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Background: Ultrasound has shown promise as visual feedback in remediation of /ɹ/.Aims: To compare treatment for /ɹ/ with and without ultrasound.Methods & Procedures: A Canadian English‐speaking adolescent participated in a case study with a no treatment baseline, alternating treatment blocks with and without ultrasound and a final no treatment period.Outcomes & Results: Formant values and trained listener ratings of speech samples indicated improvement in /ɹ/ production, particularly after the introduction of ultrasound.Conclusions: Ultrasound appeared to facilitate the acquisition of /ɹ/ for the participant. Large‐scale studies are needed to evaluate ultrasound further.

We surveyed little-known ray-finned fish assemblages from Maryland's coastal bays in order to establish species-habitat relationships for common species. From 1996–1999, 25 sites were sampled monthly with otter trawls in the coastal bays of Maryland. Anchoa mitchilli (bay anchovy) constituted nearly 50% of the catch for each year, and species composition was largely similar across years, with some differences likely related to variation in recruitment. For example, Clupea harengus (Atlantic herring) was particularly abundant during 1996 and 1999 following their spawning season. We used canonical correspondence analysis to determine how assemblages were related to temperature, dissolved oxygen (DO), salinity, and land-use variables during summer (June–September) and throughout the rest of the year. A gradient correlated with temperature and DO significantly structured assemblages throughout most of the year; during summer, the proportion of wetland habitat was important. We demonstrate that enviro...

The selective serotonin reuptake inhibitors (SSRIs) are extensively used for the treatment of multiple psychiatric conditions. In vitro and ex vivo data with these agents indicate they may have varying degrees of antiplatelet activity via multiple receptors. Reports of bleeding in patients receiving SSRIs appeared soon after their introduction. A review of the literature suggests SSRI therapy may increase the risk of bleeding especially with concomitant aspirin or nonsteroidal anti-inflammatory agents. Clinicians should exercise caution when prescribing these agents in high risk patients and maintain awareness of the potential contribution of SSRIs to unexplained bleeding episodes.

Common material properties (e.g. hardness, tensile modulus and strength, poisson's ratio) are well known. On the other hand, properties of thin layers and micro volumina can differ significant from those data, in particular for micro/nano-particle systems. AFM and FFM methods work with excellent lateral, topographic and force resolution but often do not provide testing-situations close to practical applications, e.g. high pressure flat contact within micro wear and micro friction investigations. Optimization of friction/wear-properties of multi micro-area contact especially of relevance on curved and topographically structured surfaces need access to measurement technique, that covers the gap between micro and nano scale [3]. The results presented here are done with the UST (Universal Surface Tester) [4] to determine the influence of incorporation of micro/nano-particles and their distribution on micro mechanical and functional properties of polymer materials close to the surface.

The growing accumulation of knowledge about fall prevention strategies primarily reflects a research perspective where intervention data are rigorously collected and analyzed. However, the voices of program deliverers or participants who are part of these interventions are often aggregated and thus muted. With recognition of the growing importance of patient-centered care, we wanted to provide a personal reflection on the Balance Partner program, a CDC funded project to train peer leaders in fall prevention. In the story below, Sara was trained as a Balance Partner using a curriculum developed at The University of North Carolina’s Center for Health Promotion and Disease Prevention. The Balance Partner Program provides training and support to community volunteers who are paired with a peer who is at risk for falls. Together, Balance Partners plan strategies to decrease the likelihood of a fall – strategies that could include joining a balance exercise program, improving home safety, or getting a vision check-up. By addressing social and emotional factors alongside knowledge about falls, the Balance Partner Program aims to increase older adults’ overall adherence to fall prevention activities above the 50% rate reported in literature (1). Sara, a 69-year-old volunteer, was paired with Georgia, who screened at high risk for falls during a Building Better Balance Screening in Asheville, NC, USA. As of this writing, Sara and Georgia had worked together for almost 6 months to implement strategies to reduce their falls risk.

Arabic, a Semitic language of the Afro-Asiatic variety, has a rich consonant inventory. Previous studies on Arabic phonological acquisition have focused primarily on dialects in Jordan and Egypt. Because Arabic varies considerably across regions, information is also needed for other dialects.To determine acquisition benchmarks for singleton consonants for Kuwaiti Arabic-speaking 4-year-olds.Participants were 80 monolingual Kuwaiti Arabic-speaking children divided into two age groups: 46-54 and 55-62 months. Post-hoc, eight children were identified as possibly at risk for protracted phonological development. A native Kuwaiti Arabic speaker audio-recorded and transcribed single-word speech samples (88 words) that tested consonants across word positions within a variety of word lengths and structures. Transcription reliability (point-to-point) was 95% amongst the authors, and 87% with an external consultant. Three acquisition levels were designated that indicated the proportion of children with no mismatches ('errors') for a given consonant: 90%+ of children, 75-89%, fewer than 75%. Mismatch patterns were described in terms of a phonological feature framework previously described in the literature.The Kuwaiti 4-year-olds produced many singleton consonants accurately, including pharyngeals and uvulars. Although the older age group had fewer manner and laryngeal mismatches than the younger age group, consonants still developing at age 5 included coronal fricatives and affricates, trilled /r/ and some uvularized consonants ('emphatics'). The possible at-risk group showed mastery of fewer consonants than the other children. By feature category, place mismatches were the most common, primarily de-emphasis and lack of contrast for [coronal, grooved] (distinguishing alveolar from interdental fricatives). Manner mismatches were next most common: the most frequent substitutions were [+lateral] [l] or other rhotics for /r/, and stops for fricatives. Laryngeal mismatches were few, and involved partial or full devoicing. Group differences generally reflected proportions of mismatches rather than types.Compared with studies for Jordanian and Egyptian Arabic, Kuwaiti 4-year-olds showed a somewhat more advanced consonant inventory than same age peers, especially with respect to uvulars, pharyngeals and uvularized (emphatic) consonants. Similar to the other studies, consonant categories yet to master were: [+trilled] /r/, coronal fricative feature [grooved], [+voiced] fricatives /ʕ, z/ and the affricate /d͡͡ʒ/ and some emphatics. Common mismatch patterns generally accorded with previous studies. This study provides criterion reference benchmarks for Kuwaiti Arabic consonant singleton acquisition in 4-year-olds.

ATR is an attractive target in cancer therapy because it signals replication stress and DNA lesions for repair and to S/G2 checkpoints. Cancer-specific defects in the DNA damage response (DDR) may render cancer cells vulnerable to ATR inhibition alone. We determined the cytotoxicity of the ATR inhibitor VE-821 in isogenically matched cells with DDR imbalance. Cell cycle arrest, DNA damage accumulation and repair were determined following VE-821 exposure. Defects in homologous recombination repair (HRR: ATM, BRCA2 and XRCC3) and base excision repair (BER: XRCC1) conferred sensitivity to VE-821. Surprisingly, the loss of different components of the trimeric non-homologous end-joining (NHEJ) protein DNA-PK had opposing effects. Loss of the DNA-binding component, Ku80, caused hypersensitivity to VE-821, but loss of its partner catalytic subunit, DNA-PKcs, did not. Unexpectedly, VE-821 was particularly cytotoxic to human and hamster cells expressing high levels of DNA-PKcs. High DNA-PKcs was associated with replicative stress and activation of the DDR. VE-821 suppressed HRR, determined by RAD51 focus formation, to a greater extent in cells with high DNA-PKcs. Defects in HRR and BER and high DNA-PKcs expression, that are common in cancer, confer sensitivity to ATR inhibitor monotherapy and may be developed as predictive biomarkers for personalised medicine.

The MYC (v-myc avian myelocytomatosis viral oncogene homolog; c-MYC) locus on chromosome 8q is susceptible to high-level amplification following exposure of human breast cells to ionizing radiation, and c-MYC amplification is a common feature of both radiogenic adenocarcinoma and radiogenic angiosarcoma of the breast. Taken together, these observations suggest common breast-specific susceptibility factors that predispose cells to amplification of this critical proto-oncogene and the development of radiogenic cancer in multiple tissue types of this radiosensitive organ.

Due to the intermittent nature of most renewable energy sources, such as solar and wind, energy storage is increasingly required. Since electricity is difficult to store, hydrogen obtained by electrochemical water splitting has been proposed as an energy carrier. However, the handling and transportation of hydrogen in large quantities is in itself a challenge. We therefore present here a method for hydrogen storage based on a CO2 (HCO3 (-) )/H2 and formate equilibrium. This amine-free and efficient reversible system (>90 % yield in both directions) is catalyzed by well-defined and commercially available Ru pincer complexes. The formate dehydrogenation was triggered by simple pressure swing without requiring external pH control or the change of either the solvent or the catalyst. Up to six hydrogenation-dehydrogenation cycles were performed and the catalyst performance remained steady with high selectivity (CO free H2 /CO2 mixture was produced).

Heavy-ion collisions at lower energies provide a rich environment for investigating reaction dynamics. Recent theory has suggested a sensitivity to the symmetry energy and the equation of state via deformations of the reaction system and ternary breaking of the deformed reaction partners into three heavy fragments. A new detection system has been commissioned at Texas A&M University in an attempt to investigate some of the observables sensitive to the nuclear equation of state.

Atlantic menhaden (Brevoortia tyrannus) is well known for its commercial and ecological importance and has been historically declining in the Chesapeake Bay (Maryland), one of its principal nursery habitats along the eastern coast. Using data from the Striped Bass Seine Survey of the Maryland Department of Natural Resources (2003), we evaluated how the distribution of Atlantic menhaden has changed from 1966 to 2004 for 12 river drainages. We observed significant or marginally significant declines in 42% of the drainages, with drainages of the northern Bay showing the majority of those declines. Continued recruitment to several drainages of the Bay may partly explain why the adult spawning population is not declining. We determined if temporal changes in abundance were related to changes in salinity or water quality for five major drainages of the watershed. For one of these drainages, the Patuxent River, differences in productivity across sites largely explained differences in abundance. For the four remaining drainages, differences in recruitment could not be explained by productivity or salinity gradients. While reducing nitrogen loading and enhancing water clarity may improve Atlantic menhaden production, we suggest that the role of offshore processes on large-scale declines has been largely neglected and studies on larval ingression are necessary for further elucidation of spatial and temporal patterns of juvenile distribution in the Chesapeake Bay.

Normal circadian variations in vasoactive intestinal polypeptide, somatostatin, cholecystokinin and pancreatic polypeptide were measured to determine if these alter with aging and to identify gastrointestinal regulatory hormones that might control the dramatic diurnal variation in the gastric cytoprotective trefoil protein TFF2. Plasma pancreatic polypeptide concentrations showed a marked diurnal rhythm (p < 0.0001). Basal and postprandial pancreatic polypeptide concentrations increased with age (p < 0.01). The timing of the diurnal rhythm was consistent with pancreatic polypeptide inhibiting TFF2 secretion and there was a negative association between pancreatic polypeptide and TFF2 concentrations (p < 0.002). The much higher pancreatic polypeptide concentrations in older people will induce increased satiety that may contribute to 'anorexia of ageing'. These results identify potential therapies for treatment of gastric mucosal morbidity and age-associated loss of appetite.

Expert scientific inquiry involves the generation and use of analogies. How and when students might develop this aspect of expertise has implications for understanding how and when instruction might facilitate that development. In a study of K-8 student inquiry in physical science, we are examining cases of spontaneous analogy generation. In the case we present here, a third-grader generates an analogy and modifies it to reconcile his classmates' counterarguments, allowing us to identify in these third-graders specific aspects of nascent expertise in analogy use. Promoting abilities and inclinations such as these children display requires that educators recognize and respond to them. © 2006 Wiley Periodicals, Inc. Sci Ed90:316–330, 2006

Purpose This clinical focus article describes an exploratory case study addressing reduction of vocal loudness in a young adult with a history of autism spectrum disorder and developmental delay. The need for a short-term pullout individual intervention arose from his participation in the Advancing Language and Literacy group, a program that provides support for enhancement of speech, language, and literacy skills for young adults with developmental disabilities. Method The participant attended individual treatment sessions weekly for 9 sessions, all of which were digitally audio-recorded. Client awareness of different loudness levels was first established using nonspeech sounds and speech samples. Loudness in spontaneous speech was monitored with support of verbal and visual feedback in the individual sessions and tracked in individual sessions as well as sessions of the Advancing Language and Literacy group, which served as a generalization context. Results In the individual sessions, the participant's spontaneous production of acceptable volume improved from 42% to 92% of utterances. Observation of group participation indicated generalization, with the majority of utterances produced posttreatment at an acceptable volume. Conclusions This clinical focus article emphasizes suprasegmental aspects as a dimension of communicative competence and highlights the need for continued intervention research and services for young adults with developmental disabilities.

The present review will include the mammalian trefoil factors, TFF1, TFF2 and TFF3. It will summarise the amino acid sequences from different species, their posttranslational modifications and their structures determined by X-ray analysis and nuclear magnetic resonance studies. Trefoil factors all have a well-defined, structurally conserved trefoil domain. The trefoil domain consists of 42 or 43 amino acid residues and contains 6 cysteine residues that form disulphide bonds in a 1-5, 2-4 and 3-6 configuration. By the establishment of an additional intra-molecular disulphide bond at the C-terminal end, TFF1 and TFF3 form homodimers or heterodimers. This dimer formation of TFF1 and TFF3 will be discussed, and the possible implications for biological activity will be reviewed. The physicochemical characteristics including protease stability of trefoil factors will be summarised. The biological implications of different molecular forms of trefoil factors and their interaction with mucins will be discussed together with other functional insights.

Previous research on proteins that inhibit kidney stone formation has identified a relatively small number of well-characterized inhibitors. Identification of additional stone inhibitors would increase understanding of the pathogenesis and pathophysiology of nephrolithiasis. We have combined conventional biochemical methods with recent advances in mass spectrometry (MS) to identify a novel calcium oxalate (CaOx) crystal growth inhibitor in normal human urine. Anionic proteins were isolated by DEAE adsorption and separated by HiLoad 16/60 Superdex 75 gel filtration. A fraction with potent inhibitory activity against CaOx crystal growth was isolated and purified by anion exchange chromatography. The protein in 2 subfractions that retained inhibitory activity was identified by matrix-assisted laser desorption/ionization-time-of-flight MS and electrospray ionization-quadrupole-time-of-flight tandem MS as human trefoil factor 1 (TFF1). Western blot analysis confirmed the mass spectrometric protein identification. Functional studies of urinary TFF1 demonstrated that its inhibitory potency was similar to that of nephrocalcin. The inhibitory activity of urinary TFF1 was dose dependent and was inhibited by TFF1 antisera. Anti-C-terminal antibody was particularly effective, consistent with our proposed model in which the 4 C-terminal glutamic residues of TFF1 interact with calcium ions to prevent CaOx crystal growth. Concentrations and relative amounts of TFF1 in the urine of patients with idiopathic CaOx kidney stone were significantly less (2.5-fold for the concentrations and 5- to 22-fold for the relative amounts) than those found in controls. These data indicate that TFF1 is a novel potent CaOx crystal growth inhibitor with a potential pathophysiological role in nephrolithiasis.

Summary Objective: To review the aetiologies and preventative methods associated with Jarisch–Herxheimer reactions (JHR). Data sources: Ovid Medline® (1966–June Week 1 2004) was utilized to assess biomedical literature; a review of the bibliographies of articles was also performed. Data synthesis: JHR often occurs with the treatment of spirochete infections. However, the mechanism by which the reaction takes place is not clearly defined. Conclusion: Studies suggest with conflicting evidence that the JHR is caused by release of endotoxin-like material from the spirochete as well as cytokine elevation in the body. It appears the type of drug and the rate of spirochete clearance from the body have little effect on the incidence of the reaction. Many pretreatment options have been explored with limited efficacy with the exception of anti-tumour necrosis factor antibodies.

The pharmacokinetics of oxytetracycline in 2-yr-old loggerhead sea turtles (Caretta caretta) after single i.v. and i.m. injections were studied for biologic marking and therapeutic applications. Twenty juvenile turtles were divided into two treatment groups. Ten animals received 25 mg/kg of oxytetracycline i.v. and 10 received the same dosage i.m. Plasma oxytetracycline concentrations were analyzed by reverse-phase high-performance liquid chromatography. Data from the i.v. route best fit a three-compartment model, whereas noncompartmental analysis was used to compare data from both the i.v. and i.m routes. For the i.v. route, means for maximum plasma concentration, terminal phase half-life, systemic clearance, and apparent volume of distribution at steady state were 6.6 microg/ml, 66.1 hr, 290.7 ml/hr/kg, and 18.4 L, respectively. For the i.m. route, means for systemic availability, maximum plasma concentration, and elimination half-life were 91.8%, 1.6 microg/ml, and 61.9 hr, respectively. The remarkably high apparent volume of distribution may possibly be associated with a deep compartment of drug disposition such as bone deposition associated with the large skeletal mass of turtles and the fact that these were well-nourished, growing juveniles. Although maximum plasma concentration by i.m. administration was lower than for the i.v. route, the long elimination time indicates that an infrequent dosing interval may be effective for sensitive bacteria.

Background/Purpose Although the pathogenesis of necrotizing enterocolitis (NEC) is not completely defined, ischemia appears to be one of the most important causative factors. Trefoil factor family peptide 3 (TFF3) is a peptide normally expressed in the small bowel and colon and is involved in the maintenance and repair of mucosal integrity. The authors hypothesized that monomeric (TFF3 Ser 57 ) and dimeric (TFF3 Cys 57 ) recombinant TFF3 may prevent the development and accelerate healing of intestinal ischemia-reperfusion injury in weanling rats. Methods Intestinal injury was induced in 18-day-old rats by occlusion of the superior mesenteric vessels for 60 minutes. To examine the protective effect, rats were given 3 μg/g of TFF3 Ser 57 or TFF3 Cys 57 by subcutaneous or enteral administration 30 minutes before the vascular occlusion. To examine the healing effect, rats were given 3 μg/g of TFF3 Ser 57 or TFF3 Cys 57 by subcutaneous or enteral administration 60 minutes after the beginning of reperfusion. Samples from small bowel and colon were collected for morphometric analysis after 3 hours of reperfusion. Mucosal damage was assessed by the Chiu score. Results Both forms of TFF3 reduced the amount of damage when administered before the ischemia. Administration of TFF3 Ser 57 and TFF3 Cys 57 after the beginning of reperfusion significantly increased the villous height and decreased the Chiu score in the small intestine and colon. Conclusions TFF3 Ser 57 monomer and TFF3 Cys 57 dimer prevent the development and promote healing of ischemia-reperfusion injury in weanling rats. There are no differences between the routes of administration of TFF3.

The trefoil protein TFF1 is expressed principally in the superficial cells of the gastric mucosa. It is a small protein and forms homo- and hetero-dimers via a disulphide bond through Cys58 which is located three amino acids from the C terminus. TFF1 is co-expressed with the secreted mucin MUC5AC in superficial cells of the gastric mucosa suggesting that it could be involved in the packaging or function of gastric mucus. We have previously shown that TFF1 co-sediments with mucin glycoproteins on caesium chloride gradients. To extend this observation we have now used gel filtration under physiological conditions, immunoprecipitation and Western transfer analysis to characterise the interaction of TFF1 with gastric mucin glycoproteins. We show that TFF1 co-elutes with MUC5AC but not MUC6 on gel filtration and that immunoprecipitation and Western transfer analysis confirms that TFF1 interacts with MUC5AC. We also demonstrate that the TFF1 dimer is the predominant molecular form bound to MUC5AC. Salt and chelators of divalent cations such as EDTA and EGTA disrupted the TFF1- MUC5AC interaction and increased the degradation of MUC5AC, whereas calcium increased the amount of TFF1 bound to MUC5AC. These data support the contention that TFF1 is pivotal in the packaging and function of human gastric mucosa.

BACKGROUND: Post-mortem studies with brain samples of schizophrenic patients led revealed altered GABA-ergic markers like reduced expression of the GABA transporter GAT-1. Whether this effect is due to the pathophysiology of schizophrenia or to antipsychotic treatment has not been investigated. We therefore established an animal trial of long-term antipsychotic treatment to address this question. METHODS: A total of 33 adult male rats were investigated in three cohorts of 11 animals. One group received clozapine (45 mg/kg/ day), another group haloperidol (1.5 mg/kg/day), and the third one pH-adapted water over a period of 6 months. In situ hybridization with cRNA probes specific for GABA transporters VGAT, GAT-1 and GAT-3 were performed in comparison to control animals. RESULTS: While GAT-1 was upregulated, VGAT expression declined in cortical and limbic brain regions, whereby haloperidol showed a greater effect than clozapine. GAT-3 expression was suppressed in parietal and temporal cortex. CONCLUSIONS: We thus conclude that long-term antipsychotic treatment alters GABA transporter expression in rat. The upregulation of GAT-1 contrasts with the post-mortem finding of reduced GAT-1 expression in schizophrenic patients. Our results facilitate the distinction between disease dependent changes of GABAergic markers and medication effects.

Why Helicobacter pylori colonizes only gastric tissue is unknown. It is found on gastric mucus-secreting cells and in the overlying gastric mucus but not deep in gastric glands. This localization mirrors the expression of trefoil factor 1, TFF1. We hypothesized that H. pylori interacting with TFF1 could explain the tropism of this bacteria for gastric tissue. Recombinant human TFF1 expressed in Escherichia coli was purified by affinity chromatography, ion-exchange chromatography, and gel filtration. Binding of H. pylori was assessed by using flow cytometry and the BIAcore system, which allows real-time monitoring of molecular interactions. In flow cytometry, H. pylori bound to the TFF1 dimer, but Campylobacter jejuni strains and the laboratory strain of E. coli , HB101, did not bind. When the BIAcore system was used, H. pylori bound strongly to TFF1-coated dextran chips compared with uncoated chips. Binding was inhibited by a TFF1 monoclonal antibody and by soluble TFF1. H. pylori bound to porcine gastric mucin only if it was pretreated with TFF1. In conclusion, H. pylori interacts avidly with the dimeric form of TFF1, and this interaction enables binding to gastric mucin, suggesting that TFF1 may act as a receptor for the organism in vivo . This interaction may underline the previously unexplained tropism of this organism for gastric tissue and its colocalization with the gastric mucin MUC5AC.

The expression of TFF2 in breast cancer cells and the effect of recombinant TFF2 on breast cancer cell migration were assessed. TFF2 expression was detected by PCR in estrogen receptor-negative and at lower levels in estrogen receptor-positive breast cancer cells. TFF2 expression was detected in nine out of 10 primary breast tumors but its expression was not related to that of the estrogen receptor. Focal expression was observed in normal and tumor cells by immunohistochemistry. TFF2 stimulated the migration of estrogen-responsive MCF-7 and non-responsive MDA-MB231 cells. We conclude that TFF2 is expressed in normal and malignant breast epithelial cells and that it stimulates the migration of breast cancer cells.

Post-mortem-Untersuchungen bestatigten, dass glutamaterge NMDA-, AMPA- und Kainatrezeptoren an der Pathophysiologie der Schizophrenie beteiligt sind. Ob die veranderten Rezeptorzahlen dabei krankheits- oder medikamentenbedingt sind, ist bislang unklar. Im Tiermodell wurde deshalb der Einfluss von antipsychotischer Medikation nach bis zu 6-monatiger Behandlung untersucht und hier zusammengefasst. Ubereinstimmend ergab sich eine erhohte NMDA-Rezeptor-Bindung nach Haloperidol im Striatum und Nucleus accumbens, nach Clozapin nur im Nucleus accumbens. Die AMPA-Rezeptorzahl war nach Haloperidol im Gyrus cinguli, Striatum, insularen Kortex sowie Nucleus accumbens, nach Clozapin im anterioren Gyrus cinguli und infralimbischen Kortex erhoht. Die Kainatrezeptorbindung wurde im Hippokampus von beiden Antipsychotika erhoht, jedoch in groserem Ausmas von Clozapin. Die Ergebnisse zeigen einen differenziellen Effekt zwischen dem Neuroleptikum Haloperidol und dem Atypikum Clozapin. Ein Teil der post-mortem erhobenen Befunde im glutamatergen System bei Patienten mit langjahriger Schizophrenie lassen sich auf auf Medikamenteneffekte zuruckfuhren und sind Ausdruck plastischer Veranderungen durch die Langzeitmedikation mit Antipsychotika.

Over the last decade, much attention has focused on the reorganization of the roles and functions of public health to improve the health of communities. The Pennsylvania State Health Improvement Plan (SHIP) offers one example of how national, state, and local components of the public health system can effectively integrate their strategies and resources to improve responsiveness to local public health needs. This article reviews the policy action and planning process used to develop a community partnership, shared-responsibility model and the strategies and implementation plans that have been adopted to achieve substantial, measurable improvement in community health status.

The invasion of sewer pipes by tree roots is a major cost to both the corporations managing urban infrastructure and to private landowners. There are a number of factors that may result in the growth of roots into and around the pipes. These include pipe material factors such as degradation of aging pipes and damage caused to pipes and environmental factors such as site conditions, tree proximity and tree species. Research into these issues will help in developing more efficient methods of controlling root invasion, benefiting both the management of sewer flow and the urban landscape. A study of root invasions in suburban Melbourne was carried out to examine relationships between site factors and root blockages. Common factors found associated with tree root blockages were Eucalyptus and Melaleuca species over 4m high within 6 m of sewers, pipe depth 2 m or less, rubber ring joined 150 mm diameter vitreous clay, 30 to 60 years old, soil types with sandy topsoils, and blockages occurring most frequently when temperatures and evaporation were at their lowest i.e. August to October. An experiment was conducted to evaluate chemical and physical treatments for preventing root growth in disturbed soil with high nutrient content and readily available moisture (modeling leaking sewers). Over a period of 177 days significant inhibition was achieved with the chemical treatments dichlobenil (278 g/m and 1392 g/m), copper sulphate (1.5 g Cu/kg soil and 7.5 gCu/kg soil), oryzalin (1031 g/m) and trifluralin (260 g/m) and the physical treatments slaked lime (ratio of 10 soil:1 lime and 10 soil:5 lime) and cement slurry. The potential for the use of these treatments in areas where tree roots have damaged sewers is discussed. INTRODUCTION The degree of harmony between trees and urban infrastructure depends on the levels of understanding of how, why and when tree root damage arises and what prevention strategies are available. Tree impacts on infrastructure such as sewers can be a major cost to the managers of utilities and other urban infrastructure managers (Schrock 1985; Baker, 1980; McPherson and Peper 1996). Soil disturbance during installation creates pathways for roots to grow from the surface towards the pipe. Essentially, these root growth corridors contain favourable sized pores that can hold water and air, similar those found in topsoil. If pipes have been damaged and leak, then root growth will eventually exploit such regions. Tree roots, incorrect installation procedures, other underground service operators or material degradation, may all cause damage. This paper reports on a study, which investigated the factors associated with sewer blockage in metropolitan Melbourne and which investigated techniques that could be used to minimise tree root growth in disturbed soils such as found near sewer pipes. MATERIALS AND METHODS The survey was conducted in two parts. The first was an investigation of site factors associated with tree root blockages in the year 1999 by South East Water (SEW). Proc. IC on Urban Horticulture Eds: R. Junge-Berberovic et al. Acta Hort 643, ISHS 2004 114 Sites where blockages had occurred were visited and specific information about the site recorded. The second part was an analysis of factors associated with tree root blockages that occurred in the four years 1997 to 2000. This component of the study did not involve site visits but it used the available information on blockages recorded by SEW. The study of 1999 blockages recorded data on trees, geology and soil. The study of 1997-2000 blockages examined a range of factors that may have influenced blockages. In December 1999, a site with 85 trees planted in four rows was selected at Burnley College. The trees were Eucalyptus globulus, Eucalyptus saligna, Eucalyptus camaldulensis, Eucalyptus melliodora and Populus canescens. Holes were dug the trees and then soil sections were then were backfilled with treated sandy loam soil. The treated soil section was watered and fertilised periodically to simulate a soil that is situated close to a leaking pipe. The concept of this experiment was based on studies by Nazer and Clark (1982) and Wagar and Barker (1993). There were twelve treatments (nine chemical, two physical, and one control (no treatment)). The treated cores were excavated after 177 days and the root mass in each was measured. The treatments used are displayed in Table 1. The cement slurry and compaction are categorised as ‘physical treatments’ because they act as a physical barrier to root growth. There were five replicates of each treatment.

Aspirin has a role in the prevention of cardiovascular and cerebrovascular disease, Alzheimer's dementia and several cancers. Encouraging all 50 year olds to take low-dose aspirin doubles their chances of living a healthy life into their nineties. The widespread use of aspirin, however, is limited as many older subjects are currently unable to take aspirin because of gastrointestinal side-effects. This review explores why gastrointestinal events occur with aspirin use and how a net benefit from prophylactic aspirin might be achieved in older subjects. It is suggested that, by understanding the age-related changes in upper gastrointestinal physiology and the mechanisms by which aspirin leads to the risk reductions associated with its use, it may be possible to direct interventions to improve tolerability in older subjects. This would allow greater numbers of older subjects to gain the benefits associated with aspirin use.

Recent reports by Special Olympics International and the U.S. Surgeon General have revealed significant disparities and unmet health needs encountered by persons with mental retardation and other developmental disabilities (MR/DD). Factors contributing to these disparities include deinstitutionalization, increased survival of individuals with MR/DD, lack of appropriately trained providers, and inadequate financing of dental services. To address these problems, a group of academically oriented dentists and physicians formed the American Academy of Developmental Medicine and Dentistry (AADMD). The mission of the AADMD is to improve the quality of health services provided to persons with MR/DD by improving dental and medical school-based training of dentists and enhancing clinically relevant research. A central theme of the AADMD is full collaboration between physicians and dentists in meeting its goals. The National Action Strategy developed by the AADMD focuses on creating a series of university-based Developmental Medicine and Dentistry Programs (UDMDPs) in medical and dental schools, which collaborate in service, teaching, and research with community-based primary care clinics, community hospitals, intermediate care facilities, and other private service delivery systems such as the Special Olympics Healthy Athletes Program that serve these patients. Oral-systemic interactions will receive special emphasis by the training and research programs.

Several species of bacteria have been isolated from diseased striped bass, Morone saxatilis (Walbaum), in Chesapeake Bay. Mycobacteria species are of particular interest because of their association with chronic lesions in both fish and humans. The resulting disease in fish may be characterised by emaciation, inflammation of the skin, exophthalmia (pop-eye), ascites (dropsy), open lesions, and ulceration. The prevalence of mycobacterial infection in 217 striped bass in the Maryland portion of Chesapeake Bay was determined to provide evidence of decreased overall condition of fish affected by these bacteria. The density of the granulomas was higher in the spleen and head kidney than either the heart or liver, and appeared to be most closely associated with the presence of an external lesion. Thirty-eight per cent of the fish showed some sign of an external lesion. Granulomas appeared in at least one organ in 53% of the fish sampled regardless of the presence or absence of an external skin lesion. Condition factor was significantly higher for striped bass without external lesions (0.96) than those with lesions (0.82). Striped bass with no internal granulomas and no external lesion had the highest condition factor (0.99) and fish with granulomas and external lesions the lowest (0.81). Slopes of the regression loge weight (g) and loge length (mm) for striped bass with external lesions was significantly higher than those fish without external lesions and had a lower r2. The results suggest that striped bass that are infected with mycobacteria have decreased overall health.

The human trefoil proteins TFF1 and TFF3 are expressed predominantly in the gastrointestinal tract. They are also expressed and regulated by estrogens in malignant breast epithelial cells. TFF1 and TFF3 are small cysteine-rich acidic secreted proteins of 60 and 59 amino acids with similar isoelectric points of 4.75 and 3.94, respectively. Each contains one trefoil domain that is characterized by several conserved features including six cysteine residues with conserved spacing. TFF1 and TFF3 form intermolecular disulfide bonds via an extra-trefoil domain cysteine residue and are present in vivo as monomers and homodimers and as complexes with other proteins. The TFF1 dimer is more active than the TFF1 monomer. In the present study the hydrodynamic and charge properties of TFF1 and TFF3 monomers and homodimers have been compared and shown to differ markedly. Notably, TFF1 is significantly more asymmetric than TFF3 (frictional coefficients 1.25 and 1.12, respectively, p < 0.001), and homodimerization of TFF1 results in a greater increase in asymmetry than for TFF3. The overall charges of TFF1 and TFF3 are very different at neutral pH. Titration curves predicted significant differences in charge across a wide pH range that agreed well with experimental data. The locations of charged amino acids in the primary sequences and in the tertiary structures of TFF1 and TFF3 were examined. This revealed interesting divergence in both the distribution and local topology of charged amino acid side chains. The significant differences between the shape, size, and surface charge of these two closely related molecules may account for their divergent biological activities.

We previously established that the trefoil peptides (TFFs) pS2, spasmolytic polypeptide, and intestinal trefoil factor are involved in cellular scattering and invasion in kidney and colonic cancer cells. Using the chorioallantoic membrane (CAM) assay and the formation of tube-like structures by human umbilical vein endothelial cells (HUVEC) plated on the Matrigel matrix substratum, we report here that TFFs are proangiogenic factors. Angiogenic activity of TFFs is comparable to that induced by vascular endothelial growth factor, leptin, and transforming growth factor-alpha. Stimulation of angiogenesis by pS2 in the CAM assay is blocked by pharmacological inhibitors of cyclooxygenase COX-2 (NS-398) and epidermal growth factor receptor (EGF-R) tyrosine kinase (ZD1839), but is independent of KDR/Flk-1 and thromboxane A2 receptors. In contrast, the morphogenic switch induced by pS2 in HUVEC cells could be inhibited by the specific KDR heptapeptide antagonist ATWLPPR and by inhibitors of COX-2 and EGF-R signaling. These results implicate TFFs in the formation of new blood vessels during normal and pathophysiological processes linked to wound healing, inflammation, and cancer progression in the digestive mucosa and other human solid tumors associated with aberrant expression of TFFs.

This chapter describes factors contributing to the successful planning and implementation of alternative teacher certification programs in one Texas community college district.

Students should develop self-reflection skills and appropriate views about knowledge and learning, both for their own sake and because these skills and views may be related to improvements in conceptual understanding. We explored the latter issue in the context of an introductory physics course for first-year engineering honors students. As part of the course, students submitted weekly reports, in which they reflected on how they learned specific physics content. The reports by 12 students were analyzed for the quality of reflection and some of the epistemological beliefs they exhibited. Students’ conceptual learning gains were measured with standard survey instruments. We found that students with high conceptual gains tend to show reflection on learning that is more articulate and epistemologically sophisticated than students with lower conceptual gains. Some implications for instruction are suggested.

Statement of Problem. Limiting abutment-to-implant hexagonal discrepancies and rotational movement of the abutment around the implant to less than 5 degrees would result in a more stable screw joint. However, the exact relationship after abutment screw tightening is unknown, as is the effect of a counter-torque device in limiting abutment movement during screw tightening. Purpose. This study examined the orientation of the abutment hexagon to the implant hexagon after tightening of the abutment screw for several abutment systems with and without the use of a counter-torque device. Material and Methods. Thirty conical self-tapping implants (3.75 × 10.0 mm) and 10 wide-platform Branemark System implants (5.0 × 10.0 mm), along with 10 abutment specimens from the CeraOne, Estheticone, Procera, and AuraAdapt systems, were selected for this investigation. The implants were placed in a holding device prior to tightening of the abutments. When the tightening torque recommended for each abutment system was reached with the use of a torque controller, each implant abutment specimen was removed from the holding device and embedded in a hard resin medium. The specimens were sectioned in a horizontal direction at the level of the hexagons and cleansed of debris prior to examination. The hexagon orientations were assessed as the degree and direction of rotation of the abutment hexagon around the implant hexagon. Results. The range of the maximum degrees of rotation for all 4 abutment groups tightened with or without the counter-torque device was slightly more than 3.53 degrees. The absolute degrees of rotation for all 4 abutment groups were less than 1.50 degrees with or without the use of the counter-torque device. Conclusion. The hexagon-to-hexagon orientation measured as rotational fit on all abutment systems was below the 5 degrees suggested as optimal for screw joint stability. The absolute degrees of rotation for all 4 abutment groups were less than 1.50 degrees regardless of whether the counter-torque device was used. (J Prosthet Dent 2002;87:74-9.)