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210. Progress in the detection methods of antibiotic residues in animal derived food and research.

Author

FU Haiyan, LU Huanhuan, LONG Wanjun, and SHE Yuanbin

Subjects
*ANTIBIOTIC residues, *FOOD of animal origin, *FIRE detectors, *FOOD animals, *ENZYME-linked immunosorbent assay, *FOOD research
Abstract

On the basis of a brief description of the common types of antibiotics in animal derived food, the current status of antibiotic residues and hazards, focusing on antibiotic residue detection methods and research progress in animal source foods, it was pointed out that the main types of antibiotics were fluoroquinolones, β-lactams, macrolides, tetracyclines, nitrofurans, sulfonamides, amphenicols and aminoglycosides. Currently, antibiotic abuse caused serious residual problems, and the accumulation of antibiotics in the body could trigger allergic reactions, drug resistance and other symptoms. Antibiotic detection methods mainly included chromatography, spectrometry, electrochemistry, enzyme-linked immunosorbent assay (ELISA) and chemical sensing, which respectively suffered from the problems of complicated pre-treatment process, weak anti-interference ability, susceptibility to false positives, fewer types of detection, and difficulty in realising the simultaneous detection of a variety of antibiotics, and so on. Compared with other methods, chemical sensing had the advantages of simple and rapid process, obvious color change reaction, and enormous potential for application in the detection of antibiotic residues. In the future, the structural characteristics of antibiotics can be analysed in depth, and sensors detecting a variety of antibiotics simultaneously can be developed, thus further promoting the development of rapid and accurate detection of antibiotic residues in animal derived food. [ABSTRACT FROM AUTHOR]

Published
2023
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211. Estimating nitrous oxide emissions based on TN discharge from municipal wastewater treatment plants: A case study for the city of Xiamen, China.

Author

Bai, Ruolin, Jin, Lei, Fu, Haiyan, Zhuang, Mazhan, and Wei, Yi

Subjects
SEWAGE disposal plants, NITROUS oxide, CHEMICAL processes, WASTEWATER treatment, HYDROGEN-ion concentration, CHEMICAL oxygen demand
Abstract

Mitigation of nitrous oxide (N2O) emissions is of primary importance to meet the targets of reducing carbon footprints of wastewater treatment plants (WWTPs). This paper takes the N2O discharged from a case study of wastewater treatment plants as the main research object, and then develops a novel algorithm, which can accurately estimate the amount of N2O release, and then applies it to the local wastewater treatment plants. According to the results, the nitrous oxide emission flux and the emission factors (EFs) are discussed. The results include the following: (1) The total amount of N2O discharged from Xiamen wastewater treatment plants between 2018 and 2019 was 3881.29 kg and 3642.97 kg, respectively; (2) the production of N2O emissions based on the total nitrogen (TN) algorithm in the existing wastewater treatment factories in Xiamen was obtained based on the research EFs; and (3) by controlling other factors of WWTPs process such as chemical oxygen demand /nitrogen (COD)/ (N) ratio, dissolved oxygen (DO) concentration, pH value (the degree of concentration of hydrogen ions), and solids retention time (SRT) in the wastewater treatment process, selecting a secondary wastewater treatment process with an economical and reasonable approach can reduce N2O emissions in the wastewater treatment process. © 2023 Society of Chemical Industry and John Wiley & Sons, Ltd. [ABSTRACT FROM AUTHOR]

Published
2023
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229. Calponin 2 harnesses metabolic reprogramming to determine kidney fibrosis

Author

Gui, Yuan, primary, Tao, Jianling, additional, Wang, Yuanyuan, additional, Palanza, Zachary, additional, Qiao, Yi, additional, Hargis, Geneva, additional, Kreutzer, Donald L., additional, Liu, Silvia, additional, Bastacky, Sheldon I., additional, Wang, Yanlin, additional, Yu, Yanbao, additional, Fu, Haiyan, additional, and Zhou, Dong, additional

Published
2023
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236. Limonin, a natural ERK2 agonist, protects against ischemic acute kidney injury

Author

Zhou, Xianke, primary, Xiang, Yadie, additional, Li, Dier, additional, Zhong, Menghua, additional, Hong, Xue, additional, Gui, Yuan, additional, Min, Wenjian, additional, Chen, Yudan, additional, Zeng, Xi, additional, Zhu, Haili, additional, Liu, Youhua, additional, Liu, Shijia, additional, Yang, Peng, additional, Hou, Fanfan, additional, Zhou, Dong, additional, and Fu, Haiyan, additional

Published
2023
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241. Mechanisms controlling the infection of Culicoides biting midges with bluetongue virus

Author

Fu, Haiyan

Subjects
572.8, Mosquitos, African sheep disease, Molecular biology, Cytology, Genetics, Biochemistry, Zoology
Abstract

The mechanisms controlling the transmission of bluetongue virus (DTV) by vector Culicoides species were studied using immunohistochemistry, virus titration assays, in vitro transmission tests, viral binding protein analyses and transmission electron microscopy. After infection with BTV by intrathoracic (IT) inoculation, 100% of C. variipennis individuals from a susceptible colony developed a fully disseminated infection and transmitted the virus through their saliva. However only 35.4% of midges were . persistently infected after ingestion of an infectious blood meal, while only 12.1 % of persistently infected midges transmitted the virus through their saliva. The titres of BTV were about 10,·oTCIDsJmidge [Standard error of means (SEM) of log-transformed data=0.15, n=1400] in IT inoculated midges and varied from 0.32 to lQs.oTCIDsJmidge in orally infected individuals. Only those midges containing ~1 03.oTCIDso of BTV could transmit the virus through their saliva. The following patterns were observed in orally (persistently) infected individuals: 1) virus was restricted to the anterior and posterior midgut, and the foregut-midgut junction; 2) virus replicated in the gut cells, disseminated into the haemocoel but could only be detected in a few sporadic fat body cells beyond the gut; 3) virus escaped from the gut cells into the haemocoel and replicated in some secondary organs/tissues but at low levels; 4) a fully disseminated infection was observed and virus replicated in the haemocoel and secondary organs/tissues, including the salivary glands, at high levels. The infection of the gut can be divided into two main types: 1) virus replication in gut cells ranging from very low to higher levels but with virus spread throughout the cytoplasm of the infected cells; 2) virus positive reaction restricted to endosome-like structures in the cytoplasm of some gut cells. BTV was detected in the anterior and posterior midgut, foregut-midgut junction, fat body, ganglia, salivary glands and ommatidia of the compound eyes of some infected midges. No virus was ever found in the hindgut cells, muscles, Malpighian tubes and oocytes/nurse cells of the ovaries. BTV infection of the salivary glands of C. l'ariipcnnis was shown to follow a typical pattern. Virus entered the acinar cells from the haemococl passing through the basement membrane, then localised and replicated in virus inclusion bodies (VIBs) in the cytoplasm of acinar cells. Mature progeny virus particles were released into acini, then transported through intermediate ducts and accumulated in crystalline arrays in the lumen of the major secretory ducts. No virus was released back into the haemocoel through the basement membrane; nor was virus released back into acinar cells from the acini. Nervous tissue of C. l'ariipennis is one of the most susceptible tissues to BTV. Ultrastructural observation showed characteristics ofBTV replication, including formation of VIBs, large amounts of progeny virus particles and tubules, in infected thoracic ganglia. A 60-kD viral protein adhered to both BHK-21 (mammalian) cells and a Culicoides cell line, KC cells. A 44-kD BTV viral protein, co-migrating with non structural protein NS2, adsorbed to BHK-21 cells but not to KC cells, while a 39.6 kD viral protein, co-migrating with major inner capsid protein VP7, adhered only to KC cells but not to BHK-21 cells.

Published
1995

245. Adomian Decomposition, Firing Change Process Analysis and Synchronous Control of Fractional-Order Hindmarsh–Rose Neurons in Electromagnetic Field.

Author

Lei, Tengfei, Fu, Haiyan, Zang, Hongyan, Huang, Lili, and Sun, Wenqiang

Subjects
ELECTROMAGNETIC fields, SLIDING mode control, BIFURCATION diagrams, CHAOS synchronization, NEURONS
Abstract

In this paper, based on integer-order Hindmarsh–Rose (HR) neurons under an electric field, the fractional-order model is constructed, and the nonlinear term is decomposed by the Adomian decomposition method, and the numerical solution of the system is obtained. The firing behavior of the neuron model is analyzed by using a phase diagram, interspike interval (ISI) bifurcation diagram, sample entropy (SE) complexity, and largest Lyapunov exponent (LLE). Based on the sliding mode control theory, a chaos synchronization controller of the system is designed. Matlab simulation results show that the controller is realizable and effective, and also has the characteristic of fast response, which provides a reference for the control and application of a memristor neural network system. [ABSTRACT FROM AUTHOR]

Published
2023
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249. Cover Image

Author

Zhou, Li, primary, Ma, Yue, additional, Yao, Jiaxu, additional, Zhang, Minghao, additional, Fu, Haiyan, additional, Yang, Jian, additional, Liu, Jikai, additional, Zhao, Minjie, additional, and Marchioni, Eric, additional

Published
2022
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