Search

Your search keyword '"Fujie, Toshinori"' showing total 481 results
Start Over Author "Fujie, Toshinori"
481 results on '"Fujie, Toshinori"'

204. In situ transplantation of adipose tissue‐derived stem cells organized on porous polymer nanosheets for murine skin defects.

Author

Nishiwaki, Keisuke, Aoki, Shimpo, Kinoshita, Manabu, Kiyosawa, Tomoharu, Suematsu, Yoshitaka, Takeoka, Shinji, and Fujie, Toshinori

Subjects
POROUS materials, LABORATORY mice, STEM cell transplantation, CELL proliferation, POLYSTYRENE
Abstract

Stem cell transplantation is expected to be an effective early‐phase treatment for deep burn injuries and intractable ulcers. Localizing and proliferating stem cells on the lesion utilizing engineered scaffolds is important for this treatment. In this study, we demonstrated in situ transplantation of adipose‐tissue derived stem cells (ASCs) organized on free‐standing porous polymer ultrathin films (referred to as "porous nanosheets") to a skin defect model in diabetic mice. Porous nanosheets were prepared by a combination of micro‐gravure coating with macrophase separation of poly(d,l‐lactic acid) and polystyrene under a roll‐to‐roll process and solvent etching process with cyclohexane. The permeable structure of porous nanosheets (thickness of 150 nm, average pore diameter of 4 μm) allowed for proliferation of ASCs and also provided sufficient nutrient inflow into multilayered ASC constructs. Then, transplantation of a trilayered ASC‐laden porous nanosheet achieved homogeneous transference of ASCs onto the skin lesion. Transplanted ASCs contributed to wound healing in a dorsal skin defect model in diabetic mice. Thus, cell transplantation using porous nanosheets will be a new method for promoting wound healing in diabetic and other kinds of refractory ulcers. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B: 1363–1371, 2019. [ABSTRACT FROM AUTHOR]

Published
2019
Full Text
View/download PDF

206. Stem Cell Differentiation Toward the Myogenic Lineage for Muscle Tissue Regeneration: A Focus on Muscular Dystrophy

Author

Harvard University--MIT Division of Health Sciences and Technology, Khademhosseini, Ali, Ostrovidov, Serge, Shi, Xuetao, Sadeghian, Ramin Banan, Salehi, Sahar, Fujie, Toshinori, Bae, Hojae, Ramalingam, Murugan, Harvard University--MIT Division of Health Sciences and Technology, Khademhosseini, Ali, Ostrovidov, Serge, Shi, Xuetao, Sadeghian, Ramin Banan, Salehi, Sahar, Fujie, Toshinori, Bae, Hojae, and Ramalingam, Murugan

Abstract

Skeletal muscle tissue engineering is one of the important ways for regenerating functionally defective muscles. Among the myopathies, the Duchenne muscular dystrophy (DMD) is a progressive disease due to mutations of the dystrophin gene leading to progressive myofiber degeneration with severe symptoms. Although current therapies in muscular dystrophy are still very challenging, important progress has been made in materials science and in cellular technologies with the use of stem cells. It is therefore useful to review these advances and the results obtained in a clinical point of view. This article focuses on the differentiation of stem cells into myoblasts, and their application in muscular dystrophy. After an overview of the different stem cells that can be induced to differentiate into the myogenic lineage, we introduce scaffolding materials used for muscular tissue engineering. We then described some widely used methods to differentiate different types of stem cell into myoblasts. We highlight recent insights obtained in therapies for muscular dystrophy. Finally, we conclude with a discussion on stem cell technology. We discussed in parallel the benefits brought by the evolution of the materials and by the expansion of cell sources which can differentiate into myoblasts. We also discussed on future challenges for clinical applications and how to accelerate the translation from the research to the clinic in the frame of DMD., World Premier International Research Center Initiative (WPI)

Published
2016

215. Spatial coordination of cell orientation directed by nanoribbon sheets

Author

Fujie, Toshinori, Shi, Xuetao, Ostrovidov, Serge, Liang, Xiaobin, Nakajima, Ken, Chen, Yin, Wu, Hongkai, Khademhosseini, Ali, Fujie, Toshinori, Shi, Xuetao, Ostrovidov, Serge, Liang, Xiaobin, Nakajima, Ken, Chen, Yin, Wu, Hongkai, and Khademhosseini, Ali

Abstract

Spatial coordination of cell orientation is of central importance in tissue/organ construction. In this study, we developed microfabricated poly(lactic-co-glycolic acid) (PLGA) nanoribbon sheets with unique structures, using spin-coating and micropatterning techniques, in order to generate a hierarchically assembled cellular structure consisting of murine skeletal myoblasts (C2C12). The nanoribbon sheets were composed of aligned PLGA nanoribbons in the center, and strips on four sides which take a role as bridges to connect and immobilize the aligned nanoribbons. Such unique structures facilitated the alignment of C2C12 cells into bilayer cell sheets, and cellular alignment was directed by the aligned direction of nanoribbons. The nanoribbon sheets also facilitated the construction of multilayer cell sheets with anisotropic (orthogonal) and isotropic (parallel) orientations. The enhanced expression of myogenic genes of C2C12 cells on the bilayer cell sheets demonstrated that the nanoribbons induced C2C12 cell differentiation into mature myoblasts. The micropatterned nanoribbon sheets may be a useful tool for directing cellular organization with defined alignment for regenerative medicine and drug screening applications. (C) 2015 Elsevier Ltd. All rights reserved.

Published
2015

217. The efficacy of basic fibroblast growth factor‐loaded poly(lactic‐<italic>co</italic>‐glycolic acid) nanosheet for mouse wound healing.

Author

Aoki, Shimpo, Fujii, Mao, Fujie, Toshinori, Nishiwaki, Keisuke, Miyazaki, Hiromi, Saitoh, Daizoh, Takeoka, Shinji, Kiyosawa, Tomoharu, and Kinoshita, Manabu

Subjects
ALCOHOLS (Chemical class), ANIMAL experimentation, FIBROBLASTS, GROWTH factors, LACTIC acid, MICE, NANOPARTICLES, RECOMBINANT proteins, STATISTICS, WOUND healing, DATA analysis, DATA analysis software, DESCRIPTIVE statistics
Abstract

Abstract: Although human recombinant basic fibroblast growth factor (bFGF) is widely used for wound healing, daily treatment with bFGF is required because of its short half‐life. An effective controlled‐release system of bFGF is, therefore, desired in clinical settings. To investigate the efficacy of a bFGF‐loaded nanosheet for wound healing, focusing on the controlled‐release of bFGF, bFGF‐loaded poly(lactic‐co‐glycolic acid) (PGLA) nanosheets were developed, and their in vitro release profile of bFGF and their in vivo efficacy for wound healing were examined. A polyion complex of positively charged human recombinant bFGF and negatively charged alginate was sandwiched between PLGA nanosheets (70 nm thick for each layer). The resulting bFGF‐loaded nanosheet robustly adhered to silicon skin by observation using a microscratch test. bFGF was gradually and continuously released over three days in an in vitro incubation study. Treatment with the bFGF‐loaded nanosheets (every 3 day for 15 days) as well as with a conventional bFGF spray effectively promoted wound healing of mouse dorsal skin defects with accelerated tissue granulation and angiogenesis, although the dose of bFGF used in the treatment with the bFGF nanosheets was approximately 1/20 of the sprayed bFGF. In conclusion, we developed a bFGF‐loaded nanosheet that sustained a continuous release of bFGF over three days and effectively promoted wound healing in mice. [ABSTRACT FROM AUTHOR]

Published
2017
Full Text
View/download PDF

221. Periosteum-Mimetic Structures Made from Freestanding Microgrooved Nanosheets

Author

Shi, Xuetao, Fujie, Toshinori, Saito, Akihiro, Takeoka, Shinji, Hou, Ying, Shu, Yiwei, Chen, Mingwei, Wu, Hongkai, Khademhosseini, Ali, Shi, Xuetao, Fujie, Toshinori, Saito, Akihiro, Takeoka, Shinji, Hou, Ying, Shu, Yiwei, Chen, Mingwei, Wu, Hongkai, and Khademhosseini, Ali

Abstract

A "sticker-like" PLGA nanosheet with microgrooved patterns is developed through a facile combination of spin coating and micropatterning techniques. The resulting microgrooved PLGA nanosheets can be physically adhered on flat or porous surfaces with excellent stability in aqueous environments and can harness the spatial arrangements of cells, which make it a promising candidate for generating biomimic periosteum for bone regenerative applications.

Published
2014

222. Three-dimensional co-culture of C2C12/PC12 cells improves skeletal muscle tissue formation and function

Author

Ostrovidov, Serge, primary, Ahadian, Samad, additional, Ramon-Azcon, Javier, additional, Hosseini, Vahid, additional, Fujie, Toshinori, additional, Parthiban, S. Prakash, additional, Shiku, Hitoshi, additional, Matsue, Tomokazu, additional, Kaji, Hirokazu, additional, Ramalingam, Murugan, additional, Bae, Hojae, additional, and Khademhosseini, Ali, additional

Published
2014
Full Text
View/download PDF

233. Boron Nitride Nanotube-Mediated Stimulation of Cell Co-Culture on Micro-Engineered Hydrogels

Author

Ricotti, Leonardo, primary, Fujie, Toshinori, additional, Vazão, Helena, additional, Ciofani, Gianni, additional, Marotta, Roberto, additional, Brescia, Rosaria, additional, Filippeschi, Carlo, additional, Corradini, Irene, additional, Matteoli, Michela, additional, Mattoli, Virgilio, additional, Ferreira, Lino, additional, and Menciassi, Arianna, additional

Published
2013
Full Text
View/download PDF

240. Development of a ubiquitously transferrable silver-nanoparticle-loaded polymer nanosheet as an antimicrobial coating.

Author

Ito, Keisuke, Saito, Akihiro, Fujie, Toshinori, Miyazaki, Hiromi, Kinoshita, Manabu, Saitoh, Daizoh, Ohtsubo, Shinya, and Takeoka, Shinji

Abstract

Ultra-thin polymer films (nanosheets) fabricated by a layer-by-layer (LbL) method possess unique properties such as high flexibility, adhesive strength, and transparency, and can be peeled off from a substrate and attached to various surfaces via a water-soluble supporting film. Therefore, flexible and transferrable LbL nanosheets are convenient tools as coating materials. Here, we fabricated a novel antimicrobial coating material by embedding silver nanoparticles (AgNPs) in an LbL nanosheet composed of layers of chitosan and sodium alginate (Ag-LbL nanosheet) by means of a photo-reduction method. Optimizing the amount of irradiated energy applied led to robust antimicrobial efficacy against methicillin-resistant Staphylococcus aureus (MRSA), sufficient to meet ISO standards (ISO 22196), while maintaining the flexibility and adhesive potency of the LbL nanosheet. Thus, the Ag-LbL nanosheet is a promising coating material that can provide antimicrobial efficacy to various surfaces. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 104B: 585-593, 2016. [ABSTRACT FROM AUTHOR]

Published
2016
Full Text
View/download PDF

246. Glue-Free Stacked Luminescent Nanosheets Enable High-Resolution Ratiometric Temperature Mapping in Living Small Animals

Author

Miyagawa, Takuya, Fujie, Toshinori, Ferdinandus, Vo Doan, Tat Thang, Sato, Hirotaka, and Takeoka, Shinji

Abstract

In this paper, a microthermograph, temperature mapping with high spatial resolution, was established using luminescent molecules embedded ultrathin polymeric films (nanosheets), and demonstrated in a living small animal to map out and visualize temperature shift due to animal’s muscular activity. Herein, we report super flexible and self-adhesive (no need of glue) nanothermosensor consisting of stacked two different polymeric nanosheets with thermosensitive (Eu-tris (dinaphthoylmethane)-bis-trioctylphosphine oxide: EuDT) and insensitive (Rhodamine 800) dyes being embedded. Such stacked nanosheets allow for the ratiometric thermometry, with which the undesired luminescence intensity shift due to focal drift or animal’s z-axis displacement is eliminated and the desired intensity shift solely due to the temperature shift of the sample (living muscle) can be acquired. With the stacked luminescent nanosheets, we achieved the first-ever demonstration of video filming of chronologically changing temperature-shift distribution from the rest state to the active state of the muscles in the living animal. The polymer nanosheet engineering and in vivo microthermography presented in the paper are promising technologies to microscopically explore the heat production and heat transfer in living cells, tissues, and organisms with high spatial resolution beyond what existing thermometric technologies such as infrared thermography have ever achieved.

Published
2016
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results