Search

Your search keyword '"Doekes, G."' showing total 772 results
Start Over Author "Doekes, G."
772 results on '"Doekes, G."'

203. Reply

Author

Krop, E. J. M., primary, Doekes, G., additional, Heederik, D. J. J., additional, Aalberse, R. C., additional, and van der Zee, J. S., additional

Published
2011
Full Text
View/download PDF

205. IgG to Various Beta-Glucans in a Human Adult Population

Author

Noss, I., Wouters, I.M., Smit, L.A., Meijer, E., Pronk, A., Heederik, D.J.J., Doekes, G., Risk Assessment of Toxic and Immunomodulatory Agents, and Dep IRAS

Subjects
Adult, Male, beta-glucan, beta-Glucans, Immunology, Coronacrisis-Taverne, Air Pollutants, Occupational, macromolecular substances, Cross Reactions, Normal human sera, Pustulan, Laminarin, Immunoglobulin G, Baker’s yeast glucan, Microbiology, chemistry.chemical_compound, Antigen, Antibody Specificity, Occupational Exposure, medicine, Immunology and Allergy, Humans, Beta (finance), biology, medicine.diagnostic_test, Lung Diseases, Fungal, Fungi, General Medicine, Middle Aged, carbohydrates (lipids), Titer, stomatognathic diseases, medicine.anatomical_structure, chemistry, Immunoassay, biology.protein, IgC, Antibody, Respiratory tract, Protein Binding
Abstract

Background: Fungal β-(1,3)-glucans are pro-inflammatory agents, and exposures to β-(1,3)-glucans are associated with respiratory tract symptoms. IgG anti-(1,3)-glucan titers are measured in diagnosis of fungal infections. Although other β-glucan structures exist, like β-(1,6)-glucans, little is known about their antigenic or pro-inflammatory properties. We aimed to investigate IgG titers and specificities in human sera against different β-glucans with varying structures. Methods: IgG anti-β-glucan was measured by enzyme immunoassay in a random sample of 40 sera from healthy adults, with a panel of 8 differently structured glucans. In a subsequent larger series, IgG anti-β-(1,6)-glucan was measured in a random sample of 667 sera from three occupational populations with different organic dust exposures. Possible determinants of IgG anti-β-(1,6)-glucan titers were explored with linear-regression analysis. Results: We found wide variation in anti-β-glucan IgG levels. The highest titers were found for pure β-(1,6)-glucan pustulan. Moderate to strong reactions with other β-(1,6)-containing structures appeared to be due to cross-reacting anti-β-(1,6)-glucan antibodies. Surprisingly, the mean IgG anti-β-(1,6)-glucan titer was significantly lower in agricultural workers – with highest organic dust exposure – than in spray painters and bakery workers. Smoking status was associated with lower IgG anti-β-(1,6)-glucan titers in all populations. Conclusions: IgG to β-(1,3)- and β-(1,6)-glucans can be found in normal human sera. β-(1,6)-glucans appear to be much more potent antigens. The health impact of high anti-β-(1,6)-glucan antibody levels remains unclear and further investigations are needed.

Published
2012

206. Lung function decline in laboratory animal workers: the role of sensitisation and exposure

Author

Portengen, L., Hollander, A., Doekes, G., de Meer, G., Heederik, D.J.J., Portengen, L., Hollander, A., Doekes, G., de Meer, G., and Heederik, D.J.J.

Abstract

Background: Little is known about the relation between allergic sensitisation and subsequent long term lung function changes in working populations exposed to sensitising agents. Aims: To investigate whether exposure and work related sensitisation to laboratory animals are associated with lung function decline. Methods: The relation between exposure and sensitisation to laboratory animal allergens and changes in lung function was investigated in a longitudinal study (median follow up 2.0 years) among 319 laboratory animal workers. Subjects who had been working with laboratory animals for less than 4 years (n = 102) were analysed separately, since an earlier cross sectional analysis had suggested a strong healthy worker effect in more experienced workers. Results: In multiple regression analyses both sensitisation and exposure appeared to contribute independently to lung function decline in subjects who had been working with laboratory animals for less than 4 years, adjusting for gender, age, smoking, and atopy. Lung function decline was most pronounced in sensitised subjects who continued to be in contact with the animals to which they were sensitised, with estimated average excess declines in FEV1, FVC, and MMEF of 83 ml/y (p < 0.05), 148 ml/y (p < 0.01), and 7 ml/s/y (p = 0.9). Conclusions: We conclude that exposure to laboratory animals is a significant risk factor for accelerated lung function decline, and that sensitised workers are especially at risk.

Published
2003

208. The development and use of a polyclonal sandwich enz:fme immuno assay in occupational exposure assessment of Natural Rubber Latex proteins

Author

Universiteit Utrecht, Faculteit Diergeneeskunde, Niftrik, M., Portengen, L., Tomazic-Jezic, V.J., Chen, Z., Tjoe Nij, E.I.M., de Wind, S., Heederik, D.J.J., Doekes, G., Universiteit Utrecht, Faculteit Diergeneeskunde, Niftrik, M., Portengen, L., Tomazic-Jezic, V.J., Chen, Z., Tjoe Nij, E.I.M., de Wind, S., Heederik, D.J.J., and Doekes, G.

Published
2003

212. Upper airway inflammation and respiratory symptoms in domestic waste collectors

Author

Universiteit Utrecht, Faculteit Diergeneeskunde, Wouters, I.M., Hilhorst, S., Kleppe, P., Doekes, G., Douwes, J.E., Peretz, C., Heederik, D.J.J., Universiteit Utrecht, Faculteit Diergeneeskunde, Wouters, I.M., Hilhorst, S., Kleppe, P., Doekes, G., Douwes, J.E., Peretz, C., and Heederik, D.J.J.

Published
2002

222. Passive airborne dust sampling with the electrostatic dustfall collector: optimization of storage and extraction procedures for endotoxin and glucan measurement

Author

Noss, I., Doekes, G., Sander, I., Heederik, D.J.J., Thorne, P.S., Wouters, I.M., Risk Assessment of Toxic and Immunomodulatory Agents, and Dep IRAS

Subjects
Dust sample, endotoxin, dust sampling, exposure assessment, Polyesters, Static Electricity, Air Microbiology, Polysorbates, indoor environment, Specimen Handling, Limit of Detection, Animals, Humans, passive sampling, Glucan, chemistry.chemical_classification, Air Pollutants, Chromatography, Chemistry, Textiles, Extraction (chemistry), lipopolysaccharide, Public Health, Environmental and Occupational Health, Dust, General Medicine, Aerosol, Endotoxins, glucans, Environmental chemistry, Air Pollution, Indoor, Housing, Regression Analysis, exposure assessment methodology, Quantitative analysis (chemistry), Filtration, Passive sampling, Environmental Monitoring
Abstract

We recently introduced a passive dust sampling method for airborne endotoxin and glucan exposure assessment-the electrostatic dustfall collector (EDC). In this study, we assessed the effects of different storage and extraction procedures on measured endotoxin and glucan levels, using 12 parallel EDC samples from 10 low exposed indoor environments. Additionally, we compared 2- and 4-week sampling with the prospect of reaching higher dust yields. Endotoxin concentrations were highest after extraction with pyrogen-free water (pf water) + Tween. Phosphate-buffered saline (PBS)-Tween yielded significantly (44%) lower levels, and practically no endotoxin was detected after extraction in pf water without Tween. Glucan levels were highest after extraction in PBS-Tween at 120 degrees C, whereas extracts made in NaOH at room temperature or 120 degrees C were completely negative. Direct extraction from the EDC cloth or sequential extraction after a preceding endotoxin extraction yielded comparable glucan levels. Sample storage at different temperatures before extraction did not affect endotoxin and glucan concentrations. Doubling the sampling duration yielded similar endotoxin and only 50% higher glucan levels. In conclusion, of the tested variables, the extraction medium was the predominant factor affecting endotoxin and glucan yields.

Published
2010

223. beta-(1,3)-Glucan exposure assessment by passive airborne dust sampling and new sensitive immunoassays

Author

Noss, I., Wouters, I.M., Bezemer, G., Metwali, N., Sander, I., Raulf-Heimsoth, M., Heederik, D.J.J., Thorne, P.S., Doekes, G., Risk Assessment of Toxic and Immunomodulatory Agents, and Dep IRAS

Subjects
Validation study, Antigens, Fungal, beta-Glucans, Population, Static Electricity, Air pollution, Air Microbiology, Coronacrisis-Taverne, Public Health Microbiology, medicine.disease_cause, Applied Microbiology and Biotechnology, complex mixtures, Immunoenzyme Techniques, medicine, Humans, education, Glucan, Exposure assessment, chemistry.chemical_classification, Inhalation exposure, education.field_of_study, Air Pollutants, Antigens, Bacterial, Inhalation Exposure, Ecology, medicine.diagnostic_test, Dust, Environmental exposure, Environmental Exposure, Antigens, Plant, Asthma, respiratory tract diseases, carbohydrates (lipids), stomatognathic diseases, chemistry, Immunoassay, Environmental chemistry, Air Pollution, Indoor, Housing, Proteoglycans, Food Science, Biotechnology, Environmental Monitoring
Abstract

Associations between house dust-associated β-(1,3)-glucan exposure and airway inflammatory reactions have been reported, while such exposures in early childhood have been suggested to protect against asthma and wheezing. Most epidemiological studies have used reservoir dust samples and an inhibition enzyme immunoassay (EIA) for β-(1,3)-glucan exposure assessment. The objective of this study was to develop inexpensive but highly sensitive enzyme immunoassays to measure airborne β-(1,3)-glucans in low-exposure environments, like homes. Specificities of available anti-β-(1,3)-glucan antibodies were defined by direct and inhibition experiments. Three suitable antibody combinations were selected for sandwich EIAs. β-(1,3)-Glucans in passive airborne dust collected with an electrostatic dust fall collector (EDC) and floor dust from seven homes were measured with the three EIAs. Floor dust samples were additionally analyzed in the inhibition EIA. The sandwich EIAs were sensitive enough for airborne glucan measurement and showed different specificities for commercial glucans, while the β-(1,3)-glucan levels in house dust samples correlated strongly. The feasibility of measuring glucans in airborne dust with the recently introduced EDC method was further investigated by selecting the most suitable of the three EIAs to measure and compare β-(1,3)-glucan levels in the EDC and in floor and actively collected airborne dust samples of the previously performed EDC validation study. The EDC β-(1,3)-glucan levels correlated moderately with β-(1,3)-glucans in actively collected airborne dust and floor dust samples, while the glucan levels in the airborne dust and floor dust samples did not correlate. The combination of the newly developed β-(1,3)-glucan sandwich EIA with EDC sampling now allows assessment in large-scale population studies of exposure to airborne β-(1,3)-glucans in homes or other low-exposure environments.

Published
2010

225. The protective effect of farm animal exposure on childhood allergy is modified by NPSR1 polymorphisms

Author

Bruce, S, primary, Nyberg, F, additional, Melen, E, additional, James, A, additional, Pulkkinen, V, additional, Orsmark-Pietras, C, additional, Bergstrom, A, additional, Dahlen, B, additional, Wickman, M, additional, von Mutius, E, additional, Doekes, G, additional, Lauener, R, additional, Riedler, J, additional, Eder, W, additional, van Hage, M, additional, Pershagen, G, additional, Scheynius, A, additional, and Kere, J, additional

Published
2008
Full Text
View/download PDF

227. Lung function

Author

Turner, C. J., primary, Kennedy, S. M., additional, Buechte, S. F., additional, Morfeld, P., additional, Bicker, H. J., additional, Lenaerts, H., additional, Kalkowsky, B., additional, Pohlplatz, J., additional, Koesters, A., additional, Piekarski, C., additional, Radon, K., additional, Schulze, A., additional, Ehrenstein, V., additional, van Strien, R. T., additional, Praml, G., additional, Nowak, D., additional, Bonde, J. P., additional, Christensen, S. W., additional, Omland, O., additional, Pronk, A., additional, Preller, L., additional, Doekes, G., additional, Wouters, I., additional, Rooijackers, J., additional, Lammers, J-W., additional, and Heederik, D., additional

Published
2007
Full Text
View/download PDF

231. Beruf — Umwelt — Prävention

Author

Pfefferle, P. I., primary, Kaminski, A., additional, Benz, D., additional, Renz, H., additional, Soost, S., additional, Graupner, I., additional, Morch-Röder, A., additional, Pohrt, U., additional, Worm, M., additional, Bünger, J., additional, Raulf-Heimsoth, M., additional, Sander, I., additional, Brüning, T., additional, Merget, R., additional, Schröder, C. M., additional, Lange, K., additional, Breuer, K., additional, Skudlik, C., additional, John, S., additional, van Kampen, V., additional, Liebers, V., additional, Rabstein, S., additional, Broding, H., additional, Keller, C., additional, Müsken, H., additional, Overlack, A., additional, Schultze-Werninghaus, G., additional, Walusiak, J., additional, Muche-Borowski, C., additional, Schäfer, T., additional, Zahradnik, E., additional, Tolba, R., additional, Renström, A., additional, Doekes, G., additional, Heutelbeck, A., additional, Robert, M., additional, and Hallier, E., additional

Published
2007
Full Text
View/download PDF

232. Symposium 8: Bestimmung von (inhalativen) Expositionen und Belastungen

Author

Kuhlbusch, T. A. J., primary, Raulf-Heimsoth, M., additional, Sander, I., additional, Zahradnik, E., additional, Kespohl, S., additional, Brüning, T., additional, Doekes, G., additional, Noss, I., additional, Wouters, I. M., additional, Thorne, P. S., additional, Brunekreef, B., additional, and Heederik, D. J. J., additional

Published
2007
Full Text
View/download PDF

233. Determinants of house dust, endotoxin, and β-(1→3)- D-glucan in homes of Danish children.

Author

Holst, G., Høst, A., Doekes, G., Meyer, H. W., Madsen, A. M., and Sigsgaard, T.

Subjects
DUST, ENDOTOXINS, BETA-glucans, INDOOR air pollution research, ENZYME-linked immunosorbent assay
Abstract

Little is known about the geographic variation and determinants of bacterial endotoxin and β -(1,3)- d-glucan in Danish house dust. In a population of 317 children, we: (i) described loads and concentrations of floor dust, endotoxin, and β-(1→3)- d-glucan and (ii) their correlations and (iii) assessed their determinants; (iv) Finally, we compared our findings with previous European studies. Bedroom floor dust was analyzed for endotoxin content by the kinetic limulus amoebocyte lysate assay and for β-(1→3)- d-glucan by the inhibition enzyme immunoassay. The parents answered questions regarding potential determinants. We found: geometric means (geometric standard deviations) 186 mg/m2 (4.3) for dust; 5.46 × 103 EU/m2 (8.0) and 31.1 × 103 EU/g (2.6) for endotoxin; and 142 μg/m2 (14.3) and 0.71 × 103 μg/g (7.3) for β-(1→3)- d-glucan. High correlations ( r > 0.75) were found between floor dust and endotoxin and β-(1→3)- d-glucan loads, while endotoxin and β-(1→3)- d-glucan concentrations were moderately correlated ( r = 0.36-0.41) with the dust load. Having a carpet was positively associated with dust load and with endotoxin and β-(1→3)- d-glucan concentrations. Pet keeping, dwelling type, and dwelling location were determinants of endotoxin concentrations. No other determinants were associated with β-(1→3)- d-glucan concentrations. Compared with other European studies, we found lower β-(1→3)- d-glucan loads and concentrations but higher endotoxin loads and concentrations suggesting a geographically determined different composition of Danish floor dust compared with other European regions. [ABSTRACT FROM AUTHOR]

Published
2015
Full Text
View/download PDF

247. Poster

Author

Fiebig, H., primary, Weber, B., additional, Cromwell, O., additional, Jutel, M., additional, Fiedler, G., additional, Hanschmann, H., additional, Hansen, I., additional, Stuck, B. A., additional, Hörmann, K., additional, Klimek, L., additional, Jappe, U., additional, Hoffmann, M., additional, Burow, G., additional, Mühlmeier, G., additional, Maier, H., additional, Mušič, E., additional, Košnik, M., additional, Piller, M., additional, Drachenberg, K. J., additional, Urban, E., additional, Schenn, A., additional, Ruëff, F., additional, Weimer, G., additional, Przybilla, B., additional, Sieber, W., additional, Schoppelrey, V., additional, Pfeifer, M., additional, Steiß, J. O., additional, Lindemann, H., additional, Wolf, H., additional, Schnitker, J., additional, Petermann, F., additional, Bergmann, K. C., additional, Zwacka, G., additional, Steinert, B., additional, Markert, U. R., additional, Bijlsma, P. B., additional, Backhaus, B., additional, Weidenhiller, M., additional, Donhauser, N., additional, Hahn, E. G., additional, Raithel, M., additional, Erkelens, W., additional, Hommes, D., additional, Bruno, M., additional, Akkerdaas, J., additional, van Ree, R., additional, Groot, J. A., additional, Taminiau, J. A. J. M., additional, Meinardi, M. M. H. M., additional, Borowski, C., additional, Schäfer, T., additional, Eberhardt, F., additional, Lepp, U., additional, Becker, W.-M., additional, Zabel, P., additional, Hipler, U.-C., additional, Spoo, J., additional, Bauer, A., additional, Elsner, P., additional, Kuefner, M. A., additional, Schwelberger, H. G., additional, Lange, L., additional, Rietschel, E., additional, Riffelmann, F., additional, Lauter, H., additional, Müller, K.-M., additional, Tränkner, A., additional, Mach, K., additional, Reulbach, U., additional, Geyer, D., additional, Leis, B., additional, Ziegert, M., additional, Ahlert, I., additional, Deichmann, K. A., additional, Heinzmann, A., additional, Allmers, H., additional, Beezhold, D., additional, Hamilton, R. G., additional, Sutherland, E. R., additional, Schwanitz, H. J., additional, Scherer, K., additional, Bircher, A. J., additional, Dymek, S., additional, Lex, C., additional, Balzer, S., additional, Schuster, A., additional, Hülsmeier, L., additional, Barker, M., additional, Müller-Lux, A., additional, Göen, T., additional, Koll, W., additional, Koschel, D., additional, Müller-Wening, D., additional, Kütting, B., additional, Janicke, N., additional, Schippke, D., additional, Langer, C., additional, Schulz, T. G., additional, Turowski, S., additional, Drexler, H., additional, Hallier, E., additional, Bickeböller, H., additional, Heutelbeck, A. R. R., additional, Lässig, W., additional, Nordwig, A., additional, Dellweg, D., additional, Schwarz, H., additional, Goldmann, R., additional, Lorenz, C., additional, Achtzehn, U., additional, Stehle, R., additional, Keiper, B., additional, Jilge, B., additional, Beier, L., additional, Schmidt, E. W., additional, van Kampen, V., additional, Haamann, F., additional, Merget, R., additional, Sander, I., additional, Raulf-Heimsoth, M., additional, Rabstein, S., additional, Brüning, T., additional, Ahrens, T., additional, Muesken, H., additional, Bergmann, K.-Ch., additional, Vetter, M., additional, Heitmann, M., additional, Hunzelmann, N., additional, Schuster, J., additional, Kadar, J., additional, Kespohl, S., additional, Petersen, A., additional, Meyer, H. E., additional, Sickmann, A., additional, Kleber, N., additional, Hinrichs, J., additional, Schocker, F., additional, Becker, W. M., additional, Rozynek, P., additional, Dresselhaus, T., additional, Reuter, B., additional, Henzgen, M., additional, Fahlbusch, B., additional, Rudeschko, O., additional, Schlenvoigt, G., additional, Kroegel, C., additional, Rihs, H.-P., additional, Gaspar, Â., additional, Pires, G., additional, Hohenstein, E., additional, Fiedler, E.-M., additional, v. Pelchrzim, R., additional, Focke, M., additional, Zuberbier, T., additional, Worm, M., additional, Janowska, E., additional, Grycmacher-Łapko, V., additional, Kurek, M., additional, Lippert, U., additional, Niedenführ, S., additional, Fuchs, T., additional, Ludwig, A., additional, Koch, A., additional, Balda, B.-R., additional, Oestmann, E., additional, Philipp, S., additional, Spornraft-Ragaller, P., additional, Hammermann, J., additional, Meurer, M., additional, Ott, H., additional, Wurpts, G., additional, Krieg, R., additional, Al Masaoudi, T., additional, Joussen, S., additional, Kiehl, K., additional, Neis, M., additional, Merk, H. F., additional, Baron, J. M., additional, Schmengler, J., additional, John, S. M., additional, Blaschke, V., additional, Bonnekoh, B., additional, Holzamer, N., additional, Schmidt, U., additional, Ambach, A., additional, Oppermann, H., additional, Thriene, B., additional, Gollnick, H., additional, Kraus, T., additional, Häberle, M., additional, Hoopmann, M., additional, Hehl, O., additional, Werfel, T., additional, Heidrich, S., additional, Kelber, J., additional, Hünecke, P., additional, Kasche, A., additional, Klaus, S., additional, Thiel, M., additional, Buters, J., additional, Weichenmeier, I., additional, Ring, J., additional, Traidl-Hoffmann, C., additional, Behrendt, H., additional, Krämer, U., additional, Lau, S., additional, Kim, S., additional, Mahling, H., additional, Schulz, G., additional, Keil, T., additional, Wahn, U., additional, Mock, B., additional, Kugler, J., additional, Cremer, R., additional, Sandner, B., additional, Kaiser, F., additional, Herbst, R. A., additional, Wahl, R., additional, Suck, R., additional, Kügler, K., additional, Frosch, P. J., additional, Nabe, A., additional, Konturek, P., additional, Simon, K., additional, Kressel, J., additional, Nägel, A., additional, Wilken, V., additional, Strehfeld, T., additional, Neubert, K., additional, Pieper, B., additional, Kuhn, M., additional, Winterkamp, S., additional, Pacurar, A., additional, Senger, D., additional, Beskitas, E., additional, Dorrmann, H., additional, Mueller, M. W., additional, Harwanegg, C., additional, Hiller, R., additional, Kinne, R. W., additional, Schröder, C. M., additional, Mahler, V., additional, Schröder, A., additional, Erdmann, S., additional, Schultis, H. W., additional, Buchwald, F., additional, Hampel, W., additional, Maiss, J., additional, Naegel, A., additional, Zahradnik, E., additional, Doekes, G., additional, Runge, D. M., additional, Schwertner, H., additional, Grize, L., additional, Schindler, C., additional, Surber, Ch., additional, Böckelmann, R., additional, Horn, T., additional, Breithaupt, S., additional, Thiele, J. J., additional, Gutermuth, J., additional, Jakob, T., additional, Heinzelmann, J., additional, Varosi, F., additional, Debevc, F., additional, Pöhlmann, T. G., additional, Seyfarth, L., additional, Kindt, F., additional, Löser, C., additional, Niemeier, V., additional, Gieler, U., additional, Kummer, W., additional, Haberberger, R. V., additional, Klockenbring, T., additional, Stöcker, M., additional, Huhn, M., additional, Bauer, R., additional, Goerlich, R., additional, Fischer, R., additional, Barth, S., additional, Suchodolska, A., additional, Soost, S., additional, Bayerl, C., additional, Ludwig, B., additional, Gancs, P., additional, Häusermann, P., additional, Harr, T., additional, Müller, M., additional, Sachs, B., additional, Riegel, S., additional, Schichler, D., additional, Schrooten, J., additional, Heussen, N., additional, Hilgers, R.-D., additional, Seo, J. W., additional, Franke, I., additional, and Strauss, R., additional

Published
2004
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results