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The aim of this study was to evaluate the influence of ethylene-releasing (ACC, Ethephon, Methionine) and -inhibiting (cobalt chloride, silver thiosulfate) compounds on ethylene production and shoot organogenesis of nodal segments, where buds were completely removed, from mature tissues of Citrus limon, Fino 49 and Verna 51 cultivars. The addition of ACC to the culture medium produced a very significant decrease of the regeneration. These results were directly related to the ethylene levels measured in the atmosphere inside the tube. Similar results were observed with ethephon and methionine; the gradual increase in ethylene levels in the tubes, with increasing ethylene-releasing compounds in the culture medium, agrees with the decrease in the regeneration rate observed, but the effect was lower than with ACC. When cobalt chloride (CoCl2) was added to the culture medium, contrary to what was expected, the regeneration decreased in both cultivars and this decreasing was not related with the increase in ethylene production. These observations matched the occurrence of yellowish necrotic explants increasing the concentration of cobalt chloride, probably because of a toxic effect on lemon explants. The increase of silver thiosulfate (STS) in the culture medium enhanced the regeneration percentage in both Verna 51 and Fino 49 cultivars. Nevertheless, ethylene levels increased proportionally with the STS concentration and the regeneration rate. These results may be explained since the effects produced by the high ethylene levels measured in STS experiments were blocked by Ag+ ions, thereby increasing the regeneration percentage. Media to which STS was added produced the highest regeneration percentages. The results obtained in this study showed that ethylene plays an important role in the organogenesis of mature explants of C. limon.

Plants are able to acquire induced resistance to pathogens (priming) by its previous exposure to biotic or abiotic stresses. To analyze whether this process is involved in the maize infection by Ustilago maydis , we have compared the infection occurring in plants inoculated under axenic conditions or in sterile soil to plants grown in non-sterile soil. Our results showed that plants grown under axenic conditions were more susceptible to infection than those inoculated in non-sterile soil. Accordingly, disease symptoms: chlorosis development, anthocyanin production, tumor development, and necrosis, were more and severe in axenic plants. In addition, cell death and reactive oxygen species production, as well as ethylene, were higher in axenic plants. These observations indicate for the first time, that different physical stressors and contact with microorganisms of the environment are responsible for the induction of resistance (priming) in this pathosystem.

Background: Sweet cherries are much appreciated by consumers as a result of their organoleptic quality attributes and antioxidant properties, although they deteriorate rapidly after harvest. Different preharvest strategies have been carried out to increase their quality at the time of harvest. We present data regarding the effect of preharvest salicylic acid (SA) and acetyl salicylic acid (ASA) treatments on sweet cherry quality during postharvest storage.; Results: At harvest and during postharvest storage, sweet cherry fruits ('Sweet Heart', 'Sweet Late' and 'Lapins') from SA (0.5 mmol L-1 ) and ASA (1 mmol L-1 ) treated trees had a higher colour (lower chroma index), firmness, total soluble solids, total phenolics, total anthocyanins and hydrophilic total antioxidant activity. In addition, the activity of the antioxidant enzymes catalase, peroxidase, superoxide dismutase and ascorbate peroxidase was also enhanced in SA- and ASA-treated cherries.; Conclusion: Both SA and ASA preharvest treatments could be promising tools for improving sweet cherry quality at harvest and after storage, with an additional effect on delaying the postharvest ripening process by increasing the levels of antioxidant compounds and the activity of the antioxidant enzymes. © 2016 Society of Chemical Industry.; © 2016 Society of Chemical Industry.

Objectives:Although there is general agreement on the characteristic features of the acute respiratory distress syndrome, we lack a scoring system that predicts acute respiratory distress syndrome outcome with high probability. Our objective was to develop an outcome score that clinicians could easi

The open lung approach is a mechanical ventilation strategy involving lung recruitment and a decremental positive end-expiratory pressure trial. We compared the Acute Respiratory Distress Syndrome network protocol using low levels of positive end-expiratory pressure with open lung approach resulting in moderate to high levels of positive end-expiratory pressure for the management of established moderate/severe acute respiratory distress syndrome.A prospective, multicenter, pilot, randomized controlled trial.A network of 20 multidisciplinary ICUs.Patients meeting the American-European Consensus Conference definition for acute respiratory distress syndrome were considered for the study.At 12-36 hours after acute respiratory distress syndrome onset, patients were assessed under standardized ventilator settings (FIO2≥0.5, positive end-expiratory pressure ≥10 cm H2O). If Pao2/FIO2 ratio remained less than or equal to 200 mm Hg, patients were randomized to open lung approach or Acute Respiratory Distress Syndrome network protocol. All patients were ventilated with a tidal volume of 4 to 8 ml/kg predicted body weight.From 1,874 screened patients with acute respiratory distress syndrome, 200 were randomized: 99 to open lung approach and 101 to Acute Respiratory Distress Syndrome network protocol. Main outcome measures were 60-day and ICU mortalities, and ventilator-free days. Mortality at day-60 (29% open lung approach vs. 33% Acute Respiratory Distress Syndrome Network protocol, p = 0.18, log rank test), ICU mortality (25% open lung approach vs. 30% Acute Respiratory Distress Syndrome network protocol, p = 0.53 Fisher's exact test), and ventilator-free days (8 [0-20] open lung approach vs. 7 [0-20] d Acute Respiratory Distress Syndrome network protocol, p = 0.53 Wilcoxon rank test) were not significantly different. Airway driving pressure (plateau pressure - positive end-expiratory pressure) and PaO2/FIO2 improved significantly at 24, 48 and 72 hours in patients in open lung approach compared with patients in Acute Respiratory Distress Syndrome network protocol. Barotrauma rate was similar in both groups.In patients with established acute respiratory distress syndrome, open lung approach improved oxygenation and driving pressure, without detrimental effects on mortality, ventilator-free days, or barotrauma. This pilot study supports the need for a large, multicenter trial using recruitment maneuvers and a decremental positive end-expiratory pressure trial in persistent acute respiratory distress syndrome.

ResumenEs indiscutible el creciente interés que están despertando las cuestiones asociadas a la responsabilidad social (RSE), no solo desde el plano académico sino también desde una dimensión social. En este contexto pretendemos, con el presente trabajo, analizar el nivel de desarrollo y de desempeño que las pequeñas y medianas empresas pueden tener en relación con las actividades de RSE. Igualmente, se pretende indagar sobre la relación que estas actividades pueden guardar con diversos factores organizativos como diversidad de género, nivel de formación del propietario/gerente, estructura de la propiedad, el tamaño y el sector. Los resultados, de carácter exploratorio, permiten sostener que las empresas españolas tienen una actitud positiva hacia la RSE y que, por término medio, se involucran en numerosas actividades socialmente responsables, siendo el nivel de formación del gerente/propietario, el tamaño y el sector los factores que más contribuyen en la explicación del desempeño socialmente responsable de las Pymes.AbstractThere is, without a doubt, an increasing awareness of the questions associated with Corporate Social Responsibility (CSR), not only from an academic point of view, but also from a social dimension. Within this framework, this paper analyzes the level of development of CSR in Small and Medium Sized Enterprises, and the relationship that this level of performance may have on organizational factors such as gender, educational level of the owner/manager, the ownership structure, size and industry. The findings of this exploratory study support the view that Spanish companies have a positive attitude towards CSR and they seem to be engaged in a numerous of socially responsible activities, with size, industry and the educational level of the owner/manager being determinant factors for its development.

Monoterpenes have antimicrobial properties but are associated with strong smells and flavors that limit their use in foods; therefore, strategies to keep their effectivity using lower concentrations are required. This work tested the antimicrobial capacity of thymol, carvacrol and linalool free or complexed in hydroxypropyl-β-cyclodextrins (HP- β-CDs) using two complexation methods. To this, these monoterpenes were complexed in HP- β-CDs by the solubility method or a microwave-assisted method, spray dried and their antimicrobial capacity was tested on Escherichia coli and Staphylococcus aureus by determining minimal inhibitory concentration and minimal bactericidal concentration. The results show significant differences (p

Plums are much appreciated by consumers as fresh fruit but have a limited storage life. Since reactive oxygen species (ROS) are associated with fruit ripening, an increase in ROS scavenging antioxidant systems could lead to a delay in postharvest plum ripening and in maintaining fruit quality after long cold storage. RESULTS Results showed that crop yield (kg per tree) and fruit weight were enhanced by preharvest oxalic acid (OA) treatment of plum cultivars (?Black Splendor? and ?Royal Rosa?), although the on-tree ripening process was delayed. In addition, the ripening process during cold storage was delayed in plums from OA-treated tress, manifested by lower firmness and acidity losses and reduced ethylene production, as compared with fruits from control trees. Antioxidant compounds (phenolics, anthocyanins and carotenoids) and the activity of antioxidant enzymes were higher in plums from OA-treated trees than in controls, at harvest and during 50 days of cold storage. CONCLUSION OA preharvest treatment could be a useful tool to maintain plum quality properties during long-term storage, by delaying the postharvest ripening process through a delay in ethylene production, with an additional effect on increasing bioactive compounds with health beneficial effects. ? 2018 Society of Chemical Industry

Objectives Overall mortality in patients with acute respiratory distress syndrome is a composite endpoint because it includes death from multiple causes. In most acute respiratory distress syndrome trials, it is unknown whether reported deaths are due to acute respiratory distress syndrome or the underlying disease, unrelated to the specific intervention tested. We investigated the causes of death after contracting acute respiratory distress syndrome in a large cohort. Design A secondary analysis from three prospective, multicenter, observational studies. Setting A network of multidisciplinary ICUs. Patients We studied 778 patients with moderate-to-severe acute respiratory distress syndrome treated with lung-protective ventilation. Interventions None. Measurements and main results We examined death in the ICU from individual causes. Overall ICU mortality was 38.8% (95% CI, 35.4-42.3). Causes of acute respiratory distress syndrome modified the risk of death. Twenty-three percent of deaths occurred from refractory hypoxemia due to nonresolving acute respiratory distress syndrome. Most patients died from causes unrelated to acute respiratory distress syndrome: 48.7% of nonsurvivors died from multisystem organ failure, and cancer or brain injury was involved in 37.1% of deaths. When quantifying the true burden of acute respiratory distress syndrome outcome, we identified 506 patients (65.0%) with one or more exclusion criteria for enrollment into current interventional trials. Overall ICU mortality of the "trial cohort" (21.3%) was markedly lower than the parent cohort (relative risk, 0.55; 95% CI, 0.43-0.70; p Conclusions Most deaths in acute respiratory distress syndrome patients are not directly related to lung damage but to extrapulmonary multisystem organ failure. It would be challenging to prove that specific lung-directed therapies have an effect on overall survival.

Podeu consultar la versió catalana al recurs relacionat., User guide to written communication in academic disciplines (in this case law) for teachers and students.

Sweet cherry trees ( Prunus avium L.) were treated with methyl salicylate (MeSa) at 1 mM at 3 key events on fruit development on-tree to analyze the effect of MeSa on bioactive compounds, total antioxidant activity (TAA) and antioxidant enzymes at harvest and during 28 days storage. The experiments were performed during two consecutive years, by using two sweet cherry cultivars, ‘Sweet Heart’ and ‘Sweet Late’ in 2013 and another more cultivar, ‘Lapins’, in 2014. Both total phenolics and anthocyanins content were significantly higher in MeSa-treated than in control fruit at harvest and during storage, leading to fruit with higher hydrophilic TAA (H-TAA). The activity of the antioxidant enzymes catalase (CAT), peroxidase (POD), ascorbate peroxidase (APX) and superoxide dismutase (SOD) decreased along storage for all cultivars and experimental years, although those fruit treated with MeSa exhibited higher activities of these antioxidant enzymes than controls. Thus, MeSa treatment of cherry trees could increase health-promoting properties of cherry fruit consumption, due to its effect on increasing antioxidant and bioactive compounds, with additional effect on delaying the fruit postharvest senescence process by increasing the activity of the enzymes involved in reactive oxygen species (ROS) scavenging.

The application of nanomaterials in commercially available products is increasing rapidly for agriculture, phytoremediation and biotechnology. Since plants suppose the first sink for the accumulation of nanoparticles from the environment, emerging studies have focused on the general consequences for plants and their effects on the biomass production. However, effects on the root surface, as well as blockage of nutrients and water uptake by the roots, may also occur. This experiment was designed to prove if the plant water relations can be affected by the adsorption of nanoparticles on the root surface, causing a consequent stress for the plants. With this goal, plants of Helianthus annuus were previously grown in a hydroponic culture, and at age of 55 days, their roots were exposed to three different concentrations of nanomaghemite (NM) in the hydroponic solution for 5 days: control without NM; 50 and 100 mg l(-1) NM. The main effect was related to the reduction of the root hydraulic conductivity (Lo) and the nutrients uptake. The concentrations of the macronutrients Ca, K, Mg and S in the shoot were reduced relative to the control plants, which resulted in lower contents of chlorophyll pigments. Although stress was not detected in the plants, after the analysis of stress markers like the accumulation of proline or ascorbate in the tissues, reduction of the root functionality by nanoparticles has been identified here, manifested as the effect of NM on Lo. The treatment with 50 mg l(-1) NM significantly reduced the Lo, by up to 57% of its control value, and it was reduced by up to 26% at 100 mg l(-1) NM. These results will be an important factor to take into account with regard to the applicability of NM for long-term use in crops, particularly during privative water conditions.

The increasing social concern about the protection and improvement of the environment has transformed environmental commitment into a new strategic via channel through which to achieve competitiveness in firms. Nevertheless, to date, limited research on this subject has focused on small and medium enterprises. This study addresses this gap by shedding light on the relationship between environmental commitment and business performance as well as their relationship with two other strategic variables: relational marketing and image. From a sample of 481 small and medium firms in Southern Spain and using structural equation modelling, different cause–effect relations were analysed. The results suggest that environmental performance has a positive, direct and significant influence on competitive performance as well as the mediating effects of image and relational marketing. In this regard, this paper offers new empirical evidence of how firms that improve their environmental engagement generate a positive interaction with their stakeholders. An improved environmental performance encourages the appearance of positive reactions for any company's image and its competitiveness. Some of these environmental practices are provided according to their strategic relevance.

ResumenEn este trabajo se describe la evolución de la investigación en responsabilidad social en pequeñas y medianas empresas durante el período 1976-2013. Hemos recopilado una base de datos de los 321 artículos centrados en el campo publicados en revistas incluidas en el Social Science Citation Index. Se hace uso de métodos y técnicas bibliométricas para describir la evolución de la actividad científica, los países e instituciones activos, autores más productivos, las metodologías aplicadas y los principales temas investigados con el objetivo de determinar el estado y describir la evolución de la literatura en este campo. Sobre la base de estos análisis, se proponen posibles vías para futuras investigaciones para avanzar su consolidación como disciplina científica.AbstractThis paper describes the evolution of the corporate social responsibility in small and medium enterprises research over the period 1976-2013. A database has been complied of the 321 articles focused on this field published in journals included in the Social Science Citation Index. Bibliometric methods and techniques are used to describe the evolution of scientific activity, the most active countries and institutions, the most productive authors, the methodologies and the main topics investigated in order to describe trends, and the status and evolution of literature in this field. Based on these analyses, potential avenues for future research are proposed to advance the consolidation of the field as a scientific discipline.

Breast cancer is the most common type of cancer in females in Argentina, with an incidence rate similar to that in the USA. However, the contribution of the BRCA1 or BRCA2 mutation in breast cancer incidence has not yet been investigated in Argentina. In order to evaluate which BRCA1 polymorphisms or mutations characterize female breast cancer in Argentina, the current study enrolled 206 females with breast cancer from several hospitals from the southeast of Argentina. A buccal smear sample was obtained in duplicate from each patient and the DNA samples were processed for polymorphism analysis using the single-strand conformational polymorphism technique. The polymorphisms in BRCA1 were investigated using a combination of 15 primers to analyze exons 2, 3, 5, 20 and 11 (including the 11.1 to 11.12 regions). The BRCA1 mutations were confirmed by direct sequencing. Samples were successfully examined from 154 females and, among these, 16 mutations were identified in the BRCA1 gene representing 13.9% of the samples analyzed. One patient was identified with a polymorphism in exon 2 (0.86%), four in exon 20 (3.48%), four in exon 11.3 (3.48%), one in exon 11.7 (0.86%), two in exon 11.8 (1.74%), one in exon 11.10 (0.86%) and one in exon 11.11 (0.86%). The most prevalent alteration in BRCA1 was located in exon 11 (11 out of 16 patients; 68.75%). The objective of our next study is to evaluate the prevalence of mutations in the BRCA2 gene and analyze the BRCA1 gene in the healthy relatives of BRCA1 mutation carriers. Fil: Jaure, Omar David Argentino. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina Fil: Alonso, Eliana Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina Fil: Aguilera Braico, Diego Máximo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina Fil: Nieto, Alvaro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina Fil: Orozco, Manuela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina Fil: Morelli, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina Fil: Ferro, Alejandro M.. Hospital Italiano; Argentina Fil: Barutta, Elena. Medifem; Argentina Fil: Vincent, Esteban. Medifem; Argentina Fil: Martínez, Domingo. Provincia de Buenos Aires. Ministerio de Salud. Hospital Municipal de Agudos Dr. Leónidas Lucero; Argentina Fil: Martínez, Ignacio. Provincia de Buenos Aires. Ministerio de Salud. Hospital Municipal de Agudos Dr. Leónidas Lucero; Argentina Fil: Maegli, Maria Ines. Provincia de Buenos Aires. Ministerio de Salud. Hospital Municipal de Agudos Dr. Leónidas Lucero; Argentina Fil: Frizza, Alejandro. Medifem; Argentina Fil: Kowalyzyn, Ruben. Clínica Viedma; Argentina Fil: Salvadori, Marisa. Hospital Dr. Lucio Molas; Argentina Fil: Ginestet, Paul. Provincia de Buenos Aires. Ministerio de Salud. Hospital y Maternidad Municipal Pigüé; Argentina Fil: Gonzalez Donna, Maria L.. Hospital Italiano; Argentina Fil: Balogh, Gabriela Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina

During the phytorestoration of a soil contaminated by trace elements (TEs), the most appropriate combination of plant species and soil amendments has to be evaluated in order to increase the biomass production of plants and thus, the efficacy of the process. The response of three native species ( Dittrichia viscosa , Nicotiana glauca and Silybum marianum ), and the applicability of a compost made from pig slurry and a humic fertiliser (HF) prepared from it, were assessed in a mesocosm pot-experiment through the plant growth and composition. The compost reduced the TEs concentrations in the plants to a greater extent than the HF and increased plant biomass of the three studied species, whereas HF increased the TEs concentrations in the leaves of N. glauca and limited its growth. S. marianum showed a high biomass response and was able to regulate the uptake of As and Zn from the soil solution and their transport to the harvestable parts. Similarly, D. viscosa restricted the transfer of As from the soil to the leaves. Therefore, the use of compost and D. viscosa or S. marianum can be considered a good combination for the remediation by phytostabilisation of TEs contaminated soils under semiarid conditions.

‘Black Splendor’ (BS) and ‘Royal Rosa’ (RR) plums were treated preharvest with methyl jasmonate (MeJA) at three concentrations (0.5, 1.0 and 2.0 mM) along the on-tree fruit development: 63, 77 and 98 days after full blossom (DAFB). Both control and treated fruit were harvested at the commercial ripening stage and stored in two temperature conditions: 9 days at 20 °C or at 2 °C + 1 day at 20 °C for 50 days. Preharvest MeJA at 2.0 mM significantly accelerated whereas 0.5 mM delayed the postharvest ripening process for both cultivars, since ethylene production, respiration rate and softening were reduced significantly at the two storage conditions for 0.5 mM. In these fruit, total phenolics, total antioxidant activity (hydrophilic fraction, HTAA) and the antioxidant enzymes peroxidase (POD), catalase (CAT) and ascorbate peroxidase (APX) were found at higher levels in treated than control plums during postharvest storage, which could account for the delay of the postharvest ripening process and the extension of shelf-life.

Two plum ( Prunus salicina Lindl.) cultivars ‘Black Splendor’ (BS) and ‘Royal Rosa’ (RR) were treated with methyl jasmonate (MeJA) at 3 concentrations (0.5, 1.0 and 2.0 mM) along the on-tree fruit development: 63, 77 and 98 days after full blossom (DAFB). On a weekly basis, fruit samples were taken for measuring fruit size and weight and parameters related to quality. Results revealed that MeJA was effective in increasing fruit size and weight, the 0.5 mM being the most effective for BS cultivar and 2.0 mM for RR. At harvest, those fruit treated with 0.5 mM MeJA had the highest firmness and colour Hue values. Total acidity was also generally higher in MeJA-treated fruit than in controls, while the content of total soluble solids remained unaffected. In addition, total phenolics and total antioxidant activity were found at higher concentrations in 0.5 and 2.0 mM MeJA-treated than in control fruit over at last 3 weeks of fruit development for BS and RR cultivars, respectively. Overall results suggest that MeJA could be a promising preharvest tool to increase plum size and quality with enhanced bioactive compounds and antioxidant activity, although the optimum concentration is cultivar dependent.

Previous reports have addressed the effectiveness of salicylic acid (SA), acetylsalicylic acid (ASA) and methylsalicylate (MeSA) postharvest treatments on maintaining quality properties during storage in several commodities. However, there is no literature regarding the effect of preharvest treatments with salicylates on plum quality attributes (at harvest or after long-term cold storage), which was evaluated in this research.At harvest, weight, firmness, individual organic acids, sugars, phenolics, anthocyanins and total carotenoids were found at higher levels in plums from SA-, ASA- and MeSA-treated trees than in those from controls. During storage, softening, colour changes and acidity losses were delayed in treated fruits as compared to controls. In addition, organic acids and antioxidant compounds were still found at higher levels in treated than in control plums after 40 days of storage. Results show a delay in the postharvest ripening process due to salicylate treatments, which could be attributed to their effect in delaying and decreasing ethylene production.Preharvest treatment with salicylates could be a safety, eco-friendly and new tool to improve (at harvest) and maintain (during storage) plum quality and especially its content of bioactive compounds with antioxidant properties, increasing the health effects of plum consumption. © 2017 Society of Chemical Industry.

[Purpose] The presence of high concentrations of trace elements (TEs) in mine soils like those in the Sierra Minera of La Unión-Cartagena (SE Spain) limits the development of a vegetation cover on such sites, and pollution dispersion by water and wind erosion represents a serious risk for the surrounding ecosystems. The aim of this study was to evaluate different phytostabilisation procedures based on the co-culture of a legume (Bituminaria bituminosa) and a high-biomass (Piptatherum miliaceum) species for this type of soils., [Materials and methods] A pot experiment was carried out where B. bituminosa was tested as a soil pre-treatment strategy. Five different procedures were followed to study the growth stimulation or competition of both species in a contaminated soil from the Sierra Minera: (i) sowing of P. miliaceum without B. bituminosa (control treatment), (ii) sowing of P. miliaceum for co-cultivation of both species, (iii) sowing of P. miliaceum and co-cultivation of both species in soil with compost, (iv) harvesting and elimination of the aerial part of the plants before sowing of P. miliaceum and (v) harvesting and incorporation to the soil of the aerial part of B. bituminosa before sowing of P. miliaceum., [Results and discussion] The results showed that the co-culture of both species favoured the growth of P. miliaceum, whilst incorporating the aerial part of the legume to the soil increased nitrogen concentration in P. miliaceum but reduced its growth. The use of compost improved both the growth and N uptake of P. miliaceum and did not inhibit nodulation in B. bituminosa. TE extractability in the soils and accumulation in the plants were rather low and very little affected by the addition of the amendments or by co-culture of species., [Conclusions] Nitrogen availability plays an important role in P. miliaceum growth in TE-contaminated mine soils. The addition of compost together with legume cultivation is proposed as an effective combination for the cultivation of P. miliaceum in these soils, as both plant growth and soil conditions were improved following this procedure., This work was financed by the Spanish “Ministerio de Economía y Competitividad” (MINECO) and the EU through FEDER funds (projects CTM2010-21922-C02-01 and CTM2013-48697-C02-1R). Part of R. Clemente’s salary is paid by the European Social Fund of the European Union. E. Arco-Lázaro thanks the Spanish MINECO for the FPI grant awarded (ref.: BES-2011-050608). The European project Postdok CZU (ESF/MŠMT CZ.1.07/2.3.00/30.0040) and the assistance of Dr David J. Walker for English revision are also acknowledged.

The driving pressure (plateau pressure minus positive end-expiratory pressure) has been suggested as the major determinant for the beneficial effects of lung-protective ventilation. We tested whether driving pressure was superior to the variables that define it in predicting outcome in patients with acute respiratory distress syndrome.A secondary analysis of existing data from previously reported observational studies.A network of ICUs.We studied 778 patients with moderate to severe acute respiratory distress syndrome.None.We assessed the risk of hospital death based on quantiles of tidal volume, positive end-expiratory pressure, plateau pressure, and driving pressure evaluated at 24 hours after acute respiratory distress syndrome diagnosis while ventilated with standardized lung-protective ventilation. We derived our model using individual data from 478 acute respiratory distress syndrome patients and assessed its replicability in a separate cohort of 300 acute respiratory distress syndrome patients. Tidal volume and positive end-expiratory pressure had no impact on mortality. We identified a plateau pressure cut-off value of 29 cm H2O, above which an ordinal increment was accompanied by an increment of risk of death. We identified a driving pressure cut-off value of 19 cm H2O where an ordinal increment was accompanied by an increment of risk of death. When we cross tabulated patients with plateau pressure less than 30 and plateau pressure greater than or equal to 30 with those with driving pressure less than 19 and driving pressure greater than or equal to 19, plateau pressure provided a slightly better prediction of outcome than driving pressure in both the derivation and validation cohorts (p0.0000001).Plateau pressure was slightly better than driving pressure in predicting hospital death in patients managed with lung-protective ventilation evaluated on standardized ventilator settings 24 hours after acute respiratory distress syndrome onset.

Nanomaterials, including engineered nano-sized iron oxides, manganese oxides, cerium oxides, titanium oxides, or zinc oxides, provide specific affinity for metal/metalloids adsorption and their application is being rapidly extended for environmental management. Their significant surface area, high number of active surface sites, and high adsorption capacities make them very promising as cost-effective amendments for the remediation of contaminated soils. The alleviation of the toxicities of metal/metalloids by their immobilization in the soil stimulates the growth and development of plants during phytoremediation, but there is a body of evidence indicating that nanomaterials themselves can yield both beneficial and harmful effects in plant systems at the physiological, biochemical, nutritional, and genetic levels. Nanoecotoxicological studies are providing a good understanding of their interactions with plants, and an increasing number of publications have attempted to clarify and quantify their potential risks and consequences for plants. However, many results are contradictory and the safety of engineered nanomaterials still represents a barrier to their wide, innovative use in phytoremediation. Both their positive and negative effects on plants will have to be taken into account to evaluate their applicability, and the scientific community faces a challenge to understand deeply the factors which can determine their relevance in environmental science and technology.

The effect of oxalic acid (OA) on the overall quality of artichokes ( Cynara cardunculus L. subsp. scolymus (L.) Hayek, formerly Cynara scolymus L. cv. Blanca de Tudela) was investigated for the first time in this vegetable. Artichokes were dipped in OA solution at 1 mM for 10 min and then stored at 20 °C. OA application delayed the deterioration process in artichokes during storage by retarding weight loss, softening, colour changes and chlorophyll degradation. In addition OA significantly reduced mesophilic aerobics and yeast and moulds counts with respect to controls. Finally, the content of total phenolics and antioxidant activity were not affected negatively by OA treatment. Thus, OA treatment might be a promising method for delaying postharvest deterioration and maintaining the overall quality of artichokes.

Two experiments were carried out to study the competition for adsorption between trace elements (TEs) and nutrients following the application of nano-maghemite (NM) (iron nano-oxide; Fe2O3) to a soil solution (the 0.01 mol L−1 CaCl2 extract of a TEs-contaminated soil). In the first, the nutrients K, N, and P were added to create a set of combinations: potential availability of TEs during their interaction with NM and nutrients were studied. In the second, response surface methodology was used to develop predictive models by central composite design (CCD) for competition between TEs and the nutrients K and N for adsorption onto NM. The addition of NM to the soil solution reduced specifically the concentrations of available As and Cd, but the TE-adsorption capacity of NM decreased as the P concentration increased. The CCD provided more concise and valuable information, appropriate to estimate the behavior of NM sequestering TEs: according to the suggested models, K+ and NH4+ were important factors for Ca, Fe, Mg, Mn, Na, and Zn adsorption ( R a d j 2 = 95 % , except for Zn with R a d j 2 = 87 % ). The obtained information and models can be used to predict the effectiveness of NM for the stabilization of TEs, crucial during the phytoremediation of contaminated soils.

In this work Aloe vera gel (AV) alone or with the addition of 10 or 2% rosehip oil was used as fruit edible coatings in a wide range of Prunus species and cultivars: peaches (‘Roma’ and ‘B-424-16’ flat type), plums (‘Red Beauty’ and ‘Songria’), nectarine (‘Garofa’) and sweet cherry (‘Brooks’). Following treatments, fruit were stored at 20 °C for 6 days and analysed for the effect of treatments on fruit ripening and quality parameters compared with uncoated fruit (control). The addition of the rosehip oil to AV gel reduced respiration rate in all fruit, and ethylene production in the climacteric ones (peaches, plums and nectarine). In addition, all the parameters related with fruit ripening and quality, such as weight loss, softening, colour change and ripening index, were also delayed in treated compared with control fruit, the effect being generally higher when rosehip oil was added to AV, and especially in those fruit that exhibited the highest ethylene production rates (‘Roma’ and flat type peaches). Although the highest effect was obtained with AV + rosehip oil at 10%, the sensory panel detected an excess of gloss and oiliness on the fruit surface, which was considered as a negative attribute. Thus, 2% rosehip oil added to AV could be used as an innovative postharvest tool to increase the beneficial effect of AV as an edible coating, especially in climacteric fruit showing high ethylene production rates.

The role and impact of small and medium enterprises (SMEs) in the economy means that it is becoming increasingly difficult to dismiss the importance of the latter in socially relevant issues as important as employment, innovation and standard of living. Through content analysis, this study mainly provides a literature survey about the concept of Corporate Social Responsibility (CSR) in relation to quality management in SMEs. It seeks to identify main CSR practices SMEs develop, and investigate if they are related to quality management practices directly or at least implicitly. This would help us to identify parallels between quality management and the importance of conducting socially responsible management. Using 107 empirical studies from 1976 to September 2014, this study provides a baseline for future empirical studies in these areas. For this purpose, main topics and practices of CSR in the SME context are identified. Specifically, we arrive at the conclusion that these firms are increasingly integrating both CSR and quality management practices to comply with stakeholders needs, and that most common social and environmental practices facilitate the development of quality management practices.

Several postharvest treatments were performed on pomegranate arils prior to storage in rigid polypropylene boxes for 12 days at 3 °C: water (control), ascorbic + citric acids (at 0.5 or 1%), Aloe vera gel (at 50 or 100%), 50% A. vera gel + 0.5% ascorbic and 0.5% citric acid, and 100% A. vera gel + 1% ascorbic and 1% citric acid. A. vera (alone or in combination with acids) led to lower CO2 and higher O2 concentrations inside the packages compared with arils treated with water (control). With respect to quality attributes, A. vera coatings led to firmness retention and increased levels of total anthocyanins and total phenolics. In addition, A. vera treatments led to significantly lower counts for both mesophilic aerobics and yeast and moulds. Sensory analysis scores for flavour, texture, aroma, colour and purchase decision were higher in arils treated with A. vera, especially in those arils treated with 100% A. vera + 1% ascorbic and citric acids. Finally, no off-flavours in pomegranate arils were perceived by judges as a consequence of A. vera gel treatment.

Recently harvested peaches and plums were coated with either Aloe vera or Aloe arborescens gels and allowed to ripen at 20 °C for six days. Both coatings significantly delayed ethylene production, the effect being higher in plum which had the highest ethylene production rates. Changes in quality parameters related to peach and plum postharvest ripening, such as colour changes, reduction of acidity and increasing in ripening index (total soluble solids/total acidity ratio), were significantly delayed in coated fruit. In addition, both coatings significantly reduced weight loss, especially the A. arborescens gel. Thus, A. arborescens gel could be even more effective than A. vera gel for use as an edible coating for preserving the quality of climacteric fruit.

Pepper plants ( Capsicum annuum L. cv Lamuyo) were treated with a mix of nitrophenolates either by foliar spray or in the irrigation system, and its effect on crop yield and the content of nutritive and bioactive compounds in fruit were analysed at harvest and after post-harvest storage. Treatments were applied at 2-week intervals from the development of first floral bunch (1 March) to end of August. Pepper fruits were harvested at commercial ripening stage (red surface colour) along the growth cycle (from May to September). Total yield (cumulative kilogram per plant) was increased (≈4.5% more) by nitrophenolate treatments due to significant increases in fruit mass, although the number of fruits per plant was unaffected. Pepper fruit quality (weight, firmness and pericarp thickness), its content in nutritive (sugars and organic acids) and bioactive compounds (vitamin C and total phenolics) and antioxidant activity were also enhanced by nitrophenolate treatments at the three harvested dates assayed (end May, mid July and end August). In addition, all these parameters were maintained at higher levels in treated peppers during storage, while diminutions in these parameters occurred generally in control fruit. Thus, nitrophenolate treatments were able to improve crop yield as well as the nutritional value and antioxidant properties of peppers at harvest and after fruit storage.

The leaf characteristics and gel chemical composition of eight Aloe species (Aloe arborescens Mill., Aloe aristata Haw., Aloe claviflora Strydenburg, Aloe ferox Mill., Aloe mitriformis Mill., Aloe saponaria Ait., Aloe striata Haw., and Aloe vera L.) were studied in freshly harvested leaves at three different seasons within the Mediterranean climate: winter, spring and summer. Results revealed that differences existed in leaf properties and chemical composition of the gels of the several Aloe spp. and harvest seasons. The highest gel percentage was obtained from A. vera and A. claviflora (≈62–65%) followed by A. ferox and A. mitriformis (≈50–58%). Harvest season greatly affected gel properties with increases in lipids, proteins, aloin, total phenolics, total antioxidant activity (hydrophilic and lipopihilic fractions) and polyamines (putrescine and spermidine) were obtained in the summer season, whilst they were no differences in leaf characteristics. In addition, the growth potential of fruit pathogenic fungi (Botrytis cinerea, Penicillium digitatum, Penicillium expansum and Penicillium italicum), artificially inoculated on the whole leaves, was evaluated. The highest antifungal activity, measured as absence of or low percentage of infected wounds, was obtained for A. ferox, A. mitriformis and A. saponaria, this antifungal activity being positively correlated with gel aloin content. A. ferox, A. mitriformis and A. saponaria could be good alternatives to A. vera for commercial gels and other practical applications.

Four plum (Prunus salicina Lindl.) cultivars (“Blackamber”, “Larry Ann”, “Golden Globe” and “Songold”), were treated with 1 or 3% alginate as an edible coating before storage. Analytical determinations were made after 7, 14, 21, 28 and 35 days at 2 °C and after a 3 day period at 20 °C (shelf-life). Both treatments were effective in inhibiting ethylene production for all cultivars, especially when 3% alginate was used. The changes in fruit quality parameters related to plum postharvest ripening, such as weight and acidity losses, softening and colour changes, were significantly delayed by the use of both edible coatings. The delay of the ripening process was also related to lower anthocyanin and carotenoid accumulation. Overall results suggest that these treatments could increase the plum storage period with optimum quality, 2 weeks for “Larry Ann” and “Songold” and 3 weeks for “Blackamber” and “Golden Globe” more than controls.

In this paper, the effects of ultraviolet (UV) treatments on the aloin content of Aloe vera L. gel have been analyzed. UV-A treatment to A. vera plants for 36 days led to an increase in the aloin concentration in gel, rind tissue, and latex, while a decrease in chlorophylls a and b occurred in the photosynthetic tissue as a consequence of UV treatment. The growth of Penicillium digitatum and Botrytis cinerea (artificially inoculated on the leaf surface) was drastically decreased in UV-A-treated leaves, which could be attributed to the increase in the aloin concentration by the UV-A treatment. In addition, UV-C treatment to detached leaves also led to an increase in the gel aloin concentration, at higher levels than occurred with UV-A treatment, although leaves showed severe lesions after 48 h of treatment.