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The posterior oblique ligament (POL) is the main component of the posteromedial corner (PMC) of the knee and plays a crucial role in acting as a secondary restraint against translation, rotation, and valgus forces. Injuries to the PMC often occur in association with acute or chronic deficiency of the anterior cruciate ligament and may result in anteromedial rotatory instability. A surgical technique for treatment of acute and chronic injuries of the posteromedial structures was first established by Hughston in 1973. This procedure involves an advancement and reefing of the POL and adherent posterior capsule to the stout tissue of the intact medial collateral ligament, potentially using suture anchors if the POL tissue is detached from bone. Additionally, in cases of residual laxity, the semimembranosus tendon may be advanced anteriorly to improve dynamic stabilization. This procedure appears to be useful in cases of moderate posteromedial insufficiency and avoids retrieval of a medial tendon graft from the region of the medially stabilizing hamstrings or from the healthy contralateral side.

The biomechanical consequences of a tear of the posterior root of the medial meniscus are comparable to that of a complete meniscectomy. The integrity of the meniscal roots is crucial to enable the important function of load sharing and shock absorption. An untreated root tear leads to extrusion and loss of function of the meniscus causing early degenerative arthritis of the respective knee compartment. Meniscal root repair can be achieved by 2 main techniques: indirect fixation using pullout sutures through a transtibial tunnel with extracortical fixation and direct fixation using suture anchors. Pullout sutures are prone to elongation or abrasion of the suture material due to the length of the bone tunnel. Current suture anchor techniques are challenging as they require an additional posterior portal with higher risk of damage to neurovascular structures. Even with the use of specially designed curved passing devices, secure insertion of the anchor is difficult. We present a technique for suture anchor refixation of the posterior root of the medial meniscus without the need for an additional posterior portal.

Background: Chemotherapy-induced peripheral neuropathy (CIPN) is a common and clinically relevant side effect of chemotherapy. Approximately 50% of all leukemia, lymphoma, colorectal- and breast cancer patients are affected. CIPN is induced by neurotoxic chemotherapeutic agents and can manifest with sensory and/or motor deficits. It is associated with significant disability and poor recovery. Common symptoms include pain, altered sensation, reduced or absent reflexes, muscle weakness, reduced balance control and insecure gait. These symptoms not only affect activities of daily living, subsequently reducing patients' quality of life, they have far more become a decisive limiting factor for medical therapy, causing treatment delays, dose reductions, or even discontinuation of therapy, which can affect the outcome and compromise survival. To date, CIPN cannot be prevented and its occurrence presents a diagnostic dilemma since approved and effective treatment options are lacking. Promising results have recently been achieved with exercise. We have revealed that sensorimotor training (SMT) or whole body vibration (WBV) can reduce the symptoms of CIPN and attenuate motor and sensory deficits. We furthermore detected a tendency that it may also have a preventive effect on the onset of CIPN., Methods: We are therefore conducting a prospective, multicentre, controlled clinical trial involving 236 oncological patients receiving either oxaliplatin (N = 118) or vinca-alkaloid (N = 118) who are randomized to one of two interventions (SMT or WBV) or a treatment as usual (TAU) group. Primary endpoint is the time to incidence of neurologically confirmed CIPN. Secondary endpoints are pain, maintenance of the functionality of sensory as well as motor nerve fibres as well as the level of physical activity. The baseline assessment is performed prior to the first cycle of chemotherapy. Subsequent follow-up assessments are conducted at 12 weeks, after completion of chemotherapy, and at a 3-month follow-up. Patients who develop CIPN receive an additional assessment at this time point, as it represents the primary endpoint., Discussion: We hypothesize that SMT and WBV prevent the onset or delay the progression of CIPN, decrease the likelihood of dose reductions or discontinuation of cancer treatment and improve patients' quality of life., Trial Registration: Deutsche Register Klinischer Studien ( DRKS00006088 , registered 07.05.2014).

Tropical mountains are usually characterized by a vertically-arranged sequence of ecological belts, which, in contrast to temperate habitats, have remained relatively stable in space across the Quaternary. Such long-lasting patterning of habitats makes them ideal to test the role of environmental pressure in driving ecological and evolutionary processes. Using Sumatran freshwater mayfly communities, we test whether elevation, rather than other spatial factors (i.e. volcanoes, watersheds) structures both species within communities and genes within species. Based on the analysis of 31 mayfly (Ephemeroptera) communities and restriction-site-associated-DNA sequencing in the four most ubiquitous species, we found elevation as the major spatial component structuring both species and genes in the landscape. In other words, similar elevations across different mountains or watersheds harbor more similar species and genes than different elevations within the same mountain or watershed. Tropical elevation gradients characterized by environmental conditions that are both steep and relatively stable seasonally and over geological time scales, are thus responsible for both ecological and genetic differentiation. Our results demonstrate how in situ ecological diversification at the micro-evolutionary level might fuel alpha- and beta- components of diversity in tropical sky islands.

Weevils (Curculionoidea) comprise one of the most diverse groups of organisms on earth. There is hardly a vascular plant or plant part without its own species of weevil feeding on it and weevil species diversity is greater than the number of fishes, birds, reptiles, amphibians and mammals combined. Here, we employ ultraconserved elements (UCEs) designed for beetles and a novel partitioning strategy of loci to help resolve phylogenetic relationships within the radiation of Australasian smurf-weevils (Eupholini). Despite being emblematic of the New Guinea fauna, no previous phylogenetic studies have been conducted on the Eupholini. In addition to a comprehensive collection of fresh specimens, we supplement our taxon sampling with museum specimens, and this study is the first target enrichment phylogenomic dataset incorporating beetle specimens from museum collections. We use both concatenated and species tree analyses to examine the relationships and taxonomy of this group. For species tree analyses we present a novel partitioning strategy to better model the molecular evolutionary process in UCEs. We found that the current taxonomy is problematic, largely grouping species on the basis of similar color patterns. Finally, our results show that most loci required multiple partitions for nucleotide rate substitution, suggesting that single partitions may not be the optimal partitioning strategy to accommodate rate heterogeneity for UCE loci.

Two new species and one new subspecies of Exocelina Broun, 1886 from New Guinea are described: E. okbapensis Shaverdo & Balke, sp. n. , E. okbapensis hajeki Shaverdo & Balke, ssp. n. , and E. may Shaverdo & Balke, sp. n. These and two already described species are assigned to the E. okbapensis -group, which is morphologically (based on setation of the paramere) and phylogenetically close to the E. aipo -group. On the latter, morphological and taxonomic notes are provided. An identification key to all known species of the groups is presented, and important diagnostic characters are illustrated. Data on the species distributions are mapped and show that the species occur only in the central mountain part of the island restricted by Wamena in the west and Sandaun Province in the east.

Mayflies, stoneflies and caddisflies (Ephemeroptera, Plecoptera and Trichoptera) are prominent representatives of aquatic macroinvertebrates, commonly used as indicator organisms for water quality and ecosystem assessments. However, unambiguous morphological identification of EPT species, especially their immature life stages, is a challenging, yet fundamental task. A comprehensive DNA barcode library based upon taxonomically well-curated specimens is needed to overcome the problematic identification. Once available, this library will support the implementation of fast, cost-efficient and reliable DNA-based identifications and assessments of ecological status. This study represents a major step towards a DNA barcode reference library as it covers for two-thirds of Germany's EPT species including 2,613 individuals belonging to 363 identified species. As such, it provides coverage for 38 of 44 families (86%) and practically all major bioindicator species. DNA barcode compliant sequences (≥500 bp) were recovered from 98.74% of the analysed specimens. Whereas most species (325, i.e., 89.53%) were unambiguously assigned to a single Barcode Index Number (BIN) by its COI sequence, 38 species (18 Ephemeroptera, nine Plecoptera and 11 Trichoptera) were assigned to a total of 89 BINs. Most of these additional BINs formed nearest neighbour clusters, reflecting the discrimination of geographical subclades of a currently recognized species. BIN sharing was uncommon, involving only two species pairs of Ephemeroptera. Interestingly, both maximum pairwise and nearest neighbour distances were substantially higher for Ephemeroptera compared to Plecoptera and Trichoptera, possibly indicating older speciation events, stronger positive selection or faster rate of molecular evolution., (© 2017 John Wiley & Sons Ltd.)

Background: Taxonomy and biogeography can benefit from citizen scientists. The use of social networking and open access cooperative publishing can easily connect naturalists even in more remote areas with in-country scientists and institutions, as well as those abroad. This enables taxonomic efforts without frontiers and at the same time adequate benefit sharing measures., New Information: We present new distribution and habitat data for diving beetles of Bali island, Indonesia, as a proof of concept. The species Hydaticus luczonicus Aubé, 1838 and Eretes griseus (Fabricius, 1781) are reported from Bali for the first time. The total number of Dytiscidae species known from Bali is now 34.

History  A 30-year-old man presented with a history of progressive muscle weakness, difficulty in concentrating, and a slender habitus since early childhood. Marfan syndrome was suspected since the age of 14. Examinations  13 years later he was examined by Marfan experts and by genetic testing and Marfan syndrome could not be confirmed. Further neurological examination revealed the suspected diagnosis of myotonic dystrophy type 1, which was confirmed by genetic testing. Treatment and course  Similar to Marfan syndrome, myotonic dystrophy is a multisystemic disorder with the risk of cardiac arrythmias. It is necessary to provide an interdisciplinary care by neurologists, internists, ophthalmologists, speech therapists, and physiotherapists. Conclusion  It is not enough to take the habitus as the principle sign to diagnose Marfan syndrome. Furthermore, it is essential to consider symptoms that are not typical for Marfan syndrome, such as cognitive deficiencies or progressive paresis., Competing Interests: Disclosure The authors report no conflicts of interest in this work., (© Georg Thieme Verlag KG Stuttgart · New York.)

The origin of the astonishing New Caledonian biota continues to fuel a heated debate among advocates of a Gondwanan relict scenario and defenders of late oceanic dispersal. Here, we study the origin of New Caledonian Trigonopterus flightless weevils using a multimarker molecular phylogeny. We infer two independent clades of species found in the archipelago. Our dating estimates suggest a Late Miocene origin of both clades long after the re-emergence of New Caledonia about 37 Ma. The estimation of ancestral ranges supports an ancestral origin of the genus in a combined region encompassing Australia and New Guinea with subsequent colonizations of New Caledonia out of New Guinea in the mid-Miocene. The two New Caledonian lineages have had very different evolutionary trajectories. Colonizers belonging to a clade of foliage dwellers greatly diversified, whereas species inhabiting leaf-litter have been less successful., Competing Interests: We declare we have no competing interests.

A reclassification of several species traditionally included in Rhantus Dejean, 1833 is given: Nartus Zaitzev, 1907 is re-instated as genus with one species in the Nearctic and one in the Palaearctic region; two new genera are erected: Meri-diorhantus n. gen., with M. antarcticus (Germain, 1854) n. comb., M. calidus (Fabricius, 1792) n. comb., M. limbatus (Aubé, 1838) n. comb., M. orbignyi (Balke, 1992) n. comb., M. validus (Sharp, 1882) n. comb. in the Neotropical region (M. calidus also in the southern USA and along the eastern coast as far north as New York City), and the monotypic Caperhantus n. gen., with C. cicurius (Fabricius, 1787) n. comb. in south Africa. Furthermore, nine Pacific and Australian Rhantus are transferred to the genus Carabdytes Balke, Hendrich & Wewalka, 1992: C. alutaceus (Fauvel, 1883) n. comb., C. guadalcanalensis (Balke, 1998) n. comb., C. monteithi (Balke, Wewalka, Alarie & Ribera, 2007) n. comb., C. novaecaledoniae (J. Balfour-Browne, 1944) n. comb., C. oceanicus (Balke, 1993b) n. comb., C. pacificus (Boisduval, 1835) n. comb., C. plantaris (Sharp, 1882) n. comb., C. poellerbauerae (Balke, Wewalka, Alarie & Ribera, 2007) n. comb. and C. pseudopacificus Balke, 1993b) n. comb. All changes are based on a previous comprehensive molecular phylogenetic analysis of Colymbetinae. Diagnostic characters are given for all genera mentioned above and each of them is illustrated with one or more habitus pictures. An updated key to all genera of Colymbetinae is also given.

Six new species of New Guinea Exocelina Broun, 1886 are described in this paper: E. iratoi sp. n. , E. likui sp. n. , E. pui sp. n. , E. pulukensis (Balke, 1998), sp. n. (Balke & Hendrich, 2001), and E. tomhansi Shaverdo, Panjaitan & Balke, 2016, they are found to form a monophyletic clade and be closely related to representatives of the sp. n. , and E. wigodukensis sp. n . Although different morphologically, together with Exocelina ascendens (Balke, 1998), E. bagus (Balke & Hendrich, 2001), and E. ransikiensis Shaverdo, Panjaitan & Balke, 2016, they are found to form a monophyletic clade and be closely related to representatives of the E. ekari -group, based on preliminary analysis of sequence data. An identification key to the species is provided, and important diagnostic characters are illustrated. The present data on the species' distribution show that most of them are local endemics.

Rompindessus jenisi Balke, Bergsten & Hendrich, gen. n. et sp. n. is described from near Rompin village in West Malaysia. The new genus is characterized by the presence of an occipital line and basal pronotal striae, the presence of a thick anterior bead on clypeus and two-segmented parameres as well as by the absence of basal elytral striae, the absence of sutural line on elytron, the absence of basal epipleural transverse carina, and the absence of longitudinal elytral carina. Moreover, male pro- and mesotarsus appear stout, and distinctly dilated laterally; the pronotum is comparably long and parallel-sided and the colour of beetle conspicuous dark orange. Leiodytes kualalipis Balke, Wang, Bergsten & Hendrich, sp. n. is described from West Malaysia (Pahang) and South Vietnam (Cat Tien). It is well characterized by its large size, elongate body and the form of the median lobe. Limbodessus fijiensis (J. Balfour-Browne, 1944), comb. n. described from Fiji is a new synonym of Limbodessus curviplicatus (Zimmermann, 1927) described from Samoa.

Introduction: The aim of this study was to evaluate the current state of treatment in traumatic anterior shoulder dislocation in Germany and to detect changes over the last 12 years., Methods: Seven hundred ninety-six trauma and/or orthopaedic departments were found in the German hospital directory 2012. The websites of each department were searched for the email address of the responsible shoulder surgeon (if applicable) or the head of the department. Seven hundred forty-six email addresses were found, and emails with the request to participate in an online survey were sent in January 2013. Six hundred seventy-five emails probably reached the correct addressee. Seventy-one emails were rejected, and no contact could be made. One-hundred ninety-one (28 %) participated in the study. The data were compared to similar data from a survey on shoulder dislocation performed in the same department and published in 2001., Results: After the first-time traumatic shoulder dislocation in patients aged younger than 30 years participating in sports, 14 % of the participants would prefer conservative treatment, 83 % arthroscopic, and 3 % open surgery. When surgery was indicated, arthroscopic Bankart repair was the treatment of choice for 93 % of the participants. In 2001, 27 % had indicated conservative treatment after the first-time shoulder dislocation in active patients younger than 30 years. When surgery was indicated, 66 % had performed arthroscopic and 34 % open stabilization. For the standard arthroscopic Bankart repair without concomitant injuries, 41 % of the participants use two and 54 % three suture anchors. Knotless anchors were preferred by 72 %. In the case of glenoid bone loss greater than 25 %, only 46 % perform a procedure for glenoid bone augmentation. Fifteen percent of the participants always recommended immobilization in external rotation after traumatic first-time shoulder dislocation., Conclusions: The majority of participants recommend arthroscopic Bankart repair with two or three suture anchors in young persons with the first-time dislocations. Compared to 2001 less recommend conservative treatment, complex "open" surgical procedures are no longer used. The knowledge that a Bankart procedure likely fails in significant glenoid bone loss is not implemented in the clinical practice. Thus, there is a need to educate surgeons on this topic.

Tiporus queenslandicus sp. n. is described from NE Queensland. The new species is similar to T. undecimmaculatus (Clark, 1862) and T. giuliani (Watts, 1978) from the Northern Territory and north-western Australia but well characterized by its larger size, more elongate habitus, and form of median lobe and parameres. Tiporus queenslandicus sp. n. is a lotic species being collected from rest pools of intermittent creeks and rivers with a sandy bottom. Important species-defining characters (habitus, median lobe, paramere, protibia of male) are illustrated. Together with T. queenslandicus sp. n. the genus comprises now 13 species.

Ten new species of Exocelina Broun, 1886 from New Guinea are described: Exocelina andakombensis sp. n. , Exocelina garaina sp. n. , Exocelina injiensis sp. n. , Exocelina kabwumensis sp. n. , Exocelina marawaga sp. n. , Exocelina posmani sp. n. , Exocelina tekadu sp. n. (Balke, 1998), which is now placed into the Exocelina varirata sp. n. (Balke, 2001) Exocelina wareaga sp. n. (Balke, 1998) Exocelina woitapensis sp. n (Balke, 1998) Exocelina danae -group (with Exocelina miriae (Balke, 1998) Exocelina rivulus -group are considered to belong to a single species, Exocelina damantiensis (Balke, 1998). Redescription of Exocelina danae -group, and three new synonyms are therefore proposed: Exocelina madangensis (Balke, 2001) syn. n ., Exocelina patepensis (Balke, 1998) syn. n ., and Exocelina rivulus (Balke, 1998) syn. n . Exocelina tarmluensis (Balke, 1998) syn. n . is a junior synonym of Exocelina danae (Balke, 1998). Redescription of Exocelina atratus (Balfour-Browne, 1939) is provided based on its type material. An identification key to all known species of the group is provided, and important diagnostic characters are illustrated. Data on the species distribution are given, showing that whilst most species are local endemics, Exocelina damantiensis is extremely widely distributed.

Purpose: Distinct characteristics of acromial morphology seem to be one factor for the development of degenerative supraspinatus tendon tears. Thus, it is questionable whether patients with traumatic tendon tears also present these parameters. The hypothesis of the present study was that the acromial morphology of patients with degenerative supraspinatus tendon tears differs from patients with traumatic tears., Methods: One hundred and thirty-six patients that were treated by arthroscopic rotator cuff repair from 2010 to 2013 were included in this study. Seventy-two patients had degenerative (group 1), and 64 had traumatic (group 2) supraspinatus tendon tears. On preoperative radiographs the Bigliani type, acromial slope, acromiohumeral (AH) distance, lateral acromial angle (LAA), acromion index (AI), and critical shoulder angle (CSA) were measured. Medians of these parameters as well as of age of both groups were compared using the t test., Results: The percentaged distribution of the Bigliani type differed (group 1 vs. 2: type 1: 18/38, type 2: 56/55, type 3: 26/8). All parameters showed significant differences between degenerative and traumatic tears. Slope: 21.2° (SD 7.6°) versus 19.2° (SD 7.9°, p = 0.026), AH distance: 8.4 mm (SD 2.3 mm) versus 9.9 mm (SD 1.9 mm, p = 0.0006), LAA: 77.0° (SD 4.0°) versus 82.5° (SD 4.7°, p < 0.0001), AI: 0.77 (SD 0.07) versus 0.73 (0.06, p = 0.0239), and CSA: 36.8° (SD 3.6°) versus 35.3° (SD 2.9°, p = 0.007). An LAA <70° or an AH distance of <5 mm only occurred in degenerative tears. Patients with degenerative tears were significantly older (60 vs. 54 years)., Conclusions: The hypothesis that the acromial morphology of patients with degenerative supraspinatus tendon tears differs from patients with traumatic tears was confirmed. Shoulders with degenerative tears show a narrower subacromial space and a larger lateral extension as well as a steeper angulation of the acromion than with traumatic tears. Thus, the results of this study support the theory of external impingement as a cause for degenerative rotator cuff tears., Level of Evidence: IV.

Purpose: The purpose of the present study was to determine the value of shoulder magnetic resonance imaging (MRI) obtained in the community setting interpreted by musculoskeletal radiologists in patients with shoulder pain initiated by a single non-dislocating shoulder trauma., Methods: In 56 of 61 consecutive patients who underwent shoulder arthroscopy due to pain after a single non-dislocating shoulder trauma, the data sets of non-contrast MRI were complete. These were retrospectively interpreted by three radiologists specialized on musculoskeletal MRI who were blinded for patients' history and who did not have access to the reports of arthroscopy. Standard evaluation forms were used to assess the MRIs for superior labrum anterior and posterior (SLAP) lesions, anterior or posterior labrum lesions, lesions of the long head of biceps tendon (LHB) and for partial tears of the supraspinatus tendon and the upper quarter of the subscapularis tendon. Quality of the MRI was assessed by each radiologist on a four-point scale., Results: The pooled sensitivity for the three radiologists for the detection of SLAP lesions was 45.0 %, for anterior or posterior labrum tears 77.8 and 66.7 %, for lesions of the LHB 63.2 % and for partial tears of the supraspinatus or subscapularis tendon tears 84.8 and 33.3 %. Corresponding inter-rater reliabilities were poor (SLAP lesions) to substantial (anterior labrum tears). Quality of MRI only influenced the accuracy for the detection of posterior labrum tears., Conclusion: A non-contrast shoulder MRI obtained in the community setting after non-dislocating shoulder trauma has a moderate sensitivity for most intraarticular pathologies when interpreted by musculoskeletal radiologists. Accuracy is dependent on the observer and not on the assessed quality., Level of Evidence: Case series, Level IV.

The underlying mechanisms responsible for the general increase in species richness from temperate regions to the tropics remain equivocal. Many hypotheses have been proposed to explain this astonishing pattern but additional empirical studies are needed to shed light on the drivers at work. Here we reconstruct the evolutionary history of the cosmopolitan diving beetle subfamily Colymbetinae, the majority of which are found in the Northern hemisphere, hence exhibiting an inversed latitudinal diversity gradient. We reconstructed a dated phylogeny using 12 genes, to investigate the biogeographical history and diversification dynamics in the Colymbetinae. We aimed to identify the role that phylogenetic niche conservatism plays in the inversed diversification pattern seen in this group. Our results suggest that Colymbetinae originated in temperate climates, which supports the hypothesis that their distribution is the result of an ancestral adaptation to temperate environmental conditions rather than tropical origins, and that temperate niche conservatism can generate and/or maintain inverse latitudinal diversity gradients.

The German Barcoding initiatives BFB and GBOL have generated a reference library of more than 16,000 metazoan species, which is now ready for applications concerning next generation molecular biodiversity assessments. To streamline the barcoding process, we have developed a meta-barcoding pipeline: We pre-sorted a single malaise trap sample (obtained during one week in August 2014, southern Germany) into 12 arthropod orders and extracted DNA from pooled individuals of each order separately, in order to facilitate DNA extraction and avoid time consuming single specimen selection. Aliquots of each ordinal-level DNA extract were combined to roughly simulate a DNA extract from a non-sorted malaise sample. Each DNA extract was amplified using four primer sets targeting the CO1-5' fragment. The resulting PCR products (150-400bp) were sequenced separately on an Illumina Mi-SEQ platform, resulting in 1.5 million sequences and 5,500 clusters (coverage ≥10; CD-HIT-EST, 98%). Using a total of 120,000 DNA barcodes of identified, Central European Hymenoptera, Coleoptera, Diptera, and Lepidoptera downloaded from BOLD we established a reference sequence database for a local CUSTOM BLAST. This allowed us to identify 529 Barcode Index Numbers (BINs) from our sequence clusters derived from pooled Malaise trap samples. We introduce a scoring matrix based on the sequence match percentages of each amplicon in order to gain plausibility for each detected BIN, leading to 390 high score BINs in the sorted samples; whereas 268 of these high score BINs (69%) could be identified in the combined sample. The results indicate that a time consuming presorting process will yield approximately 30% more high score BINs compared to the non-sorted sample in our case. These promising results indicate that a fast, efficient and reliable analysis of next generation data from malaise trap samples can be achieved using this pipeline.

Two new species of Exocelina Broun, 1886 from Papua New Guinea are described herein: Exocelina mondmillensis sp. n. and Exocelina pseudomarinae sp. n. They are placed into the Exocelina broschii-group based on the shovel/fork-like ventral sclerites of their median lobe. While the former has rather distinct combination of the morphological characters (inconspicuous dorsal punctation, thin apex of the median lobe and ventral sclerite of the median lobe with two tips of different length), the latter is very similar to already described species Exocelina marinae (Shaverdo, Sagata & Balke, 2005). All described species of the group are revised and a key to their identification is provided. Important diagnostic characters (habitus, color, protarsomeres 4-5, median lobes, and parameres) are illustrated. Data on the distribution of all species of the group are given showing that its representatives occur only in Papua New Guinea and most of them are widely distributed in it central part.

Exocelina manokwariensis sp. n. from West Papua is placed into the Exocelina ekari-group based on the structure of its male genitalia. The new species is described, including its three subspecies, from the mainland of West Papua, Waigeo Island, Batanta and Salawati Islands, and Bomberai peninsula. An identification key to the subspecies as well as data on species distribution are provided.

The Sunda Arc forms an almost continuous chain of islands and thus a potential dispersal corridor between mainland Southeast Asia and Melanesia. However, the Sunda Islands have rather different geological histories, which might have had an important impact on actual dispersal routes and community assembly. Here, we reveal the biogeographical history of hyperdiverse and flightless Trigonopterus weevils. Different approaches to ancestral area reconstruction suggest a complex east to west range expansion. Out of New Guinea, Trigonopterus repeatedly reached the Moluccas and Sulawesi transgressing Lydekker's Line. Sulawesi repeatedly acted as colonization hub for different segments of the Sunda Arc. West Java, East Java and Bali are recognized as distinct biogeographic areas. The timing and diversification of species largely coincides with the geological chronology of island emergence. Colonization was not inhibited by traditional biogeographical boundaries such as Wallace's Line. Rather, colonization patterns support distance dependent dispersal and island age limiting dispersal.

Background: A reduced coracohumeral distance (CHD) is thought to be responsible for subcoracoid impingement. This only accounts for degenerative tendon tears. In traumatic tears, the subcoracoid space should be normal., Hypothesis: The CHD in patients with traumatic subscapularis tendon tears is larger than that in patients with degenerative tears and does not differ from patients with an intact subscapularis tendon., Study Design: Cohort study; Level of evidence, 3., Methods: A total of 83 patients with arthroscopically certified subscapularis tendon tears were included in the study. Forty-four patients had degenerative causes (group 1), and 39 had traumatic causes (group 2). The control group consisted of 20 patients with traumatic supraspinatus tendon tears and arthroscopically proven, intact subscapularis tendons (group 3). On preoperative axial magnetic resonance imaging, the distance between the CHD was measured, and the values of the 3 groups were compared using the t test., Results: The mean (±SD) CHD in patients with degenerative subscapularis tendon tears was 8.6 ± 2.0 mm (range, 4.0-13.2 mm) and was significantly (P = .0003) smaller than that in patients with traumatic tears (10.2 ± 2.0 mm; range, 6.6-16.2 mm) or controls (10.4 ± 1.8 mm; range, 6.8-14.0 mm). The CHD of controls and patients with traumatic tears did not differ significantly (P = .7875). A CHD of less than 6 mm only occurred in patients with degenerative subscapularis tendon tears., Conclusion: The hypothesis that the CHD in patients with degenerative subscapularis tendon tears is significantly smaller than that in patients with traumatic tears or intact subscapularis tendons was confirmed. The CHD in patients with traumatic tears does not differ from that in controls. A CHD of less than 6 mm only occurs in patients with degenerative subscapularis tendon tears., (© 2015 The Author(s).)

Background: To date only one species of Limbodessus diving beetles has been reported from the Island of New Guinea, L. compactus (Clark, 1862), which is widerspread in the Australian region., New Information: We describe two new species of microendemic New Guinea Limbodessus and use a compact descriptive format flanked by enriched online content in wiki powered species pages. Limbodessus baliem sp.n. is described from ca. 1,600 m altitude in the Baliem Valley of Papua and Limbodessus alexanderi sp.n. from >3,000 m altitude north of Sugapa, Papua. Based on our analysis, we also transfer three species from other genera to Limbodessus Guignot, 1939, with the following changes: Limbodessus deflectus (Ordish, 1966), new combination; Limbodessus leveri (J. Balfour-Browne, 1944), new combination; and Limbodessus plicatus (Sharp, 1882), new combination.

The fate of newly settled dispersers on freshly colonized oceanic islands is a central theme of island biogeography. The emergence of increasingly sophisticated methods of macroevolutionary pattern inference paves the way for a deeper understanding of the mechanisms governing these diversification patterns on lineages following their colonization of oceanic islands. Here we infer a comprehensive molecular phylogeny for Melanesian Exocelina diving beetles. Recent methods in historical biogeography and diversification rate inference were then used to investigate the evolution of these insects in space and time. An Australian origin in the mid-Miocene was followed by independent colonization events towards New Guinea and New Caledonia in the late Miocene. One colonization of New Guinea led to a large radiation of >150 species and 3 independent colonizations of New Caledonia gave rise to about 40 species. The comparably late colonizations of Vanuatu, Hawaii and China left only one or two species in each region. The contrasting diversification trajectories of these insects on Melanesian islands are likely accounted for by island size, age and availability of ecological opportunities during the colonization stage.

Background: The goal of the present study was to summarize current treatment for acute acromioclavicular (AC) joint dislocations., Method: In all, 796 orthopedics and/or trauma departments in Germany were identified in the national hospital directory (www.dka.de). Using the respective hospital web sites, the email addresses of the responsible shoulder/sports surgeon or, if not available, the head of department (successful in 746 cases) were identified. Emails with a link to an online survey with 36 questions and two reminders were sent; 60 were undeliverable. Thus, 686 emails probably reached the addressee. A total of 203 colleagues (30%) participated in the survey. Findings were compared to results of the survey published by Bäthis et al. in 2001., Results: More than 90% treat Rockwood I or II injuries conservatively and Rockwood IV to VI injuries surgically. However, 34% answered having never seen a Rockwood VI injury. Of those replying, 73% prefer surgical treatment for Rockwood III injuries. Favored surgical techniques were the hook plate in 44% and the arthoscopic TightRope® in 27%, while 11% prefer coracoclavicular cerclage, 6% AC joint transfixation, 3% minimally invasive AC joint reconstruction (MINAR), 1% coracoclavicular screw fixation, and 8% a completely different procedure. Concerning acute Rockwood III injuries, orthopedics and/or trauma surgeons still prefer surgical treatment but less often than 10 years ago (84%)., Conclusions: Favored techniques have completely changed since 2001 when the majority of physicians preferred AC joint transfixation or coracoclavicular cerclages, both techniques that are hardly used today. The hook plate appears to have become "standard therapy". The arthroscopic TightRope® is the second most common despite being the newest technique.

The charismatic tropical Polyura Nawab butterflies are distributed across twelve biodiversity hotspots in the Indomalayan/Australasian archipelago. In this study, we tested an array of species delimitation methods and compared the results to existing morphology-based taxonomy. We sequenced two mitochondrial and two nuclear gene fragments to reconstruct phylogenetic relationships within Polyura using both Bayesian inference and maximum likelihood. Based on this phylogenetic framework, we used the recently introduced bGMYC, BPP and PTP methods to investigate species boundaries. Based on our results, we describe two new species Polyura paulettae Toussaint sp. n. and Polyura smilesi Toussaint sp. n., propose one synonym, and five populations are raised to species status. Most of the newly recognized species are single-island endemics likely resulting from the recent highly complex geological history of the Indomalayan-Australasian archipelago. Surprisingly, we also find two newly recognized species in the Indomalayan region where additional biotic or abiotic factors have fostered speciation. Species delimitation methods were largely congruent and succeeded to cross-validate most extant morphological species. PTP and BPP seem to yield more consistent and robust estimations of species boundaries with respect to morphological characters while bGMYC delivered contrasting results depending on the different gene trees considered. Our findings demonstrate the efficiency of comparative approaches using molecular species delimitation methods on empirical data. They also pave the way for the investigation of less well-known groups to unveil patterns of species richness and catalogue Earth's concealed, therefore unappreciated diversity., (Published by Elsevier Inc.)

Background: Bone tunnel enlargement is a phenomenon present in all anterior cruciate ligament (ACL)- reconstruction techniques. It was hypothesized that press-fit fixation using a free autograft bone plug reduces the overall tunnel size in the tibial tunnel., Methods: In a prospective cohort study twelve patients who underwent primary ACL reconstruction using an autologous quadriceps tendon graft and adding a free bone block for press-fit fixation (PF) in the tibial tunnel were matched to twelve patients who underwent ACL reconstruction with a hamstring graft and interference screw fixation (IF). The diameters of the bone tunnels were analysed by a multiplanar reconstruction technique (MPR) in a CT scan three months postoperatively. Manual and instrumental laxity (Lachman test, Pivot-shift test, Rolimeter) and functional outcome scores (International Knee Documentation Committee sore, Tegner activity level) were measured after one year follow up., Results: In the PF group the mean bone tunnel diameter at the level of the joint entrance was not significantly enlarged. One and two centimeter distal to the bone tunnel diameter was reduced by 15% (p = .001). In the IF group the bone tunnel at the level of the joint entrance was enlarged by 14% (p = .001). One and two centimeter distal to the joint line the IF group showed a widening of the bone tunnel by 21% (p < .001) One and two centimeter below the joint line the bone tunnel was smaller in the PF group when compared to the IF group (p < .001). No significant difference for laxity test and functional outcome scores could be shown., Conclusion: This study demonstrates that press-fit fixation with free autologous bone plugs in the tibial tunnel results in significantly smaller diameter of the tibial tunnel compared to interference screw fixation.

Agaporomorphusjulianeae sp. n. is described from the Biological Field Station Panguana, in Huànuco province of central Peru. The new species belongs to the Agaporomorphusknischi-group sensu Miller 2005. Together with Agaporomorphusknischi Zimmermann, 1921 and Agaporomorphuscolberti Miller & Wheeler, 2008 this is the third species of the genus with broadly enlarged male antennomeres. The new species can be separated from Agaporomorphuscolberti and Agaporomorphusknischi by the smaller please expanded male antennomere VIII, and the form of the median lobe. Important species characters (median lobe, male antennae, metafemur, colour pattern) of the new species and Agaporomorphusknischi are figured, and the habitat, a temporary blackwater forest pond, and its species rich water beetle coenosis are illustrated and described in detail. The Brazilian Agaporomorphusmecolobus Miller, 2001, only known from the type material from Sao Paulo, is here recorded for Minas Gerais. Habitus photos of four additional Agaporomorphus species and Hydrodytesopalinus (Zimmermann, 1921) are provided. Altogether ten species of Agaporomorphus are now known.

Sekaliporus davidi sp. n. is described from northern Australia. It is the second species in the genus. It occurs from the Kimberley region in the northwest of Western Australia to north-eastern Queensland. The new species is morphologically similar to S. kriegi Watts, 1997 described from the Kakadu area in the Northern Territory but well characterized by its constantly larger size, six yellowish markings on elytra, the different form of the apical triangular spine of elytra, and the form of the median lobe. Sekaliporus davidi sp. n. is a lotic species from intermittent creeks and slow flowing rivers and their residual pools. Important species characters (median lobes, parameres and colour patterns) of the two species are figured, and notes on their habitats and distribution are given. Both species are capable of flight and were also collected at light.

Beetles are the most diverse group of animals and are crucial for ecosystem functioning. In many countries, they are well established for environmental impact assessment, but even in the well-studied Central European fauna, species identification can be very difficult. A comprehensive and taxonomically well-curated DNA barcode library could remedy this deficit and could also link hundreds of years of traditional knowledge with next generation sequencing technology. However, such a beetle library is missing to date. This study provides the globally largest DNA barcode reference library for Coleoptera for 15 948 individuals belonging to 3514 well-identified species (53% of the German fauna) with representatives from 97 of 103 families (94%). This study is the first comprehensive regional test of the efficiency of DNA barcoding for beetles with a focus on Germany. Sequences ≥500 bp were recovered from 63% of the specimens analysed (15 948 of 25 294) with short sequences from another 997 specimens. Whereas most specimens (92.2%) could be unambiguously assigned to a single known species by sequence diversity at CO1, 1089 specimens (6.8%) were assigned to more than one Barcode Index Number (BIN), creating 395 BINs which need further study to ascertain if they represent cryptic species, mitochondrial introgression, or simply regional variation in widespread species. We found 409 specimens (2.6%) that shared a BIN assignment with another species, most involving a pair of closely allied species as 43 BINs were involved. Most of these taxa were separated by barcodes although sequence divergences were low. Only 155 specimens (0.97%) show identical or overlapping clusters., (© 2014 John Wiley & Sons Ltd.)

Purpose: The purpose of this study was to determine the inter- and intraobserver variability of intraarticular landmark identification for tunnel position calculation in image-free anterior cruciate ligament (ACL) navigation., Methods: In a test/retest scenario, thirteen experienced ACL surgeons (>50 reconstructions year) experienced in image-free ACL navigation were asked to identify the landmarks required for image-free ACL navigation in the same cadaver knee. Landmark positions were registered using a fluoroscopic ACL navigation system. Positions were determined using validated radiological measurement methods. For variability analysis, mean positions, deviations between the test/retest positions, standard deviations (SD) and range were calculated., Results: Interobserver analysis showed a mean variability (SD) for the tibial landmark positions of 3.0 mm with deviations of up to 24.3 mm (range). Mean femoral landmark variability was 2.9 mm (SD) with deviations of up to 11.3 mm (range). Intraobserver analysis showed a tibial reproducibility of 2.2 mm (SD 2.0 mm; range 10.9 mm) and a femoral of 1.9 mm (SD 1.9 mm; range 10.4 mm)., Conclusion: The data of the presented study suggest that a considerable inter- and intraobserver variability in intraarticular landmark identification exists. Reasonable ranges were found that have to be considered as a potential risk for miscalculation of tunnel positions in image-free ACL reconstruction., Clinical Relevance: Landmark acquisition affects tunnel calculation in image-free ACL., Level of Evidence: IV.

Purpose: The purpose of this study was to evaluate currently preferred treatment strategies as well as the acceptance of new arthroscopic techniques among German orthopaedic surgeons. We assumed that surgeons specialized in shoulder surgery and arthroscopy would treat acute acromioclavicular joint dislocations different to non-specialized surgeons., Methods: Seven hundred and ninety-six orthopaedic and/or trauma departments were found through the German hospital directory of 2012. Corresponding websites were searched for the email address of the chair of shoulder surgery (if applicable) or the department. Seven hundred forty-six emails with the request for study participation including a link to an online survey of 36 questions were sent. In 60 emails, the recipient was unknown., Results: Two hundred and three (30 %) surgeons participated in the survey. one hundred and one were members of the AGA (German-speaking Society for Arthroscopy and Joint-Surgery) and/or of the DVSE (German Association of Shoulder and Elbow Surgery) and regarded as specialists, while 102 were non-members and regarded as non-specialists. According to the treatment of Rockwood I/II and IV-VI injuries, no significant differences were found. Seventy-four % of non-specialists and 67 % of specialists preferred surgical treatment for Rockwood III injuries (P = 0.046). Non-specialists would use the hook plate in 56 % followed by the TightRope in 16 %; specialists would use the TightRope in 38 % followed by the hook plate in 32 % (P = 0.004)., Conclusions: The majority of German orthopaedic and trauma surgeons advise surgical treatment for Rockwood III injuries. Specialists recommend surgery less often. Non-specialists prefer the hook plate, whereas specialists prefer the arthroscopic TightRope technique., Level of Evidence: Observational survey, Level IV.

Anisomeriini diving beetles contain only two enigmatic species, representing a remarkable disjunction between the Pacific Juan Fernández Islands (Anisomeria bistriata) and the South Atlantic Tristan da Cunha Archipelago (Senilites tristanicola). They belong to the Colymbetinae, which contain 140 species worldwide. Here we aim to reconstruct the evolutionary history of the Anisomerinii and use > 9000 bp DNA sequence data from 13 fragments of 12 loci for a comprehensive sampling of Colymbetinae species. Analyses under different optimization criteria converge on very similar topologies, and show unambiguously that Anisomeria bistriata and Senilites tristanicola belong to the Neotropical Rhantus signatus species group, a comparatively recent clade within Colymbetinae. Anisomeriini therefore are synonomized with Colymbetini and both species are transferred to Rhantus accordingly, resulting in secondary homonymy of Rhantus bistriatus (Brullé, 1835) with Rhantus bistriatus (Bergsträsser, 1778). We propose the replacement name Rhantus selkirki Jäch, Balke & Michat nom. nov. for the Juan Fernández species. Presence of these species on remote islands is therefore not relictary, but the result of more recent range expansions out of mainland South America. Finally, we suggest that Carabdytini should be synonymized with Colymbetini. Our study underpins the Hennigian principle that a natural classification can be derived only from the search for shared apomorphies between species, not from differences., (© The Willi Hennig Society 2014.)

Purpose: The quantification of posterior knee laxity is crucial in the evaluation of injuries to the posterior cruciate ligament (PCL) as it has important implications to the treatment for these injuries. The objective of this study was to compare the test results of stress radiography and instrumented drawer testing (Rolimeter) for the assessment of posterior knee laxity in patients with PCL injury., Methods: In 45 patients with an injury to the PCL, lateral radiographs of both knees were obtained under anterior and posterior tibial load (150 N) using a standardized approach. Patients with injury of the ACL to either knee were excluded from the study. During the same clinic visit, an instrumented measurement of anterior-posterior knee laxity was performed at 90° of knee flexion by an experienced examiner using the Rolimeter device. The mean side-to-side differences (SSD) of both (radiographic and instrumented) measurements were compared to each other, and the correlation was calculated using the Pearson coefficient., Results: The SSD (affected/healthy side) of posterior translation determined from the radiographs was 8.2 ± 3.2 mm, and the SSD of the instrumented measurement testing was 7.6 ± 3.2 mm. The mean difference between radiographic and Rolimeter test results was 1.8 ± 1.5 mm. The Pearson's correlation coefficient revealed a good correlation between both measurements of 0.74., Conclusions: It may be suggested by the results of this study that under the absence of an ACL injury and a fixed posterior drawer sign, the Rolimeter is a suitable tool to assess the posterior laxity of the knee in the clinical setting. Its advantages are the absence of radiation to the patient, its availability, and the low cost. Therefore, the Rolimeter device may serve as a valuable alternative to stress radiography in the evaluation of PCL-injured patients., Level of Evidence: II.

Background: The use of interference screws for femoral graft fixation in anterior cruciate ligament (ACL) reconstruction with hamstring grafts can result in rotation of the graft around the screw leading to changes in the final position of the graft within the bone tunnel., Material and Methods: In a prospective study 107 patients (54 right and 53 left knees) underwent ACL reconstruction with a hamstring tendon autograft. Femoral fixation of the graft was performed with a standard right-thread screw in all cases. Patients were assessed at 6 months postoperatively with the international knee documentation committee (IKDC) standard evaluation including instrumented laxity measurements and the results were compared between right and left knees., Results: A significantly higher postoperative anterior laxity was observed in left knees with a negative Lachman test in only 64 % of the cases compared with 87 % in the group of right knees. Accordingly, instrumented laxity measurements of the reconstructed knee compared with the contralateral knee revealed significant differences between left and right knees (left knees 1.8±1.2 mm and right knees 1.0±1.4 mm)., Conclusions: This study demonstrates the importance of femoral graft positioning and its sensitivity to multiple influencing factors. The use of standard right-thread interference screws for femoral graft fixation in the mirrored situation of right and left knees may produce a systematic error in ACL reconstruction. Due to a possible rotation of the graft around the screw, the final position of the transplant may vary thus leading to significant changes in anterior translation of the operated knee.

Purpose: Arthroscopic double-row (DR) suture anchor repair of rotator cuff tears has been shown to be superior to most single-row (SR) techniques with regard to footprint reconstruction, load to failure and tendon-to-bone contact pressures. The hypothesis of this study was that the greater contact pressures of DR techniques would compromise blood flow to a higher degree than SR repair. The aim of this experimental study was to evaluate the effect of a DR and a SR technique on tendon blood flow in a sheep model., Methods: Eighteen sheep underwent detachment and immediate repair of the infraspinatus tendon using either a Mason-Allen or a suture-bridge technique. Tendon blood flow was measured using laser Doppler flowmetry before detachment, immediately after repair and 12 weeks thereafter. Six regions of interest were measured, three over the lateral and three over the medial aspect of the footprint., Results: Immediately after repair, tendon blood flow decreased significantly in both repair groups (P = 0.004). In the SR group, blood flow decreased by 100.1 arbitrary units (AU) (83.6 %) after repair, in the DR by 81.4 AU (90.5 %). Subgroup analysis showed blood flow over the lateral aspect of the footprint decreased by 126.3 AU (92.2 %) in the SR and 84.4 AU (90.9 %) in the DR group, whereas over the medial aspect, it decreased by 73.9 AU (72.0 %) in the SR and 78.5 AU (90.1 %) in the DR group. None of the differences between the groups were significant. At 12 weeks, measured blood flow in the DR group had increased to 90.1 AU (100.2 %) compared to the native tendons and was at 72.5 AU (60.5 %) for the SR group. Again, the difference between SR and DR group was not statistically significant (n.s.). Assessment for retears showed that 4 of 8 tendons (50 %) in the DR group and 3 of 9 tendons (33.3 %) in the SR group had to be classified as re-ruptures., Conclusion: Suture anchor repair leads to an intraoperative decrease in tendon blood flow regardless of the repair technique. A significant difference between SR and DR repair was not found. These findings indicate that tendon blood flow should not be a factor to determine the use of either repair technique over the other.

During the Cenozoic, Australia experienced major climatic shifts that have had dramatic ecological consequences for the modern biota. Mesic tropical ecosystems were progressively restricted to the coasts and replaced by arid-adapted floral and faunal communities. Whilst the role of aridification has been investigated in a wide range of terrestrial lineages, the response of freshwater clades remains poorly investigated. To gain insights into the diversification processes underlying a freshwater radiation, we studied the evolutionary history of the Australasian predaceous diving beetles of the tribe Hydroporini (147 described species). We used an integrative approach including the latest methods in phylogenetics, divergence time estimation, ancestral character state reconstruction, and likelihood-based methods of diversification rate estimation. Phylogenies and dating analyses were reconstructed with molecular data from seven genes (mitochondrial and nuclear) for 117 species (plus 12 outgroups). Robust and well-resolved phylogenies indicate a late Oligocene origin of Australasian Hydroporini. Biogeographic analyses suggest an origin in the East Coast region of Australia, and a dynamic biogeographic scenario implying dispersal events. The group successfully colonized the tropical coastal regions carved by a rampant desertification, and also colonized groundwater ecosystems in Central Australia. Diversification rate analyses suggest that the ongoing aridification of Australia initiated in the Miocene contributed to a major wave of extinctions since the late Pliocene probably attributable to an increasing aridity, range contractions and seasonally disruptions resulting from Quaternary climatic changes. When comparing subterranean and epigean genera, our results show that contrasting mechanisms drove their diversification and therefore current diversity pattern. The Australasian Hydroporini radiation reflects a combination of processes that promoted both diversification, resulting from new ecological opportunities driven by initial aridification, and a subsequent loss of mesic adapted diversity due to increasing aridity., (© The Author(s) 2014. Published by Oxford University Press, on behalf of the Society of Systematic Biologists. All rights reserved. For Permissions, please email: journals.permissions@oup.com.)

Background and Purpose: The purpose of our study was to present the spectrum and prevalence of intraarticular lesions in patients with shoulder pain after a single non-dislocating shoulder trauma and to evaluate the clinical results according to pathology and workers' compensation status., Methods: Sixty consecutive patients (61 shoulders) with shoulder pain following a single non-dislocating shoulder trauma had shoulder arthroscopy. The indication for surgery was either persistent pain for 3 months or longer after trauma and/or an intraarticular lesion on MRI. Patients with history of shoulder complaints, previous shoulder surgery, a complete rotator cuff tear or a fracture of the shoulder girdle were excluded. Intraarticular findings during shoulder arthroscopy were retrospectively analyzed. After a minimum follow-up of 1 year patients were contacted by telephone interview and ASES score, Simple Shoulder Test, Subjective Shoulder Value and residual pain were assessed for the entire population and for patients with and without workers' compensation., Results: The most common intraarticular findings were SLAP (44.3 %) and Pulley (19.7 %) lesions followed by lesions of the anterior or posterior labrum (14.8 %). The mean age of the 13 women and 47 men was 41.9 years (SD 10.9). Patients with workers' compensation had significantly lower scores than patients without and had a significantly lower return to work rate than patients without., Interpretation: In patients with persistent shoulder pain after sprain or contusion arthroscopy revealed a broad spectrum of intraarticular findings. Patients with workers' compensation claims had worse results than those without., Level of Evidence: Case series (Level IV).

Twenty three new species of Exocelina Broun, 1886 from New Guinea are described herein: Exocelinabewaniensis sp. n., Exocelinabismarckensis sp. n., Exocelinacraterensis sp. n., Exocelinagorokaensis sp. n., Exocelinaherowana sp. n., Exocelinajimiensis sp. n., Exocelinakisli sp. n., Exocelinaksionseki sp. n., Exocelinalembena sp. n., Exocelinamantembu sp. n., Exocelinamichaelensis sp. n., Exocelinapinocchio sp. n., Exocelinapseudoastrophallus sp. n., Exocelinapseudobifida sp. n., Exocelinapseudoedeltraudae sp. n., Exocelinapseudoeme sp. n., Exocelinasandaunensis sp. n., Exocelinasimbaiarea sp. n., Exocelinaskalei sp. n., Exocelinatabubilensis sp. n., Exocelinatariensis sp. n., Exocelinavovai sp. n., and Exocelinawannangensis sp. n. All of them have been found to belong to the Exocelinaekari-group. An identification key to all known species of the group is provided, and important diagnostic characters (habitus, color, male antennae, protarsomeres 4-5, median lobes, and parameres) are illustrated. Data on the distribution of the new species and some already described species are given.

The genus Trigonopterus Fauvel, 1862 is highly diverse in Melanesia. Only one species, Trigonopterusamphoralis Marshall, 1925 was so far recorded West of Wallace's Line (Eastern Sumatra). Based on focused field-work the fauna from Sundaland (Sumatra, Java, Bali, Palawan) and the Lesser Sunda Islands (Lombok, Sumbawa, Flores) is here revised. We redescribe Trigonopterusamphoralis Marshall and describe an additional 98 new species: Trigonopterusacuminatus sp. n., Trigonopterusaeneomicans sp. n., Trigonopterusalaspurwensis sp. n., Trigonopterusallopatricus sp. n., Trigonopterusallotopus sp. n., Trigonopterusangulicollis sp. n., Trigonopterusargopurensis sp. n., Trigonopterusarjunensis sp. n., Trigonopterusasper sp. n., Trigonopterusattenboroughi sp. n., Trigonopterusbaliensis sp. n., Trigonopterusbatukarensis sp. n., Trigonopterusbawangensis sp. n., Trigonopterusbinodulus sp. n., Trigonopterusbornensis sp. n., Trigonopteruscahyoi sp. n., Trigonopteruscostipennis sp. n., Trigonopteruscuprescens sp. n., Trigonopteruscupreus sp. n., Trigonopterusdacrycarpi sp. n., Trigonopterusdelapan sp. n., Trigonopterusdentipes sp. n., Trigonopterusdiengensis sp. n., Trigonopterusdimorphus sp. n., Trigonopterusdisruptus sp. n., Trigonopterusdua sp. n., Trigonopterusduabelas sp. n., Trigonopterusechinatus sp. n., Trigonopterusempat sp. n., Trigonopterusenam sp. n., Trigonopterusfissitarsis sp. n., Trigonopterusflorensis sp. n., Trigonopterusfoveatus sp. n., Trigonopterusfulgidus sp. n., Trigonopterusgedensis sp. n., Trigonopterushalimunensis sp. n., Trigonopterushonjensis sp. n., Trigonopterusijensis sp. n., Trigonopterusjavensis sp. n., Trigonopteruskalimantanensis sp. n., Trigonopteruskintamanensis sp. n., Trigonopterusklatakanensis sp. n., Trigonopteruslampungensis sp. n., Trigonopteruslatipes sp. n., Trigonopteruslima sp. n., Trigonopteruslombokensis sp. n., Trigonopterusmerubetirensis sp. n., Trigonopterusmesehensis sp. n., Trigonopterusmicans sp. n., Trigonopterusmisellus sp. n., Trigonopteruspalawanensis sp. n., Trigonopteruspangandaranensis sp. n., Trigonopterusparaflorensis sp. n., Trigonopteruspararugosus sp. n., Trigonopterusparasumbawensis sp. n., Trigonopteruspauxillus sp. n., Trigonopteruspayungensis sp. n., Trigonopterusporcatus sp. n., Trigonopteruspseudoflorensis sp. n., Trigonopteruspseudosumbawensis sp. n., Trigonopteruspunctatoseriatus sp. n., Trigonopterusranakensis sp. n., Trigonopterusrelictus sp. n., Trigonopterusrinjaniensis sp. n., Trigonopterusroensis sp. n., Trigonopterusrugosostriatus sp. n., Trigonopterusrugosus sp. n., Trigonopterusrutengensis sp. n., Trigonopterussaltator sp. n., Trigonopterussantubongensis sp. n., Trigonopterussasak sp. n., Trigonopterussatu sp. n., Trigonopterusschulzi sp. n., Trigonopterussebelas sp. n., Trigonopterussembilan sp. n., Trigonopterussepuluh sp. n., Trigonopterusseriatus sp. n., Trigonopterusserratifemur sp. n., Trigonopterussetifer sp. n., Trigonopterussilvestris sp. n., Trigonopterussingkawangensis sp. n., Trigonopterussingularis sp. n., Trigonopterussinuatus sp. n., Trigonopterussqualidus sp. n., Trigonopterussumatrensis sp. n., Trigonopterussumbawensis sp. n., Trigonopterussundaicus sp. n., Trigonopterussuturalis sp. n., Trigonopterussyarbis sp. n., Trigonopterustelagensis sp. n., Trigonopterustepalensis sp. n., Trigonopterustiga sp. n., Trigonopterustrigonopterus sp. n., Trigonopterustujuh sp. n., Trigonopterusujungkulonensis sp. n., Trigonopterusvariolosus sp. n., Trigonopterusvulcanicus sp. n., Trigonopteruswallacei sp. n.. All new species are authored by the taxonomist-in-charge, Alexander Riedel. Most species belong to the litter fauna of primary wet evergreen forests. This habitat has become highly fragmented in the study area and many of its remnants harbor endemic species. Conservation measures should be intensified, especially in smaller and less famous sites to minimize the number of species threatened by extinction.

Introduction: Shoulder dislocations can cause acute and chronic instabilities that need to be addressed in order to restore joint functioning. The transfer of the coracoid process has become a feasible surgical procedure in patients with shoulder instability. Several concomitant injuries after recurrent dislocations have been described., Case Presentation: A 32-year-old German man presented to our department with a history of recurrent shoulder dislocations. He was diagnosed with an avulsion fracture of the coracoid process and dislocation of an osseous piece with attachment to the conjoined tendons during the surgical transfer of the coracoid process. Therefore, we performed an open Latarjet procedure and reattached the bony piece with the conjoined tendons to the glenoid rim. Three months after the operation the patient presented with a satisfying range of motion and without instabilities or pain. He was able to return to his job., Conclusions: Patients suffering from anterior shoulder dislocation might develop accompanying lesions after numerous dislocations that are not present upon first visit. Different techniques for the reconstruction of the glenoid rim and the restoration of shoulder joint stability have been described in the literature. We opted for a coracoid transfer and achieved an optimal reconstruction, as shown on the postoperative computed tomography scan. An avulsion fracture of the coracoid process with dislocation of the conjoined tendons can not only be found in patients suffering a direct trauma as pointed out in the literature, but also in patients with anterior shoulder instability with recurrent anterior shoulder dislocation.

The second- and third instar larvae of the cliff water beetle Aspidytes wrasei Balke, Ribera & Beutel, 2003 are studied for the first time with special emphasis on morphometry and chaetotaxy. A review of the characters useful in the identification of larvae of Aspidytes Ribera, Beutel, Balke & Vogler, 2002 is presented. Confirming previous findings, larvae of this genus are unique within Hydradephaga in the dorsally oriented spiracles on the abdominal segment VIII of instars II and III. The inclusion of Aspidytidae within the superfamily Dytiscoidea is reinforced by the following putative synapomorphies: presence of pore PAp, proximal insertion of pore ANg, apical or subapical insertion of seta MX8, presence of pore LAd, and distal insertion of seta CO6. Larvae of A. wrasei differ from those of A. niobe Ribera, Beutel, Balke & Vogler, 2002 in several significant characters that may indicate that both species have a long history of independent evolution.

Macroinvertebrates that are collected in large numbers pose major problems in basic and applied biodiversity research: identification to species via morphology is often difficult, slow and/or expensive. DNA barcodes are an attractive alternative or complementary source of information. Unfortunately, obtaining DNA barcodes from specimens requires many steps and thus time and money. Here, we promote a short cut to DNA barcoding, that is, a nondestructive PCR method that skips DNA extraction ('direct PCR') and that can be used for a broad range of invertebrate taxa. We demonstrate how direct PCR can be optimized for the larvae and adults of nonbiting midges (Diptera: Chironomidae), a typical invertebrate group that is abundant, contains important bioindicator species, but is difficult to identify based on morphological features. After optimization, direct PCR yields high PCR success rates (>90%), preserves delicate morphological features (e.g. details of genitalia, and larval head capsules) while allowing for the recovery of genomic DNA. We also document that direct PCR can be successfully optimized for a wide range of other invertebrate taxa that need routine barcoding (flies: Culicidae, Drosophilidae, Dolichopodidae, Sepsidae; sea stars: Oreasteridae). Key for obtaining high PCR success rates is optimizing (i) tissue quantity, (ii) body part, (iii) primer pair and (iv) type of Taq polymerase. Unfortunately, not all invertebrates appear suitable because direct PCR has low success rates for other taxa that were tested (e.g. Coleoptera: Dytiscidae, Copepoda, Hymenoptera: Formicidae and Odonata). It appears that the technique is less successful for heavily sclerotized insects and/or those with many exocrine glands., (© 2014 John Wiley & Sons Ltd.)

Introduction: Since tunnel positioning is one of the key factors in anterior cruciate ligament (ACL) reconstruction and the variability of tunnel positioning in ACL reconstruction has so far never been analyzed, the objective of this study was to determine the inter- and intra-observer variability of tibial and femoral tunnel positioning in ACL reconstruction., Materials and Methods: In an operating room setup, 13 surgeons were asked to identify the tunnel positions in one and the same ACL-deficient cadaver knee. Using a fluoroscopic based ACL navigation system, tunnel positions were digitally measured in a test/re-test scenario. For variability analysis mean positions, standard deviations and range were calculated as well as differences between test/re-test positions., Results: The intraobserver analysis showed a tibial variability of 3.3 mm (SD 2.1, range 7.5 mm) and a femoral variability of 2.0 mm (SD 1.6 mm, range 6.8 mm). The interobserver variability of the tibial tunnel positions was 3.2 mm (SD) with a range of 18.3 mm and a femoral variability of 3.7 mm (SD) with a range of 13.2 mm., Conclusions: This study demonstrates that a reasonable inter- and intra-observer variability in ACL tunnel positioning exists even among experienced surgeons. Although deviations of 2-3 mm may seem to be acceptable at first sight, a range of up to 18.3 mm indicates that outliers exist, which can cause graft failure. More reliable reconstruction techniques should be developed to reduce the variability in tunnel positioning.

Background: The use of interference screws for femoral graft fixation in anterior cruciate ligament (ACL) reconstruction with hamstring grafts can result in rotation of the graft around the screw leading to changes in the final position of the graft within the bone tunnel., Material and Methods: In a prospective study 107 patients (54 right and 53 left knees) underwent ACL reconstruction with a hamstring tendon autograft. Femoral fixation of the graft was performed with a standard right-thread screw in all cases. Patients were assessed at 6 months postoperatively with the international knee documentation committee (IKDC) standard evaluation including instrumented laxity measurements and the results were compared between right and left knees., Results: A significantly higher postoperative anterior laxity was observed in left knees with a negative Lachman test in only 64 % of the cases compared with 87 % in the group of right knees. Accordingly, instrumented laxity measurements of the reconstructed knee compared with the contralateral knee revealed significant differences between left and right knees (left knees 1.8±1.2 mm and right knees 1.0±1.4 mm), Conclusions: This study demonstrates the importance of femoral graft positioning and its sensitivity to multiple influencing factors. The use of standard right-thread interference screws for femoral graft fixation in the mirrored situation of right and left knees may produce a systematic error in ACL reconstruction. Due to a possible rotation of the graft around the screw, the final position of the transplant may vary thus leading to significant changes in anterior translation of the operated knee.

Early studies on Melanesian mountain systems provided insights for fundamental evolutionary and ecological concepts. These island-like systems are thought to provide opportunities in the form of newly formed, competition-free niches. Here we show that a hyperdiverse radiation of freshwater arthropods originated in the emerging central New Guinea orogen, out of Australia, about 10 million years ago. Further diversification was mainly allopatric, with repeated more recent colonization of lowlands as they emerged in the form of colliding oceanic island arcs, continental fragments and the Papuan Peninsula, as well as recolonization of the central orogen. We unveil a constant and ongoing process of lineage accumulation while the carrying capacity of the island is about to be reached, suggesting that lineage diversification speed now exceeds that of landmass/new ecological opportunity formation. Therefore, the central orogeny of New Guinea acts as a motor of diversification for the entire region.