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585 results on '"Cortes, E."'

202. Citrate determination with aconitase immobilized on solid support

Author

Cortes, E., Viniegra, S., Aguilar, M. S., and Juana Gallar

Subjects
Aconitate Hydratase, Spectrophotometry, Swine, Myocardium, Temperature, Animals, Citrates, Enzymes, Immobilized
Abstract

Pig heart aconitase has been immobilized on Enzacryl AA solid support and its kinetic behaviours were studied by using a stirred bath reactor with continuous recycling. In this reactor, the best flow rate has been determined to eliminate diffusional problems. Kinetics constants, thermic stability and pH variations have been compared between the soluble and immobilized aconitase for determination of enzyme-Enzacryl AA effectivity. Stability of the soluble and immobilized aconitase was also studied after repeated use and long-time storage. While the soluble form loses its activity after 24 hr storage, the immobilized form preserves its full activity after repeated usage and long-lasting storage. Finally, an easily measurable parameter has been found to quantitate citrate. The maximum increase of absorbance, is proportional to citrate concentration in a range between 0.2 and 3.2 mM. In conclusion, these results show that the immobilized aconitase system can be used for the determination of citrate with reproductility and great sensitivity. In addition to the simplicity of the assay, great economy in enzyme consumption has been demonstrated, in contrast to the traditional methods of quantitative citrate analysis.

206. Sermoens panegyricos moraes e de mysterios ...

Author

Pereira Cortes e Silva, Joseph Manoel, ed. lit, Ferreira, Pedro, imp, Anonymous, Pereira Cortes e Silva, Joseph Manoel, ed. lit, Ferreira, Pedro, imp, and Anonymous

Abstract

Sign.: [cruz griega]<8>, [parágrafo]<6>, A-Z<8>, 2A-2F<8>, 2G<2>

225. Neumopatía aspirativa iatrogénica

Author

BURDACH, R, primary, MIRKIN, D, additional, VARAS, A, additional, BOZA, L, additional, CORTES, E, additional, BARROS, C, additional, PINO, C, additional, and GRAVET, H, additional

Published
1975
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237. Uses & abuses of jobless pay

Author

De Russy, Cortes E. and Ramel, Gregory J.

Subjects
Unemployment insurance -- Public opinion, Business, Business, general
Published
1981

238. Changes to the dissociation barrier of H2 due to buckling induced by a chemisorbed hydrogen on a doped graphene surface.

Author

Hernández-Hernández, A., Vallejo, E., Martínez-Farías, F., Pelayo, J. Jesus, Hernández-Hernández, L. A., Pescador-Rojas, J. A., Tamayo-Rivera, L., Morales-Peñaloza, A., López-Pérez, P. A., and Cortes, E. Rangel

Subjects
HYDROGEN, DISSOCIATION (Chemistry), GRAPHENE, DENSITY functionals, ELECTRONIC structure
Abstract

An effectiveway of enhancing hydrogen storage on adsorbent materials can be induced by the hydrogen spill-over mechanism, although to date there is no general consensus which satisfactorily explains the mechanism. In this work, a possible reaction path to explain hydrogen adsorption is shown. Density-functional calculations were used to study the dissociation of molecular hydrogen near to a stressed region, as a consequence of chemisorbed hydrogen at the graphene-nitrogen surface. We found that as a result of the buckling induced by the chemisorbed hydrogen, the dissociation barrier of molecular hydrogen diminished by 0.84 eV. The chemisorbed hydrogen is the final state in the spill-over mechanism on a graphene-nitrogen decorated with palladium clusters. This effect helps to create hydrogen nanoislands that may change the diffusion and detrapping of H. An electronic structure analysis suggests that these systems occasionally present metallic or semiconductor behavior.Hydrogen dissociation and adsorption process via buckling defect [ABSTRACT FROM AUTHOR]

Published
2018
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250. 62 Long-Chain Polyunsaturated Fatty Acids in Behavioural Disorders and Neurological Patients.

Author

Carratala, F, Cortes, E, Hidalgo, M J, and Moya, M

Abstract

The omega-3 and omega-6 long-chain polyunsaturated fatty acids (LCPUFA) are crucial to brain development and can be altered, as etiologic explanatory cofactor, both in neurological and psychiatric conditions.Aim: To describe the omega-3 and omega-6 LCPUFA serum pattern in both neurological and psychiatric patients.Patients and Methods: One hundred and seventeen patients were recruited from June 2003 to December 2004. They were categorized into the following groups: Mild Mental Retardation (MR=16); Static Encephalopathy (SE=13); Progressive Encephalopathy (PE=8); Epilepsy (Ep=24); Pervasive disorder (PD=18); Attention Deficit and Hyperactive Disorder (ADHD=20); and a mixed group with recurrent headache, febrile convulsions and soft neurological signs as a Control Group (CG=11). A serum sample was analysed by gas chromatography and mass spectroscopy. Then the total omega-3 and omega-6 LC-PUFA wt/wt percentage was calculated. The Mann-Whitney U test was used to compare every group to CG.Results: 1) Omega-3: The MR group (Median=1.6; CI: 1–4.7) was significantly lower (U=39; p=0.015) than CG (Median=2.3; CI: 1.2–6.9). The SE group (Median=1.7; CI: 1.5–2.3) was significantly lower than CG (U=32; p=0.022). The PE group showed a near significance lower wt/wt % (Median=1.9; CI: 1.4–2.5) (U=23; p=0.091). The Ep, PD and ADHD groups did not show significant differences from CG.2) Omega-6: The MR group (Median=34.3; CI: 25.1–36.3) was near significance lower (U=49; p=0.056) than CG (Median=38.84; CI: 33.60–41.54). The SE group (Median=33.2; CI: 22.9–35.8) was significantly lower than CG (U=30; p=0.015). The PE group was also significantly lower (Median=30.2; CI: 19.6–35.7) (U=12; p=0.007). Again Ep, PD and ADHD groups did not show significant differences from CG.Conclusions: 1) the neurological patients except Ep tend to show omega3 and omega6 LCPUFA lower levels than controls. 2) the behavioural problem patients did not show any differences to the CG.

Published
2005
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