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ABSTRACT: The objective of this study was to investigate the effects of an anti-coccidiosis drug, vaccine, probiotic, symbiotic, and phytochemicals in the prevention and control of coccidia infection in broilers. A total of 525 one-day-old Ross 308 chicks were randomly allocated to 7 experimental diets with 5 replicates of 15 birds each in a completely randomized design. Experimental diets consisted of negative control (NC) without any additives and not challenged. The other 6 groups were challenged with mixed Eimeria and fed the basal diet with no additives (Positive Control, PC) or supplemented with Coxidine 100 (1 g / 1 kg), probiotic, synbiotic, Livacox T vaccine and phytobiotic additives based on the manufacturer's recommended dose. Body weight gain (WG), feed intake (FI) and feed conversion ratio (FCR) were recorded weekly. Oocysts per gram of excreta (OPG) were determined on d 25 to 33 and 42. One bird per cage was euthanized to analyze lesion score and jejunum and ileum inflammatory genes expression. Coccidial challenge reduced WG (P < 0.05) during 15 to 28 d and vaccine treatment was more effective in improving WG and FCR on d 29 to 42 and 1 to 42 (P < 0.05) than other treatments. Birds in the PC group had higher (P < 0.05) OPG than NC group for all days and the vaccine treatment resulted in the lowest rate of OPG compared to other treatments (P < 0.05) at 27, 28, 29, 30, 32, and 33 d of age and overall average. Relative mRNA levels of IFN-γ, IL-1β and IL-10 were significantly upregulated among treatments under coccidiosis challenge in jejunum and ileum except for IL-1β expression in the ileum. In conclusion, based on the results of this study the individual characteristics of feed additives for the prevention of coccidiosis can be different depending on the type and source of feed additives, duration, and amount used, levels of oocyst inoculation and Eimeria types.

The term ‘tea polyphenols’ (TPs) comprises a broad range of polyphenol compounds present in tea, demonstrating positive health effects such as antioxidant activity, etc. in animals. This study investigated the effects of TPs supplementation in juvenile genetically improved farmed tilapia (GIFT); Oreochromis niloticus). Fifteen fish (weight: 13.46 ± 1.25 g) were distributed in six tanks and randomly assigned with diets containing graded levels of TPs (mg/kg): 0.00 (control), 100, 200, 300, 400 and 500 in quadruplicate for 8 weeks. After the trial, the fish fed 500 mg/kg TPs exhibited a significant reduction (p

The widespread used organoarsenicals have drawn attention for decades due to their potential environment risks. In this study, a heterogeneous system of goethite/oxalate irradiated using UVA light (λ = 365 nm) was applied for the removal of ASA, a kind of organoarsenicals used in animal feeding operations as additives, from the aqueous phase through photodegradation. Results showed that the presence of 5 mM of oxalate significantly enhanced the photodegradation efficiency of ASA in the 0.1 g/L of goethite suspended system from 28 to ~100% within 180 min reaction at pH 5. Acid conditions favored the photoreaction rate, compared with neutral and basic conditions. This reaction process was also influenced by the initial concentration of oxalate and ASA. Furthermore, the mechanism study was conducted by quenching experiments and revealed the important roles of ·OH in the degradation of ASA in the goethite/oxalate/UVA system. By analyzing the reaction products, both inorganic arsenic (As(III) and As(V)) and ammonia were detected during the photodegradation of ASA. These findings help to gain a better understanding of the geochemical behavior of ASA in surface water and can also provide a potential treatment method for the organoarsenicals contaminated water. [ABSTRACT FROM AUTHOR]

Postbiotics has become a new hotspot in the field of microbial preparations, which has positive functions such as maintenance of intestinal health, improvement of growth performance, enhancement of host immunity and tolerance to stress. The article expounded the main mechanism of postbiotics, and summarized the application of postbiotics in aquaculture. The review aims to promote the development and application of postbiotics products in aquaculture industry. [ABSTRACT FROM AUTHOR]

Simple Summary: Porcine epidemic diarrhea (PED) is caused by a coronavirus that poses a serious threat to the global pig industry. Black soldier fly is a very high-quality protein raw material that could serve as a functional feed additive to improve intestinal health in piglets. The study aimed to evaluate the effects of black soldier fly extract (BFE) on growth performance, plasma biochemical parameters, antioxidant capacity and antiviral genes in PEDV-infected piglets. The results showed that the addition of 500 mg/kg BW BFE to the diet could improve the intestinal function of piglets and activate the immune system after PEDV infection. Porcine epidemic diarrhea virus (PEDV) has developed as a global problem for the pig business, resulting in significant financial losses. Black soldier fly extract (BFE) has been proven to improve intestinal growth in pigs after weaning. Consequently, the goal of the present investigation was to explore the effects of BFE supplementation on intestinal function in PEDV-infected piglets. Eighteen piglets were randomly allocated to three groups: control, PEDV, and BFE + PEDV. The piglets in the BFE + PEDV group received 500 mg/kg BW of BFE orally for seven days from day 4 to 10 of the study. On day 9 of the study, six pigs from each group received either clean saline or PEDV solution at a dosage of 106 TCID50 (50% tissue culture infectious dose) per pig. On day 11, samples of blood and intestine were taken for additional investigation. The results indicated a significant decrease in the average daily gain (ADG) of piglets infected with PEDV (p < 0.05). Additionally, PEDV infection led to an alteration of blood indexes and a reduction in plasma D-xylose concentration and villi height in the small intestine, while it increased plasma diamine oxidase activity and small intestinal crypt depth in piglets (p < 0.05). The PEDV infection significantly reduced antioxidant enzyme activity in plasma and the gut, including total superoxide dismutase and catalase, while increasing contents of oxidation-relevant products such as malondialdehyde and hydrogen peroxide in piglets. Moreover, PEDV infection increased the mRNA expression level of antiviral-related genes (p < 0.05). Nutritional supplementation with BFE improved intestinal histomorphological indicators and reduced oxidative stress produced by PEDV infection in piglets. Interestingly, BFE could significantly promote the mRNA expression level of antiviral-related genes in the ileum (p < 0.05). Overall, the preliminary results suggest that dietary BFE could improve intestinal function in piglets after PEDV infection. Currently, the findings put a spotlight on the role of BFE in the prevention and treatment of PED in piglets. [ABSTRACT FROM AUTHOR]

This study evaluated the effect of free and nanoencapsulated rosemary essential oil (REO) as an antibiotic alternative in broiler diets on growth performance, nutrient digestibility, carcass traits, meat quality and gene expression. Four hundred twenty day-old commercial broiler chicks (VENCOBB) were randomly allocated to seven dietary treatments, each having four replicates of fifteen chicks. The dietary treatments comprised control (CON) fed a basal diet only, AB (basal diet + 10 mg enramycin/kg), CS (basal diet + 150 mg chitosan nanoparticles/kg), REOF100 and REOF200 (basal diet + 100 mg and 200 mg free REO/kg, respectively), and REON100 and REON200 (basal diet + 100 mg and 200 mg nanoencapsulated REO/kg, respectively). Overall (7–42 d), REON200 showed the highest (p < 0.001) body weight gain (1899 g/bird) and CON had the lowest gain (1742 g/bird), while the CS, REOF100 and REOF200 groups had a similar gain, but lower than that of the AB and REON100 groups. Feed intake was not affected by dietary treatments. Overall, the feed efficiency increased (p = 0.001) by 8.47% in the REON200 group and 6.21% in the AB and REON100 groups compared with the CON. Supplementation of REO improved (p < 0.05) dry matter and crude protein digestibility, with the highest values in REON100 and REON200. Ether extract, crude fiber, calcium and phosphorus digestibility values showed no difference among the groups. The dressing, breast, thigh % increased (p < 0.05) and abdominal fat % decreased (p < 0.001) more in the REON200 group than with other treatments and CON. In breast meat quality, water holding capacity and extract reserve volume increased (p < 0.05) while drip loss and cholesterol content decreased (p < 0.05) in REON100 and REON200. No change was observed in the breast meat color among dietary treatments and CON. The REON100 and REON200 groups had reduced (p < 0.05) meat lipid peroxidation as depicted by the decreased levels of TBARS, free fatty acids and peroxide value compared to other treatments and CON. The expression of the Mucin 2, PepT1 and IL-10 genes was upregulated (p < 0.001) and TNF-α downregulated (p < 0.001) by dietary addition of REO particularly in the nanoencapsulated form compared with the CON. In conclusion, nanoencapsulated REO, especially at 200 mg/kg diet, showed promising results as an antibiotic alternative in improving the performance, nutrient digestibility, carcass traits, meat quality and upregulation of growth and anti-inflammatory genes. [ABSTRACT FROM AUTHOR]

Aquaculture serves as a source of protein and livelihood and is an alternative to capture fisheries, thereby reducing pressure on the wild. However, aquaculture tends to be limited by sustainability issues, which include overdependency on fishmeal, the high cost associated with fishmeal, the environmental impact of aquaculture activities, which may be detrimental to aquatic lives and the environment, and the use of antibiotics to treat diseases, which may have an adverse effect in their host or the environment. Efforts are being made toward attaining practical ways to enhance aquaculture sustainability. One such effort is using functional feed additives in feed formulation. Functional feed additives are dietary ingredients incorporated in feed formulations, not only for the usual provision of basic nutritional requirements as offered by traditional feed but also for growth and health enhancement; environmental and economic gain. This review emphasizes the importance of incorporating functional feed additives such as probiotics, prebiotics, symbiotics, and phytogenics. This study evaluates and presents holistic information on functional additives, their roles in enhancing aquaculture sustainability, and the challenges encountered in their application. [ABSTRACT FROM AUTHOR]

Context: Brazilian red propolis stands out for its diverse composition, which includes phenolic compounds whose effects may be similar to those of synthetic feed additives. Aims: The objective of this study was to evaluate the effects of red propolis extract on the carcass and meat characteristics of lambs finished under confinement conditions. Methods: The meat quality characteristics of the longissimus lumborum muscle were evaluated in 35 Santa Inês lambs, with an average age of 5 months and an initial average body weight of 17.08 ± 2.36 kg (mean ± s.d.), submitted to five distinct treatments (0, 7, 14, 21, 28 mL of red propolis extract/animal.day) over 68 days. Key results: The use of propolis did not affect (P > 0.05) the characteristics of the carcass, as well as the chemical composition and cholesterol content. Among the physico-chemical parameters of meat, only the shear force had a quadratic effect (P < 0.05). There was also an exponential effect for lipid oxidation (P < 0.05), where the treatment with propolis had greater antioxidant effect. Meat with a lower content (P < 0.05) of saturated fatty acids was observed in sheep that received extract. There was also an increase (P < 0.05) in the content of monounsaturated acids and consequently an improvement in nutritional quality, atherogenicity and thrombogenicity indexes of desirable fatty acids. Conclusions: The red propolis extract improved the lipid profile of the lamb meat. We recommend the inclusion of 7 mL red propolis extract/animal.day (or 59.50 mg total flavoloids/animal.day) in the diet of lambs finished under confinement conditions. Implications: The red propolis extract proved to be a viable alternative as a natural feed additive. Red propolis extract was offered at increasing levels to feedlot lambs and we observed that the animals' carcass weight and physico-chemical parameters of the meat were not influenced by these treatments. Furthermore, the inclusion of 7 mL/day of extract in lamb diet was enough to reduce the oxidation of lamb meat, and also improved the lipid profile. We recommend the inclusion of 7 mL/day of red propolis extract in the diet of feedlot lambs. [ABSTRACT FROM AUTHOR]

The article focuses on recent European Union regulations published in the Official Journal, covering amendments and renewals related to animal products, feed additives, and active substances. Topics include updates to lists authorizing the entry of consignments from third countries, extensions of approval periods for various substances, and authorizations and renewals of feed additives for animal species.

Heifers (n = 40) were randomly allocated to 5 treatment groups: (1) control (no additives); (2) virginiamycin (VM; 200 mg/d); (3) monensin (MT; 200 mg/d) + tylosin (110 mg/d); (4) monensin (MLY; 220 mg/d) + live yeast (5.0 × 108 cfu/d); (5) sodium bicarbonate (BUF; 200 g/d) + magnesium oxide (30 g/d). [ABSTRACT FROM AUTHOR]

Seabuckthorn is rich in polysaccharides, flavonoids, tannins, terpenoids, fatty acids, vitamins, and other active components. It has biological functions such as antioxidant, antibacterial, anti-inflammatory, anti-tumor, liver-protecting, and cholagogic, which can enhance the immunity and antioxidant function of livestock and poultry, improve growth performance, and improve the quality of livestock and poultry products. Adding seabuckthorn residue, seeds, leaves or extracts to diet can promote the production of pigs, sheep, dairy cows and broilers to varying degrees, reduce feed costs and improve the economic benefits of breeding, but the active components and molecular mechanisms of seabuckthorn promoting livestock and poultry production are unclear. In this paper, the active components, biological functions, and application of seabuckthorn in livestock and poultry production were summarized, which provided a reference for the mechanism research, production application and development of seabuckthorn promoting livestock and poultry production. [ABSTRACT FROM AUTHOR]

Yucca belongs to the Yucca genus of the Agave family and is a versatile plant that is edible, medicinal, and ornamental. Yucca extract contains rich saponins, polyphenols and polysaccharides as well as other active ingredients. As a natural, green and safe new feed additive, Yucca extract can fulfill a variety of biological functions in animal production, such as promoting growth, improving reproductive performance, improving feed conversion, regulating the gastrointestinal environment, improving the breeding environment, etc., and has good application prospects in the field of animal husbandry. The article introduces the main active ingredients, chemical structure, biological functions of Yucca extract and its application in animal production, and discusses the possible problems of Yucca extract feed additives, to provide references for further research and application of Yucca extract in animal production. [ABSTRACT FROM AUTHOR]

Humate may be a valuable livestock feed additive, with potential effects on nutrient utilisation and animal performance. Thus, the aim of this study was to investigate the effect of K Humate S 100R supplementation on the feed intake, liveweight gain, and carcass parameters of Angus steers. Within individual pens, 40 weaned steers were allocated to four treatment groups (n = 10/potassium humate K Humate S100R, Omnia Specialities Australia) for 100 days. The treatment groups included Group 1, 35 g K Humate S100R/animal/day; Group 2, 70 g K Humate S100R/animal/day; Group 3, 140 g K Humate S100R/animal/day; and Control Group, which were not supplemented with K Humate S100R (0 g K Humate S100R/animal/day). Chemical and mineral composition of the feed ingredients, dry matter intake (DMI), and average daily weight gains were recorded. The steers were slaughtered as a single group at a commercial Australian abattoir. Standard measures for hot standard carcass weight, eye muscle area, fat depth and coverage, marbling, ossification, meat and fat colour, dressing percentage and loin pH values at 24‐hour postmortem were recorded. It was found that the steers allocated to Group 2 had higher DMI (P = 0.003) and feed conversion ratio (FCR) (P < 0.001) compared with those allocated to Group 1 and the Control Group. The MSA marbling score was lowest for steers allocated to the Control Group (P < 0.05) and comparable for those allocated to Groups 1, 2, and 3. Together, these results demonstrate that increased levels of K Humate S100R supplementation improved the carcass quality, via an increase in MSA. However, further research is warranted on the potential effects of humates supplementation on intramuscular fat associated qualities of beef. [ABSTRACT FROM AUTHOR]

Xylanases from glycoside hydrolase (GH) families 10 and 11 are common feed additives for broiler chicken diets due to their catalytic activity on the nonstarch polysaccharide xylan. This study investigated the potential of an optimized binary GH10 and GH11 xylanase cocktail to mitigate the antinutritional effects of xylan on the digestibility of locally sourced chicken feed. Immunofluorescence visualization of the activity of the xylanase cocktail on xylan in the yellow corn of the feed showed a substantial collapse in the morphology of cell walls. Secondly, the reduction in the viscosity of the digesta of the feed by the cocktail showed an effective degradation of the soluble fraction of xylan. Analysis of the xylan degradation products from broiler feeds by the xylanase cocktail showed that xylotriose and xylopentaose were the major xylooligosaccharides (XOS) produced. In vitro evaluation of the prebiotic potential of these XOS showed that they improved the growth of the beneficial bacteria Streptococcus thermophilus and Lactobacillus bulgaricus. The antibacterial activity of broths from XOS‐supplemented probiotic cultures showed a suppressive effect on the growth of the extraintestinal infectious bacterium Klebsiella pneumoniae. Supplementing the xylanase cocktail in cereal animal feeds attenuated xylan's antinutritional effects by reducing digesta viscosity and releasing entrapped nutrients. Furthermore, the production of prebiotic XOS promoted the growth of beneficial bacteria while inhibiting the growth of pathogens. Based on these effects of the xylanase cocktail on the feed, improved growth performance and better feed conversion can potentially be achieved during poultry rearing. [ABSTRACT FROM AUTHOR]

Phytogenic feed additives (PFAs) often referred to as phytobiotics or botanical feed additives, are natural compounds derived from various plants, herbs, spices and other botanical sources. These feed additives are intended to serve a variety of purposes, including an immune system regulator, an antimicrobial, an antimutagenic, an antioxidant and a growth promoter. They are composed of bioactive compounds extracted from plants, including essential oils, polyphenols, terpenoids and flavonoids. They are mostly utilized as substitute antibiotic growth promoters in nonruminant (swine and poultry) livestock production, owing to the prohibition of antibiotic usage in the feed industry. It has been thoroughly examined to ascertain their impact on intestinal health and activity, correlation with animals' effective health and well‐being, productivity, food security and environmental impact. The potential uses of these feed additives depend on the properties of herbs, the comprehension of their principal and secondary components, knowledge of their mechanisms of action, the safety of animals and the products they produce. They are gaining recognition as effective and sustainable tools for promoting animal health and performance while reducing the reliance on antibiotics in nonruminant nutrition. Their natural origins, multifaceted benefits and alignment with consumer preferences make them a valuable addition to modern animal farming process. However, because of their inconsistent effects and inadequate knowledge of the mechanisms of action, their usage as a feed additive has been limited. This review offers a comprehensive assessment of the applications of PFAs as an effective feed supplement in swine and poultry nutrition. In summary, this comprehensive review provides current knowledge, identifies gaps in research and emphasizes the potential of phytogenic additives to foster sustainable and healthier livestock production systems while addressing the global concerns associated with antibiotic use in livestock farming. [ABSTRACT FROM AUTHOR]

The effects of heat stress (HS) caused by high temperatures continue to be a global concern in poultry production. Poultry birds are homoeothermic, however, modern‐day chickens are highly susceptible to HS due to their inefficiency in dissipating heat from their body due to the lack of sweat glands. During HS, the heat load is higher than the chickens' ability to regulate it. This can disturb normal physiological functioning, affect metabolism and cause behavioural changes, respiratory alkalosis and immune dysregulation in birds. These adverse effects cause gut dysbiosis and, therefore, reduce nutrient absorption and energy metabolism. This consequently reduces production performances and causes economic losses. Several strategies have been explored to combat the effects of HS. These include environmentally controlled houses, provision of clean cold water, low stocking density, supplementation of appropriate feed additives, dual and restricted feeding regimes, early heat conditioning and genetic selection of poultry lines to produce heat‐resistant birds. Despite all these efforts, HS still remains a challenge in the poultry sector. Therefore, there is a need to explore effective strategies to address this long‐lasting problem. The most recent strategy to ameliorate HS in poultry is early perinatal programming using the in ovo technology. Such an approach seems particularly justified in broilers because chick embryo development (21 days) equals half of the chickens' posthatch lifespan (42 days). As such, this strategy is expected to be more efficient and cost‐effective to mitigate the effects of HS on poultry and improve the performance and health of birds. Therefore, this review discusses the impact of HS on poultry, the advantages and limitations of the different strategies. Finally recommend a promising strategy that could be efficient in ameliorating the adverse effects of HS in poultry. [ABSTRACT FROM AUTHOR]

Following a request from the European Commission, EFSA was asked to deliver a scientific opinion on the safety and efficacy of Saccharomyces cerevisiae DSM 34246 as a zootechnical feed additive for dogs and cats. The additive, with the trade name Canobios‐BL, is intended for use in feed for cats and dogs at a proposed minimum inclusion level of 5 × 109 CFU/kg complete feed. Saccharomyces cerevisiae is considered by EFSA to be suitable for the qualified presumption of safety approach to safety assessment. Since the identity of the active agent has been clearly established and the additive is composed by dried cells of the active agent and an emulsifier, that are not expected to introduce any risk, the additive is considered safe for the target species. Canobios‐BL is not a skin or eye irritant but should be considered a skin and respiratory sensitiser. Canobios‐BL is considered to be efficacious in feedingstuffs for dogs and cats at the use level 5 × 109 CFU/kg complete feed. [ABSTRACT FROM AUTHOR]

Following a request from the European Commission, EFSA was asked to deliver a scientific opinion on the safety and efficacy of the coccidiostat salinomycin sodium (Sacox®) for rabbits for fattening. The EFSA Panel on Additives and Products or Substances used in Animal Feed (FEEDAP) concluded that the use of salinomycin sodium (SAL‐Na) from Sacox® does not raise safety concerns for the target species, consumers, users and the environment with regard to the production strain. In the absence of adequate tolerance studies, the FEEDAP Panel could not conclude on the safety of SAL‐Na from Sacox® for rabbits for fattening. The FEEDAP Panel concluded that the additive is safe for the consumer when it is used at the proposed maximum level of 25 mg SAL‐Na/kg complete feed for rabbits and a withdrawal period of 1 day is respected. The following maximum residue limits (MRL) are proposed for the marker residue compound salinomycin (SAL): 0.2 and 0.03 mg SAL/kg for liver and kidney, respectively. The additive is not irritant to skin and eyes but should be considered a potential dermal and respiratory sensitiser. A risk for inhalation toxicity could not be excluded. The use of the SAL‐Na from Sacox® in feed for rabbits for fattening up to the highest proposed level will not pose a risk for the terrestrial and aquatic compartment and ground water. The risk of secondary poisoning can be excluded for worm‐eating birds and mammals, while it cannot be excluded for fish‐eating birds and mammals. The FEEDAP Panel concludes that SAL‐Na from Sacox® at the minimum concentration of 20 mg SAL‐Na/kg complete feed has the potential to control coccidiosis in rabbits for fattening. Development of resistance to SAL‐Na of field Eimeria spp. strains isolated from rabbits for fattening should be monitored. [ABSTRACT FROM AUTHOR]

Following a request from the European Commission, the Panel on Additives and Products or Substances used in Animal Feed (FEEDAP) was asked to deliver a scientific opinion on the efficacy of ROVABIO® ADVANCE (liquid and solid) which contains endo‐1,4‐beta‐xylanase and endo‐1,3(4)‐beta‐glucanase produced with Talaromyces versatilis IMI 378536 and DSM 26702 as a zootechnical feed additive for weaned piglets at the recommended use level of 1800 U xylanase and 1250 U glucanase per kg feed. In a previous assessment, three long‐term trials in weaned piglets were submitted. Two of them were considered to support the efficacy of the additive while a third trial was not further considered due to the large number of veterinary treatments applied. A new trial was provided to support the efficacy of the additive, but it did not show a significant improvement of the performance parameters at the minimum recommended use level. Due to the lack of sufficient data, the FEEDAP Panel is not in the position to conclude on the efficacy of the additive for the target species. [ABSTRACT FROM AUTHOR]

Following a request from the European Commission, EFSA was asked to deliver a scientific opinion on the safety and efficacy of an essential oil obtained from the wood of Juniperus deppeana Steud. (cedarwood Texas oil), when used as a sensory additive for all animal species. The EFSA Panel on Additives and Products or Substances used in Animal Feed (FEEDAP) concluded that the essential oil under assessment is safe up to the maximum proposed use levels in complete feed of 15 mg/kg for veal calves (milk replacer), cattle for fattening, sheep, goats, horses, dogs, salmonids and ornamental fish. For the other species, the calculated safe concentrations in complete feed were 5 mg/kg for chickens for fattening, 8 mg/kg for laying hens, 7 mg/kg for turkeys for fattening, 10 mg/kg for piglets, 12 mg/kg for pigs for fattening, 14 mg/kg for sows and dairy cows, 8.5 mg/kg for rabbits and 4 mg/kg for cats. These conclusions were extrapolated to other physiologically related species. For any other species, the additive was considered safe at 4 mg/kg complete feed. The use of cedarwood Texas oil in water for drinking was considered safe provided that the total daily intake of the additive does not exceed the daily amount that is considered safe when consumed via feed. No concerns for consumers and the environment were identified following the use of the additive up to the maximum proposed use level in feed. The additive under assessment should be considered as irritant to skin and eyes, and as a skin and respiratory sensitiser. Since the individual components of cedarwood Texas oil are recognised to flavour food and their function in feed would be essentially the same as that in food, no further demonstration of efficacy was considered necessary. [ABSTRACT FROM AUTHOR]

Following a request from the European Commission, EFSA was asked to deliver a scientific opinion on the safety and efficacy of tinctures obtained from the dried leaves of Ginkgo biloba L. (ginkgo tinctures) when used as sensory additives. The tinctures are water/ethanol solutions with a dry matter content of 5.7% (tincture A) and 3.0% (tincture B). The EFSA Panel on Additives and Products or Substances used in Animal Feed (FEEDAP) concluded that the additives under assessment are safe for the target species at the following concentrations in complete feed: (i) ginkgo tincture A at 240 mg/kg for horses and 750 mg/kg for dogs; (ii) ginkgo tincture B at 600 mg/kg for horses and 50 mg/kg for all other animal species. No safety concern would arise for the consumer from the use of ginkgo tinctures up to the maximum proposed use level in feed for the target species. The tinctures should be considered as irritants to skin and eyes, and as dermal and respiratory sensitisers. The use of ginkgo tinctures at the proposed use levels in feed for the target species is not considered to be a risk to the environment. While the available data indicate that Ginkgo preparations have a distinctive flavour profile, there is no evidence that ginkgo tinctures would impart flavour to a food or feed matrix. Therefore, the FEEDAP Panel cannot conclude on the efficacy of the additives. [ABSTRACT FROM AUTHOR]

Following a request from the European Commission, EFSA was asked to deliver a scientific opinion on the safety of the feed additive consisting of endo‐1,4‐β‐xylanase (produced with Trichoderma reesei CBS 143953), subtilisin (produced with Bacillus subtilis CBS 143946) and α‐amylase (produced with Bacillus amyloliquefaciens CBS 143954) (Avizyme® 1505) as a zootechnical feed additive for all poultry species. The additive is authorised in feed for chickens and turkeys for fattening, ducks and laying hens. In 2020, the FEEDAP Panel issued an opinion for the renewal of the authorisation of the additive for the species/categories for which there is an authorisation, a reduction of the minimum recommended level in turkeys for fattening and the extension of use to all poultry species. In that assessment, the Panel could not conclude on the safety of the additive due to uncertainties on the characterisation of the production strains and the possible presence of their viable cells and DNA in the final product. Moreover, limitations were identified in the xylanase specifications and xylanase method of analysis. The applicant submitted information to address the limitations previously identified. The Panel concluded that the additive is safe for the target species under the proposed conditions of use. The use of Avizyme® 1505 in animal nutrition is considered safe for the consumer and the environment. The additive is a mild irritant to skin and eyes; it is not a dermal sensitiser but should be considered a respiratory sensitiser. The additive is efficacious in ducks at 75 U endo‐1,4‐β‐xylanase, 1000 U subtilisin and 100 U α‐amylase/kg of complete feed. In other poultry species for fattening (including turkeys), reared for breeding and reared for laying, the additive is efficacious at 187.5 U endo‐1,4‐β‐xylanase, 2500 U subtilisin and 250 U α‐amylase per kg of complete feed and at 300 U endo‐1,4‐β‐xylanase, 4000 U subtilisin and 400 U α‐amylase per kg of complete feed for all poultry species for laying (except for ducks). [ABSTRACT FROM AUTHOR]

Following a request from the European Commission, EFSA was asked to deliver a scientific opinion on the safety and efficacy of a tincture from the leaves of Eucalyptus globulus Labill. (eucalyptus tincture) when used as a sensory additive for all animal species. The product is a ■■■■■ solution, with a dry matter content of ~ 1.86%, which contains on average 0.454% phenolic acids and flavonoids (of which 0.280% was gallic acid), 0.0030% 1,8‐cineole and 0.00012% methyleugenol. In the absence of analytical data on the occurrence of mono‐ or diformylated adducts of acylphloroglucinols with terpenes in the tincture and in the absence of toxicity data, the Panel on Additives and Products or Substances used in Animal Feed (FEEDAP) could not conclude on the use of eucalyptus tincture for long‐living and reproductive animals. For short‐living animals (species for fattening), the additive was considered of no concern at 4 mg/kg complete feed for chickens for fattening, 5 mg/kg for turkeys for fattening, 6 mg/kg for piglets and rabbits for meat production, 7 mg/kg for pigs for fattening, 16 mg/kg for veal calves (milk replacer), 14 mg/kg for cattle for fattening, sheep/goats and horses for fattening, and 15 mg/kg for salmonids. These levels were extrapolated to physiologically related minor species. No safety concern would arise for the consumer from the use of eucalyptus tincture up to the levels in feed considered of no concern. Eucalyptus tincture should be considered as irritant to skin and eyes, and as a dermal and respiratory sensitiser. The use of eucalyptus tincture as a flavour in animal feed was not expected to pose a risk for the environment. Since the leaves of E. globulus and their preparations were recognised to flavour food and their function in feed would be essentially the same, no demonstration of efficacy was considered necessary. [ABSTRACT FROM AUTHOR]

Following a request from the European Commission, EFSA was asked to deliver a scientific opinion on the assessment of the application for the renewal of the authorisation of l‐cystine as nutritional feed additive. The additive is authorised for use in all animal species (3c391). The applicant has provided evidence that the additive currently in the market complies with the existing conditions of authorisation. The EFSA Panel on Additives and Products or Substances used in Animal Feed concluded that the use of the feed additive in animal nutrition remains safe for the target species, the consumers and the environment. As regards the safety for the user, l‐cystine is not an irritant to skin or eyes and is not a skin sensitiser. Exposure by inhalation of persons handling the additive cannot be excluded. The present application for the renewal of the authorisation does not include any modification proposal that would have an impact on the efficacy of the additive and therefore there is no need for reassessing the efficacy. [ABSTRACT FROM AUTHOR]

Following a request from the European Commission, EFSA was asked to deliver a scientific opinion on the safety and efficacy of Macleaya cordata (Willd.) R. Br. extract and leaves (Sangrovit® Extra) as a zootechnical feed additive for suckling and weaned piglets and other growing Suidae. The additive is standardised to contain a concentration of the sum of the four alkaloids sanguinarine, chelerythrine, protopine and allocryptopine of 1.25%, with 0.5% sanguinarine. Owing to the presence of the DNA intercalators sanguinarine and chelerythrine, a concern for genotoxicity was identified. The EFSA Panel on Additives and Products or Substances used in Animal Feed (FEEDAP) had no safety concerns for the target species when the additive is used at the recommended level of 0.750 mg sanguinarine/kg complete feed for suckling and weaned piglets and other growing Suidae. Since in all consumer categories the exposure to sanguinarine and chelerythrine via the use of Sangrovit® Extra exceeds the threshold of toxicological concern of 0.0025 μg/kg bw per day for DNA reactive mutagens and/or carcinogens, the FEEDAP Panel could not conclude on the safety for the consumers. The additive was shown to be irritant to the eyes but not irritant to skin or a skin sensitiser. The FEEDAP Panel could not exclude the potential of the additive to be a respiratory sensitiser. When handling the additive, exposure of unprotected users to sanguinarine and chelerythrine may occur. Therefore, to reduce the risk, the exposure of users should be reduced. The use of Sangrovit® Extra as a feed additive under the proposed conditions of use was considered safe for the environment. The additive Sangrovit® Extra had the potential to be efficacious in improving performance of weaned piglets at 0.600 mg sanguinarine/kg complete feed. This conclusion was extended to suckling piglets and extrapolated to other growing Suidae. [ABSTRACT FROM AUTHOR]

Following a request from the European Commission, EFSA was asked to deliver a scientific opinion on the assessment of the application of renewal of Limosilactobacillus fermentum NCIMB 30169 as a technological feed additive (functional group: silage additives) for all animal species. The applicant has provided evidence that the additive currently on the market complies with the existing terms of the authorisation. The EFSA Panel on Additives and Products or Substances used in Animal Feed (FEEDAP) concluded that the additive remains safe for all animal species, consumers, and the environment. Regarding user safety, the additive should be considered a skin and respiratory sensitiser. No conclusions can be drawn on the eye irritancy potential of the additive. There is no need for assessing the efficacy of the additive in the context of the renewal of the authorisation. [ABSTRACT FROM AUTHOR]

Following a request from the European Commission, EFSA was asked to deliver a scientific opinion on the assessment of the application for renewal of authorisation of Lacticaseibacillus paracasei ATCC PTA‐6135 as a technological additive (functional group: silage additive) for all animal species. The applicant has provided evidence that the additive currently on the market complies with the existing terms of the of authorisation. The EFSA Panel on Additives and Products or Substances used in Animal Feed (FEEDAP) concluded that the active agent L. paracasei ATCC PTA‐6135 remains safe for all animal species, consumers and the environment. Regarding user safety, the panel concluded that owing to the nature of the additive, L. paracasei ATCC PTA‐6135 should be considered a potential skin and respiratory sensitiser, and any exposure through the skin and respiratory tract is considered a risk. In the absence of data, no conclusion could be drawn on the eye irritation potential of the additive. There is no need for assessing the efficacy of the additive in the context of the renewal of the authorisation. [ABSTRACT FROM AUTHOR]

Following a request from the European Commission, EFSA was asked to deliver a scientific opinion on the safety and efficacy of a tincture from the dried flower bud of Syzygium aromaticum (L.) Merr. & L.M. Perry (clove tincture) when used as a sensory additive in feed and water for drinking for all animal species. The product is a ■■■■■) solution, with a dry matter content of ~ 1.66%. The product contains on average 0.511% phenolic acids (of which 0.0344% were flavonoids), 0.039% eugenol, 0.00019% methyleugenol and 0.00008% estragole. The Panel on Additives and Products or Substances used in Animal Feed (FEEDAP) concluded that the use of clove tincture is very unlikely to be of safety concern for the target species up to the maximum proposed use level of 50 mg clove tincture/kg complete feed for all animal species, except for horses, for which the proposed use level is 200 mg/kg complete feed. The FEEDAP Panel considers that the use in water for drinking alone or in combination with use in feed should not exceed the daily amount that is considered very unlikely to be of safety concern when consumed via feed alone. No safety concern would arise for the consumer and the environment from the use of clove tincture up to the maximum proposed use levels in feed. The additive under assessment should be considered as irritant to skin and eyes, and as a dermal and respiratory sensitiser. When handling the additive, exposure of unprotected users to methyleugenol and estragole may occur. Therefore, to reduce the risk, the exposure of the users should be minimised. Since the flower buds of S. aromaticum and their preparations were recognised to flavour food and their function in feed would be essentially the same, no demonstration of efficacy was considered necessary. [ABSTRACT FROM AUTHOR]

Following a request from the European Commission, EFSA was asked to deliver a scientific opinion on the safety and efficacy of citronella oil obtained from the leaves of Cymbopogon nardus (L.) Rendle, when used as a sensory additive for all animal species. The EFSA Panel on Additives and Products or Substances used in Animal Feed (FEEDAP) concluded that citronella oil from C. nardus is of low concern for long‐living and reproductive animals at the use levels in complete feed of 3.5 mg/kg for laying hens and rabbits, 6 mg/kg for sows and dairy cows, 9.5 mg/kg for sheep/goats and horses, 2.0 mg/kg for cats and 10 mg/kg for dogs. For short‐living animals (species for fattening), the additive was considered of no concern at concentrations of 18 mg/kg in chickens for fattening, 24 mg/kg in turkeys for fattening, 20 mg/kg for piglets, pigs for fattening, veal calves (milk replacer), cattle for fattening, sheep/goats for meat production, horses for meat production and rabbits for meat production, and 30 mg/kg for salmonids. The conclusions were extrapolated to physiologically related minor species. For any other species, the additive is considered of low concern at 2.0 mg/kg complete feed. The use of citronella oil in animal feed is expected to be of no concern for the consumers and for the environment. The essential oil under assessment should be considered as irritant to skin and eyes and as a dermal sensitiser. When handling the essential oil, exposure of unprotected users to methyleugenol may occur. Therefore, to reduce the risk, the exposure of the users should be minimised. Since the leaves of C. nardus and its preparations were recognised to flavour food and its function in feed would be essentially the same as that in food, no further demonstration of efficacy was considered necessary. [ABSTRACT FROM AUTHOR]

Following a request from the European Commission, EFSA was asked to deliver a scientific opinion on the safety and efficacy of muramidase produced with Trichoderma reesei DSM 32338 (Balancius™) as a feed additive for laying hens. The additive is already authorised as a zootechnical additive (functional group: other zootechnical additives) for chickens, turkeys and minor poultry species for fattening or reared for breeding, and for weaned piglets. The enzyme is produced by fermentation with a genetically modified strain of Trichoderma reesei; viable cells of the production strain and its recombinant DNA were not detected in the additive. The EFSA Panel on Additives and Products or Substances used in Animal Feed (FEEDAP) concluded that the additive does not give rise to safety concerns regarding the genetic modification of the production strain. Based on the data available from a sub‐chronic oral toxicity study, the Panel concluded that the additive is safe for laying hens at the maximum recommended level of 60,000 LSU(F) (muramidase activity units)/kg feed. The Panel also concluded that the additive is safe for the consumers and the environment. The liquid formulation of the additive is considered not irritant to the skin or eyes. The solid formulation of the additive is considered not irritant to the skin. The Panel cannot conclude on the potential of the additive (both formulations) to be a dermal sensitiser or on the potential of the solid formulation to be irritant to the eyes. Due to the proteinaceous nature, both forms of the additive should be considered respiratory sensitisers. The additive has the potential to be efficacious as a zootechnical additive for laying hens at 30,000 LSU(F)/kg feed. [ABSTRACT FROM AUTHOR]

Following a request from the European Commission, the Panel on Additives and Products or Substances used in Animal Feed (FEEDAP) was asked to deliver a scientific opinion on the efficacy of ferrous lysinate sulfate (Plexomin® L‐Fe) for all animal species. The additive has not been previously authorised as a feed additive in the European Union (EU). The safety of the additive for the target species, consumer, user and the environment has already been assessed in previous opinions. However, the efficacy remained inconclusive due to the absence of evidence of the bioavailability of the iron contained in the additive in the trials submitted either with chickens for fattening or with weaned piglets. For the present assessment, the applicant submitted a recalculation of the previous data on weaned piglets, which did not show evidence of bioavailability. Therefore, in the absence of adequate data, the FEEDAP Panel could not conclude on the efficacy of ferrous lysinate sulfate for all animal species. [ABSTRACT FROM AUTHOR]

Following a request from the European Commission, EFSA was asked to deliver a scientific opinion on the assessment of the application for renewal of Lentilactobacillus buchneri ATCC PTA‐2494 as a technological additive (functional group: silage additives) for all animal species. The applicant has provided evidence that the additive currently on the market complies with the existing terms of the authorisation. The EFSA Panel on Additives and Products or Substances used in Animal Feed (FEEDAP) concluded that the additive remains safe for all animal species, consumers and the environment. Regarding user safety, the Panel considers that any exposure through skin and respiratory tract is considered a risk. The Panel cannot conclude on the eye irritation potential of the additive due to the lack of data. There is no need for assessing the efficacy of the additive in the context of the renewal of the authorisation. [ABSTRACT FROM AUTHOR]

Following a request from the European Commission, EFSA was asked to deliver a scientific opinion on the assessment of the application for renewal of Lentilactobacillus buchneri ATCC PTA‐6138 as a technological additive (functional group: silage additives) for all animal species. The applicant has provided evidence that the additive currently on the market complies with the existing terms of the authorisation. The EFSA Panel on Additives and Products or Substances used in Animal Feed (FEEDAP) concluded that the additive remains safe for all animal species, consumers and the environment. Regarding user safety, the additive should be considered as a skin and respiratory sensitiser. No conclusions could be drawn on the eye irritancy potential of the additive. There is no need for assessing the efficacy of the additive in the context of the renewal of the authorisation. [ABSTRACT FROM AUTHOR]

Following a request from the European Commission, EFSA was asked to deliver a scientific opinion on the assessment of the application for renewal of Lactiplantibacillus plantarum (formerly Lactobacillus plantarum) DSM 18112 as a technological additive (functional group: silage additive) for all animal species. The applicant has provided evidence that the additive currently on the market complies with the existing conditions of authorisation. The EFSA Panel on Additives and Products or Substances used in Animal Feed (FEEDAP) concluded that the additive remains safe for all animal species, consumers and the environment. Regarding user safety, the Panel concluded that owing to the nature of the additive, Lactiplantibacillus plantarum DSM 18112 should be considered a potential skin and respiratory sensitiser, and that any exposure through the skin and respiratory tract is considered a risk. The Panel could not conclude on the eye irritation potential of the additive due to the lack of data. There is no need for assessing the efficacy of the additive in the context of the renewal of the authorisation. [ABSTRACT FROM AUTHOR]

Following a request from the European Commission, EFSA was asked to deliver a scientific opinion on the assessment of the application for renewal of Lactiplantibacillus plantarum (formerly Lactobacillus plantarum) ATCC 55943 as a technological additive (functional group: silage additive) for all animal species. The applicant has provided evidence that the additive currently on the market complies with the existing conditions of authorisation. The EFSA Panel on Additives and Products or Substances used in Animal Feed (FEEDAP) concluded that the additive remains safe for all animal species, consumers and the environment. Regarding user safety, the Panel concluded that owing to the nature of the additive, Lactiplantibacillus plantarum ATCC 55943 should be considered a potential skin and respiratory sensitiser, and any exposure through the skin and respiratory tract is considered a risk. The Panel could not conclude on the eye irritation potential of the additive due to the lack of data. There is no need for assessing the efficacy of the additive in the context of the renewal of the authorisation. [ABSTRACT FROM AUTHOR]

Following a request from the European Commission, EFSA was asked to deliver a scientific opinion on the safety and efficacy of 6‐phytase (Quantum® Blue) as a zootechnical feed additive for fin fish. The additive is authorised for use in poultry and pigs. The additive is available in solid and liquid forms, and the 6‐phytase contained in the product is produced by fermentation with a genetically modified strain of Trichoderma reesei. The Panel on Additives and Products or Substances used in Animal Feed (FEEDAP) concluded that the genetic modification of the production strain does not give rise to safety concerns; viable cells of the production strain and its DNA were not detected in the final products. The FEEDAP Panel concluded that, based on the data available, the additive tested is safe for fin fish at the highest recommended level of 2500 phytase activity unit (FTU)/kg complete feed. The Panel concluded that Quantum® Blue is not an irritant to skin and eyes nor a skin sensitiser. Owing to the proteinaceous nature of the active substance, 6‐phytase (Quantum® Blue) is considered a respiratory sensitiser. The use of Quantum® Blue as a feed additive is considered safe for the environment. The additive is considered to be efficacious as a zootechnical additive for salmonids and ornamental fish at 500 FTU/kg complete feed and other fin fish at 2500 FTU/kg complete feed. [ABSTRACT FROM AUTHOR]

【Objective】The study aimed to explore the effects of Enterococcus faecium extracellular vesicles (EfmEV) on growth performance, immunity, intestinal morphology and fecal microbiota in mice, providing theoretical basis for the application of E. faecium as a microbial feed additive in animal production.【Method】A total of 20 male C57BL/6 mice were randomly divided into control group and experimental group with 10 replicates per group and 1 mouse per replicate. The experiment lasted for 5 days. Mice in the experimental group were given 500 μg Efm-EV suspension every other day by intragastric administration, with 150 μL for each mouse, and mice in the control group were given the same volume of PBS. Daily feed intake, and body weight at the beginning and end of the experiment of each mouse were recorded, average daily gain (ADG), average daily feed intake (ADFI) and feed/gain (F/G) of mice were calculated accordingly.【Result】Compared with the PBS treatment group, no significant change was observed in the final body weight (FBW), ADG, ADFI and F/G of mice in the Efm-EV treatment group. The serum immunoglobulin A (IgA) and immunoglobulin G (IgG) levels in the Efm-EV group were significantly decreased compared with those in PBS treatment group (P ＜ 0.05), while the serum interleukin-10 (IL10) level was significantly increased (P ＜ 0.05), and there was no significant difference in serum tumor necrosis factor-α (TNF-α) level. Compared with the PBS treatment, Efm-EV treatment significantly increased duodenal villus height/crypt depth ratio (VH/CD) (P ＜ 0.05). There were no significant changes in villus height (VH), crypt depth (CD) of duodenum, and VH, CD and VH/CD of jejunum and ileum. Efm-EV intragastric administration increased the number of Firmicutes and decreased the number of Bacteroidetes at the phylum level, increased the abundance of Lachnospiraceae and decreased the abundance of Muribaculaceae at the family level, significantly increased the abundance of Ruminococcaceae UCG009 at the genus level (P＜ 0.05).【Conclusion】Intragastric administration of Efm-EV can improve the anti-inflammatory level, the duodenal morphology and the relative abundance of Ruminococcaceae UCG009 in mice. [ABSTRACT FROM AUTHOR]

Introduction: Obesity is a multifactorial disease and is closely related to changes in eating behavior. In theory, the body has a complex homeostatic mechanism for the regulation of hunger, satiety, and adiposity, but in individuals with obesity this system is dysfunctional. In addition, food has been the target of pleasure, especially foods with high energy density, rich in sugars, fats and/or chemical additives, called ultra-processed, which have high palatability, motivating hedonic behavior and consequently reward systems. Objective: To describe the mechanisms involved in the regulation of hunger and satiety, as well as the motivations to consume certain foods and amounts, motivated by hedonic control, and their associations with the eating behavior of individuals with obesity. Materials and Methods: This is a narrative review of the literature, which was carried out through the Pubmed, Google Scholar and Cochrane databases, with priority being included articles published after the year 2018 in English and Portuguese. Results: Studies have shown a strong association between the consumption of ultra-processed foods and obesity due to their high energy density and palatability, being targeted in hedonic eating behaviors due to the activation of reward systems. In addition, obesity is related to disturbances in homeostatic controls, such as insulin and leptin resistance, so the control of hunger, satiety and adiposity become dysfunctional, which makes treatment even more difficult. Conclusion: The complexity surrounding obesity and its treatment shows the need for more public policies and studies to better understand the mechanisms involved. [ABSTRACT FROM AUTHOR]

EFSA collected data on co‐formulants contained in plant protection product (PPP) formulations for representative uses as part of applications for approval or renewal of active substances, to collate the information available during the peer review process. Information was extracted from pesticide active substances dossiers for which a peer review output was issued between January 2019 and March 2022, in which a total of 182 co‐formulants were found. Most of the data submitted on the identity and physicochemical properties of the co‐formulants are complete as required by Regulation (EU) 284/2013. The data related to (eco)toxicological properties mainly refers to the safety data sheet of the individual co‐formulants and, if available, to registration dossiers under Regulation (EC) 1907/2006 (REACH). Fifty‐three % of the identified co‐formulants have been registered under REACH and about 80% of the 53% are registered for more than 1000 tonnes a year for which registrants are required to submit the most complete data set under REACH. For the remaining percentage, a more limited range of toxicological information is available: ECHA investigated whether they are exempted from registration under REACH. Co‐formulants contained in plant protection products may also be subject to other European legislation such as the regulations on biocidal active substances, food/feed additives, food contact materials, or are included in lists of excipients or cosmetic ingredients. On 28 September 2023, the European Commission requested EFSA to inform the European Commission if and which of the co‐formulants listed in this EFSA technical report, fulfils any of the criteria 1 to 9 in the Annex to Commission Implementing Regulation (EU) No 574/2023 and is not already listed in Annex III to Regulation (EC) No 1107/2009. Out of the 182 co‐formulants, 8 meet at least one of the 9 criteria and are concluded as unacceptable. For twenty‐two co‐formulants no CAS/EC numbers were available in Volume 4 of the respective DAR/RAR. Observations highlighted in the present report can serve Member State risk assessors and risk managers to harmonise the assessment of co‐formulants in collaboration with the EU agencies (EFSA, ECHA). This publication is linked to the following EFSA Supporting Publications article: http://onlinelibrary.wiley.com/doi/10.2903/sp.efsa.2022.EN-7547/full [ABSTRACT FROM AUTHOR]

This study was conducted to evaluate the effect of feeding three levels of concentrate 2%, 3%, and 4% with or without additives of N-carbamylglutamate (NCG) on the digestibility, feed efficiency, and daily gain of Shami goats. Twenty-four male goats were used aged 8-10 months with an initial weight 32.687 ± 1.52kg divided into six treatments in a 2×3 factorial experiment/ Completely Randomized Design. Individual feeding was used for 70 days preceded by 14 days as an adaptation period. The results of increasing concentrate showed a non-significant increase in nutrients intake with superiority of NCG treatments and a significant increase (P<0.01) of ether extract intake. Non-significant increase in digestibility with increasing feed intake. Significant increases (P<0.01) in total and daily gain of 3% concentrate with or without NCG and non-significant increases for final weight. The better feed efficiency with 3% concentrate then 2% and 4% respectively. In conclusion: A level of feeding 3% had a positive effect on animal performance and the best for fattening. [ABSTRACT FROM AUTHOR]

Background: Eimeriosis in calves, often treated with harsh chemicals, leads to significant health and economic burdens. This study explores natural coniferous-phytogenic feed additives as an innovative solution, focusing on their efficacy against Eimeria bovis and Eimeria ellipsoidalis, two common eimeriosis pathogens in calves. Methods: The authors conducted experiments to analyze the therapeutic and preventive effectiveness of the use of a coniferous-phytogenic immunomodulator in the diet of calves from 5-7 days of age and a coniferous and salicylic acid feed additive from 28-30 days of age in case of eimeriosis infestation at a dose of 5.0, 10.0, and 15.0 ml, once a day, in courses of 2 weeks without intervals with a small amount of warm water. Results: The extensivity of the coniferous and salicylic acid feed additive against the eimeriosis infestation in calves caused by E. bovis showed maximum result. Both preparations were willingly consumed by calves and eliminated the signs of diarrheal dyspeptic syndrome and gastroenteritis. The inclusion of coniferousphytogenic feed additives in the calves' diet from 5-7 days of age once a day, in courses of 2 weeks without intervals, contributed to 100% effectiveness against E. bovis and E. ellipsoidalis. Conclusion: The study concludes that coniferous-phytogenic feed additives are highly effective in treating and preventing eimeriosis in calves, showing 100% effectiveness against the targeted pathogens. These additives also contribute to the overall health and well-being of the animals by alleviating related symptoms. [ABSTRACT FROM AUTHOR]

The emerging trend of employing 4 or more axes multi-purpose and gantry industrial robots in large format additive manufacturing presents numerous opportunities as well as challenges. The capacity to handle substantial material quantities and rapidly produce prototypes, instrumentation, and final products of considerable dimensions necessitates the formulation of a well-suited production strategy. This involves setting production parameters to minimize material consumption and production time, considering the limitations of the utilized technologies, and ensuring the final product's quality. While slicers are commonly employed for establishing manufacturing strategies and production parameters, most additive manufacturing slicers are optimized for planar 3 axes 3D printing. This limitation hinders their ability to generate non-planar and freeform toolpaths. To overcome this constraint, this paper delves into the utilization of parametric modelling as a potent tool in the realm of non-planar additive manufacturing. It explores the possibilities offered by Rhinoceros Grasshopper software in designing toolpath strategies and fabricating non-planar layers. The paper addresses the associated challenges and limitations of parametric modelling, including computational complexity and the requirement for specialized software and expertise. It emphasizes the crucial need to strike a balance between design complexity and manufacturability to ensure the successful implementation of non-planar additive manufacturing processes. [ABSTRACT FROM AUTHOR]

The burgeoning population of Indonesia has led to a heightened demand for animal protein, particularly within the broiler industry. However, the commercial broiler feed tends to be costly due to its reliance on imported ingredients. Therefore, the potential utilization of Nile tilapia gills as an additional protein source for broiler feed has been explored in this study. The primary objective was to investigate how tilapia gill powder affects the histological structure of the small intestine, pectoralis muscle, and overall growth performance of broilers. For the study, a total of 200 dayold male Cobb-500 broiler chicks were utilized, employing a completely randomized design with five treatments and five replications. Each replication consisted of 8-day-old chicks. Tilapia gill powder was incorporated into the basal feed at varying concentrations: Control (basal feed), tilapia gill powder 1 (0.25% tilapia gill powder/kg basal feed), tilapia gill powder 2 (0.5% tilapia gill powder/kg basal feed), tilapia gill powder 3 (1% tilapia gill powder/kg basal feed), and tilapia gill powder 4 (2% tilapia gill powder/kg basal feed). Comprehensive histological analysis and growth performance assessments were carried out. The outcomes revealed that the tilapia gill powder 4 group, administered at an optimal concentration of 2% tilapia gill powder/kg basal feed, exhibited significant enhancements in small intestine morphology, pectoralis muscle structure, and overall chicken growth performance when compared to the control group. The supplementation of tilapia gill powder at this optimal concentration (2% tilapia gill powder/kg basal feed) showcased a favorable impact on broiler performance. [ABSTRACT FROM AUTHOR]

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The continuous use of growth promoter (AGP) antibiotics in rearing chickens will cause residues in the meat. Finding a natural antibiotic derived from herbal plants that noni fruit is necessary. Microencapsulation of noni fruit extract (MNFE) was reported to positively affect poultry nutrition absorption. The research was conducted to determine the effect of MNFE with maltodextrin and its implementation as a feed additive to nutrient digestibility and the performance of Sentul chicken. The experiment used 100 Sentul chicks with a Completely Randomized Design (CRD), with five treatments and four replications. The treatment consisted of basal ration T0 (control), T1 (basal ration + 50 mg/kg Zinc-Bacitracin), T2 (basal ration + 125 mg/kg MNFE), T3 (basal ration + 125 mg/kg MNFE), and T4 (basal ration + 375 mg/kg MNFE). Variables observed were nutrient digestibility (crude protein digestibility, dry matter digestibility, organic matter digestibility, metabolic energy, nitrogen retention) and performance (feed consumption, body weight gain, feed efficiency). The results showed that Crude Protein Digestibility (CPD), Dry Matter Digestibility (DMD), Organic Matter Digestibility (OMD), metabolic energy, nitrogen retention, and performance of Sentul chicken were significantly (p<0.05) different in the group when compared to controls. It was concluded that adding 250 mg/kg MNFE could improve Sentul chicken's best nutrient digestibility and growth performance. It is recommended to be used as a feed additive to replace AGP. [ABSTRACT FROM AUTHOR]

Considering the serious risks posed by the heavy metals present in pig diets, monitoring and controlling the amount of these metals is crucial. The laser-induced breakdown spectroscopy (LIBS) is a promising technique for performing elemental analysis due to its unique advantages, such as rapid, in situ, nondestructive, and online detection of various minerals. In order to improve the detection accuracy and detection limit (LOD) of elements, the spatial constraint combined with laser-induced breakdown spectroscopy (CC-LIBS) is used to detect the quantity of zinc in pig feed and achieve the detection of samples with lower concentrations. Zn I 480.86 nm is selected as the characteristic spectral line, and the effects of different two-dimensional (cylindrical) and three-dimensional (hemispherical, truncated cone) cavities on the enhancement factor are compared under different time delays. The results show that the optimal conditions include a truncated cone cavity D5H3, a delay time of 2.08, and an enhancement factor of 3.01 associated with the analytical spectral line. The detection limit of zinc in samples under CC-LIBS is 62.67 mg/kg (the recommended quantity of zinc in pig diet mentioned in the safe use of feed additives is 43–80 mg/kg), which is 35.65% lower than that under the LIBS (97.39 mg/kg). Therefore, this work provides a new test basis and idea for the detection of zinc in pig feed. [ABSTRACT FROM AUTHOR]

l-Threonine is an important feed additive with the third largest market size among the amino acids produced by microbial fermentation. The GRAS (generally regarded as safe) industrial workhorse Corynebacterium glutamicum is an attractive chassis for l-threonine production. However, the present l-threonine production in C. glutamicum cannot meet the requirement of industrialization due to the relatively low production level of l-threonine and the accumulation of large amounts of by-products (such as l-lysine, l-isoleucine, and glycine). Herein, to enhance the l-threonine biosynthesis in C. glutamicum, releasing the aspartate kinase (LysC) and homoserine dehydrogenase (Hom) from feedback inhibition by l-lysine and l-threonine, respectively, and overexpressing four flux-control genes were performed. Next, to reduce the formation of by-products l-lysine and l-isoleucine without the cause of an auxotrophic phenotype, the feedback regulation of dihydrodipicolinate synthase (DapA) and threonine dehydratase (IlvA) was strengthened by replacing the native enzymes with heterologous analogues with more sensitive feedback inhibition by l-lysine and l-isoleucine, respectively. The resulting strain maintained the capability of synthesizing enough amounts of l-lysine and l-isoleucine for cell biomass formation but exhibited almost no extracellular accumulation of these two amino acids. To further enhance l-threonine production and reduce the by-product glycine, l-threonine exporter and homoserine kinase were overexpressed. Finally, the rationally engineered non-auxotrophic strain ZcglT9 produced 67.63 g/L (17.2% higher) l-threonine with a productivity of 1.20 g/L/h (108.0% higher) in fed-batch fermentation, along with significantly reduced by-product accumulation, representing the record for l-threonine production in C. glutamicum. In this study, we developed a strategy of reconstructing the feedback regulation of amino acid metabolism and successfully applied this strategy to de novo construct a non-auxotrophic l-threonine producing C. glutamicum. The main end by-products including l-lysine, l-isoleucine, and glycine were almost eliminated in fed-batch fermentation of the engineered C. glutamicum strain. This strategy can also be used for engineering producing strains for other amino acids and derivatives. [ABSTRACT FROM AUTHOR]

Bacillus subtilis is one of the widely used probiotics, which has strong stability and can survive and reproduce in animal intestines. Bacillus subtilis has synergistic effects with other probiotics or prebiotics, which is widely used in microbial feed additives. Bacillus subtilis has more advantages in viability in gastrointestinal tract than lactic acid bacteria and Bifidobacterium. Bacillus subtilis can regulate the intestinal flora of animals, improve growth performance, enhance immunity and intestinal health. Based on the physiological characteristics of Bacillus subtilis, the paper describes its application in animal production and its functions in regulating intestinal health, inhibiting oxidative stress and enhancing immunity, aiming at providing reference for the related application of Bacillus subtilis in animal production. [ABSTRACT FROM AUTHOR]

Simple Summary: Exogenous enzymes can improve domestic ruminants' productive performance by improving the amount of nutrients obtained from their rations, mainly from the fibrous portions. Dietary inclusion of exogenous enzymes can act synergistically with endogenous enzymes from the rumen during digestion and can modify the populations of ruminal communities and methane emissions. In the present study, dietary supplementation of a multi-enzyme (M-E) complex (Optimax E®) at 0, 0.2, 0.4, and 0.8% of dry matter (DM) increased nutrient digestibility, daily weight gain, and net energy, and improved feed conversion and the Firmicutes/Bacteroidetes ratio. Results confirm that including Optimax E® can be used to improve lambs' performance. The objective of this study was to evaluate the effects of increasing levels of the M-E complex (xylanase, glucanase, cellulase, and invertase) Optimax E® on the performance of growing lambs, their digestibility, and their rumen microbiota, and to estimate NEm, NEg, and ruminal methane levels. Forty lambs (Katahdin x Dorset; 22.91 ± 4.16 kg) were randomly assigned to dietary concentrations of ME (0, 0.2, 0.4, and 0.8% DM) and fed individually for 77 days. Increasing M-E improved feed conversion (p < 0.05) as well as NEm and NEg (p < 0.05), which were associated with increased in vivo DM and NDF digestion (linear and quadratic p < 0.01). Few microbial families showed abundancy changes (Erysipelotrichaceae, Christensenellaceae, Lentisphaerae, and Clostridial Family XIII); however, the dominant phylum Bacteroidetes was linearly reduced, while Firmicutes increased (p < 0.01), resulting in a greater Firmicutes-to-Bacteroidetes ratio. Total Entodinium showed a quadratic response (p < 0.10), increasing its abundancy as the enzyme dose was augmented. The daily emission intensity of methane (per kg of DMI or AGD) was reduced linearly (p < 0.01). In conclusion, adding the M-E complex Optimax E® to growing lambs' diets improves their productive performance by acting synergistically with the rumen microbiota, modifying the Firmicutes-to-Bacteroidetes ratio toward more efficient fermentation, and shows the potential to reduce the intensity of greenhouse gas emissions from lambs. [ABSTRACT FROM AUTHOR]

Simple Summary: Aquaculture is predicted to play a crucial role in providing nutritional and healthy food for people worldwide. Under the Blue Transformation concept, aquaculture should be sustainable and resilient, minimize environmental impacts, and improve biosecurity and disease control. The use of essential oils and extracts of various species of edible and medicinal plants, herbs, and spices is considered promising as options for aqua feed additives, paving a sustainable route of fish protection using safe, eco-friendly compounds in a more cost-effective way for treatment compared to antibiotics currently used. Our aim was the evaluation of the impact of Artemisia arborescens dietary supplementation on growth performance, biochemical and oxidative stress, and immunological parameters on gilthead seabream (Sparus aurata). Our findings suggested that the use of A. arborescens as a feed supplement has a compromised positive effect on the above-mentioned parameters of gilthead seabream; therefore, plant-origin feed additives should be used in fish species with caution. Fish infectious diseases are one of the main constraints of the aquaculture sector. The use of medicinal plants provides a sustainable way of protection using safe, eco-friendly compounds in a more cost-effective way of treatment, compared to antibiotics. The aim of the present study is the assessment of Artemisia arborescens (AA) feed-supplementation effects on gilthead seabream (Sparus aurata). Fish with an average initial body weight of 109.43 ± 3.81 g, were divided into two groups based on AA feed composition (A25 and A50). Following two months of ad libitum feeding, the effect of diets on fish weight and length were measured. Fish serum and mucus were analyzed for non-specific immune parameters (nitric oxide, lysozyme, myeloperoxidase, protease-/anti-protease activity, and complement), antibody responses, oxidative stress (cytochrome P450 1A1, metallothionein), and metabolism markers (total protein, alkaline phosphatase, and glucose). Expression levels of antioxidants (sod1, gpx1), cytokines (il-1b, il-10, tfgb1, and tnfa), hepcidin, and heat shock protein grp75 genes were measured in spleen samples. A results analysis indicated that A. arborescens use as a feed supplement has a compromised positive effect on the growth performance, immune response, and blood parameters of gilthead seabream. Overall, the suitability of A. arborescens as an efficient food supplement for gilthead seabream health improvement was investigated, setting the basis for its application assessment in Mediterranean aquaculture. [ABSTRACT FROM AUTHOR]