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Urban land‐use change has the potential to affect local to global biogeochemical carbon (C) and nitrogen (N) cycles and associated greenhouse gas (GHG) fluxes. We conducted a meta‐analysis to (1) assess the effects of urbanization‐induced land‐use conversion on soil nitrous oxide (N2O) and methane (CH4) fluxes, (2) quantify direct N2O emission factors (EFd) of fertilized urban soils used, for example, as lawns or forests, and (3) identify the key drivers leading to flux changes associated with urbanization. On average, urbanization increases soil N2O emissions by 153%, to 3.0 kg N ha−1 year−1, while rates of soil CH4 uptake are reduced by 50%, to 2.0 kg C ha−1 year−1. The global mean annual N2O EFd of fertilized lawns and urban forests is 1.4%, suggesting that urban soils can be regional hotspots of N2O emissions. On a global basis, conversion of land to urban greenspaces has increased soil N2O emission by 0.46 Tg N2O‐N year−1 and decreased soil CH4 uptake by 0.58 Tg CH4‐C year−1. Urbanization driven changes in soil N2O emission and CH4 uptake are associated with changes in soil properties (bulk density, pH, total N content, and C/N ratio), increased temperature, and management practices, especially fertilizer use. Overall, our meta‐analysis shows that urbanization increases soil N2O emissions and reduces the role of soils as a sink for atmospheric CH4. These effects can be mitigated by avoiding soil compaction, reducing fertilization of lawns, and by restoring native ecosystems in urban landscapes. [ABSTRACT FROM AUTHOR]

Anthropogenic activities increase the contributions of inland waters to global greenhouse gas (GHG; CO2, CH4, and N2O) budgets, yet the mechanisms driving these increases are still not well constrained. In this study, we quantified year-long GHG concentrations and fluxes, as well as water physico-chemical variables from 23 streams, 3 ditches, and 2 wastewater inflow sites across five headwater catchments in Germany contrasted by land use. Using mixed-effects models, we determined the overall impact of land use and seasonality on the intra-annual variabilities of these parameters. We found that land use was more significant than seasonality in controlling the intra-annual variability of GHG concentrations and fluxes. Agricultural land use and wastewater inflows in settlement areas resulted in up to 10 times higher daily riverine CO2, CH4, and N2O emissions than forested areas, as substrate inputs by these sources appeared to favor in situ GHG production processes. Dissolved GHG inputs directly from agricultural runoff and waste-water inputs also contributed substantially to the annual emissions from these sites. Drainage ditches were hotspots for CO2 and CH4 fluxes due to high dissolved organic matter concentrations, which appeared to favor in situ production via respiration and methanogensis. Overall, the annual emission from anthropogenic-influenced streams in CO2-equivalents was up to 20 times higher (~71 kg CO2 m-2 yr-1) than from natural streams (~3 kg CO2 m-2 yr-1). Future studies aiming to estimate the contribution of lotic ecosystems to GHG emissions should therefore focus on anthropogenically perturbed streams, as their GHG emission are much more variable in space and time. [ABSTRACT FROM AUTHOR]

The assessment of cropland carbon and nitrogen (C & N) balances play a key role to identify cost effective mitigation measures to combat climate change and reduce environmental pollution. In this paper, a biogeochemical modelling approach is adopted to assess all C & N fluxes in a regional cropland ecosystem of Thessaly, Greece. Additionally, the estimation and quantification of the modelling uncertainty in the regional inventory are realized through the propagation of parameter distributions through the model leading to result distributions for modelling estimations. The model was applied on a regional dataset of approximately 1000 polygons deploying model initializations and crop rotations for the 5 major crop cultivations and for a timespan of 8 years. The full statistical analysis on modelling results yields for the C balance carbon input fluxes into the soil of 12.4 ± 1.4 tons C ha-1 yr-1 and output fluxes of 11.9 ± 1.3 tons C ha-1 yr-1, with a resulting average carbon sequestration of 0.5 ± 0.3 tons C ha-1 yr-1. The averaged N influx was 212.3 ± 9.1 kg N ha-1 yr-1 while outfluxes were estimated on average of 198.3 ± 11.2 kg N ha-1 yr-1. The net N accumulation into the soil nitrogen pools was estimated to 14.0 ± 2.1 kg N ha-1 yr-1. The N outflux consist of gaseous N fluxes composed by N2O emissions 2.6 ± 0.8 kg N2O-N ha-1 yr-1, NO emissions of 3.2 ± 1.5 kg NO-N ha-1 yr-1, N2 emissions 15.5 ± 7.0 kg N2-N ha-1 yr-1 and NH3 emissions of 34.0 ± 6.7 kg NH3-N ha-1 yr-1, as well as aquatic N fluxes (only nitrate leaching into surface waters) of 14.± 4.5 kg NO3-N ha-1 yr-1, N fluxes of N removed from the fields in yields, straw and feed of 128.8 ± 8.5 kg N ha-1 yr-1. [ABSTRACT FROM AUTHOR]

Background: Arbuscular mycorrhizal fungi (AMF) are key soil organisms and their extensive hyphae create a unique hyphosphere associated with microbes actively involved in N cycling. However, the underlying mechanisms how AMF and hyphae-associated microbes may cooperate to influence N2O emissions from "hot spot" residue patches remain unclear. Here we explored the key microbes in the hyphosphere involved in N2O production and consumption using amplicon and shotgun metagenomic sequencing. Chemotaxis, growth and N2O emissions of isolated N2O-reducing bacteria in response to hyphal exudates were tested using in vitro cultures and inoculation experiments. Results: AMF hyphae reduced denitrification-derived N2O emission (max. 63%) in C- and N-rich residue patches. AMF consistently enhanced the abundance and expression of clade I nosZ gene, and inconsistently increased that of nirS and nirK genes. The reduction of N2O emissions in the hyphosphere was linked to N2O-reducing Pseudomonas specifically enriched by AMF, concurring with the increase in the relative abundance of the key genes involved in bacterial citrate cycle. Phenotypic characterization of the isolated complete denitrifying P. fluorescens strain JL1 (possessing clade I nosZ) indicated that the decline of net N2O emission was a result of upregulated nosZ expression in P. fluorescens following hyphal exudation (e.g. carboxylates). These findings were further validated by re-inoculating sterilized residue patches with P. fluorescens and by an 11-year-long field experiment showing significant positive correlation between hyphal length density with the abundance of clade I nosZ gene. Conclusions: The cooperation between AMF and the N2O-reducing Pseudomonas residing on hyphae significantly reduce N2O emissions in the microsites. Carboxylates exuded by hyphae act as attractants in recruiting P. fluorescens and also as stimulants triggering nosZ gene expression. Our discovery indicates that reinforcing synergies between AMF and hyphosphere microbiome may provide unexplored opportunities to stimulate N2O consumption in nutrient-enriched microsites, and consequently reduce N2O emissions from soils. This knowledge opens novel avenues to exploit cross-kingdom microbial interactions for sustainable agriculture and for climate change mitigation. C1gn2zbPQXat79TnoeAsNQ Video Abstract [ABSTRACT FROM AUTHOR]