201. Maternal smoking during pregnancy, polymorphic CYP1A1 and GSTM1, and lung-function measures in urban family children
- Author
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Ibrahim Abdulhamid, Xinguang Chen, and Kimberley J. Woodcroft
- Subjects
Male ,Vital capacity ,Urban Population ,Maternal smoking ,Molecular Sequence Data ,Daily smoking ,Biology ,Polymerase Chain Reaction ,Biochemistry ,FEV1/FVC ratio ,Pregnancy ,Cytochrome P-450 CYP1A1 ,medicine ,Humans ,Child ,Cotinine ,Lung function ,Glutathione Transferase ,General Environmental Science ,Base Sequence ,respiratory system ,medicine.disease ,Respiratory Function Tests ,respiratory tract diseases ,Pregnancy Complications ,Maternal Exposure ,Tobacco exposure ,Immunology ,Female ,Gene polymorphism ,Demography - Abstract
Purpose Understanding the interplay between genes and in-utero tobacco exposure in affecting child lung development is of great significance. In this study, we tested the hypothesis that tobacco-related lung-function reduction in children differs by maternal polymorphic genes Cytochrome P450 1A1 (CYP1A1) and Glutathione S-transferase Mu 1 (GSTM1). Materials and methods Data were collected among 370 children (6–10 years old, 81.6% African-Americans) and their biological mothers visiting a large children’s hospital. Study hypotheses were tested using multiple regression method. Results Among the study sample, 143 mothers smoked throughout pregnancy and 72 smoked on a daily basis. Spirometric measures (mean±SD) included were: forced vital capacity (FVC)=1635±431 mL, forced expiratory volume in the first 1 s (FEV1)=1440 ±360 mL, percent FEV1/FVC ratio=89±12, and forced expiratory flow between the 25% and 75% of FVC (FEF25–75)=1745±603 mL. In addition to a tobacco effect on FVC (−131 mL, 95% CI: −245, −17) and FEV1/FVC ratio (42, 95% CI: 1, 83), regression analysis controlling for covariates indicated that for the subsample of children whose mothers were CYP1A1⁎2A homozygous, maternal daily smoking was associated with −734 mL (95% CI: −1206, −262) reductions in FEV1 and −825 mL (95% CI: −909, −795) reductions in FVC; reduced smoking was still associated with −590 mL (95% CI: −629, −551) reductions in FVC. For children of mothers with GSTM1 deletion, persistent daily smoking was associated with −176 mL (95% CI: −305, −47) reductions in FVC. Discussion and conclusions Maternal smoking during pregnancy was significantly associated with lung-function reduction in children, particularly for those whose mothers possessed the polymorphic CYP1A1*2A and GSTM1 deletion.
- Published
- 2011
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