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Abstract Maize (Zea mays L.) is one of the most widely distributed and important crops in China. Maize ear differentiation plays an important role grain yield formation. However, it is unclear if ear and root morphophysiology status affects yield formation by altering ear differentiation and development under different nitrogen (N) conditions. The aim of this study is to understand how the ear differentiation and development are affected by ear and root morphophysiology traits, as affected by the N rate. The experiment consisted of two N rates: high nitrogen (180 kg ha−1), and low nitrogen (60 kg ha−1). Two N-efficient varieties (NEVs) and two N-inefficient varieties (NIVs) were grown in the field. The results showed higher nitrogen accumulation and grain yield in NEVs than in NIVs, which was mainly attributed to the increased N uptake by the larger root system under both N conditions. Under high N conditions, among ear differentiation-related traits, only FR was significantly positively correlated with grain yield, and NEVs ensure FR through higher N concentration and ZR content in ear at the fertilization stage. Under low N conditions, NEVs obtained higher FP, SR and FR through higher N concentration and IAA in ear at the early stage of ear differentiation, maintained lower AR and BTL by higher RA, R-ZR and E-ZR at the late stage of ear growth. These results suggest that NEVs have a more complex mechanism for obtaining higher grain yield under low N conditions than N sufficiency, and that phytohormones play an important role in this process.

ObjectiveTo explore spatiotemporal distribution of pulmonary tuberculosis (TB) morbidity and mortality and their changing trend in China from 2004 to 2018 and to provide evidence for conducting relevant studies and developing strategies on TB prevention and treatment. MethodsNationwide data of 2004 – 2018 on TB surveillance were collected and demographic data of the same period were extracted from China Statistical Yearbook. The 15-year period was divided into three stages. The spatial characteristics of TB morbidity was analyzed with Excel 2010 software and Stata 14.0 was used to analyze spatial autocorrelation. Results During the period, totally 14 816 329 TB incidents were reported in China, with an average incidence rate of 73.9029/100 000 and an average annual reported mortality of 0.2114/100 000. Spatial autocor-relation of TB morbidity in different regions was observed. ConclusionDuring 2004 – 2018 in China, the incidence rate of pulmonary tuberculosis was decreasing generally and there were aggregations and significant regional differences in TB morbidity.

The key modules of downhole very broadband seismograph include multi-components core machinery and reed system, core feedback adjustment system, and downhole positioning and orientation system.

Abstract As the world continues to experience the COVID-19 pandemic, seasonal influenza remain a cause of severe morbidity and mortality globally. Worse yet, coinfection with SARS-CoV-2 and influenza A virus (IAV) leads to more severe clinical outcomes. The development of a combined vaccine against both COVID-19 and influenza is thus of high priority. Based on our established lipid nanoparticle (LNP)-encapsulated mRNA vaccine platform, we developed and characterized a novel mRNA vaccine encoding the HA antigen of influenza A (H1N1) virus, termed ARIAV. Then, ARIAV was combined with our COVID-19 mRNA vaccine ARCoV, which encodes the receptor-binding domain (RBD) of the SARS-CoV-2 S protein, to formulate the final combined vaccine, AR-CoV/IAV. Further characterization demonstrated that immunization with two doses of AR-CoV/IAV elicited robust protective antibodies as well as antigen-specific cellular immune responses against SARS-CoV-2 and IAV. More importantly, AR-CoV/IAV immunization protected mice from coinfection with IAV and the SARS-CoV-2 Alpha and Delta variants. Our results highlight the potential of the LNP-mRNA vaccine platform in preventing COVID-19 and influenza, as well as other respiratory diseases.

ObjectiveTo explore mediating effect of fear of falling (FoF) on the association between activities of daily living (ADL) and frailty among rural elderly for providing references to frailty prevention in the elderly. MethodsUsing a multistage stratified random sampling method, we conducted a questionnaire survey among 3 600 permanent rural residents aged ≥ 60 years in three counties of Shandong province from May to July 2019. The Frail Scale, 10-Item Kessler Psychological Distress Scale (K10), Mini-Mental State Examination (MMSE), Activities of Daily Living Scale (ADL), and a self-designed questionnaire were adopted in the survey. Statistical analyses were performed on the data collected from 3 242 valid respondents and the RMediation program of R 3.6.1 software was used to evaluate mediating effect of FoF. ResultsFor all the valid respondents, the frailty score was 1.52 ± 1.08, and 18.11% (587), 64.68% (2 097), and 17.21% (558) of the respondents were assessed in the frail, pre-frail, and non-frail condition, respectively. The respondents had an average ADL score of 16.99 ± 4.37, with 87.88% (2 849) and 12.12% (393) having normal and decreased ADL. More than a half of the respondents (58.14%, 1 885) reported FoF. Spearman correlation analysis showed that decreased ADL and FoF were positively associated with frailty (r = 0.265 and r = 0.161, both P < 0.001), and declined ADL was also positively associated with FoF (r = 0.112, P < 0.001). Multivariate logistic regression analysis indicated that decreased ADL could increase the risk of frailty [odds ratio (OR) = 3.06, 95% confidence interval (95% CI): 2.40 – 3.90] and FoF (OR = 1.70, 95% CI: 1.32 – 2.20); FoF was a risk factor for both frailty (OR = 1.37, 95% CI: 1.17 – 1.60) and weakened relationship between ADL and frailty (OR = 2.98, 95% CI: 2.33 – 3.80). The results of mediating effect analysis revealed that FoF played a partial mediation effect on the association between ADL and frailty and the 95% CI was 0.08 – 0.27 for the effect value. ConclusionAmong rural elderly in Shandong province, FoF is a mediator between ADL and frailty and psychological intervention on FoF needs to be promoted among the elderly with decreased ADL for attenuating frail condition of the elderly.

Abstract Immune reactions provoked by cerebral ischemia play crucial roles in the pathogenesis of brain damage and contribute to tissue regeneration processes. While functions of many immune cell types in post-ischemic inflammation have been well studied in experimental stroke, the exact roles played by unconventional T cells in pathogenesis of the clinical stroke remain to be precisely determined. In the present study, we investigated the frequencies and absolute cell numbers of peripheral blood T lymphocyte subpopulations including those of invariant natural killer T (iNKT) cells, CD3+CD56+ NKT-like (NKTL) cells, and γδ T cells from patients with acute cerebral infarction (ACI), chronic cerebrovascular disease (CCD) or chronic cerebral circulation insufficiency (CCI) by flow cytometry, and analyzed their association with the disease severity and the clinical outcome. We observed significantly reduced cell numbers of circulating iNKT cells, NKTL cells and γδ T cells in cerebral ischemia patients as compared with the healthy controls. Of note, we also demonstrated that numbers of peripheral blood iNKT and γδ T cells are significantly reduced in patients with ACI when compared among different cerebral ischemia patient groups. Moreover, the reduced number of iNKT cells is significantly associated with the disease severity and recovery in cerebral ischemia patients. Our results demonstrate for the first time the reduction of peripheral blood NKTL, iNKT and γδ T cells in patients with the cerebral ischemia, and particularly reduced iNKT and γδ T cells in the acute phase. The reduction of iNKT cells seems to be significantly associated with the disease severity and recovery. We hope that our findings might lead to the identification of predictive and prognostic values of human peripheral unconventional T cell subsets in the cerebral ischemia.

Metallocene-based phosphines are compounds with potential use in catalysis. Here, the authors report the electrochemical regioselective functionalization of group 8 metallocenes with phosphine oxides; over 60 examples of phosphorylated (benzo)ferrocenes and ruthenocenes can be accessed via this method without the need for a preinstalled directing group.

Abstract Background Modern sequencing technologies have generated low-cost microbiome survey datasets, across sample sites, conditions, and treatments, on an unprecedented scale and throughput. These datasets often come with a phylogenetic tree that provides a unique opportunity to examine how shared evolutionary history affects the different patterns in host-associated microbial communities. Results In this paper, we describe an R package, phyloMDA, for phylogeny-aware microbiome data analysis. It includes the Dirichlet-tree multinomial model for multivariate abundance data, tree-guided empirical Bayes estimation of microbial compositions, and tree-based multiscale regression methods with relative abundances as predictors. Conclusion phyloMDA is a versatile and user-friendly tool to analyze microbiome data while incorporating the phylogenetic information and addressing some of the challenges posed by the data.

Abstract Background Therapy with genetically modified mesenchymal stem cells (MSCs) has clinical translation promise. Optimizing the targeting migratory ability of MSCs relies on accurate imaging of the distribution and extravasation kinetics of MSCs, and the corresponding imaging results could be used to predict therapeutic outcomes and guide the optimization of the treatment program. Among the different imaging modalities, second near-infrared (NIR-II) optical-resolution photoacoustic microscopy (OR-PAM) has merits, including a fine resolution, a deep penetration, a high sensitivity, and a large signal-to-background ratio. It would be an ideal candidate for precise monitoring of MSCs, although it has not been tested for this purpose so far. Results Penetrating peptide-decorated conjugated polymer nanoparticles (TAT-CPNPs) with strong NIR-II absorbance were used to label chemokine-receptor genetically modified MSCs, which were subsequently evaluated under intravital NIR-II OR-PAM regarding their targeting migratory ability. Based on the upregulation of chemokine (C-X-C motif) ligand 10 in the inflamed ears of contact hypersensitivity mice, MSCs with overexpression of corresponding receptor, chemokine (C-X-C motif) receptor 3 (Cxcr3) were successfully generated (MSCCxcr3). TAT-CPNPs labeling enabled NIR-II photoacoustic imaging to discern MSCCxcr3 covered by 1.2 cm of chicken breast tissue. Longitudinal OR-PAM imaging revealed enhanced inflammation-targeting migration of MSCCxcr3 over time attributed to Cxcr3 gene modification, which was further validated by histological analysis. Conclusions TAT-CPNPs-assisted NIR-II PA imaging is promising for monitoring distribution and extravasation kinetics of MSCs, which would greatly facilitate optimizing MSC-based therapy. Graphical Abstract

The vacuum level in micro-electro-mechanical system devices needs to be achieved and maintained using non-evaporable getter film technology. Zr–Co–RE getter films are deposited by direct current (DC) magnetron sputtering using krypton (Kr) as the sputtering gas. The influence of sputtering gas pressure and DC sputtering power on the microstructure and adsorption characteristics of films is investigated. Zr–Co–RE films deposited at different gas pressures all grow in a columnar shape. Films grown at low Kr pressures are relatively dense and have a structure with fewer cracks, whereas high Kr pressures result in a uniform cluster and columnar structure with more gaps and micro-cracks. In addition, it is revealed that DC sputtering power has great influence on the film structure and adsorption performance. The films deposited at low sputtering power contain more micro-cracks, which are distributed uniformly. At high sputtering power, the cluster structure is not obvious. Consequently, high Kr pressures and low sputtering power are beneficial to improve the adsorption performance. Hydrogen adsorption tests are carried out using a special vacuum system, keeping the pressure constant. The highest initial adsorption speed is 89 (ml/s)/cm2, obtained at 4.0 Pa Kr pressure and 300 W sputtering power. Meanwhile, the Zr–Co–RE films deposited under these conditions have excellent adsorption stability.

The aim of this paper is to compare the static and dynamic performances of two types of herringbone grooved journal bearings (HGJBs), that is, pumping-out HGJB and pumping-in HGJB. Firstly, based on finite control volume method and the principle of flow balance, an equivalent Reynolds equation considering the turbulence effect is derived. Then, the journal motion considering angular displacement is analyzed with linear perturbation method. The equivalent Reynolds equation is linearized into static-form one and perturbed-form ones. These equations are solved by finite difference method and their solutions are integrated on the bearing surface to determinate the static characteristics and dynamic coefficients of HGJB. Additionally, the critical mass and critical whirl frequency are defined for evaluating the stability and rigidity of bearing by solving a motion equation with four degree of freedoms (DOFs). Finally, the static and dynamic characteristics of two types of HGJBS are analyzed and compared symmetrically in consideration of effects of eccentricity ratio, length to diameter ratio, groove angle, groove depth, the width of sealing damp, tilting degree of journal, and rotation speed. The results show that, within the interested range of investigated parameters, static performances of pumping-out HGJB are superior to that of pumping-in HGJB generally. Stability of pumping-in HGJB is better than pumping-out HGJB but bearing rigidity of pumping-in HGJB is inferior to pumping-out HGJB.

An emerging application of nanotechnology in medicine currently being developed involves employing nanoparticles to deliver drugs, heat, light, or other substances to specific types of cells (such as cancer cells). As most biological molecules exist and function at the nanoscale, engineering and manipulating matter at the molecular level has many advantages in the field of medicine (nanomedicine). Although encouraging, it remains unclear how much of this will ultimately result in improved patient care. In surgical specialties, clinically relevant nanotechnology applications include the creation of surgical instruments, suture materials, imaging, targeted drug therapy, visualization methods, and wound healing techniques. Burn lesion and scar management is an essential nanotechnology application. Prevention, diagnosis, and treatment of numerous orthopedic conditions are crucial technological aspects for patients’ functional recovery. Orthopedic surgery is a specialty that deals with the diagnosis and treatment of musculoskeletal disorders. In recent years, the field of orthopedics has been revolutionized by the advent of nanotechnology. Using biomaterials comprised of nanoparticles and structures, it is possible to substantially enhance the efficacy of such interactions through nanoscale material modifications. This serves as the foundation for the majority of orthopedic nanotechnology applications. In orthopedic surgery, nanotechnology has been applied to improve surgical outcomes, enhance bone healing, and reduce complications associated with orthopedic procedures. This mini-review summarizes the present state of nanotechnology in orthopedic surgery, including its applications as well as possible future directions.

BackgroundThe objective was to measure the correlations of preoperative levels of folate receptor-positive circulating tumor cells (FR+CTCs) with clinical characteristics and histologic subtype in early-stage lung adenocarcinoma, and to determine the predictive value of FR+CTC level in preoperative determination of the extent of surgical resection.Patients and methodsIn this retrospective, single-institution, observational study, preoperative FR+CTC levels were measured via ligand-targeted enzyme-linked polymerization in patients with early-stage lung adenocarcinoma. Receiver operating characteristic (ROC) analysis was used to identify the optimal cutoff value of FR+CTC level for prediction of various clinical characteristics and histologic subtypes.ResultsNo significant difference in FR+CTC level was observed among patients with adenocarcinoma in situ (AIS), minimally invasive adenocarcinoma (MIA), and invasive adenocarcinoma (IAC) (P = 0.813). Within the non-mucinous adenocarcinoma group, no difference was observed among patients with tumors whose predominant growth patterns were lepidic, acinar, papillary, micropapillary, solid, and complex gland (P = 0.053). However, significant differences in FR+CTC level were observed between patients with and without the micropapillary subtype [11.21 (8.22-13.61) vs. 9.85 (7.43-12.63), P = 0.017], between those with and without the solid subtype [12.16 (8.27-14.90) vs. 9.87 (7.50-12.49), P = 0.022], and between those with any of the advanced subtypes (micropapillary, solid, or complex glands) vs. none of these [10.48 (7.83-13.67) vs. 9.76 (7.42-12.42), P = 0.032]. FR+CTC level was also correlated with degree of differentiation of lung adenocarcinoma (P = 0.033), presence of visceral pleural invasion (VPI) of lung carcinoma (P = 0.003), and lymph node metastasis of lung carcinoma (P = 0.035).ConclusionFR+CTC level is of potential predictive value in determining the presence of aggressive histologic patterns (micropapillary, solid, and advanced subtypes), degree of differentiation, and occurrence of VPI and lymph node metastasis in IAC. Measurement of FR+CTC level combined with intraoperative frozen sections may represent a more effective method of guiding resection strategy in cases of cT1N0M0 IAC with high-risk factors.

Aberrant mitophagy has been identified as a driver for energy metabolism disorder in most cardiac pathological processes. However, finding effective targeted agents and uncovering their precise modulatory mechanisms remain unconquered. Fuzi, the lateral roots of Aconitum carmichaelii, shows unique efficacy in reviving Yang for resuscitation, which has been widely used in clinics. As a main cardiotonic component of Fuzi, mesaconine has been proven effective in various cardiomyopathy models. Here, we aimed to define a previously unrevealed cardioprotective mechanism of mesaconine-mediated restoration of obstructive mitophagy. The functional implications of mesaconine were evaluated in doxorubicin (DOX)-induced heart failure models. DOX-treated mice showed characteristic cardiac dysfunction, ectopic myocardial energy disorder, and impaired mitophagy in cardiomyocytes, which could be remarkably reversed by mesaconine. The cardioprotective effect of mesaconine was primarily attributed to its ability to promote the restoration of mitophagy in cardiomyocytes, as evidenced by elevated expression of PINK1, a key mediator of mitophagy induction. Silencing PINK1 or deactivating mitophagy could completely abolish the protective effects of mesaconine. Together, our findings suggest that the cardioprotective effects of mesaconine appear to be dependent on the activation of PINK1-induced mitophagy and that mesaconine may constitute a promising therapeutic agent for the treatment of heart failure.

Bredia bullata, B. enchengensis, and B. nitida (Sonerileae, Melastomataceae), three species occurring in Sino-Vietnamese limestone karst regions, are described as new. Molecular phylogenetic analyses and morphological divergence indicate that these species are well separated from their close relatives in Bredia, justifying their recognition as distinct species. Bredia bullata is unique in its interveinal areas prominently bullate each with an apical seta, a character otherwise never recorded in the genus. Bredia nitida resembles B. malipoensis in habit, leaf shape, and inflorescence morphology, but differs in the glabrescent and nitid adaxial leaf surface (vs. densely pubescent and subvelvety), ovate-elliptic or elliptic calyx lobes (vs. triangular to semiorbicular), and white petals (vs. purplish-red). Bredia enchengensis is closest to B. longiradiosa, but easily recognized by its prostrate habit (vs. erect), the yellowish-green, membranous and fragile leaves (vs. green or dark green, papery), and white anthers (vs. pink to purplish). These new discoveries show that further botanical exploration is warranted in the remote Sino-Vietnamese bordering region.

Abstract Background Chemotherapy is a common approach for cancer treatment, but intrinsic genetic mutations in different individuals may cause different responses to chemotherapy, resulting in unique histopathological changes. The genetic mutation along with the distinct histopathological features may indicate new tumor entities. BCOR-CCNB3 sarcomas is a kind of Ewing-like sarcomas (ELS) occurring mostly in bone and soft tissues. No gene fusion other than BCOR-CCNB3 has been found in this type of tumor. Case presentation We herein report a case of 17-year-old male patient, presented with a mass on his left shoulder that was diagnosed as undifferentiated small round cell sarcoma according to core biopsy. The patient received 5 courses of preoperational chemotherapy, and the tumor was resected and analyzed. Primitive small round cells and larger myoid cells in the resected tumor tissue but not in biopsy were observed, and arterioles stenosis and occlusion were also detected, indicating a dramatic change of histopathological features of this tumor. In addition, the immunohistochemical results showed the altered staining patterns of BCOR, bcl2, CyclinD1, TLE1, AR, SMA, CD117, STAB2, CD56, and CD99 in tumor tissues after chemotherapy. Notably, RNA sequencing revealed a RNF213-SLC26A11 fusion in the tumor sample. Conclusions The BCOR-CCNB3 sarcoma with RNF213-SLC26A11 fusion may indicate a subset of tumors that undergo histopathological changes in response to chemotherapy. More similar cases in the future may help to clarify the clinical meanings of RNF213-SLC26A11 fusion in BCOR-CCNB3 sarcomas and the underlying mechanisms.

To ensure the stability of an overlying roadway in close proximity to a 1000-m deep coal seam, this work, through theoretical analysis and numerical simulation, studies the deformation and failure mechanism, failure types, and prevention methods of the overlying roadway while mining a lower coal seam. Based on the study of mining conditions and overburdened space structures formed through mining, the deformation and failure law of the roadway in the strike and dip directions is obtained. Moreover, the stress evolution law of roadway deformation and failure is obtained by studying the influence of different mining stages and filling rates of the lower face on the mining of the overlying roadway. Results show that there are two types of potential roadway deformation and failure: (1) roadway section reduction failure and (2) roadway strike step subsidence failure. The fundamental cause of the deformation and failure of the overlying roadway is the influence of the large buried depth, strong mining stress, and unequal distance between the coal seam and roadway. The direct causes are the sudden increase in the stress of the roadway surrounding the rock and the subsidence of the key strata. In the mining process, when the mining range of the working face is more than 400 m, the roadway section reduction failure and strike ladder subsidence failure superimpose each other, inducing further roadway failure. Therefore, a scheme of partial filling and strengthening of the roadway along the working face is designed. Field measurements revealed that the above scheme can meet the stability requirements of the overlying roadway and the high efficiency and yield of the underlying working face. The results provide some references for the safe mining of coal in the 1000-m deep coal seam.

Abstract With the advent of the 5G era, the design of electronic equipment is developing towards thinness, intelligence and multi-function, which requires higher cooling performance of the equipment. Micro-channel heat sink is promising for the heat dissipation of super-thin electronic equipment. In this study, thermal resistance theoretical model of the micro-channel heat sink was first established. Then, fabrication process of the micro-channel heat sink was introduced. Subsequently, heat transfer performance of the fabricated micro-channel heat sink was tested through the developed testing platform. Results show that the developed micro-channel heat sink has more superior heat dissipation performance over conventional metal solid heat sink and it is well suited for high power LEDs application. Moreover, the micro-channel structures in the heat sink were optimized by orthogonal test. Based on the orthogonal optimization, heat dissipation performance of the micro-channel radiator was further improved.

The safe and stable operation of power transmission and transformation equipment is the foundation of power grid safety, so the prediction of power transmission and transformation equipment fault is particularly important, and the prediction of the characteristic parameters that affect the equipment fault is the core of fault prediction. In this paper, a method for predicting the characteristic parameters of power transmission and transformation equipment based on combination prediction is presented. According to the correlation analysis results of various parameters, the key prediction input data with high correlation degree with the predicted parameters are extracted automatically, and the method library including multiple single-factor prediction models, multi-factor prediction models and combined prediction models is established. The error checking method is used to automatically select the optimal prediction method, and then the characteristic parameters of the parameter are automatically predicted. The test results show that the multi-factor power transmission and transformation equipment fault feature parameter prediction method can improve the accuracy of prediction, and is of great significance to improve the scientific evaluation of equipment health status and the prediction of equipment fault.

X65 carbon steel is widely used in subsea gathering and transportation pipelines. In the gathering and transportation process, the natural gas medium is complex and often contains corrosive gases such as H2S and CO2. Due to the use of wet gas transportation, condensate water was formed, resulting in serious corrosion perforation accidents of pipelines. In this work, through the experiments of high - temperature and high - pressure reactor, the corrosion mechanism of gas phase, liquid phase and gas - liquid interface were analyzed, which provided a basis for the formulation of corrosion prevention and control measures for X65 steel wet gas gathering and transmission pipeline. Moreover, high - temperature and high - pressure reactor experiments were carried out with X65 steel in the presence of CO2, H2S and Cl - respectively. Each experiment was carried out for 7 d for gas - liquid interface corrosion, and the characteristics of corrosion products were analyzed by surface analysis technologies, such as scanning electron microscope (SEM) and X - ray diffraction (XRD). Results showed that with the injection of CO2 and H2S, the local corrosion rate increased in three regions of gas phase, liquid phase, and gas - liquid interface. In the presence of CO2 and H2S, the order of corrosion rate was liquid phase corrosion rate > gas phase corrosion rate > interface corrosion rate. Under the condition of gas - liquid interface, X65 steel formed anode below the interface and cathode above the interface, so that the local corrosion rate of liquid phase was faster.

Glioblastoma (GBM) is the deadliest primary brain tumor and is generally resistant to immunotherapy because of severe dysfunction of T cells. Novel treatment options are critically needed to overcome the immunotherapy resistance of GBM. Here we demonstrate that Zika virus (ZIKV) treatment improves the efficacy of anti-PD ligand 1 (PD-L1) immunotherapy in GBM. We found that ZIKV induces a strong pro-inflammatory response and increases CD4+ and CD8+ T cell intratumoral infiltration and activation in GBM mouse models. ZIKV treatment of mice bearing GBM tumors inhibits tumor growth and prolongs survival. These therapeutic effects of ZIKV on GBM tumors are negated in mice depleted of T cells. Moreover, ZIKV dramatically promotes activation of the type I interferon signaling pathway in GBM cells. ZIKV treatment potently sensitizes GBM to PD-L1 blockade and provides significant and durable survival benefits. Our findings reveal that ZIKV overcomes the resistance of GBM to immune checkpoint blockade, which may lead to therapeutic applications of ZIKV in individuals with GBM receiving immunotherapy.

Human Enterovirus 71 (EV71) has emerged as one of the predominant causative agents of hand, foot and mouth disease (HFMD) with global impact. Despite the inactivated vaccine being licensed, other vaccine candidates based on advanced technology platforms are under development. In this report, we rationally designed and constructed two DNA-launched live attenuated vaccine candidates (pDL-EV71) under the control of specific promoters. In vitro and in vivo transfection with pDL-EV71 driven by the CMV promoter successfully yielded fully infectious EV71. More importantly, the administration of pDL-EV71 did not cause clinical symptoms following intracranial or intramuscular inoculation in neonatal and IFNα/βR-/- mice, demonstrating its safety profile. Moreover, a single-dose or two-dose immunization with pDL-EV71 elicited robust neutralizing antibodies against EV71 as well as an antigen-specific cellular response in mice. A single-dose immunization with 10 μg of pDL-EV71 conferred complete protection against lethal EV71 infection in neonates born to immunized maternal mice. Overall, our present results demonstrate that pDL-EV71 is a safe and effective vaccine candidate against EV71 for further development. [ABSTRACT FROM AUTHOR]

Landslide susceptibility mapping (LSM) is significant for landslide risk assessment. However, there remains no consensus on which method is optimal for LSM. This study implements a dynamic approach to landslide hazard mapping by integrating spatio-temporal probability analysis with time-varying ground deformation velocity derived from the MT-InSAR (Multi-Temporal InSAR) method. Reliable landslide susceptibility maps (LSMs) can inform landslide risk managers and government officials. First, sixteen factors were selected to construct a causal factor system for LSM. Next, Pearson correlation analysis, multicollinearity analysis, information gain ratio, and GeoDetector methods were applied to remove the least important factors of STI, plan curvature, TRI, and slope length. Subsequently, information quantity (IQ), logistic regression (LR), frequency ratio (FR), artificial neural network (ANN), random forest (RF), support vector machine (SVM), and convolutional neural network (CNN) methods were performed to construct the LSM. The results showed that the distance to a river, slope angle, distance from structure, and engineering geological rock group were the main factors controlling landslide development. A comprehensive set of statistical indicators was employed to evaluate these methods’ effectiveness; sensitivity, F1-measure, and AUC (area under the curve) were calculated and subsequently compared to assess the performance of the methods. Machine learning methods’ training and prediction accuracy were higher than those of statistical methods. The AUC values of the IQ, FR, LR, BP-ANN, RBF-ANN, RF, SVM, and CNN methods were 0.810, 0.854, 0.828, 0.895, 0.916, 0.932, 0.948, and 0.957, respectively. Although the performance order varied for other statistical indicators, overall, the CNN method was the best, while the BP-ANN and RBF-ANN method was the worst among the five examined machine methods. Hence, adopting the CNN approach in this study can enhance LSM accuracy, catering to the needs of planners and government agencies responsible for managing landslide-prone areas and preventing landslide-induced disasters.

The structure of a material is an important factor in determining its physical properties. Here, we adjust the structure of the Ni50Mn37Ga13 spun ribbons by changing the wheel speed to regulate the exchange bias effect of the material. The characterization results of micromorphology and structure show that as the wheel speed increases, the martensite lath decreases from 200 nm to 50 nm, the structure changed from the NM to a NM and 10M mixed martensitic structure containing mainly NM, then changed to NM and 10M where 10M and NM are approaching. Meanwhile, HE first increased and then decreased as the wheel speed increased. The optimum exchange bias effect (HE = 7.2 kOe) occurs when the wheel speed is 25 m∙s−1, mainly attributed to the enhanced ferromagnetism caused by part of 10M in NM martensite, which enhanced the exchange coupling of ferromagnetism and antiferromagnetism. This work reveals the structural dependence of exchange bias and provides a way to tune the magnitude of the exchange bias of Heusler alloys.

Medical image segmentation plays a crucial role in clinical diagnosis, treatment planning, and disease monitoring. The automatic segmentation method based on deep learning has developed rapidly, with segmentation results comparable to clinical experts for large objects, but the segmentation accuracy for small objects is still unsatisfactory. Current segmentation methods based on deep learning find it difficult to extract multiple scale features of medical images, leading to an insufficient detection capability for smaller objects. In this paper, we propose a context feature fusion and attention mechanism based network for small target segmentation in medical images called CFANet. CFANet is based on U-Net structure, including the encoder and the decoder, and incorporates two key modules, context feature fusion (CFF) and effective channel spatial attention (ECSA), in order to improve segmentation performance. The CFF module utilizes contextual information from different scales to enhance the representation of small targets. By fusing multi-scale features, the network captures local and global contextual cues, which are critical for accurate segmentation. The ECSA module further enhances the network’s ability to capture long-range dependencies by incorporating attention mechanisms at the spatial and channel levels, which allows the network to focus on information-rich regions while suppressing irrelevant or noisy features. Extensive experiments are conducted on four challenging medical image datasets, namely ADAM, LUNA16, Thoracic OAR, and WORD. Experimental results show that CFANet outperforms state-of-the-art methods in terms of segmentation accuracy and robustness. The proposed method achieves excellent performance in segmenting small targets in medical images, demonstrating its potential in various clinical applications.

High-temperature stress is the main environmental stress that restricts the growth and development of woody plants, and the growth and development of woody plants are affected by high-temperature stress. The influence of high temperature on woody plants varies with the degree and duration of the high temperature and the species of woody plants. Woody plants have the mechanism of adapting to high temperature, and the mechanism for activating tolerance in woody plants mainly counteracts the biochemical and physiological changes induced by stress by regulating osmotic adjustment substances, antioxidant enzyme activities and transcription control factors. Under high-temperature stress, woody plants ability to perceive high-temperature stimuli and initiate the appropriate physiological, biochemical and genomic changes is the key to determining the survival of woody plants. The gene expression induced by high-temperature stress also greatly improves tolerance. Changes in the morphological structure, physiology, biochemistry and genomics of woody plants are usually used as indicators of high-temperature tolerance. In this paper, the effects of high-temperature stress on seed germination, plant morphology and anatomical structure characteristics, physiological and biochemical indicators, genomics and other aspects of woody plants are reviewed, which provides a reference for the study of the heat-tolerance mechanism of woody plants.

Safety and reliability are vital for robotic fish, which can be improved through fault diagnosis. In this study, a method for diagnosing sensor faults is proposed, which involves using Gramian angular field fusion with particle swarm optimization and lightweight AlexNet. Initially, one-dimensional time series sensor signals are converted into two-dimensional images using the Gramian angular field method with sliding window augmentation. Next, weighted fusion methods are employed to combine Gramian angular summation field images and Gramian angular difference field images, allowing for the full utilization of image information. Subsequently, a lightweight AlexNet is developed to extract features and classify fused images for fault diagnosis with fewer parameters and a shorter running time. To improve diagnosis accuracy, the particle swarm optimization algorithm is used to optimize the weighted fusion coefficient. The results indicate that the proposed method achieves a fault diagnosis accuracy of 99.72% when the weighted fusion coefficient is 0.276. These findings demonstrate the effectiveness of the proposed method for diagnosing depth sensor faults in robotic fish.

The efficient utilization of solar energy significantly contributes to energy efficiency in buildings. Solar photovoltaic thermal (PVT) heat pumps, a hybrid of photovoltaic and solar-assisted heat pumps, have demonstrated a significant development trend due to their multi-generational capacity for heating, power, and cooling with reliable operational performance. This research work presents and investigates a single-stage compression PVT heat pump system, along with the operation principle of the system’s heating and power co-generation throughout the winter and transitional season. The construction of the testing facility, data reduction, error analysis, and performance evaluation indices of the system are all explained theoretically. A continuous experiment research project focusing on system heating and power performance was carried out in Dalian during the transition season (November in this study) and winter season (December in this study) as part of our investigation into the potential uses for space heating, residential hot water, and power supply in northern China. The findings of the experimental research demonstrate that the proposed system can generate electricity and heat at high efficiency during the winter and transitional seasons, with long-term stable performance. The system’s average heating COPt is 5 during the transitional season and 4.4 during the winter season. Meanwhile, the average photovoltaic power efficiency under both weather conditions is 11.9% and 10.2%, with a peak value of 15.7% and 12.0%, respectively. Additionally, the system compression ratio’s variation range is 2 to 3.88, which is lower than the standard heat pump system. As a result, the entire system heating operating process remains constant.

Monitoring and analyzing radio interference sources play a crucial role in ensuring the safe operation of civil aviation navigation, communication, airport management, and air traffic control. Traditional ground monitoring methods are slow and inadequate for tracking aerial and mobile interference sources effectively. Although flight methods such as helicopters and airships can effectively monitor aerial interference, the flight approval process is time-consuming and expensive. This paper investigates a novel approach to locating civil aviation radio interference sources using four unmanned aerial vehicles (UAVs) to address this issue. It establishes a model for aerial positioning of radio interference sources with the four UAVs and proposes a method for time synchronization and data communication among them. The paper conducts simulations of the four-UAV time–frequency difference positioning method, analyzing the geometric accuracy dilution with different deployment configurations of the UAVs, positioning biases, and root mean square errors (RMSEs) under varying interference source movement speeds. The simulation results provide crucial data to support subsequent experiments.

MicroRNA-491-5p (miR-491-5p) plays an important role in regulating cell proliferation and migration; however, the effect of miR-491-5p on neovascularization after traumatic brain injury remains poorly understood. In this study, a controlled cortical injury model in C57BL/6 mice and an oxygen-glucose deprivation model in microvascular endothelial cells derived from mouse brain were established to simulate traumatic brain injury in vivo and in vitro, respectively. In the in vivo model, quantitative real-time-polymerase chain reaction results showed that the expression of miR-491-5p increased or decreased following the intracerebroventricular injection of an miR-491-5p agomir or antagomir, respectively, and the expression of miR-491-5p decreased slightly after traumatic brain injury. To detect the neuroprotective effects of miR-491-p, neurological severity scores, Morris water maze test, laser speckle techniques, and immunofluorescence staining were assessed, and the results revealed that miR-491-5p downregulation alleviated neurological dysfunction, promoted the recovery of regional cerebral blood flow, increased the number of lectin-stained microvessels, and increased the survival of neurons after traumatic brain injury. During the in vitro experiments, the potential mechanism of miR-491-5p on neovascularization was explored through quantitative real-time-polymerase chain reaction, which showed that miR-491-5p expression increased or decreased in brain microvascular endothelial cells after transfection with an miR-491-5p mimic or inhibitor, respectively. Dual-luciferase reporter and western blot assays verified that metallothionein-2 was a target gene for miR-491-5p. Cell counting kit 8 (CCK-8) assay, flow cytometry, and 2?,7?-dichlorofluorescein diacetate (DCFH-DA) assay results confirmed that the downregulation of miR-491-5p increased brain microvascular endothelial cell viability, reduced cell apoptosis, and alleviated oxidative stress under oxygen-glucose deprivation conditions. Cell scratch assay, Transwell assay, tube formation assay, and western blot assay results demonstrated that miR-491-5p downregulation promoted the migration, proliferation, and tube formation of brain microvascular endothelial cells through a metallothionein-2-dependent hypoxia-inducible factor-1α/vascular endothelial growth factor pathway. These findings confirmed that miR-491-5p downregulation promotes neovascularization, restores cerebral blood flow, and improves the recovery of neurological function after traumatic brain injury. The mechanism may be mediated through a metallothionein-2-dependent hypoxia-inducible factor-1α/vascular endothelial growth factor signaling pathway and the alleviation of oxidative stress. All procedures were approved by Ethics Committee of the First Affiliated Hospital of Chongqing Medical University, China (approval No. 2020-304) on June 22, 2020.

In early brain injury (EBI) after subarachnoid hemorrhage (SAH), white matter (WM) axonal injury plays a key role in the prognosis of the disease. The purpose of this study was to investigate the effects of phosphatase and tensin homolog deleted on chromosome ten (PTEN) on axonal injury and neuronal apoptosis post-SAH in rats and to find its underlying mechanism. Adeno-associated virus was injected into the lateral ventricle to suppress or promote PTEN. Neural function post-SAH in animals was determined by the modified Garcia score, beam balance, and Rotarod test, and the blood–brain barrier disruption was assessed by the brain water content. Axonal injury post-SAH was observed by TEM and determined by IF, and neuron apoptosis was measured by TUNEL staining. The mechanism was analyzed by Western blot to detect p-PTEN/PTEN, p-AKT/AKT, p-GSK-3β/GSK-3β, p-CRMP-2/CRMP-2, axonal injury marker β-APP and pro- and anti-apoptosis proteins, including Bax and Bcl-2, expression. We found 1. After knocking down PTEN, neuronal apoptosis and axonal injury were alleviated, and nerve function and blood–brain barrier were protected; accordingly, after overexpression of PTEN, neuronal apoptosis and axon damage were aggravated, and nerve function damage and blood–brain barrier damage were increased. 2. PTEN and AKT/GSK-3β/CRMP-2 pathway were jointly involved in regulating neuronal apoptosis and WM axon injury after SAH. According to our research, PTEN was a negative factor of EBI, and together with the AKT/GSK-3β/CRMP-2 signaling pathway aggravates neuronal apoptosis and WM axon damage after SAH. Inhibition of PTEN expression may become a new target for SAH treatment.

Small towns are the bridge between urban and rural areas, and their sewage treatment is the focus and problem of urban construction. With Chongqing as the research object, this study explores the influencing factors and the characteristics of temporal and spatial changes in sewage treatment rates of 756 small towns in Chongqing from 2014 to 2020 from four aspects: the overall situation, space, population, and industry. The results show that 1) the sewage treatment rates of small towns showed a certain trend of improvement since 2014 and that the polarization between small towns continued to decrease. 2) The sewage treatment rates of small towns presented a significant spatial correlation in their entirety, but the correlation weakened year by year. In 2014, the sewage treatment rates of small towns were mainly high-high clusters, while those in 2017 were mainly low-low clusters and those in 2020 were low-low clusters in a scattered manner. 3) The sewage treatment rates of small towns with a population of 40,000–60,000 and over 60,000 in 2014 were relatively high, but the gap narrowed year by year. Small towns with more than 60,000 people had the highest sewage treatment rate in 2020, but the gap between the lowest values was reduced to 11.94%. 4) In 2014, small towns with tourism as the leading industry had a significant advantage, but three types of small towns, including industrial and mining towns, were improving fast. 5) Land urbanization, the radiation effect of county towns, the investment in urban construction, population size, and leading industries all had notable driving effects on the increase in sewage treatment rates of small towns, but the impact of urban construction investment was relatively weak because it contained a large scale of real estate investment.

The 14th Five-Year Plan stated that China should accelerate green development and promote a comprehensive green transition in economic and social development. As China's economic growth slows, green development becomes greatly significant for the high-quality development of the economy. Based on China's provincial panel data from 2005 to 2021, this study applies fixed effects model and mediating effect model to explore the influence of the government environmental investment on green development. The results indicate that (1) the government environmental investment was conducive to green development, but such effect weakened with time. (2) The government environmental investment indirectly promoted green development through the application of green patents and economic agglomeration. (3) The promotional effect of the government environmental investment varied according to region and time. Specifically, investment exerted the most significant effect on the green development of eastern China, which became more evident after 2015. The government should promote green development by implementing long-term assessment and accountability mechanisms, expanding the scale of economic agglomeration, improving the efficiency of the application of green patents, and improving the accuracy of environmental investment.

Semiconductor‐based photocatalytic water splitting is one of the effective ways for future hydrogen (H2) production. However, the wide bandgap of most semiconductors severely limits the solar spectral absorption range, which seriously hinders their practical applications. Herein, porous N‐doped K2Nb2O6 nanocrystals with defective pyrochlore structure are rationally designed to extend the absorption range from ultraviolet to visible region around 550 nm by NH3 heat treatment. The obtained 6N–K2Nb2O6 shows the highest visible‐light activity with an H2 evolution rate of 20.4 μmol h−1 g−1. More detailed exploration demonstrates that NH3 heat treatment can promote the substitutional nitrogen doping in K2Nb2O6 crystals while accompanied by the formation of oxygen vacancies or Nb4+ species. The hybridization of N 2p and O 2p orbitals in the valence band (VB) of the nitrogen‐doped K2Nb2O6 makes the VB maximum move more negatively, whereas the conduction band minimum shifted more positively due to the role of defective Nb4+ species. Such a synergistic effect endows the samples with visible‐light absorption and greatly enhances charge separation and transfer efficiency, resulting in the excellent H2 production performance. This study provides additional insights into the understanding of the effect of nitrogen doping on photocatalysts with excellent visible‐light‐driven photocatalytic activity.

Cardiomyopathy (CM) is a group of diseases distinguished by morphological and functional abnormalities in the myocardium. It is etiologically heterogeneous and may develop via cell autonomous and/or non-autonomous mechanisms. One of the most severe forms of CM has been linked to the deficiency of the ubiquitously expressed RNase Z endoribonuclease. RNase Z cleaves off the 3'-trailer of both nuclear and mitochondrial primary tRNA (pre-tRNA) transcripts. Cells mutant for RNase Z accumulate unprocessed pre-tRNA molecules. Patients carrying RNase Z variants with reduced enzymatic activity display a plethora of symptoms including muscular hypotonia, microcephaly and severe heart hypertrophy; still, they die primarily due to acute heart decompensation. Determining whether the underlying mechanism of heart malfunction is cell autonomous or not will provide an opportunity to develop novel strategies of more efficient treatments for these patients. In this study, we used CRISPR-TRiM technology to create Drosophila models that carry cardiomyopathy-linked alleles of RNase Z only in the cardiomyocytes. We found that this modification is sufficient for flies to develop heart hypertrophy and systolic dysfunction. These observations support the idea that the RNase Z linked CM is driven by cell autonomous mechanisms.

Mucopolysaccharidosis (MPS) is a progressive genetic disease that causes a deficiency in lysosomal enzymes, which play an important role in the degradation pathway of glycosaminoglycans. As a result of enzyme defects, mucopolysaccharides cannot be metabolized and thus accumulate. The cervical spine is one of the most commonly involved sites; thus, prompt surgical management before the onset of severe neurological deterioration is critical. However, because of the rarity of the disease, there is no standard treatment. In this review, we characterize the cervical spinal involvement in pediatric patients with MPS, describe the useful imaging technologies for diagnosis, and provide screening procedure for children with MPS. Surgical managements, including indications, surgical methods, possible difficulties, and solutions, are reviewed in detail.

Abstract Messenger RNA (mRNA) vaccine technology has shown its power in preventing the ongoing COVID-19 pandemic. Two mRNA vaccines targeting the full-length S protein of SARS-CoV-2 have been authorized for emergency use. Recently, we have developed a lipid nanoparticle-encapsulated mRNA (mRNA-LNP) encoding the receptor-binding domain (RBD) of SARS-CoV-2 (termed ARCoV), which confers complete protection in mouse model. Herein, we further characterized the protection efficacy of ARCoV in nonhuman primates and the long-term stability under normal refrigerator temperature. Intramuscular immunization of two doses of ARCoV elicited robust neutralizing antibodies as well as cellular response against SARS-CoV-2 in cynomolgus macaques. More importantly, ARCoV vaccination in macaques significantly protected animals from acute lung lesions caused by SARS-CoV-2, and viral replication in lungs and secretion in nasal swabs were completely cleared in all animals immunized with low or high doses of ARCoV. No evidence of antibody-dependent enhancement of infection was observed throughout the study. Finally, extensive stability assays showed that ARCoV can be stored at 2–8 °C for at least 6 months without decrease of immunogenicity. All these promising results strongly support the ongoing clinical trial.

Vaccination of children with special health status has become one of the most urgent issues in China. We aim to evaluate vaccination coverage and safety as well as its associated factors among children with special health status in China during 2016‒2020. We conducted a retrospective cohort review of all children with special health status recorded in the Electronic Immunization Registries System in Chongqing, China, between 2016 and 2020. Univariate and multivariate logistic regression analyses were used to analyze the influence factors. Among the 2,175 children with special health status enrolled in the study, the overall vaccination coverage rate was lower than that among the general population, and the incidence of adverse event in them following immunization was very rare. Children with congenital heart disease were better vaccinated (aOR = 1.508–6.331), while most of the jaundice children had missed vaccination (aOR = 0.441‒0.556). The purchase of vaccine compensation insurance was associated with higher completion rate of basic immunization for Bacillus Calmette-G vaccine (aOR = 1.706, 95% CI: 1.249‒2.329) and rotavirus vaccine (aOR = 1.346, 95% CI: 1.061‒1.708). Children with special health status can be safely vaccinated. However, the vaccination coverage in these huge and vulnerable group is too low to protect them from vaccine-preventable diseases through immunization. More researches and interventions should be conducted to ensure a higher vaccination rate among the children with special health status.