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A new multi-antenna transmission scheme, referred to as difference-based spatial modulation (DBSM), is proposed to enhance the reliability of spatial modulation (SM). With the DBSM, a pair of amplitude/phase-shift keying (APSK) symbols are conveyed by each channel-use, but only one of them is radiated from a single transmit antenna. In contrast to conventional SM, DBSM selects the active transmit antenna based on the difference between the two symbols of a channel-use, rather than one of them. The design principle of the APSK constellation in the DBSM is detailed, which guarantees that the mean Euclidean distance between any two DBSM signals mapped by a pair of complementary bit-sequences is the largest possible among all the candidates. In this way, DBSM improves the error performance of conventional SM, even though they have the same achievable data rate given the same system configuration. We demonstrate that channel coding techniques, e.g., convolutional and turbo codes, will enhance the performance gain achieved by DBSM over conventional SM. Both theoretical analysis and simulation results are provided to substantiate the advantages of our proposed DBSM scheme.

Colonoscopy is one of the most effective means of detecting intestinal polyps and colon cancer. With the development of machine vision, doctors often use automation to help diagnose intestinal polyps when using colonoscopy. The high rate of missed polyps during traditional intestinal polyp detection can lead to an increased recurrence rate and cancer of polyps. Therefore, we propose a multi-scale fusion network with boundary judgment function. The network can accurately segment smaller polyps and improve the blurred boundaries of segmented images. The network achieves the purpose of deepening the boundary information by using the attention mechanism by judging the global information generated by the encoder at different levels and the prediction mask map obtained by multi-scale fusion. Experimental results show that the network can accurately identify the boundaries of diminutive polyps and improve the overall accuracy of polyp segmentation. Compared with the latest network models, we achieved first place in several metrics in the mainstream datasets(e.t., Kvasir, CVC-ClinicDB, CVC-300, and ETIS LaribPolypDB). Codes available: https://github.com/Wdy1997/J–Net.git

Time-of-Flight imaging is one of the quintessential techniques in three-dimensional reconstruction. However, depth missing is a common problem in Time-of-Flight imaging, which can be classified into structure-based depth loss and large-area one according to various reasons. Large-area depth loss generally occurs due to close-range overexposure resulting in limited dynamic range in depth sensing. Compared to structure-based depth loss, the recovery of large-area depth missing is more challenging and has been rarely studied. In this paper, a large-area depth recovery framework guided by polarization cues is proposed stemmed from a solid physical basic concerning depth and polarization, to realize high dynamic range in applications. Inspired by RGB-D system and shape-from-polarization technique, a dual camera system is utilized including infrared Time-of-Flight camera and visible polarized camera. A physical model between depth map and polarization cues, specifically depth-gradient and degree-of-polarization, is investigated and established. Based on the physical basics, a corresponding polarization-guided depth recovery algorithm with statistical analysis and image processing approach is introduced. Experimental results towards different targets demonstrate the effectiveness of the proposed method qualitatively and quantitatively, accompanied with outcome analysis and detailed discussions about strengths and future works, which indicates a great potential for the applications of high-quality three-dimensional reconstruction and depth sensing.

The aim of this study was to explore the associations of moderate-to-vigorous-intensity physical activity (MVPA) time and sedentary (SED) time with a history of cardiovascular disease (CVD) and multifactorial (i.e., blood pressure (BP), body mass index (BMI), low-density lipoprotein cholesterol (LDL-C), and glycated hemoglobin A1c (HbA1c)) control status among type 2 diabetes mellitus (T2DM) patients in China. A cross-sectional analysis of 9152 people with type 2 diabetes from the Multifactorial Intervention on Type 2 Diabetes (MIDiab) study was performed. Patients were grouped according to their self-reported MVPA time (low, < 150 min·week−1; moderate, 150 to < 450 min·week−1; high, ≥ 450 min·week−1) and SED time (low, < 4 h·d–1; moderate, 4 to < 8 h·d–1; high, ≥ 8 h·d–1). Participants who self-reported a history of CVD were identified as having a CVD risk. Odds ratios (ORs) and 95% confidence intervals (CIs) of CVD risk and multifactorial control status associated with MVPA time and SED time were estimated using mixed-effect logistic regression models, adjusting for China’s geographical region characteristics. The participants had a mean ± standard deviation (SD) age of (60.87 ± 8.44) years, 44.5% were women, and 25.1% had CVD. After adjustment for potential confounding factors, an inverse association between high MVPA time and CVD risk that was independent of SED time was found, whereas this association was not observed in the moderate-MVPA group. A higher MVPA time was more likely to have a positive effect on the control of BMI. Compared with the reference group (i.e., those with MVPA time ≥ 450 min·week−1 and SED time

Abstract Large-scale urban growth has modified the hydrological cycle of our cities, causing greater and faster runoff. Urban forests (UF), i.e. the stock of trees and shrubs, can substantially reduce runoff; still, how climate, tree functional types influence rainfall partitioning into uptake and runoff is mostly unknown. We analyzed 92 published studies to investigate: interception (I), transpiration (T), soil infiltration (IR) and the subsequent reduction in runoff. Trees showed the best runoff protection compared to other land uses. Within functional types, conifers provided better protection on an annual scale through higher I and T but broadleaved species provided better IR. Regarding tree traits, leaf area index (LAI) showed a positive influence for both I and T. For every unit of LAI increment, additional 5% rainfall partition through T (3%) and I (2%) can be predicted. Overall, runoff was significantly lower under mixed species stands. Increase of conifer stock to 30% in climate zones with significant winter precipitation and to 20% in areas of no dry season can reduce runoff to an additional 4%. The study presented an overview of UF potential to partition rainfall, which might help to select species and land uses in different climate zones for better storm-water management.

Separation of actinides from lanthanides is very important for the safe management of nuclear waste, however still challenging due to the chemical complexity of the f-elements. Here, authors report an efficient strategy with graphene oxide membranes for ion sieving of high valent actinyl ions and spherical lanthanide ions.

Endothelial cells (ECs) play an important role in tumor progression. Currently, the main target of anti-angiogenic therapy is the vascular endothelial growth factor (VEGF) pathway. Some patients do benefit from anti-VEGF/VEGFR therapy; however, a large number of patients do not have response or acquire drug resistance after treatment. Moreover, anti-VEGF/VEGFR therapy may lead to nephrotoxicity and cardiovascular-related side effects due to its action on normal ECs. Therefore, it is necessary to identify targets that are specific to tumor ECs and could be applied to various cancer types. We integrated single-cell RNA sequencing data from six cancer types and constructed a multi-cancer EC atlas to decode the characteristic of tumor ECs. We found that tip-like ECs mainly exist in tumor tissues but barely exist in normal tissues. Tip-like ECs are involved in the promotion of tumor angiogenesis and inhibition on anti-tumor immune responses. Moreover, tumor cells, myeloid cells, and pericytes are the main sources of pro-angiogenic factors. High proportion of tip-like ECs is associated with poor prognosis in multiple cancer types. We also identified that prostate-specific membrane antigen (PSMA) is a specific marker for tip-like ECs in all the cancer types we studied. In summary, we demonstrate that tip-like ECs are the main differential EC subcluster between tumors and normal tissues. Tip-like ECs may promote tumor progression through promoting angiogenesis while inhibiting anti-tumor immune responses. PSMA was a specific marker for tip-like ECs, which could be used as a potential target for the diagnosis and treatment of non-prostate cancers.

In view of the limitations of directional drilling technology and equipment for underground gas drainage in coal mining areas, such as low degree of automation, low drilling ratio of target formation, slow drilling rate of rock formation, and small diameter, the research of rotary geosteering drilling technology and equipment underground coal mine has been carried out, with the core technical problems has overcame such as wireless remote control operation of directional drilling rigs, mechanically automatic drill pipe addition, real-time parameter monitoring and fault diagnosis, coal and rock identification while drilling, and trajectory rotation control, ZDY25000LDK electro-hydraulic controlled directional drilling rig, BLY800/12 mud pump truck with high-pressure and large-volume, YSG(A) Mine geosteering MWD system by dynamic azimuth gamma, ϕ133 mm hydraulic push type rotary steering drilling system and supporting drilling tools have developed, with the maximum torque was 25 000 N·m, the automatically add drill pipe time was less than 55s of the directional drilling rig, the rated flow was 800 L/min of the mud pump truck, the natural gamma measurement error was less than ±5% and the stratum exploration distance was ≥0.5 m of the geosteering MWD system, the applicable speed range was 80−200 r/min and the deflection force was 1.4 t of the rotary steerable drilling system; geosteering drilling technology and rotary steerable drilling technology for near horizontal hole has developed with the technical characteristics and process flow are introduced in detail. The field test was carried out in Tangjiahui Coal Mine in Inner Mongolia. 4 directional drillings were completed, with the maximum drilling depth was 820 m, the total footage was 2 419 m, the directional drilling diameter was 172 mm, the drilling efficiency was increased 25% in coal seam and 30% in rock, which realized the directional drilling from “geometric steering” to “rotary geosteering” underground coal mine, the intelligent level and drilling trajectory quality of underground directional drilling in coal mine has been improved, and provided technical equipment support for efficient underground gas drainage and intelligent mine construction.

The Tibetan Plateau (TP) is characterized by heavily local dust activities, however, the mechanism of interannual variations of winter dust frequency over the TP remain poorly understood. Previous studies showed the autumn Arctic sea ice could significantly influence the winter climate over Eurasia. Whether autumn sea ice affects winter dust activity over the TP or not? Here, we used an integrated surface database to investigate possible mechanisms for interannual variability in the frequency of winter dust events above the TP. This variability, which is thought to be mainly caused by local dust emissions, shows significant correlations with sea ice concentration (SIC) in the Barents and Kara Seas during the preceding autumn. Low Barents–Kara SIC is accompanied by reduced snow depth over northern Eurasia between autumn and winter, which can enhance the Eurasian mid-latitude westerly jet stream. This strengthening increases the cyclogenesis and occurrence of strong surface wind speeds in winter, especially over the TP. In addition, a lower SIC is closely associated with reduced precipitation and snow cover in late autumn and winter over the TP, which in turn enhances warming of the land surface and reduces the area of frozen ground. These anomalies in atmospheric circulation patterns and local surface conditions promote dust events above the TP during winter. The ensemble means of Atmospheric Model Intercomparison Project experiments from Phase 6 of the Coupled Model Inter-comparison Project and the Community Atmosphere Model version 4 can generally reproduce the atmospheric circulation anomalies associated with decreased Barents–Kara SIC. This study reveals the crucial effect that SIC anomalies in the Barents and Kara Seas have on winter dust activities over the TP.

Abstract The study aims to explore a new form of immersive animation scene design and improve the application effect of virtual reality (VR) in immersive animation scene design. First, the research status of VR and the related concepts of animation scene design are analyzed, and the basic characteristics of visual effects in immersive VR animation scenes are summarized. Second, three elements to create immersion in a VR animation scene are color, light and shadow, and digital information technology. Finally, the application of VR technology to an animation scene design is analyzed, the problem of vertigo caused by VR animation scenes is deeply discussed, the vertigo test process in an immersive VR scene is proposed, and an experiment is designed to verify it. The results show that in the design of an immersive VR scene, the relative moving speed of the forward straight line should be maintained less than 6.5 times of the audience’s perceived speed, and the optimal relative moving speed of the scene should be maintained below 5 times of the audience’s perceived speed when they moving backward. In the design of a rotating animation scene, the long-term high-speed rotation should be avoided. If there must be a rotating scene, the rotation speed should be as low as 50°/s. The research provides some guidance for the application of VR to immersive animation scene design. This study discusses immersive animation scene design from dizziness prospective. It is novel. This study introduces relative motion perception illusion and use displacement velocity and relative displacement to analyze VR vertigo. Len motion is divided into forward and reverse linear, horizontal and vertical rotation and camera center rotation to study vertigo source. The dizziness evaluation model is designed and combined with the virtual indoor scene developed to achieve a visual immersion experience.

Abstract Stereo‐digital image correlation (Stereo‐DIC) has been widely explored for modal analysis in plate‐type structures due to its noncontact and full‐field advantages. However, when the traditional stereo‐DIC is adopted to capture the out‐of‐plane displacements, several challenging issues exist such as the development of surface speckles, asynchronous camera recording, and efficiency and accuracy degradation due to high computation costs. Moreover, with the captured out‐of‐plane displacements, effective and efficient evaluation of the high spatial resolution mode shapes and their application to damage localization are also critical problems. To tackle these issues, a speckle‐projection DIC technique using a single high‐speed camera is proposed to obtain the out‐of‐plane vibration displacements. Moreover, an enhanced peak‐picking modal analysis method is adopted to enhance the estimation accuracy and efficiency of mode shapes. In addition, the low‐rank property of mode shapes in an intact state and the spatial sparse property of damage locations are harnessed for the detection of damage positions without requiring reference data on the healthy state. Finally, the modal analysis and damage localization results based on the proposed speckle‐projection DIC are compared with those of the traditional two‐camera stereo‐DIC technique to verify its feasibility and effectiveness. It is found that the differences in the identified resonant frequencies between these two methods are smaller than 1% for higher modes. Moreover, the proposed speckle‐projection DIC has the same accuracy as the traditional two‐camera stereo‐DIC in terms of measurement accuracy, mode shape estimation, and damage localization.

Abstract The ubiquitin-specific peptidase 10 (USP10) plays a context-specific, pro or anti-tumorigenic role in different malignancies. However, the role of USP10 in pancreatic cancer remains unclear. Our protein and RNA level analysis from archived specimens and public databases show that USP10 is overexpressed in pancreatic ductal adenocarcinoma (PDAC) and expression correlates with poor overall patient survival. Phenotypically, silencing USP10 decreased viability, clonal growth and invasive properties of pancreatic cancer cells. Mechanistically, silencing USP10 upregulated BiP and induced endoplasmic reticulum (ER) stress that led to an unfolded protein response (UPR) and upregulation of PERK, IRE1α. Decreased cell viability of USP10 silenced cells could be rescued by a chemical chaperone that promotes protein folding. Our studies suggest that USP10 by protecting pancreatic cancer cells from ER stress may support tumor progression.

Objective The debate on the superiority of single‐ or double‐bundle for anterior cruciate ligament reconstruction has not ceased. The comparative studies on intra‐articular biomechanics after different surgical reconstructions are rare. This study is to evaluate the biomechanical stress distribution intra‐knee after single‐ and double‐bundle anterior cruciate ligament reconstruction by three‐dimensional finite element analysis, and to observe the change of stress concentration under the condition of vertical gradient loads. Methods In this study, magnetic resonance imaging data were extracted from patients and healthy controls for biomechanical analysis. Patients included in the three models were matched in age and sex. The strength and distribution of induced stresses were analyzed in two frequently used procedures, anatomical single‐bundle anterior cruciate ligament reconstruction and anatomical double‐bundle anterior cruciate ligament reconstruction, using femoral‐graft‐tibial system under different loads, to mimic a post‐operation mechanical motion. The three‐dimensional finite‐element models for normal ligament and two surgical methods were applied. A vertical force simulating daily walking was performed on the models to assess the interfacial stresses and displacements of intra‐articular tissues and ligaments. The evaluation results mainly included the stress of each part of ligament and meniscus. The stress values of different parts of three models were extracted and compared. Results The stress of ligament/graft at femoral side of three finite‐element models was significantly higher than at tibial side, while the highest level was observed in single‐bundle reconstruction finite‐element model. With the increase of force, the maximum stress in the medial (7.1–7.1 MPa) and lateral (4.9–7.4 MPa) meniscus of single‐bundle reconstruction finite‐element model shifted from the anterior horn to the central area (p = 0.0161, 0.0479, respectively). The stress was shown to be at a lower level at femoral side and posterior cruciate ligament of intra‐knee in two reconstruction finite‐element models than that in normal finite‐element models, while presented higher level at the tibial side than normal knee (p = 0.3528). The displacement of the femoral side and intra‐knee areas in reconstruction finite‐element models was greater than that in normal finite‐element model (p = 0.0855). Conclusion Compared with the single‐bundle technique, the graft of double‐bundle anterior cruciate ligament reconstruction has better stress dissipation effect and can prevent postoperative meniscus tear more effectively.

Ramie bast is a kind of multipurpose biological material. Selective bio-degradation of ramie bast compounds is an important step to partial utilization of complex polysaccharides. Bacillus subtilis is a kind of dominant species with its ability to degrade ramie bast. In order to understand how polysaccharides were catalyzed and utilized in the metabolism of the bacteria, the proteome of B. subtilis strain 168 was analyzed in the presence of ramie bast as sole carbon source, and the cells were grown in glucose as a reference proteome. In total, 2129 proteins are identified, 1871 proteins were used for quantification between different growth conditions, and 363 proteins were differentially expressed under ramie bast and glucose culturing environment. The carbon source affected the abundance of proteins related to carbohydrate transporters via cell membrane, specific reducing sugars degradation pathway, and central carbon metabolism. qRT-PCR analysis revealed that the mRNA levels of four extracellular enzymes participated in polysaccharides degradation were upregulated under ramie bast condition. This study is the first time to uncover comprehensive information on the proteome response of B. subtilis to ramie bast degradation, and may provide important scientific basis for constructing engineering strains in selective extraction of polysaccharide components in ramie bast.

Mg-Gd-Y-Mn-Sc alloys with different Mn + Sc contents were prepared by hot extrusion and ageing treatment. The effect of Mn + Sc additions on the microstructures, tensile properties, and corresponding strengthening mechanisms of the as-extruded and peak-aged Mg-Gd-Y-Mn-Sc alloys were systematically investigated. Mn + Sc additions had outstanding grain refinement effect on the homogenized alloy and promoted the supersaturation of the Gd and Y atoms in α-Mg matrix, facilitating the dynamic recrystallization (DRX) and dynamic precipitation during hot extrusion. The DRX mainly occurs via discontinuous DRX (D-DRX) and continuous DRX (C-DRX) mechanisms. The as-extruded alloy with higher Mn + Sc contents exhibits better strength-ductility balance with a tensile yield strength of 337 MPa, ultimate tensile strength of 406 MPa and elongation to failure of 10.8%, which is mainly attributed to higher DRX fraction and denser dynamically precipitated particles. The Mn + Sc additions benefits the age-hardening response and notably improves the tensile yield strength to be 484 MPa for the peak-aged alloys by ageing precipitation and GB solute segregation, which is superior than previously reported Mg-RE-Mn alloys.

Societal Impact Statement Combining natural and social science approaches to conduct archeological research on wooden cultural relics is important for exploring major aspects of ancient civilizations. The Forbidden City in Beijing, China, is the largest existing wooden palace complex in the world. We examined ancient DNA of imperial wood “Nanmu” specimens taken from representative structural components of the Forbidden City, in order to provide a new perspective on the long‐standing dispute about its species. This allowed us to accurately identify and properly restore these wooden artifacts and improved our understanding of the past interactions between plant distribution, forest resources, and human activities. Summary Exploring the life styles and production methods of past generations using plant resources can help us to improve our understanding of human civilization. Nanmu, known for its high wood quality, was exclusively used for imperial palace construction in the 15th–19th centuries in China, yet its species has been a subject of long‐standing debate. Here, we revisit this unresolved problem, using morphology and ancient DNA (aDNA) to analyze 21 centuries‐old Nanmu specimens sampled from representative palaces of the Forbidden City. Cytochemical staining demonstrated that endogenous aDNA sporadically occurs in the wood ray parenchyma cells of Nanmu specimens. High‐quality plastid genomes were retrieved from archeological woods for the first time via an aDNA capture method, with 90%–100% coverage (137,663–152,805 bp) and sequence depths of 27.05‐ to 1409.94‐fold. Utilizing these ancient genomes, our results demonstrate that Phoebe zhennan and Phoebe hui are most likely the main species of Nanmu in the Forbidden City. This finding diverges from the prevailing view that Nanmu encompasses woods from the whole genus Phoebe and even its close relative Machilus. It also shows that stringent criteria were used when selecting construction materials for the Forbidden City. By combining morphological traits with aDNA analyses, we provide a new solution for identifying the species of timber used for ancient architecture, and we increase our understanding of the way in which forest resources were recognized and utilized by our ancestors despite the lack of a plant taxonomic framework in ancient times.

Part of a gold deposit belongs to altered type gold deposit. The gold bearing alteration zone is composed of sericite, silicification and pyritization caused by hydrothermal alteration of granite. Most of the ore bodies are hosted in pyrite sericite and some in pyrite sericite granite. Using optical microscope, mineral analysis system (BPMA), scanning electron microscope and energy spectrum, the process mineralogical characteristics of minerals were analyzed. It was found that sulfide ore was the main ore and the depth of oxidation zone was very shallow. The gold grade is 5.23 g/t. The main gold minerals are natural gold and silver gold. The main metal minerals are pyrite, pyrrhotite, galena, chalcopyrite, sphalerite and other sulfide minerals and magnetite and other oxide minerals. Gangue minerals mainly include quartz, sericite, feldspar, etc. The size of gold is fine, all of which are below 37 μm. According to the occurrence state of gold minerals, the content of gold bearing minerals existing in monomer form and coexisting with pyrite is 83.18%, which is easy to recover; the content of gold minerals associated with sericite, quartz and magnetite is 16.82%, which is the main reason for the low gold recovery rate in flotation process.

This study identifies the evolutionarily conserved Exportin 4 as an essential regulator in the nuclear export of circRNAs. Defective circRNA export results in R-loop formation and DNA damage in cells, as well as testis and neurological defects in mice.

Angiogenesis and neurogenesis play irreplaceable roles in bone repair. Although biomaterial implantation that mimics native skeletal tissue is extensively studied, the nerve-vascular network reconstruction is neglected in the design of biomaterials. Our goal here is to establish a periosteum-simulating bilayer hydrogel and explore the efficiency of bone repair via enhancement of angiogenesis and neurogenesis. In this contribution, we designed a bilayer hydrogel platform incorporated with magnesium-ion-modified black phosphorus (BP) nanosheets for promoting neuro-vascularized bone regeneration. Specifically, we incorporated magnesium-ion-modified black phosphorus (BP@Mg) nanosheets into gelatin methacryloyl (GelMA) hydrogel to prepare the upper hydrogel, whereas the bottom hydrogel was designed as a double-network hydrogel system, consisting of two interpenetrating polymer networks composed of GelMA, PEGDA, and β-TCP nanocrystals. The magnesium ion modification process was developed to enhance BP nanosheet stability and provide a sustained release platform for bioactive ions. Our results demonstrated that the upper layer of hydrogel provided a bionic periosteal structure, which significantly facilitated angiogenesis via induction of endothelial cell migration and presented multiple advantages for the upregulation of nerve-related protein expression in neural stem cells (NSCs). Moreover, the bottom layer of the hydrogel significantly promoted bone marrow mesenchymal stem cells (BMSCs) activity and osteogenic differentiation. We next employed the bilayer hydrogel structure to correct rat skull defects. Based on our radiological and histological examinations, the bilayer hydrogel scaffolds markedly enhanced early vascularization and neurogenesis, which prompted eventual bone regeneration and remodeling. Our current strategy paves way for designing nerve-vascular network biomaterials for bone regeneration.

Abstract The high cost of cellulase is one of the main obstacles hindering the large-scale biorefining of lignocellulosic biomass. Therefore, developing efficient method for preparation of cellulase is promising. In the present study, the production of cellulase by Trichoderma reesei, Trichoderma harzianum, and Aspergillus niger was optimized, and the synergistic effect of these cellulase on enzymatic hydrolysis of pretreated ramie stalks was also evaluated. The maximum CMCase (Carboxymethyl Cellulase) and filter paper activity (FPA) produced by T. reesei reached to 3.12 IU/mL and 0.13 IU/mL, respectively. The maximum activities of CMCase (3.68 IU/mL), FPA (0.04 IU/mL) and β-glucosidase (8.44 IU/mL) were obtained from A. niger. The results also showed that under the premise of the same FPA activity, the contribution of β-glucosidase activity to yield of reducing sugar was greater than that of CMCase. Besides, cellulase produced by T. reesei and A. niger had the best synergistic effect on enzymatic hydrolysis of pretreated ramie stalks. The highest reducing sugars yield (417 mg/g dry substrate) was achieved when enzyme cocktail was prepared at the ratio of 1:1, which was 1.36–3.35 folds higher than that of different single enzymes. The present research has provided a novel method for efficient preparation of enzymes consortium for enzymatic hydrolysis of ramie stalks. Graphical Abstract

Grain growth directly influences the plasticity and strength of Mg alloys. As the grain size decreases from the microscale to the nanoscale, the plasticity of Mg alloys continually increases, whereas the strength first increases and later decreases. These trends are observed because the plastic deformation mechanism changes from dislocation–twinning dominance to grain boundary dominance. In this study, the factors influencing grain growth, such as the temperature of plastic deformation/annealing, second-phase particles and solute atoms, are examined to aid effective control of the grain size. Additionally, the mechanisms of grain growth, typically induced by strain and thermal activation, are clarified. Strain-induced grain boundary migration is attributable to the difference in the strain energy stored in adjacent grains with high-density dislocations. Heat-induced grain boundary migration is driven by the difference in the energy of the grain boundary/subgrain boundary and boundary curvature. Abnormal grain growth can be induced by anisotropy of the strain energy, anisotropy of the grain boundary mobility, depinning of the second phase and high misorientation gradient.

Abstract Particle image velocimetry (PIV) is an essential method in experimental fluid dynamics. In recent years, the development of deep learning‐based methods has inspired new approaches to tackle the PIV problem, which considerably improves the accuracy of PIV. However, the supervised learning of PIV is driven by large volumes of data with ground truth information. Therefore, the authors consider unsupervised PIV methods. There has been some work on unsupervised PIV, but they are not nearly as effective as supervised learning PIV. The authors try to improve the effectiveness and accuracy of unsupervised PIV by adding classical PIV methods and physical constraints. In this paper, the authors propose an unsupervised PIV method combined with the cross‐correlation method and divergence‐free constraint, which obtains better performance than other unsupervised PIV methods. The authors compare some classical PIV methods and some deep learning methods, such as LiteFlowNet, LiteFlowNet‐en, and UnLiteFlowNet with the authors’ model on the synthetic dataset. Besides, the authors contrast the results of LiteFlowNet, UnLiteFlowNet and the authors’ model on experimental particle images. As a result, the authors’ model shows comparable performance with classical PIV methods as well as supervised PIV methods and outperforms the previous unsupervised PIV method in most flow cases.

Structural biology, complemented by biochemistry experiments and RNA-binding assays show that the Gemin5 C-terminal region adopts a decamer architecture. Gemin5 decamerization is essential for its role in regulating mRNA translation.

Mitochondria are in a constant balance of fusion and fission. Excessive fission or deficient fusion leads to mitochondrial fragmentation, causing mitochondrial dysfunction and physiological disorders. How the cell prevents excessive fission of mitochondria is not well understood. Here, we report that the fission yeast AAA-ATPase Yta4, which is the homolog of budding yeast Msp1 responsible for clearing mistargeted tail-anchored (TA) proteins on mitochondria, plays a critical role in preventing excessive mitochondrial fission. The absence of Yta4 leads to mild mitochondrial fragmentation in a Dnm1-dependent manner but severe mitochondrial fragmentation upon induction of mitochondrial depolarization. Overexpression of Yta4 delocalizes the receptor proteins of Dnm1, i.e., Fis1 (a TA protein) and Mdv1 (the bridging protein between Fis1 and Dnm1), from mitochondria and reduces the localization of Dnm1 to mitochondria. The effect of Yta4 overexpression on Fis1 and Mdv1, but not Dnm1, depends on the ATPase and translocase activities of Yta4. Moreover, Yta4 interacts with Dnm1, Mdv1, and Fis1. In addition, Yta4 competes with Dnm1 for binding Mdv1 and decreases the affinity of Dnm1 for GTP and inhibits Dnm1 assembly in vitro. These findings suggest a model, in which Yta4 inhibits mitochondrial fission by inhibiting the function of the mitochondrial divisome composed of Fis1, Mdv1, and Dnm1. Therefore, the present work reveals an uncharacterized molecular mechanism underlying the inhibition of mitochondrial fission.

Despite improvements in modern medical therapies, inflammatory diseases, such as atherosclerosis, diabetes, non-alcoholic fatty liver, chronic kidney diseases, and autoimmune diseases have high incidence rates, still threaten human health, and represent a huge financial burden. N6-methyladenosine (m6A) modification of RNA contributes to the pathogenesis of various diseases. As the most widely discussed m6A methyltransferase, the pathogenic role of METTL3 in inflammatory diseases has become a research hotspot, but there has been no comprehensive review of the topic. Here, we summarize the expression changes, modified target genes, and pathogenesis related to METTL3 in cardiovascular, metabolic, degenerative, immune, and infectious diseases, as well as tumors. In addition to epithelial cells, endothelial cells, and fibroblasts, METTL3 also regulates the function of inflammation-related immune cells, including macrophages, neutrophils, dendritic cells, Th17 cells, and NK cells. Regarding therapeutic applications, METTL3 serves as a target for the treatment of inflammatory diseases with natural plant drug components, such as emodin, cinnamaldehyde, total flavonoids of Abelmoschus manihot, and resveratrol. This review focuses on recent advances in the initiation, development, and therapeutic application of METTL3 in inflammatory diseases. Knowledge of the specific regulatory mechanisms involving METTL3 can help to deepen understanding of inflammatory diseases and lay the foundation for the development of precisely targeted drugs to address inflammatory processes.

The shield machine is clogged frequently when tunneling in cohesive strata. Shield clogging is closely linked to the shear strength exhibited at the clay-metal interface. To investigate the impact of anti-sticking coating technology on the shear strength at the clay-metal interface, a series of direct shear tests were conducted. The obtained test results revealed an initial increase in shear stress at the clay-metal interface as shear displacement increased, eventually reaching a state of stabilization. The shear strength exhibited a gradual increase initially, followed by a significant increase, and eventually reached a plateau with the rise in the consistency index. It was observed that the adhesion between the anti-adhesion coating and clay was relatively weak, but the presence of the anti-adhesion coating effectively reduced the risk of shield clogging.

As a class of short non-coding ribonucleic acids (RNAs), microRNAs (miRNA) regulate gene expression in human cells and are expected to be nucleic acid drugs to regulate and treat a variety of biological processes and diseases. However, the issues with potential materials toxicity, quantity production, poor cellular uptake, and endosomal entrapment limit their further applications in clinical practice. Herein, ZIF-8, a metal-organic framework with noncytotoxic zinc (II) as the metal coordination center, was selected as miRNA delivery vector was used to prepare miR-200c-3p@ZIF-8 in one step by Y-shape microfluidic chip to achieve intracellular release with low toxicity, batch size, and efficient cellular uptake. The obtained miR-200c-3p@ZIF-8 was identified by TEM, particle size analysis, XRD, XPS, and zeta potential. Compared with the traditional hydrothermal method, the encapsulation efficiency of miR-200c-3p@ZIF-8 prepared by the microfluidic method is higher, and the particle size is more uniform and controllable. The experimental results in cellular level verified that the ZIF-8 vectors with low cytotoxicity and high miRNAs loading efficiency could significantly improve cellular uptake and endosomal escape of miRNAs, providing a robust and general strategy for nucleic acid drug delivery. As a model, the prepared miR-200c-3p@ZIF-8 is confirmed to be effective in osteoarthritis treatment.

Background: Antibiotics alter the microbial balance commonly resulting in antibiotic-associated diarrhea (AAD). Probiotics may prevent and treat AAD by providing the gut barrier and restoring the gut microflora. This study will overview the Systematic Reviews (SRs) of probiotics in preventing and treating AAD in children. It will also assess the reporting, methodological, and evidence quality of the included SRs to provide evidence for their clinical practice.Methods: After searching PubMed, Embase, Cochrane Library, CNKI, CBM, VIP, and WanFang Data databases, and finally included SRs of probiotics in the prevention and treatment of AAD in children, which were published before 1 October 2022. The reporting, methodological, and evidence quality of the included SRs were assessed by PRISMA 2020 statement, AMSTAR 2 tool, and GRADE system.Results: A total of 20 SRs were included, and the results of PRISMA 2020 showed that 4 out of 20 SRs with relatively complete reporting, and the others within some reporting deficiencies, with scores ranging from 17 points to 26.5 points; the results of AMSTAR 2 showed that 3 SRs belonged to moderate quality level, 10 SRs belonged to low-quality level and 7 SRs being extremely low-quality level; the results of the GRADE system showed that a total of 47 outcomes were reported for the included SRs, three were high-level evidence quality, 16 were medium-level evidence quality, 24 were low-level evidence quality, and four were extremely low-level evidence quality; the results of the Meta-analysis showed that high doses (5–40 billion CFUs per day) of probiotics had a significant effect in the prevention of AAD, but it is too early to conclude the effectiveness and safety of other probiotic drugs for AAD in children, except for Lacticaseibacillus rhamnosus and Saccharomyces boulardii.Conclusion: Current evidence shows that probiotics effectively prevent and treat AAD in children, and the effect of probiotics on pediatric AAD may be a potential dose-response effect. However, the conclusion should be treated with caution due to deficiencies in the methodological, reporting, and evidence quality of the included SRs. Therefore, the methodological, reporting, and evidence quality of relevant SRs still need further improvement.Systematic Review Registration:https://www.crd.york.ac.uk/prospero/, identifier CRD42022362328

Correct usage and maintenance of the enteral nutrition feeding pump system is always a challenge in nutrition support for patients with colorectal cancer (CRC). However, there are few studies on the sustained accuracy improvement of the enteral nutrition feeding system in discharged CRC patients. Here, we established a seven-month quality control circle (QCC) activity with the theme of improving the performance of home enteral feeding pumps (EFP) and examined the effect of QCC activity on the nutritional state and quality of life in discharged CRC patients. We enrolled 100 discharged CRC patients treated with home enteral nutrition from Zhejiang Cancer Hospital between March 2020 and December 2021. The patients were randomly split into two research groups: one participated in the QCC activity (n = 50) and the other did not (n = 50). QCC analysis indicated that the top 3 causes of EFP inaccurate usage are the simple and boring contents of training, various types of pumps, no examination rules, and lack of management. Furthermore, both intra- and inter-group comparisons showed that QCC significantly improved the patients’ pass rate of nutrition pump operation from 52 to 70% after 1-month of activity, which gradually improved and got the highest (90%) after 3 months (p

RationaleA family of short synthetic, triphosphorylated stem-loop RNAs (SLRs) have been designed to activate the retinoic-acid-inducible gene I (RIG-I) pathway and induce a potent interferon (IFN) response, which may have therapeutic potential. We investigated immune response modulation by SLR10. We addressed whether RIG-I pathway activation with SLR10 leads to protection of nonsmoking (NS) and cigarette smoke (CS)-exposed mice after influenza A virus (IAV) infection.MethodsMice were given 25 µg of SLR10 1 day before IAV infection. We compared the survival rates and host immune responses of NS and CS-exposed mice following challenge with IAV.ResultsSLR10 significantly decreased weight loss and increased survival rates in both NS and CS-exposed mice during IAV infection. SLR10 administration repaired the impaired proinflammatory response in CS-exposed mice without causing more lung injury in NS mice as assessed by physiologic measurements. Although histopathologic study revealed that SLR10 administration was likely to result in higher pathological scores than untreated groups in both NS and CS mice, this change was not enough to increase lung injury evaluated by lung-to-body weight ratio. Both qRT-PCR on lung tissues and multiplex immunoassay on bronchoalveolar lavage fluids (BALFs) showed that most IFNs and proinflammatory cytokines were expressed at lower levels in SLR10-treated NS mice than control-treaded NS mice at day 5 post infection (p.i.). Remarkably, proinflammatory cytokines IL-6, IL-12, and GM-CSF were increased in CS-exposed mice by SLR10 at day 5 p.i. Significantly, SLR10 elevated the ratio of the two chemokines (CXCL9 and CCL17) in BALFs, suggesting macrophages were polarized to classically activated (M1) status. In vitro testing also found that SLR10 not only stimulated human alveolar macrophage polarization to an M1 phenotype, but also reversed cigarette smoke extract (CSE)-induced M2 to M1 polarization.ConclusionsOur data show that SLR10 administration in mice is protective for both NS and CS-exposed IAV-infected mice. Mechanistically, SLR10 treatment promoted M1 macrophage polarization in the lung during influenza infection. The protective effects by SLR10 may be a promising intervention for therapy for infections with viruses, particularly those with CS-enhanced susceptibility to adverse outcomes.

Marigold can protect crops against soil-borne diseases. However, the effects of intercropping with marigold on apple rhizosphere soils are not known. In this study, we investigated the metabolite profiles and bacterial community structures in rhizosphere soils of the apple-marigold intercropping system by high-throughput sequencing and soil metabolomics. The results show that intercropping marigold could significantly enhance soil moisture, nitrogen, and enzyme activities compared with clean tillage. The soil metabolite profiles and the soil bacterial community structures in the rhizosphere soils were different between the inter-and mono-cropping systems. Among nine metabolites, carbohydrates were more increased in the intercropping system than in the monocropping system. Pathway enrichment analysis revealed that the greatest differential, in terms of metabolic pathway, was starch and sucrose metabolism. Moreover, intercropping marigold significantly increased the relative abundance of plant growth promoting bacteria in rhizosphere soils, such as Rhizobiales, Pseudomonadales, and Bacillales. These results indicate that marigold intercropping positively affected the apple orchard’s soil quality and may provide a new intercropping technique to improve soil fertility in orchards and promote plant growth.

Abstract Generally, long wavelength absorbed near‐infrared II (NIR‐II) dyes have a low fluorescence efficiency in aggregate states for aggregate‐caused quenching effect, simultaneously enhancing efficiency and extending absorption is a challenging issue for NIR‐II dyes. Here, three benzo[1,2‐c:4,5‐c']bis[1,2,5]thiadiazole (BBT) derivatives (TPA‐BBT, FT‐BBT, and BTBT‐BBT) are used to clarify fluorescence quenching mechanisms. When the BBT derivatives are doped into a small molecule matrix, they show quite different fluorescence behaviors. Structure‐distorted TPA‐BBT displays fluorescence quenching originating from short‐range exchange interaction, while FT‐BBT and BTBT‐BBT with a co‐planar‐conjugated backbone exhibit concentration‐dependent quenching processes, namely changing from long‐range dipole‐dipole interaction to exchange interaction, which could be majorly ascribed to large spectral overlap between absorption and emission. By precisely tuning doping concentration, both FT‐BBT and BTBT‐BBT nanoparticles (NPs) present the optimal NIR‐II fluorescence brightness at ∼2.5 wt% doping concentration. The doped NPs have good biocompatibility and could be served as fluorescence contrast agents for vascular imaging with a high resolution under 980‐nm laser excitation. Those paradigms evidence that molecular doping can promote fluorescence efficiency of long wavelength‐absorbed NIR‐II fluorophores via suppressing long‐range energy migration.

Background and aimsMitogen-activated protein kinases (MAPKs) have been reported to respond to various stimuli including heat stress. This research aimed to investigate whether StMAPK1 is implicated in the transduction of the heat stress signal to adapt heat stress as a thermos-tolerant gene.Materials and methodsPotato plants were cultivated under mild (30°C) and acute (35°C) heat stress conditions to analyze mRNA expression of StMAPKs and physiological indicators. StMAPK1 was up-regulated and down-regulated by transfection. Subcellular localization of StMAPK1 protein was observed by fluorescence microscope. The transgenic potato plants were assayed for physiological indexes, photosynthesis, cellular membrane integrity, and heat stress response gene expression.ResultsHeat stress altered the expression prolife of StMAPKs. StMAPK1 overexpression changed the physiological characteristics and phenotypes of potato plants under heat stresses. StMAPK1 mediates photosynthesis and maintains membrane integrity of potato plants in response to heat stress. Stress response genes (StP5CS, StCAT, StSOD, and StPOD) in potato plants were altered by StMAPK1 dysregulation. mRNA expression of heat stress genes (StHSP90, StHSP70, StHSP20, and StHSFA3) was affected by StMAPK1.ConclusionsStMAPK1 overexpression increases the heat-tolerant capacity of potato plants at the morphological, physiological, molecular, and genetic levels.

ABSTRACT The histone acetyltransferase (HAT) Gcn5 ortholog is essential for a variety of fungi. Here, we characterize the roles of Ada2 and Ada3, which are functionally linked to Gcn5, in the insect-pathogenic fungus Beauveria bassiana. Loss of Ada2 and Ada3 led to severe hyphal growth defects on rich and minimal media and drastic decreases in blastospore yield and conidiation capacity, with abnormal conidia-producing structures. ΔAda2 and ΔAda3 exhibited a delay in conidial germination and increased sensitivity to multiple chemical stresses and heat shock. Nearly all their pathogenicity was lost, and their ability to secrete extracellular enzymes, Pr1 proteases and chitinases for cuticle degradation was reduced. A yeast two-hybrid assay demonstrated that Ada2 binds to Ada3 and directly interacts with Gcn5, confirming the existence of a yeast-like Ada3-Ada2-Gcn5 HAT complex in this fungus. Additionally, deletion of the Ada genes reduced the activity of Gcn5, especially in the ΔAda2 strain, which was consistent with the acetylation level of histone H3 determined by Western blotting. These results illustrate the dependence of Gcn5 enzyme activity on Ada2 and Ada3 in fungal hyphal growth, asexual development, multiple stress responses, and pathogenicity in B. bassiana. IMPORTANCE The histone acetyltransferase Gcn5 ortholog contributes significantly to the growth and development of various fungi. In this study, we found that Ada2 and Ada3 have critical regulatory effects on Gcn5 enzyme activity and influence the acetylation of histone H3. Deletion of Ada2 or Ada3 decreased the fungal growth rate and asexual conidial yield and increased susceptibility to multiple stresses in Beauveria bassiana. Importantly, Ada genes are vital virulence factors, and their deletion caused the most virulence loss, mainly by inhibiting the activity of a series of hydrolytic enzymes and the dimorphic transition ability. These findings provide a new perspective on the function of the Gcn5 acetyltransferase complex in pathogens.

Summary: Photocatalytic microplastics (MPs) conversion into valuable products is a promising approach to alleviate MPs pollution in aquatic environments. Herein, we developed an amorphous alloy/photocatalyst composite (FeB/TiO2) that can successfully convert polystyrene (PS) MPs to clean H2 fuel and valuable organic compounds (92.3% particle size reduction of PS-MPs and 103.5 μmol H2 production in 12 h). FeB effectively enhanced the light-absorption and carrier separation of TiO2, thereby promoting more reactive oxygen species generation (especially ‧OH) and combination of photoelectrons with protons. The main products (e.g., benzaldehyde, benzoic acid, etc.) were identified. Additionally, the dominant PS-MPs photoconversion pathway was elucidated based on density functional theory calculations, by which the significant role of ‧OH was demonstrated in combination with radical quenching data. This study provides a prospective approach to mitigate MPs pollution in aquatic environments and reveals the synergistic mechanism governing the photocatalytic conversion of MPs and generation of H2 fuel.

Urban flooding can seriously threaten urban ecological security and human life, and therefore urban flood resilience (UFR) is important for urban safety and stability. To comprehensively evaluate urban system performance during the entire process of rainfall, runoff, flooding, and drainage, we developed a systematic framework for UFR assessment covering runoff simulation, flood estimation, and resilience assessment, which broadly corresponded to the phases of resistance, adaptation, and recovery. The UFR in the phases of resistance, adaptation, and recovery was simulated and assessed using a system performance curve (SPC) and technically combining with the hybrid flood model while mainly considering the total simulation time and inundated urban proportion in SPC. Because the extent of urban flooding can be influenced by climate change and the rate of urbanization, we chose the corresponding representative factors of precipitation and infiltration rate and considered 21 simulation scenarios (seven rainfall return periods and three infiltration rates) for which UFR was quantified according to urban system performance. The effectiveness of this framework was demonstrated in application to a typical highly urbanized area (i.e., Dongguan, China). The following results were derived: (1) The inundated area under the pessimistic scenario (i.e., S19) would be nearly four times greater than that under the optimistic scenario (i.e., S3); (2) The values of UFR in Dongguan were 0.9494–0.9863, locating at the high and very high level; (3) The lowest UFR value was 0.6552 in the Shuixiang New City district; and (4) The rainfall return period was the main factor influencing UFR under relatively short rainfall return periods (i.e., S1–S9), while infiltration rate was the principal influencing factor under relatively long rainfall return periods (i.e., S10–S21). The proposed systematic framework could be applied in other cities and large-scale regions like urban agglomerations and provinces.

In the exploration of ocean resources, the submarine electric field signal plays a crucial role through marine electromagnetic methods. However, due to the field signal’s low-frequency and weak characteristics, it often encounters interference from the instrument’s own 1/f noise during its acquisition. To address this issue, we developed a low-noise amplifier for the submarine electric field signal based on chopping amplification technology. This amplifier utilizes low-temperature electronic components to adapt to the cold submarine environment and enhances its independence by incorporating a square wave generator. Additionally, we conducted simulations and experimental tests on the designed chopper amplifier circuit, evaluating the equivalent input voltage noise spectrum (EIVNS) and the frequency response within 1 mHz~100 Hz. The experimental results indicate that the amplifier designed in this study achieves sufficiently low noise 2 nV/√Hz@1 mHz, effectively amplifying the submarine electric field signal measured with the electric field sensor.

It is urgent to develop novel technologies to convert carbon dioxide to graphene. In this work, a bubble-mediated approach via a chemical reaction between carbon dioxide gas and magnesium melt to fabricate a few-layer graphene was illustrated. The morphology and defects of graphene can be regulated by manipulating the melt temperature. The preparation of graphene at 720 °C exhibited an excellent quality of surface and graphitization degree. The high-quality few-layer graphene can be grown under the combined effect of carbon dioxide bubbles and in-situ grown MgO. This preparation method possesses the advantages of high efficiency, low cost, and environmental protection, which may provide a new strategy for the recovery and reuse of greenhouse gases.

Chiral pesticides exhibit enantioselective differences in processes such as biological absorption, metabolism, and toxic effects. Organisms have different physiological characteristics at different developmental stages. Therefore, conducting enantiomeric toxicity studies at different developmental stages of organisms can help deepen the understanding of the ecological effects of chiral pesticides. This study focused on trans-tetramethrin (Tet) and investigated the enantioselectivity in bioconcentration, developmental toxicity, estrogenic effects, and immunotoxicity of Tet’s racemate ((±)-Tet) and its two enantiomers ((+)-Tet and (−)-Tet) in three developmental stages of zebrafish: embryos, yolk sac larvae, and juveniles. The results showed that Tet exhibited different enantioselectivity in lethal, bioconcentration, and teratogenic effects on zebrafish at different developmental stages. The LC50 value was (+)-Tet > (±)-Tet > (−)-Tet, with embryos being the most sensitive, followed by juveniles and yolk sac larvae. The enantioselective bioconcentration was (±)-Tet > (+)-Tet > (−)-Tet, and the bioconcentration effect was greater in embryos than that in yolk sac larvae and juveniles. Developmental toxicity indicated that (+)-Tet and (±)-Tet had higher teratogenic effects on yolk sac larvae than on embryos. Tet exhibited different enantioselective effects on the expression of zebrafish estrogen-related genes and innate immune-related genes at different developmental stages. These results will contribute to a more comprehensive assessment of the aquatic toxicity and environmental risks of chiral pesticides.

In the directed co-graph edge-deletion problem, we are given a directed graph and an integer k, and the question is whether we can delete, at most, k edges so that the resulting graph is a directed co-graph. In this paper, we make two minor contributions. Firstly, we show that the problem is NP-hard. Then, we show that directed co-graphs are fully characterized by eight forbidden structures, each having, at most, six edges. Based on the symmetry properties and several refined observations, we develop a branching algorithm with a running time of O(2.733k), which is significantly more efficient compared to the brute-force algorithm, which has a running time of O(6k).

The raccoon dog (Nyctereutes procyonoides) is a typical omnivore possessing wide dietary adaptability and tolerance to rough feeding, which may be attributed to its intestinal microbiota. The study aimed to investigate the effect of dietary alfalfa meal levels on the growth performance, nutrient apparent digestibility, serum parameters, and intestinal microbiota of raccoon dogs. Sixty raccoon dogs were randomly divided into four dietary treatments containing 0% (AM0), 5% (AM5), 10% (AM10), and 15% (AM15) alfalfa meal for a 60-day experiment. The results showed that compared to raccoon dogs fed the AM0 diet, those fed the AM5 and AM10 diets had no significant difference in growth performance, while those fed the AM15 diet experienced a significant decrease. Raccoon dogs fed the AM5 diet had no significant effect on the nutrient apparent digestibility. Dietary supplementation with alfalfa meal significantly decreased serum urea levels and increased the antioxidant capacity of raccoon dogs. The intestinal microbiome analysis showed that the richness and diversity of colonic microbiota significantly increased in the AM15 group. With the increase in dietary alfalfa meal levels, the relative abundance of fiber-degrading bacteria in the colon of raccoon dogs, such as Treponema, Phascolarctobacterium, and Christensenellaceae R-7 group, increased. However, the relative abundance of pathogenic bacteria, including Anaerobiospirillum, decreased. In conclusion, the inclusion of 5% alfalfa meal in the raccoon dogs’ diet had no effect on growth performance, but it exhibited the potential to improve serum antioxidant capacity and intestinal microbiota. This indicates that raccoon dogs have a certain tolerance to the addition of alfalfa meal in their diet.

Research on the electrical properties of rocks and ores plays a crucial role in the development of geophysical electromagnetism methods. However, currently available instruments suffer from high power consumption, a limited number of electrodes, inaccurate measurements, poor portability, and a limited ability to measure the electrical parameters of rocks and ores. To address these issues, this paper presents a three-dimensional electrical impedance tomography system for rock samples with high-density microelectrodes based on an Android system and STM32 microcontroller. The system features high observation accuracy, dense electrode arrays (with 384 current and potential electrodes), flexible electrode selection, user-friendly human–computer interaction, good stability, and real-time performance. Powered by a single power bank, the entire instrument can be controlled and monitored wirelessly via Bluetooth and Wi-Fi technology using an Android smartphone. Additionally, the system not only enables accurate measurement of electrical parameters, but also facilitates the generation of three-dimensional impedance imaging of specimens via inversion algorithms after data export, allowing for a comprehensive understanding of the electrical properties of rocks and ores. This system holds great potential for future research in this field.

The purpose of this study is to assess effects of two different simulation methods (i.e., interfaces with a single spring-slider system and interfaces with double spring-slider systems) for interactions between reinforcement and the surrounding medium on the performances of geosynthetic-reinforced soil (GRS) structures when conducting numerical analyses. The fundamental difference between these two methods is the number of the spring-slider systems used to connect the nodes of structural elements simulating the geosynthetic reinforcement and the points of solid grids simulating the surrounding medium. Numerical simulation results of pull-out tests show that both methods reasonably predicted the pullout failure mode of the reinforcement embedded in the surrounding medium. However, the method using the interfaces with a single spring-slider system could not correctly predict the interface shear failure mode between the geosynthetics and surrounding medium. Further research shows that these two methods resulted in different predictions of the performance of GRS piers as compared with results of a laboratory load test. Numerical analyses show that a combination of interfaces with double spring-slider systems for reinforcement between facing blocks and interfaces with a single spring-slider system for reinforcement in soil resulted in the best performance prediction of the GRS structures as compared with the test results. This study also proposes and verifies an equivalent method for determining/converting the interface stiffness and strength parameters for these two methods.

The advancement of unmanned platforms is driving the miniaturization and cost reduction of the multi-beam echosounder (MBES). In the process of MBES array calibration, the mutual coupling significantly impacts the performance of parameter estimation. We propose a correction method to mitigate the mutual coupling effects in the calibration of MBES acoustic array. Initially, a near-field focused beamforming model is established to assess the influence of mutual coupling. Subsequently, the covariance matrix in the frequency domain is constructed to enhance algorithm efficiency and simplify solution procedures. This construction eliminates the need for a low-pass filtering step after heterodyning through extracting peak values near zero frequency in the signal frequency domain. Meanwhile, the Toeplitz property is leveraged to render the estimation results independent of the mutual coupling matrix. Finally, the mutual coupling coefficients and the direction of arrival (DOA) are joint-estimated and the Cramér–Rao bound is derived. The presented method effectively addresses the engineering challenge of MBES mutual coupling calibration. Additionally, the performance of the proposed method is verified through the measured data in simulation and tank experiments.

The authors wish to make the following corrections to this paper [...]

Ochratoxin A (OTA) is one of the mycotoxins that poses a serious threat to human and animal health. Curcumin (CUR) is a major bioactive component of turmeric that provides multiple health benefits. CUR can reduce the toxicities induced by mycotoxins, but the underlying molecular mechanisms remain largely unknown. To explore the effects of CUR on OTA toxicity and identify the key regulators and metabolites involved in the biological processes, we performed metabolomic and transcriptomic analyses of livers from OTA-exposed mice. We found that CUR can alleviate the toxic effects of OTA on body growth and liver functions. In addition, CUR supplementation significantly affects the expressions of 1584 genes and 97 metabolites. Integrated analyses of transcriptomic and metabolomic data showed that the pathways including Arachidonic acid metabolism, Purine metabolism, and Cholesterol metabolism were significantly enriched. Pantothenic acid (PA) was identified as a key metabolite, the exogenous supplementation of which was observed to significantly alleviate the OTA-induced accumulation of reactive oxygen species and cell apoptosis. Further mechanistical analyses revealed that PA can downregulate the expression level of proapoptotic protein BAX, enhance the expression level of apoptosis inhibitory protein BCL2, and decrease the level of phosphorylated extracellular signal-regulated kinase 1/2 (pERK1/2). This study demonstrated that CUR can alleviate the adverse effects of OTA by influencing the transcriptomic and metabolomic profiles of livers, which may contribute to the application of CUR in food and feed products for the prevention of OTA toxicity.

Organic anion transporting polypeptide (OATP) 1B1 and OATP1B3 are key determinants of drug–drug interactions (DDIs). Various drugs including the calcineurin inhibitor (CNI) cyclosporine A (CsA) exert preincubation-induced trans-inhibitory effects upon OATP1B1 and/or OATP1B3 (abbreviated as OATP1B1/3) by unknown mechanism(s). OATP1B1/3 are phosphoproteins; calcineurin, which dephosphorylates and regulates numerous phosphoproteins, has not previously been investigated in the context of preincubation-induced trans-inhibition of OATP1B1/3. Herein, we compare the trans-inhibitory effects exerted on OATP1B1 and OATP1B3 by CsA, the non-analogous CNI tacrolimus, and the non-CNI CsA analogue SCY-635 in transporter-overexpressing human embryonic kidney (HEK) 293 stable cell lines. Preincubation (10–60 min) with tacrolimus (1–10 µM) rapidly and significantly reduces OATP1B1- and OATP1B3-mediated transport up to 0.18 ± 0.03- and 0.20 ± 0.02-fold compared to the control, respectively. Both CsA and SCY-635 can trans-inhibit OATP1B1, with the inhibitory effects progressively increasing over a 60 min preincubation time. At each equivalent preincubation time, CsA has greater trans-inhibitory effects toward OATP1B1 than SCY-635. Preincubation with SCY-635 for 60 min yielded IC50 of 2.2 ± 1.4 µM against OATP1B1, which is ~18 fold greater than that of CsA (0.12 ± 0.04 µM). Furthermore, a proteomics-based screening for protein interactors was used to examine possible proteins and processes contributing to OATP1B1/3 regulation and preincubation-induced inhibition by CNIs and other drugs. A total of 861 and 357 proteins were identified as specifically associated with OATP1B1 and OATP1B3, respectively, including various protein kinases, ubiquitin-related enzymes, the tacrolimus (FK506)-binding proteins FKBP5 and FKBP8, and several known regulatory targets of calcineurin. The current study reports several novel findings that expand our understanding of impaired OATP1B1/3 function; these include preincubation-induced trans-inhibition of OATP1B1/3 by the CNI tacrolimus, greater preincubation-induced inhibition by CsA compared to its non-CNI analogue SCY-635, and association of OATP1B1/3 with various proteins relevant to established and candidate OATP1B1/3 regulatory processes.