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Objective To evaluate the role of ultrasound in prenatal diagnosis of vasa praevia (VP) and to asses the risk of VP associated with different causal factors. Material and Methods A retrospective study of the incidence of VP in a series of 12 063 deliveries between January 2000 and March 2005. We also studied the factors that predisposed for VP and the perinatal outcome of pregnancies. Results The prevalence of VP in our centre during this period was 0.07% (9 cases). All cases were prenatally diagnosed. The mean gestational age at diagnosis was 26 weeks. Multivariate analysis revealed the following associated factors: IVF pregnancies, bilobate or succenturiate placenta, and second-trimester placenta praevia, with an odds ratio of 7.75, 22.11 and 22.86, respectively. Conclusions In our series, the prenatal diagnosis of all cases of VP achieved during the second-trimester scan allowed us to avoid any prenatal death related to this condition. Copyright © 2007 John Wiley & Sons, Ltd.

1042 Background: Neoadjuvant chemotherapy is generally used for treatment of both early stage and locally advanced breast cancer. However, robust prognostic or predictive biomarkers are needed in t...

P0307 MELATONIN-INDUCED APOPTOSIS OF HepG2 CELLS IS ENHANCED BY AUTOPHAGY SUPPRESSION R. Ordonez, A. Fernandez, L. Martinez, S. Nunez, S. CarbajoPescador, A. Baulies, N. Prieto-Dominguez, C. Garcia-Ruiz, J.C. Fernandez-Checa, J.L. Mauriz, J. Gonzalez-Gallego. Institute of Biomedicine (IBIOMED), University of Leon (Spain), Leon, Centro de Investigacion Biomedica en Red: Enfermedades hepaticas y Digestivas (CIBERehd). Instituto de Salud Carlos III, Madrid, Department of Cell Death and Proliferation, Instituto Investigaciones Biomedicas de Barcelona, CSIC, Barcelona, and Liver Unit-Hospital Clinic-IDIBAPS, Barcelona, Spain E-mail: rordf@unileon.es

OBJECTIVE: To assess the response rate to a multidimensional, self administered questionnaire in patients ≥75 years old attending a primary health care center and to establish the prevalences of problems in the following dimensions: socioeconomical, cognitive, morbidity, polypharmacy, physical activity, falls, and activities of the daily living. DESIGN: Cross-sectional descriptive study based on a self-administered questionnaire posted by mail. SETTING: A primary health care center in Barcelona. Spain. PARTICIPANTS: A total of 1299 patients ≥75 years old. Main measurements Social surroundings, activities of the daily living, sensorial problems, morbidity, physical symptoms including sphincter's incontinence, cognitive status, and medication use. RESULTS: The response rate was 68% (95% CI, 0.65-0.70). The average age of the women who participated was significantly greater than the one of the men (82.5±5.3 vs 81.6±5.0, respectively). A significantly greater proportion of women than of men lived single (38.2 vs 10.9%); they had difficulties to maintain its house cosy (15.6 vs 8.8%) and they had difficulties to make ends meet (18.0 vs 13.6%). Globally, there were a greater proportion of women than of men whom they had: medical problems, cognitive problems, depression, and difficulties to carry out activities of the daily living. Overall, subjects that have had difficulties to make ends meet had greater prevalences of problems in all the dimensions of this evaluation. On the other hand, subjects that lived single had lower prevalences of problems in all the dimensions except they had a greater prevalence of depression. CONCLUSION: The multidimensional evaluation in elderly patients by the postal method is an efficient procedure that allows identifying many socioeconomic and health problems. In addition, it is feasible to identify to most fragile subjects and latter on to carry out preventive and curative interventions on them as well as to make their follow up.

Goals: features of Libyan breast cancer patients have not been fully investigated. The aim of this study is to evaluate the expression patterns of estrogen receptors, progesterone receptors, as well as nuclear morphometric features in these cancers, and to correlate them with clinico-pathologic features and prognosis. Methods: 62 female Libyan breast cancer patients diagnosed between 2000 and 2006 were retrospectively studied. Their clinical and pathologic data were collected and analysed. Immunohistochemical evaluation of ER and PR expression was done. Nuclear morphometry was carried out. Results: Of the 62 patients, 10 were of lobular type, 43 were invasive ductal and 9 were other carcinoma types. 47 of 62 had lymph node involvement. Positive hormonal receptor expression was more common among lymph node negative than among lymph node positive tumours. ER and PR positive patients appeared to have a better survival than ER and PR negative patients. The most significance difference in respect to survival was found between completely negative hormonal stain (J score 0) and positive staining (J score 1, 2 and 3). Larger nuclear size parameters were associated with lymph node involvement and high grade tumours (p

Altres ajuts: CICYT/AMB94-0881 Se tomaron imágenes multiespectrales de bosques de Quercus ilex L. de la comarca del Alt Empordà (noreste de la península Ibérica) mediante el sensor aerotransportado CASI (Compact Airborne Spectrographic Imager). Posteriormente, se ajustaron modelos lineales por regresión entre valores de cobertura arbórea, biomasa de madera y diámetro medio, provenientes de medidas de campo, y valores de reflectancia ofrecidos por trece canales del sensor y por dos indices de vegetación. En ninguno de los modelos se llegó a un nivel de ajuste estadísticamente significativa (p < 0.05). Después de hacer un repaso de los posibles factores causantes de estos resultados, parece necesario profundizar en temas como la corrección radiométrica, los efectos de la heterogeneidad estructural del bosque y el muestreo de campo. Multispectral images were taken from Quercus ilex L. forests located in the north-east of the Iberian Peninsula by means of the Compact Airborne Spectrographic Imager (CASI). Linear regression models were made between three field measured variables (tree coverage, wood biomass and tree mean diameter) and reflectance values given by 13 bands and two vegetation indices. None of the built models achieved statistical significance (p < 0.05). After checking for factors responsible for these low fittings, it seems necessary to deeply analyze issues such as the radiometric correction, effects of forest structural heterogeneity and field sampling.

Landsat Thematic Maper (TM) imagery was used to estimate crop area using the regression method. Two different kind of estimates have been made; one using a Landsat TM image of the same year as the ground sample and the other using an image taken the year before. The results show that if the rate of land cover change is not great the use of an image of the year previous to the ground survey is a way of reducing the costs of the images acquisition without a great loss in the accuracy of the area estimation of the main crops compared to the conventional regression method. © 1997 Taylor & Francis Ltd.

e22119 Background: Although it is know that pCR following neoadjuvant chemotherapy is more frequent in some subtypes of breast cancer such as Triple Negative (TN) or erb2 tumors, the predictive role of gene expression and mutation status is not well defined in this setting. Methods: We analyzed samples from 41 patients (p) prospectively treated with neoadjuvant chemotherapy (sequential AC followed by weekly TXL, or inverse sequence, plus trastuzumab for erb2 positive p). Pathologic response (PR) was classified according to Miller-Payne and RCB criteria. Radiologic evaluation was performed by ultrasonography, dynamic MR and PET-TAC after each chemotherapy sequence. We performed expression analysis of AXL, BRCA1, RAP80, BIM, EZH2, ROR1, FGFR1, PTPN12, YAP, GAS6, beta-TRCP, HIF1 alpha and ZNF217 by RT-PCR, and mutational status of p53 and PI3K genes in pretreatment biopsies. Statistical analysis was performed using Mann-Whitney U and Pearson’s chi-squared tests. Results: pCR was detected in 5 p (3TN, 2 erb2) of 25 p (9 luminal A, 5 luminal B, 6 erb2 and 5 TN) evaluated for PR at time of submitting this abstract. TN tumors had lower levels of RAP80 (p=.0013), PTPN12 (p=.003), beta TRCP (p=.001), ZNF217 (p=.014) and YAP (p=.097). Luminal B tumors had low levels of YAP and the highest levels of FGFR1 (p=.09) and ZNF217 (p=.014). Luminal A tumors had high levels of beta-TRCP (p=.003). We found no differences in BRCA1, AXL, BIM, EZH2, ROR1, GAS6 and HIF1 levels by breast cancer subtype. P with low levels of FGFR1 (p=.087), HIF1alpha (p=.07) or EZH2 (p=.005) had higher probability of pCR. No pCR was observed in p with higher levels of AXL, EZH2, RAP80, GAS6, beta TRCP, HIF alpha. Four p had p53 mutations (1 luminal B, 1 erb2 and 2 TN) and 4 p had PI3K mutations (2 luminal A, 1 erb2, 1 luminal B). There was no correlation between p53 status and PR. P with PI3K mutations did not achieve pCR vs 46% of p with wild type PI3K (p=.23). Conclusions: Gene expression profile varies by breast cancer subtype. Chemosensitivity could be higher in tumors with lower levels of FGFR1, HIF1alpha or EZH2. Further results will be presented.

Resumen del poster presentado a The International Liver Congress – 48th Annual meeting of the European Association for the Study of the Liver celebrado en Paises Bajos en abril de 2013.