Search

An experiment was carried out to evaluate the effects of replacing corn with by-product from biscuit manufacture (BBM) on feed intake, digestibility, nitrogen balance, ingestive behavior, and growth performance of pure or crossbred Morada Nova sheep. Twenty-four non-castrated male lambs from two genetic groups (GG) were used: purebred red-coated Morada Nova (RMN) and red-coated Morada Nova x white-coated Morada Nova. Each GG had 12 lambs with an initial body weight (BW) of 15.0±0.84 and 18.8±0.88 kg for RMN and crossbred lambs, respectively. Treatments were distributed in a completely randomized design, according to a 2 × 2 factorial arrangement, as follows: two genetic groups (RMN and crossbred) and two diets (ground corn and by-product from biscuit manufacture-BBM) with six replicates. There was no effect (p0.05) of GG on feed intake (g/day and as a fraction of BW). Lambs fed BBM had a lower feed intake (p0.05) than corn-fed lambs, except for the non-fibrous carbohydrate intake, which did not differ among diets (p0.05). No effects (p0.05) of GG or diets were observed on apparent digestibility, except for organic matter, which was higher for BBM-fed lambs (p0.05). Intake and N urinary excretion were lower (p0.05) for BBM-fed lambs when compared to corn-fed lambs. There was no effect (p0.05) of GG on growth performance. On the other hand, lambs fed BBM diet had a higher (p0.05) feed efficiency than corn-fed lambs. Total replacing corn with BBM in finishing lamb diets increases the efficiency of nutrient utilization, which results in higher feed efficiency. The crossbreeding between the red- and white-coated strains of Morada Nova sheep does not affect nutritional and growth performance.

This study was designed to evaluate the seasonal expression of seminal plasma proteins from two bovine breeds adapted to a subtropical climate and their associations with post-thawing sperm and environmental characteristics. Semen samples were obtained three times in summer and three times in winter from four Crioulo Lageano and four Angus bulls. Seminal plasma was obtained by centrifugation, and the other portion of the semen was cryopreserved. Seminal plasma proteins were identified by 2D-nanoUPLC-MSE. Post-thawing assessments of sperm kinetics, morphology and membrane integrity were performed. Environmental data such as air temperature, air humidity and black globe temperature (BGT) were recorded, and the temperature-humidity index (THI) was calculated in summer and winter. Results showed that the climate varied significantly between seasons. Although no statistical differences were observed in semen quality between breeds, the protein profiles varied within and between seasons. We suggest that the most critical proteins in summer affecting sperm characteristics were TIMP-2, DNase, Clusterin, CFAH and GPx6. TIMP-2 and DNase showed a higher abundance in Crioulo Lageano in comparison with Angus, while Clusterin, CFAH and GPx6 presented a lower abundance. To the best of our knowledge, this is the first report of a recently evolved type of glutathione peroxidase, GPx6, in seminal plasma of bovines. In winter, five proteins were considered to be more critical: BSP1, BSP3, CCL2, Sulfhydryl oxidase and TIMP-2. BSP1 and TIMP-2 showed a lower abundance while BSP3, CCL2 and Sulfhydryl oxidase presented a higher abundance in this season in Crioulo Lageano in comparison with Angus.

Lactose has an essential role in milk synthesis, and low lactose content adversely affects dairy product yield. In this study, the association between milk lactose content and other milk constituents (protein and fat), somatic cell count (SCC), and total bacteria count (TBC) was valuated using a database of bulk tank milk (BTM) samples from dairy farms in southern Brazil. Factor and cluster analysis were performed on a dataset containing the fat, protein, and lactose contents, SCC, and TBC of 154,781 BTM samples evaluated by the State Laboratory of Milk Quality (LABLEITE). The factor analysis showed that lactose content in milk was positively associated with fat and protein contents as well as negatively associated with SCC and TBC. The cluster analysis on three clusters showed differences in the fat and protein contents of the BTM samples (P

The aim of this study was to explore spatial genetic structure patterns in cattle breeds adapted to local conditions in Brazil. We georeferenced 876 animals of ten breeds raised in Brazil kept in the Genebank of Brazilian Agricultural Research Corporation (Embrapa) by sample locations using the QGIS 2.4.0 software. The Mantel tests, spatial autocorrelation, and Monmonier tests were performed. The distances for spatial correlation tests ranged from 5 to 15 classes. The results indicated genetic discontinuities in cattle breeds from the Midwest, South, and Southeast of the country. Correlation between genetic and geographic distance was low, but significant. The Monmonier Maximum Distance Algorithm indicated an initial subdivision of Curraleiro and then Pantaneiro from the other breeds. In another subdivision, Criollo, Mocho Nacional, and Caracu were grouped. Genetic discontinuity was observed beyond 431 km, the minimum sampling distance between populations for conservation purposes.

Infrared thermography of the lateral side portion of the udder was taken from 38 lactating cows in a 6-day experiment. Thermograms were analyzed using specific software with the use of eight different methods. The experiment was aimed at correlating the different methods with each other and with rectal temperature (RT), in order to suggest a method to assess udder side temperature, and also at creating regression equations to enable RT calculation through the use of thermographic data. All methods of analyzing thermograms were highly correlated; correlations between thermograms and RT were significant (P

O objetivo deste estudo foi avaliar os aspectos genéticos relacionados à produção e à eficiência reprodutiva de búfalas das raças Murrah, Mediterrâneo e suas cruzas. Uma classificação de touros da Embrapa Amazônia Oriental também foi composta para orientar os cruzamentos assistidos. Foram utilizados 2.322 registros de nascimento de búfalas das raças Murrah, Mediterrâneo e cruzadas do rebanho da Embrapa Amazônia Oriental, de 1953 a 2013, bem como características produtivas e reprodutivas. As análises genéticas foram realizadas pelo software Wombat, utilizando-se o modelo animal com análise de duas características. Enquanto a herdabilidade (h 2 ) para a produção total de leite (PTL) e para a porcentagem de gordura (G) foi alta, para as características reprodutivas a h 2 tendeu a ser baixa. As correlações genéticas da PTL com as demais características foram baixas e negativas, exceto para a PTL com intervalo entre partos (IP) e período de serviço (PS) na raça Mediterrâneo e com idade ao primeiro parto (IPP) e PS nas cruzadas, que foram positivas e altas. O touro 1001 apresentou alta capacidade de transmissão predita (CTP) para a PTL, então deve transmitir um maior volume de leite para seus descendentes, embora com um menor conteúdo transmissível de CTP para G. Portanto, existe variabilidade suficiente dentro do rebanho para trabalhar com o manejo genético para a produção e a eficiência reprodutiva. The objective of this study was to evaluate genetic aspects related to production and reproductive efficiency of Murrah and Mediterranean buffaloes and their crosses. A ranking of bulls from Embrapa Eastern Amazonia was also composed to guide assisted mating. Birth records of 2,322 Murrah, Mediterranean, and crossbred buffaloes from the Embrapa Eastern Amazon herd, from 1953 to 2013, as well as information on production and reproductive traits were used. Genetic analyzes were performed in the WOMBAT software using the animal model with two-trait analysis. While heritability (h2) for total milk production (TMP) and fat milk percentage (F) were generally high, for reproductive traits h2 tended to be low. Genetic correlations for TMP with the other traits were low and negative, except for TMP with calving interval (CI) and service period (SP) in the Mediterranean breed and with age at first calving (AFC) and SP in crossbred, which were positive and high. Bull 1001 had high predicted transmitting ability (PTA) for TMP, so it should transmit a greater volume of milk to his offspring, although it had a lower PTA for F. There was sufficient variability within the herd to work with genetic management for both production and reproductive efficiency.

Textos de Homenagem à professora Sonia Ranincheski, Tribute Texts to Professor Sonia Ranincheski, Textos de homenaje a la profesora Sonia Ranincheski

There are few animal germplasm/gene bank collections in Brazil, and basic studies are needed to attend the future internal and external demands from international partners. The aim of this work was to validate a “proof of concept” that integrates spatial (georeferenced data) and genetic data regarding the local of origin from 3518 DNA samples from 17 different genetic groups or breeds of sheep in the Brazilian Germplasm bank. Spatialisation shows that not all genetic groups have samples in the bank, and collection is concentrated in the conservation nuclei spread nationwide. Only 21% of states with a specific breed have samples in the gene bank. The mean number of animals sampled per collection was 32, while the mean distance travelled to collect samples was 262 km from the conservation nuclei. For example, the Brazilian Somali were only collected in the conservation nucleus in Ceara State. No samples were collected to date for the Cariri breed, which is recognised by the Brazilian Ministry of Agriculture. Only two farms and one breed in the bank are from the northern region. Of the 27 states, there are samples in the gene bank of sheep from 13, so several states have no samples, requiring collection from herds outside the official system of conservation to make sure that studies using this germplasm realised are not biased. Significant genetic differences are seen above 332 km, which should guide future sampling efforts. Suggestions are given for improving the quantity, quality and diversity of samples in the gene bank.

This systematic review and meta-analysis aim to summarize the effects of maternal undernutrition or overnutrition during pregnancy on fetal weight and morphometric measurements during pregnancy, at birth, and postnatal period in sheep. After completing the search, selection, and data extraction steps, the measure of effect was generated by the individual comparison of each indicator with the average of the control and treated group (undernutrition or overnutrition) using the DerSimonian and Laird method for random effects. Subgroup analyses were also performed for lambing order, litter size, sex, as well as level, timing, and duration of the intervention. Fetal weight during the first third of pregnancy was not affected by maternal undernutrition or overnutrition. On the other hand, undernutrition in the second and last third of gestation reduces the weight of the lamb both during pregnancy, at birth, and during the postnatal period, requiring at least 120 postnatal days to achieve the same weight as its contemporaries in the control treatment. However, this reduction in weight is not accompanied by reductions in morphometric measurements, demonstrating that the animals were lighter, but of equal size. In overnutrition, there is an increase in fetal weight in the second third of gestation. However, in the last third of the gestational period, there are no differences in fetal weight for the multiparous subgroup, but it was reduced in primiparous ewes. There are no effects of overnutrition on birth weight; however, this result is highly heterogeneous. Thus, maternal nutrition of ewe during pregnancy has effects on fetal and postnatal weight, but not on size. Furthermore, the effects of undernutrition are more homogeneous while overnutrition showed heterogeneous responses.

Brazil is one of the world’s largest milk producers. Several scientific studies have been developed related to landscape analyses that combine genetic with landscape structure data. In the present study, we aimed to analyze the relationship between genetic, environmental, and socioeconomic aspects of production in Girolando cattle in Brazil, as well as verify the spatial patterns of its genetic diversity. Genetic values and accuracy of 46,289 animals were used as well as information from DNA of 310 Girolando animals. Canonic, discriminant, and cluster analyses were conducted in SAS® and K-means method in ArcGIS 10.3 software. The relationship between genetic and geographic distance was analyzed using different methods in software Alleles in Space®. Clusters with animals with higher genetic values for milk production are located in municipalities with lower gross domestic product, fewer family-based establishments, and lower human development index. These clusters are associated with regions with higher area planted with crops, lower percentage of pastures that were less degraded, higher humidity, lower temperature range, and lower normalized difference vegetation index (NDVI) values. The greater the geographical distance between groups of animals, the greater the genetic distance between them with a significant distinction over 504 km. There is high genetic heterogeneity among animals. From these results, it will be possible to develop methodologies for better evaluation of the animals within the production systems.

Background Reproductive capacity can be altered by challenges experienced during critical periods of development, including fetal development and early neonatal life. Gossypol is a polyphenolic compound, commonly found in cotton seeds, that impairs male reproduction. Here, we investigated whether the exposure to gossypol in utero and during lactation alters male reproductive function in sheep. From conception until 60 days postpartum, ewes were randomly assigned to a control diet or a gossypol-rich diet based on cottonseed. Lamb testicles were removed at 60 days of age and subjected to RNA-sequencing. Results Lambs derived from the maternal cottonseed diet showed significantly lower growth and lower testis weight as a proportion of the total body weight, and reduced testosterone levels. In addition, the testis transcriptome was significantly altered by the maternal cottonseed diet. Most of the altered genes are directly implicated in testis development and sperm biology, cell communication, iron ion metabolism, calcium homeostasis and signaling, among other functions. Interestingly, network analysis revealed that exposure to gossypol significantly disturbed coexpression patterns among spermatogenesis-related genes, suggesting a disruption in coregulation mechanisms. Conclusions Our findings provide evidence that maternal exposure to gossypol alters male reproductive function in the offspring, with potential lasting or lifelong negative consequences.

The use of new feed resources, particularly local agroindustrial byproducts, such as banana, may be an option for replacing those traditionally used for sheep feed to reduce production costs. Thus, the aim of this study was to evaluate the dietary effects of replacing corn bran with banana leftovers on performance, carcass, non-carcass components, meat traits, and fatty acid profile of Santa Inês lambs. Twenty-four Santa Inês female lambs with an average weight of 23.73 kg were fed diets containing 60 % coast cross hay and 40 % concentrate (30 % corn bran and 10 % soybean meal). Treatments consisted of corn bran replaced by banana leftovers at rates of 0, 25, 50, and 75 % on a dry matter basis. The experiment lasted 120 days. Animals were slaughtered and carcasses and non-carcass components were evaluated. The half-carcasses were weighed and sectioned into commercial cuts. The 12th and 13th ribs were dissected to collect bones, muscle and fat proportions. Cooking loss, color, shear force and sarcomere length were measured. Fatty acid profiles were obtained by gas chromatography. Hot and cold carcass weight, leg, neck, lung, loin eye area, fat thickness, initial sample weight of the 12th and 13th ribs, fat and bone, presented a negative linear effect of banana leftovers replacing corn in the diet. Loin, fat thickness, cooking loss and carcass redness showed a negative quadratic effect, while full and empty abomasum, full omasum, sarcomere length and yellowness presented a positive quadratic effect. Replacement of up to 75 % of corn bran by banana leftovers did not interfere in the intake, performance, meat traits and the fatty acid profile of lambs. The use of banana leftovers may be an alternative for reducing animal production costs.

Context Using legumes in ruminant production system may provide benefits such as improvement in animal performance and decrease in methane (CH4) emissions, mainly based on nutritional characteristics of these plants and the presence of tannins. Macrotiloma (Macrotyloma axillare) is a tropical legume that still lacks studies regarding its effects on animal performance and CH4 emissions. Aims The objective of this experiment was to evaluate productive performance, CH4 emission, ruminal fermentation parameters, carcass characteristics and fatty acid profile of the meat from lambs fed tropical grass hay supplemented with the legume forage macrotiloma. Methods For a 90-day experimental period, 14 Santa Inês male lambs (aging 100 days; 18.06 ± 3.26 kg initial bodyweight) were allocated in individual pens and divided into two treatments: chopped aruana grass (Panicum maximum cv. Aruana) hay (CON); aruana hay supplemented with chopped macrotiloma hay at a ratio of 75:25 grass to legume (MAC). Lambs were weighed fortnightly for calculation of average daily bodyweight gain (ADG) and CH4 emission was measured on three occasions (days 28 to 32; 57 to 61; 85 to 89) using the sulfur hexafluoride (SF6) tracer technique. At the last day of CH4 collection, rumen fluid samples were collected for: determination of ammoniacal nitrogen (NH3-N) and short chain fatty acids (SCFA); ruminal protozoa count; and relative abundance determination of general bacteria (BACT); Ruminococcus flavefaciens (RUMI); Fibrobacter succinogenes (FIBRO); methanogenic archaea (METH) by real-time quantitative PCR (real-time-qPCR) analysis. Subsequently, after 16 h fasting, the animals were slaughtered; carcass characteristics were then evaluated and longissimus thoracis samples were collected for fatty acid profile analysis. Key results No significant difference (P > 0.05) was observed between MAC and CON for productive performance, CH4 emission, SCFA and carcass characteristics. Reduced protozoa count and relative abundance of METH were observed for MAC (P < 0.05). Conclusions Despite the lack of effects on productive performance and CH4 emission, the decreased relative abundance of METH and protozoa count indicated that macrotiloma may possibly present anti-methanogenic activity. Implications The inclusion of this legume in the diet of ruminants may affect rumen microbes and potentially decrease environmental impacts of the production system.

The trade in live animals between India and Brazil dates from the late nineteenth century when European travellers traded animals of Indian origin for display in zoos. Considering the origin of coffee and sugar cane, as well as the expertise related to mineral evaluation, we need to consider that India was involved in important economic cycles of Brazil, even indirectly. This virtuous flow of trade has been maintained and intensified throughout modern history, especially after these two nations gained political independence from their colonisers, thereby becoming independent in mercantile affairs. This paper addresses the main points related to the use of animals of Indian origin in Brazil. We revisit some of the historical aspects of the process of colonisation of Brazil, as well as the importation of animals from India. The restrictions imposed on this process due to the occurrence of diseases in cattle and buffalo in India will be examined. At the end of the text, emphasis will be given to the risks of introducing exotic diseases into Brazil.

O objetivo do presente estudo foi avaliar medidas morfométricas de bovinos da raça Crioula Lageana em diferentes idades, para estabelecer padrões de variação dessas medidas, contribuindo para a caracterização fenotípica da raça. Foram coletadas medidas corporais (comprimento corporal, altura da cernelha, perímetro torácico, altura da garupa, largura da garupa e comprimento de garupa) de 346 animais oriundos de três regiões do Planalto Catarinense (Campos de Curitibanos, Coxilha Rica e Campos de Caçador). As medidas morfométricas foram avaliadas em relação à idade, sexo e região de origem. A análise estatística foi realizada utilizando SAS versão 9.3. Os procedimentos utilizados incluíram análise de variância, correlações, cluster, discriminante e análise canônica. Foi observada diferença estatística entre machos e fêmeas para todas as medições, exceto altura da garupa, demonstrando o dimorfismo sexual. A altura da garupa variou até 3 e 5 anos de idade e comprimento do corpo variou em animais com mais de 11 anos de idade; enquanto a largura da garupa e perímetro torácico variaram até 7 e 9 anos de idade. Em média, 50% dos animais foram classificados correctamente, o que indica pouca diferença entre as regiões para as características morfométricas. Além disso, animais de Campos de Curitibanos foram os que apresentaram maior distância dos animais das outras regiões do planalto de Santa Catarina. As correlações entre altura da cernelha, largura e comprimento da garupa foram acima de 0,80. Assim, o comprimento da garupa pode ser considerado a medida mais adequada para avaliação do tamanho corporal desta raça, devido à alta correlação com as outras medidas e a facilidade de medição deste parâmetro.

Traditional livestock productivity is hard to estimate, since it depends on a gamut of animal production indicators that are difficult to measure for many farms. Thus, we propose an analytical method for estimating productivity and understanding the importance of animal production indicators under different full-cycle cattle production systems in Brazil. To evaluate the impact of these indicators, equations were derived from a comutational model of herd evolution for estimating the output parameters of the system as follows: productivity per hectare (PH) and offtake rate (COR), as a function of the indicators; calving rate (CR), mating age (AM), age of slaughter (AS) and stocking rate (SR). For this analysis, twenty-seven scenarios (simulation data) of low to high productivity were used, resulting from the combination of the following factors and levels: 1) calving rate of 50, 65 and 80 %; 2) mating age of heifers of one, two and three, years of age; and 3) age at slaughter of one, two and three year old steers. The scenario with the highest impact for each parameter and the indicator of highest impact for each scenario were identified for the production conditions in the region. Under most scenarios, a reduction in mating age had a greater impact on the productivity indexes compared to a reduction in slaughter age. Appropriate management of available technologies enables farmers to compare the marginal impacts of specific indicators on full-cycle production systems for beef cattle.

Pre-loading handling and conditions of transport are related to welfare, disease risk and product quality of production animals. These steps continue to be one of the major animal management problems in Brazil. This study evaluated the effects of different types of pre-loading handling and road transport times on the haematological and biochemical traits of cattle. Eighteen male cattle were submitted to three travel times (24, 48 and 72 h) in a truck soon after load using different types of pre-loading handling: traditional (rough handling), training (gentle handling) and use of flags to movement cattle. Haematological traits, blood biochemical measures as well as blood and faecal cortisol were analysed in order to assess animal welfare and physiological status. The traditional management showed to be more stressful, also had animals with a greater number of neutrophils and lower numbers of lymphocytes than handling with flags, showing that animals submitted to more stressful situations can have compromised immune system. Serum aspartate aminotransferase concentrations were within the reference levels and when taken together with increased creatine kinase patterns observed indicate muscle damage in traditional management. Decrease in glucose concentrations over time from traditional management to flag management was observed, while fructosamine was increased in traditional management with 72 h of travel. When taken together, all reported factors, immune, enzymatic, energetic and hormonal, indicate that the quality of pre-loading handling and time of transport were determinant for animal welfare, its homeostatic balance and sanitary conditions. Keywords: Animal welfare, Blood cortisol, Faecal cortisol, Haematological evaluation, Road transport

Background: The lack of information on population structure is one of the main obstacles to develop breeding and conservation programs for animal genetic resources. Objective: To characterize the population structure of Crioula Lageana cattle breed (Bos taurus) in order to assess its genetic diversity. Methods: Database with information of 1,638 Crioula Lageana animals, collected during 38 years, was analysed using the ENDOG v.4.4 program. Results: Effective population size ranged from 72.53 in complete generations to 143.90 in maximum generations. Inbreeding and Average Relatedness coefficients were 0.34 and 0.91%, respectively. The effective number of founders and ancestors were 29 and 28 animals, respectively, and only ten ancestors were responsible for 50% of the genetic variability of the whole population. The average generation interval was 5.84 years in the paternal line and 7.70 in the maternal one. Wright´s F statistics indicated low genetic distances between subsets in relation to the total population (Fst = 0.0015), between individuals with respect to their subpopulation (Fis = -0.0027), and between individuals in relation to the total population (Fit = -0.0012). Conclusion: Analysis of the population indicated that, despite the small number of animals with known parentage and considerable loss of genetic variability by the constant use of a few sires, and same value of number of founders and ancestors, the population showed good genetic management, low inbreeding, low genetic differentiation among subpopulations, and probably adequate effective population size for breed preservation. Keywords: conservation of animal genetic resources, genetic diversity, inbreeding, locally-adapted breed, pedigree analysis. Resumen Antecedentes: La falta de informacion sobre estructura poblacional es una de las principales barreras para el desarrollo de programas de mejoramiento genetico y conservacion de los recursos zoogeneticos. Objetivo: Caracterizar la estructura poblacional de la raza bovina Crioula Lageana ( Bos taurus ) para evaluar su diversidad genetica. Metodos: Una base de datos con informacion de 1.638 animales Crioula Lageana ( Bos taurus ), recogidos durante 38 anos, fue analizada utilizando el programa ENDOG v.4.4. Resultados: El tamano efectivo de la poblacion vario de 72,53 en las generaciones completas a 143,90 en las generaciones maximas. La endogamia y la relacion media de los coeficientes fue 0,34 y 0,91%, respectivamente. El numero efectivo de fundadores y antepasados fue de 29 y 28 animales respectivamente, y solo diez antepasados fueron responsables del 50% de la variabilidad genetica de la poblacion. El intervalo promedio de generacion fue de 5,84 anos en la linea paterna y de 7,70 en la linea materna. El indice estadistico de Wright’s F indica una baja distancia genetica entre los subconjuntos en relacion con la poblacion total (Fst = 0,0015), entre los individuos con respecto a su subpoblacion (Fis = -0,0027), y entre los individuos en relacion con la poblacion total (Fit = -0,0012). Conclusion: El analisis de la poblacion indica que a pesar del pequeno numero de animals con origen conocido y la considerable perdida de variabilidad genetica por el uso constante de pocos toros y el mismo valor del numero de fundadores y antepasados, la poblacion mostro un buen manejo genetico, baja endogamia, baja diferenciacion genetica entre las subpoblaciones y probablemente un tamano efectivo adecuado de la poblacion. Palabras clave: analisis de pedigri, conservacion de los recursos geneticos animales, diversidad genetica, endogamia, razas adaptadas localmente. Resumo Antecedentes: A falta de informacoes sobre a estrutura da populacao esta entre os principais obstaculos ao desenvolvimento de programas de melhoramento e conservacao de recursos geneticos animais. Objetivo: Caracterizar a estrutura populacional da raca bovina Crioula Lageana ( Bos taurus ) para acessar a diversidade genetica da raca. Metodos: Banco de dados com informacao de 1.638 animais Crioula Lageana, recolhidos durante 38 anos, foi analisado utilizando EndoG v.4.4. Resultados: O tamanho efetivo populacional various de 72,53 nas geracoes completas para 143,90 nas geracoes maximas. Coeficientes de Endogamia e Relacao foram 0,34 e 0,91%, respectivamente. O numero efetivo de fundadores e ancestrais foram 29 e 28 animais, respectivamente, sendo que apenas dez ancestrais foram responsaveis por 50% da variabilidade genetica de toda a populacao. O intervalo de geracoes foi de 5,84 anos para linha paterna e 7,70 para linha materna. As estatisticas F de Wright indicaram uma pequena distância genetica das subpopulacoes entre os subgrupos em relacao a populacao total (FST = 0,0015), entre os individuos em relacao a sua subpopulacao (FIS = -0,0027) e entre individuos em relacao a populacao total (Fit = -0,0012). Isto indica uma baixa diferenciacao genetica na populacao estudada. Conclusao: A analise populacional indicou que, apesar do numero pequeno de animais com ascendencia conhecida e consideravel perda de variabilidade genetica pelo uso constante de alguns touros e mesmo valor do numero de fundadores e antepassados, a populacao mostrou boa gestao genetica, endogamia baixa, baixa diferenciacao genetica entre subpopulacoes e provavelmente adequado tamanho efetivo da populacao para a preservacao da raca. Palavras-chave: analise de pedigree, conservacao de recursos geneticos animais, diversidade genetica, endogamia, racas localmente adaptadas.

A national milk productivity index was defined and then used to direct the suitability of best management practices programs using milk production (i.e., milk production, value of milk and cow numbers by municipality, productivity per cow per municipality and value of milk per cow) as well as socioeconomic and environmental data for Brazilian municipalities. Production data were analyzed using SAS®v.9.3, generating clusters (high, medium, low and no milk productivity) which were then subjected to spatial analysis using ArcGIS 10 software. Socioeconomic and environmental characteristics of production clusters were also statistically discriminated using SAS®v.9.3. Eleven general characteristics of six best management programs available to the Brazilian farmer were classified by experts to ascertain where they should be applied and where government should focus its resources. The six programs studied included: Safe food Program (SFP); Good Practices for Sustainability Program (GPSP); Good Practices on the Farm Program (GPFP); Quality Milk Production Program (QMPP); Educampo Project (EP); and Full Bucket Project (FBP). The eleven general characteristics of these programs included: ease of implementation, time required for implementation, cost of implementation and monthly maintenance, evaluation by milk producers, evaluation by dairy industries, stimulation of additional payment for milk quality, adhesion to best management practices of milk and dairy products, producer-dairy integration, practical application and target audience priority, and adaptability to the FAO & IDF Guide (2013). SFP is more suitable for farms with higher productivity due to difficult implementation, medium cost of monthly maintenance, in municipalities with a high milk productivity index. QMPP could be used to improve milk quality, especially in small and medium dairy farms in locations with low or medium milk productivity indices.

We evaluated Haemonchus contortus (HC) and Trichostrongylus colubriformis (TC) infection on the ruminal microbial community of Santa Ines lambs to better understand the pathophysiology of parasite infections and the interactions among gastrointestinal nematodes and gut resident microbiota. In this study, 18 six months of age lambs were maintained for 34 days in individual pens divided into three treatments that included animals infected with HC and TC, and control (infection-free). Haematological, ruminal parameter and microbial nitrogen absorbed by pune derivatives, as well as enteric methane emission (CH4), were analysed, and the rumen microbial taxonomic and functional profile assessed by shotgun metagenomics. The analysis showed that total protein, albumin, urea, and butyrate level were lower in animals infected by both parasites, while HC infection also decreased the haemoglobin level. Both infected groups (TC and HC) increased the enteric methane emission (CH4). TC and HC infections increased the diversity and richness of functional microbial genes. Most alterations in the rumen microbiome composition of infected groups are associated with the suppression of microbes involved in microbial homeostasis maintenance and expansion of the archaeal community in the infected animals. Infection led to an increased abundance of nitrogen, amino acid, protein, and energy metabolism genes. Overall, TC and HC infection increased the enteric methane emission, negatively affected taxon's responsible for maintenance de rumen homeostasis and modulate some important genes related to protein and energy metabolism.

As gene bank collections develop, there are opportunities to collect germplasm using criteria other than random sampling or genetic relationships. For Brazil and the US, it has become apparent that sampling based upon environmental adaptability could enrich germplasm collections. Combining current US beef cattle germplasm collection with Geographical Information System (GIS) approaches, sample concentration and underrepresented areas were identified for Bos taurus taurus and Bos taurus indicus (BI). BI samples were principally derived along the Gulf Coast region (not necessarily close to freshwater sources). In contrast, continental Europe (BTCE) and British, African and Asian (BAA) breed samples were more concentrated among the Great Plains/Mid-West (close to major rivers and lakes), with some samples being derived from the south-west and Gulf Coast areas. BTCE and BAA have been extensively sampled, but more is needed in the more westerly states of New Mexico, Nevada, California and Utah. In addition, BI sampling should increase in the more northerly states of Tennessee and Kentucky to capture allele frequencies that may better enable those breeds to perform outside the Deep South. The methods used in this evaluation can be extended to using genotypes within or among breeds to promote sampling in environmentally unique areas.

This aim of this study was to analyze the influence of socieoconomic, environmental and farm variables on the existing genetic values (Expected Progeny Differences - EPDs) of the different clusters of Nelore animals in Brazil. The data evaluated included EPDs of 1,462,485 animals, males and females born between 2000 to 2011, same period of the compiled variables used (socieoconomic, environmental and farm), distributed in 171 municipality from 15 Brazilian states by GENEPLUS (Genotype Breeding Program of Embrapa Beef Cattle). The municipality averages of sixteen variables were used for weight traits (maternal and direct genetic effects for weight at 120 days, maternal and direct genetic effects at birth and weaning weight, post-yearling weight and average daily gain post-weaning), carcass quality (carcass conformation at weaning, carcass conformation at post-yearling, eye muscle area, subcutaneous fat thickness and marbling) and reproductive traits (scrotal perimeter at weaning, scrotal perimeter at post-yearling, age at first calving and cow weight at weaning) as well as their respective accuracies. The data were spatialized in the ArcGIS program along with environmental factors and human development indicators. The statistics included clustering, discriminant and canonical analyzes using the SAS® program. Three clusters for weight traits, five for carcass quality, four for reproductive traits and five for accuracy were formed. The clusters showed good distribution across the country, highlighting the clusters with highest genetic values for each trait type. Discrimination was seen among environments for beef cattle at pasture, depending on climatic conditions. The spatial distribution of the Nelore cattle breed may provide a better understanding of the real situation of the breed in different Brazilian climates and environments. The results of this study can be used to correct possible errors of genetic selection programs that use the same breeders and females for different environments.

Weaning calves at a young age can generate great stress, but it is widely practiced in the industry despite that. So, to what extend weaning in early ages is more stressful than at later ones, in terms of the amount of stress, is currently unknown. Thus, we studied the behavioural and physiological impacts in beef calves at three ages at weaning (30, 75, and 180 days) and the consequences of the calf's sex. Thirty-six calves were weaned and distributed in hyper-early (W30), early (W75), and conventional (W180) weaning groups, which are weaning ages that occur in practice. Behavioural activities and physiological parameters were measured on days 0, 1, 2, and 7 after weaning. Canonical component analysis (CCA) and analysis of variance (ANOVA) were used to evaluate behavioural and physiological parameters. The variables analysed were influenced by the treatment, day of evaluation and interaction between treatment × day, and without effect for sex. The younger the calf, the greater the intensity of responses to the weaning stress. In this sense, the behavioural results (percentages of observations ± SEM) demonstrate that on D0, W30 calves emitted more extreme vocalization (61 %) compared to W75 (15 %) and W180 (0%) (P

O objetivo do presente trabalho foi realizar uma análise de viabilidade populacional da raça bovina Crioula Lageana, para estudar seus parâmetros demográficos e genéticos, bem como verificar a interferência de fatores que podem afetar a sobrevivência eo desenvolvimento dessa população. A viabilidade da população foi estimada utilizando 128 cenários hipotéticos para avaliar o risco de extinção deste recurso genético, simulada por um período de 500 anos. Os resultados mostraram que a possibilidade de extinção desta raça durante os próximos 500 anos foi remota, mesmo em cenários menos favoráveis. A taxa de crescimento determinística (det.r) foi sempre positiva durante o período avaliado, sendo a mortalidade dos animais de até um ano de idade o fator mais importante que afetou este parâmetro. O tamanho da população aumentou rapidamente até atingir a capacidade de suporte da área (k), sendo este o principal fator limitante para o crescimento da população. O nível de heterozigose esperada (He) da população no pior cenário foi superior a 80% e o coeficiente de endogamia (F) foi inferior a 0,20 no final dos 500 anos de simulação. A análise da viabilidade populacional mostrou que a população de Crioula Lageana não apresentou risco aparente de extinção a longo prazo e que a capacidade de suporte da área é o principal fator limitante para o crescimento da população.

This study consisted of two experiments with the following objectives: to evaluate the effects of tannins from the tropical legume macrotiloma (Macrotyloma axillare) on total gas and methane (CH4 ) production, as well as on ruminal fermentation parameters by performing an in vitro bioassay, with samples incubated with and without polyethylene glycol (PEG) in a semi-automatic system; and secondly in a 17 day in vivo experiment, to determine apparent total tract digestibility (ATTD) of dietary nutrients and ruminal fermentation parameters of 12 intact 8- to 9-month-old Santa Ines (averaging 24.95 ± 1.8 kg body weight) ewes fed tropical grass hay supplemented with macrotiloma hay. The ewes were divided into two treatment groups depending on their diet: chopped aruana grass hay (Panicum maximum cv. Aruana) (control-CON); and aruana grass hay supplemented with chopped macrotiloma hay (macrotiloma-MAC). The animals were kept for 5 consecutive days in metabolic cages for the ATTD assay, and at the end of this period, samples of rumen fluid were collected from each ewe to determine ammoniacal nitrogen (NH3 -N) and short-chain fatty acid (SCFA) production, and protozoa count. For the in vitro assay, a decrease in total gas and CH4 production was observed for samples incubated without PEG (p .05). In the in vivo experiment, increased intake and ATTD of crude protein were observed for the animals fed MAC when compared to CON (p

Strategies for the sustainable intensification of ruminant production suggest breeding animals adapted to the environment in which they live using local fodders to maximize efficient ruminal fermentation. The purpose of this study was to explore the effects of using Orbignya phalerata (Babassu) and Combretum leprosum (Mofumbo) leaves in ruminal short chain fatty acids (SCFA) and microbial community, as well as finishing performance, carcass characteristics and meat fatty acid profile of Santa Ines hair lambs. The experimental treatments were diets with 50:50 forage:concentrate ratios, using the leaves of the experimental plants as a 33 g/100 g of dry matter (DM) replacement of Cynodon dactylon (Tifton-85) hay, with three groups: Control (no hay replacement), Babassu and Mofumbo. Twenty-four Santa Ines lambs (body weight = 20.0 ± 5.2 kg) were used in a randomized experimental design with eight repetitions (5 males and 3 female) per treatment. Ruminal fluid samples of each animal were collected to determine SCFA and microbial community. The male animals were evaluated for finishing performance and carcass characteristics while longissimus lumborum muscle samples were used for determination of fatty acid profile. Interaction between treatment and sex was observed for total SCFA ( P P Ruminococcus flavefaciens , and lower Archaea. There was no difference between treatments ( P > 0.05) for finishing performance and carcass characteristics but meat fatty acid characteristics were affected by treatments. A redundancy analysis based on microbial abundance profile demonstrated two clusters, one cluster with Babassu and Control treatments, and other cluster with Mofumbo, with some variables associated to dissimilarity between clusters. These results indicated that the inclusion of these plants in lamb diets affects ruminal short chain fatty acids and microbial population, without compromising the production potential, carcass characteristics and meat fatty acid profile.

The study of locally adapted breeds has the potential to underpin the discovery of genes involved in economically and ecologically important traits. Brazilian locally adapted sheep breeds have distinctive characteristics that could be of value for specialized production systems. Therefore, the main objective of the present study was to identify genomic regions that may have been under selection and therefore may explain ecological and production differences observed among three important Brazilian locally adapted sheep breeds. Animals from the Brazilian Creole, Morada Nova and Santa Ines breeds were genotyped using the Illumina Ovine SNP50 BeadChip. The identification of selection signatures was based on two groups of methodologies: differentiation among populations (FST) and linkage disequilibrium (iHS and RsB). Taken together, these analyses allowed for the identification of 86 candidate genes. Functional analysis revealed genes related to immunity, nervous system development, reproduction and sensory perception. A number of genes are of particular interest including: RXFP2, which has recently been associated with the presence/absence and morphology of horns in sheep; the TRPM8 gene, involved in regulation of body temperature at low temperatures; DIS3L2, PLAG1 and NIPBL, associated with height variation; and finally, SPEF2 and SPAG6, important for spermatogenesis. Selective sweeps were identified using multiple methods, and in a number of cases sweep regions contained genes with a demonstrated role in phenotypic variation. The genomic distribution of the sweep regions differed between populations, suggesting that breed specific signatures were successfully identified that may reflect the consequence of local adaptation.

A intensificação da produção de tilápias pode gerar implicações negativas sobre o desempenho e o bem-estar dos peixes. Assim, é essencial determinar a densidade correta para otimizar a produção. O objetivo deste estudo foi avaliar o desempenho e o bem-estar de tilápias-do-nilo (Oreochromis niloticus) juvenis, com peso inicial médio de 30g (± 2,70), criadas em três diferentes densidades de estocagem, em gaiolas de flutuação. Os peixes foram alimentados três vezes/dia, com ração contendo 32% de proteína bruta, durante 74 dias. O desenho experimental foi completamente ao acaso, com três tratamentos (250 peixes/m3, 350 peixes/m3 e 450 peixes/m3) e quatro réplicas. Os parâmetros físico-químicos da água foram monitorados durante o experimento. A elevação da densidade de criação causou redução no peso final dos peixes, no ganho de peso, no ganho de peso diário, no comprimento padrão e na taxa de sobrevivência, bem como elevou a taxa de conversão alimentar. Entretanto, densidades mais elevadas reduziram o efeito na variação de peso. Não houve influência da densidade de criação nos parâmetros de biomassa final, da concentração de glicose sanguínea e de cortisol sérico. Portanto, o aumento na densidade de criação compromete o desenvolvimento e a taxa de sobrevivência dos peixes, mas não influencia os parâmetros fisiológicos de estresse. Assim, o tratamento com 250 peixes/m3 apresentou resultados mais apropriados ao melhor desempenho dos peixes. Intensification of tilapia production can cause negative implications on performance and welfare of fishes. Thus, it is essential to determine a correct density for optimal production. This study aimed to evaluate performance and animal welfare of juvenile Nile tilapia with an initial weight average of 30g (± 2.70) reared using three different stocking densities in floating net-cages. Fish were fed three times a day with feed containing 32% crude protein during 74 days. The experimental design was completely randomized with 3 treatments (250 fish/m3, 350 fish/m3 and 450 fish/m3) and 4 replicas. The physical - chemical parameters of water were monitored throughout the experiment. Increase in stocking density caused a decrease in the final weight of fish, weight gain, daily weight gain, standard length and survival, as well as an increase in feed conversion. However, higher densities seem to reduce the effect on weight variation. There was no influence of stocking density on final biomass, blood glucose and serum cortisol concentrations. Therefore, the increase of stocking density compromised development and survival of fish, but did not influence the physiological parameters of stress. Thus, treatment with 250 fish/m3 was shown to be the most suitable for fish performance.

The aim in this paper was to evaluate the spatial distribution of protected areas defined by law and their importance as structural corridors. The study area was 7,559,783.69 ha located in Western Bahia (Northeast Brazil), restricted to the limits of the Urucuia Group (Upper Cretaceous), where there is strong agribusiness growth. Currently, a major dilemma in Brazilian public policy arises from the conflicting interests of environmental conservation and increased food production. Brazilian environmental protection policies include the implantation of Protected Areas (Full Protection Units and Sustainable Use Units) and the adoption of the National Forest Act (Permanent Preservation Areas and Legal Reserves). In this context, we delimited illegal land-use in Permanent Preservation Areas (PPAs) adopting the intersection between land-use/land-cover data from PRISM/ALOS image classification for the years 2007–2010 and PPA vectors. We performed the temporal analysis in PAs considering land-use/land-cover data from Landsat TM image classification for the years 1988, 1992, 1996, 2000, 2004, 2008 and 2011. Finally, we performed a Morphological Spatial Pattern Analysis (MSPA) to evaluate whether PPAs alone are sufficient as structural corridors. Hypothetical scenarios were simulated to increase the potential of PPAs as structural corridors. The calculation of MSPA attributes was conducted considering 3 edge widths: 15 m (1 pixel), 510 m (34 pixels), and 1005 m (67 pixels). Four scenarios were simulated, considering a gradual increase in preserved areas. The results show that illegal land use is contained within PPA and protected areas. The scenario simulations present alternatives to increase the connectivity of the fragments and ensure the maintenance of ecological and hydrological services. Rapid agricultural expansion without proper landscape planning can compromise the sustainability of ecosystem services and the recharge zone of the Urucuia aquifer.

For the past 20 years countries have initiated programs to sustainably conserve farm animal genetic resources. At the same time the growing need for increased animal productivity has emerged. Viewing gene banks and in vivo conservation in the context of food security, climate change, and product demand suggests the need for a more efficient use of these mechanisms to support sustainable productivity. Some advances have been made in developing and implementing in-vivo conservation programs, but those efforts appear to be predicated upon various types of government subsidies, which are subject, to policy changes. Given the in-vivo situation, it is suggested conservation efforts shift toward gene banks as the primary conservation mechanism. Globally, national gene banking efforts have increased and they have the capacity and potential to become more dynamic, incorporate different biological materials and facilitate increased use of genetic diversity. The next steps for gene banks are to better utilize information systems to integrate and store data from genetic/genomic assessments, cryopreservation, phenotypes and environmental conditions. These types of benefits plus the reduced conservation costs gene banks can speed the rate of conserving breeds while freeing the livestock sector to increase productivity with the breeds of their choosing.

Respiration rate and eye temperature were collected on 80 sheep from 11 breeds (Australian Merino, Polwarth/Ideal, Corriedale, Romney Marsh, Crioula, Hampshire Down, Texel, Ile de France, Suffolk, Santa Ines and Dorper) during 5 days, giving a total of 1071 observations. All sheep were adult and non-lactating. Data were analysed using SAS® procedure NLIN, using broken line regression to determine temperature humidity index (THI), air temperature and relative humidity limits by breed for respiration rate and eye temperature. These inflexion points were then used to determine regions suitable for sheep rearing by breed, using mean THI by municipality which were then plotted using ARCGIS v.9.3. Clear boundaries were found for sheep breeds, with wool breeds mainly limited to the south and southeast as well as coastal areas. Not all breeds are indicated to be reared in all areas of the country and some regions are unsuitable for any breed. Breed adaptation is in line with their natural history of territorially occupation.

Brazilian hair sheep constitute a genetic diversity hotspot of sheep breeds. These locally adapted genetic resources developed in harsh environments of the Brazilian Northwest (semi-arid) and maintained important traits for this region, such as parasite resistance, heat tolerance and high pelt quality. Genotypes (50K SNP chip) from seven Brazilian sheep breeds (5 hair and 2 coarse wool types) and 87 worldwide breeds were used to verify population structure, admixture and genetic diversity, using PCA and ADMIXTURE analyses. We constructed a phylogenetic tree and evaluated migration events between genetic groups using TREEMIX software. Brazilian Somali, a fat-tailed breed, was the unique breed with high relationship with East African breeds and formed a distinct cluster from other Brazilian breeds. This breed seems to contribute to formation of Santa Inês, Morada Nova and Brazilian Fat-tail breeds. Brazilian Blackbelly had a clear relationship with Barbados Blackbelly, which appeared as another group. Other Brazilian breeds seem to form a further genetic group with some recent admixtures. Morada Nova remained as a separate group, not showing a strong relationship with European or African breeds, only revealing a migration event from Sidaoun, an Algerian hair breed. Brazilian Fat-tail and Morada Nova share a common ancestor, but the first received introgressions from Brazilian Somali and Afrikaner breeds, explaining the fat-tail phenotype. Santa Inês received strong contribution from Bergamasca and had an admixed origin with recent introgressions from other breeds, mainly from Suffolk animals. In conclusion, Brazilian Somali and Brazilian Fat-tail are the most endangered sheep genetic resources in Brazil.

The aim of this study was to evaluate genetic parameters for subjective scores given at the Brazilian Sport Horse (BSH) Stallion Approval by estimating heritability (h

O crescimento alométrico de raças de suínos naturalizados (Nilo, Piau, Moura e Monteiro), machos e fêmeas, foi avaliado em relação à altura do quadril, comprimento do focinho e comprimento da cabeça, usando-se a equação alométrica. As medidas do corpo geralmente apresentam um crescimento mais tardio em relação ao comprimento do focinho e precocemente em relação ao comprimento da cabeça e altura do quadril. Ao comparar as raças de suínos, as fêmeas da raça Moura apresentaram características relacionadas com o crescimento muscular (circunferência torácica, comprimento do corpo e diâmetro longitudinal) mais tardiamente ao comprimento da cabeça. Fêmeas Nilo apresentaram características do comprimento do ombro, comprimento da garupa e a distância interesquiática mais precoce em relação ao comprimento do focinho. Foram encontradas diferenças entre a raça e o sexo no tempo de desenvolvimento para as diferentes partes do corpo, mostrando que os sistemas de criação e utilização desses animais devem ser específicos para cada raça. The allometric growth of male and female naturalized pig breeds (Nilo, Piau, Moura, and Monteiro) was evaluated regarding hip height, snout length, and head length using the allometric equation. Body measurements generally show later growth in relation to the snout length and earlier growth in relation to the head length and hip height. When comparing breeds of pigs, female Moura animals showed later growth of the characteristics linked to muscle (thoracic circumference, body length, and longitudinal diameter) than of head length. Nilo females showed characteristics such as shoulder length, rump length and interschiadic distance earlier than snout length. There were differences between the breed and gender in terms of development times for the different parts of the body, showing that breeding systems and use of these animals have to be specific for each breed.

Made available in DSpace on 2019-10-06T15:48:28Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-08-01 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) In this study we aimed to evaluate the effect of Trichostrongylus colubriformis infection on digesta passage rate, rumen fermentation and lamb performance. Eighteen three-month-old Santa Ines castrated male lambs (16.9 ± 1.43 kg of body weight) were randomly distributed in two experimental treatments: Infected with T. colubriformis (I, n = 9) and Uninfected (U, n = 9). The I lambs received a total of 45,000 L3 larvae of T. colubriformis (5,000 infective larvae, three times per week for three weeks). Daily feed intake was assessed using the I lambs as a reference for their respective pairs on the U group (pair-fed). Weight, body condition score and faeces (stool) samples were obtained every 15 days for 75 days. In both treatments, faecal egg count (FEC), digesta passage rate, rumen fermentation parameters, protozoa count and short chain fatty acids (SCFA) were evaluated. The lambs presented moderate infection (FEC = 620). The retention time of the digesta in the rumen-reticulum segment was lower (P < 0.05) in I lambs. The I lambs presented no inappetence, however, lower concentrations of total SCFA and butyrate, while higher acetate concentration were observed in these lambs (P < 0.05). The present findings highlight that T. colubriformis infection decreased the retention time (solid and liquid content) of the digesta in the rumen-reticulum, as well as negatively affected lamb growth. Laboratório de Nutrição Animal Centro de Energia nuclear na Agricultura (CENA) Universidade de São Paulo Departamento de Cirurgia da Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo Instituto de Biociências Universidade Estadual Paulista (UNESP) Universidade de Brasília/Instituto de Ciências Biológicas, Distrito Federal University of Florida Institute of Food and Agricultural Sciences Department of Animal Sciences, PO Box 110910 Instituto de Biociências Universidade Estadual Paulista (UNESP) FAPESP: 2014/05023-1 FAPESP: 2018/06191-6

Gossypol easily pairs with lysine side chains and enzymes involved in the cellular growth process. The effect of gossypol (a compound present in cotton co-products) in ruminant metabolism and meat quality is not yet clear. This study was undertaken in order to evaluate the effects of cotton co-products in lamb muscle proteome. Twenty-four Santa Inês ram lambs, 5-months old (20.6 ± 1.9 kg), were randomly assigned to four treatments: control (without cottonseed), whole cottonseed, cottonseed meal and high oil cottonseed meal. At 95 days into the experiment, lambs were slaughtered and samples from Longissimus dorsi were collected. Proteins were extracted and analyzed by 2-D electrophoresis. Spots showing a significant effect from the treatment (the “treatment effect”) and present in more than 90 % of the samples were identified using mass spectrometry. Cotton co-products decreased the abundance of aldehyde and malate dehydrogenases, creatine kinase M-type and Adenosine triphosphate (ATP) synthase. They also increased four proteins related to muscle contraction. Thus, feeding cotton co-products to lambs changed the abundance of important muscle proteins. A cotton co-product diet induced a negative impact on the energy supply of muscle cells and, consequently, the abundance of ATP dependent proteins (contractile apparatus) increased, probably in order to offset and maintain muscle function. These proteomic changes can promote our understanding of alterations in the sensorial properties of meat due to cotton co-product diets in further investigations.

The aim of this study was to investigate the effects of castration on carcass characteristics and fatty acid profile of visceral fat and meat from lambs. Eighteen six-month-old Santa Ines male lambs (18.9 ± 2.4 kg of body weight (BW)) were used. Animals were assigned to two treatments according to their sexual condition: uncastrated (U) or castrated (C). During a 98-day experimental period, animals were kept on an Andropogon gayanus grass pasture area of 1 ha and supplemented with 200 g/animal/day of concentrate mixture. Water and mineral salt were available ad libitum. The lambs were weighed fortnightly, and at the last day of the trial, they were slaughtered for evaluation of carcass characteristics and fatty acids profile of perirenal fat and longissimus lumborum muscle samples. Castrated lambs showed higher BW than U during most part of the experiment (p 0.05) (U, 0.74 and C, 0.76%). The cis-9, cis-12 C18:2n-6 (U, 10.6 and C, 6.86%) fatty acid and the PUFA:SFA (saturated fatty acids) ratio (U, 0.36 and C, 0.25) were higher in the muscle of U lambs (p

New breeds can be developed by combining two or more breeds into a composite population, thereby capitalizing on complementarity and retained heterosis. Evaluation of breed formation across generations has not been performed at the molecular level; therefore, we used Brangus cattle (3/8 Brahman x 5/8 Angus) as a model to identify the dynamics of new breed formation across 10 generations. Genotypic data (777,962 SNP) from 68 Brahman, 95 Angus and 59 Brangus prominent AI sires born from 1970 to 2010 were evaluated. Their pedigrees were used to calculate the number of complete Brangus generations using the OptiSel package in R 3.4.2. After standard SNP data filters and linkage disequilibrium pruning, 158,264 SNP genotypes from 220 bulls were chosen. ADMIXTURE analyses were executed using autosomal markers by chromosome, setting K equal to two and three. Brangus animals had 70.4% of Angus composition in all autosomes versus the expected 62.5% (t-test, P < 0.001). When K = 3, the establishment of the Brangus cluster was observed across the genome with a proportional assignment > 0.5. The sex chromosomes deviated from the autosomal pattern. Chromosome X had a high Angus proportion (83%) conserved across generations and Brahman contributed only to 9% of the Y chromosome. Regression analyses of cluster formation among autosomes and across generations showed (P < 0.001) that Angus (β = -0.07) and Brahman (β = -0.03) clusters decreased while Brangus cluster increased (β = 0.10, R(2) = 0.43). Regressions by each autosome followed a similar pattern. Formation of the Brangus cluster became stable after the 5(th) generation. These results provide a view into the evolution of a new breed. [Image: see text]

This study assessed the risks of different management practices to increase pregnancy rate in beef cow-calf systems, aiming at assisting decision-making. The perception of 18 experts on animal sciences regarding the risks of 32 nutritional, breeding, and general management practices applied to increase pregnancy rate were evaluated through questionnaires. The experts were selected by a non-probability sampling of researchers on veterinary and animal sciences. In addition, five farmers and eight technical consultants were also selected. The questionnaire was applied during a face-to-face meeting. The risk of each practice was assessed according to four factors, namely, cost, technical knowledge, operational complexity, and flexibility, and an equation was developed to calculate this risk. The applied method allowed to determine the risk of each practice, obtaining results similar to those previously perceived by the experts. Operational complexity and cost had greater influence on the estimated risks compared with the other factors. Moreover, the increase of one unit in operational complexity and cost increased the perceived risk and the estimated risk scores in 0.43 and 0.28 points, respectively. Overall, the application of general management practices presented lower risk score compared with nutritional and breeding practices, which were not different from which other. Equations to estimate the risks of farm managers should routinely apply management practices to increase the efficiency of cow-calf production systems.

The aim of this study was to explore spatial patterns of genetic structure in sheep breeds sampled in Brazil using Single Nucleotide Polymorphisms (SNPs) in order to evaluate whether or not the genetic and geographic distances are interrelated in this species. Samples genotyped using the 50KSheepSNPChip (Illumina) included 215 animals from eight different sheep breeds. All collection sites were georeferenced and data analyzed to determine geographic patterns from genetic data. To evaluate the correlation between genetic and geographic distances Mantel tests, Allelic Aggregation Index Analyses (AAIA), and spatial autocorrelation were performed. Genetic Landscape Shape procedure and Monmonier’s Algorithm were used to generate graphical visualization of the genetic distances across the landscape. The observed correlation observed between genetic and geographical distances was 0.552 (P

Visual and thermography methods were compared for ectoparasites counting in cattle.Ectoparasites were cooler than the body of the animals in the thermographic images.The correlation between visual and thermographic method was 0.82 for horn flies.Ticks had a lower correlation which depended on hair cover.Thermographic method was more accurate than visual methods for counting external ectoparsites in cattle. The aim of this study was to compare standard visual methods with infrared thermography combined with a program that contrasted points in the image analysis for automatic counting of ectoparasites (ticks and horn flies) in cattle under pasture conditions. Twenty Holstein cows, 10 Devon cows and 10 Braford bulls were used. External parasites were visually counted and thermographic images taken with a FLIRź T300 camera. Images were processed in Quick reportź and parasites also were counted using ImageJź. Ectoparasites were cooler than the body of the animals in the thermographic images and therefore were easily highlighted by Quick reportź program. In the case of ticks, they were especially visible when gravid female ticks were engorged but difficult to observe when hair was long. Horn flies stood out perfectly on thermographic images and were seen down to 3mm in length. The correlation between visual and thermographic combined with a program counts method was 0.82 for horn flies. The correlation between these methods was 0.32 for the ticks on the lateral of the animal and 0.84 for the ticks on the distal of the animal. The regression between visual and thermographic measures for horn fly was highly significant for the three breeds. The use of thermographic images combined with a program for automatic counting was a useful tool and more accurate than the standard visual methods for counting external ectoparsites in cattle.

The Brazilian Sheep Breeders' Association recognizes two varieties of the Morada Nova hair breed, white and red. However, the black variety and/or animals with a pigmented nose has frequently been disqualified from genealogical records. Previous studies suggest that this genetic group might be similar to the red variety. Thus, the aim of this study was to conduct a Genome-wide association study (GWAS) to identify genomic regions related to hair color and confirm the position of black relative to other Morada Nova varieties. After quality controls, 48 animals were genotyped for 45.982 SNPs using the OvineSNP50k BeadChip. Estimated Fst values between white and red animals, white and black, and red and black were 10.78% ( p p p Oar 14 which contains the MC1R gene. Differences between black and red coats are the result of the expression of different alleles of the same gene without directly affecting productive/reproductive characteristics. These two varieties showed low genetic variation, insufficient to define them as different groups, and to increase the breeding herd, the animals with black hair and/or pigmentation of the nose should be used breeding purposes. The results of this study contribute to the discussion of the importance in reconciling conservation, traditional breed standards and breeding of farm animals.

The aim in this study was detect changes in landscape-fragmentation patterns resulting from the expansion of agriculture in the Cerrado region (Brazilian Savanna) during the period 1988–2011. The study area covers 7,559,783.69 ha and is restricted to the limits of the Urucuia Group (Upper Cretaceous), a geological unit formed by sedimentary rocks of continental deposits where there has been intense agricultural expansion due to favorable conditions for the use of mechanized farming. The temporal analysis considered land-use/land-cover data from Landsat TM image classification for the years 1988, 1992, 1996, 2000, 2004, 2008 and 2011. Fragmentation quantification was performed from the morphological spatial pattern analysis (MSPA) and traditional landscape metrics analysis. The calculation of MSPA attributes considered 10 edge widths, between 30 and 300 m. Traditional landscape metrics were obtained from Path Analyst and V-Late software. Change detection in MSPA classes was obtained through cross-tabulation. Cerrado deforestation in the study area increased from 795,502.61 ha in 1988 to 2804,679.75 ha in 2011. The spatial pattern of Cerrado deforestation and fragmentation has a spatial concentration in the western region of the study area that gradually progresses toward the east. Our results emphasize the fragmentation process with a reduction in the following fragmentation indicators over the past two decades: area, number of patches, core, and edge. In addition, the indicators increased in: complexity, size variation, and coreless fragments. Change detection enabled the description of spatial evolution of fragmentation indicators. The results can help define strategies for landscape planning and decision making for conservation priorities.

Objective The aim of this study was to evaluate heat tolerance using heat tolerance indices, physiological, physical, thermographic, and hematological parameters in Santa Ines and Morada Nova sheep breeds in the Federal District, Brazil. Methods Twenty-six adult hair sheep, one and a half years old, from two genetic groups (Santa Ines: 12 males and 4 females; Morada Nova: 7 males and 3 females) were used and data (rectal temperature, respiratory rate, heart rate, skin temperatures; hematological parameters) were collected during three consecutive days, twice a day (morning and afternoon), with a total of six repetitions. Also physical parameters (biometric measurements, skin and hair traits) and heat tolerance indices (temperature-humidity index, Iberia and Benezra) were evaluated. The analyses included analyses of variance, correlation, and principal components with a significance level of 5%. Results The environmental indices, in general, indicate a situation of thermal discomfort for the animals during the afternoon. Breed significantly influenced (p

Morada Nova hair sheep show traits desirable for lamb production especially in extensive production systems in Northeastern Brazil, representing an important genetic resource for producing lamb in semi-arid climates in Brazil and elsewhere. Performance testing has been carried out annually with this breed since 2008. In the present study, Morada Nova sheep from two Brazilian states: Ceara (140 animals) and Sao Paulo (112 animals) were genotyped for a SNP associated with litter size, which is almost only found in Brazilian locally adapted sheep breeds (FecGE). The total observed frequency of FecGE was 0.65, while an increased number of observed heterozygotes was also observed (χ2 = 7.274, p