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886 results on '"Dubey, J.P."'

217. Cryptosporidium canis n. sp. from domestic dogs

Author

Fayer, R., Trout, J.M., Xiao, L., Morgan, U.M., Lal, A.A., Dubey, J.P., Fayer, R., Trout, J.M., Xiao, L., Morgan, U.M., Lal, A.A., and Dubey, J.P.

Abstract

Oocysts of Cryptosporidium, from the feces of a naturally infected dog and from an HIV-infected human, were identified as the previously reported canine genotype of Cryptosporidium parvum, hereafter referred to as Cryptosporidium canis n. sp. Also among the oocysts from the dog, a trace amount of C. parvum bovine genotype was detected. Cryptosporidium canis oocysts from both the dog and human were infectious for calves. Oocysts excreted by calf 1 (dog source) were approximately 90% C. canis and 10% C. parvum, whereas those excreted by calf 3 (human source) were 100% C. canis. Oocysts from calf 1 infected calf 2 resulting in excretion by calf 2 of oocysts 90% C. parvum and 10% C. canis. Oocysts of C. canis were not infectious for BALB/c neonatal mice or immunosuppressed C57 juvenile mice, although all control mice became infected with the C. parvum Beltsville isolate. Oocysts of C. canis from calf 1 and the human were structurally indistinguishable from oocysts of the C. parvum Beltsville isolate (bovine). However, C. canis oocysts differed markedly at the molecular level from all known species of Cryptosporidium based on sequence data for the 18S rDNA and the HSP 70 gene. The differences in genetics and host specificity clearly differentiate C. canis as a new species.

Published
2001

222. Neospora caninum antibodies in wild carnivores from Spain

Author

Sobrino, R., primary, Dubey, J.P., additional, Pabón, M., additional, Linarez, N., additional, Kwok, O.C., additional, Millán, J., additional, Arnal, M.C., additional, Luco, D.F., additional, López-Gatius, F., additional, Thulliez, P., additional, Gortázar, C., additional, and Almería, S., additional

Published
2008
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245. Biologic and genetic comparison of Toxoplasma gondii isolates in free-range chickens from the northern Pará state and the southern state Rio Grande do Sul, Brazil revealed highly diverse and distinct parasite populations

Author

Dubey, J.P., primary, Sundar, N., additional, Gennari, S.M., additional, Minervino, A.H.H., additional, Farias, N.A. da R., additional, Ruas, J.L., additional, dos Santos, T.R.B., additional, Cavalcante, G.T., additional, Kwok, O.C. H., additional, and Su, C., additional

Published
2007
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248. Seroprevalence of Neospora caninum in non-carnivorous wildlife from Spain

Author

Almería, S., primary, Vidal, D., additional, Ferrer, D., additional, Pabón, M., additional, Fernández-de-Mera, M.I.G., additional, Ruiz-Fons, F., additional, Alzaga, V., additional, Marco, I., additional, Calvete, C., additional, Lavin, S., additional, Gortazar, C., additional, López-Gatius, F., additional, and Dubey, J.P., additional

Published
2007
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249. Toxoplasmosis in sentinel chickens (Gallus domesticus) in New England farms: Seroconversion, distribution of tissue cysts in brain, heart, and skeletal muscle by bioassay in mice and cats.

Author

Dubey, J.P., Lehmann, T., Lautner, F., Kwok, O.C.H., and Gamble, H.R.

Subjects
*TOXOPLASMOSIS in animals, *CHICKEN diseases, *SEROCONVERSION, *CYSTS (Pathology), *BIOLOGICAL assay, *DIAGNOSIS
Abstract

Free-range chickens are a good indicator of soil contamination with oocysts because they feed from the ground and they are also an important source of infection for cats that in turn shed oocysts after eating tissues of intermediate hosts. Little is known of the epidemiology of toxoplasmosis in chickens. In the present study 90 Toxoplasma gondii seronegative, sentinel chickens were placed on three (30 each) swine farms in New England in November, 2003. Chickens were bled monthly and their sera were tested for T. gondii antibodies by the modified agglutination test (MAT, cut-off 1:25). Chickens that seroconverted were euthanized and their tissues were bioassayed in mice, cats, or both. Over the course of the experiment (7 months), 31 of 71 chickens seroconverted (MAT 1:100 or higher). Tissues of 26 seropositive chickens were bioassayed in both cats and mice; viable T. gondii was isolated, by bioassay in mice, from hearts (whole) of all 26 chickens, brains (whole) of 3 chickens and leg muscles (25 g) of 11 chickens; 21 of 26 cats fed 250 g of muscle from seropositive chickens excreted T. gondii oocysts. Results indicated that the density of T. gondii in poultry muscle is low but heart is the tissue of choice for isolation of viable parasites. [ABSTRACT FROM AUTHOR]

Published
2015
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250. Experimental transmission of Cystoisospora felis-like coccidium from bobcat (Lynx rufus) to the domestic cat (Felis catus).

Author

Dubey, J.P., Houk, A.E., Verma, S.K., Calero-Bernal, R., Humphreys, J.G., and Lindsay, D.S.

Subjects
*BOBCAT, *COCCIDIA, *HOSTS (Biology), *BIRD populations, *OOCYSTS, *DOMESTIC animals
Abstract

Cystoisospora felis is an ubiquitous coccidian of cats. The domestic cat ( Felis catus ) is its definitive host and several mammalian and avian species are its optional intermediate/transport hosts. Nothing is known if it is transmissible to wild felids. In the present study C. felis -like oocysts were found in two naturally infected bobcats ( Lynx rufus ) from Pennsylvania. To study transmission of C. felis- like parasite from bobcats to domestic cats, sporulated oocysts of C. felis- like from one bobcat were orally inoculated into interferon gamma gene knockout (KO) mice, and 56 days later tissues of KO mice were fed to two coccidian-free cats; two littermate cats were uninoculated controls. The inoculated cats and controls were euthanized five and seven days later, and their small intestines were studied histologically. One inoculated cat excreted C. felis- like oocysts seven days post inoculation (p.i.) and was immediately euthanized. Mature schizonts, mature male and female gamonts, and unsporulated oocysts were found in the lamina propria of small intestine; these stages were morphologically similar to C. felis of domestic cats. No parasites were seen in histological sections of small intestines of the remaining three cats. The experiment was terminated at seven days p.i. (minimum prepatent period for C. felis ) to minimize spread of this highly infectious parasite to other cats. Although oocysts of the parasite in bobcats were morphologically similar to C. felis of domestic cats, the endogenous stages differed in their location of development. The bobcat derived parasite was located in the lamina propria of ileum whereas all endogenous stages of C. felis of domestic cats are always located in enterocytes of intestinal epithelium. Characterization of DNA isolated from C. felis -like oocysts from the donor bobcat revealed that sequences of the ITS1 region was only 87% similar to the ITS1 region of C. felis from domestic cats. These results indicate that the parasite in bobcat is likely different than C. felis of cats. [ABSTRACT FROM AUTHOR]

Published
2015
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