4,904 results on '"Chuan Liu"'
Search Results
202. High-fat feeding rather than obesity drives taxonomical and functional changes in the gut microbiota in mice
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Liang Xiao, Si Brask Sonne, Qiang Feng, Ning Chen, Zhongkui Xia, Xiaoping Li, Zhiwei Fang, Dongya Zhang, Even Fjære, Lisa Kolden Midtbø, Muriel Derrien, Floor Hugenholtz, Longqing Tang, Junhua Li, Jianfeng Zhang, Chuan Liu, Qin Hao, Ulla Birgitte Vogel, Alicja Mortensen, Michiel Kleerebezem, Tine Rask Licht, Huanming Yang, Jian Wang, Yingrui Li, Manimozhiyan Arumugam, Jun Wang, Lise Madsen, and Karsten Kristiansen
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C57BL/6J mice ,129S6/Sv mice ,Obesity ,High-fat feeding ,Microbiota ,Microbiome ,Microbial ecology ,QR100-130 - Abstract
Abstract Background It is well known that the microbiota of high-fat (HF) diet-induced obese mice differs from that of lean mice, but to what extent, this difference reflects the obese state or the diet is unclear. To dissociate changes in the gut microbiota associated with high HF feeding from those associated with obesity, we took advantage of the different susceptibility of C57BL/6JBomTac (BL6) and 129S6/SvEvTac (Sv129) mice to diet-induced obesity and of their different responses to inhibition of cyclooxygenase (COX) activity, where inhibition of COX activity in BL6 mice prevents HF diet-induced obesity, but in Sv129 mice accentuates obesity. Results Using HiSeq-based whole genome sequencing, we identified taxonomic and functional differences in the gut microbiota of the two mouse strains fed regular low-fat or HF diets with or without supplementation with the COX-inhibitor, indomethacin. HF feeding rather than obesity development led to distinct changes in the gut microbiota. We observed a robust increase in alpha diversity, gene count, abundance of genera known to be butyrate producers, and abundance of genes involved in butyrate production in Sv129 mice compared to BL6 mice fed either a LF or a HF diet. Conversely, the abundance of genes involved in propionate metabolism, associated with increased energy harvest, was higher in BL6 mice than Sv129 mice. Conclusions The changes in the composition of the gut microbiota were predominantly driven by high-fat feeding rather than reflecting the obese state of the mice. Differences in the abundance of butyrate and propionate producing bacteria in the gut may at least in part contribute to the observed differences in obesity propensity in Sv129 and BL6 mice.
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- 2017
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203. Visual time-sensitive SAR target detection technology based on human brain mapping
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Kuiying Yin, Qixue Li, Jimin Liang, Chuan Liu, Chang Niu, and Zhongbao Wang
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man-machine systems ,radar imaging ,medical signal processing ,object detection ,feature extraction ,brain ,synthetic aperture radar ,human brain mapping ,expert brain central responding messages ,sar images ,fusing detection model ,sar targets ,expert on-line brain–machine ,higher detection efficiency ,visual time-sensitive sar target detection technology ,synthetic aperture radar images ,Engineering (General). Civil engineering (General) ,TA1-2040 - Abstract
To detect targets from synthetic aperture radar (SAR) images, this study describes a visual time-sensitive SAR target detection technology based on human brain mapping. Expert brain central responding messages are introduced into the automatic analysis method of SAR images. A fusing detection model between brain central responding messages and features of SAR targets is built by expert on-line brain–machine combined modelling. The experimental results show that the technology paper proposed succeeds in automatically detecting and recognising targets of SAR images in actual combat environment, and owns better detection results and higher detection efficiency than DARPA's work. In conclusion, this study proposes an ideal man–machine fusion technology which is worthy of high research value and broad application prospect.
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- 2019
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204. Radiomics Analysis of Dynamic Contrast-Enhanced Magnetic Resonance Imaging for the Prediction of Sentinel Lymph Node Metastasis in Breast Cancer
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Jia Liu, Dong Sun, Linli Chen, Zheng Fang, Weixiang Song, Dajing Guo, Tiangen Ni, Chuan Liu, Lin Feng, Yuwei Xia, Xiong Zhang, and Chuanming Li
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breast cancer ,DCE-MRI ,radiomics ,sentinel lymph node metastasis ,automatic machine learning ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Purpose: To investigate whether a combination of radiomics and automatic machine learning applied to dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) of primary breast cancer can non-invasively predict axillary sentinel lymph node (SLN) metastasis.Methods: 62 patients who received a DCE-MRI breast scan were enrolled. Tumor resection and sentinel lymph node (SLN) biopsy were performed within 1 week after the DCE-MRI examination. According to the time signal intensity curve, the volumes of interest (VOIs) were delineated on the whole tumor in the images with the strongest enhanced phase. Datasets were randomly divided into two sets including a training set (~80%) and a validation set (~20%). A total of 1,409 quantitative imaging features were extracted from each VOI. The select K best and least absolute shrinkage and selection operator (Lasso) were used to obtain the optimal features. Three classification models based on the logistic regression (LR), XGboost, and support vector machine (SVM) classifiers were constructed. Receiver Operating Curve (ROC) analysis was used to analyze the prediction performance of the models. Both feature selection and models construction were firstly performed in the training set, then were further tested in the validation set by the same thresholds.Results: There is no significant difference between all clinical and pathological variables in breast cancer patients with and without SLN metastasis (P > 0.05), except histological grade (P = 0.03). Six features were obtained as optimal features for models construction. In the validation set, with respect to the accuracy and MSE, the SVM demonstrated the highest performance, with an accuracy, AUC, sensitivity (for positive SLN), specificity (for positive SLN) and Mean Squared Error (MSE) of 0.85, 0.83, 0.71, 1, 0.26, respectively.Conclusions: We demonstrated the feasibility of combining artificial intelligence and radiomics from DCE-MRI of primary tumors to predict axillary SLN metastasis in breast cancer. This non-invasive approach could be very promising in application.
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- 2019
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205. Trimetazidine attenuates high-altitude fatigue and cardiorespiratory fitness impairment: A randomized double-blinded placebo-controlled clinical trial
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Jie Yang, Laiping Zhang, Chuan Liu, Jihang Zhang, Shiyong Yu, Jie Yu, Shizhu Bian, Sanjiu Yu, Chen Zhang, and Lan Huang
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Trimetazidine ,Cardiorespiratory fitness ,High altitude ,Fatigue ,Cardiac function ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Trimetazidine (TMZ) has been shown to optimize myocardial energy metabolism and is a common anti-ischemic agent. Our trial (ChiCTR-TRC-13003298) aimed to explore whether TMZ has any preventive effect on high-altitude fatigue (HAF), cardiac function and cardiorespiratory fitness upon acute high-altitude exposure and how it works on HAF. Thirty-nine healthy young subjects were enrolled in a randomized double-blinded placebo-controlled trial and were randomized to take oral TMZ (n = 20) or placebo (n = 19), 20 mg tid, 14 days prior to departure until the end of study. The 2018 Lake Louise Score questionnaire, echocardiography, assessments of physical working capacity, circulating markers of myocardial energy metabolism and fatigue were performed both before departure and arrival at highland. At follow-up, TMZ significantly reduced the incidence of HAF (p = 0.038), reversed cardiorespiratory fitness impairment, decreased left ventricular end-systolic volume (LVESV, p = 0.032) and enhanced left ventricular ejection fraction (LVEF, p = 0.015) at highland. Relative to the placebo group, the TMZ group had significantly lower LDH (p = 0.025) and lactate levels before (p
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- 2019
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206. Systematic Analysis of Drug Vulnerabilities Conferred by Tumor Suppressor Loss
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Hongyu Ding, Jie Zhao, Yanli Zhang, Jiao Yu, Mingxian Liu, Xiaoxi Li, Liang Xu, Minghui Lin, Chuan Liu, Zhengjin He, Shishuang Chen, and Hai Jiang
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Biology (General) ,QH301-705.5 - Abstract
Summary: In addition to oncogene inhibition, targeting tumor suppressor deficiency could provide potential venues for precision cancer medicine. However, the full spectrum of drug vulnerability conferred by tumor suppressor loss remains unclear. We systematically analyzed how loss of 59 common tumor suppressors each affected cellular sensitivity to 26 different types of anticancer therapeutics. The experiments were performed in a one-gene, one-drug manner, and through such a large gene-drug iteration study, we were able to generate a drug sensitivity map that describes numerous examples of drug resistance or hypersensitivity conferred by tumor suppressor loss. We further delineated the mechanisms of several gene-drug interactions, showing that loss of tumor suppressors could modify drug sensitivity at various steps of drug action. This systematic drug sensitivity map highlights potential drug vulnerabilities associated with tumor suppressor loss, which may help expand precision cancer medicine on the basis of tumor suppressor status. : Ding et al. describe how deficiencies of tumor suppressors affect cellular sensitivities to 26 types of anticancer drugs. The study reveals tumor suppressor-drug interactions potentially useful for cancer therapy. Keywords: tumor suppressor, drug sensitivity, SETD2, CREBBP, BAP1
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- 2019
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207. Analysis of Ultrahigh Apparent Mobility in Oxide Field‐Effect Transistors
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Changdong Chen, Bo‐Ru Yang, Gongtan Li, Hang Zhou, Bolong Huang, Qian Wu, Runze Zhan, Yong‐Young Noh, Takeo Minari, Shengdong Zhang, Shaozhi Deng, Henning Sirringhaus, and Chuan Liu
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carrier mobility ,doping ,four‐probe measurement ,surface potential scanning ,thin‐film transistors ,Science - Abstract
Abstract For newly developed semiconductors, obtaining high‐performance transistors and identifying carrier mobility have been hot and important issues. Here, large‐area fabrications and thorough analysis of InGaZnO transistors with enhanced current by simple encapsulations are reported. The enhancement in the drain current and on–off ratio is remarkable in the long‐channel devices (e.g., 40 times in 200 µm long transistors) but becomes much less pronounced in short‐channel devices (e.g., 2 times in 5 µm long transistors), which limits its application to the display industry. Combining gated four‐probe measurements, scanning Kelvin‐probe microscopy, secondary ion mass spectrometry, X‐ray photoelectron spectroscopy, and device simulations, it is revealed that the enhanced apparent mobility up to several tens of times is attributed to the stabilized hydrogens in the middle area forming a degenerated channel area while that near the source‐drain contacts are merely doped, which causes artifact in mobility extraction. The studies demonstrate the use of hydrogens to remarkably enhance performance of oxide transistors by inducing a new mode of device operation. Also, this study shows clearly that a thorough analysis is necessary to understand the origin of very high apparent mobilities in thin‐film transistors or field‐effect transistors with advanced semiconductors.
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- 2019
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208. Association Between Single Nucleotide Polymorphisms in PPARA and EPAS1 Genes and High-Altitude Appetite Loss in Chinese Young Men
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Wenxu Pan, Chuan Liu, Jihang Zhang, Xubin Gao, Shiyong Yu, Hu Tan, Jie Yu, Dehui Qian, Jiabei Li, Shizhu Bian, Jie Yang, Chen Zhang, Lan Huang, and Jun Jin
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high altitude ,appetite loss ,hypoxia ,PPARA ,EPAS1 ,single nucleotide polymorphism ,Physiology ,QP1-981 - Abstract
Appetite loss is a common symptom that occurs in high altitude (HA) for lowlanders. Previous studies indicated that hypoxia is the initiating vital factor of HA appetite loss. PPARA, EPAS1, EGLN1, HIF1A, HIF1AN, and NFE2L2 play important roles in hypoxic responses. We aimed to explore the association of these hypoxia-related gene polymorphisms with HA appetite loss. In this study, we enrolled 416 young men who rapidly ascended to Lhasa (3700 m) from Chengdu (
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- 2019
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209. Design of Sewage Treatment Plants for High-Density Urban Reclamation Land
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Chuan Liu, Chew Tin Lee, and Hao Zhang
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Chemical engineering ,TP155-156 ,Computer engineering. Computer hardware ,TK7885-7895 - Abstract
The success of any coastal reclamation project depends strongly on a sound planning and design practices. Due to the land use limitations, sewage treatment plant (STP) of the reclamation land should be fully studied to save land and reduce its adverse effects on the environment. This research focuses on the selection of biological treatment process for a novel STP, as various pollutants of the sewage can be effectively removed via the biological process. The paper presents a design of the STP implemented in Johor as an example, to achieve the goal of ‘zero discharge’ in the reclaimed wetland. The domestic sewage is treated, recycled and reused in the artificial wetland as an ecological green water to achieve Class IIB standard (effluent quality). The novel STP design comprised of the Immobilized Aerobic Biofilm (BioAX) and Mass Bio System (MBS) was compared against the conventional Sequencing Batch Reactor (SBR) process. Combination BioAX + MBS process is advantageous due to significantly less land needed, lower power consumption and lower sludge generation. The system was demonstrated as a viable process to treat the sewage wastewater in the reclamation land to meet Class IIB discharge at a lower environmental footprint, saving up to 50 % of the area.
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- 2019
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210. A Novel Bio-film Wastewater Treatment System using Encapsulated Microbes
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Hao Zhang, Chuan Liu, Chew Tin Lee, and Zhenjia Zhang
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Chemical engineering ,TP155-156 ,Computer engineering. Computer hardware ,TK7885-7895 - Abstract
The untreated or incomplete disposal of nitrogen pollutants in the wastewater can cause great harm to the environment. A large amount of ammonia-nitrogen (NH3-N) will consume dissolved oxygen and cause eutrophication. The conventional wastewater treatment process in Malaysia is not efficient enough to remove the NH3-N. Most of the sewage treatment plants (STPs) in Malaysia are open structures which occupy a large area and emit stench. The existing STPs are not suitable for construction in the compact urban areas and some of these STPs operation are costly. This study designs a novel biological water purification system using a bio-film reactor with encapsulated microbes that is known as Mass Bio System (MBS). The MBS had been used in the east of Asia to treat high concentration of NH3-N in the industrial wastewater with high efficiency. MBS has not been reported to treat domestic wastewater. This paper reports the adaptability and verification of the new sewage treatment system based on MBS to treat domestic wastewater in Malaysia. The study will dynamically monitor the key parameters and compared with the conventional extended aeration (EA) process that is the most common STP applied in Malaysia using the same influent conditions. The aim is to verify that the MBS can treat the wastewater to meet the local emission standard where the water discharge shows the characteristics well below the discharge limits. The study showed that the average effluent and removal efficiency of NH3-N was approximately 2 mg/L and 88.6 % (with maximum 99.7 % removal). All evaluated parameters in the MBS showed stability of effluent characteristics and met the requirements of Malaysian sewerage industry guidelines. MBS also offers the co-benefits of energy, and space saving than the conventional process. The process is recommended as an efficient sewage treatment system in Malaysia and other countries with similar climatic conditions.
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- 2019
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211. Genome-Wide Identification and Characterization of the ALOG Domain Genes in Rice
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Na Li, Yang Wang, Jing Lu, and Chuan Liu
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Genetics ,QH426-470 - Abstract
The ALOG domain genes, named after the Arabidopsis LSH1 and Oryza G1 (ALOG) proteins, have frequently been reported as key developmental regulators in rice and Arabidopsis. However, the investigation of the ALOG gene family is limited. Here, we conducted a genome-wide investigation of the ALOG gene family in rice and six other species. In total, eighty-four ALOG domain genes were identified from the seven species, of which fourteen ALOG domain genes (OsG1/G1Ls) were identified in the rice genome. The fourteen OsG1/G1Ls were unevenly distributed on eight chromosomes, and we found that eight segmental duplications contributed to the expansion of OsG1/G1Ls in the rice genome. The eighty-four ALOG family genes from seven species were classified into six clusters, and the ALOG domain-defined motifs 1, 2, and 3 were highly conserved across species according to the phylogenetic and structural analysis. However, the newly identified motifs 4 and 5 were only present in monocots, indicating a specified function in monocots. Moreover, OsG1/G1Ls exhibited tissue-specific expression patterns. Coexpression analysis suggested that OsG1 integrates OsMADS50 and the downstream MADS-box genes, such as OsMADS1, to regulate the development of rice inflorescence; OsG1L7 potentially associates with OsMADS22 and OsMADS55 to regulate stem elongation. In addition, comparative expression analysis revealed the conserved biological functions of ALOG family genes among rice, maize, and Arabidopsis. These results have shed light on the functional study of ALOG family genes in rice and other plants.
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- 2019
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212. Enhanced Fire Safety of Rigid Polyurethane Foam via Synergistic Effect of Phosphorus/Nitrogen Compounds and Expandable Graphite
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Chuan Liu, Ping Zhang, Yongqian Shi, Xiaohui Rao, Suncheng Cai, Libi Fu, Yuezhan Feng, Liancong Wang, Xueqin Zheng, and Wei Yang
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flame retardancy ,rigid polyurethane foam ,phosphorus-nitrogen compounds ,heat release ,synergistic effect ,Organic chemistry ,QD241-441 - Abstract
In order to explore highly efficient flame-retardant rigid polyurethane foam (RPUF), phosphorus/nitrogen compounds and expandable graphite (EG) were successfully incorporated into RPUF by a free one-spot method. The combustion results showed that the fire safety of the RPUF samples was remarkably improved by the addition of phosphoric/nitrogen compounds and EG. With the incorporation of 22.4 wt.% phosphorus/nitrogen compounds and 3.2 wt.% EG, the RPUF composites achieved UL-94 V-0 rating. Besides, the total heat release and total smoke release of RPUF composites were reduced by 29.6% and 32.4% respectively, compared to those of the pure RPUF sample. PO• and PO2• together with nonflammable gaseous products were evolved from phosphoric/nitrogen compounds in the gas phase, which quenched the flammable free radicals in the matrix and diluted the concentration of combustible gaseous products generated from PRUF during combustion. The compact char residues which acted as excellent physical barriers were formed by catalysis of EG and phosphoric/nitrogen compounds in the condense phase. The fire hazard of RPUF was significantly reduced by the synergistic effect of phosphorus-nitrogen compounds and EG. This work provides a promising strategy to enhance the fire safety of RPUF.
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- 2020
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213. Cadmium Exposure Enhances Bisphenol A-Induced Genotoxicity through 8-Oxoguanine-DNA Glycosylase-1 OGG1 Inhibition in NIH3T3 Fibroblast Cells
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Zhi-Yu Chen, Chuan Liu, Yong-hui Lu, Ling-Ling Yang, Min Li, Min-Di He, Chun-Hai Chen, Lei Zhang, Zheng-Ping Yu, and Zhou Zhou
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Cadmium ,Bisphenol A ,Genotoxicity ,Cytotoxicity ,OGG1 ,Physiology ,QP1-981 ,Biochemistry ,QD415-436 - Abstract
Background: Both cadmium (Cd) and bisphenol A (BPA) are commonly encountered in humans' daily activities, but their combined genotoxic effects remain unclear. Methods: In the present study, we exposed a mouse embryonic fibroblast cell line (NIH3T3) to Cd for 24 h, followed by a 24 h BPA exposure to evaluate toxicity. The cytotoxicity was evaluated by viability with CCK-8 assay and lactate dehydrogenase (LDH) release. Reactive oxygen species (ROS) production was measured by 2′,7′-dichlorofluorescein diacetate (DCFH-DA). And DNA damage was measured by 8-hydroxydeoxyguanosine (8-OHdG), phosphorylated H2AX (γH2AX) and the comet assay. The flow cytometry was used to detect cell cycle distribution, and apoptosis was determined by TUNEL assay and western blot against poly-ADP-ribose polymerase (PARP). Results: The results showed that Cd or BPA treatments alone (with the exception of BPA exposure at 50 μM) did not alter cell viability. However, pre-treatment with Cd aggravated the BPA-induced reduction in cell viability; increased BPA-induced LDH release, ROS production, DNA damage and G2 phase arrest; and elevated BPA-induced TUNEL-positive cells and the expression levels of cleaved PARP. Cd exposure concurrently decreased the expression of 8-oxoguanine-DNA glycosylase-1 (OGG1), whereas OGG1 over-expression abolished the enhancement of Cd on BPA-induced genotoxicity and cytotoxicity. Conclusion: These findings indicate that Cd exposure aggravates BPA-induced genotoxicity and cytotoxicity through OGG1 inhibition.
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- 2016
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214. Crystal Structure and Substrate Specificity of PTPN12
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Hui Li, Fan Yang, Chunhua Liu, Peng Xiao, Yunfei Xu, Zonglai Liang, Chuan Liu, Hongmei Wang, Wenjun Wang, Wenshuai Zheng, Wei Zhang, Xiaoyun Ma, Dongfang He, Xiaoyuan Song, Fuai Cui, Zhigang Xu, Fan Yi, Jin-Peng Sun, and Xiao Yu
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Biology (General) ,QH301-705.5 - Abstract
PTPN12 is an important tumor suppressor that plays critical roles in various physiological processes. However, the molecular basis underlying the substrate specificity of PTPN12 remains uncertain. Here, enzymological and crystallographic studies have enabled us to identify two distinct structural features that are crucial determinants of PTPN12 substrate specificity: the pY+1 site binding pocket and specific basic charged residues along its surface loops. Key structurally plastic regions and specific residues in PTPN12 enabled recognition of different HER2 phosphorylation sites and regulated specific PTPN12 functions. In addition, the structure of PTPN12 revealed a CDK2 phosphorylation site in a specific PTPN12 loop. Taken together, our results not only provide the working mechanisms of PTPN12 for desphosphorylation of its substrates but will also help in designing specific inhibitors of PTPN12.
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- 2016
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215. Topological susceptibility near Tc in SU(3) gauge theory
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Guang-Yi Xiong, Jian-Bo Zhang, Ying Chen, Chuan Liu, Yu-Bin Liu, and Jian-Ping Ma
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Topological charge ,Finite temperature ,Topological susceptibility ,Topological cumulant ,Physics ,QC1-999 - Abstract
Topological charge susceptibility χt for pure gauge SU(3) theory at finite temperature is studied using anisotropic lattices. The over-improved stout-link smoothing method is utilized to calculate the topological charge. Near the phase transition point we find a rapid declining behavior for χt with values decreasing from (188(1) MeV)4 to (67(3) MeV)4 as the temperature increased from zero temperature to 1.9Tc which demonstrates the existence of topological excitations far above Tc. The 4th order cumulant c4 of topological charge, as well as the ratio c4/χt is also investigated. Results of c4 show step-like behavior near Tc while the ratio at high temperature agrees with the value as predicted by the diluted instanton gas model.
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- 2016
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216. Industry regulation, competition, and the dynamics of productivity growth: evidence from China’s iron and steel industry
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Jianbai Huang, Chuan Liu, Daguo Lü, and Xiaoping Li
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productivity dynamics ,industry regulation ,market competition ,China ,Economic theory. Demography ,HB1-3840 - Abstract
This study aims to investigate the characteristics of the efficient evolution of China’s iron and steel industry against the backdrop of limited openness and regulation and to discuss the important effects of regulatory policy and total factor productivity (TFP) of China’s iron and steel industry. The method adopted the Cobb–Douglas production function combined with a semi-parametric method to decompose productivity. This study based on the micro-level dataset of iron and steel enterprises in the database of China’s industrial enterprises between 1998 and 2007 that investigated the intrinsic correlation among corporate entry and exit, market competition of existing enterprises, and TFP growth in the iron and steel industry. The results of the research show that the entry-exit would promote the growth of the aggregate productivity, while the low efficiency of resource allocation would significantly inhibit the TFP growth. The basic conclusion is that, with substantial government intervention in enterprise investments, market competition may not promote optimal resource allocation efficiency in China’s iron and steel industry, but make the allocation less efficient.
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- 2015
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217. Change of intraocular pressure and intracranial pressure during hemodialysis
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Chuan Liu and He-Zheng Zhou
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hemodialysis ,intraocular pressure ,intracranial pressure ,trans-lamina cribrosa pressure difference ,Ophthalmology ,RE1-994 - Abstract
Hemodialysis is an important therapeutic method of acute and chronic renal failure and other serious diseases.Many studies have shown that hemodialysis may cause changes of intraocular pressure and intracranial pressure. It may provide new approach for the establishment of glaucoma model by studying intraocular pressure and intracranial pressure changes during hemodialysis, which may contribute to the research on glaucomatous optic nerve damage. In this article, we reviewed the change of intraocular pressure and intracranial pressure during hemodialysis.
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- 2015
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218. Highly Effective Flame-Retardant Rigid Polyurethane Foams: Fabrication and Applications in Inhibition of Coal Combustion
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Liancong Wang, Benjamin Tawiah, Yongqian Shi, Suncheng Cai, Xiaohui Rao, Chuan Liu, Ye Yang, Fuqiang Yang, Bin Yu, Yuntao Liang, and Libi Fu
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polyurethane foam ,intumescent flame-retardant ,coal combustion ,expandable graphite ,phosphorus ,Organic chemistry ,QD241-441 - Abstract
The extemporaneous combustion of coal remains a major threat to safety in coal mines because such fire accidents result in casualties and significant property loss, as well as serious environmental pollution. This work proposed the fabrication of flame-retardant rigid polyurethane foam (RPUF) containing expandable graphite as char expander/sealant with melamine phosphosphate and 2-carboxyethyl (phenyl)phosphinic acid as char inducer and radical trapping agents. The as-prepared RPUF successfully inhibited coal combustion by forming thermally stable high graphitic content expandable intumescent char sealing over the coal. The RPUF achieved UL-94 V-0 rating in addition to significant reductions in peak heat release, total heat release, and CO and CO2 yields. The external and the internal residual char structure was studied by X-ray photoelectron spectra, Raman spectroscopy, and real-time Fourier transform infrared spectra techniques, and a flame-retardant mode of action has been proposed. This work provides important insight into a facile fabrication of highly efficient and economical flame-retardant RPUF to inhibit the spontaneous combustion of coal.
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- 2019
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219. The Value of Ozone in CT-Guided Drainage of Multiloculated Pyogenic Liver Abscesses: A Randomized Controlled Study
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Bing Li, Chuan Liu, Lang Wang, Yang Li, Yong Du, Chuan Zhang, Xiao-xue Xu, and Han Feng Yang
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Diseases of the digestive system. Gastroenterology ,RC799-869 - Abstract
Objective. This study was designed to compare the effects of catheter drainage alone and combined with ozone in the management of multiloculated pyogenic liver abscess (PLA). Methods. The prospective study included 60 patients diagnosed with multiloculated PLA. All patients were randomly divided into two groups: catheter drainage alone (group I) and catheter drainage combined with ozone (group II). Drainage was considered successful when (1) the abscess cavity was drained and (2) clinical symptoms were resolved. Kruskal-Wallis nonparametric test was used to compare the success rates, length of stay (LOS), and need for further surgery of the two groups. P
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- 2018
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220. The expression of APE1 in triple-negative breast cancer and its effect on drug sensitivity of olaparib
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Tianran Chen, Chuan Liu, Heng Lu, Mingzhen Yin, Changjuan Shao, Xiaoding Hu, Jiaxue Wu, and Yajie Wang
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Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Triple-negative breast cancer is a kind of breast cancer with poor prognosis and special biological behavior, which lacked endocrine therapy and targeted therapy. We investigate the effect of human APE1 (apurinic/apyrimidyl endonuclease 1), a rate-limiting enzyme of base excision repair, on the prognosis in triple-negative breast cancer and drug sensitivity of olaparib. The expression of APE1 was detected by immunohistochemistry in the triple-negative breast cancer tissues and its effect on survival of triple-negative breast cancer patients was followed. To find whether APE1 effect the drug sensitivity in triple-negative breast cancer cells, the APE1-knockout HCC1937 cell line (triple-negative breast cancer cell line) was established by CRISPR/Cas9 system. Then, we use the wild-type and knockout one to test the drug sensitivity of olaparib. The expression of APE1 in triple-negative breast cancer tissues was significantly higher than that in the adjacent tissues (85.6% vs 14.4%) and its expression was related to tumor size (p < 0.05). We also found that it is an independent prognostic factor in patients with triple-negative breast cancer (overall survival, p = 0.01). In vitro assay, the half maximal inhibitory concentration of olaparib in HCC1937-APE1-KO was significantly increased (17.22 vs 91.85 μM) compared to the wild type. The growth curve showed that olaparib had a stronger lethality on HCC1937 compared to HCC1937- APE1-KO (p < 0.05 on day 3). HCC1937 resulted in more mitotic G2/M arrest and increased apoptosis rate after treatment with 40 μM of olaparib, while HCC1937-APE1-KO did not change significantly. When HCC1937 was treated with different concentrations of olaparib, it was found that APE1 expression decreased more significantly at 15 μM of olaparib was. In HCC1937-APE1-KO, the expression of endogenous poly (ADP-ribose) polymerase 1 was also less than that of HCC1937. These results suggested that the expression of APE1 was an important basis for the maintenance of poly (ADP-ribose) polymerase 1, and the deletion of APE1 may be related to the resistance of olaparib.
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- 2017
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221. 8-oxoG DNA Glycosylase-1 Inhibition Sensitizes Neuro-2a Cells to Oxidative DNA Base Damage Induced by 900 MHz Radiofrequency Electromagnetic Radiation
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Xiaoya Wang, Chuan Liu, Qinglong Ma, Wei Feng, Lingling Yang, Yonghui Lu, Zhou Zhou, Zhengping Yu, Wei Li, and Lei Zhang
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DNA base damage ,OGG1 ,Neuro-2a cells ,Radiofrequency electromagnetic fields ,Reactive oxygen species ,Physiology ,QP1-981 ,Biochemistry ,QD415-436 - Abstract
Background/Aims: The purpose of this study was to explore the in vitro putative genotoxicity during exposure of Neuro-2a cells to radiofrequency electromagnetic fields (RF-EMFs) with or without silencing of 8-oxoG DNA glycosylase-1 (OGG1). Methods: Neuro-2a cells treated with or without OGG1 siRNA were exposed to 900 MHz Global System for Mobile Communication (GSM) Talk signals continuously at a specific absorption rate (SAR) of 0, 0.5, 1 or 2 W/kg for 24 h. DNA strand breakage and DNA base damage were measured by the alkaline comet assay and a modified comet assay using formamidopyrimidine DNA glycosylase (FPG), respectively. Reactive oxygen species (ROS) levels and cell viability were monitored using the non-fluorescent probe 2, 7-dichlorofluorescein diacetate (DCFH-DA) and CCK-8 assay. Results: Exposure to 900 MHz RF-EMFs with insufficient energy could induce oxidative DNA base damage in Neuro-2a cells. These increases were concomitant with similar increases in the generation of reactive oxygen species (ROS). Without OGG1 siRNA, 2 W/kg RF-EMFs induced oxidative DNA base damage in Neuro-2a cells. Interestingly, with OGG1 siRNA, RF-EMFs could cause DNA base damage in Neuro-2a cells as low as 1 W/kg. However, neither DNA strand breakage nor altered cell viability was observed. Conclusion: Even if further studies remain conducted we support the hypothesis that OGG1 is involved in the process of DNA base repair and may play a pivotal role in protecting DNA bases from RF-EMF induced oxidative damage.
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- 2015
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222. DS-GL: Advancing Graph Learning via Harnessing Nature's Power within Scalable Dynamical Systems.
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Ruibing Song, Chunshu Wu, Chuan Liu 0001, Ang Li 0006, Michael C. Huang 0001, and Tong Geng
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- 2024
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223. Sociotechnical Transformation in the Decade of Healthy Ageing to empower the Silver Economy: Bridging the Silver Divide through Social and Digital Inclusion.
- Author
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Anastasija Nikiforova, Hsien-Lee Tseng, Sidra Azmat Butt, Dirk Draheim, and Li-Chuan Liu
- Published
- 2024
- Full Text
- View/download PDF
224. An Electricity Theft Identification Method by Fusing Clustering and Improved Sparrow Search Algorithm
- Author
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Jian, Lai, Zongyao, Wang, Bing, Kang, Zhihao, Xu, Guili, Ding, Chuan, Liu, Angrisani, Leopoldo, Series Editor, Arteaga, Marco, Series Editor, Chakraborty, Samarjit, Series Editor, Chen, Jiming, Series Editor, Chen, Shanben, Series Editor, Chen, Tan Kay, Series Editor, Dillmann, Rüdiger, Series Editor, Duan, Haibin, Series Editor, Ferrari, Gianluigi, Series Editor, Ferre, Manuel, Series Editor, Jabbari, Faryar, Series Editor, Jia, Limin, Series Editor, Kacprzyk, Janusz, Series Editor, Khamis, Alaa, Series Editor, Kroeger, Torsten, Series Editor, Li, Yong, Series Editor, Liang, Qilian, Series Editor, Martín, Ferran, Series Editor, Ming, Tan Cher, Series Editor, Minker, Wolfgang, Series Editor, Misra, Pradeep, Series Editor, Mukhopadhyay, Subhas, Series Editor, Ning, Cun-Zheng, Series Editor, Nishida, Toyoaki, Series Editor, Oneto, Luca, Series Editor, Panigrahi, Bijaya Ketan, Series Editor, Pascucci, Federica, Series Editor, Qin, Yong, Series Editor, Seng, Gan Woon, Series Editor, Speidel, Joachim, Series Editor, Veiga, Germano, Series Editor, Wu, Haitao, Series Editor, Zamboni, Walter, Series Editor, Tan, Kay Chen, Series Editor, Yang, Qingxin, editor, Li, Zewen, editor, and Luo, An, editor
- Published
- 2024
- Full Text
- View/download PDF
225. Meta-analysis of laparoscopic vs. open resection of gastric gastrointestinal stromal tumors.
- Author
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Liangying Ye, Xiaojing Wu, Tongwei Wu, Qijing Wu, Zhao Liu, Chuan Liu, Sen Li, and Tao Chen
- Subjects
Medicine ,Science - Abstract
This meta-analysis compared laparoscopic surgery (LAP) and open resection (OPEN) for the treatment of gastric gastrointestinal stromal tumors (GISTs) with regard to feasibility and safety.We searched PubMed, Embase, and Web of Science for studies published before March 2016 comparing the LAP and OPEN procedures for GISTs. RevMan 5.1 software was used for the meta-analysis.In total, 28 studies met the inclusion criteria for the meta-analysis. The mean tumor sizes in the OPEN and LAP groups were 4.54 and 5.67 cm. Compared with the OPEN patients, the LAP patients experienced shorter surgical times (P = 0.05), less blood loss (P5 cm), the present study did not report significant differences in operation time (P = 0.93), postoperative complications (P = 0.30), or recurrence rate (P = 0.61) between the two groups, though LAP was associated with favorable results regarding blood loss (P = 0.03) and hospital stay (P5 cm, no significant difference was detected between LAP and OPEN if patient selection and intraoperative decisions were carefully considered.
- Published
- 2017
- Full Text
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226. TiO2 nanowire-templated hierarchical nanowire network as water-repelling coating
- Author
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Tian Hang, Hui-Jiuan Chen, Shuai Xiao, Chengduan Yang, Meiwan Chen, Jun Tao, Han-ping Shieh, Bo-ru Yang, Chuan Liu, and Xi Xie
- Subjects
micro-nanoscale structure ,hierarchical nanowires ,self-cleaning surfaces ,superhydrophobic coatings ,nanowire thin film ,Science - Abstract
Extraordinary water-repelling properties of superhydrophobic surfaces make them novel candidates for a great variety of potential applications. A general approach to achieve superhydrophobicity requires low-energy coating on the surface and roughness on nano- and micrometre scale. However, typical construction of superhydrophobic surfaces with micro-nano structure through top-down fabrication is restricted by sophisticated fabrication techniques and limited choices of substrate materials. Micro-nanoscale topographies templated by conventional microparticles through surface coating may produce large variations in roughness and uncontrollable defects, resulting in poorly controlled surface morphology and wettability. In this work, micro-nanoscale hierarchical nanowire network was fabricated to construct self-cleaning coating using one-dimensional TiO2 nanowires as microscale templates. Hierarchical structure with homogeneous morphology was achieved by branching ZnO nanowires on the TiO2 nanowire backbones through hydrothermal reaction. The hierarchical nanowire network displayed homogeneous micro/nano-topography, in contrast to hierarchical structure templated by traditional microparticles. This hierarchical nanowire network film exhibited high repellency to both water and cell culture medium after functionalization with fluorinated organic molecules. The hierarchical structure templated by TiO2 nanowire coating significantly increased the surface superhydrophobicity compared to vertical ZnO nanowires with nanotopography alone. Our results demonstrated a promising strategy of using nanowires as microscale templates for the rational design of hierarchical coatings with desired superhydrophobicity that can also be applied to various substrate materials.
- Published
- 2017
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227. Beclin 1 Expression in Ovarian Tissues and Its Effects on Ovarian Cancer Prognosis
- Author
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Mingbo Cai, Zhenhua Hu, Juanjuan Liu, Jian Gao, Chuan Liu, Dawo Liu, Mingzi Tan, Danye Zhang, and Bei Lin
- Subjects
autophagy ,Beclin 1 ,ovarian cancer ,prognosis ,overall survival ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Beclin 1 is an autophagy-associated protein involved in apoptosis and drug resistance, as well as various malignancies. We investigated the expression of Beclin 1 protein in ovarian epithelial tissues and correlated it with the prognosis of ovarian cancer. Beclin 1 protein expression was determined using immunohistochemistry in 148 patients with ovarian epithelial cancer, 26 with ovarian borderline tumor, 25 with benign ovarian tumor, and 30 with normal ovarian tissue. The relationships between Beclin 1 protein expression and ovarian cancer pathological characteristics were analyzed. The risk factors for ovarian cancer prognosis were analyzed using Cox’s regression model. A survival curve was plotted from the follow-up data of 93 patients with ovarian cancer to analyze the effects of Beclin 1 expression on the prognosis of ovarian cancer. The positive rates of Beclin 1 were significantly higher in ovarian epithelial cancer (148) and borderline tumor (26) than in benign ovarian tumor (25) or normal ovarian tissue (30) (all p < 0.001). The surgical stage and Beclin 1 expression were both independent risk factors for ovarian cancer prognosis (both p < 0.05). Patients with high Beclin 1 levels showed better survival than those with low Beclin 1 levels (p = 0.009). Beclin 1 protein is upregulated in ovarian epithelial cancer and is a prognostic factor of ovarian cancer.
- Published
- 2014
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228. An Automatic Surface Defect Inspection System for Automobiles Using Machine Vision Methods
- Author
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Qinbang Zhou, Renwen Chen, Bin Huang, Chuan Liu, Jie Yu, and Xiaoqing Yu
- Subjects
flaw detection ,automatic visual inspection ,machine vision ,feature extraction ,support vector machine (SVM) ,Chemical technology ,TP1-1185 - Abstract
Automobile surface defects like scratches or dents occur during the process of manufacturing and cross-border transportation. This will affect consumers’ first impression and the service life of the car itself. In most worldwide automobile industries, the inspection process is mainly performed by human vision, which is unstable and insufficient. The combination of artificial intelligence and the automobile industry shows promise nowadays. However, it is a challenge to inspect such defects in a computer system because of imbalanced illumination, specular highlight reflection, various reflection modes and limited defect features. This paper presents the design and implementation of a novel automatic inspection system (AIS) for automobile surface defects which are the located in or close to style lines, edges and handles. The system consists of image acquisition and image processing devices, operating in a closed environment and noncontact way with four LED light sources. Specifically, we use five plane-array Charge Coupled Device (CCD) cameras to collect images of the five sides of the automobile synchronously. Then the AIS extracts candidate defect regions from the vehicle body image by a multi-scale Hessian matrix fusion method. Finally, candidate defect regions are classified into pseudo-defects, dents and scratches by feature extraction (shape, size, statistics and divergence features) and a support vector machine algorithm. Experimental results demonstrate that automatic inspection system can effectively reduce false detection of pseudo-defects produced by image noise and achieve accuracies of 95.6% in dent defects and 97.1% in scratch defects, which is suitable for customs inspection of imported vehicles.
- Published
- 2019
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229. Single Image Super-Resolution Based on Global Dense Feature Fusion Convolutional Network
- Author
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Wang Xu, Renwen Chen, Bin Huang, Xiang Zhang, and Chuan Liu
- Subjects
dense feature fusion ,convolutional neural network ,image super-resolution ,Chemical technology ,TP1-1185 - Abstract
Deep neural networks (DNNs) have been widely adopted in single image super-resolution (SISR) recently with great success. As a network goes deeper, intermediate features become hierarchical. However, most SISR methods based on DNNs do not make full use of the hierarchical features. The features cannot be read directly by the subsequent layers, therefore, the previous hierarchical information has little influence on the subsequent layer output, and the performance is relatively poor. To address this issue, a novel global dense feature fusion convolutional network (DFFNet) is proposed, which can take full advantage of global intermediate features. Especially, a feature fusion block (FFblock) is introduced as the basic module. Each block can directly read raw global features from previous ones and then learns the feature spatial correlation and channel correlation between features in a holistic way, leading to a continuous global information memory mechanism. Experiments on the benchmark tests show that the proposed method DFFNet achieves favorable performance against the state-of-art methods.
- Published
- 2019
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230. Elevated Levels of Lewis Y and Integrin α5β1 Correlate with Chemotherapeutic Drug Resistance in Epithelial Ovarian Carcinoma
- Author
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Shulan Zhang, Dawo Liu, Beibei Li, Juanjuan Liu, Chuan Liu, Rui Hou, Jian Gao, Song Gao, Zhenhua Hu, and Bei Lin
- Subjects
integrins ,Lewis y antigen ,ovarian caricinoma ,chemoresistance  ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Objective: To measure Lewis y and integrin α5β1 expression in epithelial ovarian carcinoma and to correlate the levels of these molecules with ovarian carcinoma chemotherapy and prognosis. Methods: The study population included 34 ovarian carcinoma patients with chemotherapeutic drug-resistance, six partially drug-sensitive cases, and 52 drug-sensitive cases (92 total). Immunochemistry was used to determine expression of Lewis y antigen and integrin α5β1 in ovarian carcinoma tissues, and correlation of these molecules with chemotherapy resistance was further investigated, Multi-factor logistic regression analysis was applied to investigate: age, surgical stage, grade, subtype of patient cases, metastasis of lymph nodes, residual tumor size, expression levels of Lewis y antigen and integrin α5β1 correlation with ovarian carcinoma chemotherapy resistance. Results: The expression rates of Lewis y antigen and integrins α5 and β1 were significantly greater in the drug-resistant group (91.17%, 85.29%, 88.24%) than the partially sensitive (50.00%, 33.33%, 50.00%) or sensitive groups (61.54%, 57.69%, 55.77%). Binary logistic regression analysis revealed that surgical stage, residual tumor size, and expression of integrin α5 and Lewis y in ovarian carcinoma tissues were independent risk factors for chemotherapeutic drug resistance. Conclusions: Overexpression of Lewis y and integrin α5 are strong risk factors for chemotherapeutic drug resistance in ovarian carcinoma patients.
- Published
- 2012
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231. HMGB1 Induces Secretion of Matrix Vesicles by Macrophages to Enhance Ectopic Mineralization.
- Author
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Qiang Chen, Jun-Jie Bei, Chuan Liu, Shi-Bin Feng, Wei-Bo Zhao, Zhou Zhou, Zheng-Ping Yu, Xiao-Jun Du, and Hou-Yuan Hu
- Subjects
Medicine ,Science - Abstract
Numerous clinical conditions have been linked to ectopic mineralization (EM). This process of pathological biomineralization is complex and not fully elucidated, but thought to be started within matrix vesicles (MVs). We hypothesized that high mobility group box 1 (HMGB1), a cytokine associated with biomineralizing process under physiological and pathological conditions, induces EM via promoting MVs secretion from macrophages. In this study, we found that HMGB1 significantly promoted secretion of MVs from macrophages and subsequently led to mineral deposition in elevated Ca/Pi medium in vitro. Transmission electron microscopy of calcifying MVs showed formation of hydroxyapatite crystals in the vesicle interior. Subcutaneous injection into mice with MVs derived from HMGB1-treated cells showed a greater potential to initiate regional mineralization. Mechanistic experiments revealed that HMGB1 activated neutral sphingomyelinase2 (nSMase2) that involved the receptor for advanced glycation end products (RAGE) and p38 MAPK (upstream of nSMase2). Inhibition of nSMase2 with GW4869 or p38 MAPK with SB-239063 prevented MVs secretion and mineral deposition. Collectively, HMGB1 induces MVs secretion from macrophages at least in part, via the RAGE/p38 MAPK/nSMase2 signaling pathway. Our findings thus reveal a novel mechanism by which HMGB1 induces ectopic mineralization.
- Published
- 2016
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232. Extremely Low-Frequency Electromagnetic Fields Promote In Vitro Neuronal Differentiation and Neurite Outgrowth of Embryonic Neural Stem Cells via Up-Regulating TRPC1.
- Author
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Qinlong Ma, Chunhai Chen, Ping Deng, Gang Zhu, Min Lin, Lei Zhang, Shangcheng Xu, Mindi He, Yonghui Lu, Weixia Duan, Huifeng Pi, Zhengwang Cao, Liping Pei, Min Li, Chuan Liu, Yanwen Zhang, Min Zhong, Zhou Zhou, and Zhengping Yu
- Subjects
Medicine ,Science - Abstract
Exposure to extremely low-frequency electromagnetic fields (ELF-EMFs) can enhance hippocampal neurogenesis in adult mice. However, little is focused on the effects of ELF-EMFs on embryonic neurogenesis. Here, we studied the potential effects of ELF-EMFs on embryonic neural stem cells (eNSCs). We exposed eNSCs to ELF-EMF (50 Hz, 1 mT) for 1, 2, and 3 days with 4 hours per day. We found that eNSC proliferation and maintenance were significantly enhanced after ELF-EMF exposure in proliferation medium. ELF-EMF exposure increased the ratio of differentiated neurons and promoted the neurite outgrowth of eNSC-derived neurons without influencing astrocyes differentiation and the cell apoptosis. In addition, the expression of the proneural genes, NeuroD and Ngn1, which are crucial for neuronal differentiation and neurite outgrowth, was increased after ELF-EMF exposure. Moreover, the expression of transient receptor potential canonical 1 (TRPC1) was significantly up-regulated accompanied by increased the peak amplitude of intracellular calcium level induced by ELF-EMF. Furthermore, silencing TRPC1 expression eliminated the up-regulation of the proneural genes and the promotion of neuronal differentiation and neurite outgrowth induced by ELF-EMF. These results suggest that ELF-EMF exposure promotes the neuronal differentiation and neurite outgrowth of eNSCs via up-regulation the expression of TRPC1 and proneural genes (NeuroD and Ngn1). These findings also provide new insights in understanding the effects of ELF-EMF exposure on embryonic brain development.
- Published
- 2016
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233. ADRC-SMC-based disturbance rejection depth-tracking control of underactuated AUV.
- Author
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Chuan Liu, Xianbo Xiang, Yu Duan, Lichun Yang, and Shaolong Yang
- Published
- 2024
- Full Text
- View/download PDF
234. Graph Convolutional Network Aided Inverse Graph Partitioning for Resource Allocation.
- Author
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Jingwei Wang 0001, Chuan Liu 0001, Yukai Zhao, Zhirui Zhao, Yunlong Ma, Min Liu 0002, and Weiming Shen 0001
- Published
- 2024
- Full Text
- View/download PDF
235. Lewis (y) Antigen Overexpression Increases the Expression of MMP-2 and MMP-9 and Invasion of Human Ovarian Cancer Cells
- Author
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Shulan Zhang, Masao Iwamori, Changzhi Wang, Yifei Wang, Chuan Liu, Song Gao, Lili Gao, Bei Lin, and Limei Yan
- Subjects
Lewis (y) antigen ,matrix metalloproteinases ,tissue inhibitors of metalloproteinases ,invasion ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Lewis (y) antigen is a difucosylated oligosaccharide present on the plasma membrane, and its overexpression is frequently found in human cancers and has been shown to be associated with poor prognosis. Our previous studies have shown that Lewis (y) antigen plays a positive role in the process of invasion and metastasis of ovarian cancer cells. However, the mechanisms by which Lewis (y) antigen enhances the invasion and tumor metastasis are still unknown. In this study, we established a stable cell line constitutively expressing Lewis (y) antigen (RMG-1-hFUT) by transfecting the cDNA encoding part of the human α1,2-fucosyltransferase (α1,2-FUT) gene into the ovarian cancer cell line RMG-1, and investigated whether Lewis (y) antigen regulates the expression of matrix metalloproteinase-2 (MMP-2) and MMP-9, and tissue inhibitors of metalloproteinases (TIMP-1) and TIMP-2. We found that RMG-1-hFUT cells exhibited higher invasive capacities than their control cells. In addition, expression of TIMP-1 and TIMP-2 was down-regulated and expression of MMP-2 and MMP-9 was up-regulated. Anti-Lewis (y) antigen antibody treatment significantly reversed the expression of TIMP-1, TIMP-2, MMP-2 and MMP-9. Taken together, we provide the first evidence that down-regulation of TIMP-1 and TIMP-2 and up-regulation of MMP-2 and MMP-9 represents one of the mechanisms by which Lewis (y) antigen promotes cell invasion.
- Published
- 2010
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236. Experimental Investigation on the Residual Stresses in a Thick Joint with a Partial Repair Weld Using Multiple-Cut Contour Method
- Author
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Chuan Liu, Chunjing Wang, Xiaohua Cheng, Yi Yan, Jiawei Yang, and Yuhang Guo
- Subjects
repair weld ,residual stress ,multiple-cut contour method ,thick welded specimen ,superposition ,Technology ,Electrical engineering. Electronics. Nuclear engineering ,TK1-9971 ,Engineering (General). Civil engineering (General) ,TA1-2040 ,Microscopy ,QH201-278.5 ,Descriptive and experimental mechanics ,QC120-168.85 - Abstract
The stress distributions in a thick welded specimen with a partial repair weld were measured with the three-cut contour method. The longitudinal stress maps in the original weld and the repair weld were obtained and the transverse stress map at the weld centerline in the original weld was acquired. The difference between the longitudinal stress in the partial repair weld and that in the original weld was investigated. Results show that the longitudinal stress increases significantly within the entire repair region with a peak tensile longitudinal stress close to the yield strength of weld material; and the longitudinal stress in the region above the repair weld decreases distinctly after repair; the introduction of the partial repair weld does not affect the stress distribution trend in the original weld (whether it is beyond or above the repair weld), and it has a slight effect on the tensile stress distribution width in the repair region.
- Published
- 2018
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237. Electron spin resonance study of puff-resolved free radical formation in mainstream cigarette smoke
- Author
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Mariana Ghosh, Chuan Liu, and Petre Ionita
- Subjects
Organic chemistry ,QD241-441 - Published
- 2009
- Full Text
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238. Comparative Transcriptome Analysis of Recessive Male Sterility (RGMS) in Sterile and Fertile Brassica napus Lines.
- Author
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Cunmin Qu, Fuyou Fu, Miao Liu, Huiyan Zhao, Chuan Liu, Jiana Li, Zhanglin Tang, Xinfu Xu, Xiao Qiu, Rui Wang, and Kun Lu
- Subjects
Medicine ,Science - Abstract
The recessive genetic male sterility (RGMS) system plays a key role in the production of hybrid varieties in self-pollinating B. napus plants, and prevents negative cytoplasmic effects. However, the complete molecular mechanism of the male sterility during male-gametogenesis in RGMS remains to be determined. To identify transcriptomic changes that occur during the transition to male sterility in RGMS, we examined the male sterile line WSLA and male fertile line WSLB, which are near-isogenic lines (NILs) differing only in the fertility trait. We evaluated the phenotypic features and sterility stage using anatomical analysis. Comparative RNA sequencing analysis revealed that 3,199 genes were differentially expressed between WSLA and WSLB. Many of these genes are mainly involved in biological processes related to flowering, including pollen tube development and growth, pollen wall assembly and modification, and pollen exine formation and pollination. The transcript profiles of 93 genes associated with pollen wall and anther development were determined by quantitative RT-PCR in different flower parts, and classified into the following three major clades: (1) up-regulated in WSLA plants; (2) down-regulated in WSLA plants; and 3) down-regulated in buds, but have a higher expression in stigmas of WSLA than in WSLB. A subset of genes associated with sporopollenin accumulation were all up-regulated in WSLA. An excess of sporopollenin results in defective pollen wall formation, which leads to male sterility in WSLA. Some of the genes identified in this study are candidates for future research, as they could provide important insight into the molecular mechanisms underlying RGMS in WSLA.
- Published
- 2015
- Full Text
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239. Extending Power of Nature from Binary to Real-Valued Graph Learning in Real World.
- Author
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Chunshu Wu, Ruibing Song, Chuan Liu 0001, Yunan Yang, Ang Li 0006, Michael C. Huang 0001, and Tong Geng
- Published
- 2024
240. Extremely low-frequency electromagnetic fields affect transcript levels of neuronal differentiation-related genes in embryonic neural stem cells.
- Author
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Qinlong Ma, Ping Deng, Gang Zhu, Chuan Liu, Lei Zhang, Zhou Zhou, Xue Luo, Min Li, Min Zhong, Zhengping Yu, Chunhai Chen, and Yanwen Zhang
- Subjects
Medicine ,Science - Abstract
Previous studies have reported that extremely low-frequency electromagnetic fields (ELF-EMF) can affect the processes of brain development, but the underlying mechanism is largely unknown. The proliferation and differentiation of embryonic neural stem cells (eNSCs) is essential for brain development during the gestation period. To date, there is no report about the effects of ELF-EMF on eNSCs. In this paper, we studied the effects of ELF-EMF on the proliferation and differentiation of eNSCs. Primary cultured eNSCs were treated with 50 Hz ELF-EMF; various magnetic intensities and exposure times were applied. Our data showed that there was no significant change in cell proliferation, which was evaluated by cell viability (CCK-8 assay), DNA synthesis (Edu incorporation), average diameter of neurospheres, cell cycle distribution (flow cytometry) and transcript levels of cell cycle related genes (P53, P21 and GADD45 detected by real-time PCR). When eNSCs were induced to differentiation, real-time PCR results showed a down-regulation of Sox2 and up-regulation of Math1, Math3, Ngn1 and Tuj1 mRNA levels after 50 Hz ELF-EMF exposure (2 mT for 3 days), but the percentages of neurons (Tuj1 positive cells) and astrocytes (GFAP positive cells) were not altered when detected by immunofluorescence assay. Although cell proliferation and the percentages of neurons and astrocytes differentiated from eNSCs were not affected by 50 Hz ELF-EMF, the expression of genes regulating neuronal differentiation was altered. In conclusion, our results support that 50 Hz ELF-EMF induce molecular changes during eNSCs differentiation, which might be compensated by post-transcriptional mechanisms to support cellular homeostasis.
- Published
- 2014
- Full Text
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241. Plaque bacterial microbiome diversity in children younger than 30 months with or without caries prior to eruption of second primary molars.
- Author
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He Xu, Wenjing Hao, Qiong Zhou, Wenhong Wang, Zhongkui Xia, Chuan Liu, Xiaochi Chen, Man Qin, and Feng Chen
- Subjects
Medicine ,Science - Abstract
OBJECTIVE: Our primary objective is to phylogenetically characterize the supragingival plaque bacterial microbiome of children prior to eruption of second primary molars by pyrosequencing method for studying etiology of early childhood caries. METHODS: Supragingival plaque samples were collected from 10 caries children and 9 caries-free children. Plaque DNA was extracted, used to generate DNA amplicons of the V1-V3 hypervariable region of the bacterial 16S rRNA gene, and subjected to 454-pyrosequencing. RESULTS: On average, over 22,000 sequences per sample were generated. High bacterial diversity was noted in the plaque of children with caries [170 operational taxonomical units (OTU) at 3% divergence] and caries-free children (201 OTU at 3% divergence) with no significant difference. A total of 8 phyla, 15 classes, 21 orders, 30 families, 41 genera and 99 species were represented. In addition, five predominant phyla (Firmicute, Fusobacteria, Proteobacteria, Bacteroidetes and Actinobacteria) and seven genera (Leptotrichia, Streptococcus, Actinomyces, Prevotella, Porphyromonas, Neisseria, and Veillonella) constituted a majority of contents of the total microbiota, independent of the presence or absence of caries. Principal Component Analysis (PCA) presented that caries-related genera included Streptococcus and Veillonella; while Leptotrichia, Selenomonas, Fusobacterium, Capnocytophaga and Porphyromonas were more related to the caries-free samples. Neisseria and Prevotella presented approximately in between. In both groups, the degree of shared organism lineages (as defined by species-level OTUs) among individual supragingival plaque microbiomes was minimal. CONCLUSION: Our study represented for the first time using pyrosequencing to elucidate and monitor supragingival plaque bacterial diversity at such young age with second primary molar unerrupted. Distinctions were revealed between caries and caries-free microbiomes in terms of microbial community structure. We observed differences in abundance for several microbial groups between the caries and caries-free host populations, which were consistent with the ecological plaque hypothesis. Our approach and findings could be extended to correlating microbiomic changes after occlusion establishment and caries treatment.
- Published
- 2014
- Full Text
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242. Differential pro-inflammatory responses of astrocytes and microglia involve STAT3 activation in response to 1800 MHz radiofrequency fields.
- Author
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Yonghui Lu, Mindi He, Yang Zhang, Shangcheng Xu, Lei Zhang, Yue He, Chunhai Chen, Chuan Liu, Huifeng Pi, Zhengping Yu, and Zhou Zhou
- Subjects
Medicine ,Science - Abstract
Microglia and astrocytes play important role in maintaining the homeostasis of central nervous system (CNS). Several CNS impacts have been postulated to be associated with radiofrequency (RF) electromagnetic fields exposure. Given the important role of inflammation in neural physiopathologic processes, we investigated the pro-inflammatory responses of microglia and astrocytes and the involved mechanism in response to RF fields. Microglial N9 and astroglial C8-D1A cells were exposed to 1800 MHz RF for different time with or without pretreatment with STAT3 inhibitor. Microglia and astrocytes were activated by RF exposure indicated by up-regulated CD11b and glial fibrillary acidic protein (GFAP). However, RF exposure induced differential pro-inflammatory responses in astrocytes and microglia, characterized by different expression and release profiles of IL-1β, TNF-α, IL-6, PGE2, nitric oxide (NO), inducible nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX2). Moreover, the RF exposure activated STAT3 in microglia but not in astrocytes. Furthermore, the STAT3 inhibitor Stattic ameliorated the RF-induced release of pro-inflammatory cytokines in microglia but not in astrocytes. Our results demonstrated that RF exposure differentially induced pro-inflammatory responses in microglia and astrocytes, which involved differential activation of STAT3 in microglia and astrocytes. Our data provide novel insights into the potential mechanisms of the reported CNS impacts associated with mobile phone use and present STAT3 as a promising target to protect humans against increasing RF exposure.
- Published
- 2014
- Full Text
- View/download PDF
243. Patterning solution-processed organic single-crystal transistors with high device performance
- Author
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Yun Li, Chuan Liu, Akichika Kumatani, Peter Darmawan, Takeo Minari, and Kazuhito Tsukagoshi
- Subjects
Physics ,QC1-999 - Abstract
We report on the patterning of organic single-crystal transistors with high device performance fabricated via a solution process under ambient conditions. The semiconductor was patterned on substrates via surface selective deposition. Subsequently, solvent-vapor annealing was performed to reorganize the semiconductor into single crystals. The transistors exhibited field-effect mobility (μFET) of up to 3.5 cm2/V s. Good reliability under bias-stress conditions indicates low density of intrinsic defects in crystals and low density of traps at the active interfaces. Furthermore, the Y function method clearly suggests that the variation of μFET of organic crystal transistors was caused by contact resistance. Further improvement of the device with higher μFET with smaller variation can be expected when lower and more uniform contact resistance is achieved.
- Published
- 2011
- Full Text
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244. Use of Classical Adsorption Theory to Understand the Dynamic Filtration of Volatile Toxicants in Cigarette Smoke by Active Carbons
- Author
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Peter J. Branton, Kevin G. McAdam, Martin G. Duke, Chuan Liu, Maria Curle, Michele Mola, Christopher J. Proctor, and Robert H. Bradley
- Subjects
Physical and theoretical chemistry ,QD450-801 - Abstract
The ability of two very different active carbons, a polymer-derived carbon (with ultramicropores and supermicropores, and a large volume of “transport” pores) and a coconut shell-derived carbon (predominantly ultramicroporous), to reduce the levels of volatile toxicants in cigarette smoke has been measured and compared. The polymer-derived carbon was found to be approximately twice as effective in removing the majority of measured smoke vapour-phase toxicants compared to the coconut shell-derived carbon in three different cigarette formats and with two different smoking regimes. Single-component dynamic breakthrough experiments were conducted with benzene, acrylonitrile and 2-butanone at 298 K for beds of each carbon under dry (0% RH) and wet (60% RH) conditions. Longer breakthrough times were found with the polymer-derived carbon, and breakthrough times recorded under wet conditions were found to be up to 20% shorter than those obtained under dry conditions. Correlations between micropore volume, dynamic adsorption volume and filter bed breakthrough time have been demonstrated.
- Published
- 2011
- Full Text
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245. Inertial Confinement Fusion Forecasting via LLMs.
- Author
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Mingkai Chen, Taowen Wang, James Chenhao Liang, Chuan Liu 0001, Chunshu Wu, Qifan Wang, Ying Nian Wu, Michael Huang, Chuang Ren, Ang Li 0006, Tong Geng, and Dongfang Liu
- Published
- 2024
- Full Text
- View/download PDF
246. QQQ: Quality Quattuor-Bit Quantization for Large Language Models.
- Author
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Ying Zhang, Peng Zhang, Mincong Huang, Jingyang Xiang, Yujie Wang, Chao Wang, Yineng Zhang, Lei Yu, Chuan Liu, and Wei Lin
- Published
- 2024
- Full Text
- View/download PDF
247. Incremental FastPitch: Chunk-based High Quality Text to Speech.
- Author
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Muyang Du, Chuan Liu, and Junjie Lai
- Published
- 2024
- Full Text
- View/download PDF
248. Anonymous federated learning framework in the internet of things.
- Author
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Ruizhong Du, Chuan Liu, and Yan Gao
- Published
- 2024
- Full Text
- View/download PDF
249. HMNNet: research on exposure-based nighttime semantic segmentation.
- Author
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Yang Yang, Changjiang Liu, Hao Li, and Chuan Liu
- Published
- 2024
- Full Text
- View/download PDF
250. The protective antibodies induced by a novel epitope of human TNF-alpha could suppress the development of collagen-induced arthritis.
- Author
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Jie Dong, Yaping Gao, Yu Liu, Jinxia Shi, Jiannan Feng, Zhanguo Li, Heping Pan, Yanning Xue, Chuan Liu, Beifen Shen, Ningsheng Shao, and Guang Yang
- Subjects
Medicine ,Science - Abstract
Tumor necrosis factor alpha (TNF-alpha) is a major inflammatory mediator that exhibits actions leading to tissue destruction and hampering recovery from damage. At present, two antibodies against human TNF-alpha (hTNF-alpha) are available, which are widely used for the clinic treatment of certain inflammatory diseases. This work was undertaken to identify a novel functional epitope of hTNF-alpha. We performed screening peptide library against anti-hTNF-alpha antibodies, ELISA and competitive ELISA to obtain the epitope of hTNF-alpha. The key residues of the epitope were identified by means of combinatorial alanine scanning and site-specific mutagenesis. The N terminus (80-91 aa) of hTNF-alpha proved to be a novel epitope (YG1). The two amino acids of YG1, proline and valine, were identified as the key residues, which were important for hTNF-alpha biological function. Furthermore, the function of the epitope was addressed on an animal model of collagen-induced arthritis (CIA). CIA could be suppressed in an animal model by prevaccination with the derivative peptides of YG1. The antibodies of YG1 could also inhibit the cytotoxicity of hTNF-alpha. These results demonstrate that YG1 is a novel epitope associated with the biological function of hTNF-alpha and the antibodies against YG1 can inhibit the development of CIA in animal model, so it would be a potential target of new therapeutic antibodies.
- Published
- 2010
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