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202. A High-Resolution 15,000Rad Radiation Hybrid Panel for the Domestic Cat.

Author

Bach, L. H., Gandolfi, B., Grahn, J .C., Millon, L. V., Kent, M. S., Narfstrom, K., Cole, S. A., Mullikin, J. C., Grahn, R. A., and Lyons, L. A.

Subjects
*GENOMES, *FIBROBLASTS, *ABYSSINIAN cat, *CELL lines, *BIOMARKERS
Abstract

The current genetic and recombination maps of the cat have fewer than 3,000 markers and a resolution limit greater than 1 Mb. To complement the first-generation domestic cat maps, support higher resolution mapping studies, and aid genome assembly in specific areas as well as in the whole genome, a 15,000Rad radiation hybrid (RH) panel for the domestic cat was generated. Fibroblasts from the female Abyssinian cat that was used to generate the cat genomic sequence were fused to a Chinese hamster cell line (A23), producing 150hybridlines. The clones were initially characterized using 39 short tandem repeats (STRs) and 1,536 SNP markers. The utility of whole-genome amplification in preserving and extending RH panel DNA was also tested using 10 STR markers; no significant difference in retention was observed. The resolution of the 15,000Rad RH panel was established by con structing framework maps across 10 different 1-Mb regions on different feline chromosomes. In these regions, 2-point analysis was used to estimate RH distances, which compared favorably with the estimation of physical distances. The study demonstrates that the 15,000Rad RH panel constitutes a powerful tool for constructing high-resolution maps, having an average resolution of 40.1 kb per marker across the ten 1-Mb regions. In addition, the RH panel will complement existing genomic resources for the domestic cat, aid in the accurate re-assemblies of the forthcoming cat genomic sequence, and support cross-species genomic comparisons. [ABSTRACT FROM AUTHOR]

Published
2012
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