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Although shown to be a potent stimulator of serum antibody responses in animal models, the adjuvant immuno-stimulating complexes (ISCOMs) showed little adjuvant effect for inactivated influenza vaccines in a volunteer study. The result may be the non-comparability of the studies: animal studies were carried out chiefly in unprimed mice, while volunteers are mostly primed by previous infection and/or immunization. To test this, Balb/C mice were infected with influenza viruses or immunized with inactivated influenza vaccine, and subsequently given inactivated vaccine in saline or incorporated into ISCOMs. The serum in antibody responses was measured 1 month after immunization. The results confirm the adjuvant activity of ISCOM in unprimed mice, and show a marked reduction in adjuvant activity for primed mice. We argue that ISCOMs are important to prime the T cell response necessary for the serum antibody response to saline vaccine, but largely unnecessary where priming has been accomplished by prior exposure to influenza antigens. Further, the value of ISCOMs may lie in promoting antibody responses in unprimed subjects, and not in enhancing antibody titres.

OVERVIEW OF THE BROOKIAN STRUCTURAL PLAYS THERMAL CONSIDERATIONS STRUCTURAL CLOSURE ANALYSIS BROOKIAN TOPSET STRUCTURAL PLAY PLAY ATTRIBUTES Volumetric Parameters Reservoir thickness Area of closure Porosity and hydrocarbon pore volume Trap Fill Trap depth Number of prospects Oil and Gas parameters Oil recovery factor Non-associated gas recovery factor Oil gravity and sulfur content GOR at median depth Probability of occurrence Play Prospect Charge Trap Timing Aggregate Risking Parameters Fraction of accumulations being oil RESULTS TOROK STRUCTURAL PLAY PLAY ATTRIBUTES Volumetric Parameters Reservoir thickness Area of closure Porosity and hydrocarbon pore volume Trap Fill Trap depth Number of prospects

Introduction Overview Structural Geology the Brooks Range Foothills In The Vicinity of NPRA New Integrated Structural-Stratigraphic Model Thermal Evolution Structural Plays in the Deformed Belt of NPRA THRUST-BELT PLAY Introduction

The global redox switch between aerobic and anaerobic growth in Rhodobacter sphaeroides is controlled by the RegA/RegB two-component system, in which RegB is the integral membrane histidine protein kinase, and RegA is the cytosolic response regulator. Despite the global regulatory importance of this system and its many homologues, there have been no reported examples to date of heterologous expression of full-length RegB or any histidine protein kinases. Here, we report the amplified expression of full-length functional His-tagged RegB in Escherichia coli, its purification, and characterisation of its properties. Both the membrane-bound and purified solubilised RegB protein demonstrate autophosphorylation activity, and the purified protein autophosphorylates at the same rate under both aerobic and anaerobic conditions confirming that an additional regulator is required to control/inhibit autophosphorylation. The intact protein has similar activity to previously characterised soluble forms, but is dephosphorylated more rapidly than the soluble form (half-life ca 30 minutes) demonstrating that the transmembrane segment present in the full-length RegB may be an important regulator of RegB activity. Phosphotransfer from RegB to RegA (overexpressed and purified from E. coli) by RegB is very rapid, as has been reported for the soluble domain. Dephosphorylation of active RegA by full-length RegB has a rate similar to that observed previously for soluble RegB.

The locations of principal faults and structural zones that may influence ground-water flow were compiled in support of a three-dimensional ground-water model for the Death Valley regional flow system, which covers 80,000 km2 in southwestern Nevada and southeastern California. Faults include Neogene extensional and strike-slip faults and pre-Tertiary thrust faults. Emphasis was given to characteristics of faults and deformed zones that may have a high potential for influencing hydraulic conductivity. These include: (1) faulting that results in the juxtaposition of stratigraphic units with contrasting hydrologic properties, which may cause ground-water discharge and other perturbations in the flow system; (2) special physical characteristics of the fault zones, such as brecciation and fracturing, that may cause specific parts of the zone to act either as conduits or as barriers to fluid flow; (3) the presence of a variety of lithologies whose physical and deformational characteristics m ay serve to impede or enhance flow in fault zones; (4) orientation of a fault with respect to the present-day stress field, possibly influencing hydraulic conductivity along the fault zone; and (5) faults that have been active in late Pleistocene or Holocene time and areas of contemporary seismicity, which may be associated with enhanced permeabilities.

[1] The analysis of a global data set of monthly leaf area index (LAI), derived from satellite observations of normalized difference vegetation index (NDVI) for the period July 1981 to September 1994, is discussed in this paper. Validation of this retroactive, coarse resolution (8 km) global multiyear data set is a challenging task because repetitive ground measurements from all representative vegetation types are not available. Therefore the magnitudes and interannual variations in the derived LAI fields were assessed as follows. First, the use of a NDVI-based algorithm, as opposed to a more physically based approach, is estimated to result in relative errors in LAI of about 10–20%, which is comparable to the mean uncertainty of AVHRR NDVI data. Second, the satellite LAI values compared reasonably well to ground measurements from three field campaigns. Third, comparison with an existing multiyear LAI data set showed qualitative agreement with regards to interannual variability, although the LAI values of the earlier data were consistently larger than those derived here. Fourth, interannual variations in LAI were evaluated through correlations with climate data sets, e.g., sea surface temperatures and precipitation in tropical semiarid regions known for ENSO impacts, temperature dependence of vegetation growth, and therefore LAI, in the northern latitudes. The general consistency between these independent data sets imbues confidence in the LAI data set, at least for use in large-scale modeling studies. Finally, improvements in near-surface climate simulation are documented in a companion article when satellite LAI values were used in a global climate model. The data set is available to the community via our Web server (http://cybele.bu.edu).

The development of improved vaccines against epidemic and pandemic influenza virus infection remains a priority in vaccine research. Killed vaccines given by injection are both cost-effective and induce immunity; however, their limitations are well known. Live vaccines have been in development for many years, but difficulties and safety concerns have prohibited their licensing in Western countries. However, the newer technologies of vaccine development, including DNA vaccines and attenuated virus vaccines produced by reverse genetics, remain a hope for the future. With these problems in mind, emphasis has been given to the development of inactivated vaccines that are administered intranasally, either as repeated doses of saline vaccine or in conjunction with suitable carriers or adjuvants. This review describes these latter developments and concludes that this approach offers advantages and should be vigorously researched.

Yucca Mountain, Nye County, Nevada, has been identified as a potential site for underground storage of high-level radioactive nuclear waste. Detailed bedrock geologic maps form an integral part of the site characterization program by providing the fundamental framework for research into the geologic hazards and hydrologic behavior of the mountain. This bedrock geologic map provides the geologic framework and structural setting for the area in and adjacent to the site of the potential repository. The study area comprises the northern and central parts of Yucca Mountain, located on the southern flank of the Timber Mountain-Oasis Valley caldera complex, which was the source for many of the volcanic units in the area. The Timber Mountain-Oasis Valley caldera complex is part of the Miocene southwestern Nevada volcanic field, which is within the Walker Lane belt. This tectonic belt is a northwest-striking megastructure lying between the more active Inyo-Mono and Basin-and-Range subsections o f the southwestern Great Basin.

Bedrock geologic maps form the foundation for investigations that characterize and assess the viability of the potential high-level radioactive waste repository at Yucca Mountain, Nevada. As such, this map focuses on the central block at Yucca Mountain, which contains the potential repository site. The central block is a structural block of Tertiary volcanic rocks bound on the west by the Solitario Canyon Fault, on the east by the Bow Ridge Fault, to the north by the northwest-striking Drill Hole Wash Fault, and on the south by Abandoned Wash. Earlier reconnaissance mapping by Lipman and McKay (1965) provided an overview of the structural setting of Yucca Mountain and formed the foundation for selecting Yucca Mountain as a site for further investigation. They delineated the main block-bounding faults and some of the intrablock faults and outlined the zoned compositional nature of the tuff units that underlie Yucca Mountain. Scott and Bonk (1984) provided a detailed reconnaissance geologic map of favorable area at Yucca Mountain in which to conduct further site-characterization studies. Of their many contributions, they presented a detailed stratigraphy for the volcanic units, defined several other block-bounding faults, and outlined numerous intrablock faults. This study was funded by the U.S. Department of Energy Yucca Mountain Project to provide a detailed (1:6,000-scale) bedrock geologic map for the area within and adjacent to the potential repository area at Yucca Mountain, Nye County, Nevada. Prior to this study, the 1:12,000-scale map of Scott and Bonk (1984) was the primary source of bedrock geologic data for the Yucca Mountain Project. However, targeted detailed mapping within the central block at Yucca Mountain revealed structural complexities along some of the intrablock faults that were not evident at 1:12,000 (Scott and Bonk, 1984). As a result, this study was undertaken to define the character and extent of the dominant structural features in the vicinity of the potential repository. In addition to structural considerations, ongoing subsurface excavation and geologic mapping within the Exploratory Studies Facility (ESF), development of a three-dimensional-framework geologic model, and borehole investigations required use of a consistent stratigraphic system to facilitate surface to underground comparisons. The map units depicted in this report correspond as closely as possible to the proposed stratigraphic nomenclature by Buesch and others (1996), as described.

This report describes the detailed geologic mapping (1:2,400 scale) that was performed in the northern part of the potential nuclear waste repository area at Yucca Mountain, Nevada, to determine the nature and extent of the Sundance Fault zone and to evaluate structural relations between the Sundance and other faults.

Summary Respiratory tract infections are among the commonest of illnesses, and most individuals will experience two to five infections during each year of their lives. The illnesses vary from relatively mild common colds caused by rhinoviruses and coronaviruses, to severe bronchiolitis and pneumonia caused by adenoviruses and influenza viruses and respiratory syncytial virus (RSV) in infants: the former is associated with little morbidity and no mortality, while influenza is responsible annually for between 1 and 25 thousand deaths per 50 million population. Over 140 viruses cause respiratory tract infections, with the added complications of influenza viruses where new antigenic variants are recognized almost annually; and immunity to infection by one virus strain offers little or no protection to infection by others. Knowledge of the mechanisms of spread of respiratory viruses is largely understood and has helped in infection control; however, the clinical signs and symptoms of infection tend not to be diagnostic of the causative agent; and although vaccines have been developed for the more serious infections such as influenza and some adenovirus infection, none are available for other important infections. Treatment is largely symptomatic, but the compounds ribovirin for RSV infection and amantadine for influenza virus infection have been shown to be effective. Much remains to be discovered before more effective measures can be implemented to limit the enormous costs incurred by these infections. The number of viruses involved is large, and the spectrum of illness complex: in the present chapter, the viruses are described, together with the features of the epidemiology, pathogenesis, clinical disease, and treatment.

As part of the Site Characterization effort for the US Department of Energy`s Yucca Mountain Project, tunnels excavated by tunnel boring machines provide access to the volume of rock that is under consideration for possible underground storage of high-level nuclear waste beneath Yucca Mountain, Nevada. The Exploratory Studies Facility, a 7.8-km-long, 7.6-m-diameter tunnel, has been excavated, and a 2.8-km-long, 5-m-diameter Cross Drift will be excavated in 1998 as part of the geologic, hydrologic and geotechnical evaluation of the potential repository. The southwest-trending Cross Drift branches off of the north ramp of the horseshoe-shaped Exploratory Studies Facility. This report summarizes an interpretive geologic section that was prepared for the Yucca Mountain Project as a tool for use in the design and construction of the Cross Drift.

Following the demonstration of the strong adjuvant effect of whole-cell DTP vaccine (wDTP) on the immune responses to influenza subunit vaccine, studies were undertaken to identify the component of wDTP responsible for the adjuvant effect, and to determine if acellular DTP (aDTP) vaccine was as effective since it is less reactogenic and likely to replace wDTP for primary or secondary immunisation. In addition, wDTP and aDTP were directly compared in a dose-response study. Experiments in mice indicated that the adjuvant effect of wDTP resided in the LPS component of B. pertussis , since purified LPS enhanced the IgG antibody response, the IgG subclass response and protection to the same level as wDTP. An adjuvant effect was detected using aDTP, but was statistically less pronounced than wDTP by a factor of some 100-fold. These results suggest that immunisation against influenza in infants and young children can be achieved by combining small amounts of influenza antigen with wDTP or LPS, and to a lesser extent by combining vaccine with aDTP. However, these results were obtained in mice and should be confirmed in man since species vary considerably in response to adjuvant.

Ql Introduction 2 Geologic Setting 2 Data Acquisition and Processing 4 Correlation of Reflections with Regional Stratigraphy 4 Velocity Assumptions 5 Interpretation of the Seismic Profile 6 Stephens Well and Vicinity 6 Tabb Fault System 6 Fluorspar Area Fault Complex 7 Ohio River-Rock Creek Graben Area 10 Discussion 13 Reelfoot-Rough Creek Rift 13 Fluorspar Area Fault Complex 16 Hicks Dome 16 Conclusions 17 References Cited 18

The US Geological Survey has recently purchased over 600 km of multifold seismic reflection profiles in eastern Nevada in the vicinity of Railroad and White River Valleys to understand the regional structural setting of the Railroad Valley oil fields (35 million barrels produced through 1992). One profile extends west of Railroad Valley through the Pancake Range and into Big Sand Springs Valley; another line extends east of White River Valley into Cave, Muleshoe, and Lake valleys. Analysis of sonic logs from over 50 wells indicate that Miocene and younger valley-fill deposits vary from less than 7,000 ft/sec, average velocity in the center of Railroad Valley, to more than 16,000 ft/sec,/near the mountain fronts where conglomerates composed of Paleowic carbonate clasts occur. The conglomerates above and east of the Grand Canyon oil field (18 million barrels as of 1992) appear to have the highest velocities, and these may be due to cementation effects caused by hydrothermal upwelling. Normal faults are generally too steep to be seen as fault-plane reflections (>40{degree}), except in the vicinity of the prolific Grand Canyon oil field, where a low-angle normal fault (approx. 20{degree}) can be seen on two profiles. the overall extension in the study areamore » appears to be in the range of 25--50% except beneath eastern Railroad Valley and the adjacent Grand Range where 80 to more than 100% extension probably has occurred.« less

Unprimed mice and mice primed by prior infection with an H1N1 subtype of influenza virus were used to assess the total and subclass IgG serum antibody responses to influenza virus A/Sichuan/2/87 (H3N2) surface haemagglutinin and neuraminidase proteins incorporated into four different formulations of liposomes. Only one of these liposome preparations, DSPC(B), induced greater total IgG, and subclass IgG1 and IgG2a antibody levels, in sera from both primed and unprimed mice than the aqueous A/Sichuan surface preparations alone administered at equivalent levels of haemagglutinin protein. The same DSPC(B) liposome formulation of A/Sichuan antigens was also the only preparation found to elicit levels of IgG2b and IgG3 subclass antibodies above baseline values in these animals.

DNA ploidy and the measurement of proliferation or S-phase fraction are both of prognostic significance in breast cancer, yet clinical use is minimal in the U.K. Immunohistochemistry is, however, used to aid diagnosis, so a panel of antibodies were analysed by flow cytometry to assess their predictive value for prognosis, tumour stage and grade. Of 10 parameters tested on 202 breast tumour samples, tumour cell proliferation and DNA ploidy were the two most informative; cytokeratin staining, natural killer and B-cell infiltration also proved to be of value but there was no prognostic value in measuring tumour infiltrating monocytes, helper/suppressor T-cell ratios, tumour cell reactivity with carcinoembryonic antigen or human milk fat globulin antibodies. For each of the informative parameters, scores numerically weighted towards a poorer prognosis were derived which when combined, correlated with tumour grade, stage and prognosis. Such data interpretation is objective, and can be transposed to other human tumours.

Specimens from 11 patients presenting with acute epididymitis were tested for the presence of Chlamydia trachomatis by an enzyme immunoassay (EIA), growth in McCoy cells and the polymerase chain reaction (PCR), and for other microorganisms by standard laboratory techniques. Chlamydia trachomatis urethral infection was detected in four patients by tissue culture, in three patients by EIA and in nine patients by PCR. These findings confirm the usually low detection rate of Chlamydia trachomatis by conventional tissue culture and EIA. Detection by PCR indicated both the diagnostic value of this technique and the importance of this organism in epididymitis.

Comparative studies on the local IgA, and circulating IgG subclass antibody responses of mice to A/Sichuan/2/87 (H3N2) influenza virus surface antigens administered with different carrier or delivery systems by the parenteral route, were carried out. The results obtained were compared with the responses observed following live influenza virus infection, and the protection afforded to these animals by these various preparations determined. Infection with live virus elicited early and high levels of protection against homologous virus challenge and this correlated with both local IgA and circulating IgG2a antibody levels. When incorporated into immunostimulating complexes (ISCOMS), A/Sichuan surface antigens promoted high levels of local IgA and circulating IgG1 antibody, and achieved a more rapid and more solid immunity against homologous virus challenge infection, than that elicited by the same surface antigens administered alone or together with Freund's complete adjuvant or alhydrogel.

From abstract: Seismic reflection data from the southern part of the Uinta basin near Price, Utah reveal a network of late Paleozoic faults that produced abrupt variations in stratigraphic thicknesses in a structurally complex 30-mi-wide northwest-southeast-trending trough.

COCORP and petroleum industry seismic reflection profiles in northwestern Montana reveal the structure of the Cordilleran foreland thrust belt. The Front Ranges consist of thick thrust sheets containing Precambrian Belt Supergroup and Paleozoic miogeoclinal shelf rocks above a thin remnant of Paleozoic rocks and gently westward-dipping North American basement. Interpretation of the seismic data and results from a recent petroleum exploration well suggest that 15-22 km of Precambrian Belt Supergroup sedimentary rocks are present in several thrust plates beneath the eastern Purcell anticlinorium. Previous hypotheses of a large mass of Paleozoic miogeoclinal sedimentary rocks or slices of crystalline basement located beneath the eastern Purcell anticlinorium do not appear to be supported by the data. The easternmost occurrence of allochthonous basement is interpreted to be in the western part of the anticlinorium near the Montana-Idaho border. Comparison of the Cordilleran foreland thrust belt in northwestern Montana and southern Canada suggests that a change in the deep structure of the Purcell anticlinorium occurs along strike. The anticlinorium in southern Canada has been interpreted as a hanging-wall anticline that was thrust over the western edge of thick Proterozoic North American basement, whereas in northwestern Montana the anticlinorium appears to consist of a complex series of thrust sheets above highly attenuated North American basement.

Two seismic reflection lines that compose a 90-km east-west profile at approximately 38{degree}25{prime}N latitude, east-central Nevada, help define the structure beneath Railroad Valley, White River Valley, the southern Egan Range, Cave Valley, Muleshoe Valley, the southern Schell Creek Range, and Lake Valley, Preliminary seismic interpretations are being integrated with ongoing geologic mapping, gravity, and magnetic studies and with drill-hole data along this transect. In the Grant Canyon oil field of Railroad Valley, a gently west-dipping normal fault appears to have controlled the development of the Neogene basin. The fault is clearly defined by fault-plane reflections and by terminations of east-dipping reflections from Tertiary and Paleozoic strata that have rotated toward the fault; the fault projects to nearby outcrops of a major low-angle extensional fault mapped in the Grant Range to the east. White River Valley at this latitude consists of three east-dipping half-grabens and two intervening basement highs. Two half-grabens in the western part of the valley are bounded by west-dipping faults with intermediate to steep dips. East-dipping reflections in the southern Egan Range correspond to a homoclinal Paleozoic panel overlain by a veneer of Late Cretaceous and early Tertiary rocks. The north end of Muleshoe Valley yields a narrowmore » sag basin pattern between the southern Schell Creek Range and Dutch John Mountain, with no well-defined bounding faults. Lake Valley, on the east end of the profile, is a broad, complex basin containing normal faults with opposing dips. The progressive steepening of westerly dips in basin-fill beneath the west side of the basin suggests the presence of a major east-dipping listric fault.« less

Cytogenetic analysis is presented on seven freshly derived colorectal tumors and four established cell lines (SW 742, SW 480, SW 948, and HT 29). No chromosome change was common to all tumors, although previous nonrandom findings were confirmed. Single chromosome abnormalities were identified in two cases, 47,XX,+i(7p) and 46,XX,−17, +der(17),t(17;?)(p;?), and their relevance to tumor origin and development is discussed. The association of i(8q) with tumors of the rectosigmoidal region is confirmed, and it is suggested that other rearrangements involving loss of 8p may have the same association. Abnormalities resulting in loss of 20p and duplication of 20q, not previously reported as a nonrandom change, were seen in seven out of 11 cases.

Geologic mapping and fracture studies have documented the fundamental patterns of joints and faults in the thick sequence of rhyolite tuffs at Yucca Mountain, Nevada, the proposed site of an underground repository for high-level radioactive waste. The largest structures are north-striking, block-bounding normal faults (with a subordinate left-lateral component) that divide the mountain into numerous 1–4-km-wide panels of gently east-dipping strata. Block-bounding faults, which underwent Quaternary movement as well as earlier Neogene movement, are linked by dominantly northwest-striking relay faults, especially in the more extended southern part of Yucca Mountain. Intrablock faults are commonly short and discontinuous, except those on the more intensely deformed margins of the blocks. Lithologic properties of the local tuff stratigraphy strongly control the mesoscale fracture network, and locally the fracture network has a strong influence on the nature of intrablock faulting. The least faulted part of Yucca Mountain is the north-central part, the site of the proposed repository. Although bounded by complex normal-fault systems, the 4-km-wide central block contains only sparse intrablock faults. Locally intense jointing appears to be strata-bound. The complexity of deformation and the magnitude of extension increase in all directions away from the proposed repository volume, especially in the southern part of the mountain where the intensity of deformation and the amount of vertical-axis rotation increase markedly. Block-bounding faults were active at Yucca Mountain during and after eruption of the 12.8–12.7 Ma Paintbrush Group, and significant motion on these faults postdated the 11.6 Ma Rainier Mesa Tuff. Diminished fault activity continued into Quaternary time. Roughly half of the stratal tilting in the site area occurred after 11.6 Ma, probably synchronous with the main pulse of vertical-axis rotation, which occurred between 11.6 and 11.45 Ma. Studies of sequential formation of tectonic joints, in the context of regional paleo-stress studies, indicate that north- and northwest-striking joint sets formed coevally with the main faulting episode during regional east-northeast–west-southwest extension and that a prominent northeast-striking joint set formed later, probably after 9 Ma. These structural analyses contribute to the understanding of several important issues at Yucca Mountain, including potential hydrologic pathways, seismic hazards, and fault-displacement hazards.

With an abundance of ice recently detected just below the surface of Mars [Boynton et al., 2002],the possibility that life has existed or still may exist on Mars may hinge on examining its past climate and the duration of surface water flows. Was Mars ever a warm and wet planet, or has it always been cold and dry? The study of surface depressions, in conjunction with river features, yields new insight into these questions. Together, these features provide a more complete picture of a surface water network that may have existed on what must have been a warmer early Mars.

Global land surface characteristics are important boundary conditions for global models that describe exchanges of water, energy, and carbon dioxide between the atmosphere and biosphere. Existing data sets of global land cover are based on classification schemes that characterize each grid cell as a discrete vegetation type. Consequently, parameter fields derived from these data sets are dependent on the particular scheme and the number of vegetation types it includes. The functional controls on exchanges of water, energy, and carbon dioxide between the atmosphere and biosphere are now well enough understood that it is increasingly feasible to model these exchanges using a small number of vegetation characteristics that either are related to or closely related to the functional controls. Ideally, these characteristics would be mapped as continuous distributions to capture mixtures and gradients in vegetation within the cell size of the model. While such an approach makes it more difficult to build models from detailed observations at a small number of sites, it increases the potential for capturing functionally important variation within, as well as between, vegetation types. Globally, the vegetation characteristics that appear to be most important in controlling fluxes of water, energy, and carbon dioxide include (1) growth form (tree, shrub, herb), (2) seasonality of woody vegetation (deciduous, evergreen), (3) leaf type (broadleaf, coniferous), (4) photosynthetic pathway of nonwoody vegetation (C3, C4), (5) longevity (annual, perennial), and (6) type and intensity of disturbance (e.g., cultivation, fire history). Many of these characteristics can be obtained through remote sensing, though some require ground-based information. The minimum number and the identity of the required land surface characteristics almost certainly vary with the intended objective, but the philosophy of driving models with continuous distributions of a small number of land surface characteristics is likely to be applicable to a broad range of problems.

New data from a small enclave that did not undergo significant Neogene tectonism in northwest Utah document Eocene tilting, normal faulting, land folding of the early Eocene lacustrine White Sage Formation. This deformation was part of a complexly deformed Eocene Sevier-Laramide hinterland that underwent east-west contraction and extension in different areas and ultimately gave way to widespread Oligocene extension in the eastern Great Basin. Such “mixed-mode” supracrustal Eocene tectonism may reflect local adjustments to maintain a state of regional gravitational equilibrium, or may record responses to changing plate convergence rates at the west edge of the continent.

Analysis of seismic reflection data reveals that the Seattle basin (Washington) is markedly asymmetric and consists of ∼9-10 km of Eocene and younger deposits. The basin began as a discrete geologic element in the late Eocene (∼40 Ma), the result of a reorganization in regional fault geometry and kinematics. In this reorganization, dextral offset on the Puget fault south- east of Seattle stepped eastward, and the Seattle fault began as a restraining transfer zone. North-vergent reverse or thrust faulting on the Seattle fault forced flexural subsidence in the Seattle basin to the north. Offset on the Seattle fault and subsidence of the Seattle basin have continued to the present.

SUMMARY (1) Incident precipitation, throughfall and stemflow were collected to examine the importance of factors potentially determining net canopy element fluxes, and to quantify canopy exchange and dry deposition rates in a regenerating southern Appalachian forest. (2) Net throughfall fluxes (throughfall minus precipitation transfers) showed consistent canopy effects on rainfall chemistry, with SO42~, PO43~, Cl~, K+, Ca2+ and Mg2+ added to rainfall by foliage, whereas NO3~-N, NH4+-N and H+ ions were absorbed from precipitation. Storm characteristics (event amount and duration) accounted for the largest portion of the variability in growing season net throughfall fluxes, suggesting that canopy exchange was the major mechanism of throughfall transfer. (3) Stemflow fluxes increased canopy exchange rates of SO42~, PO4?~, Cl~, K+, and Mg2+ by greater than 20% in a regression model of total below-canopy element fluxes. (4) Cation leaching fluxes were highly variable (C.V.>50%) over spatial scales of several m2, but could be explained largely by heterogeneity in canopy cover. (5) Foliar cation leaching losses in the early successional forest accounted for 4—13% of leaf nutrient reserves. As cation throughfall transfers were highest during storms with the greatest hydrogen ion uptake from rainwater, it is hypothesized that acid precipitation is causing accelerated foliar nutrient leaching in south-eastern hardwood forests.

Two separate, double-blind studies were carried out in volunteers to compare the reactogenicity of, and serum antibody responses to, whole or ether-Tween-split inactivated influenza virus vaccines. In both studies the ether-Tween-split vaccines induced a lower rate of reactions. The serum hemagglutination-inhibiting (HAI) antibody response of volunteers to the A/Scotland/74 component of the split vaccine used in the first study was significantly greater than that following inoculation of A/ Scotland/74 whole-virus vaccine. The neuraminidase-inhibiting (NI) antibody responses of the volunteers to each vaccine were similar. In the second study, a markedly better NI antibody response to the influenza A virus component was seen following immunization with split-virus vaccine, but the HAI antibody response to both split and whole vaccines was the same. In both studies the serum HAI antibody responses to the B/Hong Kong/73 component of the vaccines were similar. Challenge of the volunteers with attenuated influenza viruses homologous to the influenza A component of the vaccines showed both types of vaccines to be protective.

The effect of Ribavirin on the growth of three transplantable tumours was examined. At a concentration which was non-toxic to CBA mice, the compound reduced significantly the incidence of tumours in mice inoculated with transplanted adenovirus 12 -induced tumours. Ribavirin was toxic for hamsters at a concentration of 100 mg/kg/day; at this concentration the compound reduced the tumour incidence of drug-treated animals inoculated with a transplanted SV40 virus-induced tumour, but the effect was less than that seen in mice. Studies in rats showed that Ribavirin had an inhibitory effect on tumour cell growth; however, drug-treated animals exhibited little or no lymphoreticular response to tumour growth, and this secondary immunosuppressive property of the compound had the collective effect of promoting tumour growth.

The natural killer (NK) cell susceptibility of hamster tumours exhibiting high and low levels of spontaneous metastasis in vivo was investigated. The parent cell line (HSV-2-333-2-26), which was weakly metastatic, and the NK-sensitive target line K562 were both sensitive to hamster natural killer cell activity. In contrast, 2 in vivo cloned sublines, designated met A and met B, exhibited a high level of spontaneous metastasis and were shown to be weakly or non-susceptible to hamster UK reactivity.

An unusual opportunity for special processing of selected COCORP data has provided three-dimensional information on deep crustal structure related to Proterozoic rifting, Mesozoic thrusting, and Cenozoic extension in the interior of the United States Cordillera, near the Okanogan Dome, north-central Washington. Using a set of short, intersecting seismic line segments in conjunction with longer regional east-west and north-south seismic lines, we have found dramatic variation vertically in the orientation of structure throughout the crust in this region. A lower crustal block of south-dipping structures lies above a regionally horizontal Moho found at about 34 km depth.This block is ∼13 km thick and is bounded above by a southwest-dipping structure which is discordant to structures both above and below it and is interpreted as a midcrustal thrust formed during Mesozoic to Paleocene convergence. These relationships suggest that the south- dipping lower crustal features were formed prior to, or during, Mesozoic convergence and may be associated either with Proterozoic rifting of the North American continental margin, earlier Proterozoic basement structure, or with a Mesozoic duplex structure formed along a lateral ramp. A zone of relatively few reflections occurs above the mid-crustal thrust and below a highly reflective upper crust and may represent a separate thrust slice in a basement duplex structure. The inferred duplex is cut by east-dipping reflections that project to surface exposures of mylonites and that may be an Eocene(?) normal fault traceable to mid-crustal depths. This duplex structure beneath the Okanogan Dome is analogous to the proposed crustal-scale duplex beneath the Monashee Deecollement exposed in a doubly plunging antiform 150 km to the north in the Shuswap Complex. The three-dimensional analysis, along with the southerly plunge of the antiform, indicates that a thrust at depth just south of the United States-Canadian border may be continuous with the Monashee Deecollement or with the proposed lower thrusts of the Canadian duplex. If so, then by extending the survey north across the international border, a structure deep in the crust may be traceable to the surface.

The ability of inactivated influenza A vaccines to induce serum HI antibody and immunity to challenge infection was studied in hamsters and in volunteers. Groups of hamsters were immunized with 200 IU of influenza virus A/Scotland/74, A/Port Chalmers/73, A/England/72, or A/Hong Kong/68. The serum HI antibody response of animals to, and immunity to challenge infection was directly related to the known relationship between the vaccine and test viruses. Thus, hamsters given A/Hong Kong/68 or A/England/72 vaccine produced serum HI antibody and immunity to A/Hong Kong virus infection, and animals given A/Scotland/74, A/Port Chalmers/73, and A/England/72 produced antibody and immunity to A/Scotland infection.