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1,222 results on '"Friml, Jiří"'

201. Auxin-Regulated Reversible Inhibition of TMK1 Signaling by MAKR2 Modulates the Dynamics of Root Gravitropism

Author

Marquès-Bueno, Maria Mar, primary, Armengot, Laia, additional, Noack, Lise C., additional, Bareille, Joseph, additional, Rodriguez, Lesia, additional, Platre, Matthieu Pierre, additional, Bayle, Vincent, additional, Liu, Mengying, additional, Opdenacker, Davy, additional, Vanneste, Steffen, additional, Möller, Barbara K., additional, Nimchuk, Zachary L., additional, Beeckman, Tom, additional, Caño-Delgado, Ana I., additional, Friml, Jiří, additional, and Jaillais, Yvon, additional

Published
2021
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207. Proteome-wide cellular thermal shift assay reveals unexpected cross-talk between brassinosteroid and auxin signaling.

Author

Qing Lu, Yonghong Zhang, Hellner, Joakim, Giannini, Caterina, Xiangyu Xu, Pauwels, Jarne, Qian Ma, Wim Dejonghe, Huibin Han, Van de Cotte, Brigitte, Impens, Francis, Gevaert, Kris, De Smet, Ive, Friml, Jiří, Molina, Daniel Martinez, and Russinova, Eugenia

Subjects
GLYCOGEN synthase kinase, AUXIN, BIOCHEMICAL genetics, SMALL molecules, COMMERCIAL products, POST-translational modification, CHEMICAL plants
Abstract

Despite the growing interest in using chemical genetics in plant research, small molecule target identification remains a major challenge. The cellular thermal shift assay coupled with highresolution mass spectrometry (CETSA MS) that monitors changes in the thermal stability of proteins caused by their interactions with small molecules, other proteins, or posttranslational modifications, allows the discovery of drug targets or the study of protein-metabolite and protein-protein interactions mainly in mammalian cells. To showcase the applicability of this method in plants, we applied CETSA MS to intact Arabidopsis thaliana cells and identified the thermal proteome of the plant-specific glycogen synthase kinase 3 (GSK3) inhibitor, bikinin. A comparison between the thermal and the phosphoproteomes of bikinin revealed the auxin efflux carrier PIN-FORMED1 (PIN1) as a substrate of the Arabidopsis GSK3s that negatively regulate the brassinosteroid signaling. We established that PIN1 phosphorylation by the GSK3s is essential for maintaining its intracellular polarity that is required for auxin-mediated regulation of vascular patterning in the leaf, thus revealing cross-talk between brassinosteroid and auxin signaling. [ABSTRACT FROM AUTHOR]

Published
2022
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213. Receptor kinase module targets PIN-dependent auxin transport during canalization

Author

Hajný, Jakub, primary, Prát, Tomáš, additional, Rydza, Nikola, additional, Rodriguez, Lesia, additional, Tan, Shutang, additional, Verstraeten, Inge, additional, Domjan, David, additional, Mazur, Ewa, additional, Smakowska-Luzan, Elwira, additional, Smet, Wouter, additional, Mor, Eliana, additional, Nolf, Jonah, additional, Yang, BaoJun, additional, Grunewald, Wim, additional, Molnár, Gergely, additional, Belkhadir, Youssef, additional, De Rybel, Bert, additional, and Friml, Jiří, additional

Published
2020
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215. Salicylic acid regulates PIN2 auxin transporter hyperclustering and root gravitropic growth via Remorin ‐dependent lipid nanodomain organisation in Arabidopsis thaliana

Author

Ke, Meiyu, primary, Ma, Zhiming, additional, Wang, Deyan, additional, Sun, Yanbiao, additional, Wen, Chenjin, additional, Huang, Dingquan, additional, Chen, Zichen, additional, Yang, Liang, additional, Tan, Shutang, additional, Li, Ruixi, additional, Friml, Jiří, additional, Miao, Yansong, additional, and Chen, Xu, additional

Published
2020
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216. Endocytosis of BRASSINOSTEROID INSENSITIVE1 Is Partly Driven by a Canonical Tyr-Based Motif

Author

Liu, Derui, primary, Kumar, Rahul, additional, Claus, Lucas A.N., additional, Johnson, Alexander J., additional, Siao, Wei, additional, Vanhoutte, Isabelle, additional, Wang, Peng, additional, Bender, Kyle W., additional, Yperman, Klaas, additional, Martins, Sara, additional, Zhao, Xiuyang, additional, Vert, Grégory, additional, Van Damme, Daniël, additional, Friml, Jiří, additional, and Russinova, Eugenia, additional

Published
2020
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217. Cellular requirements for PIN polar cargo clustering in Arabidopsis thaliana

Author

Li, Hongjiang, primary, Wangenheim, Daniel, additional, Zhang, Xixi, additional, Tan, Shutang, additional, Darwish‐Miranda, Nasser, additional, Naramoto, Satoshi, additional, Wabnik, Krzysztof, additional, De Rycke, Riet, additional, Kaufmann, Walter A., additional, Gütl, Daniel, additional, Tejos, Ricardo, additional, Grones, Peter, additional, Ke, Meiyu, additional, Chen, Xu, additional, Dettmer, Jan, additional, and Friml, Jiří, additional

Published
2020
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218. Pharmacological and genetic manipulations of Ca2+ signaling have contrasting effects on auxin-regulated trafficking

Author

Wang, Ren, primary, Himschoot, Ellie, additional, Grenzi, Matteo, additional, Chen, Jian, additional, Krebs, Melanie, additional, Schumacher, Karin, additional, Nowack, Moritz K., additional, Van Damme, Daniël, additional, De Smet, Ive, additional, Beeckman, Tom, additional, Costa, Alex, additional, Friml, Jiří, additional, and Vanneste, Steffen, additional

Published
2020
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220. Mutually opposing activity of PIN7 splicing isoforms is required for auxin-mediated tropic responses in Arabidopsis thaliana

Author

Kashkan, Ivan, primary, Hrtyan, Mónika, additional, Retzer, Katarzyna, additional, Humpolíčková, Jana, additional, Jayasree, Aswathy, additional, Filepová, Roberta, additional, Vondráková, Zuzana, additional, Simon, Sibu, additional, Rombaut, Debbie, additional, Jacobs, Thomas B., additional, Frilander, Mikko J., additional, Hejátko, Jan, additional, Friml, Jiří, additional, Petrášek, Jan, additional, and Růžička, Kamil, additional

Published
2020
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224. Arabidopsis Flippases Cooperate with ARF GTPase Exchange Factors to Regulate the Trafficking and Polarity of PIN Auxin Transporters

Author

Zhang, Xixi, primary, Adamowski, Maciek, additional, Marhava, Petra, additional, Tan, Shutang, additional, Zhang, Yuzhou, additional, Rodriguez, Lesia, additional, Zwiewka, Marta, additional, Pukyšová, Vendula, additional, Sánchez, Adrià Sans, additional, Raxwal, Vivek Kumar, additional, Hardtke, Christian S., additional, Nodzyński, Tomasz, additional, and Friml, Jiří, additional

Published
2020
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225. Rare earth elements induce cytoskeleton-dependent and PI4P-associated rearrangement of SYT1/SYT5 endoplasmic reticulum–plasma membrane contact site complexes in Arabidopsis

Author

Lee, EunKyoung, primary, Santana, Brenda Vila Nova, primary, Samuels, Elizabeth, primary, Benitez-Fuente, Francisco, primary, Corsi, Erica, primary, Botella, Miguel A, primary, Perez-Sancho, Jessica, primary, Vanneste, Steffen, primary, Friml, Jiří, primary, Macho, Alberto, primary, Azevedo, Aristea Alves, primary, and Rosado, Abel, primary

Published
2020
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229. Colonization of endophyte Acremonium sp. D212 in Panax notoginseng and rice mediated by auxin and jasmonic acid

Author

Han, Li, primary, Zhou, Xuan, additional, Zhao, Yiting, additional, Zhu, Shusheng, additional, Wu, Lixia, additional, He, Yunlu, additional, Ping, Xiangrui, additional, Lu, Xinqi, additional, Huang, Wuying, additional, Qian, Jie, additional, Zhang, Lina, additional, Jiang, Xi, additional, Zhu, Dan, additional, Luo, Chongyu, additional, Li, Saijie, additional, Dong, Qian, additional, Fu, Qijing, additional, Deng, Kaiyuan, additional, Wang, Xin, additional, Wang, Lei, additional, Peng, Sheng, additional, Wu, Jinsong, additional, Li, Weimin, additional, Friml, Jiří, additional, Zhu, Youyong, additional, He, Xiahong, additional, and Du, Yunlong, additional

Published
2020
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231. Salicylic Acid Targets Protein Phosphatase 2A to Attenuate Growth in Plants

Author

Tan, Shutang, primary, Abas, Melinda, additional, Verstraeten, Inge, additional, Glanc, Matouš, additional, Molnár, Gergely, additional, Hajný, Jakub, additional, Lasák, Pavel, additional, Petřík, Ivan, additional, Russinova, Eugenia, additional, Petrášek, Jan, additional, Novák, Ondřej, additional, Pospíšil, Jiří, additional, and Friml, Jiří, additional

Published
2020
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239. Subcellular homeostasis of phytohormone auxin is mediated by the ER-localized PIN5 transporter

Author

Mravec, Jozef, Skůpa, Petr, Bailly, Aurélien, Hoyerová, Klára, Křeček, Pavel, Bielach, Agnieszka, Petrášek, Jan, Zhang, Jing, Gaykova, Vassilena, Stierhof, York-Dieter, Dobrev, Petre I., Schwarzerová, Kateřina, Rolčík, Jakub, Seifertová, Daniela, Luschnig, Christian, Benková, Eva, Zažímalová, Eva, Geisler, Markus, and Friml, Jiří

Published
2009
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241. Mutually opposing activity of PIN7 splicing isoforms is required for auxin‐mediated tropic responses in Arabidopsis thaliana.

Author

Kashkan, Ivan, Hrtyan, Mónika, Retzer, Katarzyna, Humpolíčková, Jana, Jayasree, Aswathy, Filepová, Roberta, Vondráková, Zuzana, Simon, Sibu, Rombaut, Debbie, Jacobs, Thomas B., Frilander, Mikko J., Hejátko, Jan, Friml, Jiří, Petrášek, Jan, and Růžička, Kamil

Subjects
TROPISMS, ARABIDOPSIS thaliana, CELL membranes, GENETIC engineering, PLANT development
Abstract

Summary: Advanced transcriptome sequencing has revealed that the majority of eukaryotic genes undergo alternative splicing (AS). Nonetheless, little effort has been dedicated to investigating the functional relevance of particular splicing events, even those in the key developmental and hormonal regulators.Combining approaches of genetics, biochemistry and advanced confocal microscopy, we describe the impact of alternative splicing on the PIN7 gene in the model plant Arabidopsis thaliana. PIN7 encodes a polarly localized transporter for the phytohormone auxin and produces two evolutionarily conserved transcripts, PIN7a and PIN7b.PIN7a and PIN7b, differing in a four amino acid stretch, exhibit almost identical expression patterns and subcellular localization. We reveal that they are closely associated and mutually influence each other's mobility within the plasma membrane. Phenotypic complementation tests indicate that the functional contribution of PIN7b per se is minor, but it markedly reduces the prominent PIN7a activity, which is required for correct seedling apical hook formation and auxin‐mediated tropic responses.Our results establish alternative splicing of the PIN family as a conserved, functionally relevant mechanism, revealing an additional regulatory level of auxin‐mediated plant development. [ABSTRACT FROM AUTHOR]

Published
2022
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242. Transcriptional Analysis in the Arabidopsis Roots Reveals New Regulators that Link rac-GR24 Treatment with Changes in Flavonol Accumulation, Root Hair Elongation and Lateral Root Density.

Author

Struk, Sylwia, Braem, Lukas, Matthys, Cedrick, Walton, Alan, Vangheluwe, Nick, Praet, Stan Van, Jiang, Lingxiang, Baster, Pawel, Cuyper, Carolien De, Boyer, François-Didier, Stes, Elisabeth, Beeckman, Tom, Friml, Jiří, Gevaert, Kris, and Goormachtig, Sofie

Subjects
FLAVONOLS, TRANSCRIPTION factors, CELLULAR signal transduction, DENSITY, TRANSCRIPTOMES
Abstract

The synthetic strigolactone (SL) analog, rac -GR24, has been instrumental in studying the role of SLs as well as karrikins because it activates the receptors DWARF14 (D14) and KARRIKIN INSENSITIVE 2 (KAI2) of their signaling pathways, respectively. Treatment with rac -GR24 modifies the root architecture at different levels, such as decreasing the lateral root density (LRD), while promoting root hair elongation or flavonol accumulation. Previously, we have shown that the flavonol biosynthesis is transcriptionally activated in the root by rac -GR24 treatment, but, thus far, the molecular players involved in that response have remained unknown. To get an in-depth insight into the changes that occur after the compound is perceived by the roots, we compared the root transcriptomes of the wild type and the more axillary growth2 (max2) mutant, affected in both SL and karrikin signaling pathways, with and without rac -GR24 treatment. Quantitative reverse transcription (qRT)-PCR, reporter line analysis and mutant phenotyping indicated that the flavonol response and the root hair elongation are controlled by the ELONGATED HYPOCOTYL 5 (HY5) and MYB12 transcription factors, but HY5, in contrast to MYB12, affects the LRD as well. Furthermore, we identified the transcription factors TARGET OF MONOPTEROS 5 (TMO5) and TMO5 LIKE1 as negative and the Mediator complex as positive regulators of the rac -GR24 effect on LRD. Altogether, hereby, we get closer toward understanding the molecular mechanisms that underlay the rac -GR24 responses in the root. [ABSTRACT FROM AUTHOR]

Published
2022
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243. Cell surface and intracellular auxin signalling for H+ fluxes in root growth.

Author

Li, Lanxin, Verstraeten, Inge, Roosjen, Mark, Takahashi, Koji, Rodriguez, Lesia, Merrin, Jack, Chen, Jian, Shabala, Lana, Smet, Wouter, Ren, Hong, Vanneste, Steffen, Shabala, Sergey, De Rybel, Bert, Weijers, Dolf, Kinoshita, Toshinori, Gray, William M., and Friml, Jiří

Abstract

Growth regulation tailors development in plants to their environment. A prominent example of this is the response to gravity, in which shoots bend up and roots bend down1. This paradox is based on opposite effects of the phytohormone auxin, which promotes cell expansion in shoots while inhibiting it in roots via a yet unknown cellular mechanism2. Here, by combining microfluidics, live imaging, genetic engineering and phosphoproteomics in Arabidopsis thaliana, we advance understanding of how auxin inhibits root growth. We show that auxin activates two distinct, antagonistically acting signalling pathways that converge on rapid regulation of apoplastic pH, a causative determinant of growth. Cell surface-based TRANSMEMBRANE KINASE1 (TMK1) interacts with and mediates phosphorylation and activation of plasma membrane H+-ATPases for apoplast acidification, while intracellular canonical auxin signalling promotes net cellular H+ influx, causing apoplast alkalinization. Simultaneous activation of these two counteracting mechanisms poises roots for rapid, fine-tuned growth modulation in navigating complex soil environments.Auxin rapidly modulates root growth through simultaneous activation of two opposing mechanisms—TMK1-mediated apoplast acidification and TIR1/AFB-mediated apoplast alkalinization. [ABSTRACT FROM AUTHOR]

Published
2021
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246. Auxin guides roots to avoid obstacles during gravitropic growth

Author

Zhang, Yuzhou and Friml, Jiří

Subjects
Indoleacetic Acids, Forum, Arabidopsis Proteins, plant evolution, Arabidopsis, Biological Transport, Plants, Genetically Modified, Plant Roots, PIN auxin transporter, obstacle avoidance, Gravitropism, thigmotropism, Seedlings, Commentary, Calcium Signaling, auxin
Abstract

Plants sense mechanical stimuli to recognise nearby obstacles and change their growth patterns to adapt to the surrounding environment. When roots encounter an obstacle, they rapidly bend away from the impenetrable surface and find the edge of the barrier. However, the molecular mechanisms underlying root-obstacle avoidance are largely unknown. Here, we demonstrate that PIN-FORMED (PIN)-mediated polar auxin transport facilitates root bending during obstacle avoidance. We analysed two types of bending after roots touched barriers. In auxin receptor mutants, the rate of root movement during first bending was largely delayed. Gravity-oriented second bending was also disturbed in these mutants. The reporter assays showed that asymmetrical auxin responses occurred in the roots during obstacle avoidance. Pharmacological analysis suggested that polar auxin transport mediates local auxin accumulation. We found that PINs are required for auxin-assisted root bending during obstacle avoidance. We propose that rapid root movement during obstacle avoidance is not just a passive but an active bending completed through polar auxin transport. Our findings suggest that auxin plays a role in thigmotropism during plant-obstacle interactions.

Published
2019

247. Nitrate Modulates the Differentiation of Root Distal Stem Cells1[OPEN]

Author

Wang, Yalu, Gong, Zhizhong, Friml, Jiří, and Zhang, Jing

Subjects
Nitrates, Indoleacetic Acids, Nitrogen, fungi, Meristem, Arabidopsis, food and beverages, heterocyclic compounds, Cell Differentiation, Letters
Abstract

Nitrate regulation of root stem cell activity is auxin-dependent.

Published
2019

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