, and van Rooij, Iris A.L.M., additional

Published
2013
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217. New human embryo liver cell lines obtained by stabilization and immortalization enhance in vitro clonal growth of cordonal blood cells

Author

DEL PUP, Laura, DE ANGELI, Sergio, Conconi, MARIA TERESA, Grandi, Claudio, Gamba, Piergiorgio, Parnigotto, PIER PAOLO, and Nusdorfer, Gastone

Subjects
Reverse Transcriptase Polymerase Chain Reaction, liver cell line, Infant, Newborn, Gene Expression, immortalization, Embryo, Mammalian, Fetal Blood, human embryo, Immunohistochemistry, Coculture Techniques, Cell Line, Colony-Forming Units Assay, Liver, Karyotyping, Cytokines, Humans, human embryo, liver cell line, immortalization, cordonal blood cell, cordonal blood cell, Biomarkers, Cell Division
Abstract

We developed two new cell lines derived from embryo liver and tested their inductive capacity on in vitro clonal growth of cordonal blood (CB) hematopoietic cells. One line was stabilized and named BAEP2-WILD (W), and the other one was immortalized by retroviral transduction with SV40 Large T antigen and called BAEP2-SV40. Southern blot analysis demonstrated the integration of the Large T antigen gene in the BAEP2-SV40 cell genome, but this line did not display the expected growth arrest at the non-permissive temperature of 39 degrees C. Immunocytochemistry showed that BAEP2-SV40 cell line was positive for several cytokeratins and stromal markers (vimentin, desmin and laminin), as well as for epidermal growth factor (EGF), fibroblast growth factor (FGF) and their receptors (Rs). In contrast, BAEP2-W evidenced positivity only for cytokeratin-7 and laminin, and low positivity to EGF, EGF-R, FGF and FGF-R. BAEP2-SV40 cell line, but not BAEP2-W, expressed interleukin (IL)-1, IL-6, granulocyte macrophage-colony stimulating factor (GM-CSF), granulocyte-colony stimulating factor, stem cell factor and vascular-cell adhesion molecule-1 mRNAs, and secreted IL-6 and GM-CSF. Taken together, these findings could suggest that BAEP2-W cell line possesses the phenotype of fetal hepatocytes, while BAEP2-SV40 cell line has that of stromal cells. The supernatants conditioned by both cell lines stimulated the clonal growth of CB hematopoietic cells cultured on semisolid media deprived of growth factors and cytokines, the inductive capacity of the BAEP2-SV40 cell line being markedly higher than that of its wild counterpart, conceivably due to its ability to produce cytokines. Our study indicates that these two new cell lines, and especially BAEP2-SV40 one could be used in co-culture systems as feeder-layers for hematopoietic CB SC expansion in vitro.

Published
2002

235. Work-related upper limb musculoskeletal disorders in paediatric laparoscopic surgery. A multicenter survey

Author

Esposito, Ciro, primary, Ghoneimi, Alaa El, additional, Yamataka, Atsuyuki, additional, Rothenberg, Steve, additional, Bailez, Marcela, additional, Ferro, Marcelo, additional, Gamba, Piergiorgio, additional, Castagnetti, Marco, additional, Mattioli, Girolamo, additional, Delagausie, Pascale, additional, Antoniou, Dimitris, additional, Montupet, Philippe, additional, Marte, Antonio, additional, Saxena, Amulya, additional, Bertozzi, Mirko, additional, Philippe, Paul, additional, Varlet, François, additional, Lardy, Hubert, additional, Caldamone, Antony, additional, Settimi, Alessandro, additional, Pelizzo, Gloria, additional, Becmeur, Francois, additional, Escolino, Maria, additional, Pascale, Teresa De, additional, Najmaldin, Azad, additional, and Schier, Felix, additional

Published
2013
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240. Complications of percutaneous endoscopic gastrostomy in children: Results of an Italian multicenter observational study

Author

Fascetti-Leon, Francesco, primary, Gamba, PierGiorgio, additional, Dall’Oglio, Luigi, additional, Pane, Alessandro, additional, de’ Angelis, Gian Luigi, additional, Bizzarri, Barbara, additional, Fava, Giorgio, additional, Maestri, Luciano, additional, Cheli, Maurizio, additional, Di Nardo, Giovanni, additional, La Riccia, Antonio, additional, Marrello, Saverio, additional, Gandullia, Paolo, additional, Romano, Claudio, additional, D’Antiga, Lorenzo, additional, and Betalli, Pietro, additional

Published
2012
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242. ECMO ed ernia diaframmatica

Author

Gamba, Piergiorgio, Midrio, P., Gasparella, Marco, Biban, P., Pettenazzo, A., and Previtera, C.

Published
1995

244. Minimally invasive resection of adrenal masses in infants and children: results of a European multi-center survey

Author

Fascetti-Leon, Francesco, Scotton, Giovanni, Pio, Luca, Beltrà, Raimundo, Caione, Paolo, Esposito, Ciro, Mattioli, Girolamo, Saxena, Amulya, Sarnacki, Sabine, and Gamba, Piergiorgio

Abstract

Minimal access adrenal surgery (MAAS) for adrenal pathologies is the standard for many pediatric surgical centers. However, the literature offers few reports and minimal evidence from small case series. The aim of this study was to evaluate the outcomes of pediatric MAAS through a multi-center data analysis. Pediatric patients who underwent MAAS between January 2002 and December 2013 were retrospectively included. Data analysis was conducted using Spss software (Welch's t-test, X-square, Fisher tests, multiple regression model). Six European centers participated, 68 patients were included with mean age of 5.2 years (2 months–16 years). Lesion volume was of 18.1 cc (0.78–145.6), with a mean diameter of 2.8 cm (1.1–6.5). Localization was 50% left-sided masses, 45.6% right-side masses, and 4.4% bilateral. Histological examination revealed 36 neuroblastomas, 15 adenomas, nine pheochromocytomas, three ganglioneuromas, two ganglioneuroblastomas, one bilateral hyperplasia, one adrenocortical carcinoma, an alveolar sarcoma, and a calcification. Surgical access was transperitoneal in 63 (92.6%) and retroperitoneal in 5 (7.4%). Mean operative time was 170 ± 87 min (285 ± 30 min for bilateral lesions). Mean hospital stay was 4.2 ± 2.5 days. Complications included blood loss requiring transfusion in five patients (7.4%) and a diaphragmatic tear. Infiltration of surrounding structures correlated with intraoperative complication rate (p= 0.027) and operative time (p< 0.01). No mass rupture, conversion, or post-operative complications were observed. Median follow-up was 52 months (1–161). Two recurrences occurred in patients with pheochromocytoma. Age, weight, symptoms, characteristics at imaging, chemistry, volume, or histology, did not influence operative time, hospital stays, or complication rate. Pediatric MAAS was safe adopted for masses up to 145.6 cc, with a very low rate of complication. Conversion to open is not necessary even in the presence of infiltrations. MAAS should represent the first-line treatment for selected cases in centers experienced in laparoscopy.

Published
2017
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