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1,451 results on '"Fujii, R."'

212. Adenosine triphosphate-dependent transport of leukotriene C4 by membrane vesicles prepared from cisplatin-resistant human epidermoid carcinoma tumor cells.

Author

Fujii R, Mutoh M, Sumizawa T, Chen ZS, Yoshimura A, Akiyama S, Fujii, R, Mutoh, M, Sumizawa, T, Chen, Z S, Yoshimura, A, and Akiyama, S

Abstract

Background: Cisplatin accumulation is decreased in many cisplatin-resistant cells. An active efflux pump for cisplatin exists in cisplatin-resistant human epidermoid carcinoma cells (called KB cells). A previous study has suggested that the adenosine triphosphate (ATP)-dependent glutathione S-conjugate export pump (GS-X pump), which exports the bis-(glutathionato)-platinum (II) (GS-platinum) complex, could contribute to cellular resistance to cisplatin.Purpose: In this study, we examined whether the active efflux pump for cisplatin in the cisplatin-resistant KB cells is the GS-X pump and tested its activity by using an endogenous substrate, [3H]leukotriene C4 ([3H]LTC4).Methods: Membrane vesicles were prepared from KB-3-1 (clone from parental KB cells) cells and from cisplatin-resistant KCP-4 (a mutant clone derived from KB-3-1 cells) cells. Using a filtration technique, we measured the uptake and transport of [3H]LTC4, a substrate for the GS-X pump, into membrane vesicles at 37 degrees C.Results: The uptake of [3H]LTC4 in the membrane vesicles from both the KB-3-1 and KCP-4 cells was ATP-dependent. In contrast, the ATP-dependent transport of [3H]LTC4 was observed only in KCP-4 membrane vesicles but not in KB-3-1 membrane vesicles. The ATP-dependent transport was vanadate sensitive and was inhibited by GS-platinum complex but only marginally by cisplatin and glutathione and not by vincristine or verapamil. The nucleotide triphosphates, guanosine triphosphate, cytidine triphosphate, uridine triphosphate, and deoxythymidine triphosphate could be substituted for ATP but were less efficient. A nonhydrolyzable ATP analogue, adenosine 5'-(beta,gamma-methylene) triphosphate, was not effective.Conclusions: The transport of LTC4 in membrane vesicles prepared from KCP-4 cells was facilitated by an ATP-dependent pump that appeared very similar to the GS-X pump.Implications: Our study suggests that the GS-X pump is involved in the decreased accumulation of cisplatin in KCP-4 cells. [ABSTRACT FROM AUTHOR]

Published
1994
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214. Management and optimization of environmental information using integrated technology: a case study in the city of São Paulo, Brazil.

Author

Kauffman, Joanne M., Morrison, Gregory M., Rauch, Sébastien, Nedel, A S, Oyola, P, Fujii, R K, and Cacavallo, R C

Abstract

In the São Paulo metropolitan region, like other world's big urban centres, environmental monitoring plays a very important role, in view of the degradation of air quality. This work illustrates a case study of continuous monitoring of air pollutants using a differential optical absorption spectrometer (DOAS) associated with an Integrated Environmental Management System (AIRVIRO) in São Paulo, Brazil. The DOAS registered concentrations of benzene, toluene, m-p xilenes, O3, SO2, NO2, NO3, and HNO, and also of a meteorological station continuous data collection. Air quality databases from CETESB - State Environmental Authority were used for reference. Results of days with high concentration averages in winter are related to specific meteorological conditions and local traffic. [ABSTRACT FROM AUTHOR]

Published
2007
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215. Air pollution levels in two São Paulo subway stations.

Author

Kauffman, Joanne M., Morrison, Gregory M., Rauch, Sébastien, Fujii, R K, Oyola, P, Pereira, J C R, Nedel, A S, and Cacavallo, R C

Abstract

In megacities like São Paulo, which is responsible for approximately 16% of Brazil's gross national product (GNP), the subway serves as a major transportation mode, transporting 2.7 million people daily. The underground portion of the subway system is a confined space that may permit the concentration of contaminants either from the outside atmosphere or generated internally. The objective of this study is to evaluate the air quality of two of São Paulo's subway stations (Clínicas and Praça da Sé), by identifying the fungi species, the nitrogen dioxide (NO2), BTEX (benzene, toluene, ethylbenzene, and xylene), and particulate material (PM10) concentrations. Afterwards, a comparison of the internal pollutants concentration values with external atmosphere parameters is performed. [ABSTRACT FROM AUTHOR]

Published
2007
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226. FUSION OF LIGHT WEAKLY BOUND NUCLEI

Author

Szanto de Toledo, A., primary, Alonso, E., additional, Carlin, N., additional, Fujii, R. J., additional, de Moura, M. M., additional, Munhoz, M. G., additional, Souza, F. A., additional, Suaide, A. A. P, additional, Szanto, E. M., additional, and Takahashi, J., additional

Published
2001
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229. FUSION OF LIGHT WEAKLY BOUND NUCLEI.

Author

de Toledo, A. Szanto, Alonso, E., Carlin, N., Fujii, R. J., de Moura, M. M., Munhoz, M. G., Souza, F. A., Suaide, A. A. P., Szanto, E. M., and Takahashi, J.

Subjects
NUCLEAR fusion, NUCLEAR reactions, COULOMB barriers (Nuclear fusion), BINDING energy, NUCLEON-nucleon interactions
Published
2001

231. Mutations in the Hepatocyte Nuclear Factor-4α Gene in Japanese with Non-Insulin-Dependent Diabetes: A Nucleotide Substitution in the Polypyrimidine Tract of Intron 1b

Author

Sakurai, K., primary, Seki, N., additional, Fujii, R., additional, Yagui, K., additional, Tokuyama, Y., additional, Shimada, F., additional, Makino, H., additional, Suzuki, Y., additional, Hashimoto, N., additional, Saito, Y., additional, Egashira, T., additional, Matsui, K., additional, and Kanatsuka, A., additional

Published
2000
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240. Polar patrol balloon project in Japan

Author

Ejiri, M., primary, Nishimura, J., additional, Yajima, N., additional, Hirasawa, T., additional, Fujii, R., additional, Akiyama, H., additional, Yamagami, T., additional, Ohta, S., additional, Kanzawa, H., additional, Tohyama, F., additional, and Kokubun, S., additional

Published
1994
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243. Polar patrol balloon experiment in Antarctica

Author

Ejiri, M., primary, Kadokura, A., additional, Hirasawa, T., additional, Sato, N., additional, Fujii, R., additional, Miyaoka, H., additional, Nishimura, J., additional, Yajima, N., additional, Yamagami, T., additional, Kokubun, S., additional, Fukunishi, H., additional, Yamanaka, M., additional, and Kodama, M., additional

Published
1993
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